Genomes and Genes
Experts and Doctors on bacterial drug resistance in Sweden
Topic: bacterial drug resistance
- Lindback E, Gharizadeh B, Ataker F, Airell A, Jalal S, Nyrén P, et al. DNA gyrase gene in Neisseria gonorrhoeae as indicator for resistance to ciprofloxacin and species verification. Int J STD AIDS. 2005;16:142-7 pubmed..Our conclusion is that gyrA is an indicator of resistance to ciprofloxacin in N. gonorrhoeae and sequencing by Pyrosequencing technology is a suitable tool for analysis of DNA in urine samples. ..
- Huitric E, Verhasselt P, Andries K, Hoffner S. In vitro antimycobacterial spectrum of a diarylquinoline ATP synthase inhibitor. Antimicrob Agents Chemother. 2007;51:4202-4 pubmed..032 microg/ml. Out of 20 additional mycobacterial species, three were found to be naturally resistant to R207910 and were shown to exhibit a polymorphism in their atpE genes. ..
- Olofsson S, Geli P, Andersson D, Cars O. Pharmacodynamic model to describe the concentration-dependent selection of cefotaxime-resistant Escherichia coli. Antimicrob Agents Chemother. 2005;49:5081-91 pubmed
- Karlsson M, Aspan A, Landén A, Franklin A. Further characterization of porcine Brachyspira hyodysenteriae isolates with decreased susceptibility to tiamulin. J Med Microbiol. 2004;53:281-5 pubmed..No other co-resistance with tiamulin was found. ..
- Egervärn M, Lindmark H, Roos S, Huys G, Lindgren S. Effects of inoculum size and incubation time on broth microdilution susceptibility testing of lactic acid bacteria. Antimicrob Agents Chemother. 2007;51:394-6 pubmed..An increase in either parameter resulted in elevated MICs for all species. An inoculum of 3 x 10(5) CFU/ml is recommended to assess the antibiotic susceptibilities of these bacteria by using broth microdilution. ..
- Holm B, Petersson U, Mörner A, Bergstrom K, Franklin A, Greko C. Antimicrobial resistance in staphylococci from canine pyoderma: a prospective study of first-time and recurrent cases in Sweden. Vet Rec. 2002;151:600-5 pubmed..No resistance to penicillinase-stable beta-lactams was observed. A comparison with earlier studies indicated that there had been a marked increase in resistance during the previous five years. ..
- Kugelberg E, Lofmark S, Wretlind B, Andersson D. Reduction of the fitness burden of quinolone resistance in Pseudomonas aeruginosa. J Antimicrob Chemother. 2005;55:22-30 pubmed..Our results show that 'no cost' and compensatory mutations are common in quinolone-resistant P. aeruginosa. ..
- Sun S, Negrea A, Rhen M, Andersson D. Genetic analysis of colistin resistance in Salmonella enterica serovar Typhimurium. Antimicrob Agents Chemother. 2009;53:2298-305 pubmed publisher..The low fitness costs and the high mutation rate suggest that mutants with reduced susceptibility to colistin could emerge in clinical settings. ..
- Noren T, Wullt M, Akerlund T, Back E, Odenholt I, Burman L. Frequent emergence of resistance in Clostridium difficile during treatment of C. difficile-associated diarrhea with fusidic acid. Antimicrob Agents Chemother. 2006;50:3028-32 pubmed..Development of resistance in C. difficile was frequent in patients given fusidic acid, but it was without apparent negative impact on therapeutic efficacy in the actual CDAD episode. ..
- Lofmark S, Jernberg C, Jansson J, Edlund C. Clindamycin-induced enrichment and long-term persistence of resistant Bacteroides spp. and resistance genes. J Antimicrob Chemother. 2006;58:1160-7 pubmed..The aim was to study the long-term consequences of 1 week clindamycin administration regarding selection and persistence of resistance, resistance determinants and diversity of the Bacteroides spp. in the intestinal microflora...
- Pringle M, Landén A, Franklin A. Tiamulin resistance in porcine Brachyspira pilosicoli isolates. Res Vet Sci. 2006;80:1-4 pubmed..The relationship between the 13 tiamulin resistant isolates was analyzed by pulsed-field gel electrophoresis (PFGE). Among the 13 isolates 10 different PFGE patterns were identified...
- Nilsson A, Berg O, Aspevall O, Kahlmeter G, Andersson D. Biological costs and mechanisms of fosfomycin resistance in Escherichia coli. Antimicrob Agents Chemother. 2003;47:2850-8 pubmed..According to the mathematical model, the reduced growth rate of the resistant strains will prevent them from establishing in the bladder, which could explain why fosfomycin resistance remains rare in clinical isolates. ..
- Wrande M, Roth J, Hughes D. Accumulation of mutants in "aging" bacterial colonies is due to growth under selection, not stress-induced mutagenesis. Proc Natl Acad Sci U S A. 2008;105:11863-8 pubmed publisher..Evidence is presented that these Rif(R) mutants accumulate because they grow faster than parent cells during the aging period. Direct tests revealed no increase in the frequency of other mutant types during the aging period. ..
- Zorzet A, Pavlov M, Nilsson A, Ehrenberg M, Andersson D. Error-prone initiation factor 2 mutations reduce the fitness cost of antibiotic resistance. Mol Microbiol. 2010;75:1299-313 pubmed publisher..More generally, our results suggest that bacterial protein synthesis is mRNA-limited and that compensatory mutations in IF2 could increase the persistence of PDFI-resistant bacteria in clinical settings. ..
- Qvarnstr m Y, Swedberg G. Sulphonamide resistant commensal Neisseria with alterations in the dihydropteroate synthase can be isolated from carriers not exposed to sulphonamides. BMC Microbiol. 2002;2:34 pubmed..meningitidis. Sulphonamide resistant commensal Neisseria were isolated from an environment not exposed to sulphonamides, suggesting that resistant Neisseria has become a natural part of the commensal throat flora...
- Jonsson M, Swedberg G. Macrolide resistance can be transferred by conjugation from viridans streptococci to Streptococcus pyogenes. Int J Antimicrob Agents. 2006;28:101-3 pubmed..It therefore appears reasonable to draw the conclusion that this element is important in the lateral transfer of macrolide resistance between streptococci...
- Olofsson S, Marcusson L, Strömbäck A, Hughes D, Cars O. Dose-related selection of fluoroquinolone-resistant Escherichia coli. J Antimicrob Chemother. 2007;60:795-801 pubmed..coli mutants. Thus, among approved fluoroquinolone dosing regimens, some are significantly more effective than others in exceeding the mutant selection window and preventing the enrichment of resistant mutants. ..
- Chromek M, Slamová Z, Bergman P, Kovacs L, Podracká L, Ehren I, et al. The antimicrobial peptide cathelicidin protects the urinary tract against invasive bacterial infection. Nat Med. 2006;12:636-41 pubmed..In addition, clinical E. coli strains that were more resistant to LL-37 caused more severe urinary tract infections than did susceptible strains. Thus, cathelicidin seems to be a key factor in mucosal immunity of the urinary tract. ..
- Nord C, Sillerström E, Wahlund E. Effect of tigecycline on normal oropharyngeal and intestinal microflora. Antimicrob Agents Chemother. 2006;50:3375-80 pubmed..05) but no impact on bacteroides. No Clostridium difficile strains were isolated. Two Klebsiella pneumoniae strains and five Enterobacter cloacae strains resistant to tigecycline were found on day 8. ..
- Borjesson S, Dienues O, Jarnheimer P, Olsen B, Matussek A, Lindgren P. Quantification of genes encoding resistance to aminoglycosides, beta-lactams and tetracyclines in wastewater environments by real-time PCR. Int J Environ Health Res. 2009;19:219-30 pubmed publisher..The developed LUX real-time PCR assays were shown to be fast and reproducible tools for detection and quantification of the four genes encoding antibiotic resistance in wastewater. ..
- Dahlen G, Blomquist S, Carlen A. A retrospective study on the microbiology in patients with oral complaints and oral mucosal lesions. Oral Dis. 2009;15:265-72 pubmed publisher..Systematic evaluation of different treatment strategies is needed. ..
- Alsterholm M, Flytström I, Bergbrant I, Faergemann J. Fusidic acid-resistant Staphylococcus aureus in impetigo contagiosa and secondarily infected atopic dermatitis. Acta Derm Venereol. 2010;90:52-7 pubmed publisher..FRSA levels were persistently low in AD. Continued restrictive use of topical fusidic acid is advised to limit an increase in FRSA levels in dermatology patients...
- Demoling L, Bååth E. No long-term persistence of bacterial pollution-induced community tolerance in tylosin-polluted soil. Environ Sci Technol. 2008;42:6917-21 pubmed
- Lofmark S, Jernberg C, Billström H, Andersson D, Edlund C. Restored fitness leads to long-term persistence of resistant Bacteroides strains in the human intestine. Anaerobe. 2008;14:157-60 pubmed publisher..The results indicate that the biological cost associated with a resistance gene can rapidly be compensated during in vivo growth. Thus, once the resistant clone has gained its resistance determinant it will be difficult to eliminate. ..
- Rapp M, Seppälä S, Granseth E, von Heijne G. Emulating membrane protein evolution by rational design. Science. 2007;315:1282-4 pubmed..This simple evolutionary pathway can explain the frequent occurrence of membrane proteins with an internal pseudo-two-fold symmetry axis in the plane of the membrane. ..
- Hägg P, de Pohl J, Abdulkarim F, Isaksson L. A host/plasmid system that is not dependent on antibiotics and antibiotic resistance genes for stable plasmid maintenance in Escherichia coli. J Biotechnol. 2004;111:17-30 pubmed..Also environmental problems caused by release of antibiotics and antibiotic resistance genes, that potentially can give horizontal gene transfer between bacterial populations, are eliminated. ..
- Seppälä S, Slusky J, Lloris Garcerá P, Rapp M, von Heijne G. Control of membrane protein topology by a single C-terminal residue. Science. 2010;328:1698-700 pubmed publisher..This observation points to an unanticipated plasticity in membrane protein insertion mechanisms. ..
- Matsson M, Hederstedt L. The carboxin-binding site on Paracoccus denitrificans succinate:quinone reductase identified by mutations. J Bioenerg Biomembr. 2001;33:99-105 pubmed..The block would be either by direct exclusion of ubiquinone from the active site or by occlusion of a pore that leads to the active site...
- Sandegren L, Lindqvist A, Kahlmeter G, Andersson D. Nitrofurantoin resistance mechanism and fitness cost in Escherichia coli. J Antimicrob Chemother. 2008;62:495-503 pubmed publisher..coli in the absence of antibiotic. In the presence of therapeutic levels of nitrofurantoin, even resistant mutants are so disturbed in growth that they are probably unable to become enriched and establish an infection. ..
- Paulander W, Maisnier Patin S, Andersson D. Multiple mechanisms to ameliorate the fitness burden of mupirocin resistance in Salmonella typhimurium. Mol Microbiol. 2007;64:1038-48 pubmed..Our findings show that the fitness costs of ileS mutations conferring mupirocin resistance can be reduced by several types of mechanisms that may contribute to the stability of mupirocin resistance in clinical settings. ..
- Pringle M, Fellström C, Johansson K. Decreased susceptibility to doxycycline associated with a 16S rRNA gene mutation in Brachyspira hyodysenteriae. Vet Microbiol. 2007;123:245-8 pubmed..In the B. hyodysenteriae sequences a G1058C mutation was found for all isolates with increased doxycycline MICs whereas all susceptible isolates had the wild type sequence...
- Lovmar M, Nilsson K, Vimberg V, Tenson T, Nervall M, Ehrenberg M. The molecular mechanism of peptide-mediated erythromycin resistance. J Biol Chem. 2006;281:6742-50 pubmed..These model predictions are compared with experimental observations from growing bacterial cultures, and excellent agreement is found between theoretical prediction and experimental observation. ..