Experts and Doctors on brucellosis in Argentina


Locale: Argentina
Topic: brucellosis

Top Publications

  1. Bukata L, Altabe S, de Mendoza D, Ugalde R, Comerci D. Phosphatidylethanolamine synthesis is required for optimal virulence of Brucella abortus. J Bacteriol. 2008;190:8197-203 pubmed publisher
    ..These results suggest that membrane phospholipid composition is critical for the interaction of B. abortus with the host cell. ..
  2. Lucero N, Escobar G, Ayala S, Silva Paulo P, Nielsen K. Fluorescence polarization assay for diagnosis of human brucellosis. J Med Microbiol. 2003;52:883-7 pubmed
    ..abortus, B. melitensis and B. suis and for identifying patients suffering relapses. Because of the ease of the procedure, it could be readily adopted for use in clinical laboratories and blood banks. ..
  3. Del Giudice M, Döhmer P, Spera J, Laporte F, Marchesini M, Czibener C, et al. VirJ Is a Brucella Virulence Factor Involved in the Secretion of Type IV Secreted Substrates. J Biol Chem. 2016;291:12383-93 pubmed publisher
    ..We hypothesize that VirJ is part of a secretion platform composed of the translocon and several secretion substrates and that it probably coordinates the proper assembly of this macromolecular complex. ..
  4. Roset M, Ciocchini A, Ugalde R, Iñón de Iannino N. Molecular cloning and characterization of cgt, the Brucella abortus cyclic beta-1,2-glucan transporter gene, and its role in virulence. Infect Immun. 2004;72:2263-71 pubmed
    ..These results suggest that cyclic beta-1,2-glucan must be transported into the periplasmatic space to exert its action as a virulence factor. ..
  5. Ruiz Ranwez V, Posadas D, Estein S, Abdian P, Martin F, Zorreguieta A. The BtaF trimeric autotransporter of Brucella suis is involved in attachment to various surfaces, resistance to serum and virulence. PLoS ONE. 2013;8:e79770 pubmed publisher
    ..Interestingly, BtaF was also implicated in the resistance of B. suis to porcine serum. Our findings emphasize the impact of TAs in the Brucella lifecycle. ..
  6. Cassataro J, Pasquevich K, Estein S, Laplagne D, Zwerdling A, de la Barrera S, et al. A DNA vaccine coding for the chimera BLSOmp31 induced a better degree of protection against B. ovis and a similar degree of protection against B. melitensis than Rev.1 vaccination. Vaccine. 2007;25:5958-67 pubmed
    ..Hitherto, this is the first indication that a recombinant subunit vaccine elicits greater protection than whole Brucella. ..
  7. Baldi P, Giambartolomei G, Wallach J, Velikovsky C, Fossati C. Limited diagnostic usefulness of antibodies to cytoplasmic proteins of Brucella in early-treated human brucellosis. Scand J Infect Dis. 2001;33:200-5 pubmed
    ..Given the higher specificity of the former and the higher sensitivity of the latter, both reactivities should be measured in order to diagnose human brucellosis. ..
  8. Czibener C, Del Giudice M, Spera J, Fulgenzi F, Ugalde J. Delta-pgm, a new live-attenuated vaccine against Brucella suis. Vaccine. 2016;34:1524-1530 pubmed publisher
    ..This novel strain could be an excellent candidate for the control of swine brucellosis, a disease of emerging concern in many parts of the world. ..
  9. Delpino M, Barrionuevo P, Scian R, Fossati C, Baldi P. Brucella-infected hepatocytes mediate potentially tissue-damaging immune responses. J Hepatol. 2010;53:145-54 pubmed publisher
    ..abortus infection and also by stimulation with supernatants from Brucella-infected neutrophils. The present study provides clues regarding potential mechanisms of tissue damage during liver brucellosis. ..

More Information


  1. Rossetti O, Arese A, Boschiroli M, Cravero S. Cloning of Brucella abortus gene and characterization of expressed 26-kilodalton periplasmic protein: potential use for diagnosis. J Clin Microbiol. 1996;34:165-9 pubmed
    ..BP26 might be of value as an antigen for serological diagnosis of brucellosis in different mammals. ..
  2. Baldi P, Araj G, Racaro G, Wallach J, Fossati C. Detection of antibodies to Brucella cytoplasmic proteins in the cerebrospinal fluid of patients with neurobrucellosis. Clin Diagn Lab Immunol. 1999;6:756-9 pubmed
    ..These findings suggest that, in addition to its usefulness in the serological diagnosis of human systemic brucellosis, the ELISA with CP antigen can be used for the specific diagnosis of human neurobrucellosis. ..
  3. Fernández A, Ferrero M, Hielpos M, Fossati C, Baldi P. Proinflammatory Response of Human Trophoblastic Cells to Brucella abortus Infection and upon Interactions with Infected Phagocytes. Biol Reprod. 2016;94:48 pubmed publisher
    ..abortus infections either through a direct response to the pathogen or through interactions with monocytes/macrophages or neutrophils, potentially contributing to the pregnancy complications of brucellosis. ..
  4. Velásquez L, Delpino M, Ibañez A, Coria L, Miraglia M, Scian R, et al. Brucella abortus induces apoptosis of human T lymphocytes. Microbes Infect. 2012;14:639-50 pubmed publisher
    ..Overall, these results represent a new mechanism whereby B. abortus by directly inhibiting T cell-mediated responses may evade adaptive immune responses. ..
  5. Wanke M. Canine brucellosis. Anim Reprod Sci. 2004;82-83:195-207 pubmed
    ..Infected animals must be removed from the kennels and no longer used for breeding. Preferably, males should be castrated and females spayed. Human contagion is not frequent, although it has been reported, and is easily treated. ..
  6. Miraglia M, Scian R, Samartino C, Barrionuevo P, Rodriguez A, Ibañez A, et al. Brucella abortus induces TNF-?-dependent astroglial MMP-9 secretion through mitogen-activated protein kinases. J Neuroinflammation. 2013;10:47 pubmed publisher
    ..abortus in astrocytes would lead to the production of MMP-9 and that MAPK may play a role in this phenomenon. MAPK inhibition may thus be considered as a strategy to control inflammation and CNS damage in neurobrucellosis. ..
  7. Pasquevich K, Estein S, García Samartino C, Samartino C, Zwerdling A, Coria L, et al. Immunization with recombinant Brucella species outer membrane protein Omp16 or Omp19 in adjuvant induces specific CD4+ and CD8+ T cells as well as systemic and oral protection against Brucella abortus infection. Infect Immun. 2009;77:436-45 pubmed publisher
    ..Overall these results indicate that U-Omp16 or U-Omp19 would be a useful candidate for a subunit vaccine against human and animal brucellosis. ..
  8. Milillo M, Velásquez L, Trotta A, Delpino M, Marinho F, Balboa L, et al. B. abortus RNA is the component involved in the down-modulation of MHC-I expression on human monocytes via TLR8 and the EGFR pathway. PLoS Pathog. 2017;13:e1006527 pubmed publisher
    ..Thus, bacteria can hide within infected cells and avoid the immunological surveillance of cytotoxic CD8+ T cells. ..
  9. Samartino L. Brucellosis in Argentina. Vet Microbiol. 2002;90:71-80 pubmed
    ..All Brucella species have been found in the country. Human brucellosis is an important disease and a national coordinated diagnostic net has been formed to better control the disease in man...
  10. Alvarez L, García Effrón G, Robles C. Identification of Brucella ovis exclusive genes in field isolates from Argentina. Vet J. 2016;209:196-8 pubmed publisher
    ..pinnipedialis. The results demonstrate that BOPI-1 is the only exclusive genetic region of B. ovis and marine Brucella spp. and that it is highly conserved in B. ovis field isolates from Argentina. ..
  11. Wallach J, Giambartolomei G, Baldi P, Fossati C. Human infection with M- strain of Brucella canis. Emerg Infect Dis. 2004;10:146-8 pubmed
    ..While this strain is avirulent in dogs, we report the case of clinical brucellosis that developed in a laboratory worker a few days after handling live M- cells for antigen production...
  12. Manias V, Nagel A, Mollerach A, Mendosa M, Freyre H, Gómez A, et al. [Brucella canis endocarditis: first documented case in Argentina]. Rev Argent Microbiol. 2013;45:50-3 pubmed
    ..The clinical significance of this case report lies in the possible failure of the empiric antibiotic therapy administered for endocarditis, since B. canis did not respond to the conventional antimicrobial treatment for this pathology...
  13. Carrica M, Fernandez I, Sieira R, Paris G, Goldbaum F. The two-component systems PrrBA and NtrYX co-ordinately regulate the adaptation of Brucella abortus to an oxygen-limited environment. Mol Microbiol. 2013;88:222-33 pubmed publisher
    ..abortus. The proposed regulatory network may contribute to understand the mechanisms used by Brucella for a successful adaptation to its replicative niche inside mammalian cells...
  14. Delpino M, Barrionuevo P, Macedo G, Oliveira S, Genaro S, Scian R, et al. Macrophage-elicited osteoclastogenesis in response to Brucella abortus infection requires TLR2/MyD88-dependent TNF-? production. J Leukoc Biol. 2012;91:285-98 pubmed publisher
    ..Our results indicate that B. abortus, through its lipoproteins, may be involved in bone resorption through the pathological induction of osteoclastogenesis...
  15. Marchesini M, Herrmann C, Salcedo S, Gorvel J, Comerci D. In search of Brucella abortus type IV secretion substrates: screening and identification of four proteins translocated into host cells through VirB system. Cell Microbiol. 2011;13:1261-74 pubmed publisher
    ..We confirmed VirB-mediated translocation of one of the substrates by immunofluorescence confocal microscopy, and we found that the N-terminal 25 amino acids are required for its delivery into cells...
  16. Pasquevich K, Ibañez A, Coria L, García Samartino C, Estein S, Zwerdling A, et al. An oral vaccine based on U-Omp19 induces protection against B. abortus mucosal challenge by inducing an adaptive IL-17 immune response in mice. PLoS ONE. 2011;6:e16203 pubmed publisher
    ..They also indicate different and important new aspects i) IL-17 does not contribute to reduce the bacterial burden in non vaccinated mice and ii) IL-17 plays a central role in vaccine mediated anti-Brucella mucosal immunity. ..
  17. Lucero N, Maldonado P, Kaufman S, Escobar G, Boeri E, Jacob N. Brucella canis causing infection in an HIV-infected patient. Vector Borne Zoonotic Dis. 2010;10:527-9 pubmed publisher
    ..To our knowledge, no other case of B. canis in the setting of HIV infection has been reported in the literature, and the emerging zoonotic potential of the disease in urban areas should be considered...
  18. Escobar G, Boeri E, Ayala S, Lucero N. The feasibility of using antigens prepared with rough Brucella strains for diagnosis of canine brucellosis. Rev Argent Microbiol. 2010;42:35-40 pubmed publisher
    ..RSAT is a very sensitive screening test and IELISA-B. canis, B. ovis and B. abortus RB51 could be used as confirmatory tests, since they show good specificity and sensitivity...
  19. Pasquevich K, García Samartino C, Coria L, Estein S, Zwerdling A, Ibañez A, et al. The protein moiety of Brucella abortus outer membrane protein 16 is a new bacterial pathogen-associated molecular pattern that activates dendritic cells in vivo, induces a Th1 immune response, and is a promising self-adjuvanting vaccine against syste. J Immunol. 2010;184:5200-12 pubmed publisher
  20. Wanke M, Delpino M, Baldi P. Use of enrofloxacin in the treatment of canine brucellosis in a dog kennel (clinical trial). Theriogenology. 2006;66:1573-8 pubmed
    ..We inferred that enrofloxacin could be used as an alternative drug for the treatment of canine brucellosis...
  21. Bonomi H, Marchesini M, Klinke S, Ugalde J, Zylberman V, Ugalde R, et al. An atypical riboflavin pathway is essential for Brucella abortus virulence. PLoS ONE. 2010;5:e9435 pubmed publisher
    ..In summary, we show that riboflavin biosynthesis is essential for Brucella survival inside cells or in mice. These results highlight the potential use of flavin biosynthetic pathway enzymes as targets for the chemotherapy of brucellosis...
  22. Reynes E, LOPEZ G, Ayala S, Hunter G, Lucero N. Monitoring infected dogs after a canine brucellosis outbreak. Comp Immunol Microbiol Infect Dis. 2012;35:533-7 pubmed publisher
    ..In an effort to protect the public, stray dogs should be controlled and educational programs about the risk of this zoonotic disease should be implemented...
  23. Giambartolomei G, Scian R, Acosta Rodríguez E, Fossati C, Delpino M. Brucella abortus-infected macrophages modulate T lymphocytes to promote osteoclastogenesis via IL-17. Am J Pathol. 2012;181:887-96 pubmed publisher
    ..Our results indicate that activated T cells, elicited by B. abortus-infected macrophages and influenced by the inflammatory milieu, promote the generation of osteoclasts, leading to bone loss...
  24. Cassataro J, Pasquevich K, Estein S, Laplagne D, Velikovsky C, de la Barrera S, et al. A recombinant subunit vaccine based on the insertion of 27 amino acids from Omp31 to the N-terminus of BLS induced a similar degree of protection against B. ovis than Rev.1 vaccination. Vaccine. 2007;25:4437-46 pubmed
  25. Paolicchi F, Núñez M, Fiorentino M, Malena R, Trangoni M, Cravero S, et al. [Immune response and reproductive consequences in experimentally infected ewes with Brucella ovis during late pregnancy]. Rev Argent Microbiol. 2013;45:13-20 pubmed
    ..Placenta colonization and milk excretion of B. ovis involves a potential source of infection for lambs, which could play a role as latent carriers of infection...
  26. Lucero N, Escobar G, Ayala S, Jacob N. Diagnosis of human brucellosis caused by Brucella canis. J Med Microbiol. 2005;54:457-61 pubmed
    ..canis human brucellosis, and a supplementary technique, such as IELISA, performed on all positive RSAT samples that were negative by B. abortus antigen could ensure diagnostic specificity and confirm the diagnosis...
  27. Wallach J, Ferrero M, Victoria Delpino M, Fossati C, Baldi P. Occupational infection due to Brucella abortus S19 among workers involved in vaccine production in Argentina. Clin Microbiol Infect. 2008;14:805-7 pubmed publisher
    ..abortus S19 among workers from vaccine-manufacturing plants, which in many cases was acquired from unnoticed exposures. Measures to improve the safety of B. abortus S19 handling should be implemented...
  28. Lucero N, Jacob N, Ayala S, Escobar G, Tuccillo P, Jacques I. Unusual clinical presentation of brucellosis caused by Brucella canis. J Med Microbiol. 2005;54:505-8 pubmed
    ..canis serology. The infection should be suspected in patients with compatible symptoms and negative serology for B. abortus antigen...
  29. Delpino M, Estein S, Fossati C, Baldi P. Partial protection against Brucella infection in mice by immunization with nonpathogenic alphaproteobacteria. Clin Vaccine Immunol. 2007;14:1296-301 pubmed
    ..anthropi, confers partial protection against Brucella challenge. However, such protection is lower than that conferred by immunization with whole Brucella or its cytosoluble fraction. ..
  30. Cassataro J, Velikovsky C, de la Barrera S, Estein S, Bruno L, Bowden R, et al. A DNA vaccine coding for the Brucella outer membrane protein 31 confers protection against B. melitensis and B. ovis infection by eliciting a specific cytotoxic response. Infect Immun. 2005;73:6537-46 pubmed
    ..The protective response could be related to the induction of CD8(+) T cells that eliminate Brucella-infected cells via the perforin pathway...
  31. Díaz A, Quinteros D, Llabot J, Palma S, Allemandi D, Ghersi G, et al. Spray dried microspheres based on chitosan: A promising new carrier for intranasal administration of polymeric antigen BLSOmp31 for prevention of ovine brucellosis. Mater Sci Eng C Mater Biol Appl. 2016;62:489-96 pubmed publisher
    ..Intranasal immunization induced systemic and local antibodies. In conclusion, the use of BLSOmp31-loaded chitosan spray-drying microspheres offers a promising way for nasal mucosal vaccination in sheep against brucellosis. ..
  32. Velikovsky C, Goldbaum F, Cassataro J, Estein S, Bowden R, Bruno L, et al. Brucella lumazine synthase elicits a mixed Th1-Th2 immune response and reduces infection in mice challenged with Brucella abortus 544 independently of the adjuvant formulation used. Infect Immun. 2003;71:5750-5 pubmed
  33. Baldi P, Giambartolomei G. Pathogenesis and pathobiology of zoonotic brucellosis in humans. Rev Sci Tech. 2013;32:117-25 pubmed
    ..Thus, although the inflammatory phenomena triggered by brucellae are relatively mild, they are long-lasting as a result of the prolonged intracellular persistence of the bacteria in infected tissues and eventually lead to tissue damage...
  34. Díaz A, Clausse M, Paolicchi F, Fiorentino M, Ghersi G, Zylberman V, et al. Immune response and serum bactericidal activity against Brucella ovis elicited using a short immunization schedule with the polymeric antigen BLSOmp31 in rams. Vet Immunol Immunopathol. 2013;154:36-41 pubmed publisher
    ..In the present work we evaluated and characterized, along 56 weeks after the first immunization of adult rams, the evolution of the immune response elicited by BLSOmp31 using a short immunization schedule. ..
  35. Lucero N, Corazza R, Almuzara M, Reynes E, Escobar G, Boeri E, et al. Human Brucella canis outbreak linked to infection in dogs. Epidemiol Infect. 2010;138:280-5 pubmed publisher
    ..The emerging zoonotic potential of this disease in urban areas and the need to coordinate canine brucellosis surveillance systems should be evaluated...
  36. Ruiz Ranwez V, Posadas D, Van der Henst C, Estein S, Arocena G, Abdian P, et al. BtaE, an adhesin that belongs to the trimeric autotransporter family, is required for full virulence and defines a specific adhesive pole of Brucella suis. Infect Immun. 2013;81:996-1007 pubmed publisher
    ..This is consistent with the inherent polarization of this bacterium, and its role in the invasion process...
  37. Baldi P, Giambartolomei G. Immunopathology of Brucella infection. Recent Pat Antiinfect Drug Discov. 2013;8:18-26 pubmed
    ..The article presents some of the recent patents related to such approaches...
  38. Scian R, Barrionuevo P, Giambartolomei G, De Simone E, Vanzulli S, Fossati C, et al. Potential role of fibroblast-like synoviocytes in joint damage induced by Brucella abortus infection through production and induction of matrix metalloproteinases. Infect Immun. 2011;79:3619-32 pubmed publisher
    ..These results suggest that FLS, and phagocytes recruited by them to the infection focus, may be involved in joint damage during brucellar arthritis through the production of MMPs and proinflammatory mediators...
  39. Lopez G, Ayala S, Escobar G, Lucero N. Use of Brucella canis antigen for detection of ovine serum antibodies against Brucella ovis. Vet Microbiol. 2005;105:181-7 pubmed
    ..ovis. The IELISA-B. canis or IELISA-B. ovis could be used as confirmatory tests, since they show equal specificity and sensitivity...