Genomes and Genes
Experts and Doctors on drosophila melanogaster in Hungary
Topic: drosophila melanogaster
- Pardi N, Vámos E, Ujfaludi Z, Komonyi O, Bodai L, Boros I. In vivo effects of abolishing the single canonical sumoylation site in the C-terminal region of Drosophila p53. Acta Biol Hung. 2011;62:397-412 pubmed publisher..From these data we conclude that under normal developmental conditions the loss of SUMO modification at K302 does not affect Dmp53 function significantly. ..
- Villanyi Z, Gaspar I, Szikora S, Puskas L, Szabad J. The involvement of Importin-? and peroxiredoxin-6005 in mitochondrial biogenesis. Mech Dev. 2011;128:191-9 pubmed publisher..It appears that peroxiredoxin-6005 is engaged in mitochondrial biogenesis possibly as a component of redox-signaling. ..
- BarkÃ³ S, Bugyi B, Carlier M, Gombos R, Matusek T, MihÃ¡ly J, et al. Characterization of the biochemical properties and biological function of the formin homology domains of Drosophila DAAM. J Biol Chem. 2010;285:13154-69 pubmed publisher..Both DAAM fragments also bind to the sides of the actin filaments and induce actin bundling. These observations show that the D. melanogaster DAAM formin represents an extreme class of barbed end regulators gated by profilin. ..
- Kovacs L, Alexa A, Klement E, Kókai E, Tantos A, Gogl G, et al. Regulation of calpain B from Drosophila melanogaster by phosphorylation. FEBS J. 2009;276:4959-72 pubmed publisher..The data obtained suggest that the activation of the ERK pathway by extracellular signals results in the phosphorylation and activation of calpain B in fruit flies. ..
- Juhász I, Villanyi Z, Tombácz I, Boros I. High Fcp1 phosphatase activity contributes to setting an intense transcription rate required in Drosophila nurse and follicular cells for egg production. Gene. 2012;509:60-7 pubmed publisher..Fcp1 over-expression results in increased expression of microtubules in nurse cells. Our results show that tightly controlled high level Fcp1 expression in the nurse cells of Drosophila ovaries is required for proper egg maturation. ..
- Abrusan G, Szilagyi A, Zhang Y, Papp B. Turning gold into 'junk': transposable elements utilize central proteins of cellular networks. Nucleic Acids Res. 2013;41:3190-200 pubmed publisher..We propose that TEs face a similar evolutionary pressure to evolve proteins with high interacting capacities and take some of the necessary protein domains directly from their hosts. ..
- TakÃ¡ts S, Nagy P, Varga Ã, Pircs K, KÃ¡rpÃ¡ti M, Varga K, et al. Autophagosomal Syntaxin17-dependent lysosomal degradation maintains neuronal function in Drosophila. J Cell Biol. 2013;201:531-9 pubmed publisher..Our findings reveal the molecular mechanism underlying autophagosomal fusion events and show that lysosomal degradation and recycling of sequestered autophagosome content is crucial to maintain proper functioning of the nervous system. ..
- Venkei Z, Gaspar I, Toth G, Szabad J. alpha4-Tubulin is involved in rapid formation of long microtubules to push apart the daughter centrosomes during earlyx Drosophila embryogenesis. J Cell Sci. 2006;119:3238-48 pubmed..Similarly, in the neuroblasts, ectopically expressed E82K-alpha4-tubulin becomes incorporated into the microtubules that grow sufficiently long and function normally. ..
- Komonyi O, Papai G, Enunlu I, Muratoglu S, Pankotai T, Kopitova D, et al. DTL, the Drosophila homolog of PIMT/Tgs1 nuclear receptor coactivator-interacting protein/RNA methyltransferase, has an essential role in development. J Biol Chem. 2005;280:12397-404 pubmed..Interestingly, two transgenes bearing a mutation in uORF or dORF in trans can result in complete rescue. ..
- Pecsenye K, Komlósi I, Saura A. Heritabilities and additive genetic variances of the activities of some enzymes in Drosophila melanogaster populations living in different habitats. Heredity (Edinb). 2004;93:215-21 pubmed..The heritability values were rather high and they had a considerable variation both between the habitats and across the enzymes. ..
- Adam G, Gausz J, Noselli S, Kurucz E, Ando I, Udvardy A. Tissue- and developmental stage-specific changes in the subcellular localization of the 26S proteasome in the ovary of Drosophila melanogaster. Gene Expr Patterns. 2004;4:329-33 pubmed..These results demonstrate that the subcellular distribution of the 26S proteasome in higher eukaryotes is strictly tissue- and developmental stage-specific. ..
- Bekesi A, Zagyva I, Hunyadi Gulyas E, Pongrácz V, Kovári J, Nagy A, et al. Developmental regulation of dUTPase in Drosophila melanogaster. J Biol Chem. 2004;279:22362-70 pubmed..Using independent approaches, the existence of such macromolecular partners was verified. ..
- Spadoni C, Farkas A, Sinka R, Tompa P, Friedrich P. Molecular cloning and RNA expression of a novel Drosophila calpain, Calpain C. Biochem Biophys Res Commun. 2003;303:343-9 pubmed..Moreover, we analysed Calpain C RNA expression during Drosophila development by RT-PCR and RNA in situ hybridization, which revealed strong expression in the salivary glands. ..
- Lipinszki Z, Klement E, Hunyadi Gulyas E, Medzihradszky K, Márkus R, Pal M, et al. A novel interplay between the ubiquitin–proteasome system and serine proteases during Drosophila development. Biochem J. 2013;454:571-83 pubmed..It may prevent the accumulation of polyubiquitylated proteins in larvae contrary to the low polyubiquitin receptor concentration. ..
- TÃ³th M, Majoros A, Vig A, Migh E, Nyitrai M, MihÃ¡ly J, et al. Biochemical Activities of the Wiskott-Aldrich Syndrome Homology Region 2 Domains of Sarcomere Length Short (SALS) Protein. J Biol Chem. 2016;291:667-80 pubmed publisher..Consequently, the interactions of the WH2 domains of SALS with actin must be tuned in the cellular context by other modules of the protein and/or sarcomeric components for its proper functioning. ..
- Timinszky G, Tirián L, Nagy F, Toth G, Perczel A, Kiss László Z, et al. The importin-beta P446L dominant-negative mutant protein loses RanGTP binding ability and blocks the formation of intact nuclear envelope. J Cell Sci. 2002;115:1675-87 pubmed..However, it interferes with nuclear envelope formation during mitosis-to-interphase transition, revealing a novel function of importin-beta. ..
- Tirián L, Puro J, Erdelyi M, Boros I, Papp B, Lippai M, et al. The Ketel(D) dominant-negative mutations identify maternal function of the Drosophila importin-beta gene required for cleavage nuclei formation. Genetics. 2000;156:1901-12 pubmed..The ketel(r) homozygous clones are fully viable in the follicle epithelium in wings and tergites. The Ketel gene is not expressed in most larval tissues, as revealed by the expression pattern of a Ketel promoter-lacZ reporter gene. ..
- Jekely G, Friedrich P. Characterization of two recombinant Drosophila calpains. CALPA and a novel homolog, CALPB. J Biol Chem. 1999;274:23893-900 pubmed..The analysis of the mechanism of activation in Drosophila calpains seems to corroborate the autolysis model of calpain activation. ..
- Torok T, Gorjanacz M, Bryant P, Kiss I. Prod is a novel DNA-binding protein that binds to the 1.686 g/cm(3) 10 bp satellite repeat of Drosophila melanogaster. Nucleic Acids Res. 2000;28:3551-7 pubmed
- LÅ‘rincz P, TÃ³th S, BenkÅ‘ P, Lakatos Z, Boda A, Glatz G, et al. Rab2 promotes autophagic and endocytic lysosomal degradation. J Cell Biol. 2017;216:1937-1947 pubmed publisher..We also demonstrate that RAB2A is required for autophagosome clearance in human breast cancer cells. In conclusion, we identify Rab2 as a key factor for autophagic and endocytic cargo delivery to and degradation in lysosomes. ..
- Muha V, Zagyva I, Venkei Z, Szabad J, Vertessy B. Nuclear localization signal-dependent and -independent movements of Drosophila melanogaster dUTPase isoforms during nuclear cleavage. Biochem Biophys Res Commun. 2009;381:271-5 pubmed publisher..The observed dynamic localization character showed strict timing to the nuclear cleavage phases and explained how both isoforms can be present within the nuclear microenvironment, although at different stages of cell cycle. ..
- Szalontai T, Gaspar I, Belecz I, Kerekes I, Erdelyi M, Boros I, et al. HorkaD, a chromosome instability-causing mutation in Drosophila, is a dominant-negative allele of Lodestar. Genetics. 2009;181:367-77 pubmed publisher..The Horka(D) mutation results in replacement of Ala777 with Thr, which we suggest causes chromosome instability by increasing the affinity of Lodestar for chromatin. ..
- Ciurciu A, Komonyi O, Boros I. Loss of ATAC-specific acetylation of histone H4 at Lys12 reduces binding of JIL-1 to chromatin and phosphorylation of histone H3 at Ser10. J Cell Sci. 2008;121:3366-72 pubmed publisher..We propose that a reduced level of histone H4 acetylated at Lys12 by ATAC attenuates histone H3 phosphorylation at Ser10 by JIL-1 owing to reduced binding of JIL-1 to hypoacetylated chromatin. ..
- Sipos L, Kozma G, Molnár E, Bender W. In situ dissection of a Polycomb response element in Drosophila melanogaster. Proc Natl Acad Sci U S A. 2007;104:12416-21 pubmed..These deletions resulted in neither loss of Ubx expression nor loss-of-function phenotypes. Thus, the 3-kb region including the PRE is required for repression, but not for activation, of Ubx. ..
- Ciurciu A, Komonyi O, Pankotai T, Boros I. The Drosophila histone acetyltransferase Gcn5 and transcriptional adaptor Ada2a are involved in nucleosomal histone H4 acetylation. Mol Cell Biol. 2006;26:9413-23 pubmed..Our data suggest dAda2a involvement in both uniformly distributed H4 acetylation and gene-specific transcription regulation. ..
- Vilmos P, Nagy I, Kurucz E, Hultmark D, Gateff E, Ando I. A rapid rosetting method for separation of hemocyte sub-populations of Drosophila melanogaster. Dev Comp Immunol. 2004;28:555-63 pubmed..The method can be used for functional and molecular characterisation of hemocyte sub-populations. The procedure is sensitive, reproducible and easy to perform. ..
- Szlanka T, Haracska L, Kiss I, Deak P, Kurucz E, Ando I, et al. Deletion of proteasomal subunit S5a/Rpn10/p54 causes lethality, multiple mitotic defects and overexpression of proteasomal genes in Drosophila melanogaster. J Cell Sci. 2003;116:1023-33 pubmed
- Muratoglu S, Georgieva S, Papai G, Scheer E, Enunlu I, Komonyi O, et al. Two different Drosophila ADA2 homologues are present in distinct GCN5 histone acetyltransferase-containing complexes. Mol Cell Biol. 2003;23:306-21 pubmed..Furthermore, in vivo the two dADA2 proteins showed different localizations on polytene X chromosomes. These results, taken together, suggest that the two Drosophila ADA2 homologues are present in distinct GCN5-containing HAT complexes. ..
- Pecsenye K, Saura A. Structure of variation in enzyme activity in natural Drosophila melanogaster populations. Hereditas. 2002;136:75-83 pubmed..Larval activities of the investigated enzymes showed much stronger correlation than adult activities. The correlation pattern in the adults differed greatly between the two populations. ..
- Lippai M, Tirián L, Boros I, Mihaly J, Erdelyi M, Belecz I, et al. The Ketel gene encodes a Drosophila homologue of importin-beta. Genetics. 2000;156:1889-900 pubmed..The Ketel gene appears to be ubiquitously expressed in embryonic cells. Western blot analysis revealed that the Ketel gene is not expressed in several larval cell types of late third instar larvae. ..