Experts and Doctors on liver microsomes in France


Locale: France
Topic: liver microsomes

Top Publications

  1. Cabaton N, Zalko D, Rathahao E, Canlet C, Delous G, Chagnon M, et al. Biotransformation of bisphenol F by human and rat liver subcellular fractions. Toxicol In Vitro. 2008;22:1697-704 pubmed publisher
    ..Both the formation of a catechol and of dimeric metabolites correspond to biotransformation pathways shared by BPF, other bisphenols and estradiol. ..
  2. Le Quéré V, Plée Gautier E, Potin P, Madec S, Salaun J. Human CYP4F3s are the main catalysts in the oxidation of fatty acid epoxides. J Lipid Res. 2004;45:1446-58 pubmed
    ..These findings demonstrate that another pathway besides conversion to vicinal diol or chain shortening by beta-oxidation exists for fatty acid epoxide inactivation. ..
  3. Le Gal A, Dreano Y, Gervasi P, Berthou F. Human cytochrome P450 2A6 is the major enzyme involved in the metabolism of three alkoxyethers used as oxyfuels. Toxicol Lett. 2001;124:47-58 pubmed
  4. Cansell M, Battin A, Degrace P, Gresti J, Clouet P, Combe N. Early dissimilar fates of liver eicosapentaenoic acid in rats fed liposomes or fish oil and gene expression related to lipid metabolism. Lipids. 2009;44:237-47 pubmed publisher
    ..The quasi absence of EPA in hepatic PL of liposome-treated rats on the short term could result from increased beta-oxidation activities through metabolic regulations induced by more available free EPA and other PUFA. ..
  5. Bournique B, Lemarié A. Docetaxel (Taxotere) is not metabolized by recombinant human CYP1B1 in vitro, but acts as an effector of this isozyme. Drug Metab Dispos. 2002;30:1149-52 pubmed
    ..In conclusion, docetaxel was not metabolized by recombinant human CYP1B1 in vitro, under any of the conditions tested. Docetaxel was shown to bind to recombinant human CYP1B1 and to act as an effector of this enzyme. ..
  6. Soraci A, Benoit E. In vitro fenoprofenyl-coenzyme A thioester formation: interspecies variations. Chirality. 1995;7:534-40 pubmed
    ..Furthermore palmitic acid inhibited (-)-(R)-fenoprofen-CoA formation in the same extent in all animal species. The stereoselectivity of the thioesterification was also species dependent. ..
  7. Jacques C, Perdu E, Duplan H, Jamin E, Canlet C, Debrauwer L, et al. Disposition and biotransformation of 14C-Benzo(a)pyrene in a pig ear skin model: ex vivo and in vitro approaches. Toxicol Lett. 2010;199:22-33 pubmed publisher
    ..This ex vivo model, which combines a functional skin barrier and active biotransformation capabilities, appears to represent a valuable alternative tool in transdermal exposure studies...
  8. Souidi M, Tissandie E, Grandcolas L, Grison S, Paquet F, Voisin P, et al. Chronic contamination with 137cesium in rat: effect on liver cholesterol metabolism. Int J Toxicol. 2006;25:493-7 pubmed
    ..These results suggest that chronic long term exposure at low-level of 137Cs may evolve to lipid disorder...
  9. Pichard Garcia L, Weaver R, Eckett N, Scarfe G, Fabre J, Lucas C, et al. The olivacine derivative s 16020 (9-hydroxy-5,6-dimethyl-N-[2-(dimethylamino)ethyl)-6H-pyrido(4,3-B)-carbazole-1-carboxamide) induces CYP1A and its own metabolism in human hepatocytes in primary culture. Drug Metab Dispos. 2004;32:80-8 pubmed

More Information


  1. Barbier O, Villeneuve L, Bocher V, Fontaine C, Torra I, Duhem C, et al. The UDP-glucuronosyltransferase 1A9 enzyme is a peroxisome proliferator-activated receptor alpha and gamma target gene. J Biol Chem. 2003;278:13975-83 pubmed
    ..Furthermore, since UGT1A9 is involved in the catabolism of fibrates, these results suggest that PPAR alpha and PPAR gamma may control the intracellular level of active fibrates. ..
  2. Le Goff Klein N, Koffel J, Jung L, Ubeaud G. In vitro inhibition of simvastatin metabolism, a HMG-CoA reductase inhibitor in human and rat liver by bergamottin, a component of grapefruit juice. Eur J Pharm Sci. 2003;18:31-5 pubmed
    ..In conclusion, our study shows that BG inhibits SV metabolism. BG and NRG could therefore be applied as markers in food-drug interaction studies in order to adjust posology...
  3. Haag M, Leusink Muis T, Le Bouquin R, Nijkamp F, Lugnier A, Frossard N, et al. Increased expression and decreased activity of cytochrome P450 1A1 in a murine model of toluene diisocyanate-induced asthma. Arch Toxicol. 2002;76:621-7 pubmed
    ..However, this effect is associated with a decreased enzyme activity, which might limit the toxicological consequences of increased Cyp1a1 expression. ..
  4. Lejus C, Fautrel A, Malledant Y, Guillouzo A. Inhibition of cytochrome P450 2E1 by propofol in human and porcine liver microsomes. Biochem Pharmacol. 2002;64:1151-6 pubmed
    ..These in vitro results suggest that propofol could have a protective effect on toxic metabolite activation of compounds catalyzed by CYP2E1. ..
  5. Kessler M, Ubeaud G, Walter T, Sturm F, Jung L. Free radical scavenging and skin penetration of troxerutin and vitamin derivatives. J Dermatolog Treat. 2002;13:133-41 pubmed
    ..Thus, this association can improve skin care products for preventing free radical-mediated damage. ..
  6. Lattard V, Longin Sauvageon C, Lachuer J, Delatour P, Benoit E. Cloning, sequencing, and tissue-dependent expression of flavin-containing monooxygenase (FMO) 1 and FMO3 in the dog. Drug Metab Dispos. 2002;30:119-28 pubmed
    ..By Northern blotting, the probe for FMO1 specifically hybridized a 2.6-kilobase (kb) transcript in liver and lung samples only. The probe for FMO3 hybridized two transcripts of approximately 3 and 4.2 kb in the liver and lung samples. ..
  7. Treluyer J, Rey E, Sonnier M, Pons G, Cresteil T. Evidence of impaired cisapride metabolism in neonates. Br J Clin Pharmacol. 2001;52:419-25 pubmed
    ..Owing to the difficulties associated with in vivo pharmacokinetic studies in a paediatric population, we explored the in vitro metabolism of cisapride by human cytochrome P450...
  8. Haberkorn V, Heydel J, Mounie J, Artur Y, Goudonnet H. Influence of vitamin A status on the regulation of uridine (5'-)diphosphate-glucuronosyltransferase (UGT) 1A1 and UGT1A6 expression by L-triiodothyronine. Br J Nutr. 2001;85:289-97 pubmed
    ..Our results demonstrate for the first time the existence of a strong interaction between vitamin A and thyroid hormone on the regulation of genes encoding cellular detoxification enzymes, in this case the UGT. ..
  9. Poli Scaife S, Attias R, Dansette P, Mansuy D. The substrate binding site of human liver cytochrome P450 2C9: an NMR study. Biochemistry. 1997;36:12672-82 pubmed
    ..Sulfaphenazole was easily included in that model; its very high affinity for CYP 2C9 is due to a third structural feature, the presence of its NH2 function which binds to CYP 2C9 iron. ..
  10. Jean P, Lopez Garcia P, Dansette P, Mansuy D, Goldstein J. Oxidation of tienilic acid by human yeast-expressed cytochromes P-450 2C8, 2C9, 2C18 and 2C19. Evidence that this drug is a mechanism-based inhibitor specific for cytochrome P-450 2C9. Eur J Biochem. 1996;241:797-804 pubmed
    ..Experiments performed with human liver microsomes confirmed that tienilic acid 5-hydroxylase underwent a time-dependent inactivation (apparent t1/2 = 10 +/- 5 min) during 5-hydroxylation of tienilic acid. ..
  11. Buronfosse T, Moroni P, Benoit E, Riviere J. Stereoselective sulfoxidation of the pesticide methiocarb by flavin-containing monooxygenase and cytochrome P450-dependent monooxygenases of rat liver microsomes. Anticholinesterase activity of the two sulfoxide enantiomers. J Biochem Toxicol. 1995;10:179-89 pubmed
    ..216 microM-1.min-1) than methiocarb, but a 10-fold difference was observed between the bimolecular rate constants found for the two sulfoxides produced (0.054 and 0.502 microM-1.min-1 for the (A) and (B) enantiomers, respectively)...
  12. Mortaud S, Donsez Darcel E, Roubertoux P, Degrelle H. Murine steroid sulfatase (mSTS): purification, characterization and measurement by ELISA. J Steroid Biochem Mol Biol. 1995;52:91-6 pubmed
    ..Results in ELISA confirmed the polymorphism previously demonstrated for an enzymatic mSTS activity assay in two inbred mouse strains. ..
  13. Broly F, Libersa C, Lhermitte M, Dupuis B. Inhibitory studies of mexiletine and dextromethorphan oxidation in human liver microsomes. Biochem Pharmacol. 1990;39:1045-53 pubmed
    ..These data suggest that the formation of two major metabolites of mexiletine is predominantly catalysed by the genetically variable human liver P-450dbl. ..
  14. Savelli J, Narce M, Fustier V, Poisson J. Desaturase activities are depleted before and after weaning in liver microsomes of spontaneously hypertensive rats. Prostaglandins Leukot Essent Fatty Acids. 2002;66:541-7 pubmed
    ..Such a loss of desaturase activities may be under the control of hormones involved in the regulation of SHR blood pressure. ..
  15. Bonierbale E, Valadon P, Pons C, Desfosses B, Dansette P, Mansuy D. Opposite behaviors of reactive metabolites of tienilic acid and its isomer toward liver proteins: use of specific anti-tienilic acid-protein adduct antibodies and the possible relationship with different hepatotoxic effects of the two compounds. Chem Res Toxicol. 1999;12:286-96 pubmed
  16. Benoit E, Delatour P, Olivier L, Caldwell J. (-)-R-fenoprofen: formation of fenoprofenyl-coenzyme A by rat liver microsomes. Biochem Pharmacol. 1995;49:1717-20 pubmed
    ..The microsomal long-chain fatty acid CoA ligase was markedly enantioselective towards (-)-R-FPF and the formation of (-)-R-FP-CoA was inhibited by both the (+)-S enantiomer and palmitic acid. ..
  17. Soraci A, Benoit E, Delatour P. Comparative metabolism of R(-)-fenoprofen in rats and sheep. J Vet Pharmacol Ther. 1995;18:167-71 pubmed
    ..In consequence, in rats other metabolic pathways for R(-)-FPF-CoA, such as incorporation into triacylglycerols and conjugation with amino acids, may be quantitatively more important. ..