Genomes and Genes
Experts and Doctors on escherichia coli proteins in Germany
Topic: escherichia coli proteins
Publications348 found, 100 shown here
- Wenz C, Rüfer A. Microchip CGE linked to immunoprecipitation as an alternative to Western blotting. Electrophoresis. 2009;30:4264-9 pubmed publisher..However, the new approach compares favorably to Western blotting in terms of time-to-result, usability and labor intensity, antibody consumption and access to quantitative data. ..
- Kolmsee T, Delic D, Agyenim T, Calles C, Wagner R. Differential stringent control of Escherichia coli rRNA promoters: effects of ppGpp, DksA and the initiating nucleotides. Microbiology. 2011;157:2871-9 pubmed publisher..Our results support the notion that the seven rRNA operons are differentially regulated and underline the importance of a concerted activity between ppGpp, DksA and an adequate concentration of the respective iNTP. ..
- Fulda M, Heinz E, Wolter F. The fadD gene of Escherichia coli K12 is located close to rnd at 39.6 min of the chromosomal map and is a new member of the AMP-binding protein family. Mol Gen Genet. 1994;242:241-9 pubmed..This family is extended by several new members and subdivided into four groups. fadD is assigned to a subgroup that does not include long chain acyl-CoA synthetases from eukaryotic organisms. ..
- Hippler B, Homuth G, Hoffmann T, Hungerer C, Schumann W, Jahn D. Characterization of Bacillus subtilis hemN. J Bacteriol. 1997;179:7181-5 pubmed..subtilis was obtained. Moreover, B. subtilis hemY did not substitute for hemF hemN deficiency in S. typhimurium. These results indicate the absence of hemF and suggest the presence of a second hemN-like gene in B. subtilis. ..
- Welz D, Braun V. Ferric citrate transport of Escherichia coli: functional regions of the FecR transmembrane regulatory protein. J Bacteriol. 1998;180:2387-94 pubmed
- Jung H, Rübenhagen R, Tebbe S, Leifker K, Tholema N, Quick M, et al. Topology of the Na+/proline transporter of Escherichia coli. J Biol Chem. 1998;273:26400-7 pubmed..The 13-helix structure is discussed as a common topological motif for all members of the Na+/solute cotransporter family. ..
- Brunkhorst C, Andersen C, Schneider E. Acarbose, a pseudooligosaccharide, is transported but not metabolized by the maltose-maltodextrin system of Escherichia coli. J Bacteriol. 1999;181:2612-9 pubmed..Acarbose could not be utilized as a carbon source since the compound alone was not a substrate of amylomaltase (MalQ) and was only poorly attacked by maltodextrin glucosidase (MalZ)...
- Thanbichler M, Bock A. Selenoprotein biosynthesis: purification and assay of components involved in selenocysteine biosynthesis and insertion in Escherichia coli. Methods Enzymol. 2002;347:3-16 pubmed
- Braun N, Meining W, Hars U, Fischer M, Ladenstein R, Huber R, et al. Formation of metal nanoclusters on specific surface sites of protein molecules. J Mol Biol. 2002;321:341-53 pubmed
- Bonin I, Mühlberger R, Bourenkov G, Huber R, Bacher A, Richter G, et al. Structural basis for the interaction of Escherichia coli NusA with protein N of phage lambda. Proc Natl Acad Sci U S A. 2004;101:13762-7 pubmed..Contrary to the RNA polymerase alpha subunit, lambdaN binding does not stimulate RNA interaction of NusA. The results demonstrate that lambdaN serves as a scaffold to closely oppose NusA and the mRNA in antitermination complexes. ..
- Li G, Laturnus C, Ewers C, Wieler L. Identification of genes required for avian Escherichia coli septicemia by signature-tagged mutagenesis. Infect Immun. 2005;73:2818-27 pubmed..This genome-wide analysis has identified both novel and previously known factors potentially involved in pathogenesis of APEC infection. ..
- Bertz M, Rief M. Mechanical unfoldons as building blocks of maltose-binding protein. J Mol Biol. 2008;378:447-58 pubmed publisher..We showed that many MBP constructs composed of those building blocks indeed form stably folded structures in solution. Mechanical unfoldons may provide a new tool for a systematic search for stable substructures of large proteins. ..
- Hullmann J, Patzer S, Römer C, Hantke K, Braun V. Periplasmic chaperone FkpA is essential for imported colicin M toxicity. Mol Microbiol. 2008;69:926-37 pubmed publisher..Colicin M is a suitable tool for the isolation of FkpA mutants used to elucidate the functions of the FkpA N- and C-domains. ..
- Cristovao M, Sisamakis E, Hingorani M, Marx A, Jung C, Rothwell P, et al. Single-molecule multiparameter fluorescence spectroscopy reveals directional MutS binding to mismatched bases in DNA. Nucleic Acids Res. 2012;40:5448-64 pubmed publisher..These findings shed light on prerequisites for MutS interactions with other MMR proteins for repairing the appropriate DNA strand. ..
- Fleck O, Michael H, Heim L. The swi4+ gene of Schizosaccharomyces pombe encodes a homologue of mismatch repair enzymes. Nucleic Acids Res. 1992;20:2271-8 pubmed..A strain with a disrupted swi4+ gene was constructed and analysed with respect to the switching process. As in swi4 mutants duplications occur in the mating-type region of the swi4 (null) strain, reducing the efficiency of switching. ..
- Sankar T, Neelakanta G, Sangal V, Plum G, Achtman M, Schnetz K. Fate of the H-NS-repressed bgl operon in evolution of Escherichia coli. PLoS Genet. 2009;5:e1000405 pubmed publisher..This suggests horizontal transfer of bgl genes to an ancestral Enterobacterium. Conservation and weak expression of bgl in isolates of phylogenetic group B2 may indicate a functional role of bgl in extraintestinal pathogenic E. coli. ..
- Gorlich D, Prehn S, Hartmann E, Kalies K, Rapoport T. A mammalian homolog of SEC61p and SECYp is associated with ribosomes and nascent polypeptides during translocation. Cell. 1992;71:489-503 pubmed..These results define Sec61p as a ubiquitous key component of the protein translocation apparatus. ..
- Richter G, Volk R, Krieger C, Lahm H, Rothlisberger U, Bacher A. Biosynthesis of riboflavin: cloning, sequencing, and expression of the gene coding for 3,4-dihydroxy-2-butanone 4-phosphate synthase of Escherichia coli. J Bacteriol. 1992;174:4050-6 pubmed..Sequence homology indicates that gene luxH of Vibrio harveyi and the central open reading frame of the Bacillus subtilis riboflavin operon code for 3,4-dihydroxy-2-butanone 4-phosphate synthase. ..
- Kaiser M, Sawers G. Overlapping promoters modulate Fnr- and ArcA-dependent anaerobic transcriptional activation of the focApfl operon in Escherichia coli. Microbiology. 1997;143 ( Pt 3):775-83 pubmed..Taken together, these results indicate that the P6A promoter moderates the Fnr-dependent activation of P6 through competition for RNA polymerase binding. ..
- Schafer U, Beck K, Muller M. Skp, a molecular chaperone of gram-negative bacteria, is required for the formation of soluble periplasmic intermediates of outer membrane proteins. J Biol Chem. 1999;274:24567-74 pubmed..Collectively, our data demonstrate that Skp is a molecular chaperone involved in generating and maintaining the solubility of early folding intermediates of outer membrane proteins in the periplasmic space of Gram-negative bacteria. ..
- Stallkamp I, Dowhan W, Altendorf K, Jung K. Negatively charged phospholipids influence the activity of the sensor kinase KdpD of Escherichia coli. Arch Microbiol. 1999;172:295-302 pubmed..These results indicate that electrostatic interactions are important for the activity of KdpD. ..
- Tran Q, Arras T, Becker S, Holighaus G, Ohlberger G, Unden G. Role of glutathione in the formation of the active form of the oxygen sensor FNR ([4Fe-4S].FNR) and in the control of FNR function. Eur J Biochem. 2000;267:4817-24 pubmed..FNR in vitro suggest an important role for glutathione in the de novo assembly of FNR and in the reductive activation of air-oxidized FNR under anaerobic conditions. ..
- Berry S, Schneider D, Vermaas W, Rögner M. Electron transport routes in whole cells of Synechocystis sp. strain PCC 6803: the role of the cytochrome bd-type oxidase. Biochemistry. 2002;41:3422-9 pubmed..In contrast, no indication of quinol oxidase activity in thylakoids was found for a second alternative oxidase encoded by the ctaII genes. ..
- Schwarzer D, Mootz H, Linne U, Marahiel M. Regeneration of misprimed nonribosomal peptide synthetases by type II thioesterases. Proc Natl Acad Sci U S A. 2002;99:14083-8 pubmed
- Pop O, Westermann M, Volkmer Engert R, Schulz D, Lemke C, Schreiber S, et al. Sequence-specific binding of prePhoD to soluble TatAd indicates protein-mediated targeting of the Tat export in Bacillus subtilis. J Biol Chem. 2003;278:38428-36 pubmed..These features suggest that TatA interacts prior to membrane integration with its pre-protein substrate and could therefore assist targeting of twin-arginine pre-proteins. ..
- Weibezahn J, Tessarz P, Schlieker C, Zahn R, Maglica Z, Lee S, et al. Thermotolerance requires refolding of aggregated proteins by substrate translocation through the central pore of ClpB. Cell. 2004;119:653-65 pubmed..The activity switch of BAP to a degrading disaggregase does not support thermotolerance development, demonstrating that cell survival during severe thermal stress requires reactivation of aggregated proteins. ..
- Wegrzyn R, Hofmann D, Merz F, Nikolay R, Rauch T, Graf C, et al. A conserved motif is prerequisite for the interaction of NAC with ribosomal protein L23 and nascent chains. J Biol Chem. 2006;281:2847-57 pubmed..We conclude that NAC employs a conserved ribosome binding domain to position itself on the L23 ribosomal protein adjacent to the nascent polypeptide exit site. ..
- Bredemeier R, Schlegel T, Ertel F, Vojta A, Borissenko L, Bohnsack M, et al. Functional and phylogenetic properties of the pore-forming beta-barrel transporters of the Omp85 family. J Biol Chem. 2007;282:1882-90 pubmed..Based on functional and phylogenetic analysis, we suggest an evolutionary scenario that explains the origin of the contemporary translocon. ..
- Lakshmipathy S, Tomic S, Kaiser C, Chang H, Genevaux P, Georgopoulos C, et al. Identification of nascent chain interaction sites on trigger factor. J Biol Chem. 2007;282:12186-93 pubmed..Our data indicate the existence of two regions on TF along which nascent chains can interact, the NC-domains as the main site and the PPIase domain as an auxiliary site. ..
- Kalamorz F, Reichenbach B, März W, Rak B, Görke B. Feedback control of glucosamine-6-phosphate synthase GlmS expression depends on the small RNA GlmZ and involves the novel protein YhbJ in Escherichia coli. Mol Microbiol. 2007;65:1518-33 pubmed..As in yhbJ mutants GlcN-6-P has no effect, YhbJ is essential for sensing this metabolite. ..
- Holzapfel E, Moser M, Schiltz E, Ueda T, Betton J, Muller M. Twin-arginine-dependent translocation of SufI in the absence of cytosolic helper proteins. Biochemistry. 2009;48:5096-105 pubmed publisher..coli was found to occur in the bona fide absence of any cytosolic chaperone. Our results suggest that in E. coli, cytosolic chaperones are not essential for the twin-arginine-dependent export of cofactor-less substrates. ..
- Jakob R, Zoldak G, Aumüller T, Schmid F. Chaperone domains convert prolyl isomerases into generic catalysts of protein folding. Proc Natl Acad Sci U S A. 2009;106:20282-7 pubmed publisher..The catalytic performance of these folding enzymes is thus determined by generic substrate recognition at the chaperone domain and efficient transfer to the active site in the prolyl isomerase domain. ..
- Lorenz S, Jakob R, Weininger U, Balbach J, Dobbek H, Schmid F. The filamentous phages fd and IF1 use different mechanisms to infect Escherichia coli. J Mol Biol. 2011;405:989-1003 pubmed publisher..Apparently, these closely related filamentous phages evolved different mechanisms to reconcile robustness with high infectivity. ..
- Koch D, Chan A, Murphy M, Lilie H, Grass G, Nies D. Characterization of a dipartite iron uptake system from uropathogenic Escherichia coli strain F11. J Biol Chem. 2011;286:25317-30 pubmed publisher..Together, these data indicate that FetMP is an additional iron uptake system composed of a putative iron permease and an iron-scavenging and potentially iron-reducing periplasmic protein. ..
- Hopper S, Babst M, Schlensog V, Fischer H, Hennecke H, Bock A. Regulated expression in vitro of genes coding for formate hydrogenlyase components of Escherichia coli. J Biol Chem. 1994;269:19597-604 pubmed..A supramolecular transcription complex is proposed which involves the binding of IHF to a site located between the UAS and the promoter responsible for transcription of the hyc operon. ..
- Voelkner P, Puppe W, Altendorf K. Characterization of the KdpD protein, the sensor kinase of the K(+)-translocating Kdp system of Escherichia coli. Eur J Biochem. 1993;217:1019-26 pubmed..Furthermore, replacement of the conserved histidine (His673), the predicted phosphorylation site in KdpD, by glutamine revealed that phosphorylation of KdpD was no longer possible. ..
- Schneider R, Travers A, Kutateladze T, Muskhelishvili G. A DNA architectural protein couples cellular physiology and DNA topology in Escherichia coli. Mol Microbiol. 1999;34:953-64 pubmed..We propose that FIS is involved in coupling cellular physiology to the topology of the bacterial chromosome. ..
- Diederichs K, Diez J, Greller G, Muller C, Breed J, Schnell C, et al. Crystal structure of MalK, the ATPase subunit of the trehalose/maltose ABC transporter of the archaeon Thermococcus litoralis. EMBO J. 2000;19:5951-61 pubmed..The structure and its relationship to function are discussed in the light of known mutations from the homologous Escherichia coli and Salmonella typhimurium proteins...
- Worbs M, Bourenkov G, Bartunik H, Huber R, Wahl M. An extended RNA binding surface through arrayed S1 and KH domains in transcription factor NusA. Mol Cell. 2001;7:1177-89 pubmed..Structural and mutational analyses indicate that the motifs cooperate, modulating strength and specificity of RNA binding...
- Mayans O, Ivens A, Nissen L, Kirschner K, Wilmanns M. Structural analysis of two enzymes catalysing reverse metabolic reactions implies common ancestry. EMBO J. 2002;21:3245-54 pubmed..These relationships establish new links for enzymes involved in nucleotide and amino acid metabolism. ..
- Bramkamp M, Altendorf K. Mutational analysis of charged residues in the putative KdpB-TM5 domain of the Kdp-ATPase of Escherichia coli. Ann N Y Acad Sci. 2003;986:351-3 pubmed
- Berthelmann F, Brüser T. Localization of the Tat translocon components in Escherichia coli. FEBS Lett. 2004;569:82-8 pubmed..We suggest that the active translocon complexes are mainly present at polar positions in Escherichia coli. ..
- Friedrich T, Stolpe S, Schneider D, Barquera B, Hellwig P. Ion translocation by the Escherichia coli NADH:ubiquinone oxidoreductase (complex I). Biochem Soc Trans. 2005;33:836-9 pubmed..We did not find any indications for Na+ translocation by the E. coli complex I. ..
- Scholz C, Eckert B, Hagn F, Schaarschmidt P, Balbach J, Schmid F. SlyD proteins from different species exhibit high prolyl isomerase and chaperone activities. Biochemistry. 2006;45:20-33 pubmed..The SlyD proteins are also well suited for biotechnological applications. As fusion partners they facilitate the refolding and increase the solubility of aggregation-prone proteins such as the gp41 ectodomain fragment of HIV-1. ..
- Schlapschy M, Grimm S, Skerra A. A system for concomitant overexpression of four periplasmic folding catalysts to improve secretory protein production in Escherichia coli. Protein Eng Des Sel. 2006;19:385-90 pubmed..Hence, pTUM4 represents a novel helper vector which complements existing cytosolic chaperone coexpression plasmids and should be useful for the functional secretion of various recombinant proteins with hampered folding efficiency. ..
- Farré E, Tech S, Trethewey R, Fernie A, Willmitzer L. Subcellular pyrophosphate metabolism in developing tubers of potato (Solanum tuberosum). Plant Mol Biol. 2006;62:165-79 pubmed..These results are discussed both with respect to our previous hypotheses and to current models of the compartmentation of potato tuber metabolism...
- Weber A, Kögl S, Jung K. Time-dependent proteome alterations under osmotic stress during aerobic and anaerobic growth in Escherichia coli. J Bacteriol. 2006;188:7165-75 pubmed..Only a few proteins were found to be down-regulated upon osmotic stress. ..
- Pul U, Wurm R, Wagner R. The role of LRP and H-NS in transcription regulation: involvement of synergism, allostery and macromolecular crowding. J Mol Biol. 2007;366:900-15 pubmed..The formation of this regulatory structure appears to be directly affected by environmental changes. ..
- Schultz T, Liu J, Capasso P, de Marco A. The solubility of recombinant proteins expressed in Escherichia coli is increased by otsA and otsB co-transformation. Biochem Biophys Res Commun. 2007;355:234-9 pubmed..Di-myo-inositol1,1'-phosphate (DIP) seems to be a good candidate to test in in vivo applications, although the opportunity of using otsA/B overexpressing cells is simpler and less expensive. ..
- Lehner I, Basting D, Meyer B, Haase W, Manolikas T, Kaiser C, et al. The key residue for substrate transport (Glu14) in the EmrE dimer is asymmetric. J Biol Chem. 2008;283:3281-8 pubmed..Upon addition of ethidium bromide, chemical shift changes and altered line shapes were observed, demonstrating substrate coordination by both Glu(14) in the dimer. ..
- Valeva A, Siegel I, Wylenzek M, Wassenaar T, Weis S, Heinz N, et al. Putative identification of an amphipathic alpha-helical sequence in hemolysin of Escherichia coli (HlyA) involved in transmembrane pore formation. Biol Chem. 2008;389:1201-7 pubmed publisher..Disruption of the helix structure did not affect binding properties, but totally abolished the hemolytic activity of the molecule. ..
- Berger M, Farcas A, Geertz M, Zhelyazkova P, Brix K, Travers A, et al. Coordination of genomic structure and transcription by the main bacterial nucleoid-associated protein HU. EMBO Rep. 2010;11:59-64 pubmed publisher..Our data thus implicate HU in coordinating the global genomic structure and function by regulating the spatial distribution of RNA polymerase in the nucleoid. ..
- Jahreis K, Postma P, Lengeler J. Nucleotide sequence of the ilvH-fruR gene region of Escherichia coli K12 and Salmonella typhimurium LT2. Mol Gen Genet. 1991;226:332-6 pubmed..coli shl gene (mnemonic for suppressor-H-linked phenotype). It is flanked upstream by the ilvIH genes and downstream by the pbpB gene in both organisms and by orfB, a gene possibly involved in the regulation of cell division. ..
- Rossmann R, Maier T, Lottspeich F, Bock A. Characterisation of a protease from Escherichia coli involved in hydrogenase maturation. Eur J Biochem. 1995;227:545-50 pubmed..Homologous genes are present in other transcriptional units coding for hydrogenases. ..
- Becker J, Brendel M. Molecular characterization of the xerC gene of Lactobacillus leichmannii encoding a site-specific recombinase and two adjacent heat shock genes. Curr Microbiol. 1996;32:232-6 pubmed..HtpO heat shock proteins. The deduced amino acid sequence of the fourth, incomplete ORF upstream the xerC gene showed strong homology with the gid A gene product of B. subtilis. ..
- Hengge Aronis R. Back to log phase: sigma S as a global regulator in the osmotic control of gene expression in Escherichia coli. Mol Microbiol. 1996;21:887-93 pubmed..Taken together, sigma S function is clearly not confined to stationary phase. Rather, sigma S may be regarded as a sigma factor associated with general stress conditions. ..
- Pfeifer A, Aszodi A, Seidler U, Ruth P, Hofmann F, Fassler R. Intestinal secretory defects and dwarfism in mice lacking cGMP-dependent protein kinase II. Science. 1996;274:2082-6 pubmed..The cGKII-deficient mice also developed dwarfism that was caused by a severe defect in endochondral ossification at the growth plates. These results indicate that cGKII plays a central role in diverse physiological processes. ..
- Mogk A, Tomoyasu T, Goloubinoff P, Rudiger S, Roder D, Langen H, et al. Identification of thermolabile Escherichia coli proteins: prevention and reversion of aggregation by DnaK and ClpB. EMBO J. 1999;18:6934-49 pubmed..chaperones/heat-shock response/Hsp70/protein denaturation/thermotolerance ..
- Reichmann P, Hakenbeck R. A XerD recombinase with unusual active site motifs in Streptococcus pneumoniae. J Mol Microbiol Biotechnol. 2002;4:101-10 pubmed..The xerD and xerC genes were present in a sample of 20 S. pneumoniae strains whereas the other xer genes appear to be absent in some of the strains and are more closely related to integrases of phage and transposon origin...
- Blume A, Chen H, Reutter W, Schmidt R, Hinderlich S. 2',3'-Dialdehydo-UDP-N-acetylglucosamine inhibits UDP-N-acetylglucosamine 2-epimerase, the key enzyme of sialic acid biosynthesis. FEBS Lett. 2002;521:127-32 pubmed..This compound may therefore represent a basis for the synthesis of potent inhibitors of UDP-N-acetylglucosamine 2-epimerase and, as a consequence, of the biosynthesis of sialic acids...
- Killmann H, Gestwa G. Determination of ferrichrome binding to the FhuA outer membrane transport protein, periplasmic accumulation of ferrichrome, or transport of ferrichrome into cells using a three-layer oil technique. Anal Biochem. 2002;310:55-60 pubmed..Similar transport rates were obtained with the two methods, but binding of ferrichrome to the mutated FhuA proteins and accumulation of ferrichrome in the periplasm could be measured only with the new method...
- Bramkamp M, Gassel M, Altendorf K. FITC binding site and p-nitrophenyl phosphatase activity of the Kdp-ATPase of Escherichia coli. Biochemistry. 2004;43:4559-67 pubmed..The notion that FITC inhibits pNPPase and ATPase activity supports the idea that the catalytic domain of KdpB is much more compact than other P-type ATPases, like Na(+),K(+)-ATPase, H(+),K(+)-ATPase, and Ca(2+)-ATPase. ..
- Chevalier N, Moser M, Koch H, Schimz K, Willery E, Locht C, et al. Membrane targeting of a bacterial virulence factor harbouring an extended signal peptide. J Mol Microbiol Biotechnol. 2004;8:7-18 pubmed..Our results rule out that the extended signal peptide of FHA determines a specific mode of membrane targeting but rather suggest that it might influence the export rate at the inner membrane. ..
- Manelyte L, Urbanke C, Giron Monzon L, Friedhoff P. Structural and functional analysis of the MutS C-terminal tetramerization domain. Nucleic Acids Res. 2006;34:5270-9 pubmed..Our data support the hypothesis that tetramerization of MutS is important but not essential for MutS function in MMR. ..
- Forzi L, Sawers R. Maturation of [NiFe]-hydrogenases in Escherichia coli. Biometals. 2007;20:565-78 pubmed..What is becoming increasingly clear is that the metabolic origins of the carbonyl group may be different. ..
- Weiss S, Ladwein M, Schmidt D, Ehinger J, Lommel S, Städing K, et al. IRSp53 links the enterohemorrhagic E. coli effectors Tir and EspFU for actin pedestal formation. Cell Host Microbe. 2009;5:244-58 pubmed publisher..Genetic and functional loss of IRSp53 abrogates actin assembly mediated by EHEC. Collectively, these data indentify IRSp53 family proteins as the missing host cell factors linking bacterial Tir and EspF(U) in EHEC pedestal formation. ..
- Lindenstrauss U, Matos C, Graubner W, Robinson C, Brüser T. Malfolded recombinant Tat substrates are Tat-independently degraded in Escherichia coli. FEBS Lett. 2010;584:3644-8 pubmed publisher..Malfolded NrfC is degraded more quickly than the native protein, indicating that Tat-independent protease systems can recognize malfolded Tat substrates. ..
- Hizlan D, Robson A, Whitehouse S, Gold V, Vonck J, Mills D, et al. Structure of the SecY complex unlocked by a preprotein mimic. Cell Rep. 2012;1:21-8 pubmed..Therefore, we propose that the structure describes the translocon unlocked by preprotein and poised for protein translocation. ..
- Hunke S, Mourez M, Jehanno M, Dassa E, Schneider E. ATP modulates subunit-subunit interactions in an ATP-binding cassette transporter (MalFGK2) determined by site-directed chemical cross-linking. J Biol Chem. 2000;275:15526-34 pubmed..These interactions are strongly modulated by MgATP, indicating a structural rearrangement of the subunits during the transport cycle. These data are discussed with respect to current transport models...
- Wollmann P, Zeth K. The structure of RseB: a sensor in periplasmic stress response of E. coli. J Mol Biol. 2007;372:927-41 pubmed..On the basis of the structure of RseB, we suggest that it acts as a sensor of periplasmic stress with a dual functionality: it detects mislocalized lipoproteins and propagates the signal to induce the sigma(E)-response. ..
- Puppe W, Zimmann P, Jung K, Lucassen M, Altendorf K. Characterization of truncated forms of the KdpD protein, the sensor kinase of the K+-translocating Kdp system of Escherichia coli. J Biol Chem. 1996;271:25027-34 pubmed..Furthermore, kdp expression was investigated using a KdpA-LacZ fusion. The data obtained support the notion that the extent of kdp expression is modulated by the N-terminal part of KdpD. ..
- Grininger M, Ravelli R, Heider U, Zeth K. Expression, crystallization and crystallographic analysis of DegS, a stress sensor of the bacterial periplasm. Acta Crystallogr D Biol Crystallogr. 2004;60:1429-31 pubmed..The correctness of the molecular-replacement solution was verified by identifying radiation-damage-induced structural changes. ..
- Olkhova E, Hunte C, Screpanti E, Padan E, Michel H. Multiconformation continuum electrostatics analysis of the NhaA Na+/H+ antiporter of Escherichia coli with functional implications. Proc Natl Acad Sci U S A. 2006;103:2629-34 pubmed..The results of the calculations provide valuable information on the activation of the antiporter and the role of individual amino acid residues, and provide a solid framework for further experiments. ..
- Fröbel J, Rose P, Lausberg F, Blümmel A, Freudl R, Muller M. Transmembrane insertion of twin-arginine signal peptides is driven by TatC and regulated by TatB. Nat Commun. 2012;3:1311 pubmed publisher..Collectively our results suggest that TatC is an insertase for twin-arginine signal peptides and that translocation-proficient signal sequence recognition requires the concerted action of TatC and TatB. ..
- Voegele R, Sweet G, Boos W. Glycerol kinase of Escherichia coli is activated by interaction with the glycerol facilitator. J Bacteriol. 1993;175:1087-94 pubmed..We conclude that there is an interaction between the glycerol facilitator protein and glycerol kinase that stimulates kinase activity, analogous to the hexokinase- and glycerol kinase-porin interactions in mitochondria. ..
- Koch H, Hengelage T, Neumann Haefelin C, Macfarlane J, Hoffschulte H, Schimz K, et al. In vitro studies with purified components reveal signal recognition particle (SRP) and SecA/SecB as constituents of two independent protein-targeting pathways of Escherichia coli. Mol Biol Cell. 1999;10:2163-73 pubmed..coli. Both targeting pathways intersect at the translocation pore because they are equally affected by a blocked translocation channel. ..
- Leskovar A, Reinstein J. Photophysical properties of popular fluorescent adenosine nucleotide analogs used in enzyme mechanism probing. Arch Biochem Biophys. 2008;473:16-24 pubmed publisher..The measured quantum yields quantify the increase in fluorescence for (C8)-MABA-ADP, MANT-ATP and (Pgamma)-MABA-ATP as 153%, 93% and 14% when bound to DnaK, ClpB and Trap1, respectively, compared to free in buffer solution. ..
- Bruder J, Siewert K, Obermeier B, Malotka J, Scheinert P, Kellermann J, et al. Target specificity of an autoreactive pathogenic human ??-T cell receptor in myositis. J Biol Chem. 2012;287:20986-95 pubmed publisher..The observation that AA-RS are targeted by a ??-T cell and by autoantibodies reveals an unexpected link between T cell and antibody responses in autoimmune myositis. ..
- Horstkorte R, Nöhring S, Danker K, Effertz K, Reutter W, Lucka L. Protein kinase C phosphorylates and regulates UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase. FEBS Lett. 2000;470:315-8 pubmed..Furthermore, PKC phosphorylates UDP-GlcNAc 2-epimerase and this phosphorylation results in an upregulation of the UDP-GlcNAc 2-epimerase enzyme activity. ..
- Hantke K. Members of the Fur protein family regulate iron and zinc transport in E. coli and characteristics of the Fur-regulated fhuF protein. J Mol Microbiol Biotechnol. 2002;4:217-22 pubmed..Only two additional Zur binding sites in the promoter region of genes with unknown function were found. Properties of Zur and Fur proteins from different bacteria are compared. ..
- Reissmann S, Hochleitner E, Wang H, Paschos A, Lottspeich F, Glass R, et al. Taming of a poison: biosynthesis of the NiFe-hydrogenase cyanide ligands. Science. 2003;299:1067-70 pubmed..This finding underscores a striking parallel between biochemistry and organometallic chemistry in the formation of an iron-cyano complex...
- Fournier E, Nikolaev A, Nasiri H, Hoeser J, Friedrich T, Hellwig P, et al. Creation of a gold nanoparticle based electrochemical assay for the detection of inhibitors of bacterial cytochrome bd oxidases. Bioelectrochemistry. 2016;111:109-14 pubmed publisher..After optimization of the protein coverages, the assay shows at pH7 a good reproducibility and readout stability over time, and it is thus suitable for further screening of small molecule collections. ..
- Schlegl J, Fürste J, Bald R, Erdmann V, Hartmann R. Cleavage efficiencies of model substrates for ribonuclease P from Escherichia coli and Thermus thermophilus. Nucleic Acids Res. 1992;20:5963-70 pubmed..In addition, acceptor stem--T arm substrates, composed of two synthetic RNA fragments, have been designed to mimic internal cleavage of any target RNA molecule available for base pairing. ..
- Brunder W, Schmidt H, Karch H. EspP, a novel extracellular serine protease of enterohaemorrhagic Escherichia coli O157:H7 cleaves human coagulation factor V. Mol Microbiol. 1997;24:767-78 pubmed..Functional analysis showed that EspP is a protease capable of cleaving pepsin A and human coagulation factor V. Degradation of factor V could contribute to the mucosal haemorrhage observed in patients with haemorrhagic colitis. ..
- Leonhartsberger S, Ehrenreich A, Bock A. Analysis of the domain structure and the DNA binding site of the transcriptional activator FhlA. Eur J Biochem. 2000;267:3672-84 pubmed..The analysis of orthologs of FhlA revealed that they are able to functionally replace the E. coli enzyme. ..
- Drazic A, Miura H, Peschek J, Le Y, Bach N, Kriehuber T, et al. Methionine oxidation activates a transcription factor in response to oxidative stress. Proc Natl Acad Sci U S A. 2013;110:9493-8 pubmed publisher..Inactivation of HypT depends on the methionine sulfoxide reductases A/B. Thus, microbial protection mechanisms have evolved along the evolution of antimicrobial control systems, allowing bacteria to survive within the host environment...
- Gösringer M, Lechner M, Brillante N, Weber C, Rossmanith W, Hartmann R. Protein-only RNase P function in Escherichia coli: viability, processing defects and differences between PRORP isoenzymes. Nucleic Acids Res. 2017;45:7441-7454 pubmed publisher..The cells' viability also suggests that the essential function of the signal recognition particle can be maintained with a 5?-extended 4.5S RNA. ..
- Roth A, Messer W. The DNA binding domain of the initiator protein DnaA. EMBO J. 1995;14:2106-11 pubmed..coli. This fusion protein can be coupled directly to streptavidin-coated magnetic beads. Homologies between DnaA protein and transcription factors of the NtrC family are discussed. ..
- Rassow J, Dekker P, van Wilpe S, Meijer M, Soll J. The preprotein translocase of the mitochondrial inner membrane: function and evolution. J Mol Biol. 1999;286:105-20 pubmed