Genomes and Genes
Experts and Doctors on escherichia coli proteins in Denmark
Topic: escherichia coli proteins
Publications151 found, 100 shown here
- Løbner Olesen A, Skovgaard O, Marinus M. Dam methylation: coordinating cellular processes. Curr Opin Microbiol. 2005;8:154-60 pubmed..DamMT has also been implicated as a virulence factor in bacterial pathogenesis. The origin and phylogeny of DamMT, based on sequenced genomes, has been deduced. ..
- Andersen K, Wang H, Otzen D. A kinetic analysis of the folding and unfolding of OmpA in urea and guanidinium chloride: single and parallel pathways. Biochemistry. 2012;51:8371-83 pubmed publisher..However, BBP, a minimalist transmembrane β-barrel version of OmpA with shortened loops, is destabilized by ~10 kcal/mol compared to OmpA, highlighting loop contributions to OmpA stability. ..
- Gerdes K, Gultyaev A, Franch T, Pedersen K, Mikkelsen N. Antisense RNA-regulated programmed cell death. Annu Rev Genet. 1997;31:1-31 pubmed..The complex phylogenetic relationships of the plasmid- and chromosome-encoded systems are also presented and discussed. ..
- Kallipolitis B, Valentin Hansen P. Transcription of rpoH, encoding the Escherichia coli heat-shock regulator sigma32, is negatively controlled by the cAMP-CRP/CytR nucleoprotein complex. Mol Microbiol. 1998;29:1091-9 pubmed..These findings suggest that additional assistance in protein folding is required under conditions in which CytR is induced (i.e. in the presence of nucleosides). ..
- Zhu C, Loft S. Effects of Brussels sprouts extracts on hydrogen peroxide-induced DNA strand breaks in human lymphocytes. Food Chem Toxicol. 2001;39:1191-7 pubmed..The results indicate that compounds, including sinigrin, in cooked and autolysed Brussels sprouts can enhance lymphocyte resistance towards H2O2-induced DNA strand breaks in vitro. ..
- Atlung T, Nielsen H, Hansen F. Characterisation of the allelic variation in the rpoS gene in thirteen K12 and six other non-pathogenic Escherichia coli strains. Mol Genet Genomics. 2002;266:873-81 pubmed publisher..When cloned into a LacI-controlled vector and compared in a rpoS::Tn 10 background, the RpoS(33Q) and RpoS(33E) variants showed the same activity...
- Dueholm M, Nielsen S, Hein K, Nissen P, Chapman M, Christiansen G, et al. Fibrillation of the major curli subunit CsgA under a wide range of conditions implies a robust design of aggregation. Biochemistry. 2011;50:8281-90 pubmed publisher..Furthermore, we show that CsgA polymerization in vitro is preceded by the formation of thin needlelike protofibrils followed by aggregation of the amyloid fibrils. ..
- Nørregaard Madsen M, Mygind B, Pedersen R, Valentin Hansen P, Søgaard Andersen L. The gene encoding the periplasmic cyclophilin homologue, PPIase A, in Escherichia coli, is expressed from four promoters, three of which are activated by the cAMP-CRP complex and negatively regulated by the CytR repressor. Mol Microbiol. 1994;14:989-97 pubmed..These results expand the CytR regulon to include a gene whose product has no known function in ribo- and deoxyribonucleoside catabolism or transport. ..
- Andersen A, Hansen E. Cloning of the lysA gene from Mycobacterium tuberculosis. Gene. 1993;124:105-9 pubmed..The deduced amino acid sequence was found to exhibit significant homology (from 55% to 73% similarity, and from 27% to 53% identity) to DAP decarboxylase sequences from other bacterial species. ..
- Brøndsted L, Atlung T. Effect of growth conditions on expression of the acid phosphatase (cyx-appA) operon and the appY gene, which encodes a transcriptional activator of Escherichia coli. J Bacteriol. 1996;178:1556-64 pubmed..The alternative sigma factor sigma S has limited influence on the transcription of the appY gene during entry into stationary phase and no effect on the induction by phosphate starvation. ..
- Vogel U, Jensen K. NusA is required for ribosomal antitermination and for modulation of the transcription elongation rate of both antiterminated RNA and mRNA. J Biol Chem. 1997;272:12265-71 pubmed..These results suggest that NusA is the component of the transcription elongation complex required for inhibition of mRNA elongation by ppGpp. ..
- Hansen J, Gregersen N, Bross P. Differential degradation of variant medium-chain acyl-CoA dehydrogenase by the protein quality control proteases Lon and ClpXP. Biochem Biophys Res Commun. 2005;333:1160-70 pubmed..Our results suggest that Lon is more efficient than ClpXP in elimination of non-native MCAD protein conformations, and accordingly, that Lon can recognise a broader spectrum of MCAD protein conformations. ..
- Jensen M, Petersen G, Lauritzen C, Pedersen J, Led J. Metal binding sites in proteins: identification and characterization by paramagnetic NMR relaxation. Biochemistry. 2005;44:11014-23 pubmed..In addition, Ni(2+) binds specifically but more weakly to the surface-exposed side chain carboxylate groups of residues D10, D20, D47, and E85. ..
- Hansen F, Christensen B, Nielsen C, Atlung T. Insights into the quality of DnaA boxes and their cooperativity. J Mol Biol. 2006;355:85-95 pubmed..Parallel in vitro experiments using a purified DNA-binding domain of DnaA protein gave the same ranking of the binding affinities of the eight DnaA boxes. ..
- Vejborg R, Klemm P. Cellular chain formation in Escherichia coli biofilms. Microbiology. 2009;155:1407-17 pubmed publisher..Combined, these results illustrate the diversity of biofilm architectures that can be observed even within a single microbial species. ..
- D Alvise P, Magdenoska O, Melchiorsen J, Nielsen K, Gram L. Biofilm formation and antibiotic production in Ruegeria mobilis are influenced by intracellular concentrations of cyclic dimeric guanosinmonophosphate. Environ Microbiol. 2014;16:1252-66 pubmed publisher..In conclusion, c-di-GMP signalling controls biofilm formation and biofilm-associated traits in R.?mobilis and, as suggested by presence of GGDEF and EAL domain protein genes, also in other Roseobacter clade species. ..
- Lin L, Petersen T, Jensen K, Bolund L, Kühn R, Luo Y. Fusion of SpCas9 to E. coli Rec A protein enhances CRISPR-Cas9 mediated gene knockout in mammalian cells. J Biotechnol. 2017;247:42-49 pubmed publisher..This approach may form the basis for further investigations of the applications of recombinant Cas9 proteins to fine-tuning DSB repair pathways in eukaryotic cells. ..
- Birkelund S, Lundemose A, Christiansen G. The 75-kilodalton cytoplasmic Chlamydia trachomatis L2 polypeptide is a DnaK-like protein. Infect Immun. 1990;58:2098-104 pubmed..This mixed promoter was recognized in E. coli...
- Andersen P, Frees D, Fast R, Mygind B. Uracil uptake in Escherichia coli K-12: isolation of uraA mutants and cloning of the gene. J Bacteriol. 1995;177:2008-13 pubmed..The UraA protein has been identified in sodium dodecyl sulfate-polyacrylamide gels in the membrane fraction of minicells harboring the uraA plasmids. ..
- Hasman H, Schembri M, Klemm P. Antigen 43 and type 1 fimbriae determine colony morphology of Escherichia coli K-12. J Bacteriol. 2000;182:1089-95 pubmed..Interestingly, the habitat specificity of bacteria under static liquid conditions seems to correlate with the colony phenotypes. ..
- Rasmussen A, Eriksen M, Gilany K, Udesen C, Franch T, Petersen C, et al. Regulation of ompA mRNA stability: the role of a small regulatory RNA in growth phase-dependent control. Mol Microbiol. 2005;58:1421-9 pubmed..Our results indicate that distinct regulatory circuits are responsible for growth phase- and growth rate-dependent control of the ompA mRNA stability. ..
- Johansson E, Thymark M, Bynck J, Fanø M, Larsen S, Willemoes M. Regulation of dCTP deaminase from Escherichia coli by nonallosteric dTTP binding to an inactive form of the enzyme. FEBS J. 2007;274:4188-98 pubmed..The dCTP deaminase then resembles a simple concerted system subjected to effector binding, but without the use of an allosteric site. ..
- Kang L, Shaw A, Xu D, Xia W, Zhang J, Deng J, et al. Upregulation of MetC is essential for D-alanine-independent growth of an alr/dadX-deficient Escherichia coli strain. J Bacteriol. 2011;193:1098-106 pubmed publisher..coli mutants and provide evidence for significant alanine racemase coactivity of the E. coli cystathionine beta-lyase (MetC). ..
- Bøggild A, Sofos N, Andersen K, Feddersen A, Easter A, Passmore L, et al. The crystal structure of the intact E. coli RelBE toxin-antitoxin complex provides the structural basis for conditional cooperativity. Structure. 2012;20:1641-8 pubmed publisher..The results thus provide a firm basis for understanding the model of conditional cooperativity at the molecular level. ..
- Pedersen G, Jensen H, Schelde A, Toft C, Pedersen H, Ulrichsen M, et al. The basolateral vesicle sorting machinery and basolateral proteins are recruited to the site of enteropathogenic E. coli microcolony growth at the apical membrane. PLoS ONE. 2017;12:e0179122 pubmed publisher..This may aid EPEC attachment and subsequent microcolony growth. ..
- Atlung T, Brøndsted L. Role of the transcriptional activator AppY in regulation of the cyx appA operon of Escherichia coli by anaerobiosis, phosphate starvation, and growth phase. J Bacteriol. 1994;176:5414-22 pubmed..The possibility that AppY is a third global regulator of energy metabolism genes is discussed. ..
- Birn H, Verroust P, Nexo E, Hager H, Jacobsen C, Christensen E, et al. Characterization of an epithelial approximately 460-kDa protein that facilitates endocytosis of intrinsic factor-vitamin B12 and binds receptor-associated protein. J Biol Chem. 1997;272:26497-504 pubmed..In conclusion, we have identified the epithelial IF-B12-binding receptor as a approximately 460-kDa RAP-binding protein facilitating endocytosis. ..
- Purta E, O Connor M, Bujnicki J, Douthwaite S. YccW is the m5C methyltransferase specific for 23S rRNA nucleotide 1962. J Mol Biol. 2008;383:641-51 pubmed publisher..In keeping with the previously proposed nomenclature system for bacterial rRNA methyltransferases, yccW is now designated as the rRNA large subunit methyltransferase gene rlmI. ..
- Christiansen N, Nielsen L, Jakobsen L, Stegger M, Hansen L, Frimodt Møller N. Fluoroquinolone resistance mechanisms in urinary tract pathogenic Escherichia coli isolated during rapidly increasing fluoroquinolone consumption in a low-use country. Microb Drug Resist. 2011;17:395-406 pubmed publisher..In Denmark, increasing ciprofloxacin resistance in E. coli is mainly due to mutational events and not to spread of clones. ..
- Kristensen O, Gajhede M. Chaperone binding at the ribosomal exit tunnel. Structure. 2003;11:1547-56 pubmed..Comparison reveals that this feature is not found in SRP structures. We identify a conserved helical kink as a hallmark of the TF structure that is most likely critical to ensure ribosome association. ..
- Skals M, Jensen U, Ousingsawat J, Kunzelmann K, Leipziger J, Praetorius H. Escherichia coli alpha-hemolysin triggers shrinkage of erythrocytes via K(Ca)3.1 and TMEM16A channels with subsequent phosphatidylserine exposure. J Biol Chem. 2010;285:15557-65 pubmed publisher..This mechanism might potentially allow HlyA-damaged erythrocytes to be removed from the bloodstream by macrophages and thereby reduce the risk of intravascular hemolysis. ..
- Breum S, Boel J. Prevalence of Escherichia coli O157 and verocytotoxin producing E. coli (VTEC) on Danish beef carcasses. Int J Food Microbiol. 2010;141:90-6 pubmed publisher..The present study shows that real time PCR screening of carcass samples for genes encoding virulence or other genetic markers is a reliable method for rapid identification of carcasses that potentially are contaminated with VTEC. ..
- Damborg P, Marskar P, Baptiste K, Guardabassi L. Faecal shedding of CTX-M-producing Escherichia coli in horses receiving broad-spectrum antimicrobial prophylaxis after hospital admission. Vet Microbiol. 2012;154:298-304 pubmed publisher..The originality of the study lies in the unanticipated high frequency and genetic diversity of CTX-M-producing E. coli observed in the faecal flora of hospitalized patients receiving broad-spectrum antimicrobial prophylaxis. ..
- Weinert B, Scholz C, Wagner S, Iesmantavicius V, Su D, Daniel J, et al. Lysine succinylation is a frequently occurring modification in prokaryotes and eukaryotes and extensively overlaps with acetylation. Cell Rep. 2013;4:842-51 pubmed publisher..These data provide a systems-wide view of succinylation and its dynamic regulation and show its extensive overlap with acetylation. ..
- Hao N, Sneppen K, Shearwin K, Dodd I. Efficient chromosomal-scale DNA looping in Escherichia coli using multiple DNA-looping elements. Nucleic Acids Res. 2017;45:5074-5085 pubmed publisher..A combination of these approaches was able to generate efficient DNA looping at a 200 kb distance. ..
- Frees D, Qazi S, Hill P, Ingmer H. Alternative roles of ClpX and ClpP in Staphylococcus aureus stress tolerance and virulence. Mol Microbiol. 2003;48:1565-78 pubmed..Thus, our results indicate that ClpX and ClpP contribute to virulence by controlling the activity of major virulence factors rather than by promoting stress tolerance. ..
- Frees D, Chastanet A, Qazi S, S rensen K, Hill P, Msadek T, et al. Clp ATPases are required for stress tolerance, intracellular replication and biofilm formation in Staphylococcus aureus. Mol Microbiol. 2004;54:1445-62 pubmed publisher..Thus, our data show that Clp proteolytic complexes and the Clp ATPases control several key processes of importance to the success of S. aureus as a pathogen...
- Overgaard M, Borch J, Jørgensen M, Gerdes K. Messenger RNA interferase RelE controls relBE transcription by conditional cooperativity. Mol Microbiol. 2008;69:841-57 pubmed publisher..A mutational analysis of the operator sites showed that RelE in excess counteracted cooperative binding of the RelB(2)*RelE complexes to the operator sites. Thus, RelE controls relBE transcription by conditional cooperativity. ..
- Overgaard M, Borch J, Gerdes K. RelB and RelE of Escherichia coli form a tight complex that represses transcription via the ribbon-helix-helix motif in RelB. J Mol Biol. 2009;394:183-96 pubmed publisher..Lon degraded RelB in vitro and degradation was inhibited by RelE, consistent with the proposal that RelE protects RelB from proteolysis by Lon in vivo. ..
- Purta E, Kaminska K, Kasprzak J, Bujnicki J, Douthwaite S. YbeA is the m3Psi methyltransferase RlmH that targets nucleotide 1915 in 23S rRNA. RNA. 2008;14:2234-44 pubmed publisher..Methylation by YbeA (redesignated RlmH for rRNA large subunit methyltransferase H) possibly functions as a stamp of approval signifying that the 50S subunit has engaged in translational initiation. ..
- Sengeløv G, Sørensen S. Methods for detection of conjugative plasmid transfer in aquatic environments. Curr Microbiol. 1998;37:274-80 pubmed..Thus, this counter selection system was not usable in microcosm experiments. ..
- Stentebjerg Olesen B, Chakraborty T, Klemm P. FimE-catalyzed off-to-on inversion of the type 1 fimbrial phase switch and insertion sequence recruitment in an Escherichia coli K-12 fimB strain. FEMS Microbiol Lett. 2000;182:319-25 pubmed..Following inversion of the phase switch, this element can remain phase-locked in the on orientation due to integration of insertion sequence elements, viz. IS1 or IS5, at various positions in either the fimE gene or the phase switch. ..
- Ussery D, Larsen T, Wilkes K, Friis C, Worning P, Krogh A, et al. Genome organisation and chromatin structure in Escherichia coli. Biochimie. 2001;83:201-12 pubmed..We also show that the regions upstream of genes regulated by H-NS are more curved and have a higher AT content than regions upstream of other genes. These regions in general would also be localised near the replication terminus. ..
- Reinau M, Otzen D. Stability and structure of the membrane protein transporter Ffh is modulated by substrates and lipids. Arch Biochem Biophys. 2009;492:48-53 pubmed publisher..Binding is largely mediated by electrostatic interactions but does not protect Ffh against trypsinolysis. We conclude that Ffh is a structurally flexible and dynamic protein whose stability is significantly modulated by the environment. ..
- Kotzsch A, Vernet E, Hammarström M, Berthelsen J, Weigelt J, Gräslund S, et al. A secretory system for bacterial production of high-profile protein targets. Protein Sci. 2011;20:597-609 pubmed publisher..Using this high-throughput compatible system, we clearly demonstrate that secreted biomedically relevant human proteins can be efficiently retrieved and purified from the growth medium. ..
- Valentin Hansen P, Søgaard Andersen L, Pedersen H. A flexible partnership: the CytR anti-activator and the cAMP-CRP activator protein, comrades in transcription control. Mol Microbiol. 1996;20:461-6 pubmed
- Bourke Arnvig K, Pedersen S, Sneppen K. Thermodynamics of heat-shock response. Phys Rev Lett. 2000;84:3005-8 pubmed..This suggests that stability related to hot as well as cold unfolding of proteins is directly implemented in the biological control of protein folding. ..
- Kjaergaard K, Hasman H, Schembri M, Klemm P. Antigen 43-mediated autotransporter display, a versatile bacterial cell surface presentation system. J Bacteriol. 2002;184:4197-204 pubmed..Furthermore, Ag43 can be displayed in many gram-negative bacteria. This feature is exploited for display of our chimeras in an attenuated Salmonella strain. ..
- Christensen S, Pedersen K, Hansen F, Gerdes K. Toxin-antitoxin loci as stress-response-elements: ChpAK/MazF and ChpBK cleave translated RNAs and are counteracted by tmRNA. J Mol Biol. 2003;332:809-19 pubmed..Thus, ChpAK and ChpBK inhibit translation by a mechanism very similar to that of E.coli RelE. On the basis of these results, we propose a model that integrates TA loci into general prokaryotic stress physiology...
- Kruse T, Bork Jensen J, Gerdes K. The morphogenetic MreBCD proteins of Escherichia coli form an essential membrane-bound complex. Mol Microbiol. 2005;55:78-89 pubmed..Based on these and other observations we propose a model in which the membrane-associated MreBCD complex directs longitudinal cell wall synthesis in a process essential to maintain cell morphology. ..
- Bojer M, Jakobsen H, Struve C, Krogfelt K, L bner Olesen A. Lack of the RNA chaperone Hfq attenuates pathogenicity of several Escherichia coli pathotypes towards Caenorhabditis elegans. Microbes Infect. 2012;14:1034-9 pubmed publisher..Thus, Hfq is intrinsically essential to pathogenic E. coli for survival and virulence exerted in the C. elegans host...
- MÃ¸ller T, Overgaard M, Nielsen S, Bortolaia V, Sommer M, Guardabassi L, et al. Relation between tetR and tetA expression in tetracycline resistant Escherichia coli. BMC Microbiol. 2016;16:39 pubmed publisher..coli growth, tetracycline exposure and expression of tetracycline resistance. ..
- Schembri M, Hjerrild L, Gjermansen M, Klemm P. Differential expression of the Escherichia coli autoaggregation factor antigen 43. J Bacteriol. 2003;185:2236-42 pubmed..Furthermore, the redox state of OxyR influences the biofilm-forming potential of E. coli. Finally, we demonstrated that Ag43-mediated cell aggregation confers significant protection against hydrogen peroxide killing. ..
- Gerdes K, Nielsen A, Thorsted P, Wagner E. Mechanism of killer gene activation. Antisense RNA-dependent RNase III cleavage ensures rapid turn-over of the stable hok, srnB and pndA effector messenger RNAs. J Mol Biol. 1992;226:637-49 pubmed..Based on these and on observations obtained previously, we present a molecular model that explains the activation of the killer mRNAs in plasmid-free segregants and after addition of rifampicin. ..
- Pedersen H, Søgaard Andersen L, Holst B, Valentin Hansen P. Heterologous cooperativity in Escherichia coli. The CytR repressor both contacts DNA and the cAMP receptor protein when binding to the deoP2 promoter. J Biol Chem. 1991;266:17804-8 pubmed
- Jørgensen C, Dandanell G. Isolation and characterization of mutations in the Escherichia coli regulatory protein XapR. J Bacteriol. 1999;181:4397-403 pubmed..These regions, when modelled into the three-dimensional structure of CysB from Klebsiella aerogenes, are placed close together and are most probably directly involved in binding the inducer xanthosine. ..
- Mortensen L, Dandanell G, Hammer K. Purification and characterization of the deoR repressor of Escherichia coli. EMBO J. 1989;8:325-31 pubmed
- Nilsson D, Kilstrup M. Cloning and expression of the Lactococcus lactis purDEK genes, required for growth in milk. Appl Environ Microbiol. 1998;64:4321-7 pubmed..No secondary transcription start points were mapped in or close to this region, indicating that a putative activator site and not a promoter was deleted or partly destroyed. ..
- Thorslund T, Sunesen M, Bohr V, Stevnsner T. Repair of 8-oxoG is slower in endogenous nuclear genes than in mitochondrial DNA and is without strand bias. DNA Repair (Amst). 2002;1:261-73 pubmed..In vivo, mitochondrial DNA is highly exposed to reactive oxygen species (ROS), and we find that the repair of 8-oxoG is more efficient in the mitochondrial DNA than in the nuclear DNA. ..
- Riber L, Olsson J, Jensen R, Skovgaard O, Dasgupta S, Marinus M, et al. Hda-mediated inactivation of the DnaA protein and dnaA gene autoregulation act in concert to ensure homeostatic maintenance of the Escherichia coli chromosome. Genes Dev. 2006;20:2121-34 pubmed..Based on these observations, we propose that both RIDA and dnaA gene autoregulation are required as homeostatic mechanisms to ensure that initiation of replication occurs at the same time relative to cell mass in each cell cycle. ..
- Hancock V, Klemm P. Global gene expression profiling of asymptomatic bacteriuria Escherichia coli during biofilm growth in human urine. Infect Immun. 2007;75:966-76 pubmed..05). Also, the expression profile of strain 83972 in the human urinary tract partially overlaps with the biofilm expression profile...
- Johansen J, Eriksen M, Kallipolitis B, Valentin Hansen P. Down-regulation of outer membrane proteins by noncoding RNAs: unraveling the cAMP-CRP- and sigmaE-dependent CyaR-ompX regulatory case. J Mol Biol. 2008;383:1-9 pubmed publisher..The conservation of CyaR, MicA, RybB, and their targets suggests that the omp mRNA-sRNA regulatory network is an integral part of the envelope stress response in many enterobacteria. ..
- Maury J, Asadollahi M, Møller K, Schalk M, Clark A, Formenti L, et al. Reconstruction of a bacterial isoprenoid biosynthetic pathway in Saccharomyces cerevisiae. FEBS Lett. 2008;582:4032-8 pubmed publisher..cerevisiae. mRNA from the seven genes was detected, and the pathway was shown able to sustain growth of yeast in conditions of inhibition of its constitutive isoprenoid biosynthetic pathway. ..
- Zeng X, Saxild H, Switzer R. Purification and characterization of the DeoR repressor of Bacillus subtilis. J Bacteriol. 2000;182:1916-22 pubmed..DNase I footprinting exhibited a protected region that extends over more than 43 bp, covering a palindrome together with a direct repeat to one half of the palindrome and the nucleotides between them. ..
- Søgaard I, Boesen T, Mygind T, Melkova R, Birkelund S, Christiansen G, et al. Recombination in Mycoplasma hominis. Infect Genet Evol. 2002;1:277-85 pubmed..hominis and this may explain the high intraspecies variability. The results obtained in the present study may be of importance for future population studies of Mycoplasma species. ..
- Christensen S, Gerdes K. Delayed-relaxed response explained by hyperactivation of RelE. Mol Microbiol. 2004;53:587-97 pubmed..Restoration of the prestarvation level of ppGpp, in turn, allows for the resumption of stable RNA synthesis seen during the delayed-relaxed response. ..
- Sherlock O, Dobrindt U, Jensen J, Munk Vejborg R, Klemm P. Glycosylation of the self-recognizing Escherichia coli Ag43 autotransporter protein. J Bacteriol. 2006;188:1798-807 pubmed..However, the glycosylated form of Ag43 enhances bacterial binding to human cell lines, whereas the nonglycosylated version of Ag43 does not to confer this property. ..
- Hakansson K. The structure of Mg-ATPase nucleotide-binding domain at 1.6 A resolution reveals a unique ATP-binding motif. Acta Crystallogr D Biol Crystallogr. 2009;65:1181-6 pubmed publisher..This motif consists of a short polypeptide stretch running very close to the ATP-binding site, while in Ca-ATPase the binding site is more open, with the corresponding polypeptide segment folded away from the active site. ..
- S rensen I, Dandanell G. Identification and sequence analysis of Sulfolobus solfataricus purE and purK genes. FEMS Microbiol Lett. 1997;154:173-80 pubmed..coli. PurE seems to be more closely related to eubacteria than to other archaea and to eukaryotes. Also the purK gene, which has not yet been found in other archaea, is more closely related to eubacteria than to eukaryotes...
- Christensen S, Gerdes K. RelE toxins from bacteria and Archaea cleave mRNAs on translating ribosomes, which are rescued by tmRNA. Mol Microbiol. 2003;48:1389-400 pubmed..RelE proteins from Gram-positive Bacteria and Archaea cleaved tmRNA with a pattern similar to that of E. coli RelE, suggesting that the function and target of RelE may be conserved across the prokaryotic domains. ..
- Boston T, Atlung T. FNR-mediated oxygen-responsive regulation of the nrdDG operon of Escherichia coli. J Bacteriol. 2003;185:5310-3 pubmed..Transcription of the other two nucleotide reductase operons, nrdAB and nrdEF, did not respond to oxygen conditions in a wild-type background, but nrdAB expression was increased in the fnr mutant under anaerobic conditions. ..
- Karring H, Mathu S, van Duin J, Clark B, Kraal B, Knudsen C. Qbeta-phage resistance by deletion of the coiled-coil motif in elongation factor Ts. J Biol Chem. 2004;279:1878-84 pubmed..We propose that the role of EF-Ts in the Qbeta-polymerase complex is to control and trap EF-Tu in a stable conformation with affinity for RNA templates while unable to bind aminoacyl-tRNA. ..
- Long K, Poehlsgaard J, Kehrenberg C, Schwarz S, Vester B. The Cfr rRNA methyltransferase confers resistance to Phenicols, Lincosamides, Oxazolidinones, Pleuromutilins, and Streptogramin A antibiotics. Antimicrob Agents Chemother. 2006;50:2500-5 pubmed..In addition, the findings described in this study represent the first report of a gene conferring transferable resistance to pleuromutilins and oxazolidinones. ..
- Kristensen H, Valentin Hansen P, Søgaard Andersen L. CytR/cAMP-CRP nucleoprotein formation in E. coli: the CytR repressor binds its operator as a stable dimer in a ternary complex with cAMP-CRP. J Mol Biol. 1996;260:113-9 pubmed
- Laursen B, Mortensen K, Sperling Petersen H, Hoffman D. A conserved structural motif at the N terminus of bacterial translation initiation factor IF2. J Biol Chem. 2003;278:16320-8 pubmed..The results of the present work will provide guidance for the design of future experiments directed toward understanding the functional roles of this widely conserved structural domain within IF2. ..
- Klemm P, Hjerrild L, Gjermansen M, Schembri M. Structure-function analysis of the self-recognizing Antigen 43 autotransporter protein from Escherichia coli. Mol Microbiol. 2004;51:283-96 pubmed..Finally, we found that in spite of their different aggregative pattern all Ag43 variants promoted biofilm formation to abiotic surfaces. ..
- Pedersen H, Søgaard Andersen L, Holst B, Gerlach P, Bremer E, Valentin Hansen P. cAMP-CRP activator complex and the CytR repressor protein bind co-operatively to the cytRP promoter in Escherichia coli and CytR antagonizes the cAMP-CRP-induced DNA bend. J Mol Biol. 1992;227:396-406 pubmed..The case described here, in which a protein-induced DNA bend is modulated by a second protein, may illustrate a mechanism that applies to other regulatory systems. ..
- Seeger C, Poulsen C, Dandanell G. Identification and characterization of genes (xapA, xapB, and xapR) involved in xanthosine catabolism in Escherichia coli. J Bacteriol. 1995;177:5506-16 pubmed..In minicells, the XapB polypeptide was found primarily in the membrane fraction, indicating that XapB is a transport protein like NupG and is involved in the transport of xanthosine. ..
- Sørensen K, Hove Jensen B. Ribose catabolism of Escherichia coli: characterization of the rpiB gene encoding ribose phosphate isomerase B and of the rpiR gene, which is involved in regulation of rpiB expression. J Bacteriol. 1996;178:1003-11 pubmed
- Kilstrup M, Kristiansen K. Rapid genome walking: a simplified oligo-cassette mediated polymerase chain reaction using a single genome-specific primer. Nucleic Acids Res. 2000;28:E55 pubmed..Therefore, the presence of the vectorette mismatched region is not required when unphosphorylated cassettes are used. As an example we report the amplification of the era gene from Lactococcus lactis...
- Kruse T, Gerdes K. Bacterial DNA segregation by the actin-like MreB protein. Trends Cell Biol. 2005;15:343-5 pubmed..In particular, a recent study by Gitai et al. demonstrates that MreB specifically binds to and segregates the replication origin of the bacterial chromosome. ..
- Danielsen S, Kilstrup M, Barilla K, Jochimsen B, Neuhard J. Characterization of the Escherichia coli codBA operon encoding cytosine permease and cytosine deaminase. Mol Microbiol. 1992;6:1335-44 pubmed..A topological model for the cytosine permease in the cytoplasmic membrane is proposed. ..
- Jensen C, Brown S, Pedersen S. Effect of 4.5S RNA depletion on Escherichia coli protein synthesis and secretion. J Bacteriol. 1994;176:2502-6 pubmed..After further depletion of 4.5S RNA, accumulation of precursors of secreted proteins was observed under some growth conditions. ..
- Nørregaard Madsen M, McFall E, Valentin Hansen P. Organization and transcriptional regulation of the Escherichia coli K-12 D-serine tolerance locus. J Bacteriol. 1995;177:6456-61 pubmed..Additionally, the activity of the dsdXA promoter depends on the cyclic AMP receptor protein, and the two activators act in concert to synergistically activate transcription. ..
- Møllegaard N, Rasmussen P, Valentin Hansen P, Nielsen P. Characterization of promoter recognition complexes formed by CRP and CytR for repression and by CRP and RNA polymerase for activation of transcription on the Escherichia coli deoP2 promoter. J Biol Chem. 1993;268:17471-7 pubmed..Finally, the results show that CytR and RNA polymerase are rivals that compete for binding with CRP at deoP2 and that CytR functions as an antiactivator. ..
- Nørholm M, Dandanell G. Specificity and topology of the Escherichia coli xanthosine permease, a representative of the NHS subfamily of the major facilitator superfamily. J Bacteriol. 2001;183:4900-4 pubmed..A 12-transmembrane-segment model was confirmed by translational fusions to alkaline phosphatase and the alpha fragment of beta-galactosidase. ..
- Parmeggiani A, Krab I, Watanabe T, Nielsen R, Dahlberg C, Nyborg J, et al. Enacyloxin IIa pinpoints a binding pocket of elongation factor Tu for development of novel antibiotics. J Biol Chem. 2006;281:2893-900 pubmed..This work clarifies the structural background of the action of enacyloxin IIa and compares its properties with those of kirromycin, opening new perspectives for structure-guided design of novel antibiotics. ..
- Nygaard P, Smith J. Evidence for a novel glycinamide ribonucleotide transformylase in Escherichia coli. J Bacteriol. 1993;175:3591-7 pubmed..Under these conditions GAR accumulated. Addition of purine compounds or formate prevented growth inhibition. The regulation of the level of GAR transformylase T is controlled by the PurR protein and hypoxanthine. ..