Genomes and Genes
Experts and Doctors on escherichia coli proteins in United States
Locale: United States
Topic: escherichia coli proteins
Publications378 found, 100 shown here
- Saecker R, Record M. Protein surface salt bridges and paths for DNA wrapping. Curr Opin Struct Biol. 2002;12:311-9 pubmed
- Wang Y, Bruckner R, Stein R. Regulation of signal peptidase by phospholipids in membrane: characterization of phospholipid bilayer incorporated Escherichia coli signal peptidase. Biochemistry. 2004;43:265-70 pubmed..Accordingly, a kinetic analysis is presented to explain some of the unique features of phospholipid vesicles incorporated signal peptidase, including the effect of lipid concentration and substrate-vesicle interaction. ..
- Ma L, King G, Rothfield L. Positioning of the MinE binding site on the MinD surface suggests a plausible mechanism for activation of the Escherichia coli MinD ATPase during division site selection. Mol Microbiol. 2004;54:99-108 pubmed
- Layton J, Foster P. Error-prone DNA polymerase IV is regulated by the heat shock chaperone GroE in Escherichia coli. J Bacteriol. 2005;187:449-57 pubmed..We were unable to show that GroE interacts directly with Pol IV, suggesting that GroE may act indirectly. Together with previous results, these findings indicate that Pol IV is a component of several cellular stress responses. ..
- Segatori L, Murphy L, Arredondo S, Kadokura H, Gilbert H, Beckwith J, et al. Conserved role of the linker alpha-helix of the bacterial disulfide isomerase DsbC in the avoidance of misoxidation by DsbB. J Biol Chem. 2006;281:4911-9 pubmed
- Eun Y, Kurt N, Sekhar A, Cavagnero S. Thermodynamic and kinetic characterization of apoHmpH, a fast-folding bacterial globin. J Mol Biol. 2008;376:879-97 pubmed publisher..This study supports the view that the general folding features of bacterial and eukaryotic globins are preserved through evolution while kinetic details differ. ..
- Wang X, Zhao X. Contribution of oxidative damage to antimicrobial lethality. Antimicrob Agents Chemother. 2009;53:1395-402 pubmed publisher..Hydroxyl radicals then enhance antimicrobial lethality, as suggested by earlier work. Such findings indicate that oxidative stress networks may provide targets for antimicrobial potentiation...
- Walker C, Shi X, Sanderson M, Sargeant J, Nagaraja T. Prevalence of Escherichia coli O157:H7 in gut contents of beef cattle at slaughter. Foodborne Pathog Dis. 2010;7:249-55 pubmed publisher..The hindgut was the major site of prevalence of E. coli O157:H7 in cattle, a majority of the isolates within the same animal were clonally similar, and acid tolerance of hindgut isolates were not different from that of ruminal isolates. ..
- Storvik K, Foster P. The SMC-like protein complex SbcCD enhances DNA polymerase IV-dependent spontaneous mutation in Escherichia coli. J Bacteriol. 2011;193:660-9 pubmed publisher..By initiating or participating in double-strand break repair, SbcCD may provide DNA substrates for Pol IV polymerase activity. ..
- Pannuri A, Yakhnin H, Vakulskas C, Edwards A, Babitzke P, Romeo T. Translational repression of NhaR, a novel pathway for multi-tier regulation of biofilm circuitry by CsrA. J Bacteriol. 2012;194:79-89 pubmed publisher..We conclude that CsrA regulates the horizontally acquired pgaABCD operon and PGA biosynthesis at multiple levels. Furthermore, nhaR repression exemplifies an expanding role for CsrA as a global regulator of stress response systems. ..
- Sun H, Ruszczycky M, Chang W, Thibodeaux C, Liu H. Nucleophilic participation of reduced flavin coenzyme in mechanism of UDP-galactopyranose mutase. J Biol Chem. 2012;287:4602-8 pubmed publisher..4 ± 0.4. Together, these findings are most consistent with a chemical mechanism for UGM involving an S(N)2-type displacement of UDP from UDP-Galf/p by N5 of FAD(red). ..
- Ma L, Payne S. AhpC is required for optimal production of enterobactin by Escherichia coli. J Bacteriol. 2012;194:6748-57 pubmed publisher..These data suggested that AhpC affected the activity of EntC or the availability of the chorismate substrate...
- Zhong M, Ferrell B, Lu W, Chai Q, Wei Y. Insights into the function and structural flexibility of the periplasmic molecular chaperone SurA. J Bacteriol. 2013;195:1061-7 pubmed publisher..The formation of disulfide bond in these mutants has no observable detrimental effect on protein activity, indicating that SurA does not undergo large-scale conformational change while performing its function...
- Butler E, Davis R, Bari V, Nicholson P, Ruiz N. Structure-function analysis of MurJ reveals a solvent-exposed cavity containing residues essential for peptidoglycan biogenesis in Escherichia coli. J Bacteriol. 2013;195:4639-49 pubmed publisher..Together, our studies support the structural homology of MurJ to MOP exporter proteins, suggesting that MurJ might function as an essential transporter in PG biosynthesis...
- Ahmadi M, Fawaz S, Fang L, Yu Z, Pfeifer B. Molecular variation of the nonribosomal peptide-polyketide siderophore yersiniabactin through biosynthetic and metabolic engineering. Biotechnol Bioeng. 2016;113:1067-74 pubmed publisher..A final series of experiments enhanced endogenous anthranilate towards complete pathway formation of the associated analog which showed a selective ability to bind gold. ..
- Kisiela D, Radey M, Paul S, Porter S, Polukhina K, Tchesnokova V, et al. Inactivation of Transcriptional Regulators during Within-Household Evolution of Escherichia coli. J Bacteriol. 2017;199: pubmed publisher..Our findings help in understanding the adaptive dynamics and strategies of E. coli during short-term natural evolution. ..
- Wang P, Amato N, Wang Y. Cytotoxic and Mutagenic Properties of C3'-Epimeric Lesions of 2'-Deoxyribonucleosides in Escherichia coli Cells. Biochemistry. 2017;56:3725-3732 pubmed publisher..Among the four dxNs, only dxA directed a moderate frequency of dCMP misincorporation. These results provided important insights into the impact of the C3'-epimeric lesions on DNA replication in E. coli cells...
- Brown J, Haas E, James B, Hunt D, Liu J, Pace N. Phylogenetic analysis and evolution of RNase P RNA in proteobacteria. J Bacteriol. 1991;173:3855-63 pubmed..Phylogenetic comparisons indicate that these sequences are not homologous and that any similarity in the structures is, at best, tenuous. ..
- Huang J, Cao C, Lutkenhaus J. Interaction between FtsZ and inhibitors of cell division. J Bacteriol. 1996;178:5080-5 pubmed..However, interactions were observed among various members of the Min system, and MinE was found to reduce the interaction between MinC and MinD. The implications of these findings for cell division are discussed. ..
- Gu W, Williams D, Aldrich H, Xie G, Gabriel D, Jensen R. The aroQ and pheA domains of the bifunctional P-protein from Xanthomonas campestris in a context of genomic comparison. Microb Comp Genomics. 1997;2:141-58 pubmed..PheA family of proteins is presented. A serC gene located immediately upstream of X. campestris aroQp.pheA appears to reflect a conserved gene organization, and both may belong to a single transcriptional unit. ..
- Wang Z. Translesion synthesis by the UmuC family of DNA polymerases. Mutat Res. 2001;486:59-70 pubmed..Recent studies of the UmuC superfamily are summarized and evidence is presented suggesting that this family of DNA polymerases is involved in translesion DNA synthesis. ..
- Prabhu V, Simons A, Iwasaki H, Gai D, Simmons D, Chen J. p53 blocks RuvAB promoted branch migration and modulates resolution of Holliday junctions by RuvC. J Mol Biol. 2002;316:1023-32 pubmed..These results suggest that p53 could have similar effects on eukaryotic homologues of RuvABC and thus have a direct role in recombinational DNA repair. ..
- Wise E, Yew W, Babbitt P, Gerlt J, Rayment I. Homologous (beta/alpha)8-barrel enzymes that catalyze unrelated reactions: orotidine 5'-monophosphate decarboxylase and 3-keto-L-gulonate 6-phosphate decarboxylase. Biochemistry. 2002;41:3861-9 pubmed..Accordingly, sequence or structure homology alone cannot be used to infer the functions of new proteins discovered in genome projects. ..
- DeLisa M, Samuelson P, Palmer T, Georgiou G. Genetic analysis of the twin arginine translocator secretion pathway in bacteria. J Biol Chem. 2002;277:29825-31 pubmed..This result indicates that twin arginine translocation does not require the presence of an arginine dipeptide within the conserved sequence motif. ..
- Chen M, Xie K, Jiang F, Yi L, Dalbey R. YidC, a newly defined evolutionarily conserved protein, mediates membrane protein assembly in bacteria. Biol Chem. 2002;383:1565-72 pubmed..The role of YidC in the membrane insertion pathway will be reviewed. ..
- Mansy S, Wu S, Cowan J. Iron-sulfur cluster biosynthesis: biochemical characterization of the conformational dynamics of Thermotoga maritima IscU and the relevance for cellular cluster assembly. J Biol Chem. 2004;279:10469-75 pubmed..To describe such proteins, we introduce the term multiple discrete conformers...
- Liu G, Li Z, Chiang Y, Acton T, Montelione G, Murray D, et al. High-quality homology models derived from NMR and X-ray structures of E. coli proteins YgdK and Suf E suggest that all members of the YgdK/Suf E protein family are enhancers of cysteine desulfurases. Protein Sci. 2005;14:1597-608 pubmed..The present study exemplifies that "refined" selection of two (or more) targets enables high-quality homology modeling of large protein families. ..
- Yi L, Dalbey R. Oxa1/Alb3/YidC system for insertion of membrane proteins in mitochondria, chloroplasts and bacteria (review). Mol Membr Biol. 2005;22:101-11 pubmed..Additional roles of the Oxa1/Alb3/YidC members may be in the lateral integration of proteins into the lipid bilayer, and in the folding and assembly of proteins into membrane protein complexes. ..
- Lee I, Berdis A, Suzuki C. Recent developments in the mechanistic enzymology of the ATP-dependent Lon protease from Escherichia coli: highlights from kinetic studies. Mol Biosyst. 2006;2:477-83 pubmed
- Kiser P, Lodowski D, Palczewski K. Purification, crystallization and structure determination of native GroEL from Escherichia coli lacking bound potassium ions. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007;63:457-61 pubmed..In general, the structure is similar to the previously determined wild-type GroEL crystal structure with some differences in regard to temperature-factor distribution. ..
- Vitko J, Rujan I, Androga L, Mukerji I, Bolton P. Molecular beacon-equilibrium cyclization detection of DNA-protein complexes. Biophys J. 2007;93:3210-7 pubmed..These MBEC results have been compared with those from other approaches. ..
- Chung D, Min Z, Wang B, Kushner S. Single amino acid changes in the predicted RNase H domain of Escherichia coli RNase G lead to complementation of RNase E deletion mutants. RNA. 2010;16:1371-85 pubmed publisher..Analysis of rneDelta1018/rng-219 and rneDelta1018/rng-248 double mutants has provided interesting insights into the distinct roles of RNase E and RNase G in mRNA decay and tRNA processing. ..
- Cavanagh A, Chandrangsu P, Wassarman K. 6S RNA regulation of relA alters ppGpp levels in early stationary phase. Microbiology. 2010;156:3791-800 pubmed publisher..We suggest a role for 6S RNA in modulating transcription of several global regulators directly, including relA, to downregulate expression of key pathways in response to changing environmental conditions. ..
- Gruenig M, Stohl E, Chitteni Pattu S, Seifert H, Cox M. Less is more: Neisseria gonorrhoeae RecX protein stimulates recombination by inhibiting RecA. J Biol Chem. 2010;285:37188-97 pubmed publisher..Thus, in N. gonorrhoeae, recombinational processes are facilitated by RecX(Ng) protein-mediated limitations on RecA(Ng) filament presence and/or length to achieve maximal function...
- Gruenig M, Lu D, Won S, Dulberger C, Manlick A, Keck J, et al. Creating directed double-strand breaks with the Ref protein: a novel RecA-dependent nuclease from bacteriophage P1. J Biol Chem. 2011;286:8240-51 pubmed publisher..We propose that enhancement of recombination by Ref reflects the introduction of directed, recombinogenic double-strand breaks...
- Mohr S, Ghanem E, Smith W, Sheeter D, Qin Y, King O, et al. Thermostable group II intron reverse transcriptase fusion proteins and their use in cDNA synthesis and next-generation RNA sequencing. RNA. 2013;19:958-70 pubmed publisher..Our findings demonstrate novel biochemical activities and inherent advantages of group II intron RTs for research, biotechnological, and diagnostic methods, with potentially wide applications. ..
- Douville K, Leonard M, Brundage L, Nishiyama K, Tokuda H, Mizushima S, et al. Band 1 subunit of Escherichia coli preportein translocase and integral membrane export factor P12 are the same protein. J Biol Chem. 1994;269:18705-7 pubmed..This finding completes the identification of the polypeptides that are essential for catalysis of preprotein translocation. ..
- Schumacher M, MacDonald J, Bjorkman J, Mowbray S, Brennan R. Structural analysis of the purine repressor, an Escherichia coli DNA-binding protein. J Biol Chem. 1993;268:12282-8 pubmed
- Lee M, Nittayajarn A, Ross R, Rothschild C, Parsonage D, Claiborne A, et al. Characterization of Enterococcus faecalis alkaline phosphatase and use in identifying Streptococcus agalactiae secreted proteins. J Bacteriol. 1999;181:5790-9 pubmed..AP acts as a reporter enzyme in GBS, GAS, and E. faecalis and is expected to be useful in a variety of gram-positive bacteria...
- Nichols T, Whitehouse C, Austin F. Transcriptional analysis of a superoxide dismutase gene of Borrelia burgdorferi. FEMS Microbiol Lett. 2000;183:37-42 pubmed..burgdorferi. A transcriptional start site of this operon, containing -10 and -35 regions of a sigma(70)-type promoter, was mapped to 100 bp upstream of the ATG start codon of secA. ..
- Lusetti S, Shaw J, Cox M. Magnesium ion-dependent activation of the RecA protein involves the C terminus. J Biol Chem. 2003;278:16381-8 pubmed..We propose that the C terminus acts as a regulatory switch, modulating the access of double-stranded DNA to the presynaptic filament and thereby inhibiting homologous DNA pairing and strand exchange at low magnesium ion concentrations. ..
- Dubey A, Baker C, Suzuki K, Jones A, Pandit P, Romeo T, et al. CsrA regulates translation of the Escherichia coli carbon starvation gene, cstA, by blocking ribosome access to the cstA transcript. J Bacteriol. 2003;185:4450-60 pubmed..These results establish that CsrA regulates translation of cstA by sterically interfering with ribosome binding. ..
- Harrison C. GrpE, a nucleotide exchange factor for DnaK. Cell Stress Chaperones. 2003;8:218-24 pubmed..This review describes the molecular biology of GrpE and focuses on the structural and kinetic aspects of nucleotide exchange, peptide release, and the thermosensor hypothesis. ..
- Brockstedt D, Bahjat K, Giedlin M, Liu W, Leong M, Luckett W, et al. Killed but metabolically active microbes: a new vaccine paradigm for eliciting effector T-cell responses and protective immunity. Nat Med. 2005;11:853-60 pubmed..Microbial KBMA vaccines used either as a recombinant vaccine platform or as a modified form of the pathogen itself may have broad use for the treatment of infectious disease and cancer. ..
- Tao H, Hasona A, Do P, Ingram L, Shanmugam K. Global gene expression analysis revealed an unsuspected deo operon under the control of molybdate sensor, ModE protein, in Escherichia coli. Arch Microbiol. 2005;184:225-33 pubmed..This study identified additional targets, such as deo and opp, for the Mo-dependent control in E. coli. ..
- Kim J, White K, Winfree E. Construction of an in vitro bistable circuit from synthetic transcriptional switches. Mol Syst Biol. 2006;2:68 pubmed..Construction of larger synthetic circuits provides a unique opportunity for evaluating model inference, prediction, and design of complex biochemical systems and could be used to control nanoscale devices and artificial cells. ..
- Yuan J, Phillips G, Dalbey R. Isolation of cold-sensitive yidC mutants provides insights into the substrate profile of the YidC insertase and the importance of transmembrane 3 in YidC function. J Bacteriol. 2007;189:8961-72 pubmed..One pair of mutations suggests an interaction between TM2 and TM3 of YidC. The studies reveal the critical regions of the YidC protein and provide insight into the substrate profile of the YidC insertase. ..
- Reimann S, Wolfe A. A critical process controlled by MalT and OmpR is revealed through synthetic lethality. J Bacteriol. 2009;191:5320-4 pubmed publisher..Here we report that cells lacking OmpR and harboring constitutively active MalT undergo premature death that involves increased expression of the outer membrane porin LamB...
- Lu D, Windsor M, Gellman S, Keck J. Peptide inhibitors identify roles for SSB C-terminal residues in SSB/exonuclease I complex formation. Biochemistry. 2009;48:6764-71 pubmed publisher..Together, these data identify roles for residues in the SSB-Ct that are important for SSB complex formation with its protein partners. ..
- Berenson C, Nawar H, Yohe H, Castle S, Ashline D, Reinhold V, et al. Mammalian cell ganglioside-binding specificities of E. coli enterotoxins LT-IIb and variant LT-IIb(T13I). Glycobiology. 2010;20:41-54 pubmed publisher..These studies underscore the high degree of specificity required for ganglioside-protein interactions. ..
- Peters J, Becker N, Rueter E, Bajzer Z, Kahn J, Maher L. Quantitative methods for measuring DNA flexibility in vitro and in vivo. Methods Enzymol. 2011;488:287-335 pubmed publisher..coli HU protein and eukaryotic HMGB proteins. ..
- Thao S, Chen C, Zhu H, Escalante Semerena J. N?-lysine acetylation of a bacterial transcription factor inhibits Its DNA-binding activity. PLoS ONE. 2010;5:e15123 pubmed publisher..These findings further the likelihood that reversible N(?)-Lys acetylation of transcription factors is a mode of regulation of gene expression used by all cells. ..
- Lu D, Myers A, George N, Keck J. Mechanism of Exonuclease I stimulation by the single-stranded DNA-binding protein. Nucleic Acids Res. 2011;39:6536-45 pubmed publisher..Taken together, these studies delineate stimulatory roles for SSB in which protein interactions and ssDNA binding are both important for maximal activity of its protein partners. ..
- Freddolino P, Amini S, Tavazoie S. Newly identified genetic variations in common Escherichia coli MG1655 stock cultures. J Bacteriol. 2012;194:303-6 pubmed publisher..The mutations are likely to cause loss of function of the glpR and crl genes, which may have serious implications for physiological experiments using the affected strains...
- Raterman E, Shapiro D, Stevens D, Schwartz K, Welch R. Genetic analysis of the role of yfiR in the ability of Escherichia coli CFT073 to control cellular cyclic dimeric GMP levels and to persist in the urinary tract. Infect Immun. 2013;81:3089-98 pubmed publisher..coli in the urinary tract and implicate the c-di-GMP regulatory system as an important factor in the persistence of uropathogenic E. coli in vivo. ..
- Shi A, Zheng H, Yomano L, York S, Shanmugam K, Ingram L. Plasmidic Expression of nemA and yafC* Increased Resistance of Ethanologenic Escherichia coli LY180 to Nonvolatile Side Products from Dilute Acid Treatment of Sugarcane Bagasse and Artificial Hydrolysate. Appl Environ Microbiol. 2016;82:2137-2145 pubmed publisher..Expression of the nemA or yafC* plasmid in LY180 doubled the rate of ethanol production from the vacuum-treated sugarcane bagasse hydrolysate. ..
- Ranjan N, Story S, Fulcrand G, Leng F, Ahmad M, King A, et al. Selective Inhibition of Escherichia coli RNA and DNA Topoisomerase I by Hoechst 33258 Derived Mono- and Bisbenzimidazoles. J Med Chem. 2017;60:4904-4922 pubmed publisher..2-23.4 °C), and cytotoxicity studies show similar variation dependent upon the side chain length. Modeling studies suggest critical interactions between the inhibitor side chain and amino acids of the active site of DNA topoisomerase I. ..
- MacGregor C, Arora S, Hager P, Dail M, Phibbs P. The nucleotide sequence of the Pseudomonas aeruginosa pyrE-crc-rph region and the purification of the crc gene product. J Bacteriol. 1996;178:5627-35 pubmed..Antibody to the purified Crc protein reacted with proteins of similar size in crude extracts from Pseudomonas putida and Pseudomonas fluorescens, suggesting a common mechanism of catabolite repression in these three species. ..
- Tsolis R, Bäumler A, Heffron F, Stojiljkovic I. Contribution of TonB- and Feo-mediated iron uptake to growth of Salmonella typhimurium in the mouse. Infect Immun. 1996;64:4549-56 pubmed..The tonB feoB double mutant, given intraperitoneally, was able to infect the liver and spleen at wild-type doses, indicating that additional iron acquisition systems are used during growth at systemic sites of infection. ..
- Woodbury R, Topping T, Diamond D, Suciu D, Kumamoto C, Hardy S, et al. Complexes between protein export chaperone SecB and SecA. Evidence for separate sites on SecA providing binding energy and regulatory interactions. J Biol Chem. 2000;275:24191-8 pubmed..The region of SecA involved in activation of the complexes was defined as the extreme carboxyl-terminal 21 aminoacyl residues. ..
- Yahr T, Wickner W. Evaluating the oligomeric state of SecYEG in preprotein translocase. EMBO J. 2000;19:4393-401 pubmed..These studies suggest that the active form of preprotein translocase is monomeric SecYEG. ..
- Barnett M, Zolkiewski M. Site-directed mutagenesis of conserved charged amino acid residues in ClpB from Escherichia coli. Biochemistry. 2002;41:11277-83 pubmed..Interestingly, we have identified a conserved residue within the C-terminal domain (Arg819) which does not participate directly in nucleotide binding but is essential for the chaperone activity of ClpB...
- McCool J, Ford C, Sandler S. A dnaT mutant with phenotypes similar to those of a priA2::kan mutant in Escherichia coli K-12. Genetics. 2004;167:569-78 pubmed publisher..coli K-12. Instead, this mutant dnaT gene was found to complement the E. coli K-12 dnaT822 mutant phenotypes. The significance of these results is discussed in terms of models for replication restart...
- Drees J, Lusetti S, Cox M. Inhibition of RecA protein by the Escherichia coli RecX protein: modulation by the RecA C terminus and filament functional state. J Biol Chem. 2004;279:52991-7 pubmed..We further identified a C-terminal point mutation in RecA protein (E343K) that significantly alters the interaction between RecA and RecX proteins. ..
- Gralnick J, Brown C, Newman D. Anaerobic regulation by an atypical Arc system in Shewanella oneidensis. Mol Microbiol. 2005;56:1347-57 pubmed..coli, HptA. This protein alone is unable to compensate for the lack of arcB in E. coli, indicating that another protein is required in addition to HptA to activate ArcA in S. oneidensis. ..
- Vrentas C, Gaal T, Ross W, Ebright R, Gourse R. Response of RNA polymerase to ppGpp: requirement for the omega subunit and relief of this requirement by DksA. Genes Dev. 2005;19:2378-87 pubmed..Furthermore, these results suggest that either one or both of two short segments in the beta' subunit that physically link omega to the ppGpp-binding region of the enzyme may play crucial roles in ppGpp and DksA function. ..
- Shih Y, Kawagishi I, Rothfield L. The MreB and Min cytoskeletal-like systems play independent roles in prokaryotic polar differentiation. Mol Microbiol. 2005;58:917-28 pubmed..Thus, the two bacterial cytoskeletal-like systems act independently on different aspects of cell polarization. ..
- Borths E, Poolman B, Hvorup R, Locher K, Rees D. In vitro functional characterization of BtuCD-F, the Escherichia coli ABC transporter for vitamin B12 uptake. Biochemistry. 2005;44:16301-9 pubmed
- Fiala K, Hypes C, Suo Z. Mechanism of abasic lesion bypass catalyzed by a Y-family DNA polymerase. J Biol Chem. 2007;282:8188-98 pubmed..Our results suggest that abasic lesion bypass requires tight regulation to maintain genomic stability...
- Cummings W, Yabuki M, Ordinario E, Bednarski D, Quay S, Maizels N. Chromatin structure regulates gene conversion. PLoS Biol. 2007;5:e246 pubmed..Thus, chromatin structure regulates homology-directed repair. These results suggest that histone modifications may contribute to maintaining genomic stability by preventing recombination between repetitive sequences. ..
- Bendezú F, de Boer P. Conditional lethality, division defects, membrane involution, and endocytosis in mre and mrd shape mutants of Escherichia coli. J Bacteriol. 2008;190:1792-811 pubmed..Both FtsZ and MinD readily assembled on intracytoplasmic membrane surfaces, and we propose that this contributes significantly to the lethal division block seen in all shape mutants under nonpermissive conditions. ..
- Yan A, Kiley P. Dissecting the role of the N-terminal region of the Escherichia coli global transcription factor FNR. J Bacteriol. 2008;190:8230-3 pubmed publisher..We found that truncation mutants that removed residues 2 to 16 and 2 to 17 had wild-type levels of FNR protein but surprisingly altered O(2) regulation. ..
- Thoden J, Holden H, Firestine S. Structural analysis of the active site geometry of N5-carboxyaminoimidazole ribonucleotide synthetase from Escherichia coli. Biochemistry. 2008;47:13346-53 pubmed publisher..Using the information derived from the two structures reported here, coupled with molecular modeling, we propose a catalytic mechanism for N(5)-CAIR synthetase. ..
- Fowler Z, Gikandi W, Koffas M. Increased malonyl coenzyme A biosynthesis by tuning the Escherichia coli metabolic network and its application to flavanone production. Appl Environ Microbiol. 2009;75:5831-9 pubmed publisher..As a result, the specific flavanone production from our optimally engineered strains was increased by over 660% for naringenin (15 to 100 mg/liter/optical density unit [OD]) and by over 420% for eriodictyol (13 to 55 mg/liter/OD). ..
- Tomanicek S, Blakeley M, Cooper J, Chen Y, Afonine P, Coates L. Neutron diffraction studies of a class A beta-lactamase Toho-1 E166A/R274N/R276N triple mutant. J Mol Biol. 2010;396:1070-80 pubmed publisher..Thus, this neutron structure is in agreement with a proposed mechanism for acylation that identifies Glu166 as the general base for catalysis. ..
- Nawar H, Berenson C, Hajishengallis G, Takematsu H, Mandell L, Clare R, et al. Binding to gangliosides containing N-acetylneuraminic acid is sufficient to mediate the immunomodulatory properties of the nontoxic mucosal adjuvant LT-IIb(T13I). Clin Vaccine Immunol. 2010;17:969-78 pubmed publisher..The nontoxic mutant enterotoxin LT-IIb(T13I), therefore, is potentially a new and safe human mucosal adjuvant. ..
- Horsman G, Chen Y, Thorson J, Shen B. Polyketide synthase chemistry does not direct biosynthetic divergence between 9- and 10-membered enediynes. Proc Natl Acad Sci U S A. 2010;107:11331-5 pubmed publisher..These findings establish a starting point for determining the origins of this biosynthetic divergence. ..
- Ha K, Toulokhonov I, Vassylyev D, Landick R. The NusA N-terminal domain is necessary and sufficient for enhancement of transcriptional pausing via interaction with the RNA exit channel of RNA polymerase. J Mol Biol. 2010;401:708-25 pubmed publisher..Finally, we report evidence that the NusA NTD recognizes duplex RNA in the RNA exit channel. ..
- Koh J, Shkel I, Saecker R, Record M. Nonspecific DNA binding and bending by HU??: interfaces of the three binding modes characterized by salt-dependent thermodynamics. J Mol Biol. 2011;410:241-67 pubmed publisher..Structural models consistent with the observed Sk(i) values are proposed. ..
- Jobling M, Holmes R. Type II heat-labile enterotoxins from 50 diverse Escherichia coli isolates belong almost exclusively to the LT-IIc family and may be prophage encoded. PLoS ONE. 2012;7:e29898 pubmed publisher..coli. ..
- Myers K, Yan H, Ong I, Chung D, Liang K, Tran F, et al. Genome-scale analysis of escherichia coli FNR reveals complex features of transcription factor binding. PLoS Genet. 2013;9:e1003565 pubmed publisher..Such combinatorial regulation may allow Escherichia coli to respond rapidly to environmental changes and confer an ecological advantage in the anaerobic but nutrient-fluctuating environment of the mammalian gut. ..
- Meier E, Daitch A, Yao Q, Bhargava A, Jensen G, Goley E. FtsEX-mediated regulation of the final stages of cell division reveals morphogenetic plasticity in Caulobacter crescentus. PLoS Genet. 2017;13:e1006999 pubmed publisher..crescentus stalks. Our data uncover unanticipated morphogenetic plasticity in C. crescentus, with loss of FtsE causing a stalk-like program to take over at failed division sites...
- Andres D, Shao H, Crick D, Finlin B. Expression cloning of a novel farnesylated protein, RDJ2, encoding a DnaJ protein homologue. Arch Biochem Biophys. 1997;346:113-24 pubmed
- Hasona A, Self W, Ray R, Shanmugam K. Molybdate-dependent transcription of hyc and nar operons of Escherichia coli requires MoeA protein and ModE-molybdate. FEMS Microbiol Lett. 1998;169:111-6 pubmed..It is proposed that the MoeA-catalyzed product, an activated form of molybdate, interacts with a transcriptional activator/regulator other than ModE and regulates hyc and nar operons. ..
- Kiley P, Beinert H. Oxygen sensing by the global regulator, FNR: the role of the iron-sulfur cluster. FEMS Microbiol Rev. 1998;22:341-52 pubmed..Attempts to reduce the [4Fe-4S]2+ cluster of FNR with dithionite indicated that the redox potential of the +1/+2 couple is < or = -650 mV and that the [4Fe-4S]+ cluster form is, therefore, not likely to occur in vivo. ..
- Post G, Swiderski C, Waldrop B, Salty L, Glembotski C, Wolthuis R, et al. Guanine nucleotide exchange factor-like factor (Rlf) induces gene expression and potentiates alpha 1-adrenergic receptor-induced transcriptional responses in neonatal rat ventricular myocytes. J Biol Chem. 2002;277:15286-92 pubmed..Overall, this study supports the concept that Rlf and Ral are important previously unrecognized signaling components that regulate transcriptional responses in myocardial cells. ..