Experts and Doctors on escherichia coli in South Korea

Summary

Locale: South Korea
Topic: escherichia coli

Top Publications

  1. Kim C, Han S, Kim K, Cho B, Kim Y, Koo B, et al. Cloning and expression of pyrroloquinoline quinone (PQQ) genes from a phosphate-solubilizing bacterium Enterobacter intermedium. Curr Microbiol. 2003;47:457-61 pubmed
    ..We speculate that production of PQQ in E. coli DH5alpha expressing the pqq cluster activates an endogenous glucose dehydrogenase to permit gluconic acid secretion that solubilizes the insoluble phosphate. ..
  2. Shin D, Lee J, Choy H, Hong Y. Generation and characterization of Clostridium septicum alpha toxin mutants and their use in diagnosing paroxysmal nocturnal hemoglobinuria. Biochem Biophys Res Commun. 2004;324:753-60 pubmed
    ..Thus, this modified bacterial toxin can be employed instead of costly monoclonal antibodies to diagnose PNH patients. ..
  3. Supangat S, Choi Y, Park Y, Son D, Han C, Lee K. Expression, purification, crystallization and preliminary X-ray analysis of sepiapterin reductase from Chlorobium tepidum. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005;61:202-4 pubmed
    ..The structure of CT-SR has been determined using MAD phasing. There is one CT-SR tetramer in the asymmetric unit formed by two closely interacting CT-SR dimers. The solvent content is calculated to be about 67.2%. ..
  4. Singh B, Lee C, Sohng J. Precursor for biosynthesis of sugar moiety of doxorubicin depends on rhamnose biosynthetic pathway in Streptomyces peucetius ATCC 27952. Appl Microbiol Biotechnol. 2010;85:1565-74 pubmed publisher
    ..Hence, rmbB provides TDP-4-keto-6-deoxy-D-glucose as a nucleotide sugar precursor for the biosynthesis of doxorubicin. ..
  5. Park S, Kim D, Kim D, Kim D, Jung H, Pan J, et al. Engineering bacterial cytochrome P450 (P450) BM3 into a prototype with human P450 enzyme activity using indigo formation. Drug Metab Dispos. 2010;38:732-9 pubmed publisher
  6. Nguyen T, Ryu H, Lee S, Hwang S, Breton V, Rhee J, et al. Virtual screening identification of novel severe acute respiratory syndrome 3C-like protease inhibitors and in vitro confirmation. Bioorg Med Chem Lett. 2011;21:3088-91 pubmed publisher
    ..11±1.6 and 9.93±0.44 ?M. Hydrophobic and hydrogen bond interactions of compound with amino acid residues in the active site of 3CL(pro) were also identified. ..
  7. Park S, Lee J, Chang W, Choy H, Kim G. A coupling process for improving purity of bacterial minicells by holin/lysin. J Microbiol Methods. 2011;86:108-10 pubmed publisher
    ..A two-step protocol coupling an autolysin (holin/lysin) step with a conventional centrifugation step achieved a purity of the minicells similar to that resulting from successive six- or seven-step methods. ..
  8. Park J, Sun J, Park S, Hwang H, Park M, Shin M, et al. Crystal structure of Cmr5 from Pyrococcus furiosus and its functional implications. FEBS Lett. 2013;587:562-8 pubmed publisher
    ..In vitro assays indicate that pfCmr5 interacts with pfCmr4. These structural and biophysical data might help in understanding the complicated and ill-characterized Cmr effector complex...
  9. Kang G, Song H, Song D, Kim M, Rho S, Kim D, et al. Overexpression and purification of the RyR1 pore-forming region. Protein Pept Lett. 2007;14:742-6 pubmed
    ..The protein was then purified using nickel-affinity chromatography and gel filtration. Gel filtration analysis confirmed that the RyR1 fragment containing the pore-forming region (amino acids 4829-5037) is sufficient to form a tetramer. ..

More Information

Publications113 found, 100 shown here

  1. Baek S, Kim S, Ha S. Reduction of Escherichia coli on surfaces of utensils and development of a predictive model as a function of concentration and exposure time of chlorine. Foodborne Pathog Dis. 2012;9:1-6 pubmed publisher
    ..It has measured the R(2) value to 0.9746. Therefore, the models presented in this study could be used to determine the minimum concentrations of chlorine and exposure times needed to control E. coli on the surfaces of various materials. ..
  2. Park S, Byeon Y, Back K. Functional analyses of three ASMT gene family members in rice plants. J Pineal Res. 2013;55:409-15 pubmed publisher
    ..ASMT1 and ASMT2 transcripts were highly expressed in stems and flowers, but ASMT3 was barely detectable in any of the plant organs. All three ASMT mRNAs were simultaneously induced in treatments with abscisic and methyl jasmonic acids. ..
  3. Kim M, Yoon H, Park S, Kim D, Pyo A, Kim H, et al. Engineering of monobody conjugates for human EphA2-specific optical imaging. PLoS ONE. 2017;12:e0180786 pubmed publisher
    ..E1-Rluc8 signals were detected at 4 h, peaked at 12 h, and were undetectable at 24 h. These results suggest the potential of E1-Rluc8 as an EphA2-specific optical imaging agent. ..
  4. Kim S, Lee P. Functional expression and extension of staphylococcal staphyloxanthin biosynthetic pathway in Escherichia coli. J Biol Chem. 2012;287:21575-83 pubmed publisher
    ..coli, staphyloxanthin-like compounds, which contained altered fatty acid acyl chains, and novel carotenoid compounds were produced, indicating functional expression and coordination of the six staphyloxanthin pathway enzymes. ..
  5. Kim Y, Jung S, Kim K, Bae H. Role of pCeMT, a putative metallothionein from Colocasia esculenta, in response to metal stress. Plant Physiol Biochem. 2013;64:25-32 pubmed publisher
  6. Kang H, Lee J, Kim D, Day D, Robyt J, Park K, et al. Cloning and expression of Lipomyces starkeyi alpha-amylase in Escherichia coli and determination of some of its properties. FEMS Microbiol Lett. 2004;233:53-64 pubmed
    ..LSA did not hydrolyze soluble starch in the present of 2% (w/v) of acarbose. Kinetics of LSA was carried out by using starch as a substrate and the inhibition type of acarbose was the mixed non-competitive type (ki = 3.4 microM). ..
  7. Lee D, Kang Y, Park M, Lee Y, Seo H, Kim T, et al. Effects of 13-alkyl-substituted berberine alkaloids on the expression of COX-II, TNF-alpha, iNOS, and IL-12 production in LPS-stimulated macrophages. Life Sci. 2003;73:1401-12 pubmed
  8. Kim Y, Seo H, Min J, Kim Y, Ban Y, Han K, et al. Enhanced degradation and toxicity reduction of dihexyl phthalate by Fusarium oxysporum f. sp. pisi cutinase. J Appl Microbiol. 2007;102:221-8 pubmed
    ..This study describes the enhanced degradation and detoxification of DHP using Fusarium oxysporum f. sp. pisi cutinase. ..
  9. Kim J, Kim W, Kwak K, Oh S, Han Y, Kang H. Zinc finger-containing glycine-rich RNA-binding protein in Oryza sativa has an RNA chaperone activity under cold stress conditions. Plant Cell Environ. 2010;33:759-68 pubmed publisher
  10. Lim Y, Cha M, Kim H, Kim I. The thiol-specific antioxidant protein from human brain: gene cloning and analysis of conserved cysteine regions. Gene. 1994;140:279-84 pubmed
  11. Kim Y, Patel D, Lee D, Song Y, Bae H. High cadmium-binding ability of a novel Colocasia esculenta metallothionein increases cadmium tolerance in Escherichia coli and tobacco. Biosci Biotechnol Biochem. 2011;75:1912-20 pubmed
    ..The presence of an extra CXC motif in CeMT2b contributed to the enhanced Cd-tolerance. The present study provides the first insight into the ability of type-2 plant MT to bind physiological Cd. ..
  12. Kim H, Phan T, Jang T, Koh M, Kim S. Characterization of Methylophaga sp. strain SK1 cytochrome cL expressed in Escherichia coli. J Microbiol. 2005;43:499-502 pubmed
    ..These results suggest that recombinant cytochrome c(L) acts as a physiological primary electron acceptor for MDH. ..
  13. Kim S, Jeong D, Chi S, Lee J, Ryu S. Crystal structure of proteolytic fragments of the redox-sensitive Hsp33 with constitutive chaperone activity. Nat Struct Biol. 2001;8:459-66 pubmed
  14. Kim T, Lee J. Cloning and expression of genes encoding transferrin-binding protein A and B from Actinobacillus pleuropneumoniae serotype 5. Protein Expr Purif. 2006;45:235-40 pubmed
    ..This result indicates that the recombinant proteins can induce immunological responses and might be useful as candidate targets for A. pleuropneumoniae vaccination. ..
  15. Unno T, Han D, Jang J, Lee S, Ko G, Choi H, et al. Absence of Escherichia coli phylogenetic group B2 strains in humans and domesticated animals from Jeonnam Province, Republic of Korea. Appl Environ Microbiol. 2009;75:5659-66 pubmed publisher
    ..Results of this study also suggest that the relationship between the presence and types of virulence genes and phylogenetic groupings may differ among geographically distinct E. coli populations. ..
  16. Jiang S, Phan T, Nam T, Nguyen V, Kim H, Bom H, et al. Inhibition of tumor growth and metastasis by a combination of Escherichia coli-mediated cytolytic therapy and radiotherapy. Mol Ther. 2010;18:635-42 pubmed publisher
    ..The results described here indicate that therapy with engineered E. coli could significantly improve the results of RT, and could exert a striking inhibitory effect on the development of lung metastasis. ..
  17. Kim W, Kim J, Jung H, Oh S, Han Y, Kang H. Comparative analysis of Arabidopsis zinc finger-containing glycine-rich RNA-binding proteins during cold adaptation. Plant Physiol Biochem. 2010;48:866-72 pubmed publisher
    ..coli. ..
  18. Pandey R, Malla S, Simkhada D, Kim B, Sohng J. Production of 3-O-xylosyl quercetin in Escherichia coli. Appl Microbiol Biotechnol. 2013;97:1889-901 pubmed publisher
    ..Bioconversion was further improved by 98 % when the reaction was scaled up in a 3-L fermentor at 36 h. ..
  19. Lee B, Lee H, Jung Y, Kim S, Lee Y, Chang S, et al. The effects of a novel botanical agent on lipopolysaccharide-induced alveolar bone loss in rats. J Periodontol. 2013;84:1221-9 pubmed publisher
    ..The botanical mixture showed a potential adjunctive effect in the treatment of periodontitis. However, the present findings are obtained in vitro and in a rat model, so further clinical study is needed for its clinical application. ..
  20. Im Y, Na Y, Kang G, Rho S, Kim M, Lee J, et al. The active site of a lon protease from Methanococcus jannaschii distinctly differs from the canonical catalytic Dyad of Lon proteases. J Biol Chem. 2004;279:53451-7 pubmed publisher
  21. Dhakal B, Lee W, Kim Y, Choy H, Ahnn J, Rhee J. Caenorhabditis elegans as a simple model host for Vibrio vulnificus infection. Biochem Biophys Res Commun. 2006;346:751-7 pubmed
    ..vulnificus infections also play important roles in the killing of C. elegans, and thereby suggest that C. elegans is a favorable model for host-parasite interaction. ..
  22. Yim S, Kim D, Jung H, Pan J, Kang H, Ahn T, et al. Surface display of heme- and diflavin-containing cytochrome P450 BM3 in Escherichia coli: a whole cell biocatalyst for oxidation. J Microbiol Biotechnol. 2010;20:712-7 pubmed
  23. Song S, Park C. Utilization of D-ribose through D-xylose transporter. FEMS Microbiol Lett. 1998;163:255-61 pubmed
    ..Ribose uptake was also reduced by the secondary mutations. An overexpression of xylFGH under Ptrc promoter supported enhanced growth on ribose. These results indicate that D-ribose can be transported through the XylFGH transporter...
  24. Lee S, Hwang Y, Kim Y, Kwon K, Kim H, Kim K, et al. Cyclophilin a binds to peroxiredoxins and activates its peroxidase activity. J Biol Chem. 2001;276:29826-32 pubmed
    ..In addition, Cys(115) and Cys(161) of hCyP-A were found to be involved in the activation and the reduction of Prx. ..
  25. Jeong S, Bae I, Lee J, Sohn S, Kang G, Jeon G, et al. Molecular characterization of extended-spectrum beta-lactamases produced by clinical isolates of Klebsiella pneumoniae and Escherichia coli from a Korean nationwide survey. J Clin Microbiol. 2004;42:2902-6 pubmed publisher
    ..Using MIC patterns and DNA sequencing analysis, we postulated a possible evolution scheme among TEM-type beta-lactamases in Korea: from TEM-1 to TEM-19, from TEM-19 to TEM-20, and from TEM-20 to TEM-52...
  26. Kim J, Kim K, Oh T, Park C, Kang H. Functional characterization of DEAD-box RNA helicases in Arabidopsis thaliana under abiotic stress conditions. Plant Cell Physiol. 2008;49:1563-71 pubmed publisher
  27. Park K, Choi S, Ko M, Park C. Novel sigmaF-dependent genes of Escherichia coli found using a specified promoter consensus. FEMS Microbiol Lett. 2001;202:243-50 pubmed
    ..An overproduction or inactivation of these genes did not exhibit any notable phenotypes in motility or chemotaxis. ..
  28. Mitchell R, Gu M. Construction and evaluation of nagR-nagAa::lux fusion strains in biosensing for salicylic acid derivatives. Appl Biochem Biotechnol. 2005;120:183-98 pubmed
    ..Within this strain, NAGK-1768, the supplemental activity of the NahR protein on the nagAa promoter, was shown to extend both the range of chemicals detected and the sensitivity. ..
  29. Xu T, Han J, Kang H. Structural features important for the RNA chaperone activity of zinc finger-containing glycine-rich RNA-binding proteins from wheat (Triticum avestivum) and rice (Oryza sativa). Phytochemistry. 2013;94:28-35 pubmed publisher
    ..Collectively, these results indicate that a specific modular arrangement of RRM domain and the disordered C-terminal region determines the RNA chaperone activity of RZs in cells. ..
  30. Gu M, Chang S. Soil biosensor for the detection of PAH toxicity using an immobilized recombinant bacterium and a biosurfactant. Biosens Bioelectron. 2001;16:667-74 pubmed
    ..This biosensor system using a biosurfactant may be applied as an in-situ biosensor to detect the toxicity of hydrophobic contaminants in soils and for performance evaluation of PAH degradation in soils. ..
  31. Kim S, Moon D, Lee C, Nam S, Kim P. Comparison of the effects of NADH- and NADPH-perturbation stresses on the growth of Escherichia coli. Curr Microbiol. 2009;58:159-63 pubmed publisher
    ..8 g glucose/g cell, while that of control and NADPH-perturbed cells was very similar (3.6 vs 3.8 g glucose/g cell) during the perturbation phase. Therefore, NADH perturbation had a larger effect than NADPH on cellular growth. ..
  32. Kwak K, Kang H, Han K, Ahn S. Molecular cloning, characterization, and stress-responsive expression of genes encoding glycine-rich RNA-binding proteins in Camelina sativa L. Plant Physiol Biochem. 2013;68:44-51 pubmed publisher
    ..Collectively, these results suggest that the stress-responsive CsGRP2s play a role as an RNA chaperone during the stress adaptation process in camelina. ..
  33. Park J, Cho Y, Kwon I, Jeong S, Bae Y. Assessment of PEO/PTMO multiblock copolymer/segmented polyurethane blends as coating materials for urinary catheters: in vitro bacterial adhesion and encrustation behavior. Biomaterials. 2002;23:3991-4000 pubmed
  34. Shin M, Song M, Rhee J, Hong Y, Kim Y, Seok Y, et al. DNA looping-mediated repression by histone-like protein H-NS: specific requirement of Esigma70 as a cofactor for looping. Genes Dev. 2005;19:2388-98 pubmed
    ..Expression of this class of genes by Esigma38 in the stationary phase is not due to its promoter specificity but to the architecture of the promoter . Esigma38 complex. ..
  35. Le U, Kim H, Kwon J, Kim M, Nguyen V, Jiang S, et al. Engineering and visualization of bacteria for targeting infarcted myocardium. Mol Ther. 2011;19:951-9 pubmed publisher
    ..This current proof-of-principle study demonstrates for the first time the capacity of Salmonellae to target infarcted myocardium and to serve as a vehicle for the selective delivery of therapeutic agents in MI. ..
  36. Kim K, Jeong J, Lim D, Hong Y, Yun M, Min J, et al. A novel balanced-lethal host-vector system based on glmS. PLoS ONE. 2013;8:e60511 pubmed publisher
  37. Lee K, Oh J. Design and synthesis of novel antimicrobial pseudopeptides with selective membrane-perturbation activity. Bioorg Med Chem. 2000;8:833-9 pubmed
    ..The present result suggest that backbone modifications can be a great tool for developing pseudopeptides with improved biological activity and bioavailability from cytolytic peptides. ..
  38. Kim M, Han K, Kang H, Rhee S, Seo J. Coexpression of BiP increased antithrombotic hirudin production in recombinant Saccharomyces cerevisiae. J Biotechnol. 2003;101:81-7 pubmed
    ..The mean size of the recombinant yeast cells expressing the BiP gene remained at a relatively constant level compared with the control strains of which size increased after the onset of hirudin expression by the GAL10 promoter. ..
  39. Mitchell R, Gu M. An Escherichia coli biosensor capable of detecting both genotoxic and oxidative damage. Appl Microbiol Biotechnol. 2004;64:46-52 pubmed
    ..Finally, several chemical mixtures that cause various stress responses were tested to demonstrate the ability of this biosensor to detect specific stress responses within a multiple toxicity environment. ..
  40. Lee P, Holtzapple E, Schmidt Dannert C. Novel activity of Rhodobacter sphaeroides spheroidene monooxygenase CrtA expressed in Escherichia coli. Appl Environ Microbiol. 2010;76:7328-31 pubmed publisher
    ..The heme-containing CrtA is not a P450 enzyme but a new type of oxygenase...
  41. Kim J, Kang S, Woo J, Lee J, Jeong B, Kim S. Screening and its potential application of lipolytic activity from a marine environment: characterization of a novel esterase from Yarrowia lipolytica CL180. Appl Microbiol Biotechnol. 2007;74:820-8 pubmed
    ..This study represents a novel esterase of type B1 carboxylesterase/lipase family from a marine isolate, showing a potential usage as a biocatalyst because of enantioselectivity toward racemic ofloxacin ester. ..
  42. Nho S, Ha M, Kim K, Kim T, Jang H, Cha I, et al. Complete genome sequence of the bacteriophages ECBP1 and ECBP2 isolated from two different Escherichia coli strains. J Virol. 2012;86:12439-40 pubmed publisher
    ..coli strains which might be used as therapeutic agents in combination with alternative antibiotics. ..
  43. Yoo S, Shim Y, Seong I, Seol J, Kang M, Chung C. Mutagenesis of two N-terminal Thr and five Ser residues in HslV, the proteolytic component of the ATP-dependent HslVU protease. FEBS Lett. 1997;412:57-60 pubmed
    ..The mutational studies also revealed that both Thr, Ser103, and Ser172, but not Ser124, are involved in the interaction of HslV with HslU and hence in the activation of HslU ATPase as well as in the HslVU complex formation. ..
  44. Ahn T, Ko J, Cho E, Yun C. Conformational change of Escherichia coli signal recognition particle Ffh is affected by the functionality of signal peptides of ribose-binding protein. Mol Cells. 2009;27:681-7 pubmed publisher
    ..However, MT had marginal effect on these conformational changes suggesting that the in vivo functionality of signal peptide is important in the interaction with Ffh and concomitant structural change of the protein. ..
  45. Quan L, Min J, Yang D, Kim Y, Yang D. Enzymatic biotransformation of ginsenoside Rb1 to 20(S)-Rg3 by recombinant ?-glucosidase from Microbacterium esteraromaticum. Appl Microbiol Biotechnol. 2012;94:377-84 pubmed publisher
    ..Bgp1 hydrolyzed the ginsenoside Rb1 along the following pathway: Rb1???Rd???20(S)-Rg3. This is the first report of the biotransformation of ginsenoside Rb1 to ginsenoside 20(S)-Rg3 using the recombinant ?-glucosidase. ..
  46. Song W, Kim Y, Sim S, Hwang S, Lee J, Lee Y, et al. Antibiotic stress-induced modulation of the endoribonucleolytic activity of RNase III and RNase G confers resistance to aminoglycoside antibiotics in Escherichia coli. Nucleic Acids Res. 2014;42:4669-81 pubmed publisher
    ..coli and related gram-negative bacteria to aminoglycoside antibiotics. ..
  47. Rani S, Park C, Sreenivasaiah P, Kim D. Characterization of Ca(2+)-Dependent Protein-Protein Interactions within the Ca(2+) Release Units of Cardiac Sarcoplasmic Reticulum. Mol Cells. 2016;39:149-55 pubmed publisher
    ..Among them, only CSQ2 or RyR2 competitively inhibited TRN-HRC binding, suggesting that HRC can confer functional refractoriness to CRU, which could be beneficial for reloading of Ca(2+) into SR at intermediate Ca(2+) concentrations. ..
  48. Choi W, Yamaguchi Y, Lee J, Jang K, Inouye M, Kim S, et al. Translation-dependent mRNA cleavage by YhaV in Escherichia coli. FEBS Lett. 2017;591:1853-1861 pubmed publisher
    ..From these results, we conclude that YhaV is a ribosome-dependent toxin that cleaves mRNA in a translation-dependent manner. ..
  49. Shin S, Song S, Lee D, Pan J, Park C. Involvement of iclR and rpoS in the induction of acs, the gene for acetyl coenzyme A synthetase of Escherichia coli K-12. FEMS Microbiol Lett. 1997;146:103-8 pubmed
    ..Mutations in iclR for the repressor of the glyoxylate shunt and in rpoS for the stationary phase sigma factor reduced the consumption of acetate mediated by Acs, indicating that both are involved in acs regulation. ..
  50. Jang Y, Chung K, Park C, Yoo H. Fission yeast dihydrolipoamide dehydrogenase gene is involved in G1/S cell cycle progression. Biochim Biophys Acta. 1997;1358:229-39 pubmed
    ..However, it is certain that malfunction of this Dld1 protein blocks the progression of cell cycle from G1 to S phase. The sequence of the dld1+ gene is available in EMBL/GenBank under Accession Number L40360. ..
  51. Kim S, Cha I, Kim K, Kim Y, Lee Y. Cloning and sequence analysis of another Shiga-like toxin IIe variant gene (slt-IIera) from an Escherichia coli R107 strain isolated from rabbit. Microbiol Immunol. 1997;41:805-8 pubmed
    ..Recombinant SLT-IIera and SLT-IIe produced using an E. coli host-vector system showed similar cytotoxicity, suggesting that the variations in the structural gene of SLT-IIera have no significant effect on cytotoxic level. ..
  52. Seong C, Kim Y, Chung H, Park D, Yim J, Baek K, et al. Isolation and characterization of the Drosophila melanogaster cDNA encoding the sepiapterin reductase. Biochim Biophys Acta. 1998;1443:239-44 pubmed
    ..Southern analysis suggests that the Drosophila SR gene is encoded by a single copy gene. RNA blot analysis revealed that the gene expresses 1.5 kb mRNA in both adult heads and bodies. ..
  53. Seo J, Song K, Jang K, Kim C, Jung B, Rhee S. Molecular cloning of a gene encoding the thermoactive levansucrase from Rrahnella aquatilis and its growth phase-dependent expression in Eescherichia coli. J Biotechnol. 2000;81:63-72 pubmed
  54. Suh H, Lee H, Jung J. Mycosporine glycine protects biological systems against photodynamic damage by quenching singlet oxygen with a high efficiency. Photochem Photobiol. 2003;78:109-13 pubmed
    ..The results suggest that MG probably together with some other active MAA may play an important role in protecting marine organisms against sunlight damage by eliminating 1O2 generated from certain endogenous photosensitizers. ..
  55. Kim M, Im Y, Lee J, Eom S. Crystal structure of quinolinic acid phosphoribosyltransferase from Helicobacter pylori. Proteins. 2006;63:252-5 pubmed publisher
  56. Choi S, Matsuda S, Hoshino T, Peng X, Misawa N. Characterization of bacterial beta-carotene 3,3'-hydroxylases, CrtZ, and P450 in astaxanthin biosynthetic pathway and adonirubin production by gene combination in Escherichia coli. Appl Microbiol Biotechnol. 2006;72:1238-46 pubmed
    ..The present results suggest that the Brevundimonas sp. SD212 crtZ and T. thermophilus HB27 CYP175A1 genes could, respectively, be used for the efficient production of astaxanthin and adonirubin in heterologous hosts...
  57. Kim M, Cho M, Song H, Kim D, Park B, Lee J, et al. Crystal structure of UDP-N-acetylenolpyruvylglucosamine reductase (MurB) from Thermus caldophilus. Proteins. 2007;66:751-4 pubmed
  58. Seo J, Kang S, Won D, Kim M, Ryu J, Kang S, et al. Absolute configuration-dependent epoxide formation from isoflavan-4-ol stereoisomers by biphenyl dioxygenase of Pseudomonas pseudoalcaligenes strain KF707. Appl Microbiol Biotechnol. 2011;89:1773-82 pubmed publisher
  59. Park H, Park J, Choi J, Lee J, Kim B, Jung C, et al. Structures of the ?-class carbonic anhydrase homologue YrdA suggest a possible allosteric switch. Acta Crystallogr D Biol Crystallogr. 2012;68:920-6 pubmed publisher
    ..Even though significant CA activity could not be detected using purified ecYrdA, these structural features have potential functional implications for ?-CA-related proteins. ..
  60. Han D, Kurusarttra S, Ryu J, Kanaly R, Hur H. Production of natural fragrance aromatic acids by coexpression of trans-anethole oxygenase and p-anisaldehyde dehydrogenase genes of Pseudomonas putida JYR-1 in Escherichia coli. J Agric Food Chem. 2012;60:11972-9 pubmed publisher
    ..Because of the relaxed substrate specificity and high transformation rates by coexpressed TAO and PAADH in E. coli , the engineered strain has potential to be applied in the fragrance industry...
  61. Lee J, Kim M, Seo Y, Lee Y, Park H, Byun S, et al. The catalytic activity of a recombinant single chain variable fragment nucleic acid-hydrolysing antibody varies with fusion tag and expression host. Arch Biochem Biophys. 2017;633:110-117 pubmed publisher
    ..Thus, we demonstrate that the antigen-binding and catalytic activities of a catalytic antibody can be separately affected by the presence of additional residues at the N- and C-termini, and by the host cell type. ..
  62. Oh T, Kim I. Identification of genetic factors altering the SOS induction of DNA damage-inducible yebG gene in Escherichia coli. FEMS Microbiol Lett. 1999;177:271-7 pubmed
    ..Our results demonstrated that the induction of a DNA damage-inducible yebG gene of E. coli is dependent on cyclic AMP and H-NS. ..
  63. Kim I, Oh T. 2-Mercaptoethylamine, radioprotector, inhibits the induction of the oxidative stress-inducible (soi) gene by paraquat in Escherichia coli. Pharmacol Res. 2000;42:429-33 pubmed
    ..At concentrations above 1 mm, MEA effectively inhibited the NBT reduction in a concentration-dependent fashion. Our results demonstrated that MEA has an ability to scavenge.O(2)(-), and so protects against.O(2)(-)-mediated damage...
  64. Kim M, Song H, Kim Y, Rho S, Im Y, Lee J, et al. Crystallization and preliminary X-ray crystallographic analysis of orotate phosphoribosyltransferase from Helicobacter pylori. Mol Cells. 2003;15:361-3 pubmed
    ..8, b = 104.9, c = 281.1 A, alpha = beta = gamma = 90 degrees. A set of diffraction data was collected to 3.29 A resolution using synchrotron X-ray radiation. ..
  65. Lee J, Im Y, Rho S, Park S, Kim M, Cho S, et al. Crystallization and preliminary X-ray analysis of class II fructose-1,6-bisphosphate aldolase from Thermus caldophilus. Protein Pept Lett. 2003;10:511-5 pubmed
    ..9, b = 113.1, c = 115.7 A), with four molecules in the asymmetric unit. A complete diffraction data set was obtained from orthorhombic crystals at resolution of 2.2 A. ..
  66. Cho H, Huang L, Hamza A, Gao D, Zhan C, Tai H. Role of glutamine 148 of human 15-hydroxyprostaglandin dehydrogenase in catalytic oxidation of prostaglandin E2. Bioorg Med Chem. 2006;14:6486-91 pubmed
    ..This indicates that the side-chain oxygen or nitrogen atom at position 148 of 15-PGDH plays an important role in anchoring C-15 hydroxyl group of PGE2 through hydrogen bonding for catalytic reaction. ..
  67. Chang J, Yoon I, Kim K. Isolation and ars detoxification of arsenite-oxidizing bacteria from abandoned arsenic-contaminated mines. J Microbiol Biotechnol. 2007;17:812-21 pubmed
    ..Accordingly, the present results suggest that arsR, arsD, arsAB, arsA, arsB, arsC, arsH, arrA, arrB, aoxA, aoxB, aoxC, aoxD, aroA, and aroB may be useful for arsenite-oxidizing bacteria in abandoned arsenic-contaminated mines...
  68. Kim D, Kim S, Choi K, Lee J, Kim E. Molecular cloning and functional characterization of the genes encoding benzoate and p-hydroxybenzoate degradation by the halophilic Chromohalobacter sp. strain HS-2. FEMS Microbiol Lett. 2008;280:235-41 pubmed publisher
    ..When expressed in Escherichia coli, benzoate 1,2-dioxygenase (BenABC) and p-hydroxybenzoate hydroxylase (PobA) transformed benzoate and p-hydroxybenzoate into cis-benzoate dihydrodiol and protocatechuate, respectively...
  69. Ahn T, Yun C, Chae H, Kim H, Chae H. Refolding and reconstitution of human recombinant Bax inhibitor-1 into liposomes from inclusion bodies expressed in Escherichia coli. Protein Expr Purif. 2009;66:35-8 pubmed publisher
    ..These conformational changes may be responsible for the proton ion-induced Ca(2+) release of BI-1. ..
  70. Ghimire G, Lee H, Sohng J. Improved squalene production via modulation of the methylerythritol 4-phosphate pathway and heterologous expression of genes from Streptomyces peucetius ATCC 27952 in Escherichia coli. Appl Environ Microbiol. 2009;75:7291-3 pubmed publisher
    ..1 mg/liter of squalene. This level was elevated to 11.8 mg/liter when there was also increased expression of dxs and idi, E. coli genes encoding 1-deoxy-d-xylulose 5-phosphate synthase and isopentenyl diphosphate isomerase. ..
  71. Kim M, Lee H. Growth-inhibiting activities of phenethyl isothiocyanate and its derivatives against intestinal bacteria. J Food Sci. 2009;74:M467-71 pubmed publisher
    ..In conclusion, aromatic isothiocyanates containing phenethyl-, benzyl-, and benzoyl-groups might be useful in the development of novel preventive and therapeutic agents against diseases caused by harmful intestinal bacteria...
  72. Jang T, Zheng C, Wu H, Jeon J, Park H. In vitro reconstitution of the interactions in the PIDDosome. Apoptosis. 2010;15:1444-52 pubmed publisher
    ..Instead, full-length RAIDD forms both binary and ternary complexes under a low salt condition. Successful in vitro reconstitution of the ternary complex provides a basis for further structural studies of the PIDDosome. ..
  73. Yu Y, Pandeya D, Liu M, Liu M, Hong S. Expression and purification of a functional human hepatitis B virus polymerase. World J Gastroenterol. 2010;16:5752-8 pubmed
    ..Ability of this purification approach to obtain human HBV-Pol in an enzymatically active form should be helpful for development of drugs for treatment of chronic hepatitis B. ..
  74. Choi Y, Yun J, Kim C, Choi S, Chai J, Jung U. Sinus augmentation using absorbable collagen sponge loaded with Escherichia coli-expressed recombinant human bone morphogenetic protein 2 in a standardized rabbit sinus model: a radiographic and histologic analysis. Clin Oral Implants Res. 2012;23:682-689 pubmed publisher
    ..However, the structural durability of ACS was not sufficient to maintain the augmented volume in the sinus. ..
  75. Joo S, Chung H, Raetz C, Garrett T. Activity and crystal structure of Arabidopsis thaliana UDP-N-acetylglucosamine acyltransferase. Biochemistry. 2012;51:4322-30 pubmed publisher
    ..This work shows that AtLpxA is a functional UDP-GlcNAc acyltransferase that is able to catalyze the same reaction as EcLpxA and supports the hypothesis that lipid A molecules are biosynthesized in Arabidopsis and other plants. ..
  76. Kim S, Kim M, Lee B, Lee P. Generation of structurally novel short carotenoids and study of their biological activity. Sci Rep. 2016;6:21987 pubmed publisher
    ..Our results demonstrate that a series of structurally novel carotenoids possessing biologically beneficial properties can be synthesized in E. coli. ..
  77. Lim C, Hwang W, Park E, Fuchs J. Cyclic AMP-dependent expression of the Escherichia coli serC-aroA operon. Biochim Biophys Acta. 1994;1218:250-3 pubmed
    ..These findings unambiguously indicate that the expression of the serC-aroA operon is positively controlled by cAMP. ..
  78. Shin D, Yoo S, Shim Y, Seol J, Kang M, Chung C. Mutational analysis of the ATP-binding site in HslU, the ATPase component of HslVU protease in Escherichia coli. FEBS Lett. 1996;398:151-4 pubmed
    ..These results suggest that the mutation in the ATP-binding site results in prevention of the binding of the adenine nucleotides to HslU and hence in impairment of both oligomerization and ATPase function of HslU. ..
  79. Seong C, Jeong S, Park D, Yoon J, Oh Y, Yim J, et al. Molecular characterization of the Drosophila melanogaster gene encoding the pterin 4alpha-carbinolamine dehydratase. Biochim Biophys Acta. 1998;1388:273-8 pubmed
    ..The potential promoter region, deduced from the determination of the transcription start point (tsp), lacks the distinct TATAAA box consensus sequence. ..
  80. Hyun C, Kim S, Park K, Suh J. Valine dehydrogenase from Streptomyces albus: gene cloning, heterologous expression and identification of active site by site-directed mutagenesis. FEMS Microbiol Lett. 2000;182:29-34 pubmed
    ..Kinetic analyses of the Lys-79 and Lys-91-mutated enzymes revealed that they are involved in the substrate binding site and catalysis, respectively, analogous to the corresponding residues in the homologous Ldh and Pdh. ..
  81. Back K, Jang S, Lee B, Schmidt A, Strack D, Kim K. Cloning and characterization of a hydroxycinnamoyl-CoA:tyramine N-(hydroxycinnamoyl)transferase induced in response to UV-C and wounding from Capsicum annuum. Plant Cell Physiol. 2001;42:475-81 pubmed
    ..THT expression was measured in different plant tissues, and was constitutive at a similar level in leaf, root, stem, flower and fruit. Induction of THT mRNA was correlated with an increase in THT protein...
  82. Kim W, Rhee H, Park B, Suk K, Cha S. Isolation of peptide ligands that inhibit glutamate racemase activity from a random phage display library. J Biomol Screen. 2000;5:435-40 pubmed
  83. Baek H, Suk K, Kim Y, Cha S. An improved helper phage system for efficient isolation of specific antibody molecules in phage display. Nucleic Acids Res. 2002;30:e18 pubmed
  84. Kwak J, Jiang H, Kendrick K. Transformation using in vivo and in vitro methylation in Streptomyces griseus. FEMS Microbiol Lett. 2002;209:243-8 pubmed
    ..griseus. We identified several restriction-modification systems that decrease the transformation efficiency. This research also led us to understand methylation profiles and restriction-modification systems in S. griseus...
  85. Sung D, Kang H. Prokaryotic and eukaryotic translational machineries respond differently to the frameshifting RNA signal from plant or animal virus. Virus Res. 2003;92:165-70 pubmed
    ..These results supports the hypothesis that frameshifting occurs by a specific interaction between ribosome and frameshifting signal. ..
  86. Lee H, Bahn S, Kang Y, Lee K, Kim H, Noh E, et al. Secretory low molecular weight phospholipase A2 plays important roles in cell elongation and shoot gravitropism in Arabidopsis. Plant Cell. 2003;15:1990-2002 pubmed
    ..These results suggest that AtsPLA(2)beta regulates the process of cell elongation and plays important roles in shoot gravitropism by mediating auxin-induced cell elongation. ..
  87. Im Y, Lee J, Park S, Park S, Rho S, Kang G, et al. Crystal structure of the Shank PDZ-ligand complex reveals a class I PDZ interaction and a novel PDZ-PDZ dimerization. J Biol Chem. 2003;278:48099-104 pubmed
    ..This configuration may provide a means of facilitating dimeric organization of PDZ-target assemblies. ..
  88. Park S, Kim C, Jeong W, Lee J, Seo S, Han Y, et al. Effects of two hemolymph proteins on humoral defense reactions in the wax moth, Galleria mellonella. Dev Comp Immunol. 2005;29:43-51 pubmed
    ..Of note, the Gm protein-24 was a significantly stronger activator of PPO cascade than apoLp-III. ..
  89. Ryu K, Kim J, Cho S, Park D, Park C, Cheong H, et al. Structural insights into the monosaccharide specificity of Escherichia coli rhamnose mutarotase. J Mol Biol. 2005;349:153-62 pubmed
    ..coli strain that carries a yiiL deletion. The presence of the yiiL gene is critical for efficient cell growth only when concentrations of l-rhamnose are limited. ..
  90. Kim Y, Kim J, Kang H. Cold-inducible zinc finger-containing glycine-rich RNA-binding protein contributes to the enhancement of freezing tolerance in Arabidopsis thaliana. Plant J. 2005;42:890-900 pubmed
    ..These results provide evidence that atRZ-1a affects seed germination and seedling growth under low temperature and plays a role in the enhancement of freezing tolerance in Arabidopsis plants. ..
  91. Park S, Kwak K, Oh T, Kim Y, Kang H. Cold shock domain proteins affect seed germination and growth of Arabidopsis thaliana under abiotic stress conditions. Plant Cell Physiol. 2009;50:869-78 pubmed publisher
  92. Choi B, Jeong S, Wang G, Kim H, Kim B, Hwang H, et al. Temporal induction of secretory leukocyte protease inhibitor (SLPI) in odontoblasts by lipopolysaccharide and wound infection. J Endod. 2009;35:997-1002 pubmed publisher
    ..We report for the first time that SLPI is expressed temporally in infected odontoblasts and may participate in the anti-inflammatory response through NF-kappaB signaling in odontoblast-like cells. ..