Genomes and Genes
Experts and Doctors on cultured cells in Germany
Topic: cultured cells
Publications443 found, 100 shown here
- Weis Klemm M, Alexander D, Pap T, Schützle H, Reyer D, Franz J, et al. Synovial fibroblasts from rheumatoid arthritis patients differ in their regulation of IL-16 gene activity in comparison to osteoarthritis fibroblasts. Cell Physiol Biochem. 2004;14:293-300 pubmed..The enhanced expression of IL-16 in the synovial membrane early in RA vs OA may be associated in part with these distinct signaling responses. ..
- Das Gupta R, Krause Ihle T, Bergmann B, Müller I, Khomutov A, Muller S, et al. 3-Aminooxy-1-aminopropane and derivatives have an antiproliferative effect on cultured Plasmodium falciparum by decreasing intracellular polyamine concentrations. Antimicrob Agents Chemother. 2005;49:2857-64 pubmed..In contrast, the ODC inhibitors led to decreased cellular putrescine and spermidine levels in P. falciparum, supporting the fact that they exert their antimalarial activities by inhibition of the bifunctional ODC-AdoMetDC...
- Eisele K, Lang P, Kempe D, Klarl B, Niemöller O, Wieder T, et al. Stimulation of erythrocyte phosphatidylserine exposure by mercury ions. Toxicol Appl Pharmacol. 2006;210:116-22 pubmed..Moreover, Hg2+ increases ceramide formation. The observed mechanisms could similarly participate in the triggering of apoptosis in nucleated cells by Hg2+. ..
- Schulze K, Medina E, Guzman C. Intranasal immunization with serum opacity factor (SOF) of Streptococcus pyogenes fails to protect mice against lethal mucosal challenge with a heterologous strain. Vaccine. 2006;24:1446-50 pubmed..These results highlight the importance of developing adequate experimental animal models to evaluate vaccine efficiency, according to the selected antigen. ..
- van der Ven P, Ehler E, Vakeel P, Eulitz S, Schenk J, Milting H, et al. Unusual splicing events result in distinct Xin isoforms that associate differentially with filamin c and Mena/VASP. Exp Cell Res. 2006;312:2154-67 pubmed
- Gergs U, Berndt T, Buskase J, Jones L, Kirchhefer U, Müller F, et al. On the role of junctin in cardiac Ca2+ handling, contractility, and heart failure. Am J Physiol Heart Circ Physiol. 2007;293:H728-34 pubmed..Our results demonstrate that an increased expression of junctin is associated with an impaired Ca(2+) homeostasis. Downregulation of junctin in human heart failure may thus be a compensatory mechanism. ..
- Vömel M, Wegener C. Neurotransmitter-induced changes in the intracellular calcium concentration suggest a differential central modulation of CCAP neuron subsets in Drosophila. Dev Neurobiol. 2007;67:792-808 pubmed..Our results suggest that N(CCAP) express functional receptors for ACh, glutamate, and GABA, and indicate that these transmitters are involved in a context-dependent regulation of functionally distinct N(CCAP) subsets. ..
- Bridger P, Menge C, Leiser R, Tinneberg H, Pfarrer C. Bovine caruncular epithelial cell line (BCEC-1) isolated from the placenta forms a functional epithelial barrier in a polarised cell culture model. Placenta. 2007;28:1110-7 pubmed
- Iandiev I, Pannicke T, Hollborn M, Wiedemann P, Reichenbach A, Grimm C, et al. Localization of glial aquaporin-4 and Kir4.1 in the light-injured murine retina. Neurosci Lett. 2008;434:317-21 pubmed publisher..1 and aquaporin-4 proteins; this alteration is thought to be a response to the edema in the outer retina and may support the resolution of edema. ..
- Brenneis C, Coste O, Schmidt R, Angioni C, Popp L, Nusing R, et al. Consequences of altered eicosanoid patterns for nociceptive processing in mPGES-1-deficient mice. J Cell Mol Med. 2008;12:639-48 pubmed publisher..Since the latter prostaglandins serve also as mediators of nociception they may compensate the loss of PGE(2) synthesis in mPGES-1-deficient mice. ..
- Lehrnbecher T, Beck O, Koehl U, Tramsen L. Cultivated anti-Aspergillus T(H)1 cells. Med Mycol. 2009;47 Suppl 1:S170-4 pubmed publisher..However, before performing clinical trials, open questions such as which patient population will benefit from adoptive immunotherapy with anti-Aspergillus T cells have to be addressed. ..
- Schwerdt G, Königs M, Holzinger H, Humpf H, Gekle M. Effects of the mycotoxin fumonisin B(1) on cell death in human kidney cells and human lung fibroblasts in primary culture. J Appl Toxicol. 2009;29:174-82 pubmed publisher..Furthermore LC-MS/MS studies did not give any hints for a metabolism of FB(1). Therefore, the main risk of prolonged FB(1) exposure seems to be altered collagen secretion pattern. ..
- Glassmann A, Topka S, Wang Eckardt L, Anders S, Weisheit G, Endl E, et al. Basic molecular fingerprinting of immature cerebellar cortical inhibitory interneurons and their precursors. Neuroscience. 2009;159:69-82 pubmed publisher..Together, these data support the notion that diversification of cerebellar inhibitory interneurons is highly regulative and subject to local signaling to postmigratory precursors. ..
- Zippel J, Deters A, Pappai D, Hensel A. A high molecular arabinogalactan from Ribes nigrum L.: influence on cell physiology of human skin fibroblasts and keratinocytes and internalization into cells via endosomal transport. Carbohydr Res. 2009;344:1001-8 pubmed publisher..The FITC-labeled polysaccharide was incorporated in a time-dependent manner into human fibroblasts (laser scanning microscopy) via endosomal transport. This internalization of the polysaccharide was inhibited by Cytochalasin B. ..
- Hippe H, Wolf N, Abu Taha I, Mehringer R, Just S, Lutz S, et al. The interaction of nucleoside diphosphate kinase B with Gbetagamma dimers controls heterotrimeric G protein function. Proc Natl Acad Sci U S A. 2009;106:16269-74 pubmed publisher..Thus, our results suggest a role for the interaction of NDPK B with Gbetagamma dimers and caveolins in regulating membranous G protein content and maintaining normal G protein function in vivo. ..
- Sebastiano V, Dalvai M, Gentile L, Schubart K, Sutter J, Wu G, et al. Oct1 regulates trophoblast development during early mouse embryogenesis. Development. 2010;137:3551-60 pubmed publisher..Our data also suggest that Oct1 is required in the embryo proper from late gastrulation stages onwards. ..
- Trautmann B, Schlitt H, Hahn E, Lohr M. Isolation, culture, and characterization of human pancreatic duct cells. Pancreas. 1993;8:248-54 pubmed..The cultured normal human duct cells as the postulated cells of origin of the pancreatic adenocarcinoma may serve as a useful control for cultured pancreatic tumor cell lines. ..
- Ashery U, Koch H, Scheuss V, Brose N, Rettig J. A presynaptic role for the ADP ribosylation factor (ARF)-specific GDP/GTP exchange factor msec7-1. Proc Natl Acad Sci U S A. 1999;96:1094-9 pubmed..Our results provide evidence that small G proteins of the ARF family and activating factors like msec7-1 play an important role in synaptic transmission, most likely by making more vesicles available for fusion at the plasma membrane. ..
- Garlichs C, Zhang H, Mugge A, Daniel W. Beta-blockers reduce the release and synthesis of endothelin-1 in human endothelial cells. Eur J Clin Invest. 1999;29:12-6 pubmed..The aim of this study was to determine whether beta-adrenergic antagonists have an influence on endothelin-1 (ET-1) synthesis and release in human endothelial cells...
- Talts J, Sasaki T, Miosge N, Göhring W, Mann K, Mayne R, et al. Structural and functional analysis of the recombinant G domain of the laminin alpha4 chain and its proteolytic processing in tissues. J Biol Chem. 2000;275:35192-9 pubmed..Immunogold staining demonstrated an association of the alpha4 chain primarily with microfibrillar regions rather than with basement membranes, while laminin alpha2 chains appear primarily associated with various basement membranes. ..
- Medana I, Li Z, Flügel A, Tschopp J, Wekerle H, Neumann H. Fas ligand (CD95L) protects neurons against perforin-mediated T lymphocyte cytotoxicity. J Immunol. 2001;167:674-81 pubmed..5 h, reflecting perforin-dependent cell lysis. FasL seems to protect neurons by blocking degranulation of CTLs, since CD3-induced release of cytotoxic granules was reduced by coapplication of Fas-specific Abs or rFasL. ..
- zur Nieden N, Kempka G, Ahr H. In vitro differentiation of embryonic stem cells into mineralized osteoblasts. Differentiation. 2003;71:18-27 pubmed..The in vitro differentiation of mouse embryonic stem cells into osteoblasts may provide a suitable model for studying the molecular processes of osteoblastic development in vivo. ..
- Böhm M, Sawicka K, Siebrasse J, Brehmer Fastnacht A, Peters R, Klempnauer K. The transformation suppressor protein Pdcd4 shuttles between nucleus and cytoplasm and binds RNA. Oncogene. 2003;22:4905-10 pubmed..Taken together, our data raise the possibility that Pdcd4 is involved in some aspect of nuclear RNA metabolism in addition to its suspected role in protein translation. ..
- Wiendl H, Mitsdoerffer M, Schneider D, Chen L, Lochmuller H, Melms A, et al. Human muscle cells express a B7-related molecule, B7-H1, with strong negative immune regulatory potential: a novel mechanism of counterbalancing the immune attack in idiopathic inflammatory myopathies. FASEB J. 2003;17:1892-4 pubmed..These data highlight the immune regulatory properties of muscle cells and suggest that B7-H1 expression represents an inhibitory mechanism induced upon inflammatory stimuli and aimed at protecting muscle fibers from immune aggression. ..
- Kubitz R, Saha N, Kühlkamp T, Dutta S, vom Dahl S, Wettstein M, et al. Ca2+-dependent protein kinase C isoforms induce cholestasis in rat liver. J Biol Chem. 2004;279:10323-30 pubmed..The data identify Ca(2+)-dependent PKC isoforms as inducers of cholestasis. This is mainly due to inhibition of taurocholate excretion involving transporter retrieval from the canalicular membrane. ..
- Glatt H, Pabel U, Meinl W, Frederiksen H, Frandsen H, Muckel E. Bioactivation of the heterocyclic aromatic amine 2-amino-3-methyl-9H-pyrido [2,3-b]indole (MeAalphaC) in recombinant test systems expressing human xenobiotic-metabolizing enzymes. Carcinogenesis. 2004;25:801-7 pubmed..Another minor activation pathway involved benzylic hydroxylation followed by sulpho conjugation by SULT1A1. ..
- Beck S, Beck G, Ostendorf T, Floege J, Lambeau G, Nevalainen T, et al. Upregulation of group IB secreted phospholipase A(2) and its M-type receptor in rat ANTI-THY-1 glomerulonephritis. Kidney Int. 2006;70:1251-60 pubmed..Subsequently, both sPLA(2)-IB and the M-type sPLA(2) receptor are involved in the autocrine and paracrine amplification of the inflammatory process in different resident and infiltrating cells. ..
- Zhou L, Lehan N, Wehrenberg U, Disteldorf E, von Lossow R, Mares U, et al. Neuroprotection by estradiol: a role of aromatase against spine synapse loss after blockade of GABA(A) receptors. Exp Neurol. 2007;203:72-81 pubmed..Re-filling of estradiol stores after their depletion due to overexcitation may therefore add to therapeutical strategies in epilepsy. ..
- Fritzsching B, Korporal M, Haas J, Krammer P, Suri Payer E, Wildemann B. Similar sensitivity of regulatory T cells towards CD95L-mediated apoptosis in patients with multiple sclerosis and healthy individuals. J Neurol Sci. 2006;251:91-7 pubmed..Furthermore, as cell death is not enhanced, therapeutic expansion of MS-T(reg)in vitro should be a reasonable and novel therapeutic option. ..
- Heinen S, Jozsi M, Hartmann A, Noris M, Remuzzi G, Skerka C, et al. Hemolytic uremic syndrome: a factor H mutation (E1172Stop) causes defective complement control at the surface of endothelial cells. J Am Soc Nephrol. 2007;18:506-14 pubmed..These effects explain how mutant factor H causes defective complement control and in HUS-particularly under condition of inflammation and complement activation-causes endothelial cell damage...
- Henke N, Schmidt Ullrich R, Dechend R, Park J, Qadri F, Wellner M, et al. Vascular endothelial cell-specific NF-kappaB suppression attenuates hypertension-induced renal damage. Circ Res. 2007;101:268-76 pubmed..We conclude that in vivo NF-kappaB suppression in endothelial cells stops a signaling cascade leading to reduced hypertension-induced renal damage despite high blood pressure...
- Roosterman D, Cottrell G, Padilla B, Muller L, Eckman C, Bunnett N, et al. Endothelin-converting enzyme 1 degrades neuropeptides in endosomes to control receptor recycling. Proc Natl Acad Sci U S A. 2007;104:11838-43 pubmed..Antagonism of these endosomal processes represents a strategy for inhibiting sustained signaling of receptors, and defects may explain the tachyphylaxis of drugs that are receptor agonists. ..
- Joussen N, Heckel D, Haas M, Schuphan I, Schmidt B. Metabolism of imidacloprid and DDT by P450 CYP6G1 expressed in cell cultures of Nicotiana tabacum suggests detoxification of these insecticides in Cyp6g1-overexpressing strains of Drosophila melanogaster, leading to resistance. Pest Manag Sci. 2008;64:65-73 pubmed..Thus, the gap between the supposed resistance gene Cyp6g1 and the observed resistance phenomenon was closed by the evidence that CYP6G1 is capable of metabolising at least two insecticides. ..
- Kobsar A, Heeg S, Krohne K, Opitz A, Walter U, Bock M, et al. Cyclic nucleotide-regulated proliferation and differentiation vary in human hematopoietic progenitor cells derived from healthy persons, tumor patients, and chronic myelocytic leukemia patients. Stem Cells Dev. 2008;17:81-91 pubmed publisher..Pharmacological strategies using cyclic nucleotide-elevating substances to influence HPC growth and differentiation in the bone marrow might support current strategies in HPC recovery from the peripheral blood. ..
- Schroeter M, Leifheit M, Sudholt P, Heida N, Dellas C, Rohm I, et al. Leptin enhances the recruitment of endothelial progenitor cells into neointimal lesions after vascular injury by promoting integrin-mediated adhesion. Circ Res. 2008;103:536-44 pubmed publisher..Our findings suggest that leptin specifically modulates the adhesive properties and the homing potential of EPCs and may thus enhance their capacity to promote vascular regeneration in vivo. ..
- Gassanov N, Er F, Michels G, Zagidullin N, Brandt M, Hoppe U. Divergent regulation of cardiac KCND3 potassium channel expression by the thyroid hormone receptors alpha1 and beta1. J Physiol. 2009;587:1319-29 pubmed publisher..Thus, I(to) is modulated by diverse T3-dependent regulation of underlying gene transcription. TRalpha1 and TRbeta1 exhibit distinct effects on KCND3 transactivation with TRalpha1 enhancing and TRbeta1 suppressing KCND3 transcription. ..
- Kieper N, HolmstrÃ¶m K, Ciceri D, Fiesel F, Wolburg H, Ziviani E, et al. Modulation of mitochondrial function and morphology by interaction of Omi/HtrA2 with the mitochondrial fusion factor OPA1. Exp Cell Res. 2010;316:1213-24 pubmed publisher..Our results underscore a critical role of impaired mitochondrial dynamics in neurodegenerative disorders. ..
- Theisen C, Fuchs Winkelmann S, Knappstein K, Efe T, Schmitt J, Paletta J, et al. Influence of nanofibers on growth and gene expression of human tendon derived fibroblast. Biomed Eng Online. 2010;9:9 pubmed publisher..However, irrespective of the chemical composition of the fibres, the collagen deposition was altered, an effect which might be associated with a decreased expression of biglycanes. ..
- Rossmann H, Sonnentag T, Heinzmann A, Seidler B, Bachmann O, Vieillard Baron D, et al. Differential expression and regulation of Na(+)/H(+) exchanger isoforms in rabbit parietal and mucous cells. Am J Physiol Gastrointest Liver Physiol. 2001;281:G447-58 pubmed..The high NHE1 and NHE2 expression levels in mucous cells may reflect their special need for pH(i) regulation during high gastric acidity. NHE4 is likely involved in volume regulation during acid secretion...
- Ignatov A, Lintzel J, Kreienkamp H, Schaller H. Sphingosine-1-phosphate is a high-affinity ligand for the G protein-coupled receptor GPR6 from mouse and induces intracellular Ca2+ release by activating the sphingosine-kinase pathway. Biochem Biophys Res Commun. 2003;311:329-36 pubmed..The antiapoptotic effect of S1P required activation of sphingosine kinase and was accompanied by an increase in MAP-kinase phosphorylation. ..
- Schramme A, Abdel Bakky M, Kämpfer Kolb N, Pfeilschifter J, Gutwein P. The role of CXCL16 and its processing metalloproteinases ADAM10 and ADAM17 in the proliferation and migration of human mesangial cells. Biochem Biophys Res Commun. 2008;370:311-6 pubmed publisher..This finding could be important to develop novel diagnostic and therapeutic strategies to treat mesangial proliferative kidney diseases. ..
- Maier M, Geiger E, Henrich D, Bendt C, Wutzler S, Lehnert M, et al. Platelet factor 4 is highly upregulated in dendritic cells after severe trauma. Mol Med. 2009;15:384-91 pubmed publisher..PF4 also participates in the posttraumatic activation of DCs in relation to injury severity, a role that might be preferably based on the modification of receptor expression, whereas adhesion properties are largely unaffected. ..
- Broer S, Rahman B, Pellegri G, Pellerin L, Martin J, Verleysdonk S, et al. Comparison of lactate transport in astroglial cells and monocarboxylate transporter 1 (MCT 1) expressing Xenopus laevis oocytes. Expression of two different monocarboxylate transporters in astroglial cells and neurons. J Biol Chem. 1997;272:30096-102 pubmed..These data provide further molecular support for the existence of a lactate shuttle between astrocytes and neurons. ..
- Hogger P, Sorg C. Soluble CD163 inhibits phorbol ester-induced lymphocyte proliferation. Biochem Biophys Res Commun. 2001;288:841-3 pubmed..The highly purified protein statistically significantly inhibits phorbol ester-induced human T-lymphocyte activation, thus attenuating the immune response to the inflammatory mediator. ..
- Zanner R, Hapfelmeier G, Gratzl M, Prinz C. Intracellular signal transduction during gastrin-induced histamine secretion in rat gastric ECL cells. Am J Physiol Cell Physiol. 2002;282:C374-82 pubmed..Gastrin-induced calcium influx was mediated by dihydropyridine-sensitive calcium channels that appear to be L-type channels activated through a pathway involving activation of PKC. ..
- Eberhardt W, Schulze M, Engels C, Klasmeier E, Pfeilschifter J. Glucocorticoid-mediated suppression of cytokine-induced matrix metalloproteinase-9 expression in rat mesangial cells: involvement of nuclear factor-kappaB and Ets transcription factors. Mol Endocrinol. 2002;16:1752-66 pubmed..In addition to NF-kappaB, we suggest a pivotal role for the Ets binding site, in concert with a distal activator protein-1 element, in the transcriptional suppression of cytokine-induced MMP-9 expression by glucocorticoids. ..
- Kessels M, Qualmann B. Syndapins integrate N-WASP in receptor-mediated endocytosis. EMBO J. 2002;21:6083-94 pubmed
- Muller F, Müller Röver S, Korge B, Kapas S, Hinson J, Philpott M. Adrenomedullin: expression and possible role in human skin and hair growth. Br J Dermatol. 2003;148:30-8 pubmed..This finding is consistent with the proposed autocrine/paracrine mechanism in the physiology of AM. ..
- Ehrenreich H, Degner D, Meller J, Brines M, Behe M, Hasselblatt M, et al. Erythropoietin: a candidate compound for neuroprotection in schizophrenia. Mol Psychiatry. 2004;9:42-54 pubmed
- Fischer Posovszky P, Tornqvist H, Debatin K, Wabitsch M. Inhibition of death-receptor mediated apoptosis in human adipocytes by the insulin-like growth factor I (IGF-I)/IGF-I receptor autocrine circuit. Endocrinology. 2004;145:1849-59 pubmed..In conclusion, we identified mechanisms of apoptosis induction in human fat cells. We furthermore demonstrate that human fat cells protect themselves from apoptosis by IGF-I in an auto-/paracrine manner. ..
- Bräuer A, Nitsch R, Savaskan N. Identification of macrophage/microglia activation factor (MAF) associated with late endosomes/lysosomes in microglial cells. FEBS Lett. 2004;563:41-8 pubmed..In conclusion, these results imply that MAF is involved in the dynamics of lysosomal membranes associated with microglial activation following brain lesion. ..
- Stawowy P, Margeta C, Kallisch H, Seidah N, Chretien M, Fleck E, et al. Regulation of matrix metalloproteinase MT1-MMP/MMP-2 in cardiac fibroblasts by TGF-beta1 involves furin-convertase. Cardiovasc Res. 2004;63:87-97 pubmed..Thus, furin is central for TGF-beta1 and MT1-MMP activation and might be a novel target in cardiac remodeling. ..
- Thiele B, Doller A, Kahne T, Pregla R, Hetzer R, Regitz Zagrosek V. RNA-binding proteins heterogeneous nuclear ribonucleoprotein A1, E1, and K are involved in post-transcriptional control of collagen I and III synthesis. Circ Res. 2004;95:1058-66 pubmed..5- and 4.5-fold relative to controls. ..
- Stawowy P, Meyborg H, Stibenz D, Borges Pereira Stawowy N, Roser M, Thanabalasingam U, et al. Furin-like proprotein convertases are central regulators of the membrane type matrix metalloproteinase-pro-matrix metalloproteinase-2 proteolytic cascade in atherosclerosis. Circulation. 2005;111:2820-7 pubmed..Furin and PC5 are expressed in human peripheral blood mononuclear cells and colocalize with MT1-MMP in macrophages in the atherosclerotic plaque, supporting the hypothesis that they are potential targets in atherosclerosis. ..
- Wornle M, Schmid H, Banas B, Merkle M, Henger A, Roeder M, et al. Novel role of toll-like receptor 3 in hepatitis C-associated glomerulonephritis. Am J Pathol. 2006;168:370-85 pubmed..Thus, TLR3 expression on renal cells, and especially MCs, may establish a link between viral infections and glomerular diseases. ..
- Christiansen H, Batusic D, Saile B, Hermann R, Dudas J, Rave Frank M, et al. Identification of genes responsive to gamma radiation in rat hepatocytes and rat liver by cDNA array gene expression analysis. Radiat Res. 2006;165:318-25 pubmed..Thus we identified genes that may be involved in liver steatosis after irradiation. The methods shown in this work should help to further clarify the consequences of radiation exposure in the liver. ..
- Meerbach A, Meier C, Sauerbrei A, Meckel H, Wutzler P. Antiviral activity of cyclosaligenyl prodrugs of the nucleoside analogue bromovinyldeoxyuridine against herpes viruses. Int J Antimicrob Agents. 2006;27:423-30 pubmed..Most remarkably, several cycloSal-BVDUMP triesters yielded promising anti-EBV activity whereas the parent compound BVDU was entirely inactive. ..
- Wissel H, Burkhardt W, Rupp J, Wauer R, Rudiger M. Perfluorocarbons decrease Chlamydophila pneumoniae-mediated inflammatory responses of rat type II pneumocytes in vitro. Pediatr Res. 2006;60:264-9 pubmed..Since PF5080 was found in ATII-cell membranes, PF5080 effect could be explained by an alteration of the cellular membrane, preventing activation of the inflammatory cascade. ..
- Gensch C, Clever Y, Werner C, Hanhoun M, Bohm M, Laufs U. The PPAR-gamma agonist pioglitazone increases neoangiogenesis and prevents apoptosis of endothelial progenitor cells. Atherosclerosis. 2007;192:67-74 pubmed..Pioglitazone prevents apoptosis of EPC in mice as well as in human EPC in a PI3K-dependent but NO-independent manner. Reduction of EPC apoptosis by TZD may be a potentially beneficial mechanism for patients with vascular diseases. ..
- Groemer T, Klingauf J. Synaptic vesicles recycling spontaneously and during activity belong to the same vesicle pool. Nat Neurosci. 2007;10:145-7 pubmed..Our results provide evidence as to the identities of the vesicle pools recycling at rest and during stimulation. ..
- Schestkowa O, Geisel D, Jacob R, Hasilik A. The catalytically inactive precursor of cathepsin D induces apoptosis in human fibroblasts and HeLa cells. J Cell Biochem. 2007;101:1558-66 pubmed
- Sigruener A, Buechler C, Bared S, Grandl M, Aslanidis C, Ugocsai P, et al. E-LDL upregulates TOSO expression and enhances the survival of human macrophages. Biochem Biophys Res Commun. 2007;359:723-8 pubmed..In COS-7 cells however, phagocytotic activity was not affected by TOSO. These data indicate that E-LDL-generated foam cells are protected from cell death most likely through the expression of TOSO by a FLIP(L) independent mechanism. ..
- Kralj A, Gurgui M, König G, van Echten Deckert G. Trichothecenes induce accumulation of glucosylceramide in neural cells by interfering with lactosylceramide synthase activity. Toxicol Appl Pharmacol. 2007;225:113-22 pubmed..Collectively, our results demonstrate that trichodermol and 8-deoxy-trichothecin inhibit LacCer synthase activity in a cell-type-specific manner. ..
- Choi C, Reimers K, Allmeling C, Kall S, Choi Y, Vogt P. Inhibition of apoptosis by expression of antiapoptotic proteins in recombinant human keratinocytes. Cell Transplant. 2007;16:663-74 pubmed..66), Faim (p = 0.42), or LFG (p = 0.44) expressing cells. Our results suggest that heterotopic expression of antiapoptotic proteins can induce the resistance of keratinocytes to a major mechanism of rejection. ..
- Mohrlüder J, Stangler T, Hoffmann Y, Wiesehan K, Mataruga A, Willbold D. Identification of calreticulin as a ligand of GABARAP by phage display screening of a peptide library. FEBS J. 2007;274:5543-55 pubmed..Calreticulin and GABARAP interact with a dissociation constant K(d) = 64 nm and a mean lifetime of the complex of 20 min. Thus, the interaction between GABARAP and calreticulin is the strongest so far reported for each protein. ..
- Candelario Jalil E, de Oliveira A, Gräf S, Bhatia H, Hull M, Munoz E, et al. Resveratrol potently reduces prostaglandin E2 production and free radical formation in lipopolysaccharide-activated primary rat microglia. J Neuroinflammation. 2007;4:25 pubmed..These findings suggest that the naturally occurring polyphenol resveratrol is able to reduce microglial activation, an effect that might help to explain its neuroprotective effects in several in vivo models of brain injury. ..
- Bayindir U, Alfermann A, Fuss E. Hinokinin biosynthesis in Linum corymbulosum Reichenb. Plant J. 2008;55:810-20 pubmed publisher..Our results strongly indicate that PLR-Lc1 participates in (-)-hinokinin biosynthesis in L. corymbulosum by the first of the two hypothetical pathways via (-)-secoisolariciresinol. ..
- Zeuschner D, Mildner K, Zaehres H, Scholer H. Induced pluripotent stem cells at nanoscale. Stem Cells Dev. 2010;19:615-20 pubmed publisher..Both cell types are almost indistinguishable at the ultrastructural level, providing further evidence for the similarity of these 2 pluripotent stem cell populations. ..
- König T, Bogdan C, Schleicher U. Translational repression of inducible NO synthase in macrophages by l-arginine depletion is not associated with an increased phosphorylation of eIF2alpha. Immunobiology. 2009;214:822-7 pubmed publisher..Alternative modes of translational repression of iNOS need to be considered. ..
- Zitta K, Brandt B, Wuensch A, Meybohm P, Bein B, Steinfath M, et al. Interleukin-1beta regulates cell proliferation and activity of extracellular matrix remodelling enzymes in cultured primary pig heart cells. Biochem Biophys Res Commun. 2010;399:542-7 pubmed publisher..Combined with our recently published in vivo data (Meybohm et al., PLoS One, 2009), the results presented here strongly suggest IL-1beta as a key molecule guiding tissue remodelling events after myocardial infarction...
- Tsarovina K, Reiff T, Stubbusch J, Kurek D, Grosveld F, Parlato R, et al. The Gata3 transcription factor is required for the survival of embryonic and adult sympathetic neurons. J Neurosci. 2010;30:10833-43 pubmed publisher..These results demonstrate that Gata3 is continuously required for maintaining survival but not differentiation in the sympathetic neuron lineage up to mature neurons of adult animals. ..
- Rathke Hartlieb S, Budde P, Ewert S, Schlomann U, Staege M, Jockusch H, et al. Elevated expression of membrane type 1 metalloproteinase (MT1-MMP) in reactive astrocytes following neurodegeneration in mouse central nervous system. FEBS Lett. 2000;481:227-34 pubmed..In primary astrocyte cultures, MT1-MMP mRNA was upregulated by exogeneous tumor necrosis factor alpha. Increased plasma membrane and secreted MMP activities were found in primary WR astrocytes. ..
- Derst C, Karschin C, Wischmeyer E, Hirsch J, Preisig Müller R, Rajan S, et al. Genetic and functional linkage of Kir5.1 and Kir2.1 channel subunits. FEBS Lett. 2001;491:305-11 pubmed..When expressed in Xenopus oocytes, Kir5.1 is efficiently targeted to the cell surface and forms electrically silent channels together with Kir2.1, thus negatively controlling Kir2.1 channel activity in native cells. ..
- Gaumann A, Laudes M, Jacob B, Pommersheim R, Laue C, Vogt W, et al. Xenotransplantation of parathyroids in rats using barium-alginate and polyacrylic acid multilayer microcapsules. Exp Toxicol Pathol. 2001;53:35-43 pubmed..In contrast minced tissue underwent necrosis and induced significantly more connective tissue reaction than single cells indicating an interrelationship between necrosis and fibrosis. ..
- Schling P, Loffler G. Effects of angiotensin II on adipose conversion and expression of genes of the renin-angiotensin system in human preadipocytes. Horm Metab Res. 2001;33:189-95 pubmed..Further studies are needed to find out whether the effects of angiotensin II on the renin-angiotensin system are direct feedback loops or secondary to changes in the differentiation program. ..
- Peitz M, Pfannkuche K, Rajewsky K, Edenhofer F. Ability of the hydrophobic FGF and basic TAT peptides to promote cellular uptake of recombinant Cre recombinase: a tool for efficient genetic engineering of mammalian genomes. Proc Natl Acad Sci U S A. 2002;99:4489-94 pubmed..We expect that application of His-TAT-NLS-Cre, which can be produced readily in large quantities from a bacterial source, will expand our abilities to manipulate mammalian genomes. ..
- Gil Parrado S, Fernandez Montalvan A, Assfalg Machleidt I, Popp O, Bestvater F, Holloschi A, et al. Ionomycin-activated calpain triggers apoptosis. A probable role for Bcl-2 family members. J Biol Chem. 2002;277:27217-26 pubmed..We conclude that ionomycin-induced calpain activation promotes decrease of Bcl-2 proteins thereby triggering the intrinsic apoptotic pathway. ..
- Schöniger S, Maronde E, Kopp M, Korf H, Nurnberger F. Transcription factor CREB and its stimulus-dependent phosphorylation in cell and explant cultures of the bovine subcommissural organ. Cell Tissue Res. 2002;308:131-42 pubmed..SP and ATP stimulate the phosphorylation of CREB apparently via a calcium-dependent mechanism and are thus involved in the control of gene expression in the bovine SCO. ..
- Peterziel H, Unsicker K, Krieglstein K. TGFbeta induces GDNF responsiveness in neurons by recruitment of GFRalpha1 to the plasma membrane. J Cell Biol. 2002;159:157-67 pubmed..Our data suggest that TGFbeta is involved in GFRalpha1 membrane translocation, thereby permitting GDNF signaling and neurotrophic effects. ..
- Cacace S, Schröder G, Wehinger E, Strack D, Schmidt J, Schröder J. A flavonol O-methyltransferase from Catharanthus roseus performing two sequential methylations. Phytochemistry. 2003;62:127-37 pubmed..The resulting methylation pattern is characteristic for C. roseus flavonol glycosides and anthocyanins, and it is proposed that CrOMT2 is involved in their biosynthesis. ..
- Staiger H, Kaltenbach S, Staiger K, Stefan N, Fritsche A, Guirguis A, et al. Expression of adiponectin receptor mRNA in human skeletal muscle cells is related to in vivo parameters of glucose and lipid metabolism. Diabetes. 2004;53:2195-201 pubmed..AdipoR1, but not AdipoR2, expression correlated with insulin secretion. The molecular nature of this link between muscle and beta-cells needs to be further clarified. ..
- Koleva M, Kappler R, Vogler M, Herwig A, Fulda S, Hahn H. Pleiotropic effects of sonic hedgehog on muscle satellite cells. Cell Mol Life Sci. 2005;62:1863-70 pubmed..Taken together, Shh acts as a proliferation and survival factor of satellite cells in the adult muscle. Our results support the hypothesis of the rhabdomyosarcoma origin from satellite cells and suggest a role for Shh in this process. ..
- Schütz W, Benavente R, Alsheimer M. Dynamic properties of germ line-specific lamin B3: the role of the shortened rod domain. Eur J Cell Biol. 2005;84:649-62 pubmed..Our results lead to the conclusion that lamin B3 reduces the stability of the nuclear periphery. They are also consistent with the notion that lamin B3 is relevant to specific properties of the nuclear envelope during spermiogenesis. ..
- Scheuenpflug J, Krebsfänger N, Doehmer J. Heterologous co-expression of human cytochrome P450 1A2 and polymorphic forms of N-acetyltransferase 2 for studies on aromatic amines in V79 Chinese hamster cells. Altern Lab Anim. 2005;33:561-77 pubmed
- Liu Y, Hao W, Letiembre M, Walter S, Kulanga M, Neumann H, et al. Suppression of microglial inflammatory activity by myelin phagocytosis: role of p47-PHOX-mediated generation of reactive oxygen species. J Neurosci. 2006;26:12904-13 pubmed..These results suggest that intervention in ROS generation could represent a novel therapeutic strategy to reduce neuroinflammation in MS. ..
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- Hamm S, Heit A, Koffler M, Huster K, Akira S, Busch D, et al. Immunostimulatory RNA is a potent inducer of antigen-specific cytotoxic and humoral immune response in vivo. Int Immunol. 2007;19:297-304 pubmed..Additionally, we demonstrate that RNA induces an antigen-specific immunity characterized by a potent cytotoxic T cell response to infection. ..
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- Wiese S, Jablonka S, Holtmann B, Orel N, Rajagopal R, Chao M, et al. Adenosine receptor A2A-R contributes to motoneuron survival by transactivating the tyrosine kinase receptor TrkB. Proc Natl Acad Sci U S A. 2007;104:17210-5 pubmed
- Meuth S, Simon O, Grimm A, Melzer N, Herrmann A, Spitzer P, et al. CNS inflammation and neuronal degeneration is aggravated by impaired CD200-CD200R-mediated macrophage silencing. J Neuroimmunol. 2008;194:62-9 pubmed publisher..CD200 and its receptor could also be detected on neurons and macrophages in human MS plaques. Therefore the CD200-CD200R pathway seems of critical relevance for macrophage-mediated damage in autoimmune inflammation of the CNS. ..