Experts and Doctors on flavoproteins in United States


Locale: United States
Topic: flavoproteins

Top Publications

  1. Bijur G, Jope R. Proapoptotic stimuli induce nuclear accumulation of glycogen synthase kinase-3 beta. J Biol Chem. 2001;276:37436-42 pubmed
    ..Thus, the intracellular distribution of GSK-3 beta is dynamically regulated by signaling cascades, and apoptotic stimuli cause increased nuclear levels of GSK-3 beta, which facilitates interactions with nuclear substrates. ..
  2. Dufour M, Yan C, Siegel D, Colucci M, Jenner M, Oldham N, et al. Mechanism-based inhibition of quinone reductase 2 (NQO2): selectivity for NQO2 over NQO1 and structural basis for flavoprotein inhibition. Chembiochem. 2011;12:1203-8 pubmed publisher
  3. Kao C, Kittleman W, Zhang H, Seto H, Liu H. Stereochemical analysis of isopentenyl diphosphate isomerase type II from Staphylococcus aureus using chemically synthesized (S)- and (R)-[2-2H]isopentenyl diphosphates. Org Lett. 2005;7:5677-80 pubmed
    ..Our results show that the C-2 deprotonation of IPP by this enzyme is pro-R stereospecific, suggesting a similar stereochemical course as the type I enzyme. ..
  4. Kao Y, Tan C, Song S, Ozturk N, Li J, Wang L, et al. Ultrafast dynamics and anionic active states of the flavin cofactor in cryptochrome and photolyase. J Am Chem Soc. 2008;130:7695-701 pubmed publisher
    ..These unique properties of anionic flavins suggest a universal mechanism of electron transfer for the initial functional steps of the photolyase/cryptochrome blue-light photoreceptor family. ..
  5. Lemos D, Downs J, Urbanski H. Twenty-four-hour rhythmic gene expression in the rhesus macaque adrenal gland. Mol Endocrinol. 2006;20:1164-76 pubmed
    ..Moreover, they strongly imply the existence of an intrinsic circadian clock. ..
  6. Zhu H, Riggs A. Yeast flavohemoglobin is an ancient protein related to globins and a reductase family. Proc Natl Acad Sci U S A. 1992;89:5015-9 pubmed
    ..8 billion years, the estimated time of divergence of prokaryotes and eukaryotes provided that cross-species gene transfer has not occurred. ..
  7. Saxena C, Wang H, Kavakli I, Sancar A, Zhong D. Ultrafast dynamics of resonance energy transfer in cryptochrome. J Am Chem Soc. 2005;127:7984-5 pubmed
    ..The results for the first time show energy transfer in cryptochrome suggesting some mechanistic similarities between photolyase that repairs damaged DNA and cryptochrome that mediates blue-light signaling. ..
  8. Veluchamy S, Rollins J. A CRY-DASH-type photolyase/cryptochrome from Sclerotinia sclerotiorum mediates minor UV-A-specific effects on development. Fungal Genet Biol. 2008;45:1265-76 pubmed publisher
    ..This study suggests that cry1 may have a function during UV exposure but is not essential for completing the developmental life cycle under laboratory conditions. ..
  9. Lucas Lledó J, Lynch M. Evolution of mutation rates: phylogenomic analysis of the photolyase/cryptochrome family. Mol Biol Evol. 2009;26:1143-53 pubmed publisher
    ..In contrast, the loss of photolyase genes in prokaryotes may not cause an increase in the mutation rate and be neutral in most cases. ..

More Information


  1. Ding Y, Ferry J. Flavin mononucleotide-binding flavoprotein family in the domain Archaea. J Bacteriol. 2004;186:90-7 pubmed
    ..A search of the databases suggests that AfpA is the prototype of a previously unrecognized flavoprotein family unique to the domain Archaea for which the name archaeoflavoprotein is proposed. ..
  2. Zima A, Kockskamper J, Mejia Alvarez R, Blatter L. Pyruvate modulates cardiac sarcoplasmic reticulum Ca2+ release in rats via mitochondria-dependent and -independent mechanisms. J Physiol. 2003;550:765-83 pubmed
    ..The latter effect was most probably mediated by an enhanced SR Ca2+ uptake due to an augmentation of mitochondria-dependent ATP synthesis. ..
  3. Olucha J, Lamb A. Mechanistic and structural studies of the N-hydroxylating flavoprotein monooxygenases. Bioorg Chem. 2011;39:171-7 pubmed publisher
    ..This includes summaries of steady and transient state kinetic data for all three enzymes and the X-ray crystallographic structure of PvdA. ..
  4. Sribnick E, Samantaray S, Das A, Smith J, Matzelle D, Ray S, et al. Postinjury estrogen treatment of chronic spinal cord injury improves locomotor function in rats. J Neurosci Res. 2010;88:1738-50 pubmed publisher
    ..These findings suggest that estrogen may be useful as a promising therapeutic agent for prevention of damage and improvement of locomotor function in chronic SCI. (c) 2010 Wiley-Liss, Inc. ..
  5. Altose M, Zheng Y, Dong J, Palfey B, Carey P. Comparing protein-ligand interactions in solution and single crystals by Raman spectroscopy. Proc Natl Acad Sci U S A. 2001;98:3006-11 pubmed
    ..In general, the ability to compare detailed Raman data for complexes in crystals and solution provides a means of bridging crystallographic and solution studies. ..
  6. Suharti S, Murakami K, de Vries S, Ferry J. Structural and biochemical characterization of flavoredoxin from the archaeon Methanosarcina acetivorans. Biochemistry. 2008;47:11528-35 pubmed publisher
    ..The FMN is bound with hydrogen bonds to the isoalloxazine ring and electrostatic interactions with the phosphate moiety that, together with sequence analyses of homologues, indicate a novel FMN binding motif for the flavoredoxin family. ..
  7. Wu G, Spalding E. Separate functions for nuclear and cytoplasmic cryptochrome 1 during photomorphogenesis of Arabidopsis seedlings. Proc Natl Acad Sci U S A. 2007;104:18813-8 pubmed
    ..An important step toward elucidation of cry1 signaling pathways is the recognition that different subcellular pools of the photoreceptor have different functions. ..
  8. Dragnea V, Arunkumar A, Lee C, Giedroc D, Bauer C. A Q63E Rhodobacter sphaeroides AppA BLUF domain mutant is locked in a pseudo-light-excited signaling state. Biochemistry. 2010;49:10682-90 pubmed publisher
    ..The significance of these findings on the mechanism of light-induced output signaling is discussed...
  9. Kondratov R, Shamanna R, Kondratova A, Gorbacheva V, Antoch M. Dual role of the CLOCK/BMAL1 circadian complex in transcriptional regulation. FASEB J. 2006;20:530-2 pubmed
  10. Dragnea V, Waegele M, Balascuta S, Bauer C, Dragnea B. Time-resolved spectroscopic studies of the AppA blue-light receptor BLUF domain from Rhodobacter sphaeroides. Biochemistry. 2005;44:15978-85 pubmed publisher
    ..Dynamics of the AppA photocycle is discussed in view of the currently solved crystallographic structure of AppA...
  11. Pedmale U, Liscum E. Regulation of phototropic signaling in Arabidopsis via phosphorylation state changes in the phototropin 1-interacting protein NPH3. J Biol Chem. 2007;282:19992-20001 pubmed
  12. Castillon A, Shen H, Huq E. Blue light induces degradation of the negative regulator phytochrome interacting factor 1 to promote photomorphogenic development of Arabidopsis seedlings. Genetics. 2009;182:161-71 pubmed publisher
  13. Kodali V, Thorpe C. Oxidative protein folding and the Quiescin-sulfhydryl oxidase family of flavoproteins. Antioxid Redox Signal. 2010;13:1217-30 pubmed publisher
    ..Finally, limitations of our current understanding of disulfide generation in metazoans are identified and questions posed for the future. ..
  14. Barquera B, Ramirez Silva L, Morgan J, Nilges M. A new flavin radical signal in the Na(+)-pumping NADH:quinone oxidoreductase from Vibrio cholerae. An EPR/electron nuclear double resonance investigation of the role of the covalently bound flavins in subunits B and C. J Biol Chem. 2006;281:36482-91 pubmed
    ..The second anionic radical has not been reported previously. In electron nuclear double resonance spectra, it exhibits a larger line width and larger 8alpha-methyl proton splittings, compared with the first anionic radical. ..
  15. Kondratov R, Kondratova A, Lee C, Gorbacheva V, Chernov M, Antoch M. Post-translational regulation of circadian transcriptional CLOCK(NPAS2)/BMAL1 complex by CRYPTOCHROMES. Cell Cycle. 2006;5:890-5 pubmed
  16. Dragnea V, Arunkumar A, Yuan H, Giedroc D, Bauer C. Spectroscopic studies of the AppA BLUF domain from Rhodobacter sphaeroides: addressing movement of tryptophan 104 in the signaling state. Biochemistry. 2009;48:9969-79 pubmed publisher
    ..These results do not support a model of Trp104 movement as a key output signal...
  17. Ozols J. Liver microsomes contain two distinct NADPH-Monooxygenases with NH2-terminal segments homologous to the flavin containing NADPH-monooxygenase of Pseudomonas fluorescens. Biochem Biophys Res Commun. 1989;163:49-55 pubmed
    ..These results indicate that the NADPH-monooxygenase system consists of at least two distinct proteins representing different gene products. ..
  18. Carothers D, Pons G, Patel M. Dihydrolipoamide dehydrogenase: functional similarities and divergent evolution of the pyridine nucleotide-disulfide oxidoreductases. Arch Biochem Biophys. 1989;268:409-25 pubmed
    ..Additionally, the sequence information may help to better understand a divergent evolutionary relationship among these flavoproteins in different species. ..
  19. Strittmatter P, Kittler J, Coghill J, Ozols J. Characterization of lysyl residues of NADH-cytochrome b5 reductase implicated in charge-pairing with active-site carboxyl residues of cytochrome b5 by site-directed mutagenesis of an expression vector for the flavoprotein. J Biol Chem. 1992;267:2519-23 pubmed
  20. Farrell S, Thorpe C. Augmenter of liver regeneration: a flavin-dependent sulfhydryl oxidase with cytochrome c reductase activity. Biochemistry. 2005;44:1532-41 pubmed
    ..These data suggest that this poorly understood flavoenzyme may not function as a sulfhydryl oxidase within the mitochondrial intermembrane space but may communicate with the respiratory chain via the mediation of cytochrome c...
  21. Yuan Z, Taatjes D, Mossman B, Heintz N. The duration of nuclear extracellular signal-regulated kinase 1 and 2 signaling during cell cycle reentry distinguishes proliferation from apoptosis in response to asbestos. Cancer Res. 2004;64:6530-6 pubmed
  22. Dirks A, Leeuwenburgh C. Aging and lifelong calorie restriction result in adaptations of skeletal muscle apoptosis repressor, apoptosis-inducing factor, X-linked inhibitor of apoptosis, caspase-3, and caspase-12. Free Radic Biol Med. 2004;36:27-39 pubmed
    ..Calorie restriction is able to lower the apoptotic potential in aged skeletal muscle by altering several key apoptotic proteins toward cellular survival, thereby reducing the potential for sarcopenia. ..
  23. Whippo C, Hangarter R. Second positive phototropism results from coordinated co-action of the phototropins and cryptochromes. Plant Physiol. 2003;132:1499-507 pubmed
    ..Based on our results, we hypothesize that phototropins and cryptochromes regulate phototropism by coordinating the balance between stimulation and inhibition of growth of the hypocotyl depending on the fluence rate of blue light. ..
  24. Kiss J, Mullen J, Correll M, Hangarter R. Phytochromes A and B mediate red-light-induced positive phototropism in roots. Plant Physiol. 2003;131:1411-7 pubmed
    ..Although phytochrome has been shown to mediate phototropism in some lower plant groups, this is one of the few reports indicating a phytochrome-dependent phototropism in flowering plants. ..
  25. Spalding E. Ion channels and the transduction of light signals. Plant Cell Environ. 2000;23:665-74 pubmed
    ..Also included is some discussion of the technical aspects of studies that combine electrophysiology and photobiology...
  26. Krey G, Vanin E, Swenson R. Cloning, nucleotide sequence, and expression of the flavodoxin gene from Desulfovibrio vulgaris (Hildenborough). J Biol Chem. 1988;263:15436-43 pubmed
    ..vulgaris. To our knowledge, this is the first example of the expression of a foreign flavodoxin gene in E. coli using recombinant DNA methods. ..
  27. Jang M, Scrutton N, Hille R. Formation of W(3)A(1) electron-transferring flavoprotein (ETF) hydroquinone in the trimethylamine dehydrogenase x ETF protein complex. J Biol Chem. 2000;275:12546-52 pubmed
  28. N lling J, Ishii M, Koch J, Pihl T, Reeve J, Thauer R, et al. Characterization of a 45-kDa flavoprotein and evidence for a rubredoxin, two proteins that could participate in electron transport from H2 to CO2 in methanogenesis in Methanobacterium thermoautotrophicum. Eur J Biochem. 1995;231:628-38 pubmed
    ..abstract truncated at 400 words)..
  29. Daithankar V, Wang W, Trujillo J, Thorpe C. Flavin-linked Erv-family sulfhydryl oxidases release superoxide anion during catalytic turnover. Biochemistry. 2012;51:265-72 pubmed publisher
    ..Aspects of the mechanism and possible physiological significance of superoxide release from these Erv-domain flavoproteins are discussed. ..
  30. Ye B, Kroboth S, Pu J, Sims J, Aggarwal N, McNally E, et al. Molecular identification and functional characterization of a mitochondrial sulfonylurea receptor 2 splice variant generated by intraexonic splicing. Circ Res. 2009;105:1083-93 pubmed publisher
    ..Cardioprotective pathways may involve a mitochondrial ATP-sensitive potassium (mitoK(ATP)) channel but its composition is not fully understood...
  31. Jin H, Zhang Y, Buchko G, Varnum S, Robinson H, Squier T, et al. Structure determination and functional analysis of a chromate reductase from Gluconacetobacter hansenii. PLoS ONE. 2012;7:e42432 pubmed publisher
    ..The proposed proximity relationships between metal anion binding site and enzyme cofactors is discussed in terms of rational design principles for the use of enzymes in chromate and uranyl bioremediation. ..
  32. Zhang M, Wang L, Zhong D. Photolyase: Dynamics and electron-transfer mechanisms of DNA repair. Arch Biochem Biophys. 2017;632:158-174 pubmed publisher
    ..For 6-4 photoproduct repair, a similar cyclic ET mechanism operates and a new cyclic proton transfer with a conserved histidine residue at the active site of (6-4) photolyases is revealed. ..
  33. Orville A, Lountos G, Finnegan S, Gadda G, Prabhakar R. Crystallographic, spectroscopic, and computational analysis of a flavin C4a-oxygen adduct in choline oxidase. Biochemistry. 2009;48:720-8 pubmed publisher
    ..We propose that the enzyme-bound FAD is reduced in the X-ray beam. The aerobic crystals then form either a C4a-OH or C4a-OO(H) adduct, but an insufficient proton inventory prevents their decay at cryogenic temperatures. ..
  34. Backiel J, Juárez O, Zagorevski D, Wang Z, Nilges M, Barquera B. Covalent binding of flavins to RnfG and RnfD in the Rnf complex from Vibrio cholerae. Biochemistry. 2008;47:11273-84 pubmed publisher
    ..In contrast, in NqrC and NqrB the flavins are located in a cytoplasmic loop. This topological analysis suggests that there may be mechanistic differences between the Rnf and Na+-NQR complexes. ..
  35. Wesley U, Bove P, Hristova M, McCarthy S, van Der Vliet A. Airway epithelial cell migration and wound repair by ATP-mediated activation of dual oxidase 1. J Biol Chem. 2007;282:3213-20 pubmed
  36. Larson J, Jenkins J, Schuermann J, Zhou Y, Becker D, Tanner J. Crystal structures of the DNA-binding domain of Escherichia coli proline utilization A flavoprotein and analysis of the role of Lys9 in DNA recognition. Protein Sci. 2006;15:2630-41 pubmed
    ..These results provide new insights into the structural basis of DNA recognition by PutA and reveal three-dimensional structural details of the PutA dimer interface. ..
  37. Silaghi Dumitrescu R, Kurtz D, Ljungdahl L, Lanzilotta W. X-ray crystal structures of Moorella thermoacetica FprA. Novel diiron site structure and mechanistic insights into a scavenging nitric oxide reductase. Biochemistry. 2005;44:6492-501 pubmed publisher
    ..These results provide new insight into the structural features that fine-tune biological non-heme diiron sites for dioxygen activation vs nitric oxide reduction...
  38. Silaghi Dumitrescu R, Coulter E, Das A, Ljungdahl L, Jameson G, Huynh B, et al. A flavodiiron protein and high molecular weight rubredoxin from Moorella thermoacetica with nitric oxide reductase activity. Biochemistry. 2003;42:2806-15 pubmed
    ..The accumulated results indicate that Hrb/FprA is fully capable of functioning in nitrosative stress protection in M. thermoacetica. ..
  39. Masuda S, Bauer C. AppA is a blue light photoreceptor that antirepresses photosynthesis gene expression in Rhodobacter sphaeroides. Cell. 2002;110:613-23 pubmed
    ..These results establish AppA as a transcription factor that controls both redox and blue light repression of photosystem gene expression by mediating DNA binding activity of PpsR. ..
  40. Das A, Coulter E, Kurtz D, Ljungdahl L. Five-gene cluster in Clostridium thermoaceticum consisting of two divergent operons encoding rubredoxin oxidoreductase- rubredoxin and rubrerythrin-type A flavoprotein- high-molecular-weight rubredoxin. J Bacteriol. 2001;183:1560-7 pubmed publisher
    ..Since Rbr and Rbo have been recently implicated in oxidative stress protection in several anaerobic bacteria and archaea, we suggest a similar function of these proteins in oxygen tolerance of C. thermoaceticum...
  41. Guo W, Ziegler D. Estimation of flavin-containing monooxygenase activities in crude tissue preparations by thiourea-dependent oxidation of thiocholine. Anal Biochem. 1991;198:143-8 pubmed
    ..In all tissues where both can be measured, rates of thiocholine oxidation and N,N-dimethylaniline N-oxygenation were virtually identical, which suggests that both activities are catalyzed by the same monooxygenase...
  42. Yin L, Dragnea V, Feldman G, Hammad L, Karty J, Dann C, et al. Redox and light control the heme-sensing activity of AppA. MBio. 2013;4:e00563-13 pubmed publisher
    ..Since AppA interacts with PpsR only in the dark, bound heme thus stimulates the antirepressor activity of PpsR. This causes the redirection of tetrapyrrole biosynthesis away from heme into the bacteriochlorophyll branch...
  43. Hoober K, Joneja B, White H, Thorpe C. A sulfhydryl oxidase from chicken egg white. J Biol Chem. 1996;271:30510-6 pubmed
    ..The close functional resemblance of the oxidase to the pyridine nucleotide-dependent disulfide oxidoreductase family is discussed. ..