Experts and Doctors on entomopoxvirinae in Japan
Mitsuhashi W, Saito H, Sato M. Complete nucleotide sequence of the fusolin gene of an entomopoxvirus in the cupreous chafer, Anomala cuprea Hope (Coleoptera: Scarabaeidae). Insect Biochem Mol Biol. 1997;27:869-76 pubmed
..Furthermore, the last 330 bp region of the ORF revealed a low homology with that of MmEPV...
Mitsuhashi W, Saito H, Sato M, Nakashima N, Noda H. Complete nucleotide sequence of spheroidin gene of Anomala cuprea entomopoxvirus. Virus Res. 1998;55:61-9 pubmed
..The number of cysteine residues and N-glycosylation sites also depended on the difference in genera (A or B). Thus, the spheroidin gene of EPVs was well conserved within the same genus...
Mitsuhashi W, Sato M, Hirai Y. Involvement of spindles of an entomopoxvirus (EPV) in infectivity of the EPVs to their host insect. Arch Virol. 2000;145:1465-71 pubmed
..cuprea larvae. A significant increase in infectivity was induced by the addition of spindles to the spheroids, strongly suggesting that the spindles play an important role in the infection of the host insect...
Furuta Y, Mitsuhashi W, Kobayashi J, Hayasaka S, Imanishi S, Chinzei Y, et al
. Peroral infectivity of non-occluded viruses of Bombyx mori nucleopolyhedrovirus and polyhedrin-negative recombinant baculoviruses to silkworm larvae is drastically enhanced when administered with Anomala cuprea entomopoxvirus spindles. J Gen Virol. 2001;82:307-12 pubmed
..In contrast, spheroids (proteinaceous structures containing AcEPV virions) did not enhance the peroral infectivity of either NOVs or the recombinant BmNPV in silkworm larvae...
Mitsuhashi W. Further evidence that spindles of an entomopoxvirus enhance its infectivity in a host insect. J Invertebr Pathol. 2002;79:59-61 pubmed
Mitsuhashi W, Kawakita H, Murakami R, Takemoto Y, Saiki T, Miyamoto K, et al
. Spindles of an entomopoxvirus facilitate its infection of the host insect by disrupting the peritrophic membrane. J Virol. 2007;81:4235-43 pubmed
Takemoto Y, Mitsuhashi W, Murakami R, Konishi H, Miyamoto K. The N-terminal region of an entomopoxvirus fusolin is essential for the enhancement of peroral infection, whereas the C-terminal region is eliminated in digestive juice. J Virol. 2008;82:12406-15 pubmed publisher
..These results suggest that the enhancement of infectivity involves more than the tolerance to digestive juice and chitin-binding ability. ..
Mitsuhashi W, Miyamoto K, Wada S. The complete genome sequence of the Alphaentomopoxvirus Anomala cuprea entomopoxvirus, including its terminal hairpin loop sequences, suggests a potentially unique mode of apoptosis inhibition and mode of DNA replication. Virology. 2014;452-453:95-116 pubmed publisher
..Furthermore, an ORF in the genome contains a serpin domain that was previously found in vertebrate poxviruses for apoptosis inhibition but not in insect viruses...