Experts and Doctors on escherichia coli in Japan

Summary

Locale: Japan
Topic: escherichia coli

Top Publications

  1. Ogawa T, Okazaki T. Concurrent transcription from the gid and mioC promoters activates replication of an Escherichia coli minichromosome. Mol Gen Genet. 1991;230:193-200 pubmed
    ..These two promoters are suggested to be under stringent control. ..
  2. Hanada H, Hasebe M, Moriyama Y, Maeda M, Futai M. Molecular cloning of cDNA encoding the 16 KDa subunit of vacuolar H(+)-ATPase from mouse cerebellum. Biochem Biophys Res Commun. 1991;176:1062-7 pubmed
    ..The subunit had four transmembrane segments: Segment II and IV were highly homologous and Glu-139 was located in Segment IV. The roles of the non-conserved regions are discussed. ..
  3. Natsumeda Y, Ohno S, Kawasaki H, Konno Y, Weber G, Suzuki K. Two distinct cDNAs for human IMP dehydrogenase. J Biol Chem. 1990;265:5292-5 pubmed
    ..This is the first report suggesting the existence of two distinct types of human IMP dehydrogenase molecular species which may have different sensitivities to the drugs targeted against IMP dehydrogenase. ..
  4. Ichikawa N, Yoshida Y, Hashimoto T, Ogasawara N, Yoshikawa H, Imamoto F, et al. Activation of ATP hydrolysis by an uncoupler in mutant mitochondria lacking an intrinsic ATPase inhibitor in yeast. J Biol Chem. 1990;265:6274-8 pubmed
    ..These observations suggest that the ATPase inhibitor inhibits ATP hydrolysis by F1F0-ATPase only when the membrane potential is lost. ..
  5. Ogura T, Bouloc P, Niki H, D Ari R, Hiraga S, Jaffe A. Penicillin-binding protein 2 is essential in wild-type Escherichia coli but not in lov or cya mutants. J Bacteriol. 1989;171:3025-30 pubmed
    ..In a cya mutant lacking PBP2, the restoration of a Cya+ phenotype by addition of cyclic AMP caused lethality and a block in cell division. These results show that in wild-type cells, PBP2 is essential for growth and division. ..
  6. Joh T, Takeshima H, Tsuzuki T, Setoyama C, Shimada K, Tanase S, et al. Cloning and sequence analysis of cDNAs encoding mammalian cytosolic malate dehydrogenase. Comparison of the amino acid sequences of mammalian and bacterial malate dehydrogenase. J Biol Chem. 1987;262:15127-31 pubmed
  7. Asai Y, Inui M, Vertes A, Kobayashi M, Yukawa H. Cloning and sequence determination of the aspartase-encoding gene from Brevibacterium flavum MJ233. Gene. 1995;158:87-90 pubmed
    ..coli, Bacillus subtilis and Pseudomonas fluorescens revealed 63, 47 and 57% homology, respectively. The aspA product was determined to have a size of approx. 57 kDa by SDS-PAGE. ..
  8. Masuda Y, Ohtsubo E. Mapping and disruption of the chpB locus in Escherichia coli. J Bacteriol. 1994;176:5861-3 pubmed
    ..7 min on the map of Escherichia coli. We then constructed mutants with an insertion of a (cat) fragment within chpBK or chpBI on the E. coli chromosome. These mutants grew normally, indicating that chpB is dispensable for cell growth. ..
  9. Nureki O, Niimi T, Muramatsu T, Kanno H, Kohno T, Florentz C, et al. Molecular recognition of the identity-determinant set of isoleucine transfer RNA from Escherichia coli. J Mol Biol. 1994;236:710-24 pubmed
    ..Thus, it is proposed that the recognition by IleRS of all the widely distributed identity determinants is coupled with a global conformational change that involves the loosening of a particular set of tertiary base-pairs of tRNA(Ile). ..

More Information

Publications472 found, 100 shown here

  1. Shirai Y, Akiyama Y, Ito K. Suppression of ftsH mutant phenotypes by overproduction of molecular chaperones. J Bacteriol. 1996;178:1141-5 pubmed
    ..These results suggest that FtsH functions can be somehow compensated for when the cellular concentrations of some molecular chaperones increase. ..
  2. Kawata Y, Mimuro J, Kaneko M, Shimada K, Sakata Y. Expression of plasminogen activator inhibitor 2 in the adult and embryonic mouse tissues. Thromb Haemost. 1996;76:569-76 pubmed
    ..These data suggest that PAI-2 may play a role in protecting the mouse embryo from the protease attack in the amniotic fluid and also in maintaining and/or protecting the structure of various surfaces in the mouse. ..
  3. Takeyama H, Takeda D, Yazawa K, Yamada A, Matsunaga T. Expression of the eicosapentaenoic acid synthesis gene cluster from Shewanella sp. in a transgenic marine cyanobacterium, Synechococcus sp. Microbiology. 1997;143 ( Pt 8):2725-31 pubmed
    ..The yield was further improved to 0.64 mg (g dry weight)-1 by incubation for 1 d at 17 degrees C. This is believed to be the first successful cloning and expression of such a large heterologous gene cluster in a marine cyanobacterium. ..
  4. Horie N, Yamaizumi Z, Kuchino Y, Takai K, Goldman E, Miyazawa T, et al. Modified nucleosides in the first positions of the anticodons of tRNA(Leu)4 and tRNA(Leu)5 from Escherichia coli. Biochemistry. 1999;38:207-17 pubmed
    ..Because of the conformational rigidity of Cm and cmnm5Um in the first position of the anticodon, these tRNA(Leu) species recognize the leucine codons UUA++ and UUG correctly, but never recognize the phenylalanine codons UUU and UUC. ..
  5. Tanabe M, Nishio K, Iko Y, Sambongi Y, Iwamoto Kihara A, Wada Y, et al. Rotation of a complex of the gamma subunit and c ring of Escherichia coli ATP synthase. The rotor and stator are interchangeable. J Biol Chem. 2001;276:15269-74 pubmed
    ..These results indicate that the gammaepsilonc(10-14) complex is a mechanical unit of the enzyme and that it can be used as a rotor or a stator experimentally, depending on the subunit immobilized. ..
  6. Hamano Y, Dairi T, Yamamoto M, Kawasaki T, Kaneda K, Kuzuyama T, et al. Cloning of a gene cluster encoding enzymes responsible for the mevalonate pathway from a terpenoid-antibiotic-producing Streptomyces strain. Biosci Biotechnol Biochem. 2001;65:1627-35 pubmed
    ..coli. Among them, the recombinant GGDPS, MK, and IPP isomerase were confirmed to have the expected activities. This is the first report, to the best of our knowledge, about eubacterial MK with direct evidence. ..
  7. Takeuchi A, Matsumura H, Kano Y. Cloning and expression in Escherichia coli of a gene, hup, encoding the histone-like protein HU of Bifidobacterium longum. Biosci Biotechnol Biochem. 2002;66:598-603 pubmed
    ..Incapabilities of Mu phage propagation in an E. coli mutant deficient in HU or IHF could be suppressed by DNA bearing this orf. These results showed that the orf is a gene hup encoding HBI, a histone-like protein HU of B. longum. ..
  8. Yamakawa H, Katou S, Seo S, Mitsuhara I, Kamada H, Ohashi Y. Plant MAPK phosphatase interacts with calmodulins. J Biol Chem. 2004;279:928-36 pubmed publisher
    ..These results suggest that plant CaMs are involved in these stress-activated MAPK cascades via NtMKP1...
  9. Inagaki H, Akagi M, Imai H, Taylor R, Kubo T. Molecular cloning and biological characterization of novel antimicrobial peptides, pilosulin 3 and pilosulin 4, from a species of the Australian ant genus Myrmecia. Arch Biochem Biophys. 2004;428:170-8 pubmed
    ..Synthetic pilosulin 3 and pilosulin 4 peptides displayed antimicrobial activity with histamine-releasing and low hemolytic activities. ..
  10. Maeda H, Miyata T, Kusakisako K, Galay R, Talactac M, Umemiya Shirafuji R, et al. A novel C-type lectin with triple carbohydrate recognition domains has critical roles for the hard tick Haemaphysalis longicornis against Gram-negative bacteria. Dev Comp Immunol. 2016;57:38-47 pubmed publisher
    ..Furthermore, E. coli injection after silencing of HlCLec caused drastic reduction in survival rate of ticks. These results strongly suggest the key role of HlCLec in tick innate immunity against Gram-negative bacteria. ..
  11. Hiratsu K, Nakata A, Shinagawa H, Makino K. Autophosphorylation and activation of transcriptional activator PhoB of Escherichia coli by acetyl phosphate in vitro. Gene. 1995;161:7-10 pubmed
    ..These results indicate that PhoB is an enzyme that catalyzes its own phosphorylation using acP, a low-molecular-weight metabolic intermediate. ..
  12. Ukita T, Ikeda H. Role of the recJ gene product in UV-induced illegitimate recombination at the hotspot. J Bacteriol. 1996;178:2362-7 pubmed
    ..We propose a model based on the 5'-3' exonuclease activity of RecJ to explain the involvement of this protein in illegitimate recombination at the hotspot. ..
  13. Hiraga K, Yutani K. Study of cysteine residues in the alpha subunit of Escherichia coli tryptophan synthase. 1. Role in conformational stability. Protein Eng. 1996;9:425-31 pubmed
  14. Ueguchi C, Ito K. Multicopy suppression: an approach to understanding intracellular functioning of the protein export system. J Bacteriol. 1992;174:1454-61 pubmed
    ..Possible mechanisms of suppression as well as the significance and limitations of the multicopy suppression approach are discussed. ..
  15. Nureki O, Suzuki K, Hara Yokoyama M, Kohno T, Matsuzawa H, Ohta T, et al. Glutamyl-tRNA synthetase from Thermus thermophilus HB8. Molecular cloning of the gltX gene and crystallization of the overproduced protein. Eur J Biochem. 1992;204:465-72 pubmed
    ..The crystals diffract X-rays beyond 0.35 nm. The crystal belongs to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters of a = 8.64 nm, b = 8.86 nm and c = 8.49 nm. ..
  16. Ishijima S, Kita K, Ahmad I, Ishizuka T, Taira M, Tatibana M. Expression of rat phosphoribosylpyrophosphate synthetase subunits I and II in Escherichia coli. Isolation and characterization of the recombinant isoforms. J Biol Chem. 1991;266:15693-7 pubmed
    ..Inhibition of rPRS I and rPRS II by 0.3 mM ADP was 87 and 54%, respectively, and inhibition by 1 mM GDP was 93 and 24%, respectively. rPRS II was 180-fold more sensitive than rPRS I to heat inactivation at 49 degrees C. ..
  17. Terada K, Izui K. Site-directed mutagenesis of the conserved histidine residue of phosphoenolpyruvate carboxylase. His138 is essential for the second partial reaction. Eur J Biochem. 1991;202:797-803 pubmed
    ..Mutation of His579 to asparagine (H579N) produced an enzyme which had 69% of the wild-type carboxylase activity, but its affinity for P-pyruvate was decreased by 24-fold. ..
  18. Hirono I, Aoki T. Nucleotide sequence and expression of an extracellular hemolysin gene of Aeromonas hydrophila. Microb Pathog. 1991;11:189-97 pubmed
    ..salmonicida (isolated from fish). Three hemolysin-negative strains of A. hydrophila did not react with the AHH1 probe, whereas three non-hemolytic A. salmonicida strains hybridized with the probe. ..
  19. Makino K, Kim S, Shinagawa H, Amemura M, Nakata A. Molecular analysis of the cryptic and functional phn operons for phosphonate use in Escherichia coli K-12. J Bacteriol. 1991;173:2665-72 pubmed
    ..Studies of the structure, regulation, and function of the phn region suggest that the phosphate starvation-inducible phn operon consists of 14 cistrons from phnC to phnP. ..
  20. Nara T, Lee L, Imae Y. Thermosensing ability of Trg and Tap chemoreceptors in Escherichia coli. J Bacteriol. 1991;173:1120-4 pubmed
    ..These results indicate that although both Trg and Tap have the ability to sense thermal stimuli, Trg functions as a warm receptor, as reported previously for Tar and Tsr, while Tap functions as a cold receptor. ..
  21. Minagawa J, Nakamura H, Yamato I, Mogi T, Anraku Y. Transcriptional regulation of the cytochrome b562-o complex in Escherichia coli. Gene expression and molecular characterization of the promoter. J Biol Chem. 1990;265:11198-203 pubmed
  22. Yamada M, Makino K, Shinagawa H, Nakata A. Regulation of the phosphate regulon of Escherichia coli: properties of phoR deletion mutants and subcellular localization of PhoR protein. Mol Gen Genet. 1990;220:366-72 pubmed
    ..The results suggest that PhoR protein is anchored to the cytoplasmic membrane by the amino-terminal region. ..
  23. Iwasaki H, Shiba T, Makino K, Nakata A, Shinagawa H. Overproduction, purification, and ATPase activity of the Escherichia coli RuvB protein involved in DNA repair. J Bacteriol. 1989;171:5276-80 pubmed
    ..RuvB protein had a stronger affinity to ADP than to ATP and weak ATPase activity. The results suggest that the weak ATPase activity of RuvB protein is at least partly due to end product inhibition by ADP. ..
  24. Choi Y, Kawamukai M, Utsumi R, Sakai H, Komano T. Molecular cloning and sequencing of the glycogen phosphorylase gene from Escherichia coli. FEBS Lett. 1989;243:193-8 pubmed
    ..The enzyme activity of glycogen phosphorylase increased after introduction on a multicopy of the glgP gene. ..
  25. Nohno T, Noji S, Taniguchi S, Saito T. The narX and narL genes encoding the nitrate-sensing regulators of Escherichia coli are homologous to a family of prokaryotic two-component regulatory genes. Nucleic Acids Res. 1989;17:2947-57 pubmed
    ..Presence of two copies of hydrophobic segments in the N-terminal half of the NarX protein suggests the role as a transmembrane receptor sensing nitrate. ..
  26. Yamagata S, Isaji M, Nakamura K, Fujisaki S, Doi K, Bawden S, et al. Overexpression of the Saccharomyces cerevisiae MET17/MET25 gene in Escherichia coli and comparative characterization of the product with O-acetylserine.O-acetylhomoserine sulfhydrylase of the yeast. Appl Microbiol Biotechnol. 1994;42:92-9 pubmed
    ..In contrast to a previous report we found that cystathionine gamma-lyase of S. ..
  27. Yamanaka H, Kameyama M, Baba T, Fujii Y, Okamoto K. Maturation pathway of Escherichia coli heat-stable enterotoxin I: requirement of DsbA for disulfide bond formation. J Bacteriol. 1994;176:2906-13 pubmed
    ..We conclude that Cys-39 is important for recognition by the processing enzymes required for the maturation of STp. ..
  28. Sekizaki T, Ito H, Asawa T, Nonomura I. DNA sequence of type 1 fimbrin, Fpul1, gene from a chicken pathogenic Escherichia coli serotype O78. J Vet Med Sci. 1993;55:395-400 pubmed
    ..The E. coli K-12 strains carrying the Fpul1 genes did not show the acid-induced autoagglutination, suggesting that the Fpul1 was genetically distinct from the acid-induced autoagglutination. ..
  29. Nagadoi A, Morikawa S, Nakamura H, Enari M, Kobayashi K, Yamamoto H, et al. Structural comparison of the free and DNA-bound forms of the purine repressor DNA-binding domain. Structure. 1995;3:1217-24 pubmed
    ..The dimerization of the hinge helices is likely to be controlled by the CBD dimerization interface, but is induced by specific-DNA binding...
  30. Ito K, Nakamura Y. Localization of nusA-suppressing amino acid substitutions in the conserved regions of the beta' subunit of Escherichia coli RNA polymerase. Mol Gen Genet. 1996;251:699-706 pubmed
    ..These results suggested that the conserved domains of the beta' subunit of E. coli RNA polymerase are involved in transcript termination or interaction with termination factor(s). ..
  31. Azakami H, Yumoto H, Nakae H, Matsuo T, Ebisu S. Molecular analysis of the gene encoding a protein component of the Eikenella corrodens adhesin complex that is close to the carbohydrate recognition domain. Gene. 1996;180:207-12 pubmed
  32. Matsuda K, Mizuguchi K, Nishioka T, Kato H, Go N, Oda J. Crystal structure of glutathione synthetase at optimal pH: domain architecture and structural similarity with other proteins. Protein Eng. 1996;9:1083-92 pubmed
    ..A structural motif in the N-terminal domain of GSHase has been found to be similar to the NAD-binding fold. This structural motif is shared by a number of other proteins that bind various negatively charged molecules. ..
  33. Tanabe O, Gomez G, Nishito Y, Usui H, Takeda M. Molecular heterogeneity of the cDNA encoding a 74-kDa regulatory subunit (B" or delta) of human protein phosphatase 2A. FEBS Lett. 1997;408:52-6 pubmed
    ..Using isoform-specific antipeptide antisera, the 74-kDa subunit (B" or delta) originally purified from human erythrocytes was identified as the delta1 isoform. ..
  34. Okumura N, Masamoto K, Wada H. The gshB gene in the cyanobacterium Synechococcus sp. PCC 7942 encodes a functional glutathione synthetase. Microbiology. 1997;143 ( Pt 9):2883-90 pubmed
    ..These findings demonstrate that the gshB gene of Synechococcus sp. PCC 7942 is a structural gene for glutathione synthetase and is involved in the biosynthesis of glutathione. ..
  35. Kobashi N, Nishiyama M, Yamane H. Characterization of aspartate kinase III of Bacillus subtilis. Biosci Biotechnol Biochem. 2001;65:1391-4 pubmed
    ..5) buffer, but was stabilized by addition of 500 mM ammonium sulfate. Although all the aspartate kinases so far investigated are oligomeric enzymes, this aspartate kinase was suggested to be a monomer. ..
  36. Miyamoto Y, Machida K, Mizunuma M, Emoto Y, Sato N, Miyahara K, et al. Identification of Saccharomyces cerevisiae isoleucyl-tRNA synthetase as a target of the G1-specific inhibitor Reveromycin A. J Biol Chem. 2002;277:28810-4 pubmed
    ..IleRS inhibition by RM-A led to transcriptional activation of the ILS1 gene via the Gcn2-Gcn4 general amino acid control pathway, and this autoregulation seemed to contribute to RM-A resistance. ..
  37. Shiomi D, Homma M, Kawagishi I. Intragenic suppressors of a mutation in the aspartate chemoreceptor gene that abolishes binding of the receptor to methyltransferase. Microbiology. 2002;148:3265-75 pubmed
    ..This effect was reversed by introducing a mutation in the xWxxF motif. These results reinforce the importance of the xWxxF motif and suggest that the motif does not have to be located at the extreme carboxy terminus. ..
  38. Gomi K, Yamasaki Y, Yamamoto H, Akimitsu K. Characterization of a hydroperoxide lyase gene and effect of C6-volatiles on expression of genes of the oxylipin metabolism in Citrus. J Plant Physiol. 2003;160:1219-31 pubmed
  39. Momma M, Fujimoto Z. Interdomain disulfide bridge in the rice granule bound starch synthase I catalytic domain as elucidated by X-ray structure analysis. Biosci Biotechnol Biochem. 2012;76:1591-5 pubmed
    ..The presence of three covalent linkages might explain why both OsGBSSI-CD structures adopted only the closed domain arrangement. ..
  40. Ota E, Tsuchiya W, Yamazaki T, Nakamura M, Hirayama C, Konno K. Purification, cDNA cloning and recombinant protein expression of a phloem lectin-like anti-insect defense protein BPLP from the phloem exudate of the wax gourd, Benincasa hispida. Phytochemistry. 2013;89:15-25 pubmed publisher
  41. Gozu Y, Ishizaki Y, Hosoyama Y, Miyazaki T, Nishikawa A, Tonozuka T. A glycoside hydrolase family 31 dextranase with high transglucosylation activity from Flavobacterium johnsoniae. Biosci Biotechnol Biochem. 2016;80:1562-7 pubmed publisher
    ..The optimal pH and temperature were pH 6.0 and 60 °C, respectively. Our results indicate that Fjoh_4430 is a novel GH31 dextranase with high transglucosylation activity. ..
  42. Hashimoto W, Suzuki H, Nohara S, Kumagai H. Escherichia coli gamma-glutamyltranspeptidase mutants deficient in processing to subunits. Biochem Biophys Res Commun. 1992;189:173-8 pubmed
    ..On Western blot analysis, we found that both mutants accumulated a gamma-glutamyltranspeptidase precursor which was not processed into large and small subunits in the periplasmic space of Escherichia coli. ..
  43. Yasuzawa K, Hayashi N, Goshima N, Kohno K, Imamoto F, Kano Y. Histone-like proteins are required for cell growth and constraint of supercoils in DNA. Gene. 1992;122:9-15 pubmed
    ..The bgl operon was expressed in cells depleted of both HU and H-NS as well as in cells depleted of H-NS.(ABSTRACT TRUNCATED AT 250 WORDS) ..
  44. Mizushima K, Awakihara S, Kuroda M, Ishikawa T, Tsuda M, Tsuchiya T. Cloning and sequencing of the melB gene encoding the melibiose permease of Salmonella typhimurium LT2. Mol Gen Genet. 1992;234:74-80 pubmed
  45. Yamada M, Shahjahan M, Tanabe T, Kishi F, Nakazawa A. Cloning and characterization of cDNA for mitochondrial GTP:AMP phosphotransferase of bovine liver. J Biol Chem. 1989;264:19192-9 pubmed
    ..coli. The AK3 product was exported to the periplasmic space through the bacterial inner membrane. The possible involvement of the NH2-terminal sequence of the protein in targeting to the mitochondrial matrix was discussed. ..
  46. Tanaka S, Matsushita Y, Yoshikawa A, Isono K. Cloning and molecular characterization of the gene rimL which encodes an enzyme acetylating ribosomal protein L12 of Escherichia coli K12. Mol Gen Genet. 1989;217:289-93 pubmed
    ..The significance of these results with respect to other acetylating enzymes, in particular those involved in the acetylation of aminoglycoside antibiotics is discussed. ..
  47. Hosoya H, Nakamura K. DNA sequence of proline permease gene from Pseudomonas fluorescens and predicted structure of proline permease. Biosci Biotechnol Biochem. 1994;58:2099-101 pubmed
    ..The DNA sequence is 71.0% and 70.7% identical and the amino acid sequence is 75.8% and 76.0% similar to those of Escherichia coli and Salmonella typhimurium, respectively. ..
  48. Tsumoto K, Nakaoki Y, Ueda Y, Ogasahara K, Yutani K, Watanabe K, et al. Effect of the order of antibody variable regions on the expression of the single-chain HyHEL10 Fv fragment in E. coli and the thermodynamic analysis of its antigen-binding properties. Biochem Biophys Res Commun. 1994;201:546-51 pubmed
    ..Thermodynamic study showed that this reduced activity was due to entropic loss upon binding to its antigen, although this interaction between scFvLH and its antigen was enthalpically favorable. ..
  49. Baba T, Taura T, Shimoike T, Akiyama Y, Yoshihisa T, Ito K. A cytoplasmic domain is important for the formation of a SecY-SecE translocator complex. Proc Natl Acad Sci U S A. 1994;91:4539-43 pubmed
    ..These results indicate that the secY24 mutation weakens SecY's interaction with SecE. Taken together, we propose that cytoplasmic domain 4 is important for the association between SecY and SecE. ..
  50. Tanaka T, Oda J, Yamaguchi H, Katsube Y. [Structural and functional analysis on glutathione-synthetase]. Tanpakushitsu Kakusan Koso. 1993;38:1579-88 pubmed
  51. Uno M, Ito K, Nakamura Y. Functional specificity of amino acid at position 246 in the tRNA mimicry domain of bacterial release factor 2. Biochimie. 1996;78:935-43 pubmed
  52. Tanaka N, Hiyama T, Nakamoto H. Cloning, characterization and functional analysis of groESL operon from thermophilic cyanobacterium Synechococcus vulcanus. Biochim Biophys Acta. 1997;1343:335-48 pubmed
    ..Introduction of the cloned groEL1 gene into a groEL defective mutant of E. coli resulted in the complementation of heat sensitivity, which contrasted with the previous result with groEL2...
  53. Morita T, Hayashi M. 1,4-Dioxane is not mutagenic in five in vitro assays and mouse peripheral blood micronucleus assay, but is in mouse liver micronucleus assay. Environ Mol Mutagen. 1998;32:269-80 pubmed
    ..It is also conceivable that the positive result in mouse liver micronucleus assay was due to a nongenotoxic mechanism, i.e., errors in genetic repair following enhancement of hepatocyte proliferation. ..
  54. Tanaka T, Saha S, Tomomori C, Ishima R, Liu D, Tong K, et al. NMR structure of the histidine kinase domain of the E. coli osmosensor EnvZ. Nature. 1998;396:88-92 pubmed publisher
    ..This core has a novel protein kinase structure, distinct from the serine/threonine/tyrosine kinase fold, with unanticipated similarities to both heatshock protein 90 and DNA gyrase B...
  55. Mizutani Y, Tamiya Koizumi K, Irie F, Hirabayashi Y, Miwa M, Yoshida S. Cloning and expression of rat neutral sphingomyelinase: enzymological characterization and identification of essential histidine residues. Biochim Biophys Acta. 2000;1485:236-46 pubmed
    ..This enzyme also catalyzed the hydrolysis of lyso-platelet activating factor to yield 1-alkylglycerol at a rate that is slightly lower than that with sphingomyelin. ..
  56. Matsuoka H, Oishi K, Watanabe M, Kozone I, Saito M, Igimi S. Viable cell detection by the combined use of fluorescent glucose and fluorescent glycine. Biosci Biotechnol Biochem. 2003;67:2459-62 pubmed
    ..Thirty-five out of 41 strains showed marked uptake of 2NBDG but 6 strains were not able to take in 2NBDG. Five out of these 6 strains showed NBD-Gly uptake. ..
  57. Morishige T, Choi K, Sato F. In vivo bioconversion of tetrahydroisoquinoline by recombinant coclaurine N-methyltransferase. Biosci Biotechnol Biochem. 2004;68:939-41 pubmed
    ..Transgenic E. coli successfully N-methylated the substrate added to the medium and excreted the product. Limitation of bioconversion by the supply of methyl-group donor is discussed. ..
  58. Inomata K, Hammam M, Kinoshita H, Murata Y, Khawn H, Noack S, et al. Sterically locked synthetic bilin derivatives and phytochrome Agp1 from Agrobacterium tumefaciens form photoinsensitive Pr- and Pfr-like adducts. J Biol Chem. 2005;280:24491-7 pubmed
    ..In this way, physiological action of Pfr can be studied in vivo and separated from Pr/Pfr cycling and other light effects. ..
  59. Hiroi M, Yamazaki F, Harada T, Takahashi N, Iida N, Noda Y, et al. Prevalence of extended-spectrum ?-lactamase-producing Escherichia coli and Klebsiella pneumoniae in food-producing animals. J Vet Med Sci. 2012;74:189-95 pubmed
    ..CTX-M-producing coliforms in broilers should be controlled due to the critical importance of cephalosporins and the zoonotic potential of ESBL-producing bacteria. ..
  60. Nakai T, Ono K, Kuroda S, Tanizawa K, Okajima T. An unusual subtilisin-like serine protease is essential for biogenesis of quinohemoprotein amine dehydrogenase. J Biol Chem. 2012;287:6530-8 pubmed publisher
    ..These results demonstrate that ORF5 is essential for QHNDH biogenesis, serving as a processing protease to cleave the γ subunit leader peptide nearly in a disposable manner. ..
  61. Hagiwara M, Komatsu T, Sugiura S, Isoda R, Tada H, Tanigawa N, et al. POT1b regulates phagocytosis and NO production by modulating activity of the small GTPase Rab5. Biochem Biophys Res Commun. 2013;439:413-7 pubmed publisher
    ..These results suggest that POT1b negatively regulates phagocytosis by controlling Rab5 activity and thereby modulates bacteria-induced NO generation. These findings suggest that POT1b participates in innate immune responses. ..
  62. Honda K, Hara N, Cheng M, Nakamura A, Mandai K, Okano K, et al. In vitro metabolic engineering for the salvage synthesis of NAD(.). Metab Eng. 2016;35:114-120 pubmed publisher
    ..When incubated with experimentally optimized concentrations of the enzymes at 60°C, the NAD(+) concentration could be kept almost constant for 15h. ..
  63. Date T, Yamaguchi M, Hirose F, Nishimoto Y, Tanihara K, Matsukage A. Expression of active rat DNA polymerase beta in Escherichia coli. Biochemistry. 1988;27:2983-90 pubmed
  64. Tamai E, Belyaeva T, Busby S, Tsuchiya T. Mutations that increase the activity of the promoter of the Escherichia coli melibiose operon improve the binding of MelR, a transcription activator triggered by melibiose. J Biol Chem. 2000;275:17058-63 pubmed
    ..Thus, the activity of the melAB promoter is fixed by the occupation by MelR of a DNA site that overlaps the -35 hexamer: MelR appears to be a typical class II-type transcription activator. ..
  65. Nakamura N, Shigematsu A, Matsunaga T. Electrochemical detection of viable bacteria in urine and antibiotic selection. Biosens Bioelectron. 1991;6:575-80 pubmed
    ..The minimum inhibitory concentration values obtained by the electrochemical method were in good agreement with those obtained by the conventional method. ..
  66. Nakashima K, Kanamaru K, Aiba H, Mizuno T. Signal transduction and osmoregulation in Escherichia coli. A novel type of mutation in the phosphorylation domain of the activator protein, OmpR, results in a defect in its phosphorylation-dependent DNA binding. J Biol Chem. 1991;266:10775-80 pubmed
    ..The mutant proteins characterized in this study seem to be defective in this particular oligomerization process observed in vitro. ..
  67. Ikeda M, Wachi M, Jung H, Ishino F, Matsuhashi M. Nucleotide sequence involving murG and murC in the mra gene cluster region of Escherichia coli. Nucleic Acids Res. 1990;18:4014 pubmed
  68. Asai T, Takanami M, Imai M. The AT richness and gid transcription determine the left border of the replication origin of the E. coli chromosome. EMBO J. 1990;9:4065-72 pubmed
    ..It is presumed that gid transcription introduces negative superhelicity at the AT rich region and facilitates DnaA dependent duplex opening. ..
  69. Hirano T, Konoha G, Toda T, Yanagida M. Essential roles of the RNA polymerase I largest subunit and DNA topoisomerases in the formation of fission yeast nucleolus. J Cell Biol. 1989;108:243-53 pubmed
  70. Yamada M, Sasakawa C, Okada N, Makino S, Yoshikawa M. Molecular cloning and characterization of chromosomal virulence region kcpA of Shigella flexneri. Mol Microbiol. 1989;3:207-13 pubmed
  71. Makino K, Shinagawa H, Amemura M, Kimura S, Nakata A, Ishihama A. Regulation of the phosphate regulon of Escherichia coli. Activation of pstS transcription by PhoB protein in vitro. J Mol Biol. 1988;203:85-95 pubmed
    ..Our findings indicate that PhoB protein recognizes and binds to the pho box and activates transcription of the genes in the phosphate regulon. ..
  72. Yoshimura T, Ashiuchi M, Esaki N, Kobatake C, Choi S, Soda K. Expression of glr (murI, dga) gene encoding glutamate racemase in Escherichia coli. J Biol Chem. 1993;268:24242-6 pubmed
    ..We partially purified the enzyme to characterize it. The enzyme consists of two identical subunits with a molecular weight of about 31,000 in contrast to the P. pentosaceus enzyme, a monomer protein. ..
  73. Akiyama Y, Ogura T, Ito K. Involvement of FtsH in protein assembly into and through the membrane. I. Mutations that reduce retention efficiency of a cytoplasmic reporter. J Biol Chem. 1994;269:5218-24 pubmed
    ..These results suggest that FtsH participates in assembly of proteins into and through the membrane. It is needed for the cell to assure efficient stop-transfer of some transmembrane proteins. ..
  74. Nakamura S, Sakurai T, Nonomura Y. Differential expression of bovine adseverin in adrenal gland revealed by in situ hybridization. Cloning of a cDNA for adseverin. J Biol Chem. 1994;269:5890-6 pubmed
    ..The observation strongly suggested that adseverin is not involved in secretory processes in general but is specifically involved in exocytosis. ..
  75. Hiraga K, Yutani K. A thermodynamic analysis of conformational change due to the alpha 2 beta 2 complex formation of tryptophan synthase. Eur J Biochem. 1996;240:63-70 pubmed
    ..The analysis of the thermodynamic parameters of association suggested that the complex formation couples with the folding (rearrangements) of the alpha subunit monomer or/and beta subunit dimer. ..
  76. Ohya Y, Caplin B, Qadota H, Tibbetts M, Anraku Y, Pringle J, et al. Mutational analysis of the beta-subunit of yeast geranylgeranyl transferase I. Mol Gen Genet. 1996;252:1-10 pubmed
    ..Thus, several phenotypic differences are observed among the cal1/cdc43 mutations, possibly due to alterations in substrate specificity caused by the mutations. ..
  77. Machida S, Yu Y, Singh S, Kim J, Hayashi K, Kawata Y. Overproduction of beta-glucosidase in active form by an Escherichia coli system coexpressing the chaperonin GroEL/ES. FEMS Microbiol Lett. 1998;159:41-6 pubmed
    ..Similar effects of GroEL/ES were also observed on the overexpressed beta-glucosidase from Agrobacterium tumefaciens...
  78. Matsumura T, Kimata Ariga Y, Sakakibara H, Sugiyama T, Murata H, Takao T, et al. Complementary DNA cloning and characterization of ferredoxin localized in bundle-sheath cells of maize leaves. Plant Physiol. 1999;119:481-8 pubmed
    ..These structural and functional differences of Fd I and Fd II may be related to their cell-specific expression in the leaves of a C4 plant. ..
  79. Ohashi E, Ogi T, Kusumoto R, Iwai S, Masutani C, Hanaoka F, et al. Error-prone bypass of certain DNA lesions by the human DNA polymerase kappa. Genes Dev. 2000;14:1589-94 pubmed
    ..Therefore, our results implicate an important role for polkappa in the mutagenic bypass of certain types of DNA lesions. ..
  80. Aono S, Kato T, Matsuki M, Nakajima H, Ohta T, Uchida T, et al. Resonance Raman and ligand binding studies of the oxygen-sensing signal transducer protein HemAT from Bacillus subtilis. J Biol Chem. 2002;277:13528-38 pubmed
    ..Based on these results, the structural and functional properties of HemAT-Bs are discussed. ..
  81. Uchimura H, Enjoji H, Seki T, Taguchi A, Takaya N, Shoun H. Nitrate reductase-formate dehydrogenase couple involved in the fungal denitrification by Fusarium oxysporum. J Biochem. 2002;131:579-86 pubmed
    ..Analyses of the mutant species of F. oxysporum that were defective in Nar and/or assimilatory nitrate reductase conclusively showed that Nar is essential for the fungal denitrification. ..
  82. Hanada N, Fukushima K, Nomura Y, Senpuku H, Hayakawa M, Mukasa H, et al. Cloning and nucleotide sequence analysis of the Streptococcus sobrinus gtfU gene that produces a highly branched water-soluble glucan. Biochim Biophys Acta. 2002;1570:75-9 pubmed
    ..coli MD66 clone was similar to S. sobrinus GTF-S1. Biological properties and a linkage analysis of the glucans by 13C NMR spectrometry revealed that the protein produced by the E. coli MD66 clone was GTF-S1...
  83. Chung J, Fujii I, Harada S, Sankawa U, Ebizuka Y. Expression, purification, and characterization of AknX anthrone oxygenase, which is involved in aklavinone biosynthesis in Streptomyces galilaeus. J Bacteriol. 2002;184:6115-22 pubmed
    ..A possible mechanism for the reaction via peroxy anion intermediate is proposed. ..
  84. Ueno H, Yonesaki T. Role of Escherichia coli Hfq in late-gene silencing of bacteriophage T4 dmd mutant. Genes Genet Syst. 2002;77:301-8 pubmed
    ..We also showed that purified Hfq protein bound stoichiometrically to soc RNA. These results strongly suggest that the hfq gene has a stimulatory role in dmd mutant-specific mRNA degradation...
  85. Sugiyama M, Suzuki S, Tonouchi N, Yokozeki K. Cloning of the xylitol dehydrogenase gene from Gluconobacter oxydans and improved production of xylitol from D-arabitol. Biosci Biotechnol Biochem. 2003;67:584-91 pubmed
    ..These results demonstrated that increasing XDH activity in G. oxydans improved xylitol productivity...
  86. Sugiyama T, Kittaka A, Takayama H, Tomioka M, Ida Y, Kuroda R. Aggregation of RecA-derived peptides on single-stranded oligonucleotides triggered by schiff base-mediated crosslinking. Bioorg Med Chem Lett. 2003;13:2847-51 pubmed
    ..Circular dichroism (CD) spectroscopy, gel mobility shift assay and sedimentation experiment demonstrated that crosslinking reaction proceeded efficiently only when the peptides bound to the oligonucleotides. ..
  87. Mizuno K, Iida T, Takano A, Yokoyama M, Fujimura T. A new 9-lipoxygenase cDNA from developing rice seeds. Plant Cell Physiol. 2003;44:1168-75 pubmed
    ..The restriction enzyme map of the reported genomic sequence agreed with the result of the Southern blot analysis for the r9-LOX1. The enzyme could be useful for in vitro synthesis of 9,10-ketol-octadecadienoic acid...
  88. Kamata Y, Miyanomae A, Nakayama E, Miyanomae T, Tajima T, Hoshi H. Characterization of dog allergens Can f 1 and Can f 2. 1. Preparation of their recombinant proteins and antibodies. Int Arch Allergy Immunol. 2007;142:291-300 pubmed
    ..The significance of dog skin as the tissue producing dog allergens, especially Can f 2, should be considered in further studies. ..
  89. Kadokura H, Beckwith J. Mechanisms of oxidative protein folding in the bacterial cell envelope. Antioxid Redox Signal. 2010;13:1231-46 pubmed publisher
    ..We review here the marked progress in this field and discuss important questions that remain for future studies. ..
  90. Sugino H, Usui T, Shimada T, Nakano M, Ogasawara H, Ishihama A, et al. A structural sketch of RcdA, a transcription factor controlling the master regulator of biofilm formation. FEBS Lett. 2017;591:2019-2031 pubmed publisher
    ..These results provide insights into the DNA-binding mode of RcdA. ..
  91. Matsunaga T, Nakamura C, Burgess J, Sode K. Gene transfer in magnetic bacteria: transposon mutagenesis and cloning of genomic DNA fragments required for magnetosome synthesis. J Bacteriol. 1992;174:2748-53 pubmed
    ..One of these clones was found to hybridize strongly to regions of the A. magnetotacticum MS-1 chromosome. This is the first report of gene transfer in a magnetic bacterium...
  92. Saiki K, Mogi T, Anraku Y. Heme O biosynthesis in Escherichia coli: the cyoE gene in the cytochrome bo operon encodes a protoheme IX farnesyltransferase. Biochem Biophys Res Commun. 1992;189:1491-7 pubmed
    ..Since the CyoE protein contains the putative allylic polyprenyldiphosphate binding domain, we concluded that the cyoE gene encodes a novel enzyme, protoheme IX farnesyltransferase, essential for heme O biosynthesis. ..