Genomes and Genes
Experts and Doctors on mitochondria in Austria
- Tehlivets O, Scheuringer K, Kohlwein S. Fatty acid synthesis and elongation in yeast. Biochim Biophys Acta. 2007;1771:255-70 pubmed..In this review, we summarize what is currently known about enzymes involved in cellular fatty acid synthesis and elongation, and discuss potential links between fatty acid metabolism, physiology and cellular regulation. ..
- Rockenfeller P, Ring J, Muschett V, Beranek A, Buettner S, Carmona Gutierrez D, et al. Fatty acids trigger mitochondrion-dependent necrosis. Cell Cycle. 2010;9:2836-42 pubmed..We conclude that lipotoxicity is executed via a mitochondrial necrotic pathway, challenging the dogma that the adverse effects of lipid stress are exclusively apoptotic. ..
- Crnkovic S, Riederer M, Lechleitner M, Hallstrom S, Malli R, Graier W, et al. Docosahexaenoic acid-induced unfolded protein response, cell cycle arrest, and apoptosis in vascular smooth muscle cells are triggered by Ca²?-dependent induction of oxidative stress. Free Radic Biol Med. 2012;52:1786-95 pubmed publisher..We conclude that Ca(2+)-dependent oxidative stress is the central and initial event responsible for induction of UPR, cell cycle arrest, and apoptosis in DHA-treated hPASMCs. ..
- Nohl H, Staniek K, Kozlov A, Gille L. The biomolecule ubiquinone exerts a variety of biological functions. Biofactors. 2003;18:23-31 pubmed..Furthermore, we have addressed the antioxidant role of ubiquinone and found that the efficiency of this activity is widely dependent on the type of biomembrane where ubiquinone exerts its chain-breaking activity. ..
- Gille L, Nohl H. The ubiquinol/bc1 redox couple regulates mitochondrial oxygen radical formation. Arch Biochem Biophys. 2001;388:34-8 pubmed..The regulator which controls leakage of electrons to oxygen appears to be the electron branching activity of the bc1 complex. ..
- Nunes M, Neumeier H, Schlotterer C. Contrasting patterns of natural variation in global Drosophila melanogaster populations. Mol Ecol. 2008;17:4470-9 pubmed publisher..In the light of the well-established African origin of D. melanogaster, our results cast severe doubts about the suitability of mtDNA for biogeographic inference in this model organism. ..
- Kapitza S, Jakupec M, Uhl M, Keppler B, Marian B. The heterocyclic ruthenium(III) complex KP1019 (FFC14A) causes DNA damage and oxidative stress in colorectal tumor cells. Cancer Lett. 2005;226:115-21 pubmed..Both effects were inhibited by NAC which reduced the population with depolarized mitochondrial membranes to 24.1+/-1.2% and prevented PARP-cleavage indicating a central role oxidative stress in KP1019-induced apoptosis. ..
- Wriessnegger T, Leitner E, Belegratis M, Ingolic E, Daum G. Lipid analysis of mitochondrial membranes from the yeast Pichia pastoris. Biochim Biophys Acta. 2009;1791:166-72 pubmed publisher..In summary, our data are the first step to understand the P. pastoris lipidome which will be prerequisite to manipulate membrane components of this yeast for biotechnological purposes. ..
- Tuller G, Daum G. Import of sterols into mitochondria of the yeast Saccharomyces cerevisiae. FEBS Lett. 1995;372:29-32 pubmed
- Gaigg B, Simbeni R, Hrastnik C, Paltauf F, Daum G. Characterization of a microsomal subfraction associated with mitochondria of the yeast, Saccharomyces cerevisiae. Involvement in synthesis and import of phospholipids into mitochondria. Biochim Biophys Acta. 1995;1234:214-20 pubmed..Reassociation of MAMs with purified mitochondria led to reconstitution of the import of phosphatidylserine into mitochondria. Organelle contact is suggested as a possible mechanism of this process. ..
- Ehrenberger K. Clinical experience with caroverine in inner ear diseases. Adv Otorhinolaryngol. 2002;59:156-62 pubmed..The results of corresponding clinical trials are presented. ..
- Thyagarajan B, Malli R, Schmidt K, Graier W, Groschner K. Nitric oxide inhibits capacitative Ca2+ entry by suppression of mitochondrial Ca2+ handling. Br J Pharmacol. 2002;137:821-30 pubmed..Our data demonstrate that NO effectively inhibits CCE cells by cGMP-independent suppression of mitochondrial function. We suggest disruption of local Ca(2+) handling by mitochondria as a key mechanism of NO induced suppression of CCE. ..
- Zimmermann F, Neureiter D, Feichtinger R, Trost A, Sperl W, Kofler B, et al. Deficiency of respiratory chain complex I in Hashimoto thyroiditis. Mitochondrion. 2016;26:1-6 pubmed publisher..In addition to isolated complex I deficiency, 25% of oncocytic lesions showed combined deficiency of complex I and IV. Thus, we demonstrate for the first time a defect of respiratory chain complex I in OCs of HT patients. ..
- Koziel K, Smigelskaite J, Drasche A, Enthammer M, Ashraf M, Khalid S, et al. RAF and antioxidants prevent cell death induction after growth factor abrogation through regulation of Bcl-2 proteins. Exp Cell Res. 2013;319:2728-38 pubmed publisher..Together these data suggest Bcl-2 family proteins as convergence point for RAF and ROS in life and death decisions. ..
- Finsterer J, Stollberger C, Blazek G. Neuromuscular implications in left ventricular hypertrabeculation/noncompaction. Int J Cardiol. 2006;110:288-300 pubmed..In reverse, all patients with LVHT should undergo a comprehensive neurological investigation following the detection of LVHT. ..
- Lirk P, Haller I, Myers R, Klimaschewski L, Kau Y, Hung Y, et al. Mitigation of direct neurotoxic effects of lidocaine and amitriptyline by inhibition of p38 mitogen-activated protein kinase in vitro and in vivo. Anesthesiology. 2006;104:1266-73 pubmed
- Schneiter R, Brugger B, Sandhoff R, Zellnig G, Leber A, Lampl M, et al. Electrospray ionization tandem mass spectrometry (ESI-MS/MS) analysis of the lipid molecular species composition of yeast subcellular membranes reveals acyl chain-based sorting/remodeling of distinct molecular species en route to the plasma membrane. J Cell Biol. 1999;146:741-54 pubmed..This observation suggests that acyl chain-based sorting and/or remodeling mechanisms are operating to maintain the specific lipid molecular species composition of the yeast plasma membrane. ..
- Malli R, Frieden M, Osibow K, Graier W. Mitochondria efficiently buffer subplasmalemmal Ca2+ elevation during agonist stimulation. J Biol Chem. 2003;278:10807-15 pubmed..Thus, superficial mitochondria and ER generate local domains of low and high Ca(2+) allowing simultaneous activation of BK(Ca) and CCE, despite their opposite Ca(2+) sensitivity. ..
- Gehrke H, Frühmesser A, Pelka J, Esselen M, Hecht L, Blank H, et al. In vitro toxicity of amorphous silica nanoparticles in human colon carcinoma cells. Nanotoxicology. 2013;7:274-93 pubmed publisher..Additionally, investigations regarding DNA integrity revealed no substantial (oxidative) DNA damage. ..
- Sperka Gottlieb C, Fasch E, Kuchler K, Bailis A, Henry S, Paltauf F, et al. The hydrophilic and acidic N-terminus of the integral membrane enzyme phosphatidylserine synthase is required for efficient membrane insertion. Yeast. 1990;6:331-43 pubmed..Thus, the hydrophilic, highly acidic N-terminus of PSS is required for efficient membrane insertion but does not appear to contain sequences required for a targeting to the membrane compartment. ..
- Rossmanith W. Of P and Z: mitochondrial tRNA processing enzymes. Biochim Biophys Acta. 2012;1819:1017-26 pubmed publisher..Mitochondrial tRNA processing thus appears dominated by new, eukaryal inventions rather than bacterial heritage. This article is part of a Special Issue entitled: Mitochondrial Gene Expression. ..
- Fischer M, Sharma R, Lim J, Haider L, Frischer J, Drexhage J, et al. NADPH oxidase expression in active multiple sclerosis lesions in relation to oxidative tissue damage and mitochondrial injury. Brain. 2012;135:886-99 pubmed publisher..Our data suggest that the inflammation-associated oxidative burst in activated microglia and macrophages plays an important role in demyelination and free radical-mediated tissue injury in the pathogenesis of multiple sclerosis. ..
- Bernhard D, Schwaiger W, Crazzolara R, Tinhofer I, Kofler R, Csordas A. Enhanced MTT-reducing activity under growth inhibition by resveratrol in CEM-C7H2 lymphocytic leukemia cells. Cancer Lett. 2003;195:193-9 pubmed..Thus, in determining the effects of flavonoids and polyphenols on proliferation, in certain cell types this might represent a pitfall in the MTT proliferation assay. ..
- Modre Osprian R, Osprian I, Tilg B, Schreier G, Weinberger K, Graber A. Dynamic simulations on the mitochondrial fatty acid beta-oxidation network. BMC Syst Biol. 2009;3:2 pubmed publisher..Our findings offer insight into the disease process (e.g. rapid progress to coma) and might confirm new explanations (no human cases of LCAD deficiency), which can hardly be obtained from experimental data alone. ..
- Krumschnabel G, Nawaz M. Acute toxicity of hexavalent chromium in isolated teleost hepatocytes. Aquat Toxicol. 2004;70:159-67 pubmed..Overall, our data show that Cr(VI) is acutely toxic to goldfish hepatocytes, and its toxicity is associated with the induction of radical stress, presumably involving lysosomes and mitochondria as important sources of ROS formation. ..
- Kohlwein S, Kuchler K, Sperka Gottlieb C, Henry S, Paltauf F. Identification of mitochondrial and microsomal phosphatidylserine synthase in Saccharomyces cerevisiae as the gene product of the CHO1 structural gene. J Bacteriol. 1988;170:3778-81 pubmed..In the absence of protease inhibitors during the preparation of membranes, the enzyme underwent degradation to an enzymatically active protein of 23,000 apparent molecular weight. ..
- Beranek A, Rechberger G, Knauer H, Wolinski H, Kohlwein S, Leber R. Identification of a cardiolipin-specific phospholipase encoded by the gene CLD1 (YGR110W) in yeast. J Biol Chem. 2009;284:11572-8 pubmed publisher..We therefore rename the Ygr110wp as Cld1p (cardiolipin-specific deacylase 1). ..
- ..Molecular aspects of these processes as well as diseases related to defects in the formation of mitochondrial membranes will be described. ..
- Zinser E, Sperka Gottlieb C, Fasch E, Kohlwein S, Paltauf F, Daum G. Phospholipid synthesis and lipid composition of subcellular membranes in the unicellular eukaryote Saccharomyces cerevisiae. J Bacteriol. 1991;173:2026-34 pubmed..The rigidity of the plasma membrane and of secretory vesicles, determined by measuring fluorescence anisotropy by using trimethylammonium diphenylhexatriene as a probe, can be attributed to the high content of ergosterol. ..
- Rinnerthaler M, Jarolim S, Heeren G, Palle E, Perju S, Klinger H, et al. MMI1 (YKL056c, TMA19), the yeast orthologue of the translationally controlled tumor protein (TCTP) has apoptotic functions and interacts with both microtubules and mitochondria. Biochim Biophys Acta. 2006;1757:631-8 pubmed..Our results so far indicate that Mmi1p is one of the few proteins establishing a functional link between microtubules and mitochondria which may be needed for correct localization of mitochondria during cell division. ..
- Bui D, Gregan J, Jarosch E, Ragnini A, Schweyen R. The bacterial magnesium transporter CorA can functionally substitute for its putative homologue Mrs2p in the yeast inner mitochondrial membrane. J Biol Chem. 1999;274:20438-43 pubmed..Mutants lacking or overproducing Mrs2p exhibit phenotypes consistent with the involvement of Mrs2p in mitochondrial Mg2+ homeostasis. ..
- Rezniczek G, Abrahamsberg C, Fuchs P, Spazierer D, Wiche G. Plectin 5'-transcript diversity: short alternative sequences determine stability of gene products, initiation of translation and subcellular localization of isoforms. Hum Mol Genet. 2003;12:3181-94 pubmed..Thus, with plectin as a model, we demonstrate a role for 5'-untranslated regions and alternative 5'-splicing as an important regulatory mechanism of protein expression and protein function. ..
- Tudzarova Trajkovska S, Wesierska Gadek J. Strong induction of p73 protein in vivo coincides with the onset of apoptosis in rat liver after treatment with the hepatocarcinogen N-nitrosomorpholine (NNM). J Cell Biochem. 2003;90:837-55 pubmed..Our data indicate that activated p73 protein may positively affect the induction and execution of apoptosis in response to genotoxic action of NNM. ..
- Birner R, Nebauer R, Schneiter R, Daum G. Synthetic lethal interaction of the mitochondrial phosphatidylethanolamine biosynthetic machinery with the prohibitin complex of Saccharomyces cerevisiae. Mol Biol Cell. 2003;14:370-83 pubmed..In conclusion, destabilizing effects triggered by low levels of mitochondrial PtdEtn seem to account for synthetic lethality of psd1Delta with phb mutants. ..
- Zotova L, Aleschko M, Sponder G, Baumgartner R, Reipert S, Prinz M, et al. Novel components of an active mitochondrial K(+)/H(+) exchange. J Biol Chem. 2010;285:14399-414 pubmed publisher..Based on these results, we propose that Ydl183p and the Mdm38p homologues Mrs7p, LETM1, and HCCR-1 are involved in the formation of an active KHE system. ..
- Tatzer V, Zellnig G, Kohlwein S, Schneiter R. Lipid-dependent subcellular relocalization of the acyl chain desaturase in yeast. Mol Biol Cell. 2002;13:4429-42 pubmed..A model is discussed in which the accumulation of saturated lipids in a microdomain around the desaturase could induce the observed segregation and relocalization of the enzyme. ..
- Steinkellner T, Freissmuth M, Sitte H, Montgomery T. The ugly side of amphetamines: short- and long-term toxicity of 3,4-methylenedioxymethamphetamine (MDMA, 'Ecstasy'), methamphetamine and D-amphetamine. Biol Chem. 2011;392:103-15 pubmed publisher..Here, we attempt to review amphetamine toxicity and in particular MDMA toxicity in the pathogenesis of exemplary human pathologies, independently of confounding environmental factors such as poly-drug use and drug purity...
- Steinborn R, Schinogl P, Wells D, Bergthaler A, M ller M, Brem G. Coexistence of Bos taurus and B. indicus mitochondrial DNAs in nuclear transfer-derived somatic cattle clones. Genetics. 2002;162:823-9 pubmed..In addition, intrasubspecific B. indicus heteroplasmy of approximately 1% (but up to 7.3 and 12.7% in muscle and follicular cells of one animal) was detected in 7 out of the 9 B. indicus intrasubspecific cloned cattle...
- Thyagarajan B, Poteser M, Romanin C, Kahr H, Zhu M, Groschner K. Expression of Trp3 determines sensitivity of capacitative Ca2+ entry to nitric oxide and mitochondrial Ca2+ handling: evidence for a role of Trp3 as a subunit of capacitative Ca2+ entry channels. J Biol Chem. 2001;276:48149-58 pubmed..Moreover, we show for the first time that Trp3 expression determines biophysical properties as well as regulation of CCE channels by NO and mitochondrial Ca(2+) handling. Thus, we propose Trp3 as a subunit of CCE channels...
- Pfister G, Stroh C, Perschinka H, Kind M, Knoflach M, Hinterdorfer P, et al. Detection of HSP60 on the membrane surface of stressed human endothelial cells by atomic force and confocal microscopy. J Cell Sci. 2005;118:1587-94 pubmed..The relevance of our findings with respect to the role of HSP60 in atherogenesis is discussed. ..
- Konieczny P, Fuchs P, Reipert S, Kunz W, Zeöld A, Fischer I, et al. Myofiber integrity depends on desmin network targeting to Z-disks and costameres via distinct plectin isoforms. J Cell Biol. 2008;181:667-81 pubmed publisher..Our findings establish plectin as the major organizer of desmin IFs in myofibers and provide new insights into plectin- and desmin-related muscular dystrophies. ..
- Piskacek M, Zotova L, Zsurka G, Schweyen R. Conditional knockdown of hMRS2 results in loss of mitochondrial Mg(2+) uptake and cell death. J Cell Mol Med. 2009;13:693-700 pubmed publisher..We conclude that hMrs2 is the major transport protein for Mg (+) uptake into mitochondria and that expression of hMrs2 is essential for the maintenance of respiratory complex I and cell viability. ..
- Horvath S, Böttinger L, Vögtle F, Wiedemann N, Meisinger C, Becker T, et al. Processing and topology of the yeast mitochondrial phosphatidylserine decarboxylase 1. J Biol Chem. 2012;287:36744-55 pubmed publisher..In summary, membrane integration of Psd1 is crucial for its functionality and for maintenance of mitochondrial lipid homeostasis. ..
- Pircher H, Straganz G, Ehehalt D, Morrow G, Tanguay R, Jansen Durr P. Identification of human fumarylacetoacetate hydrolase domain-containing protein 1 (FAHD1) as a novel mitochondrial acylpyruvase. J Biol Chem. 2011;286:36500-8 pubmed publisher..FAHD1 was also found in several human cell lines, where it localized to mitochondria. In summary, the current work identified mammalian FAHD1 as a novel mitochondrial enzyme with acylpyruvate hydrolase activity. ..
- Schmölzer P, Höftberger M, Foissner I. Plasma membrane domains participate in pH banding of Chara internodal cells. Plant Cell Physiol. 2011;52:1274-88 pubmed publisher..Cortical mitochondria were also larger and more abundant at the acid bands, and their intimate association with charasomes and chloroplasts suggests an involvement in carbon uptake and photorespiration. ..
- Jarosch E, Tuller G, Daum G, Waldherr M, Voskova A, Schweyen R. Mrs5p, an essential protein of the mitochondrial intermembrane space, affects protein import into yeast mitochondria. J Biol Chem. 1996;271:17219-25 pubmed..Depletion of Mrs5p in yeast cells causes accumulation of unprocessed precursors of the mitochondrial hsp60 protein and defects in all cytochrome complexes. These findings suggest an essential role of Mrs5p in mitochondrial biogenesis. ..
- Ragnini A, Teply R, Waldherr M, Voskova A, Schweyen R. The yeast protein Mrs6p, a homologue of the rabGDI and human choroideraemia proteins, affects cytoplasmic and mitochondrial functions. Curr Genet. 1994;26:308-14 pubmed..We conclude from these results that the MRS6 gene product has a vital function in connection with Rab/Ypt-type proteins in the cytoplasm and, in addition, affects mitochondrial functions. ..
- Weghuber J, Dieterich F, Froschauer E, Svidova S, Schweyen R. Mutational analysis of functional domains in Mrs2p, the mitochondrial Mg2+ channel protein of Saccharomyces cerevisiae. FEBS J. 2006;273:1198-209 pubmed..Data presented support the notion that effects of mutations in Mrs2p on group II intron splicing are a consequence of changes in steady-state mitochondrial Mg2+ concentrations. ..
- Wesierska Gadek J, Gueorguieva M, Horky M. Roscovitine-induced up-regulation of p53AIP1 protein precedes the onset of apoptosis in human MCF-7 breast cancer cells. Mol Cancer Ther. 2005;4:113-24 pubmed..Remarkably, roscovitine failed to induce phosphorylation at Ser46 in caspase-3-reconstituted MCF-7 cells. Our results indicate that, depending on the status of caspase-3 in MCF-7 cells, different apoptotic pathways were initialized. ..
- Nebauer R, Schuiki I, Kulterer B, Trajanoski Z, Daum G. The phosphatidylethanolamine level of yeast mitochondria is affected by the mitochondrial components Oxa1p and Yme1p. FEBS J. 2007;274:6180-90 pubmed..In summary, our results demonstrate a link between the mitochondrial protein import machinery, assembly and stability of Psd1p, and phosphatidylethanolamine homeostasis in yeast mitochondria. ..
- Lechner J, Malloth N, Seppi T, Beer B, Jennings P, Pfaller W. IFN-alpha induces barrier destabilization and apoptosis in renal proximal tubular epithelium. Am J Physiol Cell Physiol. 2008;294:C153-60 pubmed..Caspase-dependent and -independent apoptotic mechanisms are involved. These findings reveal a novel aspect of IFN-alpha actions with implications for normal renal function in immune reactions and during IFN-alpha therapy. ..
- Wiesenberger G, Speer F, Haller G, Bonnefoy N, Schleiffer A, Schafer B. RNA degradation in fission yeast mitochondria is stimulated by a member of a new family of proteins that are conserved in lower eukaryotes. J Mol Biol. 2007;367:681-91 pubmed
- Froschauer E, Nowikovsky K, Schweyen R. Electroneutral K+/H+ exchange in mitochondrial membrane vesicles involves Yol027/Letm1 proteins. Biochim Biophys Acta. 2005;1711:41-8 pubmed..Considering their newly identified function, we propose to refer to the yeast YOL027c gene and the human LETM1 gene as yMKH1 and hMKH1, respectively. ..
- Kolisek M, Zsurka G, Samaj J, Weghuber J, Schweyen R, Schweigel M. Mrs2p is an essential component of the major electrophoretic Mg2+ influx system in mitochondria. EMBO J. 2003;22:1235-44 pubmed..Taken together, these findings characterize Mrs2p as the first molecularly identified metal ion channel protein in the inner mitochondrial membrane. ..
- Kuznetsov A, Veksler V, Gellerich F, Saks V, Margreiter R, Kunz W. Analysis of mitochondrial function in situ in permeabilized muscle fibers, tissues and cells. Nat Protoc. 2008;3:965-76 pubmed publisher..As only a small amount of tissue is required for analysis, the protocol can be used in diagnostic settings in clinical studies. The permeabilization procedure and specific titration analysis can be completed in 2 h...
- Bürgermeister M, Birner Grünberger R, Nebauer R, Daum G. Contribution of different pathways to the supply of phosphatidylethanolamine and phosphatidylcholine to mitochondrial membranes of the yeast Saccharomyces cerevisiae. Biochim Biophys Acta. 2004;1686:161-8 pubmed..Thus, the different pathways of PtdEtn biosynthesis play different roles in the assembly of PtdEtn into cellular membranes...
- Nowikovsky K, Schweyen R, Bernardi P. Pathophysiology of mitochondrial volume homeostasis: potassium transport and permeability transition. Biochim Biophys Acta. 2009;1787:345-50 pubmed publisher..Specific emphasis will be placed on molecular advances on the nature of the transport protein(s) involved, and/or on diseases that depend on mitochondrial volume dysregulation. ..
- Radad K, Gille G, Moldzio R, Saito H, Rausch W. Ginsenosides Rb1 and Rg1 effects on mesencephalic dopaminergic cells stressed with glutamate. Brain Res. 2004;1021:41-53 pubmed..Thus our study indicates that ginsenosides Rb1 and Rg1 have a partial neurotrophic and neuroprotective role in dopaminergic cell culture. ..
- Nowikovsky K, Froschauer E, Zsurka G, Samaj J, Reipert S, Kolisek M, et al. The LETM1/YOL027 gene family encodes a factor of the mitochondrial K+ homeostasis with a potential role in the Wolf-Hirschhorn syndrome. J Biol Chem. 2004;279:30307-15 pubmed..Thus, YOL027/LETM1 are the first genes shown to encode factors involved in both K+ homeostasis and organelle volume control...
- Zimmermann F, Mayr J, Neureiter D, Feichtinger R, Alinger B, Jones N, et al. Lack of complex I is associated with oncocytic thyroid tumours. Br J Cancer. 2009;100:1434-7 pubmed publisher..A specific lack of complex I was detected, which was expressed at <5% of the level determined in surrounding non-cancerous tissue. ..
- Gregan J, Bui D, Pillich R, Fink M, Zsurka G, Schweyen R. The mitochondrial inner membrane protein Lpe10p, a homologue of Mrs2p, is essential for magnesium homeostasis and group II intron splicing in yeast. Mol Gen Genet. 2001;264:773-81 pubmed..However, they cannot easily substitute for each other. Their roles in magnesium homeostasis and, possibly as a secondary effect, in RNA splicing are discussed. ..
- Wiesenberger G, Link T, von Ahsen U, Waldherr M, Schweyen R. MRS3 and MRS4, two suppressors of mtRNA splicing defects in yeast, are new members of the mitochondrial carrier family. J Mol Biol. 1991;217:23-37 pubmed..The findings are discussed in relation to possible functions of the MRS proteins in mitochondrial solute translocation and RNA splicing. ..
- Bito A, Haider M, Hadler I, Breitenbach M. Identification and phenotypic analysis of two glyoxalase II encoding genes from Saccharomyces cerevisiae, GLO2 and GLO4, and intracellular localization of the corresponding proteins. J Biol Chem. 1997;272:21509-19 pubmed..However, to get an active Glo4p protein in E. coli, the putative mitochondrial transit peptide at the N-terminal end had to be removed by shortening the 5' end of the GLO4 open reading frame. ..
- Linke M, Fritsch S, Sukhbaatar N, Hengstschlager M, Weichhart T. mTORC1 and mTORC2 as regulators of cell metabolism in immunity. FEBS Lett. 2017;591:3089-3103 pubmed publisher..Elucidation of the metabolic control mechanisms of immune responses will help to generate a systemic understanding of the immune system. ..
- Ullen A, Fauler G, Bernhart E, Nusshold C, Reicher H, Leis H, et al. Phloretin ameliorates 2-chlorohexadecanal-mediated brain microvascular endothelial cell dysfunction in vitro. Free Radic Biol Med. 2012;53:1770-81 pubmed publisher..These data suggest that 2-ClHDA has the potential to induce BBB breakdown under inflammatory conditions and that phloretin confers protection in this experimental setting...
- Jesenberger V, Procyk K, Ruth J, Schreiber M, Theussl H, Wagner E, et al. Protective role of Raf-1 in Salmonella-induced macrophage apoptosis. J Exp Med. 2001;193:353-64 pubmed..Instead, pathogen-mediated activation of caspase-1 is enhanced selectively, implying that Raf-1 antagonizes stimulus-induced caspase-1 activation and apoptosis. ..
- Gregan J, Kolisek M, Schweyen R. Mitochondrial Mg(2+) homeostasis is critical for group II intron splicing in vivo. Genes Dev. 2001;15:2229-37 pubmed..It explains finally why protein factors modulating Mg(2+) homeostasis had been identified in genetic screens for bona fide RNA splicing factors. ..
- Kapitza S, Pongratz M, Jakupec M, Heffeter P, Berger W, Lackinger L, et al. Heterocyclic complexes of ruthenium(III) induce apoptosis in colorectal carcinoma cells. J Cancer Res Clin Oncol. 2005;131:101-10 pubmed..This paper examines the cell biological mechanisms underlying their antineoplastic effects...
- Dreyer H, Steiner G. The complete sequence and gene organization of the mitochondrial genome of the gadilid scaphopod Siphonondentalium lobatum (Mollusca). Mol Phylogenet Evol. 2004;31:605-17 pubmed publisher..Moreover, assessing the phylogenetic signal in gene order data requires a much larger taxon sample than is currently available, given the exceptional diversity of this character set in the Mollusca...