Experts and Doctors on genetic vectors in South Africa
Locale: South Africa
Topic: genetic vectors
du Plessis M, Cloete M, Aitchison H, van Dijk A. Protein aggregation complicates the development of baculovirus-expressed African horsesickness virus serotype 5 VP2 subunit vaccines. Onderstepoort J Vet Res. 1998;65:321-9 pubmed
..Further investigation is needed to limit formation of insoluble aggregates and optimize conditions for producing VP2 in a form capable of inducing protective immunity. ..
Weinberg M, Ely A, Passman M, Mufamadi S, Arbuthnot P. Effective anti-hepatitis B virus hammerhead ribozymes derived from multimeric precursors. Oligonucleotides. 2007;17:104-12 pubmed
..The approach described here is potentially useful for several applications, such as generation of RNA interference (RNAi) effectors, which require rapid and inexpensive generation of defined RNA sequences. ..
Maree S, Paweska J. Preparation of recombinant African horse sickness virus VP7 antigen via a simple method and validation of a VP7-based indirect ELISA for the detection of group-specific IgG antibodies in horse sera. J Virol Methods. 2005;125:55-65 pubmed
..The TG-ROC analysis was used for the selection of the cut-off value. At a cut-off of 11.9 of the high positive control serum (percentage positivity), the I-ELISA specificity was 100%, sensitivity 99.4%, and the Jouden index was 0.99...
Wallace D, Weyer J, Nel L, Viljoen G. Improved method for the generation and selection of homogeneous lumpy skin disease virus (SA-Neethling) recombinants. J Virol Methods. 2007;146:52-60 pubmed
Meyers A, Chakauya E, Shephard E, Tanzer F, Maclean J, Lynch A, et al
. Expression of HIV-1 antigens in plants as potential subunit vaccines. BMC Biotechnol. 2008;8:53 pubmed publisher
..The p17/p24 protein effectively boosted T cell and humoral responses in mice primed by the DNA vaccine pTHGagC, showing that this plant-produced protein has potential for use as a vaccine. ..
Regnard G, Halley Stott R, Tanzer F, Hitzeroth I, Rybicki E. High level protein expression in plants through the use of a novel autonomously replicating geminivirus shuttle vector. Plant Biotechnol J. 2010;8:38-46 pubmed publisher
..We anticipate that this new high-yielding plant expression vector will contribute towards the development of a viable plant production platform for vaccine candidates and other pharmaceuticals. ..
Chubb A, Woodman Z, da Silva Tatley F, Hoffmann H, Scholle R, Ehlers M. Identification of Mycobacterium tuberculosis signal sequences that direct the export of a leaderless beta-lactamase gene product in Escherichia coli. Microbiology. 1998;144 ( Pt 6):1619-29 pubmed
..It is concluded that expression of beta-lactamase gene fusions in E. coli provides a useful system for the identification and analysis of M. tuberculosis signal-sequence-encoding genes. ..
Pretorius A, Van Kleef M, Collins N, Tshikudo N, Louw E, Faber F, et al
. A heterologous prime/boost immunisation strategy protects against virulent E. ruminantium Welgevonden needle challenge but not against tick challenge. Vaccine. 2008;26:4363-71 pubmed publisher
..Thus this heterologous prime/boost immunisation strategy had not conferred any significant protection against a field challenge...
van Zyl L, van Munster J, Rawlings D. Construction of arsB and tetH mutants of the sulfur-oxidizing bacterium Acidithiobacillus caldus by marker exchange. Appl Environ Microbiol. 2008;74:5686-94 pubmed publisher
..caldus arsB and tetH mutants, and the effects of these mutations on cell growth in the presence of arsenic and by means of tetrathionate oxidation were demonstrated...