Experts and Doctors on escherichia coli proteins in Germany

Summary

Locale: Germany
Topic: escherichia coli proteins

Top Publications

  1. Muhlenhoff U. The FAPY-DNA glycosylase (Fpg) is required for survival of the cyanobacterium Synechococcus elongatus under high light irradiance. FEMS Microbiol Lett. 2000;187:127-32 pubmed
  2. Dudek J, Volkmer J, Bies C, Guth S, Müller A, Lerner M, et al. A novel type of co-chaperone mediates transmembrane recruitment of DnaK-like chaperones to ribosomes. EMBO J. 2002;21:2958-67 pubmed
  3. Lerm M, Pop M, Fritz G, Aktories K, Schmidt G. Proteasomal degradation of cytotoxic necrotizing factor 1-activated rac. Infect Immun. 2002;70:4053-8 pubmed
    ..Schmidt, Infect. Immun. 67:496-503, 1998). Here we show that CNF1-induced JNK activation is stabilized in the presence of lactacystin. The data indicate that Rac is degraded by a proteasome-dependent pathway in CNF1-treated cells. ..
  4. Kremling A, Heermann R, Centler F, Jung K, Gilles E. Analysis of two-component signal transduction by mathematical modeling using the KdpD/KdpE system of Escherichia coli. Biosystems. 2004;78:23-37 pubmed
    ..Hereby one submodel represents signal transduction while the second submodel describes the gene expression. ..
  5. Illarionova V, Kaiser J, Ostrozhenkova E, Bacher A, Fischer M, Eisenreich W, et al. Nonmevalonate terpene biosynthesis enzymes as antiinfective drug targets: substrate synthesis and high-throughput screening methods. J Org Chem. 2006;71:8824-34 pubmed
    ..The preparation of 4-diphosphosphocytidyl-2C-methyl-D-erythritol 2-phosphate in multigram quantities is described for the first time. ..
  6. Fleischer R, Heermann R, Jung K, Hunke S. Purification, reconstitution, and characterization of the CpxRAP envelope stress system of Escherichia coli. J Biol Chem. 2007;282:8583-93 pubmed
  7. Agarwal V, Fink U, Schuldiner S, Reif B. MAS solid-state NMR studies on the multidrug transporter EmrE. Biochim Biophys Acta. 2007;1768:3036-43 pubmed
    ..These experiments allow to assign the chemical shift of the carboxylic carbon of E14. In addition, spectra are analyzed which are obtained in the presence and absence of the ligand TPP+. ..
  8. Antonoaea R, Fürst M, Nishiyama K, Muller M. The periplasmic chaperone PpiD interacts with secretory proteins exiting from the SecYEG translocon. Biochemistry. 2008;47:5649-56 pubmed publisher
    ..Consistent with a coupling between translocation across the SecYEG translocon and folding by periplasmic chaperones, a lack of PpiD retards the release of a translocating outer membrane protein into the periplasm. ..
  9. Lüttmann D, Heermann R, Zimmer B, Hillmann A, Rampp I, Jung K, et al. Stimulation of the potassium sensor KdpD kinase activity by interaction with the phosphotransferase protein IIA(Ntr) in Escherichia coli. Mol Microbiol. 2009;72:978-94 pubmed publisher
    ..Taken together, the data show that the Ntr-PTS has an important role in maintaining K+ homeostasis and links K+ uptake to carbohydrate metabolism. ..

More Information

Publications348 found, 100 shown here

  1. Wenz C, Rüfer A. Microchip CGE linked to immunoprecipitation as an alternative to Western blotting. Electrophoresis. 2009;30:4264-9 pubmed publisher
    ..However, the new approach compares favorably to Western blotting in terms of time-to-result, usability and labor intensity, antibody consumption and access to quantitative data. ..
  2. Kolmsee T, Delic D, Agyenim T, Calles C, Wagner R. Differential stringent control of Escherichia coli rRNA promoters: effects of ppGpp, DksA and the initiating nucleotides. Microbiology. 2011;157:2871-9 pubmed publisher
    ..Our results support the notion that the seven rRNA operons are differentially regulated and underline the importance of a concerted activity between ppGpp, DksA and an adequate concentration of the respective iNTP. ..
  3. Fulda M, Heinz E, Wolter F. The fadD gene of Escherichia coli K12 is located close to rnd at 39.6 min of the chromosomal map and is a new member of the AMP-binding protein family. Mol Gen Genet. 1994;242:241-9 pubmed
    ..This family is extended by several new members and subdivided into four groups. fadD is assigned to a subgroup that does not include long chain acyl-CoA synthetases from eukaryotic organisms. ..
  4. Hippler B, Homuth G, Hoffmann T, Hungerer C, Schumann W, Jahn D. Characterization of Bacillus subtilis hemN. J Bacteriol. 1997;179:7181-5 pubmed
    ..subtilis was obtained. Moreover, B. subtilis hemY did not substitute for hemF hemN deficiency in S. typhimurium. These results indicate the absence of hemF and suggest the presence of a second hemN-like gene in B. subtilis. ..
  5. Welz D, Braun V. Ferric citrate transport of Escherichia coli: functional regions of the FecR transmembrane regulatory protein. J Bacteriol. 1998;180:2387-94 pubmed
  6. Jung H, Rübenhagen R, Tebbe S, Leifker K, Tholema N, Quick M, et al. Topology of the Na+/proline transporter of Escherichia coli. J Biol Chem. 1998;273:26400-7 pubmed
    ..The 13-helix structure is discussed as a common topological motif for all members of the Na+/solute cotransporter family. ..
  7. Brunkhorst C, Andersen C, Schneider E. Acarbose, a pseudooligosaccharide, is transported but not metabolized by the maltose-maltodextrin system of Escherichia coli. J Bacteriol. 1999;181:2612-9 pubmed
    ..Acarbose could not be utilized as a carbon source since the compound alone was not a substrate of amylomaltase (MalQ) and was only poorly attacked by maltodextrin glucosidase (MalZ)...
  8. Thanbichler M, Bock A. Selenoprotein biosynthesis: purification and assay of components involved in selenocysteine biosynthesis and insertion in Escherichia coli. Methods Enzymol. 2002;347:3-16 pubmed
  9. Braun N, Meining W, Hars U, Fischer M, Ladenstein R, Huber R, et al. Formation of metal nanoclusters on specific surface sites of protein molecules. J Mol Biol. 2002;321:341-53 pubmed
  10. Bonin I, Mühlberger R, Bourenkov G, Huber R, Bacher A, Richter G, et al. Structural basis for the interaction of Escherichia coli NusA with protein N of phage lambda. Proc Natl Acad Sci U S A. 2004;101:13762-7 pubmed
    ..Contrary to the RNA polymerase alpha subunit, lambdaN binding does not stimulate RNA interaction of NusA. The results demonstrate that lambdaN serves as a scaffold to closely oppose NusA and the mRNA in antitermination complexes. ..
  11. Li G, Laturnus C, Ewers C, Wieler L. Identification of genes required for avian Escherichia coli septicemia by signature-tagged mutagenesis. Infect Immun. 2005;73:2818-27 pubmed
    ..This genome-wide analysis has identified both novel and previously known factors potentially involved in pathogenesis of APEC infection. ..
  12. Bertz M, Rief M. Mechanical unfoldons as building blocks of maltose-binding protein. J Mol Biol. 2008;378:447-58 pubmed publisher
    ..We showed that many MBP constructs composed of those building blocks indeed form stably folded structures in solution. Mechanical unfoldons may provide a new tool for a systematic search for stable substructures of large proteins. ..
  13. Hullmann J, Patzer S, Römer C, Hantke K, Braun V. Periplasmic chaperone FkpA is essential for imported colicin M toxicity. Mol Microbiol. 2008;69:926-37 pubmed publisher
    ..Colicin M is a suitable tool for the isolation of FkpA mutants used to elucidate the functions of the FkpA N- and C-domains. ..
  14. Cristovao M, Sisamakis E, Hingorani M, Marx A, Jung C, Rothwell P, et al. Single-molecule multiparameter fluorescence spectroscopy reveals directional MutS binding to mismatched bases in DNA. Nucleic Acids Res. 2012;40:5448-64 pubmed publisher
    ..These findings shed light on prerequisites for MutS interactions with other MMR proteins for repairing the appropriate DNA strand. ..
  15. Fleck O, Michael H, Heim L. The swi4+ gene of Schizosaccharomyces pombe encodes a homologue of mismatch repair enzymes. Nucleic Acids Res. 1992;20:2271-8 pubmed
    ..A strain with a disrupted swi4+ gene was constructed and analysed with respect to the switching process. As in swi4 mutants duplications occur in the mating-type region of the swi4 (null) strain, reducing the efficiency of switching. ..
  16. Sankar T, Neelakanta G, Sangal V, Plum G, Achtman M, Schnetz K. Fate of the H-NS-repressed bgl operon in evolution of Escherichia coli. PLoS Genet. 2009;5:e1000405 pubmed publisher
    ..This suggests horizontal transfer of bgl genes to an ancestral Enterobacterium. Conservation and weak expression of bgl in isolates of phylogenetic group B2 may indicate a functional role of bgl in extraintestinal pathogenic E. coli. ..
  17. Gorlich D, Prehn S, Hartmann E, Kalies K, Rapoport T. A mammalian homolog of SEC61p and SECYp is associated with ribosomes and nascent polypeptides during translocation. Cell. 1992;71:489-503 pubmed
    ..These results define Sec61p as a ubiquitous key component of the protein translocation apparatus. ..
  18. Richter G, Volk R, Krieger C, Lahm H, Rothlisberger U, Bacher A. Biosynthesis of riboflavin: cloning, sequencing, and expression of the gene coding for 3,4-dihydroxy-2-butanone 4-phosphate synthase of Escherichia coli. J Bacteriol. 1992;174:4050-6 pubmed
    ..Sequence homology indicates that gene luxH of Vibrio harveyi and the central open reading frame of the Bacillus subtilis riboflavin operon code for 3,4-dihydroxy-2-butanone 4-phosphate synthase. ..
  19. Kaiser M, Sawers G. Overlapping promoters modulate Fnr- and ArcA-dependent anaerobic transcriptional activation of the focApfl operon in Escherichia coli. Microbiology. 1997;143 ( Pt 3):775-83 pubmed
    ..Taken together, these results indicate that the P6A promoter moderates the Fnr-dependent activation of P6 through competition for RNA polymerase binding. ..
  20. Schafer U, Beck K, Muller M. Skp, a molecular chaperone of gram-negative bacteria, is required for the formation of soluble periplasmic intermediates of outer membrane proteins. J Biol Chem. 1999;274:24567-74 pubmed
    ..Collectively, our data demonstrate that Skp is a molecular chaperone involved in generating and maintaining the solubility of early folding intermediates of outer membrane proteins in the periplasmic space of Gram-negative bacteria. ..
  21. Stallkamp I, Dowhan W, Altendorf K, Jung K. Negatively charged phospholipids influence the activity of the sensor kinase KdpD of Escherichia coli. Arch Microbiol. 1999;172:295-302 pubmed
    ..These results indicate that electrostatic interactions are important for the activity of KdpD. ..
  22. Tran Q, Arras T, Becker S, Holighaus G, Ohlberger G, Unden G. Role of glutathione in the formation of the active form of the oxygen sensor FNR ([4Fe-4S].FNR) and in the control of FNR function. Eur J Biochem. 2000;267:4817-24 pubmed
    ..FNR in vitro suggest an important role for glutathione in the de novo assembly of FNR and in the reductive activation of air-oxidized FNR under anaerobic conditions. ..
  23. Berry S, Schneider D, Vermaas W, Rögner M. Electron transport routes in whole cells of Synechocystis sp. strain PCC 6803: the role of the cytochrome bd-type oxidase. Biochemistry. 2002;41:3422-9 pubmed
    ..In contrast, no indication of quinol oxidase activity in thylakoids was found for a second alternative oxidase encoded by the ctaII genes. ..
  24. Schwarzer D, Mootz H, Linne U, Marahiel M. Regeneration of misprimed nonribosomal peptide synthetases by type II thioesterases. Proc Natl Acad Sci U S A. 2002;99:14083-8 pubmed
  25. Pop O, Westermann M, Volkmer Engert R, Schulz D, Lemke C, Schreiber S, et al. Sequence-specific binding of prePhoD to soluble TatAd indicates protein-mediated targeting of the Tat export in Bacillus subtilis. J Biol Chem. 2003;278:38428-36 pubmed
    ..These features suggest that TatA interacts prior to membrane integration with its pre-protein substrate and could therefore assist targeting of twin-arginine pre-proteins. ..
  26. Weibezahn J, Tessarz P, Schlieker C, Zahn R, Maglica Z, Lee S, et al. Thermotolerance requires refolding of aggregated proteins by substrate translocation through the central pore of ClpB. Cell. 2004;119:653-65 pubmed
    ..The activity switch of BAP to a degrading disaggregase does not support thermotolerance development, demonstrating that cell survival during severe thermal stress requires reactivation of aggregated proteins. ..
  27. Wegrzyn R, Hofmann D, Merz F, Nikolay R, Rauch T, Graf C, et al. A conserved motif is prerequisite for the interaction of NAC with ribosomal protein L23 and nascent chains. J Biol Chem. 2006;281:2847-57 pubmed
    ..We conclude that NAC employs a conserved ribosome binding domain to position itself on the L23 ribosomal protein adjacent to the nascent polypeptide exit site. ..
  28. Bredemeier R, Schlegel T, Ertel F, Vojta A, Borissenko L, Bohnsack M, et al. Functional and phylogenetic properties of the pore-forming beta-barrel transporters of the Omp85 family. J Biol Chem. 2007;282:1882-90 pubmed
    ..Based on functional and phylogenetic analysis, we suggest an evolutionary scenario that explains the origin of the contemporary translocon. ..
  29. Lakshmipathy S, Tomic S, Kaiser C, Chang H, Genevaux P, Georgopoulos C, et al. Identification of nascent chain interaction sites on trigger factor. J Biol Chem. 2007;282:12186-93 pubmed
    ..Our data indicate the existence of two regions on TF along which nascent chains can interact, the NC-domains as the main site and the PPIase domain as an auxiliary site. ..
  30. Kalamorz F, Reichenbach B, März W, Rak B, Görke B. Feedback control of glucosamine-6-phosphate synthase GlmS expression depends on the small RNA GlmZ and involves the novel protein YhbJ in Escherichia coli. Mol Microbiol. 2007;65:1518-33 pubmed
    ..As in yhbJ mutants GlcN-6-P has no effect, YhbJ is essential for sensing this metabolite. ..
  31. Holzapfel E, Moser M, Schiltz E, Ueda T, Betton J, Muller M. Twin-arginine-dependent translocation of SufI in the absence of cytosolic helper proteins. Biochemistry. 2009;48:5096-105 pubmed publisher
    ..coli was found to occur in the bona fide absence of any cytosolic chaperone. Our results suggest that in E. coli, cytosolic chaperones are not essential for the twin-arginine-dependent export of cofactor-less substrates. ..
  32. Jakob R, Zoldak G, Aumüller T, Schmid F. Chaperone domains convert prolyl isomerases into generic catalysts of protein folding. Proc Natl Acad Sci U S A. 2009;106:20282-7 pubmed publisher
    ..The catalytic performance of these folding enzymes is thus determined by generic substrate recognition at the chaperone domain and efficient transfer to the active site in the prolyl isomerase domain. ..
  33. Lorenz S, Jakob R, Weininger U, Balbach J, Dobbek H, Schmid F. The filamentous phages fd and IF1 use different mechanisms to infect Escherichia coli. J Mol Biol. 2011;405:989-1003 pubmed publisher
    ..Apparently, these closely related filamentous phages evolved different mechanisms to reconcile robustness with high infectivity. ..
  34. Koch D, Chan A, Murphy M, Lilie H, Grass G, Nies D. Characterization of a dipartite iron uptake system from uropathogenic Escherichia coli strain F11. J Biol Chem. 2011;286:25317-30 pubmed publisher
    ..Together, these data indicate that FetMP is an additional iron uptake system composed of a putative iron permease and an iron-scavenging and potentially iron-reducing periplasmic protein. ..
  35. Hopper S, Babst M, Schlensog V, Fischer H, Hennecke H, Bock A. Regulated expression in vitro of genes coding for formate hydrogenlyase components of Escherichia coli. J Biol Chem. 1994;269:19597-604 pubmed
    ..A supramolecular transcription complex is proposed which involves the binding of IHF to a site located between the UAS and the promoter responsible for transcription of the hyc operon. ..
  36. Voelkner P, Puppe W, Altendorf K. Characterization of the KdpD protein, the sensor kinase of the K(+)-translocating Kdp system of Escherichia coli. Eur J Biochem. 1993;217:1019-26 pubmed
    ..Furthermore, replacement of the conserved histidine (His673), the predicted phosphorylation site in KdpD, by glutamine revealed that phosphorylation of KdpD was no longer possible. ..
  37. Schneider R, Travers A, Kutateladze T, Muskhelishvili G. A DNA architectural protein couples cellular physiology and DNA topology in Escherichia coli. Mol Microbiol. 1999;34:953-64 pubmed
    ..We propose that FIS is involved in coupling cellular physiology to the topology of the bacterial chromosome. ..
  38. Diederichs K, Diez J, Greller G, Muller C, Breed J, Schnell C, et al. Crystal structure of MalK, the ATPase subunit of the trehalose/maltose ABC transporter of the archaeon Thermococcus litoralis. EMBO J. 2000;19:5951-61 pubmed
    ..The structure and its relationship to function are discussed in the light of known mutations from the homologous Escherichia coli and Salmonella typhimurium proteins...
  39. Worbs M, Bourenkov G, Bartunik H, Huber R, Wahl M. An extended RNA binding surface through arrayed S1 and KH domains in transcription factor NusA. Mol Cell. 2001;7:1177-89 pubmed
    ..Structural and mutational analyses indicate that the motifs cooperate, modulating strength and specificity of RNA binding...
  40. Mayans O, Ivens A, Nissen L, Kirschner K, Wilmanns M. Structural analysis of two enzymes catalysing reverse metabolic reactions implies common ancestry. EMBO J. 2002;21:3245-54 pubmed
    ..These relationships establish new links for enzymes involved in nucleotide and amino acid metabolism. ..
  41. Bramkamp M, Altendorf K. Mutational analysis of charged residues in the putative KdpB-TM5 domain of the Kdp-ATPase of Escherichia coli. Ann N Y Acad Sci. 2003;986:351-3 pubmed
  42. Berthelmann F, Brüser T. Localization of the Tat translocon components in Escherichia coli. FEBS Lett. 2004;569:82-8 pubmed
    ..We suggest that the active translocon complexes are mainly present at polar positions in Escherichia coli. ..
  43. Friedrich T, Stolpe S, Schneider D, Barquera B, Hellwig P. Ion translocation by the Escherichia coli NADH:ubiquinone oxidoreductase (complex I). Biochem Soc Trans. 2005;33:836-9 pubmed
    ..We did not find any indications for Na+ translocation by the E. coli complex I. ..
  44. Scholz C, Eckert B, Hagn F, Schaarschmidt P, Balbach J, Schmid F. SlyD proteins from different species exhibit high prolyl isomerase and chaperone activities. Biochemistry. 2006;45:20-33 pubmed
    ..The SlyD proteins are also well suited for biotechnological applications. As fusion partners they facilitate the refolding and increase the solubility of aggregation-prone proteins such as the gp41 ectodomain fragment of HIV-1. ..
  45. Schlapschy M, Grimm S, Skerra A. A system for concomitant overexpression of four periplasmic folding catalysts to improve secretory protein production in Escherichia coli. Protein Eng Des Sel. 2006;19:385-90 pubmed
    ..Hence, pTUM4 represents a novel helper vector which complements existing cytosolic chaperone coexpression plasmids and should be useful for the functional secretion of various recombinant proteins with hampered folding efficiency. ..
  46. Farré E, Tech S, Trethewey R, Fernie A, Willmitzer L. Subcellular pyrophosphate metabolism in developing tubers of potato (Solanum tuberosum). Plant Mol Biol. 2006;62:165-79 pubmed
    ..These results are discussed both with respect to our previous hypotheses and to current models of the compartmentation of potato tuber metabolism...
  47. Weber A, Kögl S, Jung K. Time-dependent proteome alterations under osmotic stress during aerobic and anaerobic growth in Escherichia coli. J Bacteriol. 2006;188:7165-75 pubmed
    ..Only a few proteins were found to be down-regulated upon osmotic stress. ..
  48. Pul U, Wurm R, Wagner R. The role of LRP and H-NS in transcription regulation: involvement of synergism, allostery and macromolecular crowding. J Mol Biol. 2007;366:900-15 pubmed
    ..The formation of this regulatory structure appears to be directly affected by environmental changes. ..
  49. Schultz T, Liu J, Capasso P, de Marco A. The solubility of recombinant proteins expressed in Escherichia coli is increased by otsA and otsB co-transformation. Biochem Biophys Res Commun. 2007;355:234-9 pubmed
    ..Di-myo-inositol1,1'-phosphate (DIP) seems to be a good candidate to test in in vivo applications, although the opportunity of using otsA/B overexpressing cells is simpler and less expensive. ..
  50. Lehner I, Basting D, Meyer B, Haase W, Manolikas T, Kaiser C, et al. The key residue for substrate transport (Glu14) in the EmrE dimer is asymmetric. J Biol Chem. 2008;283:3281-8 pubmed
    ..Upon addition of ethidium bromide, chemical shift changes and altered line shapes were observed, demonstrating substrate coordination by both Glu(14) in the dimer. ..
  51. Valeva A, Siegel I, Wylenzek M, Wassenaar T, Weis S, Heinz N, et al. Putative identification of an amphipathic alpha-helical sequence in hemolysin of Escherichia coli (HlyA) involved in transmembrane pore formation. Biol Chem. 2008;389:1201-7 pubmed publisher
    ..Disruption of the helix structure did not affect binding properties, but totally abolished the hemolytic activity of the molecule. ..
  52. Berger M, Farcas A, Geertz M, Zhelyazkova P, Brix K, Travers A, et al. Coordination of genomic structure and transcription by the main bacterial nucleoid-associated protein HU. EMBO Rep. 2010;11:59-64 pubmed publisher
    ..Our data thus implicate HU in coordinating the global genomic structure and function by regulating the spatial distribution of RNA polymerase in the nucleoid. ..
  53. Lehr U, Schutz M, Oberhettinger P, Ruiz Perez F, Donald J, Palmer T, et al. C-terminal amino acid residues of the trimeric autotransporter adhesin YadA of Yersinia enterocolitica are decisive for its recognition and assembly by BamA. Mol Microbiol. 2010;78:932-46 pubmed publisher
  54. Martos A, Jimenez M, Rivas G, Schwille P. Towards a bottom-up reconstitution of bacterial cell division. Trends Cell Biol. 2012;22:634-43 pubmed publisher
  55. Jahreis K, Postma P, Lengeler J. Nucleotide sequence of the ilvH-fruR gene region of Escherichia coli K12 and Salmonella typhimurium LT2. Mol Gen Genet. 1991;226:332-6 pubmed
    ..coli shl gene (mnemonic for suppressor-H-linked phenotype). It is flanked upstream by the ilvIH genes and downstream by the pbpB gene in both organisms and by orfB, a gene possibly involved in the regulation of cell division. ..
  56. Rossmann R, Maier T, Lottspeich F, Bock A. Characterisation of a protease from Escherichia coli involved in hydrogenase maturation. Eur J Biochem. 1995;227:545-50 pubmed
    ..Homologous genes are present in other transcriptional units coding for hydrogenases. ..
  57. Becker J, Brendel M. Molecular characterization of the xerC gene of Lactobacillus leichmannii encoding a site-specific recombinase and two adjacent heat shock genes. Curr Microbiol. 1996;32:232-6 pubmed
    ..HtpO heat shock proteins. The deduced amino acid sequence of the fourth, incomplete ORF upstream the xerC gene showed strong homology with the gid A gene product of B. subtilis. ..
  58. Hengge Aronis R. Back to log phase: sigma S as a global regulator in the osmotic control of gene expression in Escherichia coli. Mol Microbiol. 1996;21:887-93 pubmed
    ..Taken together, sigma S function is clearly not confined to stationary phase. Rather, sigma S may be regarded as a sigma factor associated with general stress conditions. ..
  59. Pfeifer A, Aszodi A, Seidler U, Ruth P, Hofmann F, Fassler R. Intestinal secretory defects and dwarfism in mice lacking cGMP-dependent protein kinase II. Science. 1996;274:2082-6 pubmed
    ..The cGKII-deficient mice also developed dwarfism that was caused by a severe defect in endochondral ossification at the growth plates. These results indicate that cGKII plays a central role in diverse physiological processes. ..
  60. Mogk A, Tomoyasu T, Goloubinoff P, Rudiger S, Roder D, Langen H, et al. Identification of thermolabile Escherichia coli proteins: prevention and reversion of aggregation by DnaK and ClpB. EMBO J. 1999;18:6934-49 pubmed
    ..chaperones/heat-shock response/Hsp70/protein denaturation/thermotolerance ..
  61. Reichmann P, Hakenbeck R. A XerD recombinase with unusual active site motifs in Streptococcus pneumoniae. J Mol Microbiol Biotechnol. 2002;4:101-10 pubmed
    ..The xerD and xerC genes were present in a sample of 20 S. pneumoniae strains whereas the other xer genes appear to be absent in some of the strains and are more closely related to integrases of phage and transposon origin...
  62. Blume A, Chen H, Reutter W, Schmidt R, Hinderlich S. 2',3'-Dialdehydo-UDP-N-acetylglucosamine inhibits UDP-N-acetylglucosamine 2-epimerase, the key enzyme of sialic acid biosynthesis. FEBS Lett. 2002;521:127-32 pubmed
    ..This compound may therefore represent a basis for the synthesis of potent inhibitors of UDP-N-acetylglucosamine 2-epimerase and, as a consequence, of the biosynthesis of sialic acids...
  63. Killmann H, Gestwa G. Determination of ferrichrome binding to the FhuA outer membrane transport protein, periplasmic accumulation of ferrichrome, or transport of ferrichrome into cells using a three-layer oil technique. Anal Biochem. 2002;310:55-60 pubmed
    ..Similar transport rates were obtained with the two methods, but binding of ferrichrome to the mutated FhuA proteins and accumulation of ferrichrome in the periplasm could be measured only with the new method...
  64. Bramkamp M, Gassel M, Altendorf K. FITC binding site and p-nitrophenyl phosphatase activity of the Kdp-ATPase of Escherichia coli. Biochemistry. 2004;43:4559-67 pubmed
    ..The notion that FITC inhibits pNPPase and ATPase activity supports the idea that the catalytic domain of KdpB is much more compact than other P-type ATPases, like Na(+),K(+)-ATPase, H(+),K(+)-ATPase, and Ca(2+)-ATPase. ..
  65. Chevalier N, Moser M, Koch H, Schimz K, Willery E, Locht C, et al. Membrane targeting of a bacterial virulence factor harbouring an extended signal peptide. J Mol Microbiol Biotechnol. 2004;8:7-18 pubmed
    ..Our results rule out that the extended signal peptide of FHA determines a specific mode of membrane targeting but rather suggest that it might influence the export rate at the inner membrane. ..
  66. Manelyte L, Urbanke C, Giron Monzon L, Friedhoff P. Structural and functional analysis of the MutS C-terminal tetramerization domain. Nucleic Acids Res. 2006;34:5270-9 pubmed
    ..Our data support the hypothesis that tetramerization of MutS is important but not essential for MutS function in MMR. ..
  67. Forzi L, Sawers R. Maturation of [NiFe]-hydrogenases in Escherichia coli. Biometals. 2007;20:565-78 pubmed
    ..What is becoming increasingly clear is that the metabolic origins of the carbonyl group may be different. ..
  68. Weiss S, Ladwein M, Schmidt D, Ehinger J, Lommel S, Städing K, et al. IRSp53 links the enterohemorrhagic E. coli effectors Tir and EspFU for actin pedestal formation. Cell Host Microbe. 2009;5:244-58 pubmed publisher
    ..Genetic and functional loss of IRSp53 abrogates actin assembly mediated by EHEC. Collectively, these data indentify IRSp53 family proteins as the missing host cell factors linking bacterial Tir and EspF(U) in EHEC pedestal formation. ..
  69. Lindenstrauss U, Matos C, Graubner W, Robinson C, Brüser T. Malfolded recombinant Tat substrates are Tat-independently degraded in Escherichia coli. FEBS Lett. 2010;584:3644-8 pubmed publisher
    ..Malfolded NrfC is degraded more quickly than the native protein, indicating that Tat-independent protease systems can recognize malfolded Tat substrates. ..
  70. Eicher T, Cha H, Seeger M, Brandstätter L, El Delik J, Bohnert J, et al. Transport of drugs by the multidrug transporter AcrB involves an access and a deep binding pocket that are separated by a switch-loop. Proc Natl Acad Sci U S A. 2012;109:5687-92 pubmed publisher
  71. Hizlan D, Robson A, Whitehouse S, Gold V, Vonck J, Mills D, et al. Structure of the SecY complex unlocked by a preprotein mimic. Cell Rep. 2012;1:21-8 pubmed
    ..Therefore, we propose that the structure describes the translocon unlocked by preprotein and poised for protein translocation. ..
  72. Hunke S, Mourez M, Jehanno M, Dassa E, Schneider E. ATP modulates subunit-subunit interactions in an ATP-binding cassette transporter (MalFGK2) determined by site-directed chemical cross-linking. J Biol Chem. 2000;275:15526-34 pubmed
    ..These interactions are strongly modulated by MgATP, indicating a structural rearrangement of the subunits during the transport cycle. These data are discussed with respect to current transport models...
  73. Wollmann P, Zeth K. The structure of RseB: a sensor in periplasmic stress response of E. coli. J Mol Biol. 2007;372:927-41 pubmed
    ..On the basis of the structure of RseB, we suggest that it acts as a sensor of periplasmic stress with a dual functionality: it detects mislocalized lipoproteins and propagates the signal to induce the sigma(E)-response. ..
  74. Puppe W, Zimmann P, Jung K, Lucassen M, Altendorf K. Characterization of truncated forms of the KdpD protein, the sensor kinase of the K+-translocating Kdp system of Escherichia coli. J Biol Chem. 1996;271:25027-34 pubmed
    ..Furthermore, kdp expression was investigated using a KdpA-LacZ fusion. The data obtained support the notion that the extent of kdp expression is modulated by the N-terminal part of KdpD. ..
  75. Grininger M, Ravelli R, Heider U, Zeth K. Expression, crystallization and crystallographic analysis of DegS, a stress sensor of the bacterial periplasm. Acta Crystallogr D Biol Crystallogr. 2004;60:1429-31 pubmed
    ..The correctness of the molecular-replacement solution was verified by identifying radiation-damage-induced structural changes. ..
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