Experts and Doctors on green fluorescent proteins in United Kingdom


Locale: United Kingdom
Topic: green fluorescent proteins

Top Publications

  1. Lipinski D, Yusuf M, Barnard A, Damant C, Charbel Issa P, Singh M, et al. Characterization of a dominant cone degeneration in a green fluorescent protein-reporter mouse with disruption of Loci associated with human dominant retinal dystrophy. Invest Ophthalmol Vis Sci. 2011;52:6617-23 pubmed publisher
    ..The mechanism underlying the degeneration remains elusive; however, disruption of loci associated with dominantly inherited retinal degenerations in humans makes this mouse of great interest. ..
  2. Lee J, Urban S, Garvey C, Freeman M. Regulated intracellular ligand transport and proteolysis control EGF signal activation in Drosophila. Cell. 2001;107:161-71 pubmed
    ..Rhomboid-1 is localized in the Golgi, where it promotes the cleavage of Spitz. This defines a novel growth factor release mechanism that is distinct from metalloprotease-dependent shedding from the cell surface. ..
  3. Tolmachova T, Anders R, Stinchcombe J, Bossi G, Griffiths G, Huxley C, et al. A general role for Rab27a in secretory cells. Mol Biol Cell. 2004;15:332-44 pubmed
    ..The present strategy represents one way in which the complex pattern of expression and function of proteins involved in specialized cell types may be unraveled. ..
  4. Warburton E, Glover C, Massey P, Wan H, Johnson B, Bienemann A, et al. cAMP responsive element-binding protein phosphorylation is necessary for perirhinal long-term potentiation and recognition memory. J Neurosci. 2005;25:6296-303 pubmed
  5. Schmierer B, Hill C. Kinetic analysis of Smad nucleocytoplasmic shuttling reveals a mechanism for transforming growth factor beta-dependent nuclear accumulation of Smads. Mol Cell Biol. 2005;25:9845-58 pubmed
    ..The proposed mechanism reconciles signal-dependent nuclear accumulation of Smad2 with its continuous nucleocytoplasmic cycling properties. ..
  6. Poggi L, Vitorino M, Masai I, Harris W. Influences on neural lineage and mode of division in the zebrafish retina in vivo. J Cell Biol. 2005;171:991-9 pubmed
    ..This study provides the first insight into reproducible lineage patterns of retinal progenitors in vivo and the first evidence that environmental signals influence the orientation of cell division and the lineage of neural progenitors. ..
  7. Millo H, Bownes M. The expression pattern and cellular localisation of Myosin VI during the Drosophila melanogaster life cycle. Gene Expr Patterns. 2007;7:501-10 pubmed
  8. Batut J, Howell M, Hill C. Kinesin-mediated transport of Smad2 is required for signaling in response to TGF-beta ligands. Dev Cell. 2007;12:261-74 pubmed
    ..Our results reveal that kinesin-mediated transport of Smad2 along microtubules to the receptors is an essential step in ligand-induced Smad2 activation. ..
  9. Bardet P, Kolahgar G, Mynett A, Miguel Aliaga I, Briscoe J, Meier P, et al. A fluorescent reporter of caspase activity for live imaging. Proc Natl Acad Sci U S A. 2008;105:13901-5 pubmed publisher
    ..As expected from the high conservation of caspases, it is also cleaved in dying cells of chick embryos. It is therefore likely to be generally useful to track the spatiotemporal pattern of caspase activity in a variety of species. ..

More Information

Publications172 found, 100 shown here

  1. Serrels A, Timpson P, Canel M, Schwarz J, Carragher N, Frame M, et al. Real-time study of E-cadherin and membrane dynamics in living animals: implications for disease modeling and drug development. Cancer Res. 2009;69:2714-9 pubmed publisher
    ..Furthermore, this work highlights critical differences in molecular dynamics in vitro and in vivo, which have important implications for the use of cultured disease models as surrogates for living tissue. ..
  2. Shi X, van Mierlo J, French A, Elliott R. Visualizing the replication cycle of bunyamwera orthobunyavirus expressing fluorescent protein-tagged Gc glycoprotein. J Virol. 2010;84:8460-9 pubmed publisher
    ..The fluorescent protein-tagged viruses will be valuable reagents for live-cell imaging studies to investigate virus entry, budding, and morphogenesis in real time. ..
  3. Jeyasekharan A, Ayoub N, Mahen R, Ries J, Esposito A, Rajendra E, et al. DNA damage regulates the mobility of Brca2 within the nucleoplasm of living cells. Proc Natl Acad Sci U S A. 2010;107:21937-42 pubmed publisher
    ..Our findings identify signal-regulated changes in nucleoplasmic protein diffusion as a means to control biochemical reactions in the cell nucleus. ..
  4. Crabtree M, Brixey R, Batchelor H, Hale A, Channon K. Integrated redox sensor and effector functions for tetrahydrobiopterin- and glutathionylation-dependent endothelial nitric-oxide synthase uncoupling. J Biol Chem. 2013;288:561-9 pubmed publisher
  5. Lin C, White R, Sparkes I, Ashwin P. Modeling Endoplasmic Reticulum Network Maintenance in a Plant Cell. Biophys J. 2017;113:214-222 pubmed publisher
    ..This model produces quantitatively similar ER networks to those observed in experiments. We use the model to explore the effect of parameter variation on statistical properties of the ER network. ..
  6. Zernicka Goetz M, Pines J. Use of Green Fluorescent Protein in mouse embryos. Methods. 2001;24:55-60 pubmed
    ..In this article we outline the ways in which we have used GFP mRNA and cDNA in our studies of mouse cell lineages, and to characterize the behavior of proteins within the embryos. ..
  7. Gusterson R, Jazrawi E, Adcock I, Latchman D. The transcriptional co-activators CREB-binding protein (CBP) and p300 play a critical role in cardiac hypertrophy that is dependent on their histone acetyltransferase activity. J Biol Chem. 2003;278:6838-47 pubmed
    ..This is the first time a role for these factors, and their HAT activity, in hypertrophy has been directly demonstrated. ..
  8. Tsuboi T, McMahon H, Rutter G. Mechanisms of dense core vesicle recapture following "kiss and run" ("cavicapture") exocytosis in insulin-secreting cells. J Biol Chem. 2004;279:47115-24 pubmed
  9. Parsons M, Monypenny J, Ameer Beg S, Millard T, Machesky L, Peter M, et al. Spatially distinct binding of Cdc42 to PAK1 and N-WASP in breast carcinoma cells. Mol Cell Biol. 2005;25:1680-95 pubmed
    ..These data reveal for the first time distinct spatial association patterns between Cdc42 and its key effector proteins controlling cytoskeletal remodeling. ..
  10. Martin S, Tatham M, Hay R, Samuel I. Quantitative analysis of multi-protein interactions using FRET: application to the SUMO pathway. Protein Sci. 2008;17:777-84 pubmed publisher
    ..These assays provide powerful tools for the study of competitive biochemical cascades and the extent to which drug candidates modify protein interactions. ..
  11. Esposito E, Keatings S, Gardner K, Harris J, Riis E, McConnell G. Confocal laser scanning microscopy using a frequency doubled vertical external cavity surface emitting laser. Rev Sci Instrum. 2008;79:083702 pubmed publisher
    ..Imaging of fluorescein and eGFP labeled biological samples using the attenuated solid-state source provided high-resolution images at lower cost and with improved reliability...
  12. Sheridan J, Taoudi S, Medvinsky A, Blackburn C. A novel method for the generation of reaggregated organotypic cultures that permits juxtaposition of defined cell populations. Genesis. 2009;47:346-51 pubmed publisher
    ..Additionally, compaction reaggregation permits the juxtaposition of different cell layers within the aggregated structure thus providing the means to study inductive interactions between different cell populations in vitro. ..
  13. Koroleva O, Calder G, Pendle A, Kim S, Lewandowska D, Simpson C, et al. Dynamic behavior of Arabidopsis eIF4A-III, putative core protein of exon junction complex: fast relocation to nucleolus and splicing speckles under hypoxia. Plant Cell. 2009;21:1592-606 pubmed publisher
    ..Sequestration of eIF4A-III into nuclear pools with different mobility is likely to reflect the transcriptional and mRNA processing state of the cell. ..
  14. Jiang J, Cole D, Westwood N, Macpherson L, Farzaneh F, Mufti G, et al. Crucial roles for protein kinase C isoforms in tumor-specific killing by apoptin. Cancer Res. 2010;70:7242-52 pubmed publisher
    ..Further, they highlight the potential of apoptin and its cellular regulators to purge bone marrow used in autologous transplantation for multiple myeloma. ..
  15. Bird J, Daudet N, Warchol M, Gale J. Supporting cells eliminate dying sensory hair cells to maintain epithelial integrity in the avian inner ear. J Neurosci. 2010;30:12545-56 pubmed publisher
    ..Our data show that supporting cells not only maintain epithelial integrity during trauma but suggest they may also be an integral part of the hair cell death process itself...
  16. Lancaster O, Breuer M, Cullen C, Ito T, Ohkura H. The meiotic recombination checkpoint suppresses NHK-1 kinase to prevent reorganisation of the oocyte nucleus in Drosophila. PLoS Genet. 2010;6:e1001179 pubmed publisher
    ..Therefore we propose that NHK-1 is a crucial regulator of meiosis and that the meiotic checkpoint suppresses NHK-1 activity to prevent oocyte nuclear reorganisation until DNA breaks are repaired. ..
  17. Du M, Wang G, Ismail T, Gross S, Fernig D, Barraclough R, et al. S100P dissociates myosin IIA filaments and focal adhesion sites to reduce cell adhesion and enhance cell migration. J Biol Chem. 2012;287:15330-44 pubmed publisher
  18. Lu Y, Schneider H, Rudd C. Murine regulatory T cells differ from conventional T cells in resisting the CTLA-4 reversal of TCR stop-signal. Blood. 2012;120:4560-70 pubmed publisher
    ..Our findings identify for the first time a mechanistic explanation to account for CTLA-4-negative regulation of Tconv cells but not Tregs in immune responses. ..
  19. MartĂ­ M, Stancombe M, Webb A. Cell- and stimulus type-specific intracellular free Ca2+ signals in Arabidopsis. Plant Physiol. 2013;163:625-34 pubmed publisher
  20. Schmidt E, Hvam M, Antunes F, Cameron J, Viuff D, Andersen B, et al. Direct demonstration of a neonatal Fc receptor (FcRn)-driven endosomal sorting pathway for cellular recycling of albumin. J Biol Chem. 2017;292:13312-13322 pubmed publisher
  21. Whitley P, Reaves B, Hashimoto M, Riley A, Potter B, Holman G. Identification of mammalian Vps24p as an effector of phosphatidylinositol 3,5-bisphosphate-dependent endosome compartmentalization. J Biol Chem. 2003;278:38786-95 pubmed publisher
  22. Sharon M, Witt S, Felderer K, Rockel B, Baumeister W, Robinson C. 20S proteasomes have the potential to keep substrates in store for continual degradation. J Biol Chem. 2006;281:9569-75 pubmed
  23. Yap M, Nisole S, Stoye J. A single amino acid change in the SPRY domain of human Trim5alpha leads to HIV-1 restriction. Curr Biol. 2005;15:73-8 pubmed
    ..A single amino acid substitution (R332P) in the human Trim5alpha can confer the ability to restrict HIV-1, suggesting that small changes during evolution may have profound effects on our susceptibility to cross-species infection. ..
  24. Cheung G, Jupp O, Cousin M. Activity-dependent bulk endocytosis and clathrin-dependent endocytosis replenish specific synaptic vesicle pools in central nerve terminals. J Neurosci. 2010;30:8151-61 pubmed publisher
    ..The differential replenishment of specific SV pools by independent SV retrieval modes illustrates how previously experienced neuronal activity impacts the capability of central nerve terminals to respond to future stimuli. ..
  25. Segonzac C, NIMCHUK Z, Beck M, Tarr P, Robatzek S, Meyerowitz E, et al. The shoot apical meristem regulatory peptide CLV3 does not activate innate immunity. Plant Cell. 2012;24:3186-92 pubmed
    ..Here, we report experimental evidence demonstrating that FLS2 does not recognize CLV3 and that the shoot apical meristem is immune to bacteria independently of CLV3 perception. ..
  26. Strutt H, Strutt D. EGF signaling and ommatidial rotation in the Drosophila eye. Curr Biol. 2003;13:1451-7 pubmed
    ..Secondly, reducing EGF signaling can specifically affect ommatidial rotation without showing transformation of the mystery cells or defects in polarity protein localization. ..
  27. Ashby M, Maier S, Nishimune A, Henley J. Lateral diffusion drives constitutive exchange of AMPA receptors at dendritic spines and is regulated by spine morphology. J Neurosci. 2006;26:7046-55 pubmed
  28. Strutt H, Strutt D. Differential stability of flamingo protein complexes underlies the establishment of planar polarity. Curr Biol. 2008;18:1555-64 pubmed publisher
  29. Chotard C, Leung W, Salecker I. glial cells missing and gcm2 cell autonomously regulate both glial and neuronal development in the visual system of Drosophila. Neuron. 2005;48:237-51 pubmed
    ..Our findings further suggest that gcm genes regulate neurogenesis through collaboration with the Hedgehog-signaling pathway. ..
  30. Strahl H, Hamoen L. Membrane potential is important for bacterial cell division. Proc Natl Acad Sci U S A. 2010;107:12281-6 pubmed publisher
    ..This function of the membrane potential has implications for how these morphogenetic proteins work and provide an explanation for the effects observed with certain antimicrobial compounds. ..
  31. Nakamura Y, Morrow D, Modgil A, Huyghe D, Deeb T, Lumb M, et al. Proteomic Characterization of Inhibitory Synapses Using a Novel pHluorin-tagged ?-Aminobutyric Acid Receptor, Type A (GABAA), ?2 Subunit Knock-in Mouse. J Biol Chem. 2016;291:12394-407 pubmed publisher
    ..Thus, our experiments illustrate the complexity of the GABAAR proteome and enhance our understanding of the mechanisms neurons use to construct inhibitory synapses. ..
  32. Lane J, Lucocq J, Pryde J, Barr F, Woodman P, Allan V, et al. Caspase-mediated cleavage of the stacking protein GRASP65 is required for Golgi fragmentation during apoptosis. J Cell Biol. 2002;156:495-509 pubmed
    ..Our results suggest that GRASP65 is an important structural component required for maintenance of Golgi apparatus integrity. ..
  33. Wykes R, Bauer C, Khan S, Weiss J, Seward E. Differential regulation of endogenous N- and P/Q-type Ca2+ channel inactivation by Ca2+/calmodulin impacts on their ability to support exocytosis in chromaffin cells. J Neurosci. 2007;27:5236-48 pubmed
  34. Roca M, Kuo H, Lichius A, Freitag M, Read N. Nuclear dynamics, mitosis, and the cytoskeleton during the early stages of colony initiation in Neurospora crassa. Eukaryot Cell. 2010;9:1171-83 pubmed publisher
    ..Latrunculin B treatment, imaging of F-actin in living cells using Lifeact-red fluorescent protein (RFP), and analysis of mutants defective in the Arp2/3 complex demonstrated that actin plays important roles in CAT fusion...
  35. Andrews P, Ovechkina Y, Morrice N, Wagenbach M, Duncan K, Wordeman L, et al. Aurora B regulates MCAK at the mitotic centromere. Dev Cell. 2004;6:253-68 pubmed
    ..These results link Aurora B activity to MCAK function, with Aurora B regulating MCAK's activity and its localization at the centromere and kinetochore...
  36. Zhang X, Santuccione A, Leung C, Marino S. Differentiation of postnatal cerebellar glial progenitors is controlled by Bmi1 through BMP pathway inhibition. Glia. 2011;59:1118-31 pubmed publisher
    ..Thus modulation of Bmi1 expression in glial progenitors may represent a key event in determining the differentiation potential of these cells. ..
  37. Huber P, Birdsey G, Lumb M, Prowse D, Perkins T, Knight D, et al. Peroxisomal import of human alanine:glyoxylate aminotransferase requires ancillary targeting information remote from its C terminus. J Biol Chem. 2005;280:27111-20 pubmed
    ..This interaction might reshape the tetratricopeptide repeat domain allosterically, enabling it to accept KKL as a functional PTS1. ..
  38. McArthur S, Robinson I, Gillies G. Novel ontogenetic patterns of sexual differentiation in arcuate nucleus GHRH neurons revealed in GHRH-enhanced green fluorescent protein transgenic mice. Endocrinology. 2011;152:607-17 pubmed publisher
    ..This information adds to our knowledge of processes that underpin the emergence of sex-specific GH secretory dynamics and hence biological activity of this pleiotropic hormone. ..
  39. Lee J, Hargest R, Wasan H, Phillips R. Liposome-mediated adenomatous polyposis coli gene therapy: a novel anti-adenoma strategy in multiple intestinal neoplasia mouse model. Dis Colon Rectum. 2004;47:2105-13 pubmed
    ..This in vivo study provides evidence to support a novel anti-adenoma strategy using enteral adenomatous polyposis coli gene replacement therapy. ..
  40. Abramov A, Fraley C, Diao C, Winkfein R, Colicos M, Duchen M, et al. Targeted polyphosphatase expression alters mitochondrial metabolism and inhibits calcium-dependent cell death. Proc Natl Acad Sci U S A. 2007;104:18091-6 pubmed
    ..These results demonstrate a crucial role played by polyP in mitochondrial function of mammalian cells. ..
  41. McBride D, Buckle A, van Heyningen V, Kleinjan D. DNaseI hypersensitivity and ultraconservation reveal novel, interdependent long-range enhancers at the complex Pax6 cis-regulatory region. PLoS ONE. 2011;6:e28616 pubmed publisher
  42. Jones M, Craik J, Gibbins J, Poole A. Regulation of SHP-1 tyrosine phosphatase in human platelets by serine phosphorylation at its C terminus. J Biol Chem. 2004;279:40475-83 pubmed
  43. Greaves J, Chamberlain L. Dual role of the cysteine-string domain in membrane binding and palmitoylation-dependent sorting of the molecular chaperone cysteine-string protein. Mol Biol Cell. 2006;17:4748-59 pubmed
    ..These results reveal a dual function of the cysteine-string domain: initial membrane binding and palmitoylation-dependent sorting. ..
  44. Lewis M, Nichols B, Prescianotto Baschong C, Riezman H, Pelham H. Specific retrieval of the exocytic SNARE Snc1p from early yeast endosomes. Mol Biol Cell. 2000;11:23-38 pubmed
    ..We propose that these represent early endosomes in which sorting of Snc1p and late Golgi proteins occurs, and that transport can occur directly from them to the Golgi apparatus. ..
  45. Delon C, Manifava M, Wood E, Thompson D, Krugmann S, Pyne S, et al. Sphingosine kinase 1 is an intracellular effector of phosphatidic acid. J Biol Chem. 2004;279:44763-74 pubmed
    ..The SK1-PA interaction maps to the C terminus of SK1 and is independent of catalytic activity or of the diacylglycerol kinase-like domain of the enzyme. Thus SK1 constitutes a novel, physiologically relevant PA effector. ..
  46. Vallier L, Alexander M, Pedersen R. Conditional gene expression in human embryonic stem cells. Stem Cells. 2007;25:1490-7 pubmed
    ..Disclosure of potential conflicts of interest is found at the end of this article. ..
  47. Kershaw M, Talbot N. Genome-wide functional analysis reveals that infection-associated fungal autophagy is necessary for rice blast disease. Proc Natl Acad Sci U S A. 2009;106:15967-72 pubmed publisher
    ..A genome-wide analysis therefore demonstrates the importance of infection-associated, nonselective autophagy for the establishment of rice blast disease. ..
  48. Calcagno Pizarelli A, Negrete Urtasun S, Denison S, Rudnicka J, Bussink H, MĂșnera Huertas T, et al. Establishment of the ambient pH signaling complex in Aspergillus nidulans: PalI assists plasma membrane localization of PalH. Eukaryot Cell. 2007;6:2365-75 pubmed
  49. Friocourt G, Kanatani S, Tabata H, Yozu M, Takahashi T, Antypa M, et al. Cell-autonomous roles of ARX in cell proliferation and neuronal migration during corticogenesis. J Neurosci. 2008;28:5794-805 pubmed publisher
    ..However, our data suggest that ARX is not directly involved in GABAergic cell fate specification. Overall, these results identify multiple and distinct cell-autonomous roles for ARX in corticogenesis. ..
  50. Becker H, Sturm A, Bron J, Schirmer K, Bury N. The A/B domain of the teleost glucocorticoid receptors influences partial nuclear localization in the absence of hormone. Endocrinology. 2008;149:4567-76 pubmed publisher
    ..This is unrelated to the difference in the trout GRs transactivation sensitivity, which is determined by the receptor's E-domains. ..
  51. Alcock J, Sottile V. Dynamic distribution and stem cell characteristics of Sox1-expressing cells in the cerebellar cortex. Cell Res. 2009;19:1324-33 pubmed publisher
  52. Donier E, Rugiero F, Okuse K, Wood J. Annexin II light chain p11 promotes functional expression of acid-sensing ion channel ASIC1a. J Biol Chem. 2005;280:38666-72 pubmed
  53. Shen S, Lang B, Nakamoto C, Zhang F, Pu J, Kuan S, et al. Schizophrenia-related neural and behavioral phenotypes in transgenic mice expressing truncated Disc1. J Neurosci. 2008;28:10893-904 pubmed publisher
    ..These abnormalities in Disc1(tr) transgenic mice are consistent with findings in severe schizophrenia. ..
  54. Nesbit M, Bowl M, Harding B, Ali A, Ayala A, Crowe C, et al. Characterization of GATA3 mutations in the hypoparathyroidism, deafness, and renal dysplasia (HDR) syndrome. J Biol Chem. 2004;279:22624-34 pubmed
  55. Parra Palau J, Scheper G, Wilson M, Proud C. Features in the N and C termini of the MAPK-interacting kinase Mnk1 mediate its nucleocytoplasmic shuttling. J Biol Chem. 2003;278:44197-204 pubmed
    ..Mutations that reduce the binding of Mnk1 to eIF4G in vivo and in vitro also decrease the ability of Mnk1 to enhance eIF4E phosphorylation in vivo, underlining the importance of the eIF4G-Mnk1 interaction in this process. ..
  56. Mant A, Elliott D, Eyers P, O Kelly I. Protein kinase A is central for forward transport of two-pore domain potassium channels K2P3.1 and K2P9.1. J Biol Chem. 2011;286:14110-9 pubmed publisher
    ..All of our findings support the conclusion that cAMP-dependent protein kinase is responsible for the phosphorylation of the terminal serine in both K2P3.1 and K2P9.1. ..
  57. Pozo K, Cingolani L, Bassani S, Laurent F, Passafaro M, Goda Y. ?3 integrin interacts directly with GluA2 AMPA receptor subunit and regulates AMPA receptor expression in hippocampal neurons. Proc Natl Acad Sci U S A. 2012;109:1323-8 pubmed publisher
    ..Our findings identify an integrin family member, ?3, as a direct interactor of an AMPAR subunit and provide molecular insights into how this cell-adhesion protein regulates the composition of cell-surface AMPARs. ..
  58. Weylandt K, Valverde M, Nobles M, Raguz S, Amey J, Diaz M, et al. Human ClC-3 is not the swelling-activated chloride channel involved in cell volume regulation. J Biol Chem. 2001;276:17461-7 pubmed
    ..This distribution is also inconsistent with the previous proposed role in cell volume regulation. ..
  59. Markiewicz E, Dechat T, Foisner R, Quinlan R, Hutchison C. Lamin A/C binding protein LAP2alpha is required for nuclear anchorage of retinoblastoma protein. Mol Biol Cell. 2002;13:4401-13 pubmed
    ..Together, these data suggest that hypophosphorylated Rb is anchored in the nucleus by the interaction of pocket C with LAP2alpha-lamin A/C complexes. ..
  60. Ganesh K, Adam S, Taylor B, Simpson P, Rada C, Neuberger M. CTNNBL1 is a novel nuclear localization sequence-binding protein that recognizes RNA-splicing factors CDC5L and Prp31. J Biol Chem. 2011;286:17091-102 pubmed publisher
    ..Thus, CTNNBL1 is a novel NLS-binding protein, distinct from karyopherin ?s, with the results suggesting a possible role in the selective intranuclear targeting or interactions of some splicing-associated complexes. ..
  61. Bassi Z, Audusseau M, Riparbelli M, Callaini G, D Avino P. Citron kinase controls a molecular network required for midbody formation in cytokinesis. Proc Natl Acad Sci U S A. 2013;110:9782-7 pubmed publisher
    ..Our findings indicate that CIT-K is likely to act at the top of the midbody-formation hierarchy by connecting and regulating a molecular network of contractile ring components and microtubule-associated proteins...
  62. Walsh E, Baron M, Rennie L, Monaghan P, Anderson J, Barrett T. Recombinant rinderpest vaccines expressing membrane-anchored proteins as genetic markers: evidence of exclusion of marker protein from the virus envelope. J Virol. 2000;74:10165-75 pubmed
    ..Both viruses were effective in stimulating protective immunity against RPV and antibody responses to the marker protein in all animals when tested in a cattle vaccination trial...
  63. Young M, Fisher J, Fountain S, Ford R, North R, Khakh B. Molecular shape, architecture, and size of P2X4 receptors determined using fluorescence resonance energy transfer and electron microscopy. J Biol Chem. 2008;283:26241-51 pubmed publisher
    ..1 nm, which closely matches FRET data. Thus, our data provide the first views of the architecture, shape, and size of single P2X receptors, furthering our understanding of this important family of ligand-gated ion channels. ..
  64. Edward R. Red/far-red fluorescing DNA-specific anthraquinones for nucl:cyto segmentation and viability reporting in cell-based assays. Methods Enzymol. 2012;505:23-45 pubmed publisher
    ..This far-red viability dye can be more widely combined with other fluorescent reporters to reveal temporally separated events and shows negligible cytotoxicity as determined by sensitive bioassays. ..
  65. Dunand Sauthier I, Walker C, Wilkinson C, Gordon C, Crane R, Norbury C, et al. Sum1, a component of the fission yeast eIF3 translation initiation complex, is rapidly relocalized during environmental stress and interacts with components of the 26S proteasome. Mol Biol Cell. 2002;13:1626-40 pubmed
    ..Furthermore, Sum1 interacts with the Mts2 and Mts4 components of the 26S proteasome. These data indicate a functional link between components of the structurally related eIF3 translation initiation and 26S proteasome complexes. ..
  66. Hughes D, Mackie M, Nagy G, Riddell J, Maxwell D, Szabo G, et al. P boutons in lamina IX of the rodent spinal cord express high levels of glutamic acid decarboxylase-65 and originate from cells in deep medial dorsal horn. Proc Natl Acad Sci U S A. 2005;102:9038-43 pubmed
    ..They also provide a means of identifying P boutons that will be important in studies of the organization of presynaptic control of Ia afferents. ..
  67. Caunt C, Finch A, Sedgley K, Oakley L, Luttrell L, McArdle C. Arrestin-mediated ERK activation by gonadotropin-releasing hormone receptors: receptor-specific activation mechanisms and compartmentalization. J Biol Chem. 2006;281:2701-10 pubmed
  68. Waters L, Strong S, Ferlemann E, Oka O, Muskett F, Veverka V, et al. Structure of the tandem MA-3 region of Pdcd4 protein and characterization of its interactions with eIF4A and eIF4G: molecular mechanisms of a tumor suppressor. J Biol Chem. 2011;286:17270-80 pubmed publisher
    ..Pdcd4 has also been reported to interact directly with the middle region of eIF4G, however, we were unable to obtain any evidence for even a weak, transient direct interaction. ..
  69. Akhtar N, Hotchin N. RAC1 regulates adherens junctions through endocytosis of E-cadherin. Mol Biol Cell. 2001;12:847-62 pubmed
    ..Our data suggest that Rac1 regulates adherens junctions via clathrin independent endocytosis of E-cadherin...
  70. Tomas A, Moss S. Calcium- and cell cycle-dependent association of annexin 11 with the nuclear envelope. J Biol Chem. 2003;278:20210-6 pubmed
  71. Capalbo L, D Avino P, Archambault V, Glover D. Rab5 GTPase controls chromosome alignment through Lamin disassembly and relocation of the NuMA-like protein Mud to the poles during mitosis. Proc Natl Acad Sci U S A. 2011;108:17343-8 pubmed publisher
    ..Our results indicate a role for Rab5 in mitosis and reinforce the emerging view of the contributions made by cell membrane dynamics to spindle function. ..
  72. Reinicke A, Hutchinson J, Magee A, Mastroeni P, Trowsdale J, Kelly A. A Salmonella typhimurium effector protein SifA is modified by host cell prenylation and S-acylation machinery. J Biol Chem. 2005;280:14620-7 pubmed
    ..This is the only known example of a bacterial effector protein that is modified both by mammalian cell S-acylation and prenylation machinery. ..
  73. Di Cola A, Bailey S, Robinson C. The thylakoid delta pH/delta psi are not required for the initial stages of Tat-dependent protein transport in tobacco protoplasts. J Biol Chem. 2005;280:41165-70 pubmed
    ..We conclude that unknown factors play an influential role in both the mechanism and energetics of this system under in vivo conditions. ..
  74. Kilaru S, Collins C, Hartley A, Bailey A, Foster G. Establishing molecular tools for genetic manipulation of the pleuromutilin-producing fungus Clitopilus passeckerianus. Appl Environ Microbiol. 2009;75:7196-204 pubmed publisher
    ..passeckerianus. The molecular tools developed in this study should facilitate studies aimed at gene isolation or characterization in this pharmaceutically important species. ..
  75. Crisp S, Evers J, Bate M. Endogenous patterns of activity are required for the maturation of a motor network. J Neurosci. 2011;31:10445-50 pubmed publisher
    ..We conclude that there is a period during which endogenous patterns of neuronal activity are required for the normal development of motor circuits in Drosophila. ..
  76. Geerling J, Kim M, Mahoney C, Abbott S, Agostinelli L, Garfield A, et al. Genetic identity of thermosensory relay neurons in the lateral parabrachial nucleus. Am J Physiol Regul Integr Comp Physiol. 2016;310:R41-54 pubmed publisher
    ..These findings improve our understanding of LPB organization and reveal that Pdyn-IRES-Cre mice provide genetic access to warm-activated, FoxP2+ glutamatergic neurons in PBdL, many of which project to the hypothalamus. ..
  77. Rylott E, Rogers C, Gilday A, Edgell T, Larson T, Graham I. Arabidopsis mutants in short- and medium-chain acyl-CoA oxidase activities accumulate acyl-CoAs and reveal that fatty acid beta-oxidation is essential for embryo development. J Biol Chem. 2003;278:21370-7 pubmed
    ..This is the first demonstration of the effects of eliminating (short-chain) beta-oxidation capacity in plants and shows that a functional beta-oxidation cycle is essential in the early stages of embryo development. ..
  78. Hong Y, Stanley J, van Wezel R. Novel system for the simultaneous analysis of geminivirus DNA replication and plant interactions in Nicotiana benthamiana. J Virol. 2003;77:13315-22 pubmed
    ..This defect correlates with possible loss of Rep nuclear localization and inability to trigger the host defense mechanism resembling a hypersensitive response. ..
  79. Guti rrez Marcos J, Dal Pr M, Giulini A, Costa L, Gavazzi G, Cordelier S, et al. empty pericarp4 encodes a mitochondrion-targeted pentatricopeptide repeat protein necessary for seed development and plant growth in maize. Plant Cell. 2007;19:196-210 pubmed publisher
    ..Molecular analyses further suggest that EMP4 is necessary to regulate the correct expression of a small subset of mitochondrial transcripts in the endosperm...
  80. Arpino J, Czapinska H, Piasecka A, Edwards W, Barker P, Gajda M, et al. Structural basis for efficient chromophore communication and energy transfer in a constructed didomain protein scaffold. J Am Chem Soc. 2012;134:13632-40 pubmed publisher
  81. Zhao S, Maxwell S, Jimenez Beristain A, Vives J, Kuehner E, Zhao J, et al. Generation of embryonic stem cells and transgenic mice expressing green fluorescence protein in midbrain dopaminergic neurons. Eur J Neurosci. 2004;19:1133-40 pubmed
    ..These mice would also provide a useful tool to study DA fate determination from embryo- or adult-derived neural stem cells. ..
  82. Varadi A, Tsuboi T, Rutter G. Myosin Va transports dense core secretory vesicles in pancreatic MIN6 beta-cells. Mol Biol Cell. 2005;16:2670-80 pubmed
    ..We conclude that MyoVa-driven movements of vesicles along the cortical actin network are essential for the terminal stages of regulated exocytosis in beta-cells. ..
  83. Cloix C, Kaiserli E, Heilmann M, Baxter K, Brown B, O Hara A, et al. C-terminal region of the UV-B photoreceptor UVR8 initiates signaling through interaction with the COP1 protein. Proc Natl Acad Sci U S A. 2012;109:16366-70 pubmed publisher
    ..Furthermore, we show that C27 interacts in yeast with the REPRESSOR OF UV-B PHOTOMORPHOGENESIS proteins, RUP1 and RUP2, which are negative regulators of UVR8 function. Hence the C27 region has a key role in UVR8 function. ..
  84. Escobar N, Haupt S, Thow G, Boevink P, Chapman S, Oparka K. High-throughput viral expression of cDNA-green fluorescent protein fusions reveals novel subcellular addresses and identifies unique proteins that interact with plasmodesmata. Plant Cell. 2003;15:1507-23 pubmed
    ..This virus-based system represents a method for high-throughput functional genomic study of plant cell organelles and allows the identification of unique proteins that associate with specific subcompartments within organelles...
  85. Ryabov E, van Wezel R, Walsh J, Hong Y. Cell-to-Cell, but not long-distance, spread of RNA silencing that is induced in individual epidermal cells. J Virol. 2004;78:3149-54 pubmed
    ..TCV coinfection arrested TCV-GFPDeltaCP-mediated local induction of RNA silencing. Possible mechanisms involved in cell-to-cell and long-distance spread of RNA silencing are discussed...
  86. Ray N, Nenninger A, Mullineaux C, Robinson C. Location and mobility of twin arginine translocase subunits in the Escherichia coli plasma membrane. J Biol Chem. 2005;280:17961-8 pubmed
    ..The significance of the punctate TatA/B/C-GFP is unclear; fluorescence recovery after photobleaching measurements show that these pools of proteins are immobile, whereas nonaggregated proteins are highly mobile in the plasma membrane. ..
  87. Harris H, Farquhar M, Mee C, Davis C, Reynolds G, Jennings A, et al. CD81 and claudin 1 coreceptor association: role in hepatitis C virus entry. J Virol. 2008;82:5007-20 pubmed publisher
  88. Fatimathas L, Moss S. Characterisation of the sarcoidosis-associated variant of annexin A11. Gen Physiol Biophys. 2009;28 Spec No Focus:F29-38 pubmed
    ..This does not rule out functional affects in the extracellular milieu, in cytokinesis, nuclear envelope breakdown or in response to other intracellular signals. ..
  89. Ross K, Yang L, Dower S, Volpe F, Guesdon F. Identification of threonine 66 as a functionally critical residue of the interleukin-1 receptor-associated kinase. J Biol Chem. 2002;277:37414-21 pubmed
    ..These properties in turn determine key aspects of the signaling function of IRAK. ..
  90. Guo K, Lukacik P, Papagrigoriou E, Meier M, Lee W, Adamski J, et al. Characterization of human DHRS6, an orphan short chain dehydrogenase/reductase enzyme: a novel, cytosolic type 2 R-beta-hydroxybutyrate dehydrogenase. J Biol Chem. 2006;281:10291-7 pubmed
    ..The properties determined for DHRS6 suggest a possible physiological role in cytosolic ketone body utilization, either as a secondary system for energy supply in starvation or to generate precursors for lipid and sterol synthesis. ..
  91. Banks G, Haas M, Line S, Shepherd H, AlQatari M, Stewart S, et al. Behavioral and other phenotypes in a cytoplasmic Dynein light intermediate chain 1 mutant mouse. J Neurosci. 2011;31:5483-94 pubmed publisher
    ..These results demonstrate the important role that dynein-controlled processes play in the correct development and function of the mammalian nervous system. ..
  92. Gibson V, Benson R, Bryson K, McInnes I, Rush C, Grassia G, et al. A novel method to allow noninvasive, longitudinal imaging of the murine immune system in vivo. Blood. 2012;119:2545-51 pubmed publisher
    ..Thus, we provide the first demonstration of a method for repeated, noninvasive, in vivo imaging of lymphocyte behavior. ..