Experts and Doctors on hela cells in Switzerland

Summary

Locale: Switzerland
Topic: hela cells

Top Publications

  1. Arning S, Grüter P, Bilbe G, Kramer A. Mammalian splicing factor SF1 is encoded by variant cDNAs and binds to RNA. RNA. 1996;2:794-810 pubmed
    ..The presence of this shared region in SF1 suggests functions in addition to its role in pre-spliceosome assembly. ..
  2. Akhmedov A, Lopez B. Human 100-kDa homologous DNA-pairing protein is the splicing factor PSF and promotes DNA strand invasion. Nucleic Acids Res. 2000;28:3022-30 pubmed
  3. Hofer T, Wenger R, Kramer M, Ferreira G, Gassmann M. Hypoxic up-regulation of erythroid 5-aminolevulinate synthase. Blood. 2003;101:348-50 pubmed
    ..Mutation of a previously predicted HIF-1-binding site (-323/-318) within this promoter fragment and DNA-binding assays revealed that hypoxic up-regulation is independent of this putative HIF-1 DNA-binding site. ..
  4. Zhang H, Kolb F, Brondani V, Billy E, Filipowicz W. Human Dicer preferentially cleaves dsRNAs at their termini without a requirement for ATP. EMBO J. 2002;21:5875-85 pubmed
    ..Additional experiments suggest that if ATP participates in the Dicer reaction in mammalian cells, it might be involved in product release needed for the multiple turnover of the enzyme. ..
  5. Michod D, Yang J, Chen J, Bonny C, Widmann C. A RasGAP-derived cell permeable peptide potently enhances genotoxin-induced cytotoxicity in tumor cells. Oncogene. 2004;23:8971-8 pubmed
    ..Our results demonstrate the feasibility in enhancing the efficacy of currently used drugs to selectively kill cancer cells using peptides derived from pro-apoptotic caspase substrate fragments. ..
  6. Cristofari G, Adolf E, Reichenbach P, Sikora K, Terns R, Terns M, et al. Human telomerase RNA accumulation in Cajal bodies facilitates telomerase recruitment to telomeres and telomere elongation. Mol Cell. 2007;27:882-9 pubmed
    ..Collectively, these data identify subnuclear localization as an important regulatory mechanism for telomere length homeostasis in human cells. ..
  7. Kotak S, Busso C, G nczy P. Cortical dynein is critical for proper spindle positioning in human cells. J Cell Biol. 2012;199:97-110 pubmed publisher
    ..Overall, we propose a model in which the ternary complex serves to anchor dynein at the plasma membrane to ensure correct spindle positioning...
  8. Sommer N, Junne T, Kalies K, Spiess M, Hartmann E. TRAP assists membrane protein topogenesis at the mammalian ER membrane. Biochim Biophys Acta. 2013;1833:3104-3111 pubmed publisher
    ..The results suggest a role of the TRAP complex in moderating the 'positive-inside' rule. ..
  9. Calado A, Treichel N, Müller E, Otto A, Kutay U. Exportin-5-mediated nuclear export of eukaryotic elongation factor 1A and tRNA. EMBO J. 2002;21:6216-24 pubmed
    ..Taken together, we show that there exists an alternative tRNA export pathway which can be exploited to keep eEF1A out of the cell nucleus. ..

More Information

Publications234 found, 100 shown here

  1. Renaud S, Bosman F, Benhattar J. Implication of the exon region in the regulation of the human telomerase reverse transcriptase gene promoter. Biochem Biophys Res Commun. 2003;300:47-54 pubmed
    ..These results provide the first evidence that the first two exons play a major role in the down-regulation of the hTERT promoter in telomerase-positive cells. ..
  2. Velluto D, Thomas S, Simeoni E, Swartz M, Hubbell J. PEG-b-PPS-b-PEI micelles and PEG-b-PPS/PEG-b-PPS-b-PEI mixed micelles as non-viral vectors for plasmid DNA: tumor immunotoxicity in B16F10 melanoma. Biomaterials. 2011;32:9839-47 pubmed publisher
  3. Da Cruz S, De Marchi U, Frieden M, Parone P, Martinou J, Demaurex N. SLP-2 negatively modulates mitochondrial sodium-calcium exchange. Cell Calcium. 2010;47:11-8 pubmed publisher
    ..These data show that SLP-2 modulates mitochondrial calcium extrusion, thereby altering the ability of mitochondria to buffer Ca(2+) and to shape cytosolic Ca(2+) signals. ..
  4. Roulet E, Armentero M, Krey G, Corthesy B, Dreyer C, Mermod N, et al. Regulation of the DNA-binding and transcriptional activities of Xenopus laevis NFI-X by a novel C-terminal domain. Mol Cell Biol. 1995;15:5552-62 pubmed
    ..Moreover, the region comprising the repressor domain participates in transactivation. Mechanisms that may allow the relief of DNA-binding inhibition in vivo and trigger transcriptional activation are discussed. ..
  5. Schreiber E, Tobler A, Malipiero U, Schaffner W, Fontana A. cDNA cloning of human N-Oct3, a nervous-system specific POU domain transcription factor binding to the octamer DNA motif. Nucleic Acids Res. 1993;21:253-8 pubmed
    ..In contrast to the putative N-Oct 5 proteins, which are transcriptionally inert, the N-Oct 3 protein activates transcription from a reporter gene promoter with an octamer sequence, when transiently expressed in HeLa cells. ..
  6. Scarselli M, Annibale P, Radenovic A. Cell type-specific ?2-adrenergic receptor clusters identified using photoactivated localization microscopy are not lipid raft related, but depend on actin cytoskeleton integrity. J Biol Chem. 2012;287:16768-80 pubmed publisher
  7. Kataoka T, Holler N, Micheau O, Martinon F, Tinel A, Hofmann K, et al. Bcl-rambo, a novel Bcl-2 homologue that induces apoptosis via its unique C-terminal extension. J Biol Chem. 2001;276:19548-54 pubmed
    ..Thus, Bcl-rambo constitutes a novel type of pro-apoptotic Bcl-2 member that triggers cell death independently of its BH motifs. ..
  8. Vulliémoz D, Roux L. "Rule of six": how does the Sendai virus RNA polymerase keep count?. J Virol. 2001;75:4506-18 pubmed
    ..The results strongly suggest that the application of the rule of six depends on the recognition of nucleotides positioned in the proper N phase context. ..
  9. Arora R, Brun C, Azzalin C. Transcription regulates telomere dynamics in human cancer cells. RNA. 2012;18:684-93 pubmed publisher
  10. Kramer A. Purification of splicing factor SF1, a heat-stable protein that functions in the assembly of a presplicing complex. Mol Cell Biol. 1992;12:4545-52 pubmed
    ..SF1 activity is completely resistant to temperatures of up to 100 degrees C. The purified protein does not appear to be associated with RNA-binding, RNA-annealing, or ATPase activity. ..
  11. Medcalf R, Ruegg M, Schleuning W. A DNA motif related to the cAMP-responsive element and an exon-located activator protein-2 binding site in the human tissue-type plasminogen activator gene promoter cooperate in basal expression and convey activation by phorbol ester and cAMP. J Biol Chem. 1990;265:14618-26 pubmed
    ..The results of band-shift analysis and competition titration experiments demonstrate that the CRE-like element acts as a low affinity binding site for the same proteins which recognize the authentic CRE. ..
  12. Gurcel L, Abrami L, Girardin S, Tschopp J, van der Goot F. Caspase-1 activation of lipid metabolic pathways in response to bacterial pore-forming toxins promotes cell survival. Cell. 2006;126:1135-45 pubmed
  13. Arquint C, Sonnen K, Stierhof Y, Nigg E. Cell-cycle-regulated expression of STIL controls centriole number in human cells. J Cell Sci. 2012;125:1342-52 pubmed publisher
    ..Thus, STIL cooperates with SAS-6 and PLK4 in the control of centriole number and represents a key centriole duplication factor in human cells. ..
  14. Müntener K, Zwicky R, Csucs G, Baici A. The alternative use of exons 2 and 3 in cathepsin B mRNA controls enzyme trafficking and triggers nuclear fragmentation in human cells. Histochem Cell Biol. 2003;119:93-101 pubmed
    ..The C-terminal, six-amino acid-long propeptide of cathepsin B did not exhibit a discernible role in protein trafficking. ..
  15. Kaufmann I, Martin G, Friedlein A, Langen H, Keller W. Human Fip1 is a subunit of CPSF that binds to U-rich RNA elements and stimulates poly(A) polymerase. EMBO J. 2004;23:616-26 pubmed
    ..These results show that hFip1 significantly contributes to CPSF-mediated stimulation of PAP activity. ..
  16. Tastanova A, Schulz A, Folcher M, Tolstrup A, Puklowski A, Kaufmann H, et al. Overexpression of YY1 increases the protein production in mammalian cells. J Biotechnol. 2016;219:72-85 pubmed publisher
  17. Kovacsovics M, Martinon F, Micheau O, Bodmer J, Hofmann K, Tschopp J. Overexpression of Helicard, a CARD-containing helicase cleaved during apoptosis, accelerates DNA degradation. Curr Biol. 2002;12:838-43 pubmed
    ..Helicard accelerates Fas ligand-mediated DNA degradation, whereas a noncleavable or a helicase-dead Helicard mutant does not, implicating Helicard in the nuclear remodeling occurring during apoptosis. ..
  18. Trotman L, Achermann D, Keller S, Straub M, Greber U. Non-classical export of an adenovirus structural protein. Traffic. 2003;4:390-402 pubmed
    ..Interestingly, the coexpression of Pb and fiber leads to both Pb and fiber export, termed 'protein abduction'. We suggest that fiber abduction might support viral dissemination in infected tissues by interfering with tissue integrity. ..
  19. Blenn C, Althaus F, Malanga M. Poly(ADP-ribose) glycohydrolase silencing protects against H2O2-induced cell death. Biochem J. 2006;396:419-29 pubmed
    ..No difference was observed in response to the DNA alkylating agent N-methyl-N'-nitro-N-nitrosoguanidine, suggesting a specific involvement of PARG in the cellular response to oxidative DNA damage. ..
  20. Kyburz A, Friedlein A, Langen H, Keller W. Direct interactions between subunits of CPSF and the U2 snRNP contribute to the coupling of pre-mRNA 3' end processing and splicing. Mol Cell. 2006;23:195-205 pubmed
    ..Moreover, we showed that efficient cleavage required the presence of the U2 snRNA in coupled assays. We therefore propose that the interaction between CPSF and the U2 snRNP contributes to the coupling of splicing and 3' end formation. ..
  21. Michalik L, Zoete V, Krey G, Grosdidier A, Gelman L, Chodanowski P, et al. Combined simulation and mutagenesis analyses reveal the involvement of key residues for peroxisome proliferator-activated receptor alpha helix 12 dynamic behavior. J Biol Chem. 2007;282:9666-77 pubmed
    ..Substitution of these residues suppresses PPARalpha constitutive activity, without changing PPARalpha ligand-dependent activation potential. ..
  22. Bourquin J, Stagljar I, Meier P, Moosmann P, Silke J, Baechi T, et al. A serine/arginine-rich nuclear matrix cyclophilin interacts with the C-terminal domain of RNA polymerase II. Nucleic Acids Res. 1997;25:2055-61 pubmed
  23. Cohausz O, Althaus F. Role of PARP-1 and PARP-2 in the expression of apoptosis-regulating genes in HeLa cells. Cell Biol Toxicol. 2009;25:379-91 pubmed publisher
    ..These findings suggest that PARP-1-but not PARP-2-is required for proper expression of major genes involved in regulation of apoptosis. ..
  24. O Connell M, Gerber A, Keller W. Purification of human double-stranded RNA-specific editase 1 (hRED1) involved in editing of brain glutamate receptor B pre-mRNA. J Biol Chem. 1997;272:473-8 pubmed
    ..This enzyme is distinct from double-stranded RNA-specific adenosine deaminase which we and others have previously purified and cloned. ..
  25. Perez F, Diamantopoulos G, Stalder R, Kreis T. CLIP-170 highlights growing microtubule ends in vivo. Cell. 1999;96:517-27 pubmed
    ..Drugs affecting microtubule dynamics rapidly inhibit movement of GFP-CLIP170 dashes. We propose that GFP-CLIP170 highlights growing microtubule ends by specifically recognizing the structure of a segment of newly polymerized tubulin. ..
  26. Matskevich A, Moelling K. Stimuli-dependent cleavage of Dicer during apoptosis. Biochem J. 2008;412:527-34 pubmed publisher
    ..Thus the apoptotic machinery may regulate the miRNA pathway by affecting individual proteins, such as Dicer. ..
  27. Wahle E, Martin G, Schiltz E, Keller W. Isolation and expression of cDNA clones encoding mammalian poly(A) polymerase. EMBO J. 1991;10:4251-7 pubmed
    ..3 kb. The sequence of the N-terminal half of bovine poly(A) polymerase is 47% identical with the amino acid sequence of the corresponding part of yeast poly(A) polymerase. Homologies to other proteins are of uncertain significance. ..
  28. Nyfeler B, Michnick S, Hauri H. Capturing protein interactions in the secretory pathway of living cells. Proc Natl Acad Sci U S A. 2005;102:6350-5 pubmed
    ..The study extends the application of PCA to carbohydrate-mediated protein-protein interactions of low affinity. ..
  29. Wyler E, Zimmermann M, Widmann B, Gstaiger M, Pfannstiel J, Kutay U, et al. Tandem affinity purification combined with inducible shRNA expression as a tool to study the maturation of macromolecular assemblies. RNA. 2011;17:189-200 pubmed publisher
    ..Thus, the combination of tandem affinity purification and shRNA induction provided further insights into late cytoplasmic 40S maturation steps, demonstrating the high potential of this method. ..
  30. Wurzenberger C, Held M, Lampson M, Poser I, Hyman A, Gerlich D. Sds22 and Repo-Man stabilize chromosome segregation by counteracting Aurora B on anaphase kinetochores. J Cell Biol. 2012;198:173-83 pubmed publisher
    ..Thus, our study identifies PP1-targeting subunits that regulate the microtubule-kinetochore interface during anaphase for faithful chromosome segregation...
  31. Jiang K, Rezabkova L, Hua S, Liu Q, Capitani G, Altelaar A, et al. Microtubule minus-end regulation at spindle poles by an ASPM-katanin complex. Nat Cell Biol. 2017;19:480-492 pubmed publisher
    ..We propose that the ASPM-katanin complex controls microtubule disassembly at spindle poles and that misregulation of this process can lead to microcephaly. ..
  32. Müller Immerglück M, Schaffner W, Matthias P. Transcription factor Oct-2A contains functionally redundant activating domains and works selectively from a promoter but not from a remote enhancer position in non-lymphoid (HeLa) cells. EMBO J. 1990;9:1625-34 pubmed
    ..From these experiments we consider it likely that the natural octamer factor variants can selectively activate the different naturally occurring octamer-containing promoters. ..
  33. Christofori G, Keller W. 3' cleavage and polyadenylation of mRNA precursors in vitro requires a poly(A) polymerase, a cleavage factor, and a snRNP. Cell. 1988;54:875-89 pubmed
    ..Further purification of CPF by buoyant density centrifugation, ion exchange, and affinity column chromatography or by gel filtration demonstrates that CPF activity resides in a ribonucleoprotein and copurifies with U11 snRNP. ..
  34. Pot C, Simonen M, Weinmann O, Schnell L, Christ F, Stoeckle S, et al. Nogo-A expressed in Schwann cells impairs axonal regeneration after peripheral nerve injury. J Cell Biol. 2002;159:29-35 pubmed
    ..Nogo-A thus overrides the growth-permissive and -promoting effects of the lesioned peripheral nerve, demonstrating its in vivo potency as an inhibitor of axonal regeneration. ..
  35. van der Vaart B, van Riel W, Doodhi H, Kevenaar J, Katrukha E, Gumy L, et al. CFEOM1-associated kinesin KIF21A is a cortical microtubule growth inhibitor. Dev Cell. 2013;27:145-160 pubmed publisher
    ..Our study provides mechanistic insight into cortical microtubule regulation and suggests that altered microtubule dynamics contribute to CFEOM1 pathogenesis. ..
  36. Sánchez Aparicio M, Garcin D, Rice C, Kolakofsky D, Garcia Sastre A, Baum A. Loss of Sendai virus C protein leads to accumulation of RIG-I immunostimulatory defective interfering RNA. J Gen Virol. 2017;98:1282-1293 pubmed publisher
    ..Our findings indicate that limiting DI genome production is an important function of viral IFN antagonist proteins. ..
  37. Domon C, Lorković Z, Valcarcel J, Filipowicz W. Multiple forms of the U2 small nuclear ribonucleoprotein auxiliary factor U2AF subunits expressed in higher plants. J Biol Chem. 1998;273:34603-10 pubmed
    ..The existence of multiple isoforms of U2AF may be quite general in plants because two genes expressing U2AF65 have been identified in Arabidopsis, and different isoforms of the U2AF small subunit are expressed in rice. ..
  38. Mayor T, Stierhof Y, Tanaka K, Fry A, Nigg E. The centrosomal protein C-Nap1 is required for cell cycle-regulated centrosome cohesion. J Cell Biol. 2000;151:837-46 pubmed
    ..We conclude that C-Nap1 is a key component of a dynamic, cell cycle-regulated structure that mediates centriole-centriole cohesion. ..
  39. Strnad P, Leidel S, Vinogradova T, Euteneuer U, Khodjakov A, GONCZY P. Regulated HsSAS-6 levels ensure formation of a single procentriole per centriole during the centrosome duplication cycle. Dev Cell. 2007;13:203-13 pubmed
  40. Puntener D, Engelke M, Ruzsics Z, Strunze S, Wilhelm C, Greber U. Stepwise loss of fluorescent core protein V from human adenovirus during entry into cells. J Virol. 2011;85:481-96 pubmed publisher
    ..Ad2-GFP-pV is genetically complete and hence enables detailed analyses of infection and spreading dynamics in cells and model organisms or assessment of oncolytic adenoviral potential...
  41. Prota A, Danel F, Bachmann F, Bargsten K, Buey R, Pohlmann J, et al. The novel microtubule-destabilizing drug BAL27862 binds to the colchicine site of tubulin with distinct effects on microtubule organization. J Mol Biol. 2014;426:1848-60 pubmed publisher
    ..Taken together, our data define BAL27862 as a potent, colchicine site-binding, microtubule-destabilizing agent with distinct effects on microtubule organization. ..
  42. Tassan J, Jaquenoud M, Fry A, Frutiger S, Hughes G, Nigg E. In vitro assembly of a functional human CDK7-cyclin H complex requires MAT1, a novel 36 kDa RING finger protein. EMBO J. 1995;14:5608-17 pubmed
    ..MAT1 constitutes the first example of an assembly factor that appears to be essential for the formation of an active CDK-cyclin complex. ..
  43. Hergovich A, Lisztwan J, Barry R, Ballschmieter P, Krek W. Regulation of microtubule stability by the von Hippel-Lindau tumour suppressor protein pVHL. Nat Cell Biol. 2003;5:64-70 pubmed
    ..Our data identify a role for pVHL in the regulation of microtubule dynamics and potentially provide a link between this function of pVHL and the pathogenesis of haemangioblastoma and phaeochromocytoma in the context of VHL disease. ..
  44. Li B, Zhuang L, Trueb B. Zyxin interacts with the SH3 domains of the cytoskeletal proteins LIM-nebulette and Lasp-1. J Biol Chem. 2004;279:20401-10 pubmed
    ..LIM-nebulette reveals a subcellular distribution at focal adhesions similar to Lasp-1. Thus, LIM-nebulette, Lasp-1, and zyxin may play an important role in the organization of focal adhesions. ..
  45. Pante N, Bastos R, McMorrow I, Burke B, Aebi U. Interactions and three-dimensional localization of a group of nuclear pore complex proteins. J Cell Biol. 1994;126:603-17 pubmed
    ..Labeling with these two antibodies has documented that NUP153 is a constituent of the nuclear basket with at least one of its epitopes residing in its terminal ring, whereas p250 is a constituent of the cytoplasmic filaments. ..
  46. Angehrn P, Buchmann S, Funk C, Goetschi E, Gmuender H, Hebeisen P, et al. New antibacterial agents derived from the DNA gyrase inhibitor cyclothialidine. J Med Chem. 2004;47:1487-513 pubmed
    ..By improving the pharmacokinetic properties of the most active compounds (94, 97), in particular by lowering their lipophilic properties, we were able to identify congeners of cyclothialidine (1) that showed efficacy in vivo. ..
  47. Geisler M, Blakeslee J, Bouchard R, Lee O, Vincenzetti V, Bandyopadhyay A, et al. Cellular efflux of auxin catalyzed by the Arabidopsis MDR/PGP transporter AtPGP1. Plant J. 2005;44:179-94 pubmed
  48. Picard D, Suslova E, Briand P. 2-color photobleaching experiments reveal distinct intracellular dynamics of two components of the Hsp90 complex. Exp Cell Res. 2006;312:3949-58 pubmed
    ..However, when Hsp90-p23 complexes are disrupted by the Hsp90 inhibitor geldanamycin, p23 moves by free diffusion while Hsp90 maintains its low mobility because it remains bound in remodeled multicomponent complexes. ..
  49. Amaro A, Samora C, Holtackers R, Wang E, Kingston I, Alonso M, et al. Molecular control of kinetochore-microtubule dynamics and chromosome oscillations. Nat Cell Biol. 2010;12:319-29 pubmed publisher
    ..We therefore propose that CENP-A NAC/CAD is a direct regulator of kinetochore-microtubule dynamics, which physically links centromeric DNA to microtubule plus ends. ..
  50. Escher C, Reiter L, Maclean B, Ossola R, Herzog F, Chilton J, et al. Using iRT, a normalized retention time for more targeted measurement of peptides. Proteomics. 2012;12:1111-21 pubmed publisher
    ..iRTs can be determined by any laboratory and shared transparently. The iRT concept has been implemented in Skyline, the most widely used software for MRM experiments. ..
  51. Haugstetter J, Blicher T, Ellgaard L. Identification and characterization of a novel thioredoxin-related transmembrane protein of the endoplasmic reticulum. J Biol Chem. 2005;280:8371-80 pubmed
    ..Interestingly, TMX3 showed oxidase activity, and in human tissue-culture cells the protein was found partially in the oxidized form, potentially suggesting a function of the protein as a dithiol oxidase. ..
  52. Mangeat B, Gers Huber G, Lehmann M, Zufferey M, Luban J, Piguet V. HIV-1 Vpu neutralizes the antiviral factor Tetherin/BST-2 by binding it and directing its beta-TrCP2-dependent degradation. PLoS Pathog. 2009;5:e1000574 pubmed publisher
    ..Identification of tetherin binding to Vpu provides a potential novel target for the development of drugs aimed at inhibiting HIV-1 replication. ..
  53. Wyss S, Berlioz Torrent C, Boge M, Blot G, Honing S, Benarous R, et al. The highly conserved C-terminal dileucine motif in the cytosolic domain of the human immunodeficiency virus type 1 envelope glycoprotein is critical for its association with the AP-1 clathrin adaptor [correction of adapter]. J Virol. 2001;75:2982-92 pubmed
    ..Finally, the C-terminal dileucine was shown to assist the membrane-proximal Y712 motif in restricting the cell surface expression of Env. ..
  54. Wendeler M, Paccaud J, Hauri H. Role of Sec24 isoforms in selective export of membrane proteins from the endoplasmic reticulum. EMBO Rep. 2007;8:258-64 pubmed
    ..Thus, human Sec24 isoforms expand the repertoire of cargo for signal-mediated ER export, but are in part functionally redundant. ..
  55. Rebsamen M, Meylan E, Curran J, Tschopp J. The antiviral adaptor proteins Cardif and Trif are processed and inactivated by caspases. Cell Death Differ. 2008;15:1804-11 pubmed publisher
    ..Importantly, poliovirus infection triggers a caspase-dependent cleavage of Cardif, suggesting that some viruses may activate caspases not only as a mean to facilitate shedding and replication, but also to impair antiviral responses. ..
  56. Mohanty S, Dal Molin M, Ganguli G, Padhi A, Jena P, Selchow P, et al. Mycobacterium tuberculosis EsxO (Rv2346c) promotes bacillary survival by inducing oxidative stress mediated genomic instability in macrophages. Tuberculosis (Edinb). 2016;96:44-57 pubmed publisher
    ..Thus EsxO plays an important role in mycobacterial pathogenesis in the context of innate immunity. ..
  57. Buhler M, Paillusson A, Mühlemann O. Efficient downregulation of immunoglobulin mu mRNA with premature translation-termination codons requires the 5'-half of the VDJ exon. Nucleic Acids Res. 2004;32:3304-15 pubmed
    ..Moreover, deletion of this NMD-promoting element from the Ig-micro minigene results in loss of strong NMD. ..
  58. Frouin I, Toueille M, Ferrari E, Shevelev I, Hubscher U. Phosphorylation of human DNA polymerase lambda by the cyclin-dependent kinase Cdk2/cyclin A complex is modulated by its association with proliferating cell nuclear antigen. Nucleic Acids Res. 2005;33:5354-61 pubmed
    ..Finally, Pol lambda is also phosphorylated in vivo in human cells and this phosphorylation is modulated during the cell cycle. ..
  59. Geisberger G, Gyenge E, Maake C, Patzke G. Trimethyl and carboxymethyl chitosan carriers for bio-active polymer-inorganic nanocomposites. Carbohydr Polym. 2013;91:58-67 pubmed publisher
    ..The anticancer activity of pristine {CoW(11)TiO(40)} and its TMC-nanocomposites was investigated in vitro with MTT assays and compared to a reference POM. ..
  60. Gaide O, Martinon F, Micheau O, Bonnet D, Thome M, Tschopp J. Carma1, a CARD-containing binding partner of Bcl10, induces Bcl10 phosphorylation and NF-kappaB activation. FEBS Lett. 2001;496:121-7 pubmed
    ..We propose that Carma1 is a crucial component of a novel Bcl10-dependent signaling pathway in T-cells that leads to the activation of NF-kappaB. ..
  61. Berthet C, Raj K, Saudan P, Beard P. How adeno-associated virus Rep78 protein arrests cells completely in S phase. Proc Natl Acad Sci U S A. 2005;102:13634-9 pubmed
  62. Sirena D, Lilienfeld B, Eisenhut M, Kälin S, Boucke K, Beerli R, et al. The human membrane cofactor CD46 is a receptor for species B adenovirus serotype 3. J Virol. 2004;78:4454-62 pubmed
    ..These results have implications for understanding viral pathogenesis and therapeutic gene delivery. ..
  63. Turgay Y, Champion L, Balázs C, Held M, Toso A, Gerlich D, et al. SUN proteins facilitate the removal of membranes from chromatin during nuclear envelope breakdown. J Cell Biol. 2014;204:1099-109 pubmed publisher
    ..Quantification of mitotic timing revealed a delay between NEBD and chromatin separation, indicating a role of SUN proteins in bipolar spindle assembly and mitotic progression. ..
  64. Didichenko S, Thelen M. Phosphatidylinositol 3-kinase c2alpha contains a nuclear localization sequence and associates with nuclear speckles. J Biol Chem. 2001;276:48135-42 pubmed
    ..The results suggest that phosphorylation of HsPI3K-C2alpha is inversely linked to mRNA transcription and supports the importance of phosphoinositides for nuclear activity. ..
  65. Lafont F, Tran van Nhieu G, Hanada K, Sansonetti P, van der Goot F. Initial steps of Shigella infection depend on the cholesterol/sphingolipid raft-mediated CD44-IpaB interaction. EMBO J. 2002;21:4449-57 pubmed
    ..Our results show that rafts are implicated in Shigella binding and entry, suggesting that raft-associated molecular machineries are engaged in mediating the cell signalling response required for the invasion process. ..
  66. Caspers P, Bury L, Gaucher B, Heim J, Shapiro S, Siegrist S, et al. In vitro and in vivo properties of dihydrophthalazine antifolates, a novel family of antibacterial drugs. Antimicrob Agents Chemother. 2009;53:3620-7 pubmed publisher
    ..6 to 6.25 mg/kg. ..
  67. Nyfeler B, Reiterer V, Wendeler M, Stefan E, Zhang B, Michnick S, et al. Identification of ERGIC-53 as an intracellular transport receptor of alpha1-antitrypsin. J Cell Biol. 2008;180:705-12 pubmed publisher
    ..The results reveal ERGIC-53 to be an intracellular transport receptor of alpha1-AT and provide direct evidence for active receptor-mediated ER export of a soluble secretory protein in higher eukaryotes. ..
  68. Silvan U, Boiteux C, Sütterlin R, Schroeder U, Mannherz H, Jockusch B, et al. An antiparallel actin dimer is associated with the endocytic pathway in mammalian cells. J Struct Biol. 2012;177:70-80 pubmed publisher
    ..Taken together, our results strongly support a functional role of LD in the patterning of supramolecular actin assemblies in mammalian cells. ..
  69. Gstaiger M, Knoepfel L, Georgiev O, Schaffner W, Hovens C. A B-cell coactivator of octamer-binding transcription factors. Nature. 1995;373:360-2 pubmed
    ..We conclude that Bob1 could represent a new tissue-specific transcriptional coactivator which may convert a ubiquitously expressed transcription factor to a cell-type-specific activator. ..
  70. Wenger R, Rolfs A, Marti H, Guenet J, Gassmann M. Nucleotide sequence, chromosomal assignment and mRNA expression of mouse hypoxia-inducible factor-1 alpha. Biochem Biophys Res Commun. 1996;223:54-9 pubmed
    ..Despite a strong erythropoietin induction, HIF-1 alpha mRNA concentrations were not upregulated in hypoxic mouse tissues. ..
  71. Shen W, Frieden M, Demaurex N. Local cytosolic Ca2+ elevations are required for stromal interaction molecule 1 (STIM1) de-oligomerization and termination of store-operated Ca2+ entry. J Biol Chem. 2011;286:36448-59 pubmed publisher
    ..These data indicate that the refilling of ER Ca(2+) stores is not sufficient to induce STIM1 de-oligomerization and that localized Ca(2+) elevations in the vicinity of assembled SOCE complexes are required for the termination of SOCE. ..
  72. Udayar V, Buggia Prévot V, Guerreiro R, Siegel G, Rambabu N, Soohoo A, et al. A paired RNAi and RabGAP overexpression screen identifies Rab11 as a regulator of β-amyloid production. Cell Rep. 2013;5:1536-51 pubmed publisher
    ..Our results reveal trafficking pathways that regulate Aβ levels and show how systems biology approaches can unravel the molecular complexity underlying AD. ..
  73. O Connell M, Krause S, Higuchi M, Hsuan J, Totty N, Jenny A, et al. Cloning of cDNAs encoding mammalian double-stranded RNA-specific adenosine deaminase. Mol Cell Biol. 1995;15:1389-97 pubmed
    ..Staining of HeLa cells confirms the nuclear localization of the dsRNA-specific adenosine deaminase. In situ hybridization in rat brain slices indicates a widespread distribution of the enzyme in the brain. ..
  74. Kelleher C, Kurth I, Lingner J. Human protection of telomeres 1 (POT1) is a negative regulator of telomerase activity in vitro. Mol Cell Biol. 2005;25:808-18 pubmed
    ..Furthermore, POT1 is incapable of inhibiting telomeric repeat addition to substrate primers that are defective for POT1 binding, suggesting that in vivo, POT1 likely affects substrate access to telomerase. ..
  75. Micheau O, Lens S, Gaide O, Alevizopoulos K, Tschopp J. NF-kappaB signals induce the expression of c-FLIP. Mol Cell Biol. 2001;21:5299-305 pubmed
    ..This treatment leads to the rapid downregulation of FLIP but not to that of TRAF2. Our findings suggest that FLIP is an important mediator of NF-kappaB-controlled antiapoptotic signals. ..
  76. Balamurugan K, Egli D, Selvaraj A, Zhang B, Georgiev O, Schaffner W. Metal-responsive transcription factor (MTF-1) and heavy metal stress response in Drosophila and mammalian cells: a functional comparison. Biol Chem. 2004;385:597-603 pubmed
  77. Zinzalla V, Stracka D, Oppliger W, Hall M. Activation of mTORC2 by association with the ribosome. Cell. 2011;144:757-68 pubmed publisher
    ..As ribosome content determines growth capacity of a cell, this mechanism of TORC2 regulation ensures that TORC2 is active only in growing cells. ..
  78. Aeby E, Ullu E, Yepiskoposyan H, Schimanski B, Roditi I, Mühlemann O, et al. tRNASec is transcribed by RNA polymerase II in Trypanosoma brucei but not in humans. Nucleic Acids Res. 2010;38:5833-43 pubmed publisher
    ..Moreover, intron-encoded tRNAs are present in a number of eukaryotic genomes indicating that Pol-II transcription of tRNAs may not be restricted to trypanosomatids. ..
  79. Can D, Spingler B, Schmutz P, Mendes F, Raposinho P, Fernandes C, et al. [(Cp-R)M(CO)3] (M=Re or 99mTc) Arylsulfonamide, arylsulfamide, and arylsulfamate conjugates for selective targeting of human carbonic anhydrase IX. Angew Chem Int Ed Engl. 2012;51:3354-7 pubmed publisher
    ..The syntheses and in vitro evaluation of [(Cp-R)M(CO)(3)] (Cp=cyclopentadienyl, M=Re, (99m)Tc) with R being a highly potent carbonic-anhydrase-targeting vector is reported. ..
  80. Kisielow M, Kleiner S, Nagasawa M, Faisal A, Nagamine Y. Isoform-specific knockdown and expression of adaptor protein ShcA using small interfering RNA. Biochem J. 2002;363:1-5 pubmed
    ..This allowed functional analysis of individual ShcA isoforms and may be more generally applicable for studying genes encoding multiple proteins. ..
  81. Gasser G, Pinto A, Neumann S, Sosniak A, Seitz M, Merz K, et al. Synthesis, characterisation and bioimaging of a fluorescent rhenium-containing PNA bioconjugate. Dalton Trans. 2012;41:2304-13 pubmed publisher
    ..Furthermore, Re-PNA could be detected in living cells using fluorescent microscopy...
  82. Cathomen T, Naim H, Cattaneo R. Measles viruses with altered envelope protein cytoplasmic tails gain cell fusion competence. J Virol. 1998;72:1224-34 pubmed
    ..We conclude that in MV infections, including SSPE, the glycoprotein tails are involved not only in virus envelope assembly but also in the control of virus-induced cell fusion. ..
  83. Betz C, Stracka D, Prescianotto Baschong C, Frieden M, Demaurex N, Hall M. Feature Article: mTOR complex 2-Akt signaling at mitochondria-associated endoplasmic reticulum membranes (MAM) regulates mitochondrial physiology. Proc Natl Acad Sci U S A. 2013;110:12526-34 pubmed publisher
    ..Thus, mTORC2 is at the core of a MAM signaling hub that controls growth and metabolism. ..
  84. Didichenko S, Fragoso C, Thelen M. Mitotic and stress-induced phosphorylation of HsPI3K-C2alpha targets the protein for degradation. J Biol Chem. 2003;278:26055-64 pubmed
    ..Phosphorylation appears to be a prerequisite for proteasome-dependent degradation of HsPIK3-C2alpha and may therefore contribute indirectly to the regulation of the activity of the kinase. ..
  85. Breuza L, Halbeisen R, Jeno P, Otte S, Barlowe C, Hong W, et al. Proteomics of endoplasmic reticulum-Golgi intermediate compartment (ERGIC) membranes from brefeldin A-treated HepG2 cells identifies ERGIC-32, a new cycling protein that interacts with human Erv46. J Biol Chem. 2004;279:47242-53 pubmed
    ..Our novel approach for the isolation of the ERGIC from BFA-treated cells may ultimately lead to the identification of all proteins rapidly cycling early in the secretory pathway. ..
  86. Schegg B, Hülsmeier A, Rutschmann C, Maag C, Hennet T. Core glycosylation of collagen is initiated by two beta(1-O)galactosyltransferases. Mol Cell Biol. 2009;29:943-52 pubmed publisher
    ..The GLT25D1 and GLT25D2 genes now allow addressing of the biological significance of collagen glycosylation and the importance of this posttranslational modification in the etiology of connective tissue disorders. ..
  87. Coley S, Lavi E, Sawicki S, Fu L, Schelle B, Karl N, et al. Recombinant mouse hepatitis virus strain A59 from cloned, full-length cDNA replicates to high titers in vitro and is fully pathogenic in vivo. J Virol. 2005;79:3097-106 pubmed
  88. van Loon B, Hubscher U. An 8-oxo-guanine repair pathway coordinated by MUTYH glycosylase and DNA polymerase lambda. Proc Natl Acad Sci U S A. 2009;106:18201-6 pubmed publisher
    ..These results reveal a cellular response pathway to ROS, important to sustain genomic stability and modulate carcinogenesis. ..
  89. Barraud P, Banerjee S, Mohamed W, Jantsch M, Allain F. A bimodular nuclear localization signal assembled via an extended double-stranded RNA-binding domain acts as an RNA-sensing signal for transportin 1. Proc Natl Acad Sci U S A. 2014;111:E1852-61 pubmed publisher
    ..We also provide a structural model showing how Trn1 can recognize the dsRBD-NLS and how dsRNA binding can interfere with Trn1 binding. ..
  90. Fettelschoss V, Burda P, Sagné C, Coelho D, De Laet C, Lutz S, et al. Clinical or ATPase domain mutations in ABCD4 disrupt the interaction between the vitamin B12-trafficking proteins ABCD4 and LMBD1. J Biol Chem. 2017;292:11980-11991 pubmed publisher
  91. Vollenweider F, Kappeler F, Itin C, Hauri H. Mistargeting of the lectin ERGIC-53 to the endoplasmic reticulum of HeLa cells impairs the secretion of a lysosomal enzyme. J Cell Biol. 1998;142:377-89 pubmed
    ..The results suggest that the recycling of ERGIC-53 is required for efficient intracellular transport of a small subset of glycoproteins, but it does not appear to be essential for the majority of glycoproteins. ..
  92. Blangy A, Lane H, d Hérin P, Harper M, Kress M, Nigg E. Phosphorylation by p34cdc2 regulates spindle association of human Eg5, a kinesin-related motor essential for bipolar spindle formation in vivo. Cell. 1995;83:1159-69 pubmed
    ..These results indicate that HsEg5 is required for establishing a bipolar spindle and that p34cdc2 protein kinase directly regulates its localization. ..