Genomes and Genes
Experts and Doctors on binding sites in Italy
Topic: binding sites
Publications291 found, 100 shown here
- Monti E, Preti A, Nesti C, Ballabio A, Borsani G. Expression of a novel human sialidase encoded by the NEU2 gene. Glycobiology. 1999;9:1313-21 pubmed..In addition, based on the detectable similarities between the NEU2 amino acid sequence and bacterial sialidases, a prediction of the three-dimensional structure of the enzyme was carried out using a protein homology modeling approach. ..
- Carro M, Spiga F, Quarto M, Di Ninni V, Volorio S, Alcalay M, et al. DEK Expression is controlled by E2F and deregulated in diverse tumor types. Cell Cycle. 2006;5:1202-7 pubmed..Expression levels of DEK in human tumors have been investigated by tissue micro array analysis. We find that DEK is overexpressed in many solid tumors such as colon cancer, larynx cancer, bladder cancer, and melanoma. ..
- Grazini U, Zanardi F, Citterio E, Casola S, Goding C, McBlane F. The RING domain of RAG1 ubiquitylates histone H3: a novel activity in chromatin-mediated regulation of V(D)J joining. Mol Cell. 2010;37:282-93 pubmed publisher..The results reveal that RAG1-mediated histone monoubiquitylation activity plays a role in regulating the joining phase of chromosomal V(D)J recombination. ..
- Federici L, Masulli M, Di Ilio C, Allocati N. Characterization of the hydrophobic substrate-binding site of the bacterial beta class glutathione transferase from Proteus mirabilis. Protein Eng Des Sel. 2010;23:743-50 pubmed publisher..This work paves the way for future studies aimed at improving the catalytic properties of beta class GSH transferases against selected substrates for bioremediation purposes...
- Aluigi M, Diaspro A, Ramoino P, Russo P, Falugi C. The sea urchin, Paracentrotus lividus, as a model to investigate the onset of molecules immunologically related to the ?-7 subunit of nicotinic receptors during embryonic and larval development. Curr Drug Targets. 2012;13:587-93 pubmed..The localization of these molecules paves the way to a speculation on their function and possible role in neurogenesis as well as neurodegeneration. ..
- Todone F, Vanoni M, Mozzarelli A, Bolognesi M, Coda A, Curti B, et al. Active site plasticity in D-amino acid oxidase: a crystallographic analysis. Biochemistry. 1997;36:5853-60 pubmed..Thus, in addition to an adaptable substrate binding site, DAAO has the ability to bind a highly distorted cofactor. This ability is relevant for the enzyme's function as a highly efficient oxidase. ..
- Cazzola M, Bergamaschi G, Tonon L, Arbustini E, Grasso M, Vercesi E, et al. Hereditary hyperferritinemia-cataract syndrome: relationship between phenotypes and specific mutations in the iron-responsive element of ferritin light-chain mRNA. Blood. 1997;90:814-21 pubmed..From a practical standpoint, this new genetic disorder should be taken into account by clinicians when facing a high serum ferritin in an apparently healthy person. ..
- Mattevi A, Tedeschi G, Bacchella L, Coda A, Negri A, Ronchi S. Structure of L-aspartate oxidase: implications for the succinate dehydrogenase/fumarate reductase oxidoreductase family. Structure. 1999;7:745-56 pubmed..Thus, LASPO, SDH and FRD form a class of functionally and structurally related oxidoreductases that are all able to reduce fumarate and to oxidise a dicarboxylate substrate. ..
- Amodeo P, Castiglione Morelli M, Strazzullo G, Fucile P, Gautel M, Motta A. Kinase recognition by calmodulin: modeling the interaction with the autoinhibitory region of human cardiac titin kinase. J Mol Biol. 2001;306:81-95 pubmed
- Bonomi F, Fessas D, Iametti S, Kurtz D, Mazzini S. Thermal stability of Clostridium pasteurianum rubredoxin: deconvoluting the contributions of the metal site and the protein. Protein Sci. 2000;9:2413-26 pubmed..The results indicate that thermally induced unfolding of the native Cp Rd must surmount a significant kinetic barrier caused by stabilizing interactions both within the protein and within the M(SCys)4 site. ..
- Demarchi F, Bertoli C, Sandy P, Schneider C. Glycogen synthase kinase-3 beta regulates NF-kappa B1/p105 stability. J Biol Chem. 2003;278:39583-90 pubmed..Interestingly, the increased sensitiveness to TNF-alpha-induced death occurring in GSK-3 beta-/- fibroblasts, which is coupled to a perturbation of p50/105 ratio, can be reproduced by p105 silencing in wild-type fibroblasts. ..
- Camera P, Da Silva J, Griffiths G, Giuffrida M, Ferrara L, Schubert V, et al. Citron-N is a neuronal Rho-associated protein involved in Golgi organization through actin cytoskeleton regulation. Nat Cell Biol. 2003;5:1071-8 pubmed..Together with recent data on endosomal dynamics, our results highlight the importance of organelle-specific Rho modulators for actin-dependent organelle organization and dynamics. ..
- Leonardi A, Barlocco D, Montesano F, Cignarella G, Motta G, Testa R, et al. Synthesis, screening, and molecular modeling of new potent and selective antagonists at the alpha 1d adrenergic receptor. J Med Chem. 2004;47:1900-18 pubmed..The most potent and selective compounds for alpha(1d) adrenergic receptor, i.e., 69 (Rec 26D/038) and 128 (Rec 26D/073), are characterized by the presence of the 2,5-dichlorophenylpiperazine moiety. ..
- Sturiale L, Barone R, Fiumara A, Perez M, Zaffanello M, Sorge G, et al. Hypoglycosylation with increased fucosylation and branching of serum transferrin N-glycans in untreated galactosemia. Glycobiology. 2005;15:1268-76 pubmed..We also demonstrated that in galactosemia patients, transferrin N-glycan biosynthesis is restored upon dietary treatment. ..
- Rizzuti B, Sportelli L, Guzzi R. Structural, dynamical and functional aspects of the inner motions in the blue copper protein azurin. Biophys Chem. 2007;125:532-9 pubmed..A number of other structural and dynamic features of azurin are discussed in the context of the blue copper protein family and an explanation is proposed to account for the variability/conservation of some regions in the cupredoxins. ..
- Bartucci R, Guzzi R, De Zotti M, Toniolo C, Sportelli L, Marsh D. Backbone dynamics of alamethicin bound to lipid membranes: spin-echo electron paramagnetic resonance of TOAC-spin labels. Biophys J. 2008;94:2698-705 pubmed..Associated fluctuations in polar fields from the peptide could facilitate ion permeation. ..
- Cappelli A, Nannicini C, Valenti S, Giuliani G, Anzini M, Mennuni L, et al. Design, synthesis, and preliminary biological evaluation of pyrrolo[3,4-c]quinolin-1-one and oxoisoindoline derivatives as aggrecanase inhibitors. ChemMedChem. 2010;5:739-48 pubmed publisher..On the other hand, the oxoisoindoline derivatives 3 a,b lack significant ADAMTS-4 inhibitory activity and inhibit ADAMTS-5 showing IC(25) values in the micromolar range. ..
- Maurelli S, Chiesa M, Giamello E, Di Nardo G, Ferrero V, Gilardi G, et al. Direct spectroscopic evidence for binding of anastrozole to the iron heme of human aromatase. Peering into the mechanism of aromatase inhibition. Chem Commun (Camb). 2011;47:10737-9 pubmed publisher..Using hyperfine sublevel correlation (HYSCORE) spectroscopy we provide the first experimental evidence of the binding of anastrozole to the iron heme of human aromatase. ..
- Repetto D, Aramu S, Boeri Erba E, Sharma N, Grasso S, Russo I, et al. Mapping of p140Cap phosphorylation sites: the EPLYA and EGLYA motifs have a key role in tyrosine phosphorylation and Csk binding, and are substrates of the Abl kinase. PLoS ONE. 2013;8:e54931 pubmed publisher..Elucidating the function of p140Cap will provide novel insights into its biological activity not only in normal cells, but also in tumors. ..
- Schiaffino Ortega S, Baglioni E, Mariotto E, Bortolozzi R, Serrán Aguilera L, Ríos Marco P, et al. Design, synthesis, crystallization and biological evaluation of new symmetrical biscationic compounds as selective inhibitors of human Choline Kinase ?1 (ChoK?1). Sci Rep. 2016;6:23793 pubmed publisher..Moreover, the crystal structure of ChoK?1 with compound 10a reveals that this compound binds to the choline-binding site and mimics HC-3 binding mode as never before. ..
- Tripepi S, Carelli A, Perrotta E, Brunelli E, Tavolaro R, Facciolo R, et al. Morphological and functional variations of Leydig cells in testis of the domestic pig during the different biological stages of development. J Exp Zool. 2000;287:167-75 pubmed
- Colnaghi R, Cassinelli G, Drummond M, Forlani F, Pagani S. Properties of the Escherichia coli rhodanese-like protein SseA: contribution of the active-site residue Ser240 to sulfur donor recognition. FEBS Lett. 2001;500:153-6 pubmed..On the contrary, substitution of Ser240 with an ionizable residue (Lys) increased the affinity for thiosulfate. ..
- Cora D, Herrmann C, Dieterich C, Di Cunto F, Provero P, Caselle M. Ab initio identification of putative human transcription factor binding sites by comparative genomics. BMC Bioinformatics. 2005;6:110 pubmed..The method is based on three ingredients: comparative genomics, overrepresentation, different types of coregulation. The method is applied to a full-scan of the human genome, giving satisfactory results. ..
- Perri A, Vizza D, Lupinacci S, Toteda G, De Amicis F, Leone F, et al. Adiponectin secreted by tubular renal cells during LPS exposure worsens the cellular inflammatory damage. J Nephrol. 2016;29:185-194 pubmed publisher..Collectively our results suggest that in HK-2 cells, ADPN produced upon LPS stimulus could worsen the inflammatory damage in an autocrine-dependent manner. ..
- Bolognesi M, Rosano C, Losso R, Borassi A, Rizzi M, Wittenberg J, et al. Cyanide binding to Lucina pectinata hemoglobin I and to sperm whale myoglobin: an x-ray crystallographic study. Biophys J. 1999;77:1093-9 pubmed..The unusual cyanide distal site orientation observed in L. pectinata HbI, at room temperature, may reflect reduction of the heme Fe(III) atom induced by free radical species during x-ray data collection using Cu Kalpha radiation. ..
- Meola A, Sbardellati A, Bruni Ercole B, Cerretani M, Pezzanera M, Ceccacci A, et al. Binding of hepatitis C virus E2 glycoprotein to CD81 does not correlate with species permissiveness to infection. J Virol. 2000;74:5933-8 pubmed..These results indicate that the binding of E2 to CD81 is not predictive of an infection-producing interaction between HCV and host cells. ..
- Esposito L, Sica F, Raia C, Giordano A, Rossi M, Mazzarella L, et al. Crystal structure of the alcohol dehydrogenase from the hyperthermophilic archaeon Sulfolobus solfataricus at 1.85 A resolution. J Mol Biol. 2002;318:463-77 pubmed publisher..This latter metal ion shows a peculiar coordination, with a glutamic acid residue replacing one of the four cysteine ligands that are highly conserved throughout the structural zinc-containing dimeric ADHs...
- Poerio E, Di Gennaro S, Di Maro A, Farisei F, Ferranti P, Parente A. Primary structure and reactive site of a novel wheat proteinase inhibitor of subtilisin and chymotrypsin. Biol Chem. 2003;384:295-304 pubmed publisher..The wheat inhibitor WSCI has a single reactive site (the peptide bond between methionyl-48 and glutamyl-49 residues) as identified by affinity chromatography and sequence analysis...
- Bernat A, Avvakumov N, Mymryk J, Banks L. Interaction between the HPV E7 oncoprotein and the transcriptional coactivator p300. Oncogene. 2003;22:7871-81 pubmed..Finally, we show that E7 can abolish the p300-mediated E2 transactivation function, suggesting that complex formation between E7 and p300 may contribute to the regulation of E2 transcriptional activity. ..
- Bonsegna S, Slocombe S, De Bellis L, Baker A. AtLACS7 interacts with the TPR domains of the PTS1 receptor PEX5. Arch Biochem Biophys. 2005;443:74-81 pubmed
- Mattioli L, Anelli T, Fagioli C, Tacchetti C, Sitia R, Valetti C. ER storage diseases: a role for ERGIC-53 in controlling the formation and shape of Russell bodies. J Cell Sci. 2006;119:2532-41 pubmed..Our findings identify a novel ERGIC-53 substrate, and indicate that interactions with light chains or ERGIC-53 seed muDeltaCH1 condensation in different stations of the early secretory pathway. ..
- Careri M, Elviri L, Mangia A, Zagnoni I, Torta F, Cavazzini D, et al. Mass spectrometry techniques for detection of ligand-dependent changes in the conformational flexibility of cellular retinol-binding protein type I localized by hydrogen/deuterium exchange. Rapid Commun Mass Spectrom. 2006;20:1973-80 pubmed
- Ricchiuto P, Rocco A, Gianazza E, Corrada D, Beringhelli T, Eberini I. Structural and dynamic roles of permanent water molecules in ligand molecular recognition by chicken liver bile acid binding protein. J Mol Recognit. 2008;21:348-54 pubmed publisher..In cL-BABP, the interactions between this conserved water molecule and the three residues are present in the crystal of both apo- and holo-cL-BABP but are lost immediately after the start of molecular dynamics. ..
- Perrera C, Colombo R, Valsasina B, Carpinelli P, Troiani S, Modugno M, et al. Identification of Myb-binding protein 1A (MYBBP1A) as a novel substrate for aurora B kinase. J Biol Chem. 2010;285:11775-85 pubmed publisher..The results presented herein show MYBBP1A as a novel Aurora B kinase substrate and reveal a not yet recognized link of this nucleolar protein to mitosis. ..
- D Addabbo P, Scascitelli M, Giambra V, Rocchi M, Frezza D. Position and sequence conservation in Amniota of polymorphic enhancer HS1.2 within the palindrome of IgH 3'Regulatory Region. BMC Evol Biol. 2011;11:71 pubmed publisher..2 show the relevance of these structures in the control and modulation of the Ig production through the formation of possible three-dimensional structures. ..
- Mura M, Cappato S, Giacopelli F, Ravazzolo R, Bocciardi R. The role of the 3'UTR region in the regulation of the ACVR1/Alk-2 gene expression. PLoS ONE. 2012;7:e50958 pubmed publisher..Considering that no specific and effective treatment of FOP is available, clarifying the basic mechanisms of the ACVR1/Alk-2 gene biology may provide means to develop innovative therapeutics approaches. ..
- di Salvo M, Remesh S, Vivoli M, Ghatge M, Paiardini A, D Aguanno S, et al. On the catalytic mechanism and stereospecificity of Escherichia coli L-threonine aldolase. FEBS J. 2014;281:129-45 pubmed publisher..Substrate recognition and reaction specificity seem to be guided by the overall microenvironment that surrounds the substrate at the enzyme active site, rather than by one ore more specific residues. ..
- Battiston L, Passamonti S, Macagno A, Sottocasa G. The bilirubin-binding motif of bilitranslocase and its relation to conserved motifs in ancient biliproteins. Biochem Biophys Res Commun. 1998;247:687-92 pubmed..From these experiments a dissociation constant of 2.2 +/- 0.3 and 11.3 +/- 1.3 nM for bilirubin-bilitranslocase and nicotinate-bilitranslocase complexes were calculated. ..
- Ponzetto C, Pante G, Prunotto C, Ieraci A, Maina F. Met signaling mutants as tools for developmental studies. Int J Dev Biol. 2000;44:645-53 pubmed..Mice expressing a Met receptor with partial loss of signaling function survived until birth and revealed novel aspects of HGF/SF-Met function during muscle development. ..
- Fassio A, Merlo D, Mapelli J, Menegon A, Corradi A, Mete M, et al. The synapsin domain E accelerates the exoendocytotic cycle of synaptic vesicles in cerebellar Purkinje cells. J Cell Sci. 2006;119:4257-68 pubmed..These effects may increase the sensitivity of postsynaptic neurons to inhibition and thereby contribute to the inhibitory control of network activity. ..
- Siglioccolo A, Bossa F, Pascarella S. Structural adaptation of serine hydroxymethyltransferase to low temperatures. Int J Biol Macromol. 2010;46:37-46 pubmed publisher..In general, the property variations show a coherent opposite trend in the hyperthermophilic population: in particular, increase of hydrophobicity, packing and decrease of flexibility was observed. ..
- Falconi M, Rotilio G, Desideri A. Modelling the three-dimensional structure and electrostatic potential field of the two Cu,Zn superoxide dismutase variants from Xenopus laevis. Proteins. 1991;10:149-55 pubmed
- Petersen B, Lukas J, Sørensen C, Bartek J, Helin K. Phosphorylation of mammalian CDC6 by cyclin A/CDK2 regulates its subcellular localization. EMBO J. 1999;18:396-410 pubmed..Based on our data we suggest that the phosphorylation of CDC6 by Cyclin A/CDK2 is a negative regulatory event that could be implicated in preventing re-replication during S phase and G2. ..
- Melck D, De Petrocellis L, Orlando P, Bisogno T, Laezza C, Bifulco M, et al. Suppression of nerve growth factor Trk receptors and prolactin receptors by endocannabinoids leads to inhibition of human breast and prostate cancer cell proliferation. Endocrinology. 2000;141:118-26 pubmed..These findings suggest that endogenous cannabinoids and CB1 receptor agonists are potential negative effectors of PRL- and NGF-induced biological responses, at least in some cancer cells. ..
- Cervoni L, Lascu I, Xu Y, Gonin P, Morr M, Merouani M, et al. Binding of nucleotides to nucleoside diphosphate kinase: a calorimetric study. Biochemistry. 2001;40:4583-9 pubmed..Titration showed the stoichiometry of the interaction to be abnormal, with 9--12 binding sites/hexamer. The presence of supplementary binding sites might have physiological implications. ..
- Schiering N, Knapp S, Marconi M, Flocco M, Cui J, Perego R, et al. Crystal structure of the tyrosine kinase domain of the hepatocyte growth factor receptor c-Met and its complex with the microbial alkaloid K-252a. Proc Natl Acad Sci U S A. 2003;100:12654-9 pubmed..K-252a is bound in the adenosine pocket with an analogous binding mode to those observed in previously reported structures of protein kinases in complex with staurosporine. ..
- Gabbianelli R, D Orazio M, Pacello F, O Neill P, Nicolini L, Rotilio G, et al. Distinctive functional features in prokaryotic and eukaryotic Cu,Zn superoxide dismutases. Biol Chem. 2004;385:749-54 pubmed..We suggest that differences between individual bacterial variants could be due to the influence of modifications at the dimer interface on the enzyme conformational flexibility. ..
- Benfante R, Antonini R, Vaccari M, Flora A, Chen F, Clementi F, et al. The expression of the human neuronal alpha3 Na+,K+-ATPase subunit gene is regulated by the activity of the Sp1 and NF-Y transcription factors. Biochem J. 2005;386:63-72 pubmed..Although the expression of these factors is not tissue-specific, co-operative functional interactions among them are required to direct the activity of the promoter predominantly in neuronal cells. ..
- Di Pierro E, Cappellini M, Mazzucchelli R, Moriondo V, Mologni D, Zanone Poma B, et al. A point mutation affecting an SP1 binding site in the promoter of the ferrochelatase gene impairs gene transcription and causes erythropoietic protoporphyria. Exp Hematol. 2005;33:584-91 pubmed..This is the first report of a mutation in the FECH promoter affecting binding of a transcription factor and causing EPP phenotype. ..
- Van Der Stelt M, Di Marzo V. Anandamide as an intracellular messenger regulating ion channel activity. Prostaglandins Other Lipid Mediat. 2005;77:111-22 pubmed..Here, we will review the evidence for this hypothesis. ..
- Cilia E, Fabbri A, Uriani M, Scialdone G, Ammendola S. The signature amidase from Sulfolobus solfataricus belongs to the CX3C subgroup of enzymes cleaving both amides and nitriles. Ser195 and Cys145 are predicted to be the active site nucleophiles. FEBS J. 2005;272:4716-24 pubmed..The results of random and site-directed mutagenesis experiments, as well as inhibition studies support our hypothesis. ..
- Andreotti G, Méndez B, Amodeo P, Morelli M, Nakamuta H, Motta A. Structural determinants of salmon calcitonin bioactivity: the role of the Leu-based amphipathic alpha-helix. J Biol Chem. 2006;281:24193-203 pubmed..Taken together, the structural and biological data identify well defined structural parameters of the helix for sCT bioactivity. ..
- Bottomley M, Lo Surdo P, Di Giovine P, Cirillo A, Scarpelli R, Ferrigno F, et al. Structural and functional analysis of the human HDAC4 catalytic domain reveals a regulatory structural zinc-binding domain. J Biol Chem. 2008;283:26694-704 pubmed publisher..HDAC3 repressor complex. Together, these data suggest a key role of the structural zinc-binding domain in the regulation of class IIa HDAC functions. ..
- Cigliano L, D Andrea L, Maresca B, Serino M, Carlucci A, Salvatore A, et al. Relevance of the amino acid conversions L144R (Zaragoza) and L159P (Zavalla) in the apolipoprotein A-I binding site for haptoglobin. Biol Chem. 2008;389:1421-6 pubmed publisher..Therefore, both amino acid conversions affect haptoglobin binding and LCAT regulation. We highlight the role of haptoglobin in LCAT regulation in subjects with ApoA-I variants. ..
- La Regina G, Silvestri R, Gatti V, Lavecchia A, Novellino E, Befani O, et al. Synthesis, structure-activity relationships and molecular modeling studies of new indole inhibitors of monoamine oxidases A and B. Bioorg Med Chem. 2008;16:9729-40 pubmed publisher..However, only compound 22 was able to form hydrogen bonds with FAD, a finding which was in accordance with its potent anti-MAO-A activity. Conversely, 22/MAOB complex was highly unstable during the MD simulation. ..
- Gardini A, Cesaroni M, Luzi L, Okumura A, Biggs J, Minardi S, et al. AML1/ETO oncoprotein is directed to AML1 binding regions and co-localizes with AML1 and HEB on its targets. PLoS Genet. 2008;4:e1000275 pubmed publisher..Our results suggest a major role for the functional interaction of AML1/ETO with AML1 and HEB in transcriptional regulation determined by the fusion protein. ..
- Dani N, Stilla A, Marchegiani A, Tamburro A, Till S, Ladurner A, et al. Combining affinity purification by ADP-ribose-binding macro domains with mass spectrometry to define the mammalian ADP-ribosyl proteome. Proc Natl Acad Sci U S A. 2009;106:4243-8 pubmed publisher..This represents an important step toward the discovery of new ADP-ribosyltransferase targets and an understanding of the physiological role and the pharmacological potential of this protein modification. ..
- Zifarelli G, Liantonio A, Gradogna A, Picollo A, Gramegna G, De Bellis M, et al. Identification of sites responsible for the potentiating effect of niflumic acid on ClC-Ka kidney chloride channels. Br J Pharmacol. 2010;160:1652-61 pubmed publisher..Our results are an important step towards the development of ClC-Ka activators for treating Bartter syndrome types III and IV with residual channel activity. ..
- Brigati C, Banelli B, Casciano I, di Vinci A, Matis S, Cutrona G, et al. Epigenetic mechanisms regulate ?NP73 promoter function in human tonsil B cells. Mol Immunol. 2011;48:408-14 pubmed publisher..Our results suggest a ?Np73 function in B cell immunity, indicate epigenetics as master TP73 P2 regulator, and point to AP1 site occupancy as playing an putative mechanistic role in this process. ..
- Tran C, Giangrossi M, Prosseda G, Brandi A, Di Martino M, Colonna B, et al. A multifactor regulatory circuit involving H-NS, VirF and an antisense RNA modulates transcription of the virulence gene icsA of Shigella flexneri. Nucleic Acids Res. 2011;39:8122-34 pubmed publisher..Bases engaged in the initial contact leading to sense-antisense pairing have been identified using synthetic RNA and DNA oligonucleotides designed to rebuild and mutagenize the two stem-loop motifs of the antisense region...
- Marchetti R, Malinovská L, Lameignere E, Adamova L, De Castro C, Cioci G, et al. Burkholderia cenocepacia lectin A binding to heptoses from the bacterial lipopolysaccharide. Glycobiology. 2012;22:1387-98 pubmed publisher..Additionally, the specific binding of fluorescent BC2L-A lectin on B. cenocepacia bacterial surface was demonstrated by microscopy. ..
- Di Leva F, Festa C, D Amore C, De Marino S, Renga B, D Auria M, et al. Binding mechanism of the farnesoid X receptor marine antagonist suvanine reveals a strategy to forestall drug modulation on nuclear receptors. Design, synthesis, and biological evaluation of novel ligands. J Med Chem. 2013;56:4701-17 pubmed publisher..Deep pharmacological evaluation demonstrated that derivative 19 represents a new chemotype of FXR modulator, whereas alcohol 6, with a simplified molecular scaffold, exhibits excellent antagonistic activity. ..
- Panza S, Santoro M, De Amicis F, Morelli C, Passarelli V, D Aquila P, et al. Estradiol via estrogen receptor beta influences ROS levels through the transcriptional regulation of SIRT3 in human seminoma TCam-2 cells. Tumour Biol. 2017;39:1010428317701642 pubmed publisher..Future studies may lead to additional strategies for the current therapy of seminoma. ..
- Fagioli M, Alcalay M, Pandolfi P, Venturini L, Mencarelli A, Simeone A, et al. Alternative splicing of PML transcripts predicts coexpression of several carboxy-terminally different protein isoforms. Oncogene. 1992;7:1083-91 pubmed..RNAase protection experiments revealed that the different PML isoforms are equally expressed in established cell lines of different histological origin. ..
- Condorelli G, Constanzo L, De Guidi G, Giuffrida S, Sortino S. Molecular mechanism of drug photosensitization. 7. Photocleavage of DNA sensitized by suprofen. Photochem Photobiol. 1995;62:155-61 pubmed..The experimental techniques used for assessing the photodamaging activity of this drug may be useful for screening of phototoxic compounds in the environment and for determining the active species involved. ..
- Bergamini C, Signorini M, Caselli L, Melandri P. Regulation of transglutaminase activity by GTP in digitonin permeabilized Yoshida tumor cells. Biochem Mol Biol Int. 1993;30:727-32 pubmed..GTP inhibits protein labelling at low calcium concentration by inhibiting type 2 TGase without affecting membrane-bound TGase. These results support the notion that inhibition of type 2 TGase by GTP is physiologically relevant...
- Miloso M, Limauro D, Alifano P, Rivellini F, Lavitola A, Gulletta E, et al. Characterization of the rho genes of Neisseria gonorrhoeae and Salmonella typhimurium. J Bacteriol. 1993;175:8030-7 pubmed..The function of the Rho factor of N. gonorrhoeae was investigated by complementation assays of rho mutants of E. coli and S. typhimurium and by in vivo transcription assays in polar mutants of S. typhimurium. ..
- Zara V, Dietmeier K, Palmisano A, Vozza A, Rassow J, Palmieri F, et al. Yeast mitochondria lacking the phosphate carrier/p32 are blocked in phosphate transport but can import preproteins after regeneration of a membrane potential. Mol Cell Biol. 1996;16:6524-31 pubmed
- Bellorini M, Zemzoumi K, Farina A, Berthelsen J, Piaggio G, Mantovani R. Cloning and expression of human NF-YC. Gene. 1997;193:119-25 pubmed..We evaluated the expression of NF-YC and found that mRNA levels are similar in different human tissues except in testis. ..
- Vigo E, Muller H, Prosperini E, Hateboer G, Cartwright P, Moroni M, et al. CDC25A phosphatase is a target of E2F and is required for efficient E2F-induced S phase. Mol Cell Biol. 1999;19:6379-95 pubmed..Taken together, our results provide an important step in defining how E2F activity leads to deregulated proliferation. ..
- Dieci G, Giuliodori S, Catellani M, Percudani R, Ottonello S. Intragenic promoter adaptation and facilitated RNA polymerase III recycling in the transcription of SCR1, the 7SL RNA gene of Saccharomyces cerevisiae. J Biol Chem. 2002;277:6903-14 pubmed
- Faniello M, Chirico G, Quaresima B, Cuda G, Allevato G, Bevilacqua M, et al. An alternative model of H ferritin promoter transactivation by c-Jun. Biochem J. 2002;363:53-8 pubmed..These results add a new level of complexity to transcriptional regulation by c-Jun, which can activate p300-dependent promoters without binding directly to the target DNA. ..
- Occhipinti E, Martelli P, Spinozzi F, Corsi F, Formantici C, Molteni L, et al. 3D structure of Sulfolobus solfataricus carboxypeptidase developed by molecular modeling is confirmed by site-directed mutagenesis and small angle X-ray scattering. Biophys J. 2003;85:1165-75 pubmed..The fitting procedure showed that only the model built using the Pseudomonas carboxypeptidase G2 structure as a template fitted the experimental data. ..
- Accardi A, Pusch M. Conformational changes in the pore of CLC-0. J Gen Physiol. 2003;122:277-93 pubmed..These observations suggest that channel opening in CLC-0 consists in more than a movement of a side chain and that other parts of the channel and of the selectivity filter are probably involved...
- Ferraroni M, Solyanikova I, Kolomytseva M, Scozzafava A, Golovleva L, Briganti F. Crystal structure of 4-chlorocatechol 1,2-dioxygenase from the chlorophenol-utilizing gram-positive Rhodococcus opacus 1CP. J Biol Chem. 2004;279:27646-55 pubmed..The present structure is the first of intradiol dioxygenases that specifically catalyze the cleavage of chlorocatechols, key intermediates in the aerobic catabolism of toxic chloroaromatics. ..
- Cirillo P, Cali G, Golino P, Calabro P, Forte L, De Rosa S, et al. Tissue factor binding of activated factor VII triggers smooth muscle cell proliferation via extracellular signal-regulated kinase activation. Circulation. 2004;109:2911-6 pubmed
- de Sanctis D, Dewilde S, Vonrhein C, Pesce A, Moens L, Ascenzi P, et al. Bishistidyl heme hexacoordination, a key structural property in Drosophila melanogaster hemoglobin. J Biol Chem. 2005;280:27222-9 pubmed..Despite the high resolution achieved, no sequence/structure features specifically supporting the heme hexa- to pentacoordination transition required for diatomic ligand binding could be recognized. ..
- Bolognese F, Pitarque Martì M, Lo Cicero V, Mantovani R, Maier J. Characterization of the human EDF-1 minimal promoter: involvement of NFY and Sp1 in the regulation of basal transcription. Gene. 2006;374:87-95 pubmed..Deletion of Sp1/Sp3 and NF-Y consensus sequences resulted in the total loss of EDF-1 promoter activity. Our studies indicate that Sp1 and NFY binding is essential for EDF-1 promoter activity. ..
- Manca L, Pirastru M, Mereu P, Multineddu C, Olianas A, el Sherbini E, et al. Barbary sheep (Ammotragus lervia): the structure of the adult beta-globin gene and the functional properties of its hemoglobin. Comp Biochem Physiol B Biochem Mol Biol. 2006;145:214-9 pubmed
- Dall Osto L, Cazzaniga S, North H, Marion Poll A, Bassi R. The Arabidopsis aba4-1 mutant reveals a specific function for neoxanthin in protection against photooxidative stress. Plant Cell. 2007;19:1048-64 pubmed..Neoxanthin appears particularly active against superoxide anions produced by the Mehler's reaction, whose rate is known to be enhanced in abiotic stress conditions. ..
- Innocenti A, Scozzafava A, Supuran C. Carbonic anhydrase inhibitors. Inhibition of transmembrane isoforms IX, XII, and XIV with less investigated anions including trithiocarbonate and dithiocarbamate. Bioorg Med Chem Lett. 2010;20:1548-50 pubmed publisher..12-0.66mM). N,N-Diethyldithiocarbamate was a low micromolar inhibitor also against CA XII and XIV (K(I)s of 1.0-1.1microM), suggesting that this new zinc-binding group (CS2-) may lead to efficient inhibitors targeting transmembrane CAs...
- Cichero E, Fossa P. Docking-based 3D-QSAR analyses of pyrazole derivatives as HIV-1 non-nucleoside reverse transcriptase inhibitors. J Mol Model. 2012;18:1573-82 pubmed publisher..723, SEE?=?0.248, F?=?240.291, and r(2) (pred)?=?0.77. The results allowed us to obtain useful information for the design of new compounds with improved potency toward WT HIV-1 and also against clinically relevant resistant mutants. ..
- Cendron L, Berto P, D Adamo S, Vallese F, Govoni C, Posewitz M, et al. Crystal structure of HydF scaffold protein provides insights into [FeFe]-hydrogenase maturation. J Biol Chem. 2011;286:43944-50 pubmed publisher..The crystal structure of the latter furnishes several clues about the events necessary for cluster generation/transfer and provides an excellent model to begin elucidating the structure/function of HydF in [FeFe]-hydrogenase maturation...
- Baggiani C, Giovannoli C, Anfossi L, Passini C, Baravalle P, Giraudi G. A connection between the binding properties of imprinted and nonimprinted polymers: a change of perspective in molecular imprinting. J Am Chem Soc. 2012;134:1513-8 pubmed publisher..Moreover, for closely related molecules, it was shown that binding selectivity is an emergent property derived from the imprinting process and not a property of NIPs. ..