Experts and Doctors on deoxyribonucleases in Germany


Locale: Germany
Topic: deoxyribonucleases

Top Publications

  1. Fahmueller Y, Nagel D, Hoffmann R, Tatsch K, Jakobs T, Stieber P, et al. Immunogenic cell death biomarkers HMGB1, RAGE, and DNAse indicate response to radioembolization therapy and prognosis in colorectal cancer patients. Int J Cancer. 2013;132:2349-58 pubmed publisher
    ..HMGB1 is a valuable serum biomarker for early estimation of therapy response and prognosis in colorectal cancer patients with liver metastases undergoing RE therapy. ..
  2. Ballmann M, von der Hardt H. Hypertonic saline and recombinant human DNase: a randomised cross-over pilot study in patients with cystic fibrosis. J Cyst Fibros. 2002;1:35-7 pubmed
  3. Scholz S, Korn C, Bujnicki J, Gimadutdinow O, Pingoud A, Meiss G. Experimental evidence for a beta beta alpha-Me-finger nuclease motif to represent the active site of the caspase-activated DNase. Biochemistry. 2003;42:9288-94 pubmed
    ..Our findings provide a first insight into the likely active-site structure and catalytic mechanism of a nuclease involved in the degradation of chromosomal DNA during programmed cell death. ..
  4. Claros M, Claros Z, Hecht D, Citron D, Goldstein E, Silva J, et al. Characterization of the Bacteroides fragilis pathogenicity island in human blood culture isolates. Anaerobe. 2006;12:17-22 pubmed
    ..fragilis isolates from other clinical sources were 10% pattern I, 47% pattern II and 43% pattern III. This suggests that the pathogenicity island and the flanking elements may be general virulence factors of B. fragilis. ..
  5. Mussolino C, Alzubi J, Fine E, Morbitzer R, Cradick T, Lahaye T, et al. TALENs facilitate targeted genome editing in human cells with high specificity and low cytotoxicity. Nucleic Acids Res. 2014;42:6762-73 pubmed publisher
    ..In conclusion, our results link nuclease-associated toxicity to off-target cleavage activity and corroborate TALENs as a highly specific platform for future clinical translation. ..
  6. Böttcher R, Hollmann M, Merk K, Nitschko V, Obermaier C, Philippou Massier J, et al. Efficient chromosomal gene modification with CRISPR/cas9 and PCR-based homologous recombination donors in cultured Drosophila cells. Nucleic Acids Res. 2014;42:e89 pubmed publisher
    ..The system can be used for epitope tagging or reporter gene knock-ins in an experimental setup that can in principle be fully automated. ..
  7. Klaschik S, Lehmann L, Raadts A, Hoeft A, Stuber F. Comparison of different decontamination methods for reagents to detect low concentrations of bacterial 16S DNA by real-time-PCR. Mol Biotechnol. 2002;22:231-42 pubmed
    ..However, decontamination with DNAse may help, together with the use of highly purified PCR reagents, in detecting small amounts of bacterial DNA in clinical specimens. ..
  8. Reinke H, Hörz W. Anatomy of a hypersensitive site. Biochim Biophys Acta. 2004;1677:24-9 pubmed
    ..The implications for generating a hypersensitive region in chromatin are discussed. ..
  9. Miertzschke M, Greiner Stöffele T. The xthA gene product of Archaeoglobus fulgidus is an unspecific DNase. Eur J Biochem. 2003;270:1838-49 pubmed
    ..The enzyme is inhibited by EDTA and shows only weak activity when replacing Mg2+ with Ca2+ ions. ..

More Information


  1. Lucht J, Boos W, Bremer E. Alignment of genes from the 9-minute region (araJ to tsx) of the Escherichia coli K-12 linkage map to the physical map. J Bacteriol. 1992;174:1709-10 pubmed
  2. Cavalcanti M, Steilmann C, Failing K, Bergmann M, Kliesch S, Weidner W, et al. Apoptotic gene expression in potentially fertile and subfertile men. Mol Hum Reprod. 2011;17:415-20 pubmed publisher
    ..Our data suggest that Casp4, Casp 6 and TNFSF10 are differentially expressed in potentially fertile and subfertile men and represent useful biomarkers for predicting male fertility in combination with P1 and P2. ..
  3. Heun M, Binnenkade L, Kreienbaum M, Thormann K. Functional specificity of extracellular nucleases of Shewanella oneidensis MR-1. Appl Environ Microbiol. 2012;78:4400-11 pubmed publisher
    ..The findings strongly suggest that the extracellular nucleases of S. oneidensis exert specific functions required under different conditions...
  4. Kutscher D, Pingoud A, Jeltsch A, Meiss G. Identification of ICAD-derived peptides capable of inhibiting caspase-activated DNase. FEBS J. 2012;279:2917-28 pubmed publisher
    ..In these assays, two ICAD-derived peptides were identified that are capable of efficiently and specifically inhibiting CAD activity in solution. ..
  5. Matallana Ramirez L, Rauf M, Farage Barhom S, Dortay H, Xue G, Dröge Laser W, et al. NAC transcription factor ORE1 and senescence-induced BIFUNCTIONAL NUCLEASE1 (BFN1) constitute a regulatory cascade in Arabidopsis. Mol Plant. 2013;6:1438-52 pubmed publisher
    ..We also demonstrate binding of ORE1 in vivo to the promoters of two other senescence-associated genes, namely SAG29/SWEET15 and SINA1, supporting the central role of ORE1 during senescence. ..
  6. Plagens A, Tripp V, Daume M, Sharma K, Klingl A, Hrle A, et al. In vitro assembly and activity of an archaeal CRISPR-Cas type I-A Cascade interference complex. Nucleic Acids Res. 2014;42:5125-38 pubmed publisher
    ..The reconstituted Cascade was used to identify sequence motifs that are required for efficient DNA degradation and to investigate the role of the subunits Cas7 and Cas3'' in the interplay with other Cascade subunits. ..
  7. Herber B, Truss M, Beato M, Muller R. Inducible regulatory elements in the human cyclin D1 promoter. Oncogene. 1994;9:1295-304 pubmed
    ..Cotransfection experiments showed that the cyclin D1 promoter is inducible by c-Jun, and that this induction is mediated predominantly through the protected putative CRE at -52. ..
  8. Jekel M, Wackernagel W. The periplasmic endonuclease I of Escherichia coli has amino-acid sequence homology to the extracellular DNases of Vibrio cholerae and Aeromonas hydrophila. Gene. 1995;154:55-9 pubmed
    ..The growth rate and viability of the cells was not affected. The predicted aa sequence of the ORF is 60 and 54% identical to the sequence of extracellular DNases from Vibrio cholerae and Aeromonas hydrophila, respectively. ..
  9. Grossmann K, Kwiatkowski J, Tresch S. Auxin herbicides induce H(2)O(2) overproduction and tissue damage in cleavers (Galium aparine L.). J Exp Bot. 2001;52:1811-6 pubmed
    ..This overproduction of H(2)O(2) could be triggered by the decline of photosynthetic activity, due to ABA-mediated stomatal closure. ..