Experts and Doctors on hydrogen ion concentration in Canada


Locale: Canada
Topic: hydrogen ion concentration

Top Publications

  1. Drummelsmith J, Girard I, Trudel N, Ouellette M. Differential protein expression analysis of Leishmania major reveals novel roles for methionine adenosyltransferase and S-adenosylmethionine in methotrexate resistance. J Biol Chem. 2004;279:33273-80 pubmed
    ..This thorough comparative proteomic analysis highlights the variety of responses required for drug resistance to be achieved. ..
  2. Champagne C, Tompkins T, Buckley N, Green Johnson J. Effect of fermentation by pure and mixed cultures of Streptococcus thermophilus and Lactobacillus helveticus on isoflavone and B-vitamin content of a fermented soy beverage. Food Microbiol. 2010;27:968-72 pubmed publisher
    ..longum R0175 had no significant effect on isoflavone levels. Combining a S. thermophilus strain with a L. helveticus culture reduced the effectiveness of the latter. Fermentation did not significantly modify vitamin B1 or B6 levels. ..
  3. Maidorn R, Cragoe E, Tannock I. Therapeutic potential of analogues of amiloride: inhibition of the regulation of intracellular pH as a possible mechanism of tumour selective therapy. Br J Cancer. 1993;67:297-303 pubmed
  4. Beveridge T, Harrison J, Drover J. Processing effects on the composition of sea buckthorn juice from Hippophae rhamnoides L. Cv. Indian Summer. J Agric Food Chem. 2002;50:113-6 pubmed
    ..Washing berries by dipping reduced soluble solids (degrees Brix) in juice suggesting uptake of wash water. ..
  5. Casey J, Grinstein S, Orlowski J. Sensors and regulators of intracellular pH. Nat Rev Mol Cell Biol. 2010;11:50-61 pubmed publisher
    ..A dynamic, finely tuned balance between proton-extruding and proton-importing processes underlies pH homeostasis not only in the cytosol, but in other cellular compartments as well. ..
  6. Buckley W, Vanderpool R. Analytical variables affecting exchangeable copper determination in blood plasma. Biometals. 2008;21:601-12 pubmed publisher
    ..Ultrafiltration of plasma yielded a Cu fraction about half that of the exchangeable Cu fraction. We conclude that the stable isotope dilution procedure for plasma exchangeable Cu yields reliable, physiologically meaningful results. ..
  7. Coady M, Chang M, Charron F, Plata C, Wallendorff B, Sah J, et al. The human tumour suppressor gene SLC5A8 expresses a Na+-monocarboxylate cotransporter. J Physiol. 2004;557:719-31 pubmed
    ..The significance of a Na(+)-monocarboxylate transporter to colon cancer presumably stems from the transport of butyrate, which is well known for having anti-proliferative and apoptosis-inducing activity in colon epithelial cells. ..
  8. Petre C, Larachi F. Capillary electrophoretic separation of inorganic sulfur-sulfide, polysulfides, and sulfur-oxygen species. J Sep Sci. 2006;29:144-52 pubmed
    ..Analysis of the oxidation reaction products provided retrospectively tentative evidence that the peaks separated and identified as tri- and tetrasulfide may be ascribed to polysulfides. ..
  9. Gingras D, Menard P, Beliveau R. Protein carboxyl methylation in kidney brush-border membranes. Biochim Biophys Acta. 1991;1066:261-7 pubmed

More Information


  1. Beharry A, Sadovski O, Woolley G. Photo-control of peptide conformation on a timescale of seconds with a conformationally constrained, blue-absorbing, photo-switchable linker. Org Biomol Chem. 2008;6:4323-32 pubmed publisher
    ..This cross-linker design thus expands the possibilities for fast photo-control of peptide and protein structure in biochemical systems. ..
  2. Boivin D, Gingras D, Beliveau R. Purification and characterization of a membrane-bound protein carboxyl methyltransferase from rat kidney cortex. J Biol Chem. 1993;268:2610-5 pubmed
  3. Hollenstein M, Hipolito C, Lam C, Perrin D. A self-cleaving DNA enzyme modified with amines, guanidines and imidazoles operates independently of divalent metal cations (M2+). Nucleic Acids Res. 2009;37:1638-49 pubmed publisher
  4. Taurin S, Dulin N, Pchejetski D, Grygorczyk R, Tremblay J, Hamet P, et al. c-Fos expression in ouabain-treated vascular smooth muscle cells from rat aorta: evidence for an intracellular-sodium-mediated, calcium-independent mechanism. J Physiol. 2002;543:835-47 pubmed
  5. Lingwood C. Glycolipid receptors for verotoxin and Helicobacter pylori: role in pathology. Biochim Biophys Acta. 1999;1455:375-86 pubmed
  6. Diab C, Tribet C, Gohon Y, Popot J, Winnik F. Complexation of integral membrane proteins by phosphorylcholine-based amphipols. Biochim Biophys Acta. 2007;1768:2737-47 pubmed
    ..Results from isothermal titration calorimetry indicated that the energetics of the conversion of BR/detergent complexes into BR/amphipol complexes are similar for PC-amphipols and carboxylated amphiphols. ..
  7. Dai L, Friedman P, Quamme G. Acid-base changes alter Mg2+ uptake in mouse distal convoluted tubule cells. Am J Physiol. 1997;272:F759-66 pubmed
    ..0, -55 +/- 3%. Accordingly, MDCT cells become refractory to the stimulating effects of hyperpolarization at alkaline pH values. These studies show that protons may directly affect Mg2+ transport in MDCT cells. ..
  8. Arcand N, Kluepfel D, Paradis F, Morosoli R, Shareck F. Beta-mannanase of Streptomyces lividans 66: cloning and DNA sequence of the manA gene and characterization of the enzyme. Biochem J. 1993;290 ( Pt 3):857-63 pubmed
    ..of 205 i.u./mg of enzyme and a Km of 0.77 mg/ml. Data from SDS/PAGE and Western blotting show that the cloned enzyme was identical to that of the wild-type strain...
  9. Jane D, Morvay L, DaSilva L, Cavallo Medved D, Sloane B, Dufresne M. Cathepsin B localizes to plasma membrane caveolae of differentiating myoblasts and is secreted in an active form at physiological pH. Biol Chem. 2006;387:223-34 pubmed
    ..Collectively, these studies support an association of active cathepsin B with plasma membrane caveolae and the secretion of active cathepsin B from differentiating myoblasts during myoblast fusion. ..
  10. Liu G, Kulasingam V, Alexander R, Touret N, Fong A, Patel D, et al. Recycling of the membrane-anchored chemokine, CX3CL1. J Biol Chem. 2005;280:19858-66 pubmed
    ..The intracellular storage depot may serve as a source of the chemokine that could be rapidly mobilized by stimuli. ..
  11. Thomas J, Gilmour K. The impact of social status on the erythrocyte beta-adrenergic response in rainbow trout, Oncorhynchus mykiss. Comp Biochem Physiol A Mol Integr Physiol. 2006;143:162-72 pubmed
  12. Danyluk J, Houde M, Rassart E, Sarhan F. Differential expression of a gene encoding an acidic dehydrin in chilling sensitive and freezing tolerant gramineae species. FEBS Lett. 1994;344:20-4 pubmed
    ..Characterization of the regulatory factors controlling these genes may help to establish an appropriate strategy to improve freezing tolerance. ..
  13. Kishi T, Cloutier S, K√ľndig C, Deperthes D, Diamandis E. Activation and enzymatic characterization of recombinant human kallikrein 8. Biol Chem. 2006;387:723-31 pubmed
    ..Whereas Na+ had no effect on hK8 activity, Ni2+ and Zn2+ decreased the activity and Ca2+, Mg2+, and K+ had a stimulatory effect. Ca2+ was the best activator, with an optimal concentration of approximately 10 microM. ..
  14. Zhang J, Smith K, Tackaberry T, Sun X, Carpenter P, Slugoski M, et al. Characterization of the transport mechanism and permeant binding profile of the uridine permease Fui1p of Saccharomyces cerevisiae. J Biol Chem. 2006;281:28210-21 pubmed
    ..This characterization of Fui1p contributes to the emerging knowledge of the structure and function of the Fur family of permeases, including the Fui1p orthologs of pathogenic fungi. ..
  15. Hyduk S, Chan J, Duffy S, Chen M, Peterson M, Waddell T, et al. Phospholipase C, calcium, and calmodulin are critical for alpha4beta1 integrin affinity up-regulation and monocyte arrest triggered by chemoattractants. Blood. 2007;109:176-84 pubmed
    ..A critical role for PLC and calmodulin was also established for leukocyte arrest and adhesion strengthening. ..
  16. Alvarez B, Vithana E, Yang Z, Koh A, Yeung K, Yong V, et al. Identification and characterization of a novel mutation in the carbonic anhydrase IV gene that causes retinitis pigmentosa. Invest Ophthalmol Vis Sci. 2007;48:3459-68 pubmed
    ..This study indicates that, as with European patients with RP, mutations in CA4 also account for <or=1% of Chinese patients with RP. ..
  17. Alzahal O, Steele M, Valdes E, McBride B. Technical note: the use of a telemetric system to continuously monitor ruminal temperature and to predict ruminal pH in cattle. J Dairy Sci. 2009;92:5697-701 pubmed publisher
    ..Additional studies are needed to develop an algorithm that accounts for diet type, seasonal variation in temperature, and core body temperature to predict subacute ruminal acidosis effectively on farm. ..
  18. Assaad E, Mateescu M. The influence of protonation ratio on properties of carboxymethyl starch excipient at various substitution degrees: Structural insights and drug release kinetics. Int J Pharm. 2010;394:75-84 pubmed publisher
    ..The CMS(DS 0.20) was more sensitive to pH and showed an accelerated release rate in SIF. For the CMS formulations, a diffusion mechanism was suggested in SGF, whereas in SIF the release was mostly controlled by swelling and erosion...
  19. Paroutis P, Touret N, Grinstein S. The pH of the secretory pathway: measurement, determinants, and regulation. Physiology (Bethesda). 2004;19:207-15 pubmed
    ..The methods used for measurement of pH in the secretory pathway, its determinants, and its regulation are the subjects of this review. ..
  20. Xu Z, Love M, Ma L, Blum M, Bronskill P, Bernstein J, et al. Tryptophanyl-tRNA synthetase from Bacillus subtilis. Characterization and role of hydrophobicity in substrate recognition. J Biol Chem. 1989;264:4304-11 pubmed
  21. Summerscales J, Dawson J. Probing Dictyostelium severin structure and function by cross linking to actin. Biochem Cell Biol. 2004;82:343-50 pubmed
  22. Holowaty M, Sheng Y, Nguyen T, Arrowsmith C, Frappier L. Protein interaction domains of the ubiquitin-specific protease, USP7/HAUSP. J Biol Chem. 2003;278:47753-61 pubmed
  23. Ghitescu L, Gugliucci A, Dumas F. Actin and annexins I and II are among the main endothelial plasmalemma-associated proteins forming early glucose adducts in experimental diabetes. Diabetes. 2001;50:1666-74 pubmed
  24. Proudfoot M, Kuznetsova E, Brown G, Rao N, Kitagawa M, Mori H, et al. General enzymatic screens identify three new nucleotidases in Escherichia coli. Biochemical characterization of SurE, YfbR, and YjjG. J Biol Chem. 2004;279:54687-94 pubmed
    ..The differences in their sensitivities to nucleotides and their varied substrate specificities suggest that these enzymes play unique functions in the intracellular nucleotide metabolism in E. coli. ..
  25. Golovan S, Wang G, Zhang J, Forsberg C. Characterization and overproduction of the Escherichia coli appA encoded bifunctional enzyme that exhibits both phytase and acid phosphatase activities. Can J Microbiol. 2000;46:59-71 pubmed
    ..mL-1. The properties of the phytase including the low pH optimum, protease resistance, and high activity, demonstrates that the enzyme is a good candidate for industrial production as a feed enzyme. ..
  26. Jaswal J, Gandhi M, Finegan B, Dyck J, Clanachan A. Inhibition of p38 MAPK and AMPK restores adenosine-induced cardioprotection in hearts stressed by antecedent ischemia by altering glucose utilization. Am J Physiol Heart Circ Physiol. 2007;293:H1107-14 pubmed
    ..0.30 +/- 0.15, n = 8, P < 0.05). These data indicate that inhibition of p38 MAPK abolishes subsequent phosphorylation of AMPK and improves the coupling of glucose metabolism, thereby restoring adenosine-induced cardioprotection. ..
  27. Talpas C, Walz D, Lee L. 1H-NMR studies of bovine platelet factor 4: histidine assignments and interactions with heparin. Biochim Biophys Acta. 1991;1078:208-18 pubmed
  28. Vega A, Imoberdorf G, Keshmir M, Mohseni M. Novel composite photocatalyst made of TiO(2) nanoparticles. Water Sci Technol. 2010;61:903-9 pubmed publisher
    ..The photocatalytic activity was evaluated using model pesticide micro-pollutant. A complete model comprised of radiation, kinetic, and reactor bed models was also developed, allowing for detailed analysis of the photoreactor. ..
  29. Yamagata M, Tannock I. The chronic administration of drugs that inhibit the regulation of intracellular pH: in vitro and anti-tumour effects. Br J Cancer. 1996;73:1328-34 pubmed
    ..002 at tolerated doses of the four drugs used to treat KHT tumours. The experiments demonstrate the activity of drugs that inhibit the regulation of pHi against murine tumours when delivered by chronic infusion. ..
  30. Stoll V, Simpson S, Krauth Siegel R, Walsh C, Pai E. Glutathione reductase turned into trypanothione reductase: structural analysis of an engineered change in substrate specificity. Biochemistry. 1997;36:6437-47 pubmed
  31. Capyk J, D Angelo I, Strynadka N, Eltis L. Characterization of 3-ketosteroid 9{alpha}-hydroxylase, a Rieske oxygenase in the cholesterol degradation pathway of Mycobacterium tuberculosis. J Biol Chem. 2009;284:9937-46 pubmed publisher
    ..This study provides a solid basis for further studies of a key steroid-transforming enzyme of biotechnological and medical importance...
  32. Boivin D, Lin W, Beliveau R. Essential arginine residues in isoprenylcysteine protein carboxyl methyltransferase. Biochem Cell Biol. 1997;75:63-9 pubmed
    ..These results indicate that arginine residues are essential for the enzymatic activity of isoprenylcysteine carboxyl methyltransferase. ..
  33. Notenboom V, Birsan C, Nitz M, Rose D, Warren R, Withers S. Insights into transition state stabilization of the beta-1,4-glycosidase Cex by covalent intermediate accumulation in active site mutants. Nat Struct Biol. 1998;5:812-8 pubmed
  34. Frenette G, Sullivan R. Prostasome-like particles are involved in the transfer of P25b from the bovine epididymal fluid to the sperm surface. Mol Reprod Dev. 2001;59:115-21 pubmed
    ..P25b thus appears to be a marker of sperm epididymal maturation in bulls. ..
  35. Lei S, Orser B, Thatcher G, Reynolds J, MacDonald J. Positive allosteric modulators of AMPA receptors reduce proton-induced receptor desensitization in rat hippocampal neurons. J Neurophysiol. 2001;85:2030-8 pubmed
    ..These results demonstrate that a reduction in the proton sensitivity of receptor desensitization contributes to the mechanism of action of some positive modulators of AMPA receptors...
  36. Memari N, Jiang W, Diamandis E, Luo L. Enzymatic properties of human kallikrein-related peptidase 12 (KLK12). Biol Chem. 2007;388:427-35 pubmed
    ..Furthermore, we demonstrate that KLK12 is able to activate KLK11 zymogen in vitro. Our results indicate that KLK12 may participate in enzymatic cascades involving other kallikreins. ..
  37. Zou W, Cashman N. Acidic pH and detergents enhance in vitro conversion of human brain PrPC to a PrPSc-like form. J Biol Chem. 2002;277:43942-7 pubmed
    ..Further structural rearrangement seems essential for this species to acquire PK resistance, which can be promoted by the presence of a PrP(Sc) template. ..
  38. Zhang S, Kurata H, Kehl S, Fedida D. Rapid induction of P/C-type inactivation is the mechanism for acid-induced K+ current inhibition. J Gen Physiol. 2003;121:215-25 pubmed
    ..4, led us to favor the hypothesis that a reduction in the activation energy for the inactivation transition from the open state underlies the inhibition of hKv1.5 Na(+) current at low pH. ..
  39. Tarling C, He S, Sulzenbacher G, Bignon C, Bourne Y, Henrissat B, et al. Identification of the catalytic nucleophile of the family 29 alpha-L-fucosidase from Thermotoga maritima through trapping of a covalent glycosyl-enzyme intermediate and mutagenesis. J Biol Chem. 2003;278:47394-9 pubmed
    ..This article presents the first direct identification of the catalytic nucleophile of an alpha-L-fucosidase, a key step in the understanding of these important enzymes. ..
  40. Carpena X, Wiseman B, Deemagarn T, Singh R, Switala J, Ivancich A, et al. A molecular switch and electronic circuit modulate catalase activity in catalase-peroxidases. EMBO Rep. 2005;6:1156-62 pubmed publisher
  41. Chen L, Subirade M. Chitosan/beta-lactoglobulin core-shell nanoparticles as nutraceutical carriers. Biomaterials. 2005;26:6041-53 pubmed
    ..When transferred to simulated intestinal conditions, the (beta)lg shells of the nanoparticles were degraded by pancreatin. ..
  42. Pavlov E, Zakharian E, Bladen C, Diao C, Grimbly C, Reusch R, et al. A large, voltage-dependent channel, isolated from mitochondria by water-free chloroform extraction. Biophys J. 2005;88:2614-25 pubmed
    ..We suggest that the isolated complex may represent the ion-conducting module from the permeability transition pore. ..