Experts and Doctors on escherichia coli in Germany

Summary

Locale: Germany
Topic: escherichia coli

Top Publications

  1. Nagel K, Voigt J. Regulation of the uncoupled GTPase activity of elongation factor G (EF-G) by the conformations of the ribosomal subunits. Biochim Biophys Acta. 1993;1174:153-61 pubmed
    ..A spontaneous transformation of 'state II' to 'state I' is expected to occur in the absence of mRNA, aminoacyl-tRNA and EF-T especially under salt conditions favouring state I. ..
  2. Huenges M, Rolz C, Gschwind R, Peteranderl R, Berglechner F, Richter G, et al. Solution structure of the antitermination protein NusB of Escherichia coli: a novel all-helical fold for an RNA-binding protein. EMBO J. 1998;17:4092-100 pubmed
    ..The overall packing of the protein appears to be conserved, but the flexible N-terminus adopts a more rigid structure upon RNA binding, indicating that the N-terminus functions as an arginine-rich RNA-binding motif (ARM). ..
  3. Metzner M, Stoller G, Rücknagel K, Lu K, Fischer G, Luckner M, et al. Functional replacement of the essential ESS1 in yeast by the plant parvulin DlPar13. J Biol Chem. 2001;276:13524-9 pubmed
    ..In contrast, the human parvulin hPar14 is not able to rescue the lethal phenotype of this yeast strain at nonpermissive temperatures. These results suggest a function for DlPar13 rather similar to parvulins of the Pin1-type. ..
  4. Nitschke M, Wiehl S, Baer P, Kreft B. Bactericidal activity of renal tubular cells: the putative role of human beta-defensins. Exp Nephrol. 2002;10:332-7 pubmed
    ..This is the first evidence that human RTC are able to produce antibacterial substances in a biologically relevant amount and that beta-defensins are candidate proteins responsible for this effect. ..
  5. Blaudeck N, Kreutzenbeck P, Freudl R, Sprenger G. Genetic analysis of pathway specificity during posttranslational protein translocation across the Escherichia coli plasma membrane. J Bacteriol. 2003;185:2811-9 pubmed
  6. Burga L, Wellmann F, Lukacin R, Witte S, Schwab W, Schröder J, et al. Unusual pseudosubstrate specificity of a novel 3,5-dimethoxyphenol O-methyltransferase cloned from Ruta graveolens L. Arch Biochem Biophys. 2005;440:54-64 pubmed
    ..0 microM. Thus, the OMT exhibited TMT activity with almost equivalent affinity to the thiol pseudosubstrate which is structurally unrelated to methoxyphenols...
  7. Schiffmann R, Neugebauer A, Klein C. Metal-mediated inhibition of Escherichia coli methionine aminopeptidase: structure-activity relationships and development of a novel scoring function for metal-ligand interactions. J Med Chem. 2006;49:511-22 pubmed
    ..Finally, conclusions for the structure-based design of in vivo-active inhibitors of ecMetAP are drawn. ..
  8. Standar K, Mehner D, Osadnik H, Berthelmann F, Hause G, Lünsdorf H, et al. PspA can form large scaffolds in Escherichia coli. FEBS Lett. 2008;582:3585-9 pubmed publisher
    ..The PspA-scaffold may be of fundamental importance, as it could allow PspA to stabilize the integrity of membranes through numerous contact points over a large surface area. ..
  9. Scheu P, Kim O, Griesinger C, Unden G. Sensing by the membrane-bound sensor kinase DcuS: exogenous versus endogenous sensing of C(4)-dicarboxylates in bacteria. Future Microbiol. 2010;5:1383-402 pubmed publisher
    ..Therefore, DcuS senses C(4)-dicarboxylates in two independent modes, responding to the effector concentration and the metabolic flux of extracellular C(4)-dicarboxylates. ..

More Information

Publications652 found, 100 shown here

  1. Sawers G. The hydrogenases and formate dehydrogenases of Escherichia coli. Antonie Van Leeuwenhoek. 1994;66:57-88 pubmed
    ..This article aims to review the current state of knowledge regarding the biochemistry, genetics, molecular biology and physiology of these enzymes. ..
  2. Wehrmann A, Eggeling L, Sahm H. Analysis of different DNA fragments of Corynebacterium glutamicum complementing dapE of Escherichia coli. Microbiology. 1994;140 ( Pt 12):3349-56 pubmed
    ..coli. The C. glutamicum gene now available is the first gene from the succinylase branch of lysine synthesis of this biotechnologically important organism. ..
  3. Hesterkamp T, Hauser S, Lütcke H, Bukau B. Escherichia coli trigger factor is a prolyl isomerase that associates with nascent polypeptide chains. Proc Natl Acad Sci U S A. 1996;93:4437-41 pubmed
    ..We propose that trigger factor is a folding catalyst acting cotranslationally. ..
  4. Jellen Ritter A, Kern W. Enhanced expression of the multidrug efflux pumps AcrAB and AcrEF associated with insertion element transposition in Escherichia coli mutants Selected with a fluoroquinolone. Antimicrob Agents Chemother. 2001;45:1467-72 pubmed
    ..These observations confirm the importance of efflux-associated nontarget gene mutations and indicate that transposition of genetic elements may have a role in the development of fluoroquinolone resistance in E. coli. ..
  5. Müller Schmehl K, Beninde J, Finckh B, Florian S, Dudenhausen J, Brigelius Flohe R, et al. Localization of alpha-tocopherol transfer protein in trophoblast, fetal capillaries' endothelium and amnion epithelium of human term placenta. Free Radic Res. 2004;38:413-20 pubmed
    ..In amnion epithelial cells, however, TTPA was predominantly located in the nuclei. Nuclear localization of the protein may represent a novel function of TTPA. ..
  6. Erbse A, Schmidt R, Bornemann T, Schneider Mergener J, Mogk A, Zahn R, et al. ClpS is an essential component of the N-end rule pathway in Escherichia coli. Nature. 2006;439:753-6 pubmed
    ..Through interaction with this signal, ClpS converts the ClpAP machine into a protease with exquisitely defined specificity, ideally suited to regulatory proteolysis. ..
  7. Kuhnke G, Neumann K, Muhlenhoff U, Lill R. Stimulation of the ATPase activity of the yeast mitochondrial ABC transporter Atm1p by thiol compounds. Mol Membr Biol. 2006;23:173-84 pubmed
    ..We speculate that the physiological substrate of Atm1p may contain multiple sulfhydryl groups in a peptidic environment...
  8. Alber B, Olinger M, Rieder A, Kockelkorn D, Jobst B, H gler M, et al. Malonyl-coenzyme A reductase in the modified 3-hydroxypropionate cycle for autotrophic carbon fixation in archaeal Metallosphaera and Sulfolobus spp. J Bacteriol. 2006;188:8551-9 pubmed publisher
    ..This shows that the autotrophic pathway in Chloroflexus and Sulfolobaceae has evolved convergently and that these taxonomic groups have recruited different genes to bring about similar metabolic processes...
  9. Böhmer C, Böhmer M, Bölker M, Sandrock B. Cdc42 and the Ste20-like kinase Don3 act independently in triggering cytokinesis in Ustilago maydis. J Cell Sci. 2008;121:143-8 pubmed
    ..We propose, that Don3 is involved in establishing a landmark, at which the Cdc42-dependent actomyosin ring formation will occur. ..
  10. Steinbach A, Scheidig A, Klein C. The unusual binding mode of cnicin to the antibacterial target enzyme MurA revealed by X-ray crystallography. J Med Chem. 2008;51:5143-7 pubmed publisher
    ..This adduct, which can be regarded as a noncovalent suicide inhibitor, has been formed by an unusual "anti-Michael" 1,3-addition of UNAG to an alpha,beta-unsaturated carbonyl function in cnicin. ..
  11. Weininger U, Jakob R, Kovermann M, Balbach J, Schmid F. The prolyl isomerase domain of PpiD from Escherichia coli shows a parvulin fold but is devoid of catalytic activity. Protein Sci. 2010;19:6-18 pubmed publisher
    ..In its structure, the parvulin domain of PpiD resembles most closely the inactive first parvulin domain of SurA, which is part of the chaperone unit of this protein and presumably involved in substrate recognition. ..
  12. Schmidt P, Berger C, Scheidt H, Berndt S, Bunge A, Beck Sickinger A, et al. A reconstitution protocol for the in vitro folded human G protein-coupled Y2 receptor into lipid environment. Biophys Chem. 2010;150:29-36 pubmed publisher
    ..The efficiency of the reconstitution could also be inferred from the fact that reasonable (13)C NMR spectra at natural abundance could be acquired. ..
  13. Grimm I, Saffian D, Girzalsky W, Erdmann R. Nucleotide-dependent assembly of the peroxisomal receptor export complex. Sci Rep. 2016;6:19838 pubmed publisher
    ..The data indicate that receptor release in peroxisomal protein import is associated with a nucleotide-depending Pex1/6p-cycle of Pex15p-binding and release. ..
  14. Schumacher D, Bergeler S, Harms A, Vonck J, Huneke Vogt S, Frey E, et al. The PomXYZ Proteins Self-Organize on the Bacterial Nucleoid to Stimulate Cell Division. Dev Cell. 2017;41:299-314.e13 pubmed publisher
    ..At midcell, fluxes equalize resulting in constrained motion. Flux-based mechanisms may represent a general paradigm for positioning of macromolecular structures in bacteria. ..
  15. Deibel C, Kramer S, Chakraborty T, Ebel F. EspE, a novel secreted protein of attaching and effacing bacteria, is directly translocated into infected host cells, where it appears as a tyrosine-phosphorylated 90 kDa protein. Mol Microbiol. 1998;28:463-74 pubmed
    ..EspE is enriched at the tip of these structures, suggesting its involvement in the process of actin dynamics, which is triggered during the attaching and effacing process. ..
  16. Bernhardt H, Schulz K, Zimmermann K, Knoke M. Influence of ciprofloxacin and other antimicrobial drugs on different Escherichia coli strains in continuous-flow cultures under aerobic and anaerobic conditions. J Antimicrob Chemother. 1998;42:147-52 pubmed
    ..These results were observed in long-term experiments followed for up to 7 days. We conclude that the antibiotics tested are highly effective against E. coli under unfavourable conditions simulating in-vivo situations. ..
  17. Beck K, Wu L, Brunner J, Muller M. Discrimination between SRP- and SecA/SecB-dependent substrates involves selective recognition of nascent chains by SRP and trigger factor. EMBO J. 2000;19:134-43 pubmed
  18. Williams K. Three-dimensional structure of the ion-coupled transport protein NhaA. Nature. 2000;403:112-5 pubmed
    ..The molecular organization of NhaA represents a new membrane-protein structural motif and offers the first insights into the architecture of an ion-coupled transport protein. ..
  19. Heermann R, Altendorf K, Jung K. The hydrophilic N-terminal domain complements the membrane-anchored C-terminal domain of the sensor kinase KdpD of Escherichia coli. J Biol Chem. 2000;275:17080-5 pubmed
    ..These results highlight the importance of the N-terminal domain for the function of KdpD and provide evidence for an interaction of this domain and the transmitter domain of the sensor kinase. ..
  20. Herz S, Eberhardt S, Bacher A. Biosynthesis of riboflavin in plants. The ribA gene of Arabidopsis thaliana specifies a bifunctional GTP cyclohydrolase II/3,4-dihydroxy-2-butanone 4-phosphate synthase. Phytochemistry. 2000;53:723-31 pubmed
    ..The N-terminal segment is not required for catalytic activity and is likely to serve as signal sequence for import into chloroplasts. ..
  21. Grauschopf U, Lilie H, Honold K, Wozny M, Reusch D, Esswein A, et al. The N-terminal fragment of human parathyroid hormone receptor 1 constitutes a hormone binding domain and reveals a distinct disulfide pattern. Biochemistry. 2000;39:8878-87 pubmed
    ..These results demonstrate that the extracellular N-terminal domain of the parathyroid hormone receptor (PTHR1) possesses a well-defined, stable conformation, which shows a significant ligand binding activity. ..
  22. Schulte R, Grassl G, Preger S, Fessele S, Jacobi C, Schaller M, et al. Yersinia enterocolitica invasin protein triggers IL-8 production in epithelial cells via activation of Rel p65-p65 homodimers. FASEB J. 2000;14:1471-84 pubmed
    ..Together, these data elucidate the possible early pathomechanisms operating in Yersinia infection and may have implications for the design of novel therapeutics directed against this enteropathogen. ..
  23. Hoffmann B, Schütze H, Mettenleiter T. Determination of the complete genomic sequence and analysis of the gene products of the virus of Spring Viremia of Carp, a fish rhabdovirus. Virus Res. 2002;84:89-100 pubmed
    ..Furthermore, monospecific antisera against the distinct viral proteins were generated. Comparison of genome and protein confirm the assignment of SVCV to the genus Vesiculovirus. ..
  24. Pfeiffer J, Guhl J, Waidner B, Kist M, Bereswill S. Magnesium uptake by CorA is essential for viability of the gastric pathogen Helicobacter pylori. Infect Immun. 2002;70:3930-4 pubmed
    ..pylori. The drastic Mg(2+) requirement (20 mM) of H. pylori corA mutants indicates that CorA plays a key role in the adaptation to the low-Mg(2+) conditions predominant in the gastric environment...
  25. Blokesch M, Bock A. Maturation of [NiFe]-hydrogenases in Escherichia coli: the HypC cycle. J Mol Biol. 2002;324:287-96 pubmed
    ..On the basis of the results, a cycle of HypC activity is proposed whose function is to transfer the iron atom that has been liganded at the HypC-HypD complex to the precursor of the large hydrogenase subunit...
  26. Schiefner A, Breed J, B sser L, Kneip S, Gade J, Holtmann G, et al. Cation-pi interactions as determinants for binding of the compatible solutes glycine betaine and proline betaine by the periplasmic ligand-binding protein ProX from Escherichia coli. J Biol Chem. 2004;279:5588-96 pubmed publisher
    ..The structural analysis was combined with site-directed mutagenesis of the ligand binding pocket to estimate the contributions of the tryptophan residues involved in binding...
  27. Gläser R, Harder J, Lange H, Bartels J, Christophers E, Schroder J. Antimicrobial psoriasin (S100A7) protects human skin from Escherichia coli infection. Nat Immunol. 2005;6:57-64 pubmed
    ..Zn(2+)-saturated psoriasin showed diminished antimicrobial activity, suggesting that Zn(2+) sequestration could be a possible antimicrobial mechanism. Thus, psoriasin may be key to the resistance of skin against E. coli. ..
  28. Tomic S, Johnson A, Hartl F, Etchells S. Exploring the capacity of trigger factor to function as a shield for ribosome bound polypeptide chains. FEBS Lett. 2006;580:72-6 pubmed
    ..Thus, TF is not a general shield for nascent chains. Protease protection appears to depend on a hydrophobic interaction of TF with nascent polypeptides. ..
  29. Wilharm G, Lepka D, Faber F, Hofmann J, Kerrinnes T, Skiebe E. A simple and rapid method of bacterial transformation. J Microbiol Methods. 2010;80:215-6 pubmed publisher
    ..A buffered suspension of sepiolite-an inexpensive, fibrous yet inoffensive mineral-is mixed with bacteria and transforming DNA and the mixture directly spread on selective agar. ..
  30. Rudolph B, Gebendorfer K, Buchner J, Winter J. Evolution of Escherichia coli for growth at high temperatures. J Biol Chem. 2010;285:19029-34 pubmed publisher
    ..Our data suggest that GroEL/GroES are especially required for the folding of mutated proteins generated during evolution. GroEL/GroES therefore appear as mediators of evolution of extremely heat-resistant E. coli cells. ..
  31. Stierl M, Stumpf P, Udwari D, Gueta R, Hagedorn R, Losi A, et al. Light modulation of cellular cAMP by a small bacterial photoactivated adenylyl cyclase, bPAC, of the soil bacterium Beggiatoa. J Biol Chem. 2011;286:1181-8 pubmed publisher
    ..bPAC seems a perfect optogenetic tool for light modulation of cAMP in neuronal cells and tissues and for studying cAMP-dependent processes in live animals. ..
  32. Fessler A, Kaspar H, Lindeman C, Stegemann M, Peters T, Mankertz J, et al. A proposal of interpretive criteria for cefoperazone applicable to bovine mastitis pathogens. Vet Microbiol. 2012;157:226-31 pubmed publisher
    ..coli) or 16-20mm (streptococci), and (c) resistant when their MIC is ? 8 ?g/ml and their zone diameters are ? 21 mm (staphylococci or E. coli) or ? 15 mm (streptococci). ..
  33. Donovan C, Sieger B, Kramer R, Bramkamp M. A synthetic Escherichia coli system identifies a conserved origin tethering factor in Actinobacteria. Mol Microbiol. 2012;84:105-16 pubmed publisher
    ..The interaction between ParB and DivIVA proteins was also shown for other members of the Actinobacteria phylum, including Mycobacterium tuberculosis and Streptomyces coelicolor...
  34. Seedorf U, Scheek S, Engel T, Steif C, Hinz H, Assmann G. Structure-activity studies of human sterol carrier protein 2. J Biol Chem. 1994;269:2613-8 pubmed
    ..Circular dichroism results showed that Leu20 and Asp70 may serve to stabilize the overall fold, whereas residue 104 appears to play a role in the specific lipid binding and/or transfer activity of SCP2. ..
  35. Perraud A, Rippe K, Bantscheff M, Glocker M, Lucassen M, Jung K, et al. Dimerization of signalling modules of the EvgAS and BvgAS phosphorelay systems. Biochim Biophys Acta. 2000;1478:341-54 pubmed
    ..In agreement with these data, surface plasmon resonance studies revealed a 2:1 stoichiometry in the interaction of EvgA with the immobilized EvgS HPt domain and an affinity constant of 1. 24x10(6) M(-1)...
  36. Geue L, Segura Alvarez M, Conraths F, Kuczius T, Bockemuhl J, Karch H, et al. A long-term study on the prevalence of shiga toxin-producing Escherichia coli (STEC) on four German cattle farms. Epidemiol Infect. 2002;129:173-85 pubmed
    ..Most STEC O26:H11 with the complete pattern of potential virulence markers were found in clinically healthy cattle. These animals may represent a risk factor for farmers and consumers. ..
  37. Wendrich T, Blaha G, Wilson D, Marahiel M, Nierhaus K. Dissection of the mechanism for the stringent factor RelA. Mol Cell. 2002;10:779-88 pubmed
  38. Schilling S, Hoffmann T, Manhart S, Hoffmann M, Demuth H. Glutaminyl cyclases unfold glutamyl cyclase activity under mild acid conditions. FEBS Lett. 2004;563:191-6 pubmed
    ..0 while Gln(1) conversion occurs with an optimum at pH 8.0. This unexpected finding might be of importance for deciphering the events leading to deposition of highly toxic pyroglutamyl peptides in amyloidotic diseases. ..
  39. Radestock S, Forrest L. The alternating-access mechanism of MFS transporters arises from inverted-topology repeats. J Mol Biol. 2011;407:698-715 pubmed publisher
    ..More fundamentally, our model demonstrates that inverted-topology repeats provide the foundation for the alternating-access mechanisms of MFS transporters. ..
  40. Kessler D, Leibrecht I, Knappe J. Pyruvate-formate-lyase-deactivase and acetyl-CoA reductase activities of Escherichia coli reside on a polymeric protein particle encoded by adhE. FEBS Lett. 1991;281:59-63 pubmed
    ..This revealed the identity of PFL-deactivase with the alcohol and acetaldehyde dehydrogenases of E. coli. The multienzyme represents a homopolymeric protein (approximately 40 x 96 kDa) requiring Fe2+ for all functions. ..
  41. Reuter K, Ficner R. Sequence analysis and overexpression of the Zymomonas mobilis tgt gene encoding tRNA-guanine transglycosylase: purification and biochemical characterization of the enzyme. J Bacteriol. 1995;177:5284-8 pubmed
    ..mobilis Tgt was found to be a monomer according to gel filtration. In this study, it was shown that the formation of homotrimers by the E. coli enzyme is readily reversible and is dependent on protein concentration...
  42. Ochs M, Veitinger S, Kim I, Welz D, Angerer A, Braun V. Regulation of citrate-dependent iron transport of Escherichia coli: fecR is required for transcription activation by FecI. Mol Microbiol. 1995;15:119-32 pubmed
    ..Transcription of the fec transport genes is positively regulated by FecI and FecR and negatively regulated by the Fe2(+)-Fur repressor. Transcription activation and repression may occur independently of each other. ..
  43. Textor S, Wendisch V, de Graaf A, Muller U, Linder M, Linder D, et al. Propionate oxidation in Escherichia coli: evidence for operation of a methylcitrate cycle in bacteria. Arch Microbiol. 1997;168:428-36 pubmed
    ..The 13C patterns of aspartate and glutamate are consistent with the further oxidation of pyruvate to acetyl-CoA. Oxaloacetate is predominantly generated via the glyoxylate cycle rather than by carboxylation of phosphoenolpyruvate. ..
  44. Jung H, Rübenhagen R, Tebbe S, Leifker K, Tholema N, Quick M, et al. Topology of the Na+/proline transporter of Escherichia coli. J Biol Chem. 1998;273:26400-7 pubmed
    ..The 13-helix structure is discussed as a common topological motif for all members of the Na+/solute cotransporter family. ..
  45. Knöfel T, Strater N. Mechanism of hydrolysis of phosphate esters by the dimetal center of 5'-nucleotidase based on crystal structures. J Mol Biol. 2001;309:239-54 pubmed
    ..The site 1 metal ion coordinates a water molecule which is in an ideal position for a nucleophilic attack on the phosphorus atom, assuming an in-line mechanism of phosphoryl transfer. Another water molecule bridges the two metal ions. ..
  46. Hink Schauer C, Estebanez Perpina E, Wilharm E, Fuentes Prior P, Klinkert W, Bode W, et al. The 2.2-A crystal structure of human pro-granzyme K reveals a rigid zymogen with unusual features. J Biol Chem. 2002;277:50923-33 pubmed
    ..We hypothesize that GzmK is proteolytically active only toward specific, as yet unidentified substrates, which upon approach transiently induce a functional active-site conformation. ..
  47. Steinbacher S, Kaiser J, Eisenreich W, Huber R, Bacher A, Rohdich F. Structural basis of fosmidomycin action revealed by the complex with 2-C-methyl-D-erythritol 4-phosphate synthase (IspC). Implications for the catalytic mechanism and anti-malaria drug development. J Biol Chem. 2003;278:18401-7 pubmed
    ..Both sites are connected by a spacer of three methylene groups. The substrate molecule, 1-d-deoxyxylulose 5-phosphate, can be superimposed onto fosmidomycin, explaining the stereochemical course of the reaction. ..
  48. Grininger M, Ravelli R, Heider U, Zeth K. Expression, crystallization and crystallographic analysis of DegS, a stress sensor of the bacterial periplasm. Acta Crystallogr D Biol Crystallogr. 2004;60:1429-31 pubmed
    ..The correctness of the molecular-replacement solution was verified by identifying radiation-damage-induced structural changes. ..
  49. Gruger T, Nitiss J, Maxwell A, Zechiedrich E, Heisig P, Seeber S, et al. A mutation in Escherichia coli DNA gyrase conferring quinolone resistance results in sensitivity to drugs targeting eukaryotic topoisomerase II. Antimicrob Agents Chemother. 2004;48:4495-504 pubmed
  50. Ruckert C, Koch D, Rey D, Albersmeier A, Mormann S, Pühler A, et al. Functional genomics and expression analysis of the Corynebacterium glutamicum fpr2-cysIXHDNYZ gene cluster involved in assimilatory sulphate reduction. BMC Genomics. 2005;6:121 pubmed
    ..coli and B. subtilis is used by members of this order, providing the basis for further biochemical studies. ..
  51. Böhling A, Hagge S, Roes S, Podschun R, Sahly H, Harder J, et al. Lipid-specific membrane activity of human beta-defensin-3. Biochemistry. 2006;45:5663-70 pubmed
    ..Therefore, we propose that the lipid-specificity of hBD3 as well as some other membrane-active antimicrobial peptides is important for their activity against bacteria or mammalian cells. ..
  52. Wiegand I, Geiss H, Mack D, Stürenburg E, Seifert H. Detection of extended-spectrum beta-lactamases among Enterobacteriaceae by use of semiautomated microbiology systems and manual detection procedures. J Clin Microbiol. 2007;45:1167-74 pubmed
    ..The sensitivities of the conventional test methods ranged from 93 to 94%. The double-disk synergy test showed the highest specificity and positive predictive value among all test methods, i.e., 97% and 98%, respectively. ..
  53. Aust S, Brüsselbach F, Pütz S, Hovemann B. Alternative tasks of Drosophila tan in neurotransmitter recycling versus cuticle sclerotization disclosed by kinetic properties. J Biol Chem. 2010;285:20740-7 pubmed publisher
    ..Kinetic parameters of Tan reveal characteristic differences in K(m) and k(cat) values of carcinine and beta-alanyl-dopamine cleavage, which conclusively illustrate the divergent tasks met by Tan. ..
  54. Gille C, Dreschers S, Leiber A, Lepiorz F, Krusch M, Grosse Opphoff J, et al. The CD95/CD95L pathway is involved in phagocytosis-induced cell death of monocytes and may account for sustained inflammation in neonates. Pediatr Res. 2013;73:402-8 pubmed publisher
    ..These in vitro results offer for the first time a molecular mechanism accounting for sustained inflammation seen in neonates. ..
  55. Heuveling J, Frochaux V, Ziomkowska J, Wawrzinek R, Wessig P, Herrmann A, et al. Conformational changes of the bacterial type I ATP-binding cassette importer HisQMP2 at distinct steps of the catalytic cycle. Biochim Biophys Acta. 2014;1838:106-16 pubmed publisher
    ..Together, our data suggest similar conformational changes during the transport cycle as described for the maltose ABC transporter of Escherichia coli, despite distinct structural differences between both systems. ..
  56. Harms A, Treuner Lange A, Schumacher D, S gaard Andersen L. Tracking of chromosome and replisome dynamics in Myxococcus xanthus reveals a novel chromosome arrangement. PLoS Genet. 2013;9:e1003802 pubmed publisher
    ..We conclude that M. xanthus chromosome arrangement and dynamics combine features from previously described systems with new features leading to a novel spatiotemporal arrangement pattern...
  57. Niedner A, Müller M, Moorthy B, Jansen R, Niessing D. Role of Loc1p in assembly and reorganization of nuclear ASH1 messenger ribonucleoprotein particles in yeast. Proc Natl Acad Sci U S A. 2013;110:E5049-58 pubmed publisher
    ..Together these findings suggest an ordered process of nuclear assembly and reorganization for the maturation of localizing ASH1 mRNPs. ..
  58. Kluwe L, Maeda K, Miegel A, Fujita Becker S, Maeda Y, Talbo G, et al. Rabbit skeletal muscle alpha alpha-tropomyosin expressed in baculovirus-infected insect cells possesses the authentic N-terminus structure and functions. J Muscle Res Cell Motil. 1995;16:103-10 pubmed
    ..The differentiated susceptibilities of potential ompT cleavage sites are indicative of a non-coiled-coil conformation of the N-terminus of alpha-tropomyosin. ..
  59. Wunderlich M, Glockshuber R. In vivo control of redox potential during protein folding catalyzed by bacterial protein disulfide-isomerase (DsbA). J Biol Chem. 1993;268:24547-50 pubmed
    ..The high stability of thiol reagents against air oxidation during growth of E. coli allows the investigation of oxidative protein folding in vivo under controlled, thiol-dependent redox conditions. ..
  60. Wackwitz B, Bongaerts J, Goodman S, Unden G. Growth phase-dependent regulation of nuoA-N expression in Escherichia coli K-12 by the Fis protein: upstream binding sites and bioenergetic significance. Mol Gen Genet. 1999;262:876-83 pubmed
    ..This ensures higher ATP yields under conditions where large amounts of ATP are required. ..
  61. Kosinski J, Steindorf I, Bujnicki J, Giron Monzon L, Friedhoff P. Analysis of the quaternary structure of the MutL C-terminal domain. J Mol Biol. 2005;351:895-909 pubmed
  62. Barth E, Gora K, Gebendorfer K, Settele F, Jakob U, Winter J. Interplay of cellular cAMP levels, {sigma}S activity and oxidative stress resistance in Escherichia coli. Microbiology. 2009;155:1680-9 pubmed publisher
    ..Our results demonstrate the close regulatory interplay between cellular cAMP levels, sigma(S) activity and oxidative stress resistance in E. coli. ..
  63. Infed N, Hanekop N, Driessen A, Smits S, Schmitt L. Influence of detergents on the activity of the ABC transporter LmrA. Biochim Biophys Acta. 2011;1808:2313-21 pubmed publisher
  64. Henrich E, Ma Y, Engels I, Münch D, Otten C, Schneider T, et al. Lipid Requirements for the Enzymatic Activity of MraY Translocases and in Vitro Reconstitution of the Lipid II Synthesis Pathway. J Biol Chem. 2016;291:2535-46 pubmed publisher
    ..As a proof of principle for future screening platforms, we demonstrate the inhibition of the in vitro lipid II biosynthesis with the specific inhibitors fosfomycin, feglymycin, and tunicamycin. ..
  65. Kontermann R, Bautz E. Similarity between subunit 8 of yeast RNA polymerase II (RPB8) and the second-largest subunits of eukaryotic RNA polymerases. Nucleic Acids Res. 1992;20:5231 pubmed
  66. Sawers R. Identification and molecular characterization of a transcriptional regulator from Pseudomonas aeruginosa PAO1 exhibiting structural and functional similarity to the FNR protein of Escherichia coli. Mol Microbiol. 1991;5:1469-81 pubmed
    ..These results imply that the mechanisms by which ANR and FNR regulate transcription are fundamentally similar. ..
  67. Kampfenkel K, Braun V. Topology of the ExbB protein in the cytoplasmic membrane of Escherichia coli. J Biol Chem. 1993;268:6050-7 pubmed
    ..Both proteins were lacking positive charges at the inner side of the cytoplasmic membrane which determine the orientation of transmembrane segments. ..
  68. Schwartz E, Gerischer U, Friedrich B. Transcriptional regulation of Alcaligenes eutrophus hydrogenase genes. J Bacteriol. 1998;180:3197-204 pubmed
    ..The two promoters were induced at approximately the same rates upon derepression of the hydrogenase system in diauxic cultures. The response regulator HoxA mediated low-level activation of PSH and PMBH in a heterologous system...
  69. Vonrhein C, B nisch H, Sch fer G, Schulz G. The structure of a trimeric archaeal adenylate kinase. J Mol Biol. 1998;282:167-79 pubmed publisher
    ..The conformational differences between these states resemble those of other adenylate kinases. Because of sequence homology, the structure presented provides a good model for the methanococcal adenylate kinases...
  70. Orth P, Schnappinger D, Hillen W, Saenger W, Hinrichs W. Structural basis of gene regulation by the tetracycline inducible Tet repressor-operator system. Nat Struct Biol. 2000;7:215-9 pubmed publisher
    ..This forces a pendulum-like motion of helix a4, which increases the separation of the attached DNA binding domains by 3 A, abolishing the affinity of TetR for its operator DNA...
  71. Freiberg C, Brunner N, Schiffer G, Lampe T, Pohlmann J, Brands M, et al. Identification and characterization of the first class of potent bacterial acetyl-CoA carboxylase inhibitors with antibacterial activity. J Biol Chem. 2004;279:26066-73 pubmed
  72. Zuber D, Krause R, Venturi M, Padan E, Bamberg E, Fendler K. Kinetics of charge translocation in the passive downhill uptake mode of the Na+/H+ antiporter NhaA of Escherichia coli. Biochim Biophys Acta. 2005;1709:240-50 pubmed
    ..3 mM for Li+ at basic pH and 180 mM for Na+ and 50 mM for Li+ at neutral pH. The data demonstrate that in the passive downhill uptake mode pH regulation of the carrier affects both apparent Km as well as turnover (Vmax). ..
  73. Assenmacher N, Wenig K, Lammens A, Hopfner K. Structural basis for transcription-coupled repair: the N terminus of Mfd resembles UvrB with degenerate ATPase motifs. J Mol Biol. 2006;355:675-83 pubmed
    ..Thus, our results suggest that Mfd might form a UvrA recruitment factor at stalled transcription complexes that architecturally but not catalytically resembles UvrB. ..
  74. Schlicht M, Berens C, Daam J, Hillen W. Random insertion of a TetR-inducing peptide tag into Escherichia coli proteins allows analysis of protein levels by induction of reporter gene expression. Appl Environ Microbiol. 2006;72:5637-42 pubmed
    ..The expression patterns of tagged Escherichia coli genes fully agree with published data from transcriptional fusions or microarrays, validating the Tip tag approach. ..
  75. Hoffmann D, Wildner O. Efficient generation of double heterologous promoter controlled oncolytic adenovirus vectors by a single homologous recombination step in Escherichia coli. BMC Biotechnol. 2006;6:36 pubmed
    ..The generated viruses showed preferential replication in tumor cells and in a subcutaneous HT-29 colon cancer xenograft model the viruses demonstrated significant oncolytic activity comparable with dl327. ..
  76. Fuchs W, Klupp B, Granzow H, Leege T, Mettenleiter T. Characterization of pseudorabies virus (PrV) cleavage-encapsidation proteins and functional complementation of PrV pUL32 by the homologous protein of herpes simplex virus type 1. J Virol. 2009;83:3930-43 pubmed publisher
    ..However, trans-complementation of UL32-negative HSV-1 by the PrV protein was not observed. ..
  77. Botella L, Lindley N, Eggeling L. Formation and metabolism of methylmalonyl coenzyme A in Corynebacterium glutamicum. J Bacteriol. 2009;191:2899-901 pubmed publisher
    ..The physiological context of the mutase present in members of the suborder Corynebacterineae is discussed. ..
  78. Schicklberger M, Bücking C, Schuetz B, Heide H, Gescher J. Involvement of the Shewanella oneidensis decaheme cytochrome MtrA in the periplasmic stability of the beta-barrel protein MtrB. Appl Environ Microbiol. 2011;77:1520-3 pubmed publisher
    ..Quantitative PCR, heterologous gene expression, and mutant studies indicated that MtrA is required for periplasmic stability of MtrB. DegP depletion compensated for this MtrA dependence...
  79. Mondéjar L, Lupas A, Schultz A, Schultz J. HAMP domain-mediated signal transduction probed with a mycobacterial adenylyl cyclase as a reporter. J Biol Chem. 2012;287:1022-31 pubmed publisher
    ..Finally, we present data indicating that the connector, which links the ?-helices of HAMP domains, plays an important structural role in HAMP function. ..
  80. Zendah I, Riaz N, Nasr H, Frauendorf H, Schüffler A, Raies A, et al. Chromophenazines from the terrestrial Streptomyces sp. Ank 315. J Nat Prod. 2012;75:2-8 pubmed publisher
    ..Ank 315. The structures of the new compounds were established on the basis of spectroscopic data, 1D NOE, 2D NMR, and ESIMS measurements and comparison with literature values. ..
  81. Endesfelder U, Finan K, Holden S, Cook P, Kapanidis A, Heilemann M. Multiscale spatial organization of RNA polymerase in Escherichia coli. Biophys J. 2013;105:172-81 pubmed publisher
    ..Apart from their importance for bacterial transcription, our studies pave the way for molecular-level analysis of several cellular processes at the nanometer scale. ..
  82. Hesterkamp T, Bukau B. The Escherichia coli trigger factor. FEBS Lett. 1996;389:32-4 pubmed
    ..These findings suggest roles for trigger factor in various folding processes of secretory as well as cytosolic proteins. ..
  83. Kaiser M, Sawers G. Overlapping promoters modulate Fnr- and ArcA-dependent anaerobic transcriptional activation of the focApfl operon in Escherichia coli. Microbiology. 1997;143 ( Pt 3):775-83 pubmed
    ..Taken together, these results indicate that the P6A promoter moderates the Fnr-dependent activation of P6 through competition for RNA polymerase binding. ..
  84. Hinderlich S, Berger M, Schwarzkopf M, Effertz K, Reutter W. Molecular cloning and characterization of murine and human N-acetylglucosamine kinase. Eur J Biochem. 2000;267:3301-8 pubmed
    ..mRNA of the human enzyme was expressed in all investigated human tissues, as well as in cancer cell lines. In both the tissues and the cancer cell lines, the human mRNA was 1.35 kb in size. ..
  85. Jossek R, Bongaerts J, Sprenger G. Characterization of a new feedback-resistant 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase AroF of Escherichia coli. FEMS Microbiol Lett. 2001;202:145-8 pubmed
    ..These results strongly suggest that the N-terminus of AroF is involved in the molecular interactions occurring in the feedback-inhibition mechanism. ..
  86. Kaetzke A, Eschrich K. Simultaneous determination of different DNA sequences by mass spectrometric evaluation of Sanger sequencing reactions. Nucleic Acids Res. 2002;30:e117 pubmed
    ..Short sequences with a length between 21 and 25 nt were sufficient to reflect the heterogeneity of the 16S rDNA genes in E.coli and the existence of three isoenzymes of PFK-1 in rat brain. ..
  87. Zaitseva J, Holland I, Schmitt L. The role of CAPS buffer in expanding the crystallization space of the nucleotide-binding domain of the ABC transporter haemolysin B from Escherichia coli. Acta Crystallogr D Biol Crystallogr. 2004;60:1076-84 pubmed
    ..Although the focus of these investigations was placed on HlyB-NBD, the strategy described here can be directly transferred to other proteins that display instability in solution...
  88. Schachtsiek M, Hammes W, Hertel C. Characterization of Lactobacillus coryniformis DSM 20001T surface protein Cpf mediating coaggregation with and aggregation among pathogens. Appl Environ Microbiol. 2004;70:7078-85 pubmed
    ..These results together with those of the amino acid sequence analysis suggest that Cpf is a novel surface protein of L. coryniformis that mediates co/aggregation of some pathogens. ..
  89. Lorch M, Faham S, Kaiser C, Weber I, Mason A, Bowie J, et al. How to prepare membrane proteins for solid-state NMR: A case study on the alpha-helical integral membrane protein diacylglycerol kinase from E. coli. Chembiochem. 2005;6:1693-700 pubmed
    ..However, reconstitution is the method of choice for biophysical studies by solid-state NMR. In addition, we discuss the identification of lipids bound to membrane-protein crystals by 31P-MAS NMR. ..
  90. Schulmeister S, Ruttorf M, Thiem S, Kentner D, Lebiedz D, Sourjik V. Protein exchange dynamics at chemoreceptor clusters in Escherichia coli. Proc Natl Acad Sci U S A. 2008;105:6403-8 pubmed publisher
    ..Our results indicate that the rates of protein turnover at the cluster have evolved to ensure optimal performance of the chemotaxis pathway. ..
  91. Mock H, Trainotti L, Kruse E, Grimm B. Isolation, sequencing and expression of cDNA sequences encoding uroporphyrinogen decarboxylase from tobacco and barley. Plant Mol Biol. 1995;28:245-56 pubmed
    ..The amount of RNA and protein increased during illumination. Maximum UROD-RNA levels were detected in the basal segments relative to the top of the leaf. ..
  92. Decker K, Krauel V, Meesmann A, Heller K. Lytic conversion of Escherichia coli by bacteriophage T5: blocking of the FhuA receptor protein by a lipoprotein expressed early during infection. Mol Microbiol. 1994;12:321-32 pubmed
    ..Since Llp-mediated T5 resistance has several aspects in common with the phenomenon of lysogenic conversion, we suggest that it should be called lytic conversion. ..