Genomes and Genes
Experts and Doctors on gene deletion in United Kingdom
Locale: United Kingdom
Topic: gene deletion
Publications122 found, 100 shown here
- Daniel R, Noirot Gros M, Noirot P, Errington J. Multiple interactions between the transmembrane division proteins of Bacillus subtilis and the role of FtsL instability in divisome assembly. J Bacteriol. 2006;188:7396-404 pubmed publisher..DivIC is unusual in interacting strongly only with FtsL. We propose a model for the formation of the OR through the mutual association of the membrane proteins directed by the cytosolic inner-ring proteins...
- Karunakaran R, Ebert K, Harvey S, Leonard M, Ramachandran V, Poole P. Thiamine is synthesized by a salvage pathway in Rhizobium leguminosarum bv. viciae strain 3841. J Bacteriol. 2006;188:6661-8 pubmed..Overall, the results show that a thiamine salvage pathway operates to enable growth of Rhizobium leguminosarum in the rhizosphere, allowing its survival when thiamine is limiting. ..
- Alzuherri H, McGilvray R, Kilbey A, Bartholomew C. Conservation and expression of a novel alternatively spliced Evi1 exon. Gene. 2006;384:154-62 pubmed
- Foulkes T, Nassar M, Lane T, Matthews E, Baker M, Gerke V, et al. Deletion of annexin 2 light chain p11 in nociceptors causes deficits in somatosensory coding and pain behavior. J Neurosci. 2006;26:10499-507 pubmed..A significant deficit in neuropathic pain behavior was also apparent in the conditional-null mice. These results confirm an important role for p11 in nociceptor function. ..
- Hunt S, Elvin M, Crosthwaite S, Heintzen C. The PAS/LOV protein VIVID controls temperature compensation of circadian clock phase and development in Neurospora crassa. Genes Dev. 2007;21:1964-74 pubmed..Thus, temperature compensation of clock-controlled output is a key factor in maintaining temperature compensation of the entire circadian system. ..
- Paul C, Povey J, Lawrence N, Selfridge J, Melton D, Saunders P. Deletion of genes implicated in protecting the integrity of male germ cells has differential effects on the incidence of DNA breaks and germ cell loss. PLoS ONE. 2007;2:e989 pubmed..These findings increase our understanding of the ways in which gene mutations can have an impact on male fertility. ..
- Hunt D, Saldanha J, Brennan J, Benjamin P, Strom M, Cole J, et al. Single nucleotide polymorphisms that cause structural changes in the cyclic AMP receptor protein transcriptional regulator of the tuberculosis vaccine strain Mycobacterium bovis BCG alter global gene expression without attenuating growth. Infect Immun. 2008;76:2227-34 pubmed publisher..However, they may have allowed BCG to grow better under the in vitro-selective conditions used in its derivation from the M. bovis wild type. ..
- Meyer M, Genkov T, Skepper J, Jouhet J, Mitchell M, Spreitzer R, et al. Rubisco small-subunit ?-helices control pyrenoid formation in Chlamydomonas. Proc Natl Acad Sci U S A. 2012;109:19474-9 pubmed publisher..With the algal CCM being functionally analogous to the terrestrial C(4) pathway in higher plants, such insights may offer a route toward transforming algal and higher plant productivity for the future. ..
- Higgins M, Oprian D, Schertler G. Recoverin binds exclusively to an amphipathic peptide at the N terminus of rhodopsin kinase, inhibiting rhodopsin phosphorylation without affecting catalytic activity of the kinase. J Biol Chem. 2006;281:19426-32 pubmed
- Speirs V, Adams I, Walton D, Atkin S. Identification of wild-type and exon 5 deletion variants of estrogen receptor beta in normal human mammary gland. J Clin Endocrinol Metab. 2000;85:1601-5 pubmed..07). In conclusion, our data would support a role for ERbeta in the normal human mammary gland, where we propose it may be the dominant receptor. ..
- Allers T, Ngo H, Mevarech M, Lloyd R. Development of additional selectable markers for the halophilic archaeon Haloferax volcanii based on the leuB and trpA genes. Appl Environ Microbiol. 2004;70:943-53 pubmed..We constructed both integrative and replicative plasmids carrying the leuB or trpA gene under control of a constitutive promoter. The use of these selectable markers in deletion of the lhr gene of H. volcanii is described. ..
- Hill K, Krugmann S, Andrews S, Coadwell W, Finan P, Welch H, et al. Regulation of P-Rex1 by phosphatidylinositol (3,4,5)-trisphosphate and Gbetagamma subunits. J Biol Chem. 2005;280:4166-73 pubmed
- Lawrence C, Botting C, Antrobus R, Coote P. Evidence of a new role for the high-osmolarity glycerol mitogen-activated protein kinase pathway in yeast: regulating adaptation to citric acid stress. Mol Cell Biol. 2004;24:3307-23 pubmed..Supporting this, Mdh1p and Idh1p, both TCA cycle enzymes, were up-regulated in response to citric acid. ..
- Kerr A, Paterson G, McCluskey J, Iannelli F, Oggioni M, Pozzi G, et al. The contribution of PspC to pneumococcal virulence varies between strains and is accomplished by both complement evasion and complement-independent mechanisms. Infect Immun. 2006;74:5319-24 pubmed..However, other functions of PspC also contribute to virulence, as demonstrated by the finding that the pspC-deficient TIGR4 mutant was still attenuated relative to the wild-type parent, even in the absence of C3. ..
- Mina J, Thye J, Alqaisi A, Bird L, Dods R, Grøftehauge M, et al. Functional and phylogenetic evidence of a bacterial origin for the first enzyme in sphingolipid biosynthesis in a phylum of eukaryotic protozoan parasites. J Biol Chem. 2017;292:12208-12219 pubmed publisher..Therefore this enzyme, conserved throughout the Apicomplexa, is likely to have been obtained via lateral gene transfer from a prokaryote. ..
- Hernando Y, Parr A, Schweizer M. PRS5, the fifth member of the phosphoribosyl pyrophosphate synthetase gene family in Saccharomyces cerevisiae, is essential for cell viability in the absence of either PRS1 or PRS3. J Bacteriol. 1998;180:6404-7 pubmed..However, simultaneous deletion of PRS1 and PRS5 or PRS3 and PRS5 rendered the strains inviable, which implies that PRS5 plays an important role in the maintenance of PRS function in S. cerevisiae. ..
- Yesilkaya H, Kadioglu A, Gingles N, Alexander J, Mitchell T, Andrew P. Role of manganese-containing superoxide dismutase in oxidative stress and virulence of Streptococcus pneumoniae. Infect Immun. 2000;68:2819-26 pubmed..After infection with D39HY1, neutrophils were densely packed around bronchioles, in contrast to the wild-type infection, where neutrophils were more diffusely localized. ..
- Jones R, Britt Compton B, Williams P. The naphthalene catabolic (nag) genes of Ralstonia sp. strain U2 are an operon that is regulated by NagR, a LysR-type transcriptional regulator. J Bacteriol. 2003;185:5847-53 pubmed..However, other mutations in the region increased the effectiveness of salicylate as an inducer. ..
- Lee S, Faux C, Nixon J, Alete D, Chilton J, Hawadle M, et al. Dimerization of protein tyrosine phosphatase sigma governs both ligand binding and isoform specificity. Mol Cell Biol. 2007;27:1795-808 pubmed..These findings have important implications for our understanding of how heterophilic ligands interact with, and potentially regulate, RPTPs. ..
- Kershaw M, Talbot N. Genome-wide functional analysis reveals that infection-associated fungal autophagy is necessary for rice blast disease. Proc Natl Acad Sci U S A. 2009;106:15967-72 pubmed publisher..A genome-wide analysis therefore demonstrates the importance of infection-associated, nonselective autophagy for the establishment of rice blast disease. ..
- Klyuch B, Dale N, Wall M. Deletion of ecto-5'-nucleotidase (CD73) reveals direct action potential-dependent adenosine release. J Neurosci. 2012;32:3842-7 pubmed publisher..Our findings are a major step in understanding the mechanisms of adenosine release and are likely to have implications for all aspects of physiology where adenosine plays a key modulatory role. ..
- Beesley A, Ortega B, White S. Splicing of a retained intron within ROMK K+ channel RNA generates a novel set of isoforms in rat kidney. Am J Physiol. 1999;276:C585-92 pubmed..The deletion in this core region is predicted to generate hydrophilic proteins that are approximately one-third of the size of native ROMK and lack membrane-spanning domains. ..
- Feldman B, Concha M, Saude L, Parsons M, Adams R, Wilson S, et al. Lefty antagonism of Squint is essential for normal gastrulation. Curr Biol. 2002;12:2129-35 pubmed..Furthermore, we find that Cyclops is specifically required for the maintenance of lefty1 and lefty2 transcription. ..
- Li S, Spooner R, Allen S, Guise C, Ladds G, Schnöder T, et al. Folding-competent and folding-defective forms of ricin A chain have different fates after retrotranslocation from the endoplasmic reticulum. Mol Biol Cell. 2010;21:2543-54 pubmed publisher..We conclude that cytosolic ERAD components, particularly the proteasome RP, can discriminate between structural features of the same substrate. ..
- Griffiths H, Whitehouse I, Baybutt H, Brown D, Kellett K, Jackson C, et al. Prion protein interacts with BACE1 protein and differentially regulates its activity toward wild type and Swedish mutant amyloid precursor protein. J Biol Chem. 2011;286:33489-500 pubmed publisher..Thus, although PrP(C) exerts no control on cleavage of APP(Swe) by BACE1, it has a profound influence on the cleavage of APP(WT), suggesting that PrP(C) may be a key protective player against sporadic Alzheimer disease. ..
- Chakraverty R, Kearsey J, Oakley T, Grenon M, de La Torre Ruiz M, Lowndes N, et al. Topoisomerase III acts upstream of Rad53p in the S-phase DNA damage checkpoint. Mol Cell Biol. 2001;21:7150-62 pubmed..These data implicate Top3p and, by inference, Sgs1p in an S-phase-specific role in the cellular response to DNA damage. A model proposing a role for these proteins in S phase is presented. ..
- Engwerda C, Mynott T, Sawhney S, de Souza J, Bickle Q, Kaye P. Locally up-regulated lymphotoxin alpha, not systemic tumor necrosis factor alpha, is the principle mediator of murine cerebral malaria. J Exp Med. 2002;195:1371-7 pubmed
- Macrae J, Obado S, Turnock D, Roper J, Kierans M, Kelly J, et al. The suppression of galactose metabolism in Trypanosoma cruzi epimastigotes causes changes in cell surface molecular architecture and cell morphology. Mol Biochem Parasitol. 2006;147:126-36 pubmed..The apparent haploid deficiency suggests that epimerase levels are close to limiting, at least in the epimastigote form, and might be exploited as a potential drug target. ..
- Craig T, Shimomura K, Holl R, Flanagan S, Ellard S, Ashcroft F. An in-frame deletion in Kir6.2 (KCNJ11) causing neonatal diabetes reveals a site of interaction between Kir6.2 and SUR1. J Clin Endocrinol Metab. 2009;94:2551-7 pubmed publisher..Our results show that residues 28-32 in the N terminus of Kir6.2 interact both physically and functionally with SUR1 and suggest that residues 196-288 of SUR1 are important in this interaction. ..
- Hartshorne R, Reardon C, Ross D, Nuester J, Clarke T, Gates A, et al. Characterization of an electron conduit between bacteria and the extracellular environment. Proc Natl Acad Sci U S A. 2009;106:22169-74 pubmed publisher..We have identified the MtrAB module in a range of bacterial phyla, suggesting that it is widely used in electron exchange with the extracellular environment. ..
- Wood S, Gleadle J, Pugh C, Hankinson O, Ratcliffe P. The role of the aryl hydrocarbon receptor nuclear translocator (ARNT) in hypoxic induction of gene expression. Studies in ARNT-deficient cells. J Biol Chem. 1996;271:15117-23 pubmed
- Page T, D Souza Z, Nakanishi S, Serikawa T, Pusey C, Aitman T, et al. Role of novel rat-specific Fc receptor in macrophage activation associated with crescentic glomerulonephritis. J Biol Chem. 2012;287:5710-9 pubmed publisher..These results suggest that Fcgr3-rs may act to inhibit Fcgr3-mediated signaling and phagocytosis and could be considered as a novel mechanism in the modulation of Fc receptor-mediated cell activation in autoimmune diseases. ..
- Swindell S, Benson K, Griffin H, Renault P, Ehrlich S, Gasson M. Genetic manipulation of the pathway for diacetyl metabolism in Lactococcus lactis. Appl Environ Microbiol. 1996;62:2641-3 pubmed..Overexpression of the cloned ilvBN genes (encoding an alpha-acetolactate synthase) in the aldB deletion strain produced even higher levels of alpha-acetolactate, acetoin, and diacetyl...
- Raghu P, Colley N, Webel R, James T, Hasan G, Danin M, et al. Normal phototransduction in Drosophila photoreceptors lacking an InsP(3) receptor gene. Mol Cell Neurosci. 2000;15:429-45 pubmed..These results indicate that InsP(3)R-mediated store depletion does not underlie TRP and TRPL activation in Drosophila photoreceptors. ..
- Win T, Stevenson A, Wang S. Fission yeast Cid12 has dual functions in chromosome segregation and checkpoint control. Mol Cell Biol. 2006;26:4435-47 pubmed..To account for the effects of Cid12 on various aspects of DNA metabolism, including chromosome segregation and the checkpoint control, we suggest that Cid12 has dual functions in RNAi silencing and regulating mRNA stability. ..
- Verhagen J, GabrysovÃ¡ L, Minaee S, Sabatos C, Anderson G, Sharpe A, et al. Enhanced selection of FoxP3+ T-regulatory cells protects CTLA-4-deficient mice from CNS autoimmune disease. Proc Natl Acad Sci U S A. 2009;106:3306-11 pubmed publisher..We conclude that CTLA-4 is not essential for the peripheral function of FoxP3(+) Treg cells but plays a pivotal role in their thymic selection. ..
- Beste D, Bonde B, Hawkins N, Ward J, Beale M, Noack S, et al. ¹³C metabolic flux analysis identifies an unusual route for pyruvate dissimilation in mycobacteria which requires isocitrate lyase and carbon dioxide fixation. PLoS Pathog. 2011;7:e1002091 pubmed publisher..This study also provides a platform for further studies into the metabolism of M. tuberculosis using ¹³C-MFA...
- Miller S, Ness L, Wood C, Fox B, Booth I. Identification of an ancillary protein, YabF, required for activity of the KefC glutathione-gated potassium efflux system in Escherichia coli. J Bacteriol. 2000;182:6536-40 pubmed..Deletion of yabF reduces KefC activity 10-fold, and supply of YabF in trans restores activity. IS2 and IS10R insertions in yabF can be isolated as suppressors of KefC activity consequent upon the V427A and D264A KefC mutations. ..
- Fushimi K, Troeberg L, Nakamura H, Lim N, Nagase H. Functional differences of the catalytic and non-catalytic domains in human ADAMTS-4 and ADAMTS-5 in aggrecanolytic activity. J Biol Chem. 2008;283:6706-16 pubmed..However, removal of the spacer domain from ADAMTS-4 greatly enhanced more general proteolytic activity against non-aggrecan substrates, e.g. E. coli-expressed interglobular domain, fibromodulin, and carboxymethylated transferrin. ..
- Atack J, Harvey P, Jones M, Kelly D. The Campylobacter jejuni thiol peroxidases Tpx and Bcp both contribute to aerotolerance and peroxide-mediated stress resistance but have distinct substrate specificities. J Bacteriol. 2008;190:5279-90 pubmed publisher..Taken together, the results suggest that Tpx and Bcp are partially redundant antioxidant enzymes that play an important role in protection of C. jejuni against oxygen-induced oxidative stress...
- Reeve A, Meagher M, Lax N, Simcox E, Hepplewhite P, Jaros E, et al. The impact of pathogenic mitochondrial DNA mutations on substantia nigra neurons. J Neurosci. 2013;33:10790-801 pubmed publisher..Our finding that the loss of SN neurons was only severe in patients with POLG mutations suggests that acquired mitochondrial defects may be less well tolerated by SN neurons than by inherited ones. ..
- M ller C, Conejero L, Spink N, Wand M, Bancroft G, Titball R. Role of RelA and SpoT in Burkholderia pseudomallei virulence and immunity. Infect Immun. 2012;80:3247-55 pubmed publisher..pseudomallei, and the ?relA ?spoT mutant may be a promising live-attenuated vaccine candidate...
- Beechey C. Peg1/Mest locates distal to the currently defined imprinting region on mouse proximal chromosome 6 and identifies a new imprinting region affecting growth. Cytogenet Cell Genet. 2000;90:309-14 pubmed..dist6 mice is compared to that of Mest-deficient mutant mice. Unlike the latter, MatDp.dist6 mice have good survival rates and females have normal maternal behaviour. Possible reasons for these differences are discussed. ..
- Venkateswarlu K. Interaction protein for cytohesin exchange factors 1 (IPCEF1) binds cytohesin 2 and modifies its activity. J Biol Chem. 2003;278:43460-9 pubmed..The functional significance of the IPCEF1-cytohesin 2 interaction is demonstrated by showing that IPCEF1 increases the in vitro and in vivo stimulation of ARFGTP formation by cytohesin 2. ..
- Stewart J, Silvia O, Atkin I, Hughes D, Ebrahimi B, Adler H. In vivo function of a gammaherpesvirus virion glycoprotein: influence on B-cell infection and mononucleosis. J Virol. 2004;78:10449-59 pubmed..Thus, gp150 is highly similar in function in vitro to the Epstein-Barr virus gp350/220. These results suggest a role for these analogous proteins in mononucleosis and have implications for their use as vaccine antigens. ..
- Kearney L, Gonzalez De Castro D, Yeung J, Procter J, Horsley S, Eguchi Ishimae M, et al. Specific JAK2 mutation (JAK2R683) and multiple gene deletions in Down syndrome acute lymphoblastic leukemia. Blood. 2009;113:646-8 pubmed publisher..These results infer a complex molecular pathogenesis for DS-ALL leukemogenesis, with trisomy 21 as an initiating or first hit and with chromosome aneuploidy, gene deletions, and activating JAK2 mutations as complementary genetic events. ..
- Hernando Y, Carter A, Parr A, Hove Jensen B, Schweizer M. Genetic analysis and enzyme activity suggest the existence of more than one minimal functional unit capable of synthesizing phosphoribosyl pyrophosphate in Saccharomyces cerevisiae. J Biol Chem. 1999;274:12480-7 pubmed..These data suggest that yeast phosphoribosyl pyrophosphate synthetase exists in vivo as multimeric complex(es). ..
- Skinner C, Rumsby G, Honour J. Single strand conformation polymorphism (SSCP) analysis for the detection of mutations in the CYP11B1 gene. J Clin Endocrinol Metab. 1996;81:2389-93 pubmed..Mutations were neither the result of gene conversion nor nonhomologous recombination between the two closely related CYP11B genes. ..
- Knight P, Wright S, Lawrence C, Paterson Y, Miller H. Delayed expulsion of the nematode Trichinella spiralis in mice lacking the mucosal mast cell-specific granule chymase, mouse mast cell protease-1. J Exp Med. 2000;192:1849-56 pubmed..These data demonstrate, for the first time, that the ablation of an MMC-derived effector molecule compromises the expulsion process. ..
- Roberts C, Sutherland H, Farmer H, Kimber W, Halford S, Carey A, et al. Targeted mutagenesis of the Hira gene results in gastrulation defects and patterning abnormalities of mesoendodermal derivatives prior to early embryonic lethality. Mol Cell Biol. 2002;22:2318-28 pubmed
- Cabart P, Murphy S. Assembly of human small nuclear RNA gene-specific transcription factor IIIB complex de novo on and off promoter. J Biol Chem. 2002;277:26831-8 pubmed..TBP.DNA complex, and subsequently stimulate pol III transcription. Moreover, mutation of the BURE reduces pol III transcription and induces transcription by RNA polymerase II from the U2 gene promoter carrying a minimal TATA box. ..
- Markiewicz E, Dechat T, Foisner R, Quinlan R, Hutchison C. Lamin A/C binding protein LAP2alpha is required for nuclear anchorage of retinoblastoma protein. Mol Biol Cell. 2002;13:4401-13 pubmed..Together, these data suggest that hypophosphorylated Rb is anchored in the nucleus by the interaction of pocket C with LAP2alpha-lamin A/C complexes. ..
- Coutinho P, Parsons M, Thomas K, Hirst E, Saude L, Campos I, et al. Differential requirements for COPI transport during vertebrate early development. Dev Cell. 2004;7:547-58 pubmed..Moreover, we note that the mRNA encoding alpha coatomer is strikingly upregulated in notochord progenitors, and we present data suggesting that alpha coatomer transcription is tuned to activity- and cell type-specific secretory loads. ..
- Bradbury D, Clarke D, Seedhouse C, Corbett L, Stocks J, Knox A. Vascular endothelial growth factor induction by prostaglandin E2 in human airway smooth muscle cells is mediated by E prostanoid EP2/EP4 receptors and SP-1 transcription factor binding sites. J Biol Chem. 2005;280:29993-30000 pubmed..These studies suggest that PGE2 increases VEGF transcriptionally and involves the Sp-1 binding site via a cAMP-dependent mechanism involving EP2 and EP4 receptors. ..
- McIntosh P, Martin S, Jackson D, Khan J, Isaacson E, Calder L, et al. Structural analysis reveals an amyloid form of the human papillomavirus type 16 E1--E4 protein and provides a molecular basis for its accumulation. J Virol. 2008;82:8196-203 pubmed publisher..Our results suggest a role for fibril formation in facilitating the accumulation of E1--E4 during HPV infection...
- Morgan M, Magnusdottir E, Kuo T, Tunyaplin C, Harper J, Arnold S, et al. Blimp-1/Prdm1 alternative promoter usage during mouse development and plasma cell differentiation. Mol Cell Biol. 2009;29:5813-27 pubmed publisher..Collectively, these experiments provide insight into the organization of the Prdm1 gene and demonstrate that NF-kappaB is a key mediator of Prdm1 expression. ..
- Goddard A, Stevens J, Rao F, Mavridou D, Chan W, Richardson D, et al. c-Type cytochrome biogenesis can occur via a natural Ccm system lacking CcmH, CcmG, and the heme-binding histidine of CcmE. J Biol Chem. 2010;285:22882-9 pubmed publisher..These observations call for reconsideration of the accepted models of System I-mediated c-type cytochrome biogenesis. ..
- Lucidarme J, Tan L, Exley R, Findlow J, Borrow R, Tang C. Characterization of Neisseria meningitidis isolates that do not express the virulence factor and vaccine antigen factor H binding protein. Clin Vaccine Immunol. 2011;18:1002-14 pubmed publisher..No fH binding was detected to other ligands among the fHbp-negative strains. The implications of these findings for meningococcal pathogenesis and prevention are discussed...
- Badaruddin M, Holcombe L, Wilson R, Wang Z, Kershaw M, Talbot N. Glycogen metabolic genes are involved in trehalose-6-phosphate synthase-mediated regulation of pathogenicity by the rice blast fungus Magnaporthe oryzae. PLoS Pathog. 2013;9:e1003604 pubmed publisher..oryzae. ..
- O Reilly V, Lopes Floro K, Shi H, Chapman B, Preis J, James A, et al. Gene-environment interaction demonstrates the vulnerability of the embryonic heart. Dev Biol. 2014;391:99-110 pubmed publisher..Importantly, these findings extend our understanding of the roles of Hif1Î± in cardiovascular development. ..
- Nilsen H, Rosewell I, Robins P, Skjelbred C, Andersen S, Slupphaug G, et al. Uracil-DNA glycosylase (UNG)-deficient mice reveal a primary role of the enzyme during DNA replication. Mol Cell. 2000;5:1059-65 pubmed..The nuclear UNG protein has apparently evolved a specialized role in mammalian cells counteracting U:A base pairs formed by use of dUTP during DNA synthesis. ..
- Martin A, Wadhams G, Shah D, Porter S, Mantotta J, Craig T, et al. CheR- and CheB-dependent chemosensory adaptation system of Rhodobacter sphaeroides. J Bacteriol. 2001;183:7135-44 pubmed publisher..Intriguingly, CheA(2), but not CheA(1), is able to phosphorylate CheB(1), suggesting that signaling through CheA(1) cannot initiate feedback receptor adaptation via CheB(1)-P...
- James R, Arends M, Plowman S, Brooks D, Miles C, West J, et al. K-ras proto-oncogene exhibits tumor suppressor activity as its absence promotes tumorigenesis in murine teratomas. Mol Cancer Res. 2003;1:820-5 pubmed..Thus, in view of evidence that tumors can derive from tissue stem cells and that tumors harbor "cancer stem cells," aberrant K-ras expression could promote neoplastic progression by increasing their capacity for self-renewal. ..
- McCormack M, Forster A, Drynan L, Pannell R, Rabbitts T. The LMO2 T-cell oncogene is activated via chromosomal translocations or retroviral insertion during gene therapy but has no mandatory role in normal T-cell development. Mol Cell Biol. 2003;23:9003-13 pubmed..We conclude that there is no mandatory role for LMO2 in lymphoid development, implying that its specific role in T-cell tumorigenesis results from a reprogramming of gene expression after enforced expression in T-cell precursors...
- Pemberton A, Knight P, Gamble J, Colledge W, Lee J, Pierce M, et al. Innate BALB/c enteric epithelial responses to Trichinella spiralis: inducible expression of a novel goblet cell lectin, intelectin-2, and its natural deletion in C57BL/10 mice. J Immunol. 2004;173:1894-901 pubmed..spiralis infection suggest that this novel lectin may serve a protective role in the innate immune response to parasite infection...
- de Napoles M, Mermoud J, Wakao R, Tang Y, Endoh M, Appanah R, et al. Polycomb group proteins Ring1A/B link ubiquitylation of histone H2A to heritable gene silencing and X inactivation. Dev Cell. 2004;7:663-76 pubmed..These observations link H2A ubiquitylation, X inactivation, and PRC1 PcG function, suggesting an unanticipated and novel mechanism for chromatin-mediated heritable gene silencing. ..
- Sergeant J, Taylor E, Palecek J, Fousteri M, Andrews E, Sweeney S, et al. Composition and architecture of the Schizosaccharomyces pombe Rad18 (Smc5-6) complex. Mol Cell Biol. 2005;25:172-84 pubmed..In initial steps to understand the architecture of the complex, we identified two subcomplexes containing Rad18-Spr18-Nse2 and Nse1-Nse3-Rad62. The subcomplexes are probably bridged by a weaker interaction between Nse2 and Nse3. ..
- Bloor A, Cranenburgh R. An efficient method of selectable marker gene excision by Xer recombination for gene replacement in bacterial chromosomes. Appl Environ Microbiol. 2006;72:2520-5 pubmed..The high recombination frequency makes the inclusion of a counter-selectable marker gene unnecessary. ..
- Tremelling M, Hancock L, Bredin F, Sharpstone D, Bingham S, Parkes M. Complex insertion/deletion polymorphism in NOD1 (CARD4) is not associated with inflammatory bowel disease susceptibility in East Anglia panel. Inflamm Bowel Dis. 2006;12:967-71 pubmed..The discrepancy with the earlier report may reflect a smaller effect size than previously predicted, a false-positive result in the index study, or population heterogeneity. ..
- Bédard J, Kubis S, Bimanadham S, Jarvis P. Functional similarity between the chloroplast translocon component, Tic40, and the human co-chaperone, Hsp70-interacting protein (Hip). J Biol Chem. 2007;282:21404-14 pubmed..Supporting this notion, yeast two-hybrid and bimolecular fluorescence complementation assays demonstrated the in vivo interaction of Tic40 with Tic110, a protein believed to recruit stromal chaperones to protein import sites. ..
- Lange U, Adams D, Lee C, Barton S, Schneider R, Bradley A, et al. Normal germ line establishment in mice carrying a deletion of the Ifitm/Fragilis gene family cluster. Mol Cell Biol. 2008;28:4688-96 pubmed publisher..Hence, contrary to previous reports, Ifitm genes are not essential for PGC migration. The Ifitm family is a striking example of a conserved gene cluster which appears to be functionally redundant during development. ..
- Lossi N, Manoli E, Forster A, Dajani R, Pape T, Freemont P, et al. The HsiB1C1 (TssB-TssC) complex of the Pseudomonas aeruginosa type VI secretion system forms a bacteriophage tail sheathlike structure. J Biol Chem. 2013;288:7536-48 pubmed publisher..In conclusion, we provide further structural and mechanistic insights into the T6SS and show that a phage sheathlike structure is likely to be a conserved element across all T6SSs...
- Lenassi E, Saihan Z, Bitner Glindzicz M, Webster A. The effect of the common c.2299delG mutation in USH2A on RNA splicing. Exp Eye Res. 2014;122:9-12 pubmed publisher..Here, we report an effect of the common c.2299delG mutation on splicing of exons 12 and 13 of USH2A. Future studies are expected to provide important insights into the contribution of this effect on the phenotype. ..
- Prins J, Todd J, Rodrigues N, Ghosh S, Hogarth P, Wicker L, et al. Linkage on chromosome 3 of autoimmune diabetes and defective Fc receptor for IgG in NOD mice. Science. 1993;260:695-8 pubmed..The development of congenic strains and the characterization of Mendelian traits that are specific to the disease phenotype demonstrate the feasibility of dissecting the pathophysiology of complex, non-Mendelian diseases. ..
- Raux E, Thermes C, Heathcote P, Rambach A, Warren M. A role for Salmonella typhimurium cbiK in cobalamin (vitamin B12) and siroheme biosynthesis. J Bacteriol. 1997;179:3202-12 pubmed..In comparison to CysG, CbiK appears to have a greater affinity for Co2+ than for Fe2+, and it is likely that cbiK encodes an enzyme whose primary role is that of a cobalt chelatase in corrin biosynthesis. ..
- Wittschieben B, Otero G, de Bizemont T, Fellows J, Erdjument Bromage H, Ohba R, et al. A novel histone acetyltransferase is an integral subunit of elongating RNA polymerase II holoenzyme. Mol Cell. 1999;4:123-8 pubmed..These results suggest a role for a novel, tightly RNAPII-associated HAT in transcription of DNA packaged in chromatin. ..
- Barbash D, Roote J, Ashburner M. The Drosophila melanogaster hybrid male rescue gene causes inviability in male and female species hybrids. Genetics. 2000;154:1747-71 pubmed..melanogaster mothers. Implications of our findings for understanding Haldane's rule-the observation that hybrid breakdown is often specific to the heterogametic sex-are also discussed. ..
- Magdalena J, Millard T, Etienne Manneville S, Launay S, Warwick H, Machesky L. Involvement of the Arp2/3 complex and Scar2 in Golgi polarity in scratch wound models. Mol Biol Cell. 2003;14:670-84 pubmed..Furthermore, in NIH 3T3 cells, Scar2 and the Arp2/3 complex appear to be involved in the establishment and maintenance of Golgi polarity during directed migration. ..
- Hinton H, Alessi D, Cantrell D. The serine kinase phosphoinositide-dependent kinase 1 (PDK1) regulates T cell development. Nat Immunol. 2004;5:539-45 pubmed..These studies show that AGC family kinases are essential for T cell development. ..
- Nassar M, Stirling L, Forlani G, Baker M, Matthews E, Dickenson A, et al. Nociceptor-specific gene deletion reveals a major role for Nav1.7 (PN1) in acute and inflammatory pain. Proc Natl Acad Sci U S A. 2004;101:12706-11 pubmed..7 in inflammatory pain. Nociceptor-specific gene ablation should prove useful in understanding the role of other broadly expressed genes in pain pathways. ..
- Carpenter A, Porter A. Construction, characterization, and complementation of a conditional-lethal DNA topoisomerase IIalpha mutant human cell line. Mol Biol Cell. 2004;15:5700-11 pubmed
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