Experts and Doctors on electron transport complex i in Finland


Locale: Finland
Topic: electron transport complex i

Top Publications

  1. Blokhina O, Fagerstedt K. Reactive oxygen species and nitric oxide in plant mitochondria: origin and redundant regulatory systems. Physiol Plant. 2010;138:447-62 pubmed publisher
    ..Mitochondrial ROS and NO production is tightly controlled by multiple redundant systems providing the regulatory mechanism for redox homeostasis and specific ROS/NO signaling. ..
  2. Sanz A, Soikkeli M, Portero Otin M, Wilson A, Kemppainen E, McIlroy G, et al. Expression of the yeast NADH dehydrogenase Ndi1 in Drosophila confers increased lifespan independently of dietary restriction. Proc Natl Acad Sci U S A. 2010;107:9105-10 pubmed publisher
    ..Our results support a central role of mitochondrial oxidative phosphorylation complex I in influencing longevity via oxidative stress, independently of pathways connected to nutrition and growth signaling. ..
  3. Euro L, Belevich G, Bloch D, Verkhovsky M, Wikstrom M, Verkhovskaya M. The role of the invariant glutamate 95 in the catalytic site of Complex I from Escherichia coli. Biochim Biophys Acta. 2009;1787:68-73 pubmed publisher
  4. Belevich N, von Ballmoos C, Verkhovskaya M. Activation of Proton Translocation by Respiratory Complex I. Biochemistry. 2017;56:5691-5697 pubmed publisher
    ..We conclude that Complex I undergoes a conversion from a decoupled state to a coupled state upon activation. The possible origins and importance of the observed phenomenon are discussed...
  5. Scialò F, Sriram A, Fernández Ayala D, Gubina N, Lõhmus M, Nelson G, et al. Mitochondrial ROS Produced via Reverse Electron Transport Extend Animal Lifespan. Cell Metab. 2016;23:725-34 pubmed publisher
    ..These results illustrate that the source of a ROS signal is vital in determining its effects on cellular physiology and establish that manipulation of ubiquinone redox state is a valid strategy to delay aging. ..
  6. Wikstrom M, Hummer G. Stoichiometry of proton translocation by respiratory complex I and its mechanistic implications. Proc Natl Acad Sci U S A. 2012;109:4431-6 pubmed publisher
    ..In a rough mechanistic model, we suggest a concerted proton translocation mechanism in the three homologous and tightly packed antiporter-like subunits L, M, and N of the proton-translocating membrane domain of complex I...
  7. Pätsi J, Maliniemi P, Pakanen S, Hinttala R, Uusimaa J, Majamaa K, et al. LHON/MELAS overlap mutation in ND1 subunit of mitochondrial complex I affects ubiquinone binding as revealed by modeling in Escherichia coli NDH-1. Biochim Biophys Acta. 2012;1817:312-8 pubmed publisher
    ..In all, m.3376G>A perturbs ubiquinone binding, a phenomenon found in LHON, and decreases the activity of fully assembled complex I as in MELAS. ..
  8. Verkhovskaya M, Knuuti J, Wikstrom M. Role of Ca(2+) in structure and function of Complex I from Escherichia coli. Biochim Biophys Acta. 2011;1807:36-41 pubmed publisher
    ..coli Complex I, analogous to Nqo15 in the T. thermophilus enzyme, or another component of the native membrane that affects the affinity and/or accessibility of the Ca(2+) binding site...
  9. Euro L, Belevich G, Wikstrom M, Verkhovskaya M. High affinity cation-binding sites in Complex I from Escherichia coli. Biochim Biophys Acta. 2009;1787:1024-8 pubmed publisher
    ..K(+) and La(3+) do not occupy the same site. Possible localization of these metal-binding sites and their implication in catalysis are discussed. ..

More Information


  1. Finel M, Majander A. Studies on the proton-translocating NADH:ubiquinone oxidoreductases of mitochondria and Escherichia coli using the inhibitor 1,10-phenanthroline. FEBS Lett. 1994;339:142-6 pubmed
    ..EPR spectroscopy of membranous E. coli NDH1 shows that two slow- and one fast-relaxing Fe-S clusters become detectable upon reduction by NADH in the presence of OP. However, none of them resembles the mitochondrial cluster 2. ..
  2. Euro L, Belevich G, Verkhovsky M, Wikstrom M, Verkhovskaya M. Conserved lysine residues of the membrane subunit NuoM are involved in energy conversion by the proton-pumping NADH:ubiquinone oxidoreductase (Complex I). Biochim Biophys Acta. 2008;1777:1166-72 pubmed publisher
    ..A tentative principle of proton translocation by Complex I is suggested based on electrostatic interactions of lysines in the membrane subunits. ..
  3. Pätsi J, Kervinen M, Finel M, Hassinen I. Leber hereditary optic neuropathy mutations in the ND6 subunit of mitochondrial complex I affect ubiquinone reduction kinetics in a bacterial model of the enzyme. Biochem J. 2008;409:129-37 pubmed
    ..How these changes in the enzyme's catalytic properties contribute to LHON pathogenesis is discussed. ..
  4. Belevich G, Euro L, Wikstrom M, Verkhovskaya M. Role of the conserved arginine 274 and histidine 224 and 228 residues in the NuoCD subunit of complex I from Escherichia coli. Biochemistry. 2007;46:526-33 pubmed
  5. Kervinen M, Hinttala R, Helander H, Kurki S, Uusimaa J, Finel M, et al. The MELAS mutations 3946 and 3949 perturb the critical structure in a conserved loop of the ND1 subunit of mitochondrial complex I. Hum Mol Genet. 2006;15:2543-52 pubmed
    ..The results provide new information on the function of the region affected by the MELAS mutations 3946 and 3949 that is not obtainable from patient samples or current eukaryote models. ..
  6. Sinegina L, Wikstrom M, Verkhovsky M, Verkhovskaya M. Activation of isolated NADH:ubiquinone reductase I (complex I) from Escherichia coli by detergent and phospholipids. Recovery of ubiquinone reductase activity and changes in EPR signals of iron-sulfur clusters. Biochemistry. 2005;44:8500-6 pubmed
    ..895, 1.904, 2.05, which corresponds to the parameters reported for the N2 cluster. This data indicates conformational rearrangements of catalytic importance in complex I upon binding of phospholipids. ..
  7. Lavikainen A, Lehtinen M, Meri T, Hirvelä Koski V, Meri S. Molecular genetic characterization of the Fennoscandian cervid strain, a new genotypic group (G10) of Echinococcus granulosus. Parasitology. 2003;127:207-15 pubmed
    ..granulosus form represents a distinct, previously undescribed genotype of E. granulosus. The novel genotype is hereby named as the Fennoscandian cervid strain (G10). ..
  8. Zickermann V, Barquera B, Wikstrom M, Finel M. Analysis of the pathogenic human mitochondrial mutation ND1/3460, and mutations of strictly conserved residues in its vicinity, using the bacterium Paracoccus denitrificans. Biochemistry. 1998;37:11792-6 pubmed
    ..The results suggest that the mutated residues play an important role in ubiquinone reduction by Complex I...