Experts and Doctors on escherichia coli proteins in Switzerland


Locale: Switzerland
Topic: escherichia coli proteins

Top Publications

  1. Bothmann H, Pluckthun A. Selection for a periplasmic factor improving phage display and functional periplasmic expression. Nat Biotechnol. 1998;16:376-80 pubmed
    ..This occurs as a result of an increase in the amount of hybrid protein displayed on the phage. Coexpression of skp also increases the functional yield of scFv fragments when expressed by secretion to the periplasm. ..
  2. Oster s M, Stotz A, Schmid Nuoffer S, Jenal U. Identification and transcriptional control of the genes encoding the Caulobacter crescentus ClpXP protease. J Bacteriol. 1999;181:3039-50 pubmed
    ..crescentus. Determination of the numbers of ClpP and ClpX molecules per cell suggested that ClpX is the limiting component compared with ClpP...
  3. Landini P, Volkert M. Regulatory responses of the adaptive response to alkylation damage: a simple regulon with complex regulatory features. J Bacteriol. 2000;182:6543-9 pubmed
  4. Panne D, Muller S, Wirtz S, Engel A, Bickle T. The McrBC restriction endonuclease assembles into a ring structure in the presence of G nucleotides. EMBO J. 2001;20:3210-7 pubmed
    ..In the presence of McrC, a subunit that is essential for DNA cleavage, the tetradecameric species was the major form of the endonuclease. ..
  5. Stahlberg H, Engel A, Philippsen A. Assessing the structure of membrane proteins: combining different methods gives the full picture. Biochem Cell Biol. 2002;80:563-8 pubmed
    ..This illustrates that the combination of different methods in structural biology reveals more than each method alone. ..
  6. Genevaux P, Schwager F, Georgopoulos C, Kelley W. Scanning mutagenesis identifies amino acid residues essential for the in vivo activity of the Escherichia coli DnaJ (Hsp40) J-domain. Genetics. 2002;162:1045-53 pubmed
    ..We propose that these clustered residues impart critical sequence determinants necessary for J-domain catalytic activity and reversible contact interface with the DnaK ATPase domain. ..
  7. Enggist E, Schneider M, Schulz H, Thöny Meyer L. Biochemical and mutational characterization of the heme chaperone CcmE reveals a heme binding site. J Bacteriol. 2003;185:175-83 pubmed
    ..Isolation and characterization of the heme-binding peptides obtained after a tryptic digest of wild-type and H130C CcmE support the hypothesis that heme is bound covalently at a vinyl group. ..
  8. Sauer U, Canonaco F, Heri S, Perrenoud A, Fischer E. The soluble and membrane-bound transhydrogenases UdhA and PntAB have divergent functions in NADPH metabolism of Escherichia coli. J Biol Chem. 2004;279:6613-9 pubmed
    ..metabolism with an extraordinary flexibility to cope with varying catabolic and anabolic demands, which raises two general questions: why do only a few bacteria contain both isoforms, and how do other organisms manage NADPH metabolism? ..
  9. Capitani G, Eidam O, Grutter M. Evidence for a novel domain of bacterial outer membrane ushers. Proteins. 2006;65:816-23 pubmed
    ..Several mutations reported to abolish in vivo pilus formation cluster in this region, underlining its functional importance. ..

More Information

Publications119 found, 100 shown here

  1. Rozhkova A, Glockshuber R. Kinetics of the intramolecular disulfide exchange between the periplasmic domains of DsbD. J Mol Biol. 2007;367:1162-70 pubmed
    ..The rate of 23 s(-1) for the intramolecular disulfide exchange in the fusions was independent of the linker length and may represent the upper limit for the substrate turnover of full-length DsbD. ..
  2. Ihssen J, Kowarik M, Dilettoso S, Tanner C, Wacker M, Thöny Meyer L. Production of glycoprotein vaccines in Escherichia coli. Microb Cell Fact. 2010;9:61 pubmed publisher
    ..The described methodologies constitute an important step towards cost-effective in vivo production of conjugate vaccines, which in future may be used for combating severe infectious diseases, particularly in developing countries. ..
  3. Pusnik M, Schmidt O, Perry A, Oeljeklaus S, Niemann M, Warscheid B, et al. Mitochondrial preprotein translocase of trypanosomatids has a bacterial origin. Curr Biol. 2011;21:1738-43 pubmed publisher
    ..This suggests that the protein import channel in trypanosomes is a relic of an archaic protein transport system that was operational in the ancestor of all eukaryotes. ..
  4. Burmann B, Hiller S. Solution NMR studies of membrane-protein-chaperone complexes. Chimia (Aarau). 2012;66:759-63 pubmed publisher
    ..Thirdly, we use amino acid mutation analysis to show that the interaction of OmpX to Skp is not dominated by the two most hydrophobic segments of OmpX. ..
  5. Vögeli B, Pervushin K. TROSY experiment for refinement of backbone psi and phi by simultaneous measurements of cross-correlated relaxation rates and 3,4J(H alpha HN) coupling constants. J Biomol NMR. 2002;24:291-300 pubmed
    ..coli Heme Chaperon protein CcmE is described. Overall good agreement is achieved between psi and phi angles measured with the new experiment and the average values determined from an ensemble of 20 NMR conformers. ..
  6. Cranz Mileva S, Imkamp F, Kolygo K, Maglica Z, Kress W, Weber Ban E. The flexible attachment of the N-domains to the ClpA ring body allows their use on demand. J Mol Biol. 2008;378:412-24 pubmed publisher
    ..These results suggest that the flexible attachment of the N-domains to ClpA allows their recruitment to the pore on demand for certain substrates, while allowing them to move out of the way for substrates binding directly to the pore. ..
  7. Weidenhaupt M, Rossi P, Beck C, Fischer H, Hennecke H. Bradyrhizobium japonicum possesses two discrete sets of electron transfer flavoprotein genes: fixA, fixB and etfS, etfL. Arch Microbiol. 1996;165:169-78 pubmed
    ..japonicum are members of that group. B. japonicum is the first example of an organism in which genes for proteins of both groups I and II of the ETF family have been identified...
  8. Koebnik R, Locher K, Van Gelder P. Structure and function of bacterial outer membrane proteins: barrels in a nutshell. Mol Microbiol. 2000;37:239-53 pubmed
    ..Our review is aimed at discussing their common principles and peculiarities as well as open questions associated with them. ..
  9. Pervushin K, Vögeli B. Observation of individual transitions in magnetically equivalent spin systems. J Am Chem Soc. 2003;125:9566-7 pubmed
    ..The use of the experiments for the measurement of RDCs is demonstrated with two proteins, one weakly aligned by means of Pf1 phages and the other using a naturally present paramagnetic heme group...
  10. Ben Zvi A, De Los Rios P, Dietler G, Goloubinoff P. Active solubilization and refolding of stable protein aggregates by cooperative unfolding action of individual hsp70 chaperones. J Biol Chem. 2004;279:37298-303 pubmed
  11. Lee D, Hilty C, Wider G, Wuthrich K. Effective rotational correlation times of proteins from NMR relaxation interference. J Magn Reson. 2006;178:72-6 pubmed
  12. Rozhkova A, Glockshuber R. Thermodynamic aspects of DsbD-mediated electron transport. J Mol Biol. 2008;380:783-8 pubmed publisher
    ..246 V. The results show that all steps in the DsbD-mediated electron flow are thermodynamically favorable. ..
  13. Z hringer F, Lacanna E, Jenal U, Schirmer T, Boehm A. Structure and signaling mechanism of a zinc-sensory diguanylate cyclase. Structure. 2013;21:1149-57 pubmed publisher
    ..The study outlines the structural principles of this zinc sensor. Zinc binding seems to regulate the activity of the catalytic GGDEF domains by impeding their mobility and thus preventing productive encounter of the two GTP substrates...
  14. van der Ploeg J, Iwanicka Nowicka R, Bykowski T, Hryniewicz M, Leisinger T. The Escherichia coli ssuEADCB gene cluster is required for the utilization of sulfur from aliphatic sulfonates and is regulated by the transcriptional activator Cbl. J Biol Chem. 1999;274:29358-65 pubmed
    ..Integration host factor could also occupy three binding sites in the ssu promoter region but had no influence on expression of a chromosomal ssuE'-lacZ fusion. ..
  15. Branny P, Pearson J, Pesci E, Kohler T, Iglewski B, Van Delden C. Inhibition of quorum sensing by a Pseudomonas aeruginosa dksA homologue. J Bacteriol. 2001;183:1531-9 pubmed
    ..Our results suggest that the overproduction of the P. aeruginosa DksA homologue inhibits quorum-sensing-dependent virulence factor production by downregulating the transcription of the autoinducer synthase gene rhlI. ..
  16. Fernandez C, Adeishvili K, Wuthrich K. Transverse relaxation-optimized NMR spectroscopy with the outer membrane protein OmpX in dihexanoyl phosphatidylcholine micelles. Proc Natl Acad Sci U S A. 2001;98:2358-63 pubmed
  17. Locher K, Borths E. ABC transporter architecture and mechanism: implications from the crystal structures of BtuCD and BtuF. FEBS Lett. 2004;564:264-8 pubmed
    ..In the following, we will briefly review the results of these structural investigations and outline their mechanistic implications...
  18. Gentschev I, Spreng S, Sieber H, Ures J, Mollet F, Collioud A, et al. Vivotif--a 'magic shield' for protection against typhoid fever and delivery of heterologous antigens. Chemotherapy. 2007;53:177-80 pubmed
    ..Recently, we successfully used Ty21a for antigen delivery via the haemolysin secretion system of Escherichia coli, which allows efficient protein secretion from the carrier bacteria. ..
  19. Bost S, Belin D. A new genetic selection identifies essential residues in SecG, a component of the Escherichia coli protein export machinery. EMBO J. 1995;14:4412-21 pubmed
    ..Most suppressors map to secY, and several are allele-specific. Finally, SecG overexpression accelerates the kinetics of protein export, suggesting that there are two types of functional translocation complexes: with or without SecG...
  20. Scott H, Chen H, Rossier C, Lalioti M, Antonarakis S. Isolation of a human gene (HES1) with homology to an Escherichia coli and a zebrafish protein that maps to chromosome 21q22.3. Hum Genet. 1997;99:616-23 pubmed
    ..In addition, the initial method of EST identification for gene isolation presented here is valid for many genes and can be used to obtain initial sequence contigs without cloning or library screening. ..
  21. Eichhorn E, van der Ploeg J, Leisinger T. Characterization of a two-component alkanesulfonate monooxygenase from Escherichia coli. J Biol Chem. 1999;274:26639-46 pubmed
    ..SsuE is the FMN reducing enzyme providing SsuD with FMNH(2). ..
  22. Brombacher E, Dorel C, Zehnder A, Landini P. The curli biosynthesis regulator CsgD co-ordinates the expression of both positive and negative determinants for biofilm formation in Escherichia coli. Microbiology. 2003;149:2847-57 pubmed
    ..coli by contemporary activation of adhesion positive determinants (the curli-encoding csg operons and the product of the yaiC gene) and repression of negative effectors such as yagS and pepD. ..
  23. Horst R, Wider G, Fiaux J, Bertelsen E, Horwich A, Wuthrich K. Proton-proton Overhauser NMR spectroscopy with polypeptide chains in large structures. Proc Natl Acad Sci U S A. 2006;103:15445-50 pubmed
  24. Grimshaw J, Stirnimann C, Brozzo M, Malojcic G, Grutter M, Capitani G, et al. DsbL and DsbI form a specific dithiol oxidase system for periplasmic arylsulfate sulfotransferase in uropathogenic Escherichia coli. J Mol Biol. 2008;380:667-80 pubmed publisher
    ..We propose that the DsbL/DsbI pair of uropathogenic E. coli was acquired as an additional, specific redox couple that guarantees biological activity of ASST. ..
  25. Jeckelmann J, Harder D, Mari S, Meury M, Ucurum Z, Muller D, et al. Structure and function of the glucose PTS transporter from Escherichia coli. J Struct Biol. 2011;176:395-403 pubmed publisher
    ..This work presents the structure of a glucose PTS transporter at the highest resolution achieved so far and sets the basis for future structural studies. ..
  26. Schulz H, Fabianek R, Pellicioli E, Hennecke H, Thony Meyer L. Heme transfer to the heme chaperone CcmE during cytochrome c maturation requires the CcmC protein, which may function independently of the ABC-transporter CcmAB. Proc Natl Acad Sci U S A. 1999;96:6462-7 pubmed
    ..CcmD was found to be involved in stabilizing the heme chaperone CcmE in the membrane. We propose a heme-trafficking pathway as part of a substantially revised model for cytochrome c maturation in E. coli...
  27. Mason C, Dunner J, Indra P, Colangelo T. Heat-induced expression and chemically induced expression of the Escherichia coli stress protein HtpG are affected by the growth environment. Appl Environ Microbiol. 1999;65:3433-40 pubmed
    ..The results demonstrate the importance of considering differences in growth environment in order to better understand the nature of the response to an imposed stress condition. ..
  28. Dartigalongue C, Loferer H, Raina S. EcfE, a new essential inner membrane protease: its role in the regulation of heat shock response in Escherichia coli. EMBO J. 2001;20:5908-18 pubmed
    ..Temperature-sensitive mutants of this gene were isolated mapping to the catalytic site and other domains that exhibited constitutively elevated levels of both heat shock regulons. ..
  29. Ren Q, Ahuja U, Thöny Meyer L. A bacterial cytochrome c heme lyase. CcmF forms a complex with the heme chaperone CcmE and CcmH but not with apocytochrome c. J Biol Chem. 2002;277:7657-63 pubmed
    ..We propose that CcmFH forms a bacterial heme lyase complex for the transfer of heme from CcmE to apocytochrome c. ..
  30. Boehringer D, Ban N. Trapping the ribosome to control gene expression. Cell. 2007;130:983-5 pubmed
    ..These results have implications for our understanding of the mechanism of translation initiation in general. ..
  31. Abicht H, Martinez J, Layer G, Jahn D, Solioz M. Lactococcus lactis HemW (HemN) is a haem-binding protein with a putative role in haem trafficking. Biochem J. 2012;442:335-43 pubmed publisher
    ..On the basis of these findings, we propose a role of HemW in haem trafficking. HemW-like proteins form a distinct phylogenetic clade that has not previously been recognized...
  32. Korkhov V, Mireku S, Locher K. Structure of AMP-PNP-bound vitamin B12 transporter BtuCD-F. Nature. 2012;490:367-72 pubmed publisher
    ..In combination with engineered disulphide crosslinking and functional assays, our data suggest an unexpected peristaltic transport mechanism that is distinct from those observed in other ABC transporters. ..
  33. Bost S, Silva F, Rudaz C, Belin D. Both transmembrane domains of SecG contribute to signal sequence recognition by the Escherichia coli protein export machinery. Mol Microbiol. 2000;38:575-87 pubmed
    ..Thus, SecG participates in signal sequence recognition, and both transmembrane domains of SecG contribute to ensure normal signal sequence recognition by the translocase. ..
  34. Ahuja U, Thöny Meyer L. Dynamic features of a heme delivery system for cytochrome C maturation. J Biol Chem. 2003;278:52061-70 pubmed
    ..This contradicts the idea of CcmCEF supercomplex formation. Our results favor a model that predicts CcmE to shuttle between CcmC and CcmF for heme delivery. ..
  35. Garcia P, Bradley G, Hayes C, Krintel S, Soultanas P, Janscak P. RPA alleviates the inhibitory effect of vinylphosphonate internucleotide linkages on DNA unwinding by BLM and WRN helicases. Nucleic Acids Res. 2004;32:3771-8 pubmed
  36. Bächler C, Flükiger Brühwiler K, Schneider P, Bähler P, Erni B. From ATP as substrate to ADP as coenzyme: functional evolution of the nucleotide binding subunit of dihydroxyacetone kinases. J Biol Chem. 2005;280:18321-5 pubmed
    ..This conversion of a substrate binding site into a cofactor binding site reflects a remarkable instance of parsimonious evolution. ..
  37. Basle A, Rummel G, Storici P, Rosenbusch J, Schirmer T. Crystal structure of osmoporin OmpC from E. coli at 2.0 A. J Mol Biol. 2006;362:933-42 pubmed
    ..The OmpC structure suggests that not pore size, but electrostatic pore potential and particular atomic details of the pore linings are the critical parameters that physiologically distinguish OmpC from OmpF. ..
  38. Maglica Z, Striebel F, Weber Ban E. An intrinsic degradation tag on the ClpA C-terminus regulates the balance of ClpAP complexes with different substrate specificity. J Mol Biol. 2008;384:503-11 pubmed publisher
    ..This simple mechanism allows for dynamic reallocation of free ClpAP versus ClpAPS in response to the presence of ssrA-tagged substrates. ..
  39. Sendi P, Frei R, Maurer T, Trampuz A, Zimmerli W, Graber P. Escherichia coli variants in periprosthetic joint infection: diagnostic challenges with sessile bacteria and sonication. J Clin Microbiol. 2010;48:1720-5 pubmed publisher
    ..Sonication of an explanted prosthesis allows insight into the lifestyle of bacteria in biofilms. Since sonication fluid also reveals dislodged sessile forms, species identification of such variants may be misleading. ..
  40. Panne D, Raleigh E, Bickle T. McrBs, a modulator peptide for McrBC activity. EMBO J. 1998;17:5477-83 pubmed
    ..We suggest that the role of McrBs is to modulate McrBC activity by binding to McrC. ..
  41. Lacour S, Kolb A, Landini P. Nucleotides from -16 to -12 determine specific promoter recognition by bacterial sigmaS-RNA polymerase. J Biol Chem. 2003;278:37160-8 pubmed
    ..We propose a new sequence, TG(N)0-2CCATA(c/a)T, as consensus -10 sequence for promoters exclusively recognized by EsigmaS. ..
  42. Lukashev A, Fuerer C, Chen M, Searle P, Iggo R. Late expression of nitroreductase in an oncolytic adenovirus sensitizes colon cancer cells to the prodrug CB1954. Hum Gene Ther. 2005;16:1473-83 pubmed
  43. Wassmann P, Chan C, Paul R, Beck A, Heerklotz H, Jenal U, et al. Structure of BeF3- -modified response regulator PleD: implications for diguanylate cyclase activation, catalysis, and feedback inhibition. Structure. 2007;15:915-27 pubmed publisher
    ..Here, a second mode is observed that crosslinks the DGC domains within a PleD dimer. Both modes cause noncompetitive product inhibition by domain immobilization...
  44. Engel P, Goepfert A, Stanger F, Harms A, Schmidt A, Schirmer T, et al. Adenylylation control by intra- or intermolecular active-site obstruction in Fic proteins. Nature. 2012;482:107-10 pubmed publisher
    ..Future studies should reveal how intrinsic or extrinsic factors modulate adenylylation activity by weakening the interaction of ?(inh) with the FIC active site...
  45. Andreozzi S, Miskovic L, Hatzimanikatis V. iSCHRUNK--In Silico Approach to Characterization and Reduction of Uncertainty in the Kinetic Models of Genome-scale Metabolic Networks. Metab Eng. 2016;33:158-168 pubmed publisher
    ..The proposed approach also sets up the foundations of a novel type of approaches for efficient, non-asymptotic, uniform sampling of solution spaces. ..
  46. Feldman M, Wacker M, Hernandez M, Hitchen P, Marolda C, Kowarik M, et al. Engineering N-linked protein glycosylation with diverse O antigen lipopolysaccharide structures in Escherichia coli. Proc Natl Acad Sci U S A. 2005;102:3016-21 pubmed
    ..coli or Pseudomonas aeruginosa O antigens. PglB-mediated transfer of polysaccharides might be valuable for in vivo production of O polysaccharides-protein conjugates for use as antibacterial vaccines. ..
  47. Romero J, Ciabattini A, Guillaume P, Frank G, Ruggiero P, Pettini E, et al. Intranasal administration of the synthetic polypeptide from the C-terminus of the circumsporozoite protein of Plasmodium berghei with the modified heat-labile toxin of Escherichia coli (LTK63) induces a complete protection against malaria challenge. Vaccine. 2009;27:1266-71 pubmed publisher
    ..Here, we show that intranasal administration of the two compounds activates the T and B cell immune response locally and systemically. In addition, a total protection of mice is obtained upon a challenge with live sporozoites. ..
  48. Maglica Z, Kolygo K, Weber Ban E. Optimal efficiency of ClpAP and ClpXP chaperone-proteases is achieved by architectural symmetry. Structure. 2009;17:508-16 pubmed publisher
    ..Thus, we propose that conformational transitions in ClpP are concerted and allosteric effects are transferred simultaneously to both associated chaperones, leading to synchronized activation. ..
  49. Enggist E, Thöny Meyer L. The C-terminal flexible domain of the heme chaperone CcmE is important but not essential for its function. J Bacteriol. 2003;185:3821-7 pubmed
    ..Soluble full-length CcmE had low activity in cytochrome c maturation, indicating the importance of the N-terminal membrane anchor for the in vivo function of CcmE. ..
  50. Fischer E, Sauer U. A novel metabolic cycle catalyzes glucose oxidation and anaplerosis in hungry Escherichia coli. J Biol Chem. 2003;278:46446-51 pubmed
  51. Dubaele S, Lourdel C, Chene P. Study of the ATP-binding site of helicase IV from Escherichia coli. Biochem Biophys Res Commun. 2006;341:828-36 pubmed
    ..This suggests that residues located at the adenine-binding pocket, in addition to be involved in ATP-binding, are important for efficient coupling between ATP hydrolysis and DNA unwinding. ..
  52. Letellier L, Locher K, Plançon L, Rosenbusch J. Modeling ligand-gated receptor activity. FhuA-mediated ferrichrome efflux from lipid vesicles triggered by phage T5. J Biol Chem. 1997;272:1448-51 pubmed
    ..The assay is rapid, reliable, and specific, because other bacteriophages, such as Phi80, fail to trigger channel opening of the FhuA receptor. ..
  53. Pos K, Dimroth P, Bott M. The Escherichia coli citrate carrier CitT: a member of a novel eubacterial transporter family related to the 2-oxoglutarate/malate translocator from spinach chloroplasts. J Bacteriol. 1998;180:4160-5 pubmed
    ..It is suggested that the E. coli CitT protein is a member of a novel family of eubacterial transporters involved in the transport of di- and tricarboxylic acids. ..
  54. Narberhaus F, Urech C, Hennecke H. Characterization of the Bradyrhizobium japonicum ftsH gene and its product. J Bacteriol. 1999;181:7394-7 pubmed
    ..The expression of B. japonicum ftsH in an ftsH-negative Escherichia coli strain significantly enhanced the fitness of this mutant and reduced the steady-state level of sigma(32). ..
  55. Stahlberg H, Braun T, de Groot B, Philippsen A, Borgnia M, Agre P, et al. The 6.9-A structure of GlpF: a basis for homology modeling of the glycerol channel from Escherichia coli. J Struct Biol. 2000;132:133-41 pubmed
    ..This property may partially explain the contradiction of glycerol diffusion but limited water permeation capacity. ..
  56. Hahn E, Wild P, Hermanns U, Sebbel P, Glockshuber R, Häner M, et al. Exploring the 3D molecular architecture of Escherichia coli type 1 pili. J Mol Biol. 2002;323:845-57 pubmed
  57. Hilty C, Wider G, Fernández C, Wuthrich K. Stereospecific assignments of the isopropyl methyl groups of the membrane protein OmpX in DHPC micelles. J Biomol NMR. 2003;27:377-82 pubmed
  58. Fernández C, Wuthrich K. NMR solution structure determination of membrane proteins reconstituted in detergent micelles. FEBS Lett. 2003;555:144-50 pubmed
  59. Ahuja U, Thöny Meyer L. CcmD is involved in complex formation between CcmC and the heme chaperone CcmE during cytochrome c maturation. J Biol Chem. 2005;280:236-43 pubmed
    ..We postulate a function for CcmD in protein-protein interaction or membrane protein assembly required for the heme delivery process. ..
  60. Siegenthaler R, Christen P. The importance of having thermosensor control in the DnaK chaperone system. J Biol Chem. 2005;280:14395-401 pubmed
    ..Thus, the direct thermal adaptation of the DnaK chaperone system by thermosensing GrpE is essential for efficient chaperone action during heat shock. ..
  61. Grimshaw J, Siegenthaler R, Züger S, Schönfeld H, Z graggen B, Christen P. The heat-sensitive Escherichia coli grpE280 phenotype: impaired interaction of GrpE(G122D) with DnaK. J Mol Biol. 2005;353:888-96 pubmed
  62. DeClue M, Baldridge K, Künzler D, Kast P, Hilvert D. Isochorismate pyruvate lyase: a pericyclic reaction mechanism?. J Am Chem Soc. 2005;127:15002-3 pubmed
  63. Li X, Lupo D, Zheng L, Winkler F. Structural and functional insights into the AmtB/Mep/Rh protein family. Transfus Clin Biol. 2006;13:65-9 pubmed
    ..The next big challenge in the field surely is to determine the atomic structure of Rh proteins. ..
  64. Gossert A, Bettendorff P, Puorger C, Vetsch M, Herrmann T, Glockshuber R, et al. NMR structure of the Escherichia coli type 1 pilus subunit FimF and its interactions with other pilus subunits. J Mol Biol. 2008;375:752-63 pubmed
    ..They lend further support to the hypothesis that this flexibility would significantly increase the probability that the adhesin at the distal end of the fibrillum successfully targets host cell receptors...
  65. Ahuja U, Rozhkova A, Glockshuber R, Thöny Meyer L, Einsle O. Helix swapping leads to dimerization of the N-terminal domain of the c-type cytochrome maturation protein CcmH from Escherichia coli. FEBS Lett. 2008;582:2779-86 pubmed publisher
    ..We propose that an altered environment of the functional motif may help to discern between the two redox partners CcmG and apocytochrome c. ..
  66. Korkhov V, Mireku S, Hvorup R, Locher K. Asymmetric states of vitamin B?? transporter BtuCD are not discriminated by its cognate substrate binding protein BtuF. FEBS Lett. 2012;586:972-6 pubmed publisher
    ..The structure suggests that BtuF does not discriminate between, or impose, asymmetric conformations of BtuCD. It also explains the conformational disorder observed in BtuCDF crystals...
  67. Malojcic G, Owen R, Glockshuber R. Structural and mechanistic insights into the PAPS-independent sulfotransfer catalyzed by bacterial aryl sulfotransferase and the role of the DsbL/Dsbl system in its folding. Biochemistry. 2014;53:1870-7 pubmed publisher
    ..The conservation of paralogous dithiol oxidases with different substrate specificities in certain bacterial strains may therefore be a consequence of the complex folding pathways of their substrate proteins. ..
  68. Belevich N, von Ballmoos C, Verkhovskaya M. Activation of Proton Translocation by Respiratory Complex I. Biochemistry. 2017;56:5691-5697 pubmed publisher
    ..We conclude that Complex I undergoes a conversion from a decoupled state to a coupled state upon activation. The possible origins and importance of the observed phenomenon are discussed...
  69. Déthiollaz S, Eichenberger P, Geiselmann J. Influence of DNA geometry on transcriptional activation in Escherichia coli. EMBO J. 1996;15:5449-58 pubmed
  70. Prilipov A, Phale P, Koebnik R, Widmer C, Rosenbusch J. Identification and characterization of two quiescent porin genes, nmpC and ompN, in Escherichia coli BE. J Bacteriol. 1998;180:3388-92 pubmed
    ..coli K-12. The second DNA fragment detected corresponds to the nmpC gene, which, due to an insertion of an IS1 element in its coding region, is not expressed in E. coli BE. ..
  71. Ringler P, Borgnia M, Stahlberg H, Maloney P, Agre P, Engel A. Structure of the water channel AqpZ from Escherichia coli revealed by electron crystallography. J Mol Biol. 1999;291:1181-90 pubmed
    ..The 8 A projection map of the AqpZ tetramer in frozen hydrated samples is similar to that of AQP1, consistent with the high sequence homology between these proteins. ..
  72. Bothmann H, Pluckthun A. The periplasmic Escherichia coli peptidylprolyl cis,trans-isomerase FkpA. I. Increased functional expression of antibody fragments with and without cis-prolines. J Biol Chem. 2000;275:17100-5 pubmed
    ..Together with the in vitro data in the accompanying paper (Ramm, K., and Plückthun, A. (2000) J. Biol. Chem. 275, 17106-17113), we conclude that the effect of FkpA is independent of its PPIase activity. ..
  73. Phale P, Philippsen A, Widmer C, Phale V, Rosenbusch J, Schirmer T. Role of charged residues at the OmpF porin channel constriction probed by mutagenesis and simulation. Biochemistry. 2001;40:6319-25 pubmed
    ..This demonstrates that ion translocation through porin is mainly governed by pore geometry and charge, the two factors that are properly represented in the simulations. ..
  74. Van Gelder P, Dutzler R, Dumas F, Koebnik R, Schirmer T. Sucrose transport through maltoporin mutants of Escherichia coli. Protein Eng. 2001;14:943-8 pubmed
    ..Binding, however, was observed for the double mutant that had the obstructing residues truncated to alanines. ..
  75. Lacour S, Kolb A, Boris Zehnder A, Landini P. Mechanism of specific recognition of the aidB promoter by sigma(S)-RNA polymerase. Biochem Biophys Res Commun. 2002;292:922-30 pubmed
  76. Garcia Alles L, Siebold C, Nyffeler T, Flükiger Brühwiler K, Schneider P, Bürgi H, et al. Phosphoenolpyruvate- and ATP-dependent dihydroxyacetone kinases: covalent substrate-binding and kinetic mechanism. Biochemistry. 2004;43:13037-45 pubmed
    ..The Dha kinase remains fully active in the presence of 2 M glycerol, and phosphorylates trace impurities of carbonyl compounds present in glycerol. ..
  77. Ahuja U, Thöny Meyer L. The membrane anchors of the heme chaperone CcmE and the periplasmic thioredoxin CcmG are functionally important. FEBS Lett. 2006;580:216-22 pubmed
    ..However, the low level of holo-CcmE formed in the chimera is transferred efficiently to cytochrome c, indicating that heme delivery from CcmE does not involve the membrane anchor. ..
  78. Obrist M, Milek S, Klauck E, Hengge R, Narberhaus F. Region 2.1 of the Escherichia coli heat-shock sigma factor RpoH (sigma32) is necessary but not sufficient for degradation by the FtsH protease. Microbiology. 2007;153:2560-71 pubmed
    ..The in vivo stability of the mutated RpoH proteins correlated with their stability in a purified in vitro degradation system, suggesting that region 2.1 might be directly involved in the interaction with the FtsH protease. ..
  79. Puorger C, Eidam O, Capitani G, Erilov D, Grutter M, Glockshuber R. Infinite kinetic stability against dissociation of supramolecular protein complexes through donor strand complementation. Structure. 2008;16:631-42 pubmed publisher
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