Experts and Doctors on lyases in Spain

Summary

Locale: Spain
Topic: lyases

Top Publications

  1. Puente Polledo L, Reglero A, González Clemente C, Rodriguez Aparicio L, Ferrero M. Biochemical conditions for the production of polysialic acid by Pasteurella haemolytica A2. Glycoconj J. 1998;15:855-61 pubmed
    ..5 mM, respectively, and finally a membrane-associated polysialyltransferase, which catalyzes the incorporation of sialic acid from CMP-Neu5Ac into polymeric products with an apparent CMP-Neu5Ac Km of 250 microM. ..
  2. Malumbres M, Mateos L, Guerrero C, Martin J. Nucleotide sequence of the threonine synthase (thrC) gene of Brevibacterium lactofermentum. Nucleic Acids Res. 1988;16:9859 pubmed
  3. Ramos C, Calderon I. Biochemical evidence that the Saccharomyces cerevisiae THR4 gene encodes threonine synthetase. FEBS Lett. 1994;351:357-9 pubmed
    ..Using this method we have determined that this activity depends on the presence in the cell of an active form of the THR4 gene, thus demonstrating the univocal relationship between them. ..
  4. Botet J, Mateos L, Revuelta J, Santos M. A chemogenomic screening of sulfanilamide-hypersensitive Saccharomyces cerevisiae mutants uncovers ABZ2, the gene encoding a fungal aminodeoxychorismate lyase. Eukaryot Cell. 2007;6:2102-11 pubmed
    ..Abz2p appeared to form homodimers and dimerization was indispensable for its catalytic activity. ..
  5. Malumbres M, Mateos L, Lumbreras M, Guerrero C, Martin J. Analysis and expression of the thrC gene of Brevibacterium lactofermentum and characterization of the encoded threonine synthase. Appl Environ Microbiol. 1994;60:2209-19 pubmed
    ..8-kDa protein, as shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The purified enzyme required pyridoxal phosphate as its only cofactor to convert homoserine phosphate into threonine. ..
  6. Malumbres M, Mateos L, Guerrero C, Martin J. Molecular cloning of the hom-thrC-thrB cluster from Bacillus sp. ULM1: expression of the thrC gene in Escherichia coli and corynebacteria, and evolutionary relationships of the threonine genes. Folia Microbiol (Praha). 1995;40:595-606 pubmed
    ..Amino acid comparison of nine threonine synthases revealed evolutionary relationships between different groups of bacteria. ..
  7. Bermúdez M, Páez Ochoa M, Gotor C, Romero L. Arabidopsis S-sulfocysteine synthase activity is essential for chloroplast function and long-day light-dependent redox control. Plant Cell. 2010;22:403-16 pubmed publisher
    ..Our findings reveal that S-sulfocysteine and the activity of S-sulfocysteine synthase play important roles in chloroplast function and are essential for light-dependent redox regulation within the chloroplast. ..
  8. Zafrilla B, Mart nez Espinosa R, Esclapez J, P rez Pomares F, Bonete M. SufS protein from Haloferax volcanii involved in Fe-S cluster assembly in haloarchaea. Biochim Biophys Acta. 2010;1804:1476-82 pubmed publisher
    ..In this study, we have cloned, expressed and characterised a cysteine desulphurase (SufS) from Haloferax volcanii and demonstrated that this protein is able to reconstitute the [Fe-S] cluster of halophilic ferredoxin...
  9. Ferrer S, Martí S, Moliner V, Tuñón I, Bertran J. Understanding the different activities of highly promiscuous MbtI by computational methods. Phys Chem Chem Phys. 2012;14:3482-9 pubmed publisher
    ..Mg(2+) would be polarizing and pre-organizing the substrate and active site, as well as shifting the pK(a) values of key active site residues. ..

More Information

Publications10

  1. Bermúdez M, Galmés J, Moreno I, Mullineaux P, Gotor C, Romero L. Photosynthetic adaptation to length of day is dependent on S-sulfocysteine synthase activity in the thylakoid lumen. Plant Physiol. 2012;160:274-88 pubmed publisher
    ..This finding was reinforced by the observation of marked alterations in many lumenal proteins in the cs26 mutant compared with the wild type. ..