Experts and Doctors on atp dependent proteases in Japan


Locale: Japan
Topic: atp dependent proteases

Top Publications

  1. Tojo N, Inouye S, Komano T. The lonD gene is homologous to the lon gene encoding an ATP-dependent protease and is essential for the development of Myxococcus xanthus. J Bacteriol. 1993;175:4545-9 pubmed
    ..xanthus was found to be arrested at an early stage in these strains. The mutant strains were able to form neither fruiting bodies nor myxospores. ..
  2. Yamaguchi Y, Tomoyasu T, Takaya A, Morioka M, Yamamoto T. Effects of disruption of heat shock genes on susceptibility of Escherichia coli to fluoroquinolones. BMC Microbiol. 2003;3:16 pubmed
    ..Our results suggest that agents capable of inhibiting the Lon protease have potential for combination therapy with FQs. ..
  3. Akiyama Y, Yoshihisa T, Ito K. FtsH, a membrane-bound ATPase, forms a complex in the cytoplasmic membrane of Escherichia coli. J Biol Chem. 1995;270:23485-90 pubmed
    ..We suggest that the N-terminal transmembrane region of FtsH mediates directly the interaction between the FtsH subunits. ..
  4. Kihara A, Akiyama Y, Ito K. FtsH is required for proteolytic elimination of uncomplexed forms of SecY, an essential protein translocase subunit. Proc Natl Acad Sci U S A. 1995;92:4532-6 pubmed
    ..The primary role of FtsH is discussed in light of the quite pleiotropic mutational effects, which now include stabilization of uncomplexed SecY. ..
  5. Akiyama Y, Shirai Y, Ito K. Involvement of FtsH in protein assembly into and through the membrane. II. Dominant mutations affecting FtsH functions. J Biol Chem. 1994;269:5225-9 pubmed
    ..It was suggested that the amino-terminal membrane region of FtsH interacts with other component(s), and that the two putative ATP binding sites are of vital importance for the FtsH functions. ..
  6. Tomoyasu T, Yamanaka K, Murata K, Suzaki T, Bouloc P, Kato A, et al. Topology and subcellular localization of FtsH protein in Escherichia coli. J Bacteriol. 1993;175:1352-7 pubmed
    ..The average number of FtsH molecules per cell was estimated to be approximately 400. ..
  7. Asahara Y, Atsuta K, Motohashi K, Taguchi H, Yohda M, Yoshida M. FtsH recognizes proteins with unfolded structure and hydrolyzes the carboxyl side of hydrophobic residues. J Biochem. 2000;127:931-7 pubmed
    ..FtsH, which lacked the N-terminal two transmembrane helices, was also prepared but was found to retain neither ATPase nor protease activities. Thus, the membrane segment appeared to be indispensable for these activities of T.FtsH. ..
  8. Qu J, Makino S, Adachi H, Koyama Y, Akiyama Y, Ito K, et al. The tolZ gene of Escherichia coli is identified as the ftsH gene. J Bacteriol. 1996;178:3457-61 pubmed
    ..The tolZ21 mutant was found to have a suppressor mutation, named sfhC, which allowed cells to survive. The sfhC mutation alone caused no Tol-, Nfc-, Ts, or Hfl- phenotypes in the tolZ21 mutant. ..
  9. Akiyama Y, Kihara A, Ito K. Subunit a of proton ATPase F0 sector is a substrate of the FtsH protease in Escherichia coli. FEBS Lett. 1996;399:26-8 pubmed
    ..These results suggest that FtsH serves as a quality-control mechanism to avoid potentially harmful accumulation of free subunit a in the membrane. ..

More Information


  1. Ogura T, Inoue K, Tatsuta T, Suzaki T, Karata K, Young K, et al. Balanced biosynthesis of major membrane components through regulated degradation of the committed enzyme of lipid A biosynthesis by the AAA protease FtsH (HflB) in Escherichia coli. Mol Microbiol. 1999;31:833-44 pubmed
    ..The biosynthesis of phospholipids and the lipid A moiety of lipopolysaccharide, both of which derive their fatty acyl chains from the same R-3-hydroxyacyl-ACP pool, is regulated by FtsH. ..
  2. Kihara A, Akiyama Y, Ito K. A protease complex in the Escherichia coli plasma membrane: HflKC (HflA) forms a complex with FtsH (HflB), regulating its proteolytic activity against SecY. EMBO J. 1996;15:6122-31 pubmed
    ..Finally, purified HflKC protein inhibited the SecY-degrading activity of purified FtsH protein in vitro. These results indicate that the proteolytic activity of FtsH is modulated negatively by its association with HflKC. ..
  3. Hori O, Ichinoda F, Tamatani T, Yamaguchi A, Sato N, Ozawa K, et al. Transmission of cell stress from endoplasmic reticulum to mitochondria: enhanced expression of Lon protease. J Cell Biol. 2002;157:1151-60 pubmed
  4. Inagawa T, Kato J, Niki H, Karata K, Ogura T. Defective plasmid partition in ftsH mutants of Escherichia coli. Mol Genet Genomics. 2001;265:755-62 pubmed
    ..It is likely that altered membrane structure affects the localization or activity of a putative plasmid partitioning apparatus located at positions equivalent to 1/4 and 3/4 of the cell length. ..
  5. Ishii Y, Amano F. Regulation of SulA cleavage by Lon protease by the C-terminal amino acid of SulA, histidine. Biochem J. 2001;358:473-80 pubmed
    ..These results suggest that the histidine residue at the extreme C-terminus of SulA is recognized specifically by Lon, leading to a high-affinity interaction between SulA and Lon...
  6. Ishii Y, Sonezaki S, Iwasaki Y, Miyata Y, Akita K, Kato Y, et al. Regulatory role of C-terminal residues of SulA in its degradation by Lon protease in Escherichia coli. J Biochem. 2000;127:837-44 pubmed
    ..These results show that SulA has the specified rows of C-terminal 8 residues recognized by Lon, leading to facilitated binding and subsequent cleavage by Lon protease both in vivo and in vitro...
  7. Akiyama Y, Ehrmann M, Kihara A, Ito K. Polypeptide binding of Escherichia coli FtsH (HflB). Mol Microbiol. 1998;28:803-12 pubmed
    ..Thus, denatured PhoA binding of FtsH may also occur in vivo...
  8. Kihara A, Akiyama Y, Ito K. Different pathways for protein degradation by the FtsH/HflKC membrane-embedded protease complex: an implication from the interference by a mutant form of a new substrate protein, YccA. J Mol Biol. 1998;279:175-88 pubmed
    ..From these results we suggest that there are at least two pathways for FtsH-dependent protein degradation, only one of which (probably for membrane proteins) is subject to the HflKC-dependent interference by the YccA11 mutant substrate. ..
  9. Kanemori M, Nishihara K, Yanagi H, Yura T. Synergistic roles of HslVU and other ATP-dependent proteases in controlling in vivo turnover of sigma32 and abnormal proteins in Escherichia coli. J Bacteriol. 1997;179:7219-25 pubmed
    ..Thus, a set of ATP-dependent proteases appear to play synergistic roles in the negative control of the heat shock response by modulating in vivo turnover of sigma32 as well as through degradation of abnormal proteins. ..
  10. Kitagawa M, Wada C, Yoshioka S, Yura T. Expression of ClpB, an analog of the ATP-dependent protease regulatory subunit in Escherichia coli, is controlled by a heat shock sigma factor (sigma 32). J Bacteriol. 1991;173:4247-53 pubmed
    ..coli. ..
  11. Shirai Y, Akiyama Y, Ito K. Suppression of ftsH mutant phenotypes by overproduction of molecular chaperones. J Bacteriol. 1996;178:1141-5 pubmed
    ..These results suggest that FtsH functions can be somehow compensated for when the cellular concentrations of some molecular chaperones increase. ..
  12. Watabe S, Kohno H, Kouyama H, Hiroi T, Yago N, Nakazawa T. Purification and characterization of a substrate protein for mitochondrial ATP-dependent protease in bovine adrenal cortex. J Biochem. 1994;115:648-54 pubmed
    ..Since a segment around AA47 was highly conserved, this residue may be important for the biochemical functions of SP-22. ..
  13. Tomoyasu T, Gamer J, Bukau B, Kanemori M, Mori H, Rutman A, et al. Escherichia coli FtsH is a membrane-bound, ATP-dependent protease which degrades the heat-shock transcription factor sigma 32. EMBO J. 1995;14:2551-60 pubmed
    ..We conclude that FtsH is a novel membrane-bound, ATP-dependent metalloprotease with activity for sigma 32. These findings indicate a new mechanism of gene regulation in E. coli. ..
  14. Akiyama Y, Ito K. A new Escherichia coli gene, fdrA, identified by suppression analysis of dominant negative FtsH mutations. Mol Gen Genet. 1995;249:202-8 pubmed
    ..The intact fdrA gene (11.9 min on the chromosome) directed the synthesis of a 60 kDa protein in vitro. ..
  15. Tomoyasu T, Yuki T, Morimura S, Mori H, Yamanaka K, Niki H, et al. The Escherichia coli FtsH protein is a prokaryotic member of a protein family of putative ATPases involved in membrane functions, cell cycle control, and gene expression. J Bacteriol. 1993;175:1344-51 pubmed
    ..g., Sec18p, Pas1p, CDC48p, and TBP-1, which function in protein transport pathways, peroxisome assembly, cell division cycle, and gene expression, respectively. Possible implications of these observations are discussed. ..