Experts and Doctors on circular dichroism in United Kingdom


Locale: United Kingdom
Topic: circular dichroism

Top Publications

  1. George R, Kelly S, Price N, Erbse A, Fisher M, Lund P. Three GroEL homologues from Rhizobium leguminosarum have distinct in vitro properties. Biochem Biophys Res Commun. 2004;324:822-8 pubmed
    ..This study, the first to compare the properties of three different GroEL homologues from the same organism, shows that despite the high degree of similarity between different homologues, they can display distinct properties in vitro. ..
  2. Li W, Serpell L, Carter W, Rubinsztein D, Huntington J. Expression and characterization of full-length human huntingtin, an elongated HEAT repeat protein. J Biol Chem. 2006;281:15916-22 pubmed
    ..Here we report the expression and preliminary characterization of recombinant full-length wild-type human htt. Our results support a model of htt composed entirely of HEAT repeats that stack to form an elongated superhelix. ..
  3. Wohl A, Troilo H, Collins R, Baldock C, Sengle G. Extracellular Regulation of Bone Morphogenetic Protein Activity by the Microfibril Component Fibrillin-1. J Biol Chem. 2016;291:12732-46 pubmed publisher
    ..Together, these data show that upon prodomain binding to fibrillin-1, the BMP-7 complex undergoes a conformational change, which denies access of BMP receptors to the growth factor. ..
  4. Curnow P, Booth P. Combined kinetic and thermodynamic analysis of alpha-helical membrane protein unfolding. Proc Natl Acad Sci U S A. 2007;104:18970-5 pubmed
    ..1, indicating that the transition state is close to the unfolded state. We thus present a basis for mapping the structural and energetic properties of membrane protein folding by mutagenesis and classical kinetics. ..
  5. Horrocks M, Lee S, Gandhi S, Magdalinou N, Chen S, Devine M, et al. Single-Molecule Imaging of Individual Amyloid Protein Aggregates in Human Biofluids. ACS Chem Neurosci. 2016;7:399-406 pubmed publisher
  6. Vos S, Berrisford D, Avis J. Effect of magnesium ions on the tertiary structure of the hepatitis C virus IRES and its affinity for the cyclic peptide antibiotic viomycin. Biochemistry. 2002;41:5383-96 pubmed
    ..In pseudoknot mutants, Mg(2+) ions have different effects on viomycin binding and thermal stability, suggesting altered tertiary interactions involving subdomain IIId. ..
  7. Rakowska P, Jiang H, Ray S, Pyne A, Lamarre B, Carr M, et al. Nanoscale imaging reveals laterally expanding antimicrobial pores in lipid bilayers. Proc Natl Acad Sci U S A. 2013;110:8918-23 pubmed publisher
    ..The results offer a mechanistic basis for membrane poration as a generic physicochemical process of cooperative and continuous peptide recruitment in the available phospholipid matrix. ..
  8. Bishop K, Mortuza G, Howell S, Yap M, Stoye J, Taylor I. Characterization of an amino-terminal dimerization domain from retroviral restriction factor Fv1. J Virol. 2006;80:8225-35 pubmed
  9. Opaleye O, Rose R, Whittaker M, Woo E, Whittaker J, Pickersgill R. Structural and spectroscopic studies shed light on the mechanism of oxalate oxidase. J Biol Chem. 2006;281:6428-33 pubmed
    ..Combined spectroscopic, biochemical, and structural analyses of mutants confirms the importance of the asparagine residues in organizing a functional active site complex. ..

More Information


  1. Ganesan A, Moore B, Kelly S, Price N, Rolinski O, Birch D, et al. Optical spectroscopic methods for probing the conformational stability of immobilised enzymes. Chemphyschem. 2009;10:1492-9 pubmed publisher
    ..In conclusion, the spectroscopic methods described here provide structural information hitherto inaccessible, with their applicability limited by interference from, rather than the particulate nature of, the support material. ..
  2. Doig A, Baldwin R. N- and C-capping preferences for all 20 amino acids in alpha-helical peptides. Protein Sci. 1995;4:1325-36 pubmed
    ..The data presented here are of value in rationalizing mutations at capping sites in proteins and in predicting the helix contents of peptides. ..
  3. Hirst A, Smith D. Solvent effects on supramolecular gel-phase materials: two-component dendritic gel. Langmuir. 2004;20:10851-7 pubmed
  4. Savic M, Ilic Tomic T, Macmaster R, Vasiljevic B, Conn G. Critical residues for cofactor binding and catalytic activity in the aminoglycoside resistance methyltransferase Sgm. J Bacteriol. 2008;190:5855-61 pubmed publisher
    ..These results represent the first functional characterization of any FmrO methyltransferase and may provide a basis for a further structure-function analysis of these aminoglycoside resistance determinants...
  5. Jean L, Hackett F, Martin S, Blackman M. Functional characterization of the propeptide of Plasmodium falciparum subtilisin-like protease-1. J Biol Chem. 2003;278:28572-9 pubmed
    ..These studies provide new insights into the function of the propeptide in PfSUB-1 activation and shed light on the structural requirements for interaction with the catalytic domain. ..
  6. Oates J, Hicks M, Dafforn T, DiMaio D, Dixon A. In vitro dimerization of the bovine papillomavirus E5 protein transmembrane domain. Biochemistry. 2008;47:8985-92 pubmed publisher
    ..0 kcal mol (-1). We have used our new results to interpret existing models of E5 dimer formation and provide a direct link between TM helix interactions and E5 function...
  7. Smith M, Bateman O, Jaenicke R, Slingsby C. Mutation of interfaces in domain-swapped human betaB2-crystallin. Protein Sci. 2007;16:615-25 pubmed
    ..These are desirable attributes for proteins that have to exist at very high concentration for a very long time. ..
  8. Spencer K, Hiscox J. Expression and structural analysis of infectious bronchitis virus nucleoprotein. Adv Exp Med Biol. 2006;581:133-8 pubmed
  9. Kolstoe S, Ridha B, Bellotti V, Wang N, Robinson C, Crutch S, et al. Molecular dissection of Alzheimer's disease neuropathology by depletion of serum amyloid P component. Proc Natl Acad Sci U S A. 2009;106:7619-23 pubmed publisher
    ..These results support further clinical study of SAP depletion in Alzheimer's disease and potentially other neurodegenerative diseases. ..
  10. Guo J, Luk L, Loveridge E, Allemann R. Thermal adaptation of dihydrofolate reductase from the moderate thermophile Geobacillus stearothermophilus. Biochemistry. 2014;53:2855-63 pubmed publisher
    ..The sum of all these incremental factors allows BsDHFR to function efficiently in the natural habitat of G. stearothermophilus, which is characterized by temperatures that can reach 75 °C. ..
  11. Patel B, Gingras A, Bobkov A, Fujimoto L, Zhang M, Liddington R, et al. The activity of the vinculin binding sites in talin is influenced by the stability of the helical bundles that make up the talin rod. J Biol Chem. 2006;281:7458-67 pubmed
    ..We conclude that the stability of the helical bundles that make up the talin rod is an important factor determining the activity of the individual VBSs. ..
  12. Porcelli I, de Leeuw E, Wallis R, van den Brink van der Laan E, de Kruijff B, Wallace B, et al. Characterization and membrane assembly of the TatA component of the Escherichia coli twin-arginine protein transport system. Biochemistry. 2002;41:13690-7 pubmed
    ..Protease accessibility experiments indicate that the extramembranous region of TatA is located at the cytoplasmic face of the cell membrane. ..
  13. Jones R, Wang M, Jowitt T, Ridley C, Mellody K, Howard M, et al. Fibulin 5 forms a compact dimer in physiological solutions. J Biol Chem. 2009;284:25938-43 pubmed publisher
    ..It is possible that fibulin 5 functions as a dimer during elastinogenesis or that dimerization may provide a method for limiting interactions with binding partners such as tropoelastin. ..
  14. Olsen J, Robert B, Siebert C, Bullough P, Hunter C. Role of the C-terminal extrinsic region of the alpha polypeptide of the light-harvesting 2 complex of Rhodobacter sphaeroides: a domain swap study. Biochemistry. 2003;42:15114-23 pubmed
    ..Hence these new spectral properties have not been accompanied by an alteration in ring size. ..
  15. Marrington R, Small E, Rodger A, Dafforn T, Addinall S. FtsZ fiber bundling is triggered by a conformational change in bound GTP. J Biol Chem. 2004;279:48821-9 pubmed
    ..Therefore, we present "flipping" of the guanine moiety in FtsZ-bound GTP as a mechanism that explains the link between reduced GTPase activity, increased polymer stability, and polymer bundling. ..
  16. McCue H, Patel P, Herbert A, Lian L, Burgoyne R, Haynes L. Solution NMR structure of the Ca2+-bound N-terminal domain of CaBP7: a regulator of golgi trafficking. J Biol Chem. 2012;287:38231-43 pubmed publisher
    ..We propose that these differences in surface hydrophobicity, charge, and methionine content may be important in determining highly specific interactions of CaBP7 with target proteins, such as PI4KIII?. ..
  17. Powl A, O Reilly A, Miles A, Wallace B. Synchrotron radiation circular dichroism spectroscopy-defined structure of the C-terminal domain of NaChBac and its role in channel assembly. Proc Natl Acad Sci U S A. 2010;107:14064-9 pubmed publisher
    ..Analyses of the CTDs of 32 putative prokaryotic Na(V) sequences suggest that a CTD helical bundle is a structural feature conserved throughout the bacterial sodium channel family. ..
  18. Garcia Alai M, Allen M, Joerger A, Bycroft M. The structure of the FYR domain of transforming growth factor beta regulator 1. Protein Sci. 2010;19:1432-8 pubmed publisher
  19. Erat M, Schwarz Linek U, Pickford A, Farndale R, Campbell I, Vakonakis I. Implications for collagen binding from the crystallographic structure of fibronectin 6FnI1-2FnII7FnI. J Biol Chem. 2010;285:33764-70 pubmed publisher
    ..We explore the implications of this model using long collagen peptides and discuss our findings in the context of FN interactions with collagen fibrils. ..
  20. Nicoll A, Trevitt C, Tattum M, Risse E, Quarterman E, Ibarra A, et al. Pharmacological chaperone for the structured domain of human prion protein. Proc Natl Acad Sci U S A. 2010;107:17610-5 pubmed publisher
    ..The identification of a binding site with a defined 3D structure opens up the possibility of designing small molecules that stabilize huPrP and prevent its conversion into the disease-associated form. ..
  21. Tait S, Dutta K, Cowburn D, Warwicker J, Doig A, McCarthy J. Local control of a disorder-order transition in 4E-BP1 underpins regulation of translation via eIF4E. Proc Natl Acad Sci U S A. 2010;107:17627-32 pubmed publisher
    ..This type of order-disorder regulatory mechanism is likely to be relevant to other intermolecular regulatory phenomena in the cell. ..
  22. De Franceschi G, Fecchio C, Sharon R, Schapira A, Proukakis C, Bellotti V, et al. ?-Synuclein structural features inhibit harmful polyunsaturated fatty acid oxidation, suggesting roles in neuroprotection. J Biol Chem. 2017;292:6927-6937 pubmed publisher
    ..Our findings linking aS scavenging activity with brain lipid composition suggest a possible etiological mechanism in some neurodegenerative disorders. ..
  23. Findlay H, Rutherford N, Henderson P, Booth P. Unfolding free energy of a two-domain transmembrane sugar transport protein. Proc Natl Acad Sci U S A. 2010;107:18451-6 pubmed publisher
    ..This study gives insight into the final, critical folding step involving recovery of ligand binding for a multidomain membrane transporter. ..
  24. Le Goff M, Hindson V, Jowitt T, Scott P, Bishop P. Characterization of opticin and evidence of stable dimerization in solution. J Biol Chem. 2003;278:45280-7 pubmed
    ..Dimerization could have a number of functional consequences, including divalent ligand interactions. ..
  25. Belorgey D, Crowther D, Mahadeva R, Lomas D. Mutant Neuroserpin (S49P) that causes familial encephalopathy with neuroserpin inclusion bodies is a poor proteinase inhibitor and readily forms polymers in vitro. J Biol Chem. 2002;277:17367-73 pubmed
    ..These findings provide strong support for the role of neuroserpin polymerization in the formation of the intraneuronal inclusions that are characteristic of FENIB. ..
  26. Fresquet M, Jowitt T, Ylostalo J, Coffey P, Meadows R, Ala Kokko L, et al. Structural and functional characterization of recombinant matrilin-3 A-domain and implications for human genetic bone diseases. J Biol Chem. 2007;282:34634-43 pubmed
    ..Furthermore, we have also determined that the matrilin-3 A-domain appears to bind exclusively to the COL3 domain of type IX collagen and that this binding is abolished in the presence of a disease causing mutation in type IX collagen. ..
  27. Nicholls S, Piper K, Mohammed F, Dafforn T, Tenzer S, Salim M, et al. Secondary anchor polymorphism in the HA-1 minor histocompatibility antigen critically affects MHC stability and TCR recognition. Proc Natl Acad Sci U S A. 2009;106:3889-94 pubmed publisher
    ..Our results define the molecular mechanisms governing immunogenicity of HA-1, and highlight how single amino acid polymorphisms in mHags can critically affect both MHC association and TCR recognition...
  28. Crabtree B, Thiyagarajan N, Prior S, Wilson P, Iyer S, Ferns T, et al. Characterization of human angiogenin variants implicated in amyotrophic lateral sclerosis. Biochemistry. 2007;46:11810-8 pubmed
    ..Our studies on the biochemical and structural features of these ANG variants now form the basis for further investigations to determine their role(s) in ALS. ..
  29. Bailey S, Hayes F. Influence of operator site geometry on transcriptional control by the YefM-YoeB toxin-antitoxin complex. J Bacteriol. 2009;191:762-72 pubmed publisher
    ..Deciphering the molecular basis of toxin-antitoxin transcriptional control will provide key insights into toxin-antitoxin activation and function. ..
  30. Croasdale R, Ivins F, Muskett F, Daviter T, Scott D, Hardy T, et al. An undecided coiled coil: the leucine zipper of Nek2 kinase exhibits atypical conformational exchange dynamics. J Biol Chem. 2011;286:27537-47 pubmed publisher
    ..As a result, the leucine zipper of Nek2 kinase is the first example where the frameshift of coiled-coil heptad repeats has been directly observed experimentally. ..
  31. Duman R, Ishikawa S, Celik I, Strahl H, Ogasawara N, Troć P, et al. Structural and genetic analyses reveal the protein SepF as a new membrane anchor for the Z ring. Proc Natl Acad Sci U S A. 2013;110:E4601-10 pubmed publisher
    ..These helices prefer positively curved membranes due to relaxed lipid density; therefore this type of membrane anchor may assist in keeping the Z ring positioned at the strongly curved leading edge of the developing septum. ..
  32. Adams N, Reid J. The allosteric role of the AAA+ domain of ChlD protein from the magnesium chelatase of synechocystis species PCC 6803. J Biol Chem. 2013;288:28727-32 pubmed publisher
    ..Using a series of mutants in the AAA(+) domain of ChlD, we show that this site is essential for magnesium chelation and allosterically regulates Mg(2+) and MgATP(2-) binding. ..
  33. Liu H, Thaker Y, Stagg L, Schneider H, Ladbury J, Rudd C. SLP-76 sterile α motif (SAM) and individual H5 α helix mediate oligomer formation for microclusters and T-cell activation. J Biol Chem. 2013;288:29539-49 pubmed publisher
    ..Our data identified for the first time a role for the SAM domain in mediating SLP-76 self-association for T-cell function. ..
  34. Patel S, Bui T, Drake A, Fraternali F, Nikolova P. The p73 DNA binding domain displays enhanced stability relative to its homologue, the tumor suppressor p53, and exhibits cooperative DNA binding. Biochemistry. 2008;47:3235-44 pubmed publisher
    ..These stability and DNA binding data for p73 in vitro enhance and complement our understanding of the role of the p73 protein in vivo and could be exploited in designing strategies for cancer therapy in places where p53 is mutated. ..
  35. Clarke T, Kemp G, van Wonderen J, Doyle R, Cole J, Tovell N, et al. Role of a conserved glutamine residue in tuning the catalytic activity of Escherichia coli cytochrome c nitrite reductase. Biochemistry. 2008;47:3789-99 pubmed publisher
  36. Staniland S, Ward B, Harrison A, van der Laan G, Telling N. Rapid magnetosome formation shown by real-time x-ray magnetic circular dichroism. Proc Natl Acad Sci U S A. 2007;104:19524-8 pubmed
  37. Andrews S, Charnock S, Lakey J, Davies G, Claeyssens M, Nerinckx W, et al. Substrate specificity in glycoside hydrolase family 10. Tyrosine 87 and leucine 314 play a pivotal role in discriminating between glucose and xylose binding in the proximal active site of Pseudomonas cellulosa xylanase 10A. J Biol Chem. 2000;275:23027-33 pubmed
    ..These data show that substantial improvements in the ability of Xyl10A to accommodate the C6-OH of glucose-derived substrates are achieved when steric hindrance is removed. ..
  38. White P, Nairn J, Price N, Nimmo H, Coggins J, Hunter I. Phosphoglycerate mutase from Streptomyces coelicolor A3(2): purification and characterization of the enzyme and cloning and sequence analysis of the gene. J Bacteriol. 1992;174:434-40 pubmed
    ..The deduced amino acid sequence is 64% identical to that of the phosphoglycerate mutase of Saccharomyces cerevisiae and has substantial identity to those of other phosphoglycerate mutases. ..
  39. Cronin N, O Reilly A, Duclohier H, Wallace B. Binding of the anticonvulsant drug lamotrigine and the neurotoxin batrachotoxin to voltage-gated sodium channels induces conformational changes associated with block and steady-state activation. J Biol Chem. 2003;278:10675-82 pubmed
  40. Aquilina J, Benesch J, Ding L, Yaron O, Horwitz J, Robinson C. Phosphorylation of alphaB-crystallin alters chaperone function through loss of dimeric substructure. J Biol Chem. 2004;279:28675-80 pubmed
  41. Petersen J, Inoue S, Kelly S, Sullivan S, Kinoshita T, Christie J. Functional characterization of a constitutively active kinase variant of Arabidopsis phototropin 1. J Biol Chem. 2017;292:13843-13852 pubmed publisher
  42. Kennedy M, Garside L, Goodrick L, McDermott L, Brass A, Price N, et al. The Ov20 protein of the parasitic nematode Onchocerca volvulus. A structurally novel class of small helix-rich retinol-binding proteins. J Biol Chem. 1997;272:29442-8 pubmed
    ..The retinol binding activity of Ov20 could possibly contribute to the eye defects associated with onchocerciasis and, because there is no counterpart in mammals, represents a strategic target for chemotherapy. ..
  43. Perrin C, Burkitt W, Perraud X, O Hara J, Jone C. Limited proteolysis and peptide mapping for comparability of biopharmaceuticals: An evaluation of repeatability, intra-assay precision and capability to detect structural change. J Pharm Biomed Anal. 2016;123:162-72 pubmed publisher
    ..A combination of limited proteolysis and CD or SDS-PAGE analysis was shown to enhance the capacity of these techniques to detect structural change, which otherwise would not have been observed. ..
  44. Ortega Roldan J, Ossa F, Schnell J. Characterization of the human sigma-1 receptor chaperone domain structure and binding immunoglobulin protein (BiP) interactions. J Biol Chem. 2013;288:21448-57 pubmed publisher
  45. Nilsson M, Dobson C. In vitro characterization of lactoferrin aggregation and amyloid formation. Biochemistry. 2003;42:375-82 pubmed
    ..We suggest that such a process could be generally important in the formation of amyloid fibrils in vivo since the identification of both full-length protein and protein fragments is common in ex vivo amyloid deposits. ..
  46. Kailas L, Terry C, ABBOTT N, Taylor R, Mullin N, Tzokov S, et al. Surface architecture of endospores of the Bacillus cereus/anthracis/thuringiensis family at the subnanometer scale. Proc Natl Acad Sci U S A. 2011;108:16014-9 pubmed publisher
  47. Allen S, Lu H, Thornton D, Tokatlidis K. Juxtaposition of the two distal CX3C motifs via intrachain disulfide bonding is essential for the folding of Tim10. J Biol Chem. 2003;278:38505-13 pubmed
    ..Taken together these data reveal this specific intramolecular disulfide bonding as the crucial mechanism for Tim10 folding and show that the inner cysteine pair has a more prominent role in this process. ..
  48. Clark E, East J, Lee A. The role of tryptophan residues in an integral membrane protein: diacylglycerol kinase. Biochemistry. 2003;42:11065-73 pubmed
    ..As well as any structural effects, the presence of Trp residues in DGK has a clear effect on thermal stability. ..
  49. Wallis R, Shaw J, Uitdehaag J, Chen C, Torgersen D, Drickamer K. Localization of the serine protease-binding sites in the collagen-like domain of mannose-binding protein: indirect effects of naturally occurring mutations on protease binding and activation. J Biol Chem. 2004;279:14065-73 pubmed
    ..Thus, the effect of the mutations is to destabilize the collagen-like domain, indirectly disrupting the binding sites for MASPs. In addition, at least one of the mutations has a further effect on the ability of MBP to activate MASPs. ..
  50. Cusdin F, Nietlispach D, Maman J, Dale T, Powell A, Clare J, et al. The sodium channel {beta}3-subunit induces multiphasic gating in NaV1.3 and affects fast inactivation via distinct intracellular regions. J Biol Chem. 2010;285:33404-12 pubmed publisher
    ..The complex gating behavior induced by ?3 may contribute to the known hyperexcitability of peripheral neurons under those physiological conditions where expression of ?3 and Na(v)1.3 are both enhanced. ..
  51. Moreno F, Maldonado B, Wellner N, Mills E. Thermostability and in vitro digestibility of a purified major allergen 2S albumin (Ses i 1) from white sesame seeds (Sesamum indicum L.). Biochim Biophys Acta. 2005;1752:142-53 pubmed
  52. Ang S, Lu H. Deciphering structural and functional roles of individual disulfide bonds of the mitochondrial sulfhydryl oxidase Erv1p. J Biol Chem. 2009;284:28754-61 pubmed publisher
    ..Thus, we provided the first experimental evidence of electron transfer between the shuttle and active site disulfides of Erv1p, and we propose that both intersubunit and intermolecular electron transfer can occur. ..
  53. Rickman C, Jimenez J, Graham M, Archer D, Soloviev M, Burgoyne R, et al. Conserved prefusion protein assembly in regulated exocytosis. Mol Biol Cell. 2006;17:283-94 pubmed
  54. Seward H, Roujeinikova A, McLean K, Munro A, Leys D. Crystal structure of the Mycobacterium tuberculosis P450 CYP121-fluconazole complex reveals new azole drug-P450 binding mode. J Biol Chem. 2006;281:39437-43 pubmed
  55. Werbeck N, Rowling P, Chellamuthu V, Itzhaki L. Shifting transition states in the unfolding of a large ankyrin repeat protein. Proc Natl Acad Sci U S A. 2008;105:9982-7 pubmed publisher
  56. Ilioaia C, Johnson M, Horton P, Ruban A. Induction of efficient energy dissipation in the isolated light-harvesting complex of Photosystem II in the absence of protein aggregation. J Biol Chem. 2008;283:29505-12 pubmed publisher
    ..The implications of these findings for proposed mechanisms of energy dissipation in the Photosystem II antenna are discussed. ..
  57. Hu N, Bradshaw J, Lauter H, Buckingham J, Solito E, Hofmann A. Membrane-induced folding and structure of membrane-bound annexin A1 N-terminal peptides: implications for annexin-induced membrane aggregation. Biophys J. 2008;94:1773-81 pubmed
    ..The results are consistent with the hypothesis that the N-terminal domain of annexin A1 can serve as a secondary membrane binding site in the process of membrane aggregation by providing a peripheral membrane anchor. ..
  58. van Wonderen J, Knight C, Oganesyan V, George S, Zumft W, Cheesman M. Activation of the cytochrome cd1 nitrite reductase from Paracoccus pantotrophus. Reaction of oxidized enzyme with substrate drives a ligand switch at heme c. J Biol Chem. 2007;282:28207-15 pubmed
    ..This reactivity toward nitrite is also observed for oxidized cytochrome cd(1) from Pseudomonas stutzeri suggesting a more general involvement of the EPR-silent Fe(III) heme d(1) species in nitrite reduction...
  59. Siligardi G, Panaretou B, Meyer P, Singh S, Woolfson D, Piper P, et al. Regulation of Hsp90 ATPase activity by the co-chaperone Cdc37p/p50cdc37. J Biol Chem. 2002;277:20151-9 pubmed
    ..However, unlike Sti1/Hop/p60, which can displace geldanamycin upon binding to Hsp90, Cdc37p/p50(cdc37) forms a stable complex with geldanamycin-bound Hsp90 and may be sequestered in geldanamycin-inhibited Hsp90 complexes in vivo. ..
  60. Daltrop O, Ferguson S. Cytochrome c maturation. The in vitro reactions of horse heart apocytochrome c and Paracoccus dentrificans apocytochrome c550 with heme. J Biol Chem. 2003;278:4404-9 pubmed
    ..In vitro reaction of these apocytochromes c with heme to yield holocytochromes c, and the tendency to form a disulfide, have implications for the different systems responsible for cytochrome c maturation in vivo in various organisms. ..
  61. Brindley A, Raux E, Leech H, Schubert H, Warren M. A story of chelatase evolution: identification and characterization of a small 13-15-kDa "ancestral" cobaltochelatase (CbiXS) in the archaea. J Biol Chem. 2003;278:22388-95 pubmed publisher
    ..A classification scheme for chelatases is proposed...
  62. Hunt J, Beavil R, Calvert R, Gould H, Sutton B, Beavil A. Disulfide linkage controls the affinity and stoichiometry of IgE Fcepsilon3-4 binding to FcepsilonRI. J Biol Chem. 2005;280:16808-14 pubmed
  63. Vial S, Lu H, Allen S, Savory P, Thornton D, Sheehan J, et al. Assembly of Tim9 and Tim10 into a functional chaperone. J Biol Chem. 2002;277:36100-8 pubmed
    ..These data demonstrate that the individual subunits can exist as independent, dynamically self-associating proteins. Assembly into the thermodynamically stable hexameric complex is necessary for the TIM10 chaperone function. ..
  64. Arold S, Ulmer T, Mulhern T, Werner J, Ladbury J, Campbell I, et al. The role of the Src homology 3-Src homology 2 interface in the regulation of Src kinases. J Biol Chem. 2001;276:17199-205 pubmed
    ..Consequently, the interaction shows no enhancement by an avidity effect. Such an interaction may have evolved to gain specificity through an extended recognition site while maintaining rapid dissociation after signaling. ..
  65. Ciani B, Bjelic S, Honnappa S, Jawhari H, Jaussi R, Payapilly A, et al. Molecular basis of coiled-coil oligomerization-state specificity. Proc Natl Acad Sci U S A. 2010;107:19850-5 pubmed publisher
    ..They therefore should have major implications for the rational design of coiled coils and consequently many applications using these popular oligomerization domains. ..
  66. Jones C, Abdelraheim S, Brown D, Viles J. Preferential Cu2+ coordination by His96 and His111 induces beta-sheet formation in the unstructured amyloidogenic region of the prion protein. J Biol Chem. 2004;279:32018-27 pubmed
    ..The role of Cu(2+) in prion misfolding and disease must now be re-evaluated in the light of these findings. ..