Genomes and Genes
Experts and Doctors on hela cells in Germany
Topic: hela cells
Publications346 found, 100 shown here
- Arndt V, Daniel C, Nastainczyk W, Alberti S, Hohfeld J. BAG-2 acts as an inhibitor of the chaperone-associated ubiquitin ligase CHIP. Mol Biol Cell. 2005;16:5891-900 pubmed..The presented data therefore establish multiple mechanisms to control the destructive activity of the CHIP ubiquitin ligase in human cells. ..
- Kim C, Slavinskaya Z, Merrill A, Kaufmann S. Human alpha-defensins neutralize toxins of the mono-ADP-ribosyltransferase family. Biochem J. 2006;399:225-9 pubmed..Our results reveal that toxin neutralization represents a novel biological function of HNPs in host defence. ..
- Uhlmann T, Boeing S, Lehmbacher M, Meisterernst M. The VP16 activation domain establishes an active mediator lacking CDK8 in vivo. J Biol Chem. 2007;282:2163-73 pubmed..We provide in vitro and in vivo evidence that VP16 activates transcription through a specific MED25-associated Mediator, which is deficient in CDK8. ..
- Sichtig N, Körfer N, Steger G. Papillomavirus binding factor binds to SAP30 and represses transcription via recruitment of the HDAC1 co-repressor complex. Arch Biochem Biophys. 2007;467:67-75 pubmed..The addition of Trichostatin A, an inhibitor of HDACs, alleviated PBF-mediated repression. Thus, PBF-mediated repression of transcription involves specific DNA-binding and the recruitment of the SIN3A-HDAC1 complex. ..
- Kittler R, Pelletier L, Heninger A, Slabicki M, Theis M, Miroslaw L, et al. Genome-scale RNAi profiling of cell division in human tissue culture cells. Nat Cell Biol. 2007;9:1401-12 pubmed..Our work provides an experimental framework from which the systematic analysis of novel genes necessary for cell division in human cells can begin. ..
- Kirkin V, Lamark T, Johansen T, Dikic I. NBR1 cooperates with p62 in selective autophagy of ubiquitinated targets. Autophagy. 2009;5:732-3 pubmed..We propose that NBR1 together with p62 promotes autophagic degradation of ubiquitinated targets and simultaneously regulates their aggregation when autophagy becomes limited. ..
- Tochowicz A, Goettig P, Evans R, Visse R, Shitomi Y, Palmisano R, et al. The dimer interface of the membrane type 1 matrix metalloproteinase hemopexin domain: crystal structure and biological functions. J Biol Chem. 2011;286:7587-600 pubmed publisher..These results shed light on the structural basis of MT1-MMP dimerization that is crucial to promote cellular invasion. ..
- Ebstein F, Lehmann A, Kloetzel P. The FAT10- and ubiquitin-dependent degradation machineries exhibit common and distinct requirements for MHC class I antigen presentation. Cell Mol Life Sci. 2012;69:2443-54 pubmed publisher..Collectively, our data establish FAT10 modification as a distinct and alternative signal for facilitated MHC class I antigen presentation. ..
- Nagel J, Gross B, Meggendorfer M, Preiss C, Grez M, Brack Werner R, et al. Stably integrated and expressed retroviral sequences can influence nuclear location and chromatin condensation of the integration locus. Chromosoma. 2012;121:353-67 pubmed publisher..We thus present an example where a few kb of exogenous DNA are sufficient to significantly alter the large-scale chromatin organization of an endogenous locus. ..
- Preußner M, Wilhelmi I, Schultz A, Finkernagel F, Michel M, Moroy T, et al. Rhythmic U2af26 alternative splicing controls PERIOD1 stability and the circadian clock in mice. Mol Cell. 2014;54:651-62 pubmed publisher..These data suggest light-induced U2af26 alternative splicing to be a buffering mechanism that limits PERIOD1 induction, thus stabilizing the circadian clock against abnormal changes in light:dark conditions. ..
- Frazier M, Davies A, Voehler M, Kendall A, Borner G, Chazin W, et al. Molecular Basis for the Interaction Between AP4 Î²4 and its Accessory Protein, Tepsin. Traffic. 2016;17:400-15 pubmed publisher..These data provide one of the first detailed mechanistic glimpses at AP4 coat assembly and should provide an entry point for probing the role of AP4-coated vesicles in cell biology, and especially in neuronal function. ..
- Rubie C, Kölsch K, Halajda B, Eichler H, Wagenpfeil S, Roemer K, et al. microRNA-496 - A new, potentially aging-relevant regulator of mTOR. Cell Cycle. 2016;15:1108-16 pubmed publisher..Together, these findings point to the possibility that miR-496 is involved in the regulation of human aging through the control of mTOR. ..
- Blomberg I, Hoffmann I. Ectopic expression of Cdc25A accelerates the G(1)/S transition and leads to premature activation of cyclin E- and cyclin A-dependent kinases. Mol Cell Biol. 1999;19:6183-94 pubmed..These results suggest that Cdc25A is an important regulator of the G(1)/S-phase transition and that cyclin E- and cyclin A-dependent kinases act as direct targets. ..
- Rottner K, Krause M, Gimona M, Small J, Wehland J. Zyxin is not colocalized with vasodilator-stimulated phosphoprotein (VASP) at lamellipodial tips and exhibits different dynamics to vinculin, paxillin, and VASP in focal adhesions. Mol Biol Cell. 2001;12:3103-13 pubmed..Together, these data identify zyxin as an early target for signals leading to adhesion disassembly, but exclude its role in recruiting Ena/VASP proteins to the tips of lamellipodia and filopodia. ..
- Boll M, Foltz M, Rubio Aliaga I, Kottra G, Daniel H. Functional characterization of two novel mammalian electrogenic proton-dependent amino acid cotransporters. J Biol Chem. 2002;277:22966-73 pubmed..Phenotypically, the PAT1 transporter possesses the same functional characteristics as the previously described proton-dependent amino acid transport process in apical membranes of intestinal and renal epithelial cells. ..
- Hartlieb B, Modrof J, M hlberger E, Klenk H, Becker S. Oligomerization of Ebola virus VP30 is essential for viral transcription and can be inhibited by a synthetic peptide. J Biol Chem. 2003;278:41830-6 pubmed publisher..When E30pep-wt was transfected into EBOV-infected cells, the peptide inhibited viral replication suggesting that inhibition of VP30 oligomerization represents a target for EBOV antiviral drugs...
- Grether Beck S, Felsner I, Brenden H, Krutmann J. Mitochondrial cytochrome c release mediates ceramide-induced activator protein 2 activation and gene expression in keratinocytes. J Biol Chem. 2003;278:47498-507 pubmed..Mitochondria thus have a previously unrecognized function in signaling ceramide-induced transcription factor activation and gene regulation. ..
- Bullwinkel J, Baron Luhr B, Lüdemann A, Wohlenberg C, Gerdes J, Scholzen T. Ki-67 protein is associated with ribosomal RNA transcription in quiescent and proliferating cells. J Cell Physiol. 2006;206:624-35 pubmed..These data strongly suggest a role for pKi-67 in the early steps of rRNA synthesis. ..
- Duwel M, Ungewickell E. Clathrin-dependent association of CVAK104 with endosomes and the trans-Golgi network. Mol Biol Cell. 2006;17:4513-25 pubmed..Together, our data suggest a function for CVAK104 in clathrin-dependent pathways between the trans-Golgi network and the endosomal system. ..
- Garvalov B, Zuber B, Bouchet Marquis C, Kudryashev M, Gruska M, Beck M, et al. Luminal particles within cellular microtubules. J Cell Biol. 2006;174:759-65 pubmed..A higher accumulation of particles was seen near the retracting plus ends of microtubules. The luminal particles were abundant in neurons, but were also observed in other cells, such as astrocytes and stem cells. ..
- Otter S, Grimmler M, Neuenkirchen N, Chari A, Sickmann A, Fischer U. A comprehensive interaction map of the human survival of motor neuron (SMN) complex. J Biol Chem. 2007;282:5825-33 pubmed..Collectively, the data presented here provide a basis for the detailed mechanistic and structural analysis of the assembly machinery of U snRNPs. ..
- Guggenberger C, Ilgen D, Adamski J. Functional analysis of cholesterol biosynthesis by RNA interference. J Steroid Biochem Mol Biol. 2007;104:105-9 pubmed..These stable transfected cell lines represent novel tools for the analysis of cholesterol biosynthesis. ..
- Hasskarl J, Mern D, Munger K. Interference of the dominant negative helix-loop-helix protein ID1 with the proteasomal subunit S5A causes centrosomal abnormalities. Oncogene. 2008;27:1657-64 pubmed..These results are consistent with the model that ID1 dysregulates centrosome homeostasis at least in part by interfering with S5A activities at the centrosome. ..
- Waldecker M, Kautenburger T, Daumann H, Busch C, Schrenk D. Inhibition of histone-deacetylase activity by short-chain fatty acids and some polyphenol metabolites formed in the colon. J Nutr Biochem. 2008;19:587-93 pubmed
- Kleinschmidt M, Streubel G, Samans B, Krause M, Bauer U. The protein arginine methyltransferases CARM1 and PRMT1 cooperate in gene regulation. Nucleic Acids Res. 2008;36:3202-13 pubmed publisher..Our data demonstrate a widespread cooperation of CARM1 and PRMT1 in gene activation as well as repression and that STAT5-dependent transcription of the CITED2 gene is a novel pathway coactivated by the two methyltransferases. ..
- Maier T, Tausch L, Hoernig M, Coste O, Schmidt R, Angioni C, et al. Celecoxib inhibits 5-lipoxygenase. Biochem Pharmacol. 2008;76:862-72 pubmed publisher..2 mg/kg. Together, celecoxib is a direct inhibitor of 5-LO in vitro and in vivo. These findings provide a potential molecular basis for some of the described COX-2-independent pharmacological effects of celecoxib. ..
- Happel N, Stoldt S, Schmidt B, Doenecke D. M phase-specific phosphorylation of histone H1.5 at threonine 10 by GSK-3. J Mol Biol. 2009;386:339-50 pubmed publisher..Furthermore, addition of specific glycogen synthase kinase-3 inhibitors led to a reduction in phosphorylation at this site both in vivo and in vitro. ..
- Gaitanos T, Santamaria A, Jeyaprakash A, Wang B, Conti E, Nigg E. Stable kinetochore-microtubule interactions depend on the Ska complex and its new component Ska3/C13Orf3. EMBO J. 2009;28:1442-52 pubmed publisher..We propose that the Ska complex functionally complements the KMN, providing an additional layer of stability to KT-MT attachment and possibly signalling completion of attachment to the spindle checkpoint. ..
- Muller B, Anders M, Akiyama H, Welsch S, Glass B, Nikovics K, et al. HIV-1 Gag processing intermediates trans-dominantly interfere with HIV-1 infectivity. J Biol Chem. 2009;284:29692-703 pubmed publisher..These effects are likely to be important for the strong activity of PI at concentrations achieved in vivo and also bear relevance for the mechanism of action of the antiviral drug bevirimat. ..
- Malik R, Lenobel R, Santamaria A, Ries A, Nigg E, Körner R. Quantitative analysis of the human spindle phosphoproteome at distinct mitotic stages. J Proteome Res. 2009;8:4553-63 pubmed publisher..Taken together, our results constitute a large quantitative data resource of phosphorylation abundances at distinct mitotic stages and they provide insight into the systems properties of phosphorylation dynamics during mitosis. ..
- Schmidt T, Striebinger H, Haas J, Bailer S. The heterogeneous nuclear ribonucleoprotein K is important for Herpes simplex virus-1 propagation. FEBS Lett. 2010;584:4361-5 pubmed publisher..Our results indicate that hnRNP K has an impact on a late step of herpesviral propagation making it a potential antiviral target. ..
- Leiprecht N, Munoz C, Alesutan I, Siraskar G, Sopjani M, F ller M, et al. Regulation of Na(+)-coupled glucose carrier SGLT1 by human papillomavirus 18 E6 protein. Biochem Biophys Res Commun. 2011;404:695-700 pubmed publisher..Accrodingly, E6 was not effective by increasing carrier protein stability in the membrane. In conclusion, HPV18 E6 oncoprotein participates in the upregulation of SGLT1...
- Schubert T, Pusch M, Diermeier S, Benes V, Kremmer E, Imhof A, et al. Df31 protein and snoRNAs maintain accessible higher-order structures of chromatin. Mol Cell. 2012;48:434-44 pubmed publisher..Analysis of caRNAs in human cells also reveals a strong enrichment of snoRNAs, implying a conserved role for these molecules in higher-order structures of chromatin. ..
- Casanova C, Sehr P, Putzker K, Hentze M, Neumann B, Duncan K, et al. Automated high-throughput RNAi screening in human cells combined with reporter mRNA transfection to identify novel regulators of translation. PLoS ONE. 2012;7:e45943 pubmed publisher..Our automated method is scalable and readily adapted for use with other mRNA regulatory elements. Consequently, it should be a generally useful approach for high-throughput identification of novel regulators of mRNA translation. ..
- Ritter A, Sanhaji M, Friemel A, Roth S, Rolle U, Louwen F, et al. Functional analysis of phosphorylation of the mitotic centromere-associated kinesin by Aurora B kinase in human tumor cells. Cell Cycle. 2015;14:3755-67 pubmed publisher..These data provide the first molecular explanation for clinical observation, where an overexpression of MCAK was associated with lymphatic invasion and lymph node metastasis in gastric and colorectal cancer patients. ..
- Zink L, Delbarre E, Eberl H, Keilhauer E, Bönisch C, Pünzeler S, et al. H3.Y discriminates between HIRA and DAXX chaperone complexes and reveals unexpected insights into human DAXX-H3.3-H4 binding and deposition requirements. Nucleic Acids Res. 2017;45:5691-5706 pubmed publisher..These data demonstrate that H3.Y's unique amino acids allow a functional distinction between HIRA and DAXX binding and its consequent deposition into open chromatin. ..
- Vukotic M, Nolte H, König T, Saita S, Ananjew M, Kruger M, et al. Acylglycerol Kinase Mutated in Sengers Syndrome Is a Subunit of the TIM22 Protein Translocase in Mitochondria. Mol Cell. 2017;67:471-483.e7 pubmed publisher..The dual function of AGK as lipid kinase and constituent of the TIM22 complex reveals that disturbances in both phospholipid metabolism and mitochondrial protein biogenesis contribute to the pathogenesis of Sengers syndrome. ..
- Umeda A, Meyerholz A, Ungewickell E. Identification of the universal cofactor (auxilin 2) in clathrin coat dissociation. Eur J Cell Biol. 2000;79:336-42 pubmed..The cellular distribution of membrane-associated adaptors was unaffected by overexpression of GAK. Our results point to a hsc70/auxilin-based uncoating system as a ubiquitous feature of eukaryotic cells...
- Hofmann W, Reichart B, Ewald A, Muller E, Schmitt I, Stauber R, et al. Cofactor requirements for nuclear export of Rev response element (RRE)- and constitutive transport element (CTE)-containing retroviral RNAs. An unexpected role for actin. J Cell Biol. 2001;152:895-910 pubmed..In summary, our data provide evidence that actin plays an important functional role in nuclear export not only of retroviral RNAs but also of host proteins such as protein kinase inhibitor (PKI)...
- Zobiack N, Rescher U, Laarmann S, Michgehl S, Schmidt M, Gerke V. Cell-surface attachment of pedestal-forming enteropathogenic E. coli induces a clustering of raft components and a recruitment of annexin 2. J Cell Sci. 2002;115:91-8 pubmed
- Kreppel F, Biermann V, Kochanek S, Schiedner G. A DNA-based method to assay total and infectious particle contents and helper virus contamination in high-capacity adenoviral vector preparations. Hum Gene Ther. 2002;13:1151-6 pubmed..This assay is a useful tool for determining bioactivity data of adenoviral vector preparations and, importantly, allows their comparison among different studies. ..
- Zander K, Sherman M, Tessmer U, Bruns K, Wray V, Prechtel A, et al. Cyclophilin A interacts with HIV-1 Vpr and is required for its functional expression. J Biol Chem. 2003;278:43202-13 pubmed
- Czyz J, Madeja Z, Irmer U, Korohoda W, Hülser D. Flavonoid apigenin inhibits motility and invasiveness of carcinoma cells in vitro. Int J Cancer. 2005;114:12-8 pubmed..This inhibitory effect of apigenin on tumour cell invasiveness in vitro demonstrates that apigenin may exert its anti-tumorigenic effect in vivo via inhibition of tumour cell penetration of the healthy tissue. ..
- Quensel C, Friedrich B, Sommer T, Hartmann E, Kohler M. In vivo analysis of importin alpha proteins reveals cellular proliferation inhibition and substrate specificity. Mol Cell Biol. 2004;24:10246-55 pubmed..Thus, importin alpha3 is the only alpha importin responsible for the classical nuclear import of RCC1 in living cells. ..
- Andrei M, Ingelfinger D, Heintzmann R, Achsel T, Rivera Pomar R, Luhrmann R. A role for eIF4E and eIF4E-transporter in targeting mRNPs to mammalian processing bodies. RNA. 2005;11:717-27 pubmed..These data support a model in which mRNPs undergo several successive steps of remodeling and/or 3' trimming until their composition or structural organization promotes their accumulation in P bodies. ..
- Neumeier M, Weigert J, Schaffler A, Weiss T, Kirchner S, Laberer S, et al. Regulation of adiponectin receptor 1 in human hepatocytes by agonists of nuclear receptors. Biochem Biophys Res Commun. 2005;334:924-9 pubmed..Elevated AdipoR1 induced by hepatotoxic troglitazone may indicate a role of this receptor in adiponectin-mediated beneficial effects in liver damage. ..
- de Oliveira S, Hoffmeister M, Gambaryan S, Muller Esterl W, Guimaraes J, Smolenski A. Phosphodiesterase 2A forms a complex with the co-chaperone XAP2 and regulates nuclear translocation of the aryl hydrocarbon receptor. J Biol Chem. 2007;282:13656-63 pubmed..We conclude that XAP2 targets PDE2A to the AhR complex, thereby restricting AhR mobility, possibly by a local reduction of cAMP levels. Our results provide first insights into the elusive cAMP-dependent regulation of AhR. ..
- Huang B, Schiefer J, Sass C, Landwehrmeyer G, Kosinski C, Kochanek S. High-capacity adenoviral vector-mediated reduction of huntingtin aggregate load in vitro and in vivo. Hum Gene Ther. 2007;18:303-11 pubmed..In both models an efficient reduction in mutant Htt aggregate load measured by decreased Htt-IR aggregate formation was observed. Our results support the further development of shRNA for HD therapy. ..
- Zahn C, Jaschke A, Weiske J, Hommel A, Hesse D, Augustin R, et al. ADP-ribosylation factor-like GTPase ARFRP1 is required for trans-Golgi to plasma membrane trafficking of E-cadherin. J Biol Chem. 2008;283:27179-88 pubmed publisher..These data indicate that knock-out of Arfrp1 disrupts the trafficking of E-cadherin through the Golgi and suggest an essential role of the GTPase in trans-Golgi network function. ..
- Harder N, Mora Bermúdez F, Godinez W, Wünsche A, Eils R, Ellenberg J, et al. Automatic analysis of dividing cells in live cell movies to detect mitotic delays and correlate phenotypes in time. Genome Res. 2009;19:2113-24 pubmed publisher..Our approach can be readily applied to comparable assays aiming at uncovering the dynamic cause of cell division phenotypes. ..
- Pillich H, Loose M, Zimmer K, Chakraborty T. Activation of the unfolded protein response by Listeria monocytogenes. Cell Microbiol. 2012;14:949-64 pubmed publisher..Induction of ER stress by thapsigargin or tunicamycin reduced intracellular bacterial number. Our findings suggest that UPR plays an important role in the cell autonomous defence responses to bacterial infection. ..
- Werner A, Disanza A, Reifenberger N, Habeck G, Becker J, Calabrese M, et al. SCFFbxw5 mediates transient degradation of actin remodeller Eps8 to allow proper mitotic progression. Nat Cell Biol. 2013;15:179-88 pubmed publisher..Our findings identify SCF(Fbxw5)-driven fluctuation of Eps8 levels as an important mechanism that contributes to cell-shape changes during entry into-and exit from-mitosis. ..
- Das S, Lindemann C, Young B, Müller J, Österreich B, Ternette N, et al. Natural mutations in a Staphylococcus aureus virulence regulator attenuate cytotoxicity but permit bacteremia and abscess formation. Proc Natl Acad Sci U S A. 2016;113:E3101-10 pubmed publisher..aureus bloodstream infections. Such a phenotype could promote deep infection with limited early clinical manifestations, raising concerns that bacterial evolution within the human body may contribute to severe infection. ..
- Erkel G, Rether J, Anke T, Sterner O. S14-95, a novel inhibitor of the JAK/STAT pathway from a Penicillium species. J Antibiot (Tokyo). 2003;56:337-43 pubmed..In addition, S14-95 inhibited the activation of the p38 MAP kinase, which is involved in the inducible expression of many proinflammatory genes. ..
- Heltweg B, Dequiedt F, Verdin E, Jung M. Nonisotopic substrate for assaying both human zinc and NAD+-dependent histone deacetylases. Anal Biochem. 2003;319:42-8 pubmed..Interestingly, MAL shows some relative preference toward class II, indicating that subtype selectivity in small-molecule HDAC substrates might be obtained. ..
- Corona T, Bao Q, Christ N, Schwartz T, Li J, Droge P. Activation of site-specific DNA integration in human cells by a single chain integration host factor. Nucleic Acids Res. 2003;31:5140-8 pubmed..Hence, scIHF2 may be used as a novel regulatory cofactor for recombination or other DNA transactions in mammalian cells that require or benefit from sequence-specific high precision DNA bending. ..
- Wichert S, Fokianos K, Strimmer K. Identifying periodically expressed transcripts in microarray time series data. Bioinformatics. 2004;20:5-20 pubmed..The approach has been implemented in the R package GeneTS available from http://www.stat.uni-muenchen.de/~strimmer/software.html under the terms of the GNU General Public License. ..
- Thoma C, Bergamini G, Galy B, Hundsdoerfer P, Hentze M. Enhancement of IRES-mediated translation of the c-myc and BiP mRNAs by the poly(A) tail is independent of intact eIF4G and PABP. Mol Cell. 2004;15:925-35 pubmed..The polyadenylated mRNA translation from cellular IRESs is also profoundly sensitive to eIF4A activity in vitro. These mechanistic and molecular distinctions implicate the potential for a new layer of translational control mechanisms. ..
- Klein U, Haindl M, Nigg E, Muller S. RanBP2 and SENP3 function in a mitotic SUMO2/3 conjugation-deconjugation cycle on Borealin. Mol Biol Cell. 2009;20:410-8 pubmed publisher..These data thus delineate a mitotic SUMO2/3 conjugation-deconjugation cycle of Borealin and further assign a regulatory function of RanBP2 and SENP3 in the mitotic SUMO pathway. ..
- Fossum E, Friedel C, Rajagopala S, Titz B, Baiker A, Schmidt T, et al. Evolutionarily conserved herpesviral protein interaction networks. PLoS Pathog. 2009;5:e1000570 pubmed publisher..By combining interactomes of different species we were able to systematically address the low coverage of the Y2H system and to extract biologically relevant interactions which were not evident from single species...
- Wilkens V, Kohl W, Busch K. Restricted diffusion of OXPHOS complexes in dynamic mitochondria delays their exchange between cristae and engenders a transitory mosaic distribution. J Cell Sci. 2013;126:103-16 pubmed publisher..The resulting transitory mosaic composition of the inner mitochondrial membrane illuminates mitochondrial heterogeneity and potentially is linked to local differences in function and membrane potential. ..
- Fabrizio P, Laggerbauer B, Lauber J, Lane W, Luhrmann R. An evolutionarily conserved U5 snRNP-specific protein is a GTP-binding factor closely related to the ribosomal translocase EF-2. EMBO J. 1997;16:4092-106 pubmed..This is to date the first evidence that a G domain-containing protein plays an essential role in the pre-mRNA splicing process. ..
- Jäkel S, Gorlich D. Importin beta, transportin, RanBP5 and RanBP7 mediate nuclear import of ribosomal proteins in mammalian cells. EMBO J. 1998;17:4491-502 pubmed..The presence of distinct binding sites for rpL23a and the M9 import signal in transportin, and for rpL23a and importin alpha in importin beta might explain how a single receptor can recognize very different import signals. ..
- Weide T, Bayer M, Koster M, Siebrasse J, Peters R, Barnekow A. The Golgi matrix protein GM130: a specific interacting partner of the small GTPase rab1b. EMBO Rep. 2001;2:336-41 pubmed..We mapped the rab1b binding site of GM130 and provide evidence that it is different to the previously described p115 and Grasp65 binding sites of the GM130 protein. ..
- Kübler D. Ecto-protein kinase substrate p120 revealed as the cell-surface-expressed nucleolar phosphoprotein Nopp140: a candidate protein for extracellular Ca2+-sensing. Biochem J. 2001;360:579-87 pubmed..Our results indicate that, in addition to nucleolin, a further nucleolar protein, which was considered initially to be strictly intracellular, is identified as a cell-surface phosphoprotein. ..
- Kufer T, Sillje H, Körner R, Gruss O, Meraldi P, Nigg E. Human TPX2 is required for targeting Aurora-A kinase to the spindle. J Cell Biol. 2002;158:617-23 pubmed..We propose that human TPX2 is required for targeting Aurora-A kinase to the spindle apparatus. In turn, Aurora-A might regulate the function of TPX2 during spindle assembly. ..
- Fischer L, Szellas D, Radmark O, Steinhilber D, Werz O. Phosphorylation- and stimulus-dependent inhibition of cellular 5-lipoxygenase activity by nonredox-type inhibitors. FASEB J. 2003;17:949-51 pubmed
- Bradl H, Wittmann J, Milius D, Vettermann C, Jack H. Interaction of murine precursor B cell receptor with stroma cells is controlled by the unique tail of lambda 5 and stroma cell-associated heparan sulfate. J Immunol. 2003;171:2338-48 pubmed
- Storch S, Pohl S, Braulke T. A dileucine motif and a cluster of acidic amino acids in the second cytoplasmic domain of the batten disease-related CLN3 protein are required for efficient lysosomal targeting. J Biol Chem. 2004;279:53625-34 pubmed
- Mitsdoerffer M, Schreiner B, Kieseier B, Neuhaus O, Dichgans J, Hartung H, et al. Monocyte-derived HLA-G acts as a strong inhibitor of autologous CD4 T cell activation and is upregulated by interferon-beta in vitro and in vivo: rationale for the therapy of multiple sclerosis. J Neuroimmunol. 2005;159:155-64 pubmed..We conclude that some desirable immune-modulatory effects of INF-beta might be accomplished via the upregulation of the immune-tolerogenic molecule HLA-G. ..
- Boehmann Y, Enterlein S, Randolf A, M hlberger E. A reconstituted replication and transcription system for Ebola virus Reston and comparison with Ebola virus Zaire. Virology. 2005;332:406-17 pubmed publisher..Although these two proteins did not constitute an active polymerase complex, it was shown by immunofluorescence analysis that they were still able to interact...
- Werk D, Schubert S, Lindig V, Grunert H, Zeichhardt H, Erdmann V, et al. Developing an effective RNA interference strategy against a plus-strand RNA virus: silencing of coxsackievirus B3 and its cognate coxsackievirus-adenovirus receptor. Biol Chem. 2005;386:857-63 pubmed..Furthermore, we have first evidence that inhibition of the cellular coxsackievirus-adenovirus receptor (CAR) by RNAi also reduces the virus titre. Our strategy is likely to be applicable to other (RNA) viruses as well. ..
- Patre M, Tabbert A, Hermann D, Walczak H, Rackwitz H, Cordes V, et al. Caspases target only two architectural components within the core structure of the nuclear pore complex. J Biol Chem. 2006;281:1296-304 pubmed..Caspase-mediated proteolysis of only few nuclear pore complex components may exemplify a general strategy of apoptotic cells to efficiently disable huge macromolecular machines. ..
- Prechtel A, Chemnitz J, Schirmer S, Ehlers C, Langbein Detsch I, Stülke J, et al. Expression of CD83 is regulated by HuR via a novel cis-active coding region RNA element. J Biol Chem. 2006;281:10912-25 pubmed..In summary, the data presented demonstrate that the HuR-CRM1 axis affects the nucleocytoplasmic translocation of CD83 mRNA under regular physiological conditions. ..
- Herrmann G, Kais S, Hoffbauer J, Shah Hosseini K, Brüggenolte N, Schober H, et al. Conserved interactions of the splicing factor Ntr1/Spp382 with proteins involved in DNA double-strand break repair and telomere metabolism. Nucleic Acids Res. 2007;35:2321-32 pubmed..Like PinX1, NTR1 localizes to telomeres and associates with nucleoli in yeast and human cells, suggesting a function in localized control of DSBR. ..
- Vermeulen M, Mulder K, Denissov S, Pijnappel W, van Schaik F, Varier R, et al. Selective anchoring of TFIID to nucleosomes by trimethylation of histone H3 lysine 4. Cell. 2007;131:58-69 pubmed..Our experiments reveal crosstalk between histone modifications and the transcription factor TFIID. This has important implications for regulation of RNA polymerase II-mediated transcription in higher eukaryotes. ..
- Mathew R, Hartmuth K, Möhlmann S, Urlaub H, Ficner R, Luhrmann R. Phosphorylation of human PRP28 by SRPK2 is required for integration of the U4/U6-U5 tri-snRNP into the spliceosome. Nat Struct Mol Biol. 2008;15:435-43 pubmed publisher..Our results demonstrate a role for SRPK2 in splicing and reveal a previously unknown function for PRP28 in spliceosome assembly. ..
- Sonntag S, Sohl G, Dobrowolski R, Zhang J, Theis M, Winterhager E, et al. Mouse lens connexin23 (Gje1) does not form functional gap junction channels but causes enhanced ATP release from HeLa cells. Eur J Cell Biol. 2009;88:65-77 pubmed publisher..Therefore, Cx23 seems to share functional properties with pannexin (hemi) channels rather than gap junction channels of other connexins. ..
- Becker M, Stolz A, Ertych N, Bastians H. Centromere localization of INCENP-Aurora B is sufficient to support spindle checkpoint function. Cell Cycle. 2010;9:1360-72 pubmed..These results indicate that INCENP-Aurora B localized at centromeres/inner kinetochores is sufficient to mediate SAC activity upon spindle disruption. ..
- Schneider M, Spoden G, Florin L, Lambert C. Identification of the dynein light chains required for human papillomavirus infection. Cell Microbiol. 2011;13:32-46 pubmed publisher..Therefore, this work identified two host cell proteins involved in HPV16 infection that are most likely required for transport purposes towards the nucleus...
- Herrmann F, Wink M. Synergistic interactions of saponins and monoterpenes in HeLa cells, Cos7 cells and in erythrocytes. Phytomedicine. 2011;18:1191-6 pubmed publisher..The synergistic effect of combinations of saponins with monoterpenes opens a complete new field of possible applications in medicine to overcome resistance in multidrug resistant microbial and human cell...
- Baarlink C, Wang H, Grosse R. Nuclear actin network assembly by formins regulates the SRF coactivator MAL. Science. 2013;340:864-7 pubmed publisher..Activated mDia promoted rapid and reversible nuclear actin network assembly, subsequent MAL nuclear accumulation, and SRF activity. Thus, a dynamic polymeric actin structure within the nucleus is part of the serum response. ..
- Altenburg A, Abdel Naser M, Nikolakis G, Wild T, Wojtalewicz N. CD40/CD40 ligand interactions and TNF? treatment reduce activity of P105 promoter of the human papilloma virus-18 in vitro. Exp Oncol. 2016;38:22-5 pubmed..01 and p < 0.001, respectively). It is likely that the CD40/CD40L interactions and TNF? are effective against cervical carcinomas by repressing transcriptional activity of HPV promoter. This can result in new adjuvant treatments. ..
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