Experts and Doctors on genetic complementation test in Belgium


Locale: Belgium
Topic: genetic complementation test

Top Publications

  1. Maes T, Van de Steene N, Zethof J, Karimi M, D hauw M, Mares G, et al. Petunia Ap2-like genes and their role in flower and seed development. Plant Cell. 2001;13:229-44 pubmed
    ..Although the interspecific complementation proves that PhAp2A encodes a genuine Ap2 ortholog from petunia, additional factors may be involved in the control of perianth identity in this species. ..
  2. Kellner H, Luis P, Portetelle D, Vandenbol M. Screening of a soil metatranscriptomic library by functional complementation of Saccharomyces cerevisiae mutants. Microbiol Res. 2011;166:360-8 pubmed publisher
    ..The sequenced insert showed strong similarity to a basidiomycete acid phosphatase (60% identity to Postia placenta). ..
  3. Vergauwen B, Pauwels F, Vaneechoutte M, Van Beeumen J. Exogenous glutathione completes the defense against oxidative stress in Haemophilus influenzae. J Bacteriol. 2003;185:1572-81 pubmed
    ..coli. Finally, we demonstrated that cysteine is an essential amino acid for growth and that cystine, GSH, glutathione amide, and cysteinylglycine can be catabolized in order to complement cysteine deficiency. ..
  4. Pauwels F, Vergauwen B, Van Beeumen J. Physiological characterization of Haemophilus influenzae Rd deficient in its glutathione-dependent peroxidase PGdx. J Biol Chem. 2004;279:12163-70 pubmed
    ..This observed feature was alleviated by complementation with the functional gene or with the addition of catalase. ..
  5. de Chial M, Ghysels B, Beatson S, Geoffroy V, Meyer J, Pattery T, et al. Identification of type II and type III pyoverdine receptors from Pseudomonas aeruginosa. Microbiology. 2003;149:821-31 pubmed
    ..All three receptors posses an N-terminal extension of about 70 amino acids, similar to FecA of Escherichia coli, but only FpvAI has a TAT export sequence at its N-terminal end. ..
  6. Vanholme B, Kast P, Haegeman A, Jacob J, Grunewald W, Gheysen G. Structural and functional investigation of a secreted chorismate mutase from the plant-parasitic nematode Heterodera schachtii in the context of related enzymes from diverse origins. Mol Plant Pathol. 2009;10:189-200 pubmed publisher
    ..Finally, we show how the modular design of the protein is mirrored in the genomic sequence by the intron/exon organization, suggesting exon shuffling as a mechanism for the evolutionary assembly of this protein. ..
  7. Leimanis S, Hoyez N, Hubert S, Laschet M, Sauvage E, Brasseur R, et al. PBP5 complementation of a PBP3 deficiency in Enterococcus hirae. J Bacteriol. 2006;188:6298-307 pubmed
    ..The P197-N209 segment (site E) in one of these domains seemed to be particularly important, as single and double mutations reduced or almost completely abolished, respectively, the action of PBP5. ..
  8. Aendekerk S, Ghysels B, Cornelis P, Baysse C. Characterization of a new efflux pump, MexGHI-OpmD, from Pseudomonas aeruginosa that confers resistance to vanadium. Microbiology. 2002;148:2371-81 pubmed
    ..In conclusion, it is believed that the MexGHI-OpmD pump is probably involved in AHL homeostasis in P. aeruginosa. ..
  9. Baert B, Baysse C, Matthijs S, Cornelis P. Multiple phenotypic alterations caused by a c-type cytochrome maturation ccmC gene mutation in Pseudomonas aeruginosa. Microbiology. 2008;154:127-38 pubmed publisher
    ..In conclusion, we have demonstrated that CcmC is a key determinant for cytochrome c biogenesis, pyoverdine maturation, and expression of some quorum sensing-regulated traits...

More Information


  1. Thevissen K, Cammue B, Lemaire K, Winderickx J, Dickson R, Lester R, et al. A gene encoding a sphingolipid biosynthesis enzyme determines the sensitivity of Saccharomyces cerevisiae to an antifungal plant defensin from dahlia (Dahlia merckii). Proc Natl Acad Sci U S A. 2000;97:9531-6 pubmed
    ..Our data support a model in which membrane patches containing sphingolipids act as binding sites for DmAMP1 or, alternatively, are required to anchor membrane or cell wall-associated proteins, which themselves interact with DmAMP1. ..
  2. Vinckx T, Wei Q, Matthijs S, Cornelis P. The Pseudomonas aeruginosa oxidative stress regulator OxyR influences production of pyocyanin and rhamnolipids: protective role of pyocyanin. Microbiology. 2010;156:678-86 pubmed publisher
  3. Van Damme D, De Rybel B, Gudesblat G, Demidov D, Grunewald W, De Smet I, et al. Arabidopsis ? Aurora kinases function in formative cell division plane orientation. Plant Cell. 2011;23:4013-24 pubmed publisher
    ..Taken together, our results put forward a central role for ? Aurora kinases in regulating formative division plane orientation throughout development. ..
  4. Dozot M, Boigegrain R, Delrue R, Hallez R, Ouahrani Bettache S, Danese I, et al. The stringent response mediator Rsh is required for Brucella melitensis and Brucella suis virulence, and for expression of the type IV secretion system virB. Cell Microbiol. 2006;8:1791-802 pubmed
  5. Thevissen K, Idkowiak Baldys J, Im Y, Takemoto J, Francois I, Ferket K, et al. SKN1, a novel plant defensin-sensitivity gene in Saccharomyces cerevisiae, is implicated in sphingolipid biosynthesis. FEBS Lett. 2005;579:1973-7 pubmed
    ..These results show that SKN1, together with IPT1, is involved in sphingolipid biosynthesis in S. cerevisiae. ..
  6. Lammertyn E, Van Mellaert L, Meyen E, Lebeau I, De Buck E, Anne J, et al. Molecular and functional characterization of type I signal peptidase from Legionella pneumophila. Microbiology. 2004;150:1475-83 pubmed
    ..Finally it was shown by in vivo experiments that L. pneumophila LepB is a target for (5S,6S)-6-[(R)-acetoxyethyl]-penem-3-carboxylate, a specific inhibitor of type I SPases. ..
  7. Van Leeuwen T, Demaeght P, Osborne E, Dermauw W, Gohlke S, Nauen R, et al. Population bulk segregant mapping uncovers resistance mutations and the mode of action of a chitin synthesis inhibitor in arthropods. Proc Natl Acad Sci U S A. 2012;109:4407-12 pubmed publisher
    ..More generally, our genetic mapping approach should be powerful for high-resolution mapping of simple traits (resistance or otherwise) in arthropods. ..
  8. Paindavoine P, Rolin S, Van Assel S, Geuskens M, Jauniaux J, Dinsart C, et al. A gene from the variant surface glycoprotein expression site encodes one of several transmembrane adenylate cyclases located on the flagellum of Trypanosoma brucei. Mol Cell Biol. 1992;12:1218-25 pubmed
    ..brucei (S. Rolin, S. Halleux, J. Van Sande, J. E. Dumont, E. Pays, and M. Steinert. Exp. Parasitol. 71:350-352, 1990). Our data suggest that the trypanosome cyclases are not properly regulated in yeast cells...
  9. Zimon M, Baets J, Almeida Souza L, De Vriendt E, Nikodinovic J, Parman Y, et al. Loss-of-function mutations in HINT1 cause axonal neuropathy with neuromyotonia. Nat Genet. 2012;44:1080-3 pubmed publisher
    ..Our study provides evidence that loss of functional HINT1 protein results in a distinct phenotype of autosomal recessive axonal neuropathy with neuromyotonia...
  10. Huysman M, Fortunato A, Matthijs M, Costa B, Vanderhaeghen R, Van Den Daele H, et al. AUREOCHROME1a-mediated induction of the diatom-specific cyclin dsCYC2 controls the onset of cell division in diatoms (Phaeodactylum tricornutum). Plant Cell. 2013;25:215-28 pubmed publisher
  11. Dedeurwaerder A, Olyslaegers D, Desmarets L, Roukaerts I, Theuns S, Nauwynck H. ORF7-encoded accessory protein 7a of feline infectious peritonitis virus as a counteragent against IFN-?-induced antiviral response. J Gen Virol. 2014;95:393-402 pubmed publisher
    ..These results indicate that FIPV 7a protein is a type I IFN antagonist and protects the virus from the antiviral state induced by IFN, but it needs the presence of ORF3-encoded proteins to exert its antagonistic function. ..
  12. Bizimungu C, De Neve N, Burny A, Bach S, Bontemps F, Portetelle D, et al. Expression in a RabGAP yeast mutant of two human homologues, one of which is an oncogene. Biochem Biophys Res Commun. 2003;310:498-504 pubmed
    ..As two highly conserved amino acids, including the catalytic arginine, are mutated in the oncTre210p TBC domain, we restored these two amino acids and expressed the modified Tre2 cDNA in the yeast mutant. ..
  13. Eyckerman S, Verhee A, der Heyden J, Lemmens I, Ostade X, Vandekerckhove J, et al. Design and application of a cytokine-receptor-based interaction trap. Nat Cell Biol. 2001;3:1114-9 pubmed
    ..Importantly, this approach places protein-protein interactions in their normal physiological context, and is especially applicable to the in situ analysis of signal transduction pathways. ..
  14. Van Huffel C, Dubois E, Messenguy F. Cloning and sequencing of Schizosaccharomyces pombe car1 gene encoding arginase. Expression of the arginine anabolic and catabolic genes in response to arginine and related metabolites. Yeast. 1994;10:923-33 pubmed
    ..cerevisiae, and also indicates that in S. pombe enzyme compartmentation is not always sufficient to control the arginine metabolic flux. ..
  15. Marini A, Matassi G, Raynal V, Andre B, Cartron J, Cherif Zahar B. The human Rhesus-associated RhAG protein and a kidney homologue promote ammonium transport in yeast. Nat Genet. 2000;26:341-4 pubmed
    ..Our results provide the first experimental evidence for a direct role of RhAG and RhGK in ammonium transport. These findings are of high interest, because no specific ammonium transport system has been characterized so far in human. ..
  16. Michiels K, Vanderleyden J, Van Gool A, Signer E. Isolation and characterization of Azospirillum brasilense loci that correct Rhizobium meliloti exoB and exoC mutations. J Bacteriol. 1988;170:5401-4 pubmed
    ..It was found that these mutants failed to produce the wild-type high-molecular-weight EPS, but instead produced EPSs of lower molecular weight...
  17. Van Lijsebettens M, Vanderhaeghen R, De Block M, Bauw G, Villarroel R, Van Montagu M. An S18 ribosomal protein gene copy at the Arabidopsis PFL locus affects plant development by its specific expression in meristems. EMBO J. 1994;13:3378-88 pubmed
    ..In analogy with this, an additional, developmentally regulated gene copy might be expected for all ribosomal proteins. ..
  18. Andre B, Hein C, Grenson M, Jauniaux J. Cloning and expression of the UGA4 gene coding for the inducible GABA-specific transport protein of Saccharomyces cerevisiae. Mol Gen Genet. 1993;237:17-25 pubmed
    ..In the absence of the pleiotropic UGA43/DAL80 repressor, UGA4 is constitutively expressed at high level. ..
  19. Iraqui I, Vissers S, Cartiaux M, Urrestarazu A. Characterisation of Saccharomyces cerevisiae ARO8 and ARO9 genes encoding aromatic aminotransferases I and II reveals a new aminotransferase subfamily. Mol Gen Genet. 1998;257:238-48 pubmed
    ..An autonomously replicating sequence (ARS) element is located between the ARO8 gene and YGL201c which encodes a protein of the minichromosome maintenance family. ..
  20. Crabeel M, Soetens O, De Rijcke M, Pratiwi R, Pankiewicz R. The ARG11 gene of Saccharomyces cerevisiae encodes a mitochondrial integral membrane protein required for arginine biosynthesis. J Biol Chem. 1996;271:25011-8 pubmed
    ..Arg11p thus appears to fulfill a partially redundant function requiring its 27 carboxyl-terminal amino acids. As a working hypothesis, we propose that Arg11p participates in the export of matrix-made ornithine into the cytosol. ..
  21. Hofte M, Dong Q, Kourambas S, Krishnapillai V, Sherratt D, Mergeay M. The sss gene product, which affects pyoverdin production in Pseudomonas aeruginosa 7NSK2, is a site-specific recombinase. Mol Microbiol. 1994;14:1011-20 pubmed
    ..The sss gene of P. aeruginosa could restore site-specific recombination at cer in an E. coli xerC mutant and the E. coli xerC gene could complement a genomic sss mutation in P. aeruginosa. ..
  22. Lebeer S, Verhoeven T, Francius G, Schoofs G, Lambrichts I, Dufr ne Y, et al. Identification of a Gene Cluster for the Biosynthesis of a Long, Galactose-Rich Exopolysaccharide in Lactobacillus rhamnosus GG and Functional Analysis of the Priming Glycosyltransferase. Appl Environ Microbiol. 2009;75:3554-63 pubmed publisher
    ..rhamnosus GG, possibly because of less shielding of adhesins such as fimbria-like structures...
  23. Meulemans A, Seneca S, Pribyl T, Smet J, Alderweirldt V, Waeytens A, et al. Defining the pathogenesis of the human Atp12p W94R mutation using a Saccharomyces cerevisiae yeast model. J Biol Chem. 2010;285:4099-109 pubmed publisher
    ..Instead, we provide evidence that the primary effect of the substitution is on the dynamic properties of Atp12p. ..
  24. Rotthier A, Auer Grumbach M, Janssens K, Baets J, Penno A, Almeida Souza L, et al. Mutations in the SPTLC2 subunit of serine palmitoyltransferase cause hereditary sensory and autonomic neuropathy type I. Am J Hum Genet. 2010;87:513-22 pubmed publisher
    ..We further show that HSAN-I is consistently associated with an increased formation of the neurotoxic 1-deoxysphinganine, suggesting a common pathomechanism for HSAN-I...
  25. Ghys K, Fransen M, Mannaerts G, Van Veldhoven P. Functional studies on human Pex7p: subcellular localization and interaction with proteins containing a peroxisome-targeting signal type 2 and other peroxins. Biochem J. 2002;365:41-50 pubmed
    ..The GFP fusion proteins, tagged at either the N- or C-terminus, were able to restore PTS2 import in rhizomelic chondrodysplasia punctata fibroblasts, and Pex7p-GFP was located both in the lumen of peroxisomes and in the cytosol...
  26. Heyman J, Van Den Daele H, De Wit K, Boudolf V, Berckmans B, Verkest A, et al. Arabidopsis ULTRAVIOLET-B-INSENSITIVE4 maintains cell division activity by temporal inhibition of the anaphase-promoting complex/cyclosome. Plant Cell. 2011;23:4394-410 pubmed publisher
    ..We conclude that UVI4 regulates the temporal inactivation of APC/C during DNA replication, allowing CYCA2;3 to accumulate above the level required for entering mitosis, and thereby regulates the meristem size and plant growth rate. ..
  27. Qiu H, Dubois E, Broen P, Messenguy F. Functional analysis of ARGRI and ARGRIII regulatory proteins involved in the regulation of arginine metabolism in Saccharomyces cerevisiae. Mol Gen Genet. 1990;222:192-200 pubmed
    ..Gene disruption of the ARGRIII gene impairs the growth of the mutant on rich medium, showing that ARGRIII has a pleiotropic role in the cell. ..
  28. Van Camp W, Bowler C, Villarroel R, Tsang E, Van Montagu M, Inze D. Characterization of iron superoxide dismutase cDNAs from plants obtained by genetic complementation in Escherichia coli. Proc Natl Acad Sci U S A. 1990;87:9903-7 pubmed
    ..In addition, these results show the potential for shotgun cloning of eukaryotic genes by complementation of bacterial mutants, particularly when it is combined with a highly efficient transformation method, such as electroporation. ..
  29. Boonchird C, Messenguy F, Dubois E. Characterization of the yeast ARG5,6 gene: determination of the nucleotide sequence, analysis of the control region and of ARG5,6 transcript. Mol Gen Genet. 1991;226:154-66 pubmed
    ..Specific regulation involves two regions: one located between the putative TATA element and the transcriptional initiation site and the second between this site and the first ATG. ..
  30. van Rhijn P, Desair J, Vlassak K, Vanderleyden J. The NodD proteins of Rhizobium sp. strain BR816 differ in their interactions with coinducers and in their activities for nodulation of different host plants. Appl Environ Microbiol. 1994;60:3615-23 pubmed
    ..leucocephala, nodD3 and nodD4 appear to be important for the nodulation of Phaseolus vulgaris. It appears that NodD1 of BR816 can function as a transcriptional activator in bean nodulation but not in nodulation of L. leucocephala...
  31. Roovers M, Oudjama Y, Kaminska K, Purta E, Caillet J, Droogmans L, et al. Sequence-structure-function analysis of the bifunctional enzyme MnmC that catalyses the last two steps in the biosynthesis of hypermodified nucleoside mnm5s2U in tRNA. Proteins. 2008;71:2076-85 pubmed publisher
    ..Interestingly, the N-terminal domain is capable of independent folding, while the isolated C-terminal domain is incapable of folding on its own, a situation similar to the one reported recently for the rRNA modification enzyme RsmC. ..
  32. Den Herder J, Vanhee C, De Rycke R, Corich V, Holsters M, Goormachtig S. Nod factor perception during infection thread growth fine-tunes nodulation. Mol Plant Microbe Interact. 2007;20:129-37 pubmed
    ..However, local production of NFs by bacteria that are taken up by the plant cells at the stage of bacteroid formation is not required for correct symbiosome development...
  33. Sermon J, Wevers E, Jansen L, De Spiegeleer P, Vanoirbeek K, Aertsen A, et al. CorA affects tolerance of Escherichia coli and Salmonella enterica serovar Typhimurium to the lactoperoxidase enzyme system but not to other forms of oxidative stress. Appl Environ Microbiol. 2005;71:6515-23 pubmed
    ..These results indicate that CorA affects the lactoperoxidase sensitivity of E. coli by modulating the cytoplasmic concentrations of transition metals that enhance the toxicity of the lactoperoxidase system. ..
  34. Mathis R, Van Gijsegem F, De Rycke R, D Haeze W, Van Maelsaeke E, Anthonio E, et al. Lipopolysaccharides as a communication signal for progression of legume endosymbiosis. Proc Natl Acad Sci U S A. 2005;102:2655-60 pubmed
    ..Nod factors act first to trigger the onset of the nodulation and invasion program; LPSs inform the plant to proceed with the symbiotic interaction and to develop a functional fixation zone...
  35. Busch K, Martin B, Baniahmad A, Martial J, Renkawitz R, Muller M. Silencing subdomains of v-ErbA interact cooperatively with corepressors: involvement of helices 5/6. Mol Endocrinol. 2000;14:201-11 pubmed
    ..In conclusion, full silencing activity requires corepressor interaction with all three silencing subdomains, SSD1-3. Among these, SSD2 is a new target for N-CoR and SMRT and is essential for corepressor binding and function. ..
  36. Roovers M, Sanchez R, Legrain C, Glansdorff N. Experimental evolution of enzyme temperature activity profile: selection in vivo and characterization of low-temperature-adapted mutants of Pyrococcus furiosus ornithine carbamoyltransferase. J Bacteriol. 2001;183:1101-5 pubmed
    ..The mutants were double ones, still complementing at 15 degrees C, a temperature already in the psychrophilic range. Their kinetic analysis is reported. ..
  37. Hauwaerts D, Alexandre G, Das S, Vanderleyden J, Zhulin I. A major chemotaxis gene cluster in Azospirillum brasilense and relationships between chemotaxis operons in alpha-proteobacteria. FEMS Microbiol Lett. 2002;208:61-7 pubmed
    ..brasilense operon to one of them. Interestingly, operons that are shown to be major regulators of behavior in several alpha-proteobacterial species are not orthologous...
  38. Verbruggen N, Villarroel R, Van Montagu M. Osmoregulation of a pyrroline-5-carboxylate reductase gene in Arabidopsis thaliana. Plant Physiol. 1993;103:771-81 pubmed
    ..thaliana plants resulted in a 5-fold induction of the AT-P5C1 transcript, suggesting osmoregulation of the AT-P5C1 promoter region. Moreover, a time-course experiment indicated that this induction precedes proline accumulation. ..
  39. Torres Acosta J, de Almeida Engler J, Raes J, Magyar Z, De Groodt R, Inze D, et al. Molecular characterization of Arabidopsis PHO80-like proteins, a novel class of CDKA;1-interacting cyclins. Cell Mol Life Sci. 2004;61:1485-97 pubmed
    ..The possible role of CYCPs in linking cell division, cell differentiation, and the nutritional status of the cell is discussed. ..
  40. Terrak M, Ghosh T, van Heijenoort J, Van Beeumen J, Lampilas M, Aszodi J, et al. The catalytic, glycosyl transferase and acyl transferase modules of the cell wall peptidoglycan-polymerizing penicillin-binding protein 1b of Escherichia coli. Mol Microbiol. 1999;34:350-64 pubmed
    ..Attempts to produce truncated forms of the PBP lead to the conclusion that the multimodular polypeptide chain behaves as an integrated folding entity during PBP1b biogenesis. ..
  41. Van Damme P, Lasa M, Polevoda B, Gazquez C, Elosegui Artola A, Kim D, et al. N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB. Proc Natl Acad Sci U S A. 2012;109:12449-54 pubmed publisher