Experts and Doctors on fungal gene expression regulation in Belgium


Locale: Belgium
Topic: fungal gene expression regulation

Top Publications

  1. Nailis H, Kucharíková S, Ricicova M, Van Dijck P, Deforce D, Nelis H, et al. Real-time PCR expression profiling of genes encoding potential virulence factors in Candida albicans biofilms: identification of model-dependent and -independent gene expression. BMC Microbiol. 2010;10:114 pubmed publisher
    ..Therefore, the need to use multiple model systems when studying the expression of genes encoding potential virulence factors in C. albicans biofilms is highlighted. ..
  2. Francois I, Bink A, Vandercappellen J, Ayscough K, Toulmay A, Schneiter R, et al. Membrane rafts are involved in intracellular miconazole accumulation in yeast cells. J Biol Chem. 2009;284:32680-5 pubmed publisher
    ..We further show that this primary role of membrane rafts in miconazole action consists of mediating intracellular accumulation of miconazole in yeast cells. ..
  3. Crabeel M, De Rijcke M, Seneca S, Heimberg H, Pfeiffer I, Matisova A. Further definition of the sequence and position requirements of the arginine control element that mediates repression and induction by arginine in Saccharomyces cerevisiae. Yeast. 1995;11:1367-80 pubmed
    ..The new data suggest that the arginine repressor might inhibit transcription in an active process. ..
  4. Donaton M, Holsbeeks I, Lagatie O, Van Zeebroeck G, Crauwels M, Winderickx J, et al. The Gap1 general amino acid permease acts as an amino acid sensor for activation of protein kinase A targets in the yeast Saccharomyces cerevisiae. Mol Microbiol. 2003;50:911-29 pubmed
  5. Fickers P, Fudalej F, Nicaud J, Destain J, Thonart P. Selection of new over-producing derivatives for the improvement of extracellular lipase production by the non-conventional yeast Yarrowia lipolytica. J Biotechnol. 2005;115:379-86 pubmed
    ..In addition this production system developed for the extracellular lipase could also be applied for other heterologous protein production since we have demonstrated that LgX64.81 is an interesting alternative host strain. ..
  6. Abdel Sater F, Iraqui I, Urrestarazu A, Andre B. The external amino acid signaling pathway promotes activation of Stp1 and Uga35/Dal81 transcription factors for induction of the AGP1 gene in Saccharomyces cerevisiae. Genetics. 2004;166:1727-39 pubmed
    ..Finally, the data show that the TOR pathway mediating global nitrogen control of transcription does not interfere with the external amino acid signaling pathway. ..
  7. Nailis H, Vandenbosch D, Deforce D, Nelis H, Coenye T. Transcriptional response to fluconazole and amphotericin B in Candida albicans biofilms. Res Microbiol. 2010;161:284-92 pubmed publisher
    ..albicans cells that might contribute to a resistant biofilm phenotype. ..
  8. Thevissen K, Yen W, Carmona Gutierrez D, Idkowiak Baldys J, Aerts A, Francois I, et al. Skn1 and Ipt1 negatively regulate autophagy in Saccharomyces cerevisiae. FEMS Microbiol Lett. 2010;303:163-8 pubmed publisher
    ..These data point to a negative regulation of autophagy by both Ipt1 and Skn1 in yeast, with a putative involvement of phytosphingosine in this process. ..
  9. Ricicova M, Kucharíková S, Tournu H, Hendrix J, Bujdakova H, Van Eldere J, et al. Candida albicans biofilm formation in a new in vivo rat model. Microbiology. 2010;156:909-919 pubmed publisher
    ..This model can be used to characterize the ability of antimicrobial agents to eliminate biofilms, and to evaluate the prophylactic effect of antifungal drugs and biomaterial coatings. ..

More Information


  1. Smets B, De Snijder P, Engelen K, Joossens E, Ghillebert R, Thevissen K, et al. Genome-wide expression analysis reveals TORC1-dependent and -independent functions of Sch9. FEMS Yeast Res. 2008;8:1276-88 pubmed publisher
  2. Georis I, Tate J, Cooper T, Dubois E. Tor pathway control of the nitrogen-responsive DAL5 gene bifurcates at the level of Gln3 and Gat1 regulation in Saccharomyces cerevisiae. J Biol Chem. 2008;283:8919-29 pubmed publisher
  3. Van Driessche B, Coddens S, Van Mullem V, Vandenhaute J. Glucose deprivation mediates interaction between CTDK-I and Snf1 in Saccharomyces cerevisiae. FEBS Lett. 2005;579:5318-24 pubmed
    ..Northern blot analysis suggested that Ctk1 and Snf1 act together in vivo to regulate GSY2. These findings support the view that Ctk1 interacts with Snf1 in a functional module involved in the cellular response to glucose limitation. ..
  4. Swinnen E, Rosseels J, Winderickx J. The minimum domain of Pho81 is not sufficient to control the Pho85-Rim15 effector branch involved in phosphate starvation-induced stress responses. Curr Genet. 2005;48:18-33 pubmed
  5. Thevissen K, Idkowiak Baldys J, Im Y, Takemoto J, Francois I, Ferket K, et al. SKN1, a novel plant defensin-sensitivity gene in Saccharomyces cerevisiae, is implicated in sphingolipid biosynthesis. FEBS Lett. 2005;579:1973-7 pubmed
    ..These results show that SKN1, together with IPT1, is involved in sphingolipid biosynthesis in S. cerevisiae. ..
  6. Talibi D, Grenson M, Andre B. Cis- and trans-acting elements determining induction of the genes of the gamma-aminobutyrate (GABA) utilization pathway in Saccharomyces cerevisiae. Nucleic Acids Res. 1995;23:550-7 pubmed
    ..We propose that tight coupling between the UASGABA and UASGATA elements enables the cell to integrate, according to its nitrogen status, the induced expression levels of UGA4. ..
  7. El Alami M, Feller A, Piérard A, Dubois E. The proper folding of a long C-terminal segment of the yeast Lys14p regulator is required for activation of LYS genes in response to the metabolic effector. Mol Microbiol. 2002;43:1629-39 pubmed
    ..Our results suggest that the proper folding of the two-thirds C-terminal portion of Lys14p is essential not only to activate transcription but also to modulate it according to alphaAASA concentration. ..
  8. Rep M, Proft M, Remize F, Tamas M, Serrano R, Thevelein J, et al. The Saccharomyces cerevisiae Sko1p transcription factor mediates HOG pathway-dependent osmotic regulation of a set of genes encoding enzymes implicated in protection from oxidative damage. Mol Microbiol. 2001;40:1067-83 pubmed
    ..The five Sko1p target genes described here are suitable models for studying the interplay between osmotic and oxidative responses at the molecular and physiological levels. ..
  9. Iraqui I, Vissers S, Andre B, Urrestarazu A. Transcriptional induction by aromatic amino acids in Saccharomyces cerevisiae. Mol Cell Biol. 1999;19:3360-71 pubmed
    ..Finally, we show that Gap1p, the general amino acid permease, and Wap1p (Ycl025p), a newly discovered inducible amino acid permease with broad specificity, are the main aromatic amino acid transporters for catabolic purposes. ..
  10. de Winde J, Crauwels M, Hohmann S, Thevelein J, Winderickx J. Differential requirement of the yeast sugar kinases for sugar sensing in establishing the catabolite-repressed state. Eur J Biochem. 1996;241:633-43 pubmed
    ..These results indicate that sugar sensing and establishment of catabolite repression are controlled by an interregulatory network, involving all three yeast sugar kinases and the Ras-cAMP pathway. ..
  11. Ramos F, Verhasselt P, Feller A, Peeters P, Wach A, Dubois E, et al. Identification of a gene encoding a homocitrate synthase isoenzyme of Saccharomyces cerevisiae. Yeast. 1996;12:1315-20 pubmed
    ..The N-terminal end of homocitrate synthase isoform coded by LYS20 contains no typical mitochondrial targeting sequence, suggesting that this enzyme is not located in the mitochondria. ..
  12. Desmyter L, Verstraelen J, Dewaele S, Libert C, Contreras R, Chen C. Nonclassical export pathway: overexpression of NCE102 reduces protein and DNA damage and prolongs lifespan in an SGS1 deficient Saccharomyces cerevisiae. Biogerontology. 2007;8:527-35 pubmed
    ..We therefore demonstrate that a nonclassical export pathway functions as an alternative clearance/detoxification pathway to eliminate damaged material, when the basic repair pathway is not sufficient. ..
  13. Feller A, Dubois E, Ramos F, Pierard A. Repression of the genes for lysine biosynthesis in Saccharomyces cerevisiae is caused by limitation of Lys14-dependent transcriptional activation. Mol Cell Biol. 1994;14:6411-8 pubmed
    ..Such findings suggest that activation by alpha-aminoadipate semialdehyde and the apparent repression by lysine are related mechanisms. Lysine possibly acts by limiting the supply of the coinducer, alpha-aminoadipate semialdehyde. ..
  14. Van Huffel C, Dubois E, Messenguy F. Cloning and sequencing of Schizosaccharomyces pombe car1 gene encoding arginase. Expression of the arginine anabolic and catabolic genes in response to arginine and related metabolites. Yeast. 1994;10:923-33 pubmed
    ..cerevisiae, and also indicates that in S. pombe enzyme compartmentation is not always sufficient to control the arginine metabolic flux. ..
  15. Georis I, Feller A, Vierendeels F, Dubois E. The yeast GATA factor Gat1 occupies a central position in nitrogen catabolite repression-sensitive gene activation. Mol Cell Biol. 2009;29:3803-15 pubmed publisher
    ..Dal80-dependent repression of NCR-sensitive gene expression occurred at three possible levels: Dal80 represses GAT1 expression, it competes with Gat1 for binding, and it directly represses NCR gene transcription. ..
  16. Soussi Boudekou S, Vissers S, Urrestarazu A, Jauniaux J, Andre B. Gzf3p, a fourth GATA factor involved in nitrogen-regulated transcription in Saccharomyces cerevisiae. Mol Microbiol. 1997;23:1157-68 pubmed
    ..In addition, both Uga43p and Gzf3p moderately regulate expression of their own genes. These two proteins seem to be parts of a complex network of GATA factors which probably play a determining role in nitrogen-regulated transcription. ..
  17. Iraqui I, Vissers S, Cartiaux M, Urrestarazu A. Characterisation of Saccharomyces cerevisiae ARO8 and ARO9 genes encoding aromatic aminotransferases I and II reveals a new aminotransferase subfamily. Mol Gen Genet. 1998;257:238-48 pubmed
    ..An autonomously replicating sequence (ARS) element is located between the ARO8 gene and YGL201c which encodes a protein of the minichromosome maintenance family. ..
  18. El Alami M, Messenguy F, Scherens B, Dubois E. Arg82p is a bifunctional protein whose inositol polyphosphate kinase activity is essential for nitrogen and PHO gene expression but not for Mcm1p chaperoning in yeast. Mol Microbiol. 2003;49:457-68 pubmed
    ..Our results indicate that Arg82p is a bifunctional protein whose inositol kinase activity plays a role in multiple signalling cascades, and whose acidic domain protects two MADS-box proteins against degradation. ..
  19. Iraqui I, Vissers S, Bernard F, De Craene J, Boles E, Urrestarazu A, et al. Amino acid signaling in Saccharomyces cerevisiae: a permease-like sensor of external amino acids and F-Box protein Grr1p are required for transcriptional induction of the AGP1 gene, which encodes a broad-specificity amino acid permease. Mol Cell Biol. 1999;19:989-1001 pubmed
    ..The essential role of Grr1p in this amino acid signaling pathway lends further support to the hypothesis that this protein participates in integrating nutrient availability with the cell cycle. ..
  20. Roosen J, Engelen K, Marchal K, Mathys J, Griffioen G, Cameroni E, et al. PKA and Sch9 control a molecular switch important for the proper adaptation to nutrient availability. Mol Microbiol. 2005;55:862-80 pubmed
    ..This mechanism allows fine-tuning various nutritional responses in yeast cells, allowing them to adapt metabolism and growth appropriately. ..
  21. Velasco I, Tenreiro S, Calderon I, Andre B. Saccharomyces cerevisiae Aqr1 is an internal-membrane transporter involved in excretion of amino acids. Eukaryot Cell. 2004;3:1492-503 pubmed
    ..We propose that Aqr1 catalyzes transport of excess amino acids into vesicles, which then release them in the extracellular space by exocytosis. ..
  22. Boonchird C, Messenguy F, Dubois E. Characterization of the yeast ARG5,6 gene: determination of the nucleotide sequence, analysis of the control region and of ARG5,6 transcript. Mol Gen Genet. 1991;226:154-66 pubmed
    ..Specific regulation involves two regions: one located between the putative TATA element and the transcriptional initiation site and the second between this site and the first ATG. ..