Genomes and Genes
Experts and Doctors on adenosine triphosphate in United Kingdom
Locale: United Kingdom
Topic: adenosine triphosphate
Publications218 found, 100 shown here
- Barrera N, Morales B, Villalon M. ATP and adenosine trigger the interaction of plasma membrane IP3 receptors with protein kinase A in oviductal ciliated cells. Biochem Biophys Res Commun. 2007;364:815-21 pubmed..We show here that activation of plasma membrane IP3 receptors directly triggers Ca2+ influx in response to ATP and that these receptors are modulated by adenosine-activated PKA. ..
- Niwa H, Ewens C, Tsang C, Yeung H, Zhang X, Freemont P. The role of the N-domain in the ATPase activity of the mammalian AAA ATPase p97/VCP. J Biol Chem. 2012;287:8561-70 pubmed publisher..This leads us to propose a model where the N-domain adopts either of two conformations: a flexible conformation compatible with ATP hydrolysis or a coplanar conformation that is inactive. ..
- Song D, Ashcroft F. ATP modulation of ATP-sensitive potassium channel ATP sensitivity varies with the type of SUR subunit. J Biol Chem. 2001;276:7143-9 pubmed..This suggests that MgATP may cause disruption of the cytoskeleton, leading to enhanced membrane phospholipid levels (or better targeting to the K(ATP) channel) and thus to decreased channel ATP sensitivity. ..
- Weitzer S, Lehane C, Uhlmann F. A model for ATP hydrolysis-dependent binding of cohesin to DNA. Curr Biol. 2003;13:1930-40 pubmed..After DNA replication, two DNA strands may be trapped to promote sister chromatid cohesion. In anaphase, Scc1 cleavage opens the ring to release sister chromatids. ..
- Webb M, Reid G, Munasinghe V, Corrie J. A series of related nucleotide analogues that aids optimization of fluorescence signals in probing the mechanism of P-loop ATPases, such as actomyosin. Biochemistry. 2004;43:14463-71 pubmed..The ability of this set of analogues to produce large signals indicated potential uses when scarce proteins are studied in small amounts. ..
- Haider S, Grottesi A, Hall B, Ashcroft F, Sansom M. Conformational dynamics of the ligand-binding domain of inward rectifier K channels as revealed by molecular dynamics simulations: toward an understanding of Kir channel gating. Biophys J. 2005;88:3310-20 pubmed..It is of interest that loss of exact rotational symmetry has also been suggested to play a role in gating in the bacterial Kir homolog, KirBac1.1, and in the nicotinic acetylcholine receptor channel. ..
- Pearson R, Dale N, Llaudet E, Mobbs P. ATP released via gap junction hemichannels from the pigment epithelium regulates neural retinal progenitor proliferation. Neuron. 2005;46:731-44 pubmed..ATP released from RPE hemichannels speeds both cell division and proliferation in the neural retina. ..
- Benítez Rajal J, Lorite M, Burt A, Day C, Thompson M. Phospholipase D and extracellular signal-regulated kinase in hepatic stellate cells: effects of platelet-derived growth factor and extracellular nucleotides. Am J Physiol Gastrointest Liver Physiol. 2006;291:G977-86 pubmed..Instead, these responses may be linked to other phenotypic changes associated with activated HSC such as increases in contraction, motility, or extracellular matrix deposition. ..
- Fyfe P, Oza S, Fairlamb A, Hunter W. Leishmania trypanothione synthetase-amidase structure reveals a basis for regulation of conflicting synthetic and hydrolytic activities. J Biol Chem. 2008;283:17672-80 pubmed publisher..The potential inhibitory role of the C terminus provides a mechanism to control relative levels of the critical metabolites, trypanothione, glutathionylspermidine, and spermidine in Leishmania. ..
- Sweeney T, Cisnetto V, Bose D, Bailey M, Wilson J, Zhang X, et al. Foot-and-mouth disease virus 2C is a hexameric AAA+ protein with a coordinated ATP hydrolysis mechanism. J Biol Chem. 2010;285:24347-59 pubmed publisher..Our results provide new insights into the structure and mechanism of picornavirus 2C proteins that will facilitate new investigations of their roles in infection. ..
- Kampranis S, Bates A, Maxwell A. A model for the mechanism of strand passage by DNA gyrase. Proc Natl Acad Sci U S A. 1999;96:8414-9 pubmed..This mechanism is concerted, in that capture of the transported segment by the ATP-operated clamp induces opening of the DNA gate, which in turn stimulates ATP hydrolysis. ..
- Braybrooke J, Spink K, Thacker J, Hickson I. The RAD51 family member, RAD51L3, is a DNA-stimulated ATPase that forms a complex with XRCC2. J Biol Chem. 2000;275:29100-6 pubmed..Given the requirements for XRCC2 in genetic recombination and protection against DNA-damaging agents, we suggest that the complex of RAD51L3 and XRCC2 is likely to be important for these functions in human cells. ..
- Storm J, O Mara M, Crowley E, Peall J, Tieleman D, Kerr I, et al. Residue G346 in transmembrane segment six is involved in inter-domain communication in P-glycoprotein. Biochemistry. 2007;46:9899-910 pubmed..Homology modeling of P-glycoprotein indicated that the G346C mutation caused a steric interaction between TM5 and TM6, thereby precluding a helical movement required to support ATP hydrolysis. ..
- Neame S, Rubin L, Philpott K. Blocking cytochrome c activity within intact neurons inhibits apoptosis. J Cell Biol. 1998;142:1583-93 pubmed..These data suggest that cytochrome c is an intrinsic but not limiting component of the neuronal apoptotic pathway. ..
- Carlin L, Eleme K, McCann F, Davis D. Intercellular transfer and supramolecular organization of human leukocyte antigen C at inhibitory natural killer cell immune synapses. J Exp Med. 2001;194:1507-17 pubmed..In addition, different arrangements of HLA-C are seen at inhibitory NK immune synapses, and these alter as NK synapses mature, but in a fashion distinct from that seen upon T cell activation. ..
- Ranson N, Farr G, Roseman A, Gowen B, Fenton W, Horwich A, et al. ATP-bound states of GroEL captured by cryo-electron microscopy. Cell. 2001;107:869-79 pubmed..The model for cooperativity, based on switching of intersubunit salt bridge interactions around the GroEL ring, may provide general insight into cooperativity in other ring complexes and molecular machines. ..
- Clarson L, Roberts V, Greenwood S, Elliott A. ATP-stimulated Ca(2+)-activated K(+) efflux pathway and differentiation of human placental cytotrophoblast cells. Am J Physiol Regul Integr Comp Physiol. 2002;282:R1077-85 pubmed..The dependency of this pathway on [Ca(2+)](o) is greater in the 66-h multinucleate syncytiotrophoblast-like cells, suggesting that the mechanism for Ca(2+) entry may be altered during differentiation of trophoblast cells. ..
- Flaus A, Owen Hughes T. Dynamic properties of nucleosomes during thermal and ATP-driven mobilization. Mol Cell Biol. 2003;23:7767-79 pubmed..The dynamic properties of nucleosomes we describe are likely to influence their role in gene regulation...
- Bowler M, Montgomery M, Leslie A, Walker J. How azide inhibits ATP hydrolysis by the F-ATPases. Proc Natl Acad Sci U S A. 2006;103:8646-9 pubmed..It also explains the stimulatory effect of azide on ATP-sensitive potassium channels by enhancing the binding of ADP. ..
- Doshi R, Ali A, Shi W, Freeman E, Fagg L, van Veen H. Molecular disruption of the power stroke in the ATP-binding cassette transport protein MsbA. J Biol Chem. 2013;288:6801-13 pubmed publisher..Our observations are the first to suggest that modulation of tetrahelix bundle interactions in ATP-binding cassette exporters might offer a potent strategy to alter their transport activity...
- Matic I, Cocco S, Ferraina C, Martin Jimenez R, Florenzano F, Crosby J, et al. Neuroprotective coordination of cell mitophagy by the ATPase Inhibitory Factor 1. Pharmacol Res. 2016;103:56-68 pubmed publisher..These data suggest that in mammalian neurons mitochondria adapt to respiratory stress by upregulating IF1, which exerts a protective role by coordinating pro-survival cell mitophagy and bioenergetics resilience. ..
- Schiffmann Y. The non-equilibrium basis of Turing Instability and localised biological work. Prog Biophys Mol Biol. 2017;127:12-32 pubmed publisher..The TC patterns and the bifurcation parameter manifest quantisation and symmetry as in music and in applications of quantum mechanics. The notion of correlation diagrams is also introduced. ..
- Mitchell K, Pinton P, Varadi A, Tacchetti C, Ainscow E, Pozzan T, et al. Dense core secretory vesicles revealed as a dynamic Ca(2+) store in neuroendocrine cells with a vesicle-associated membrane protein aequorin chimaera. J Cell Biol. 2001;155:41-51 pubmed..The presence of RyRs on secretory vesicles suggests that local Ca(2+)-induced Ca(2+) release from vesicles docked at the plasma membrane could participate in triggering exocytosis. ..
- Vial S, Lu H, Allen S, Savory P, Thornton D, Sheehan J, et al. Assembly of Tim9 and Tim10 into a functional chaperone. J Biol Chem. 2002;277:36100-8 pubmed..These data demonstrate that the individual subunits can exist as independent, dynamically self-associating proteins. Assembly into the thermodynamically stable hexameric complex is necessary for the TIM10 chaperone function. ..
- Ashton A, Ushkaryov Y. Properties of synaptic vesicle pools in mature central nerve terminals. J Biol Chem. 2005;280:37278-88 pubmed..Our findings also demonstrate that respective vesicle pools have similar characteristics and requirements in excitatory and inhibitory nerve terminals. ..
- Danahay H, Atherton H, Jackson A, Kreindler J, Poll C, Bridges R. Membrane capacitance and conductance changes parallel mucin secretion in the human airway epithelium. Am J Physiol Lung Cell Mol Physiol. 2006;290:L558-69 pubmed..Transepithelial impedance analysis offers the potential to study morphological and conductance changes in cultured human bronchial epithelial cells. ..
- Bradley H, Baldwin J, Goli G, Johnson B, Zou J, Sivaprasadarao A, et al. Residues 155 and 348 contribute to the determination of P2X7 receptor function via distinct mechanisms revealed by single-nucleotide polymorphisms. J Biol Chem. 2011;286:8176-87 pubmed publisher..Such interpretations are consistent with the locations of the residues in the structural model of the hP2X(7) receptor. ..
- Alkhaldi A, Martinek J, Panicucci B, Dardonville C, ZÃkovÃ¡ A, de Koning H. Trypanocidal action of bisphosphonium salts through a mitochondrial target in bloodstream form Trypanosoma brucei. Int J Parasitol Drugs Drug Resist. 2016;6:23-34 pubmed publisher..The results show that, in T. brucei, bisphosphonium compounds do not principally act on succinate dehydrogenase but on the mitochondrial FoF1 ATPase. ..
- Rossiter H, Ward S, Howe F, Wood D, Kowalchuk J, Griffiths J, et al. Effects of dichloroacetate on VO2 and intramuscular 31P metabolite kinetics during high-intensity exercise in humans. J Appl Physiol (1985). 2003;95:1105-15 pubmed
- Da Silva Xavier G, Rutter J, Rutter G. Involvement of Per-Arnt-Sim (PAS) kinase in the stimulation of preproinsulin and pancreatic duodenum homeobox 1 gene expression by glucose. Proc Natl Acad Sci U S A. 2004;101:8319-24 pubmed..We conclude that PASK is an important metabolic sensor in nutrient-sensitive mammalian cells and plays an unexpected role in the regulation of key genes involved in maintaining the differentiated phenotype of pancreatic beta cells. ..
- Hardie D. Sensing of energy and nutrients by AMP-activated protein kinase. Am J Clin Nutr. 2011;93:891S-6 pubmed publisher..Most of these agents activate AMPK because they inhibit mitochondrial function. ..
- Suleiman M, Dihmis W, Caputo M, Angelini G, Bryan A. Changes in myocardial concentration of glutamate and aspartate during coronary artery surgery. Am J Physiol. 1997;272:H1063-9 pubmed..This work documents the changes in the intracellular concentrations of important metabolites in the hearts of patients undergoing coronary artery surgery using different myocardial protection techniques...
- Allen N, Karadottir R, Attwell D. A preferential role for glycolysis in preventing the anoxic depolarization of rat hippocampal area CA1 pyramidal cells. J Neurosci. 2005;25:848-59 pubmed..They also define a hierarchy of pool sizes for hippocampal energy stores and suggest that brain ATP production from glial lactate may not be significant in conditions of energy deprivation. ..
- Evans A, Mustard K, Wyatt C, Peers C, Dipp M, Kumar P, et al. Does AMP-activated protein kinase couple inhibition of mitochondrial oxidative phosphorylation by hypoxia to calcium signaling in O2-sensing cells?. J Biol Chem. 2005;280:41504-11 pubmed..Thus, metabolic sensing by AMP-activated protein kinase may mediate chemotransduction by hypoxia. ..
- Johnson S, Kennedy H, Holley M, Fettiplace R, Marcotti W. The resting transducer current drives spontaneous activity in prehearing mammalian cochlear inner hair cells. J Neurosci. 2012;32:10479-83 pubmed publisher..We propose that, in vivo, spontaneous Ca(2+) action potentials are intrinsically generated by IHCs up to the onset of hearing and that they are likely to influence the final sensory-independent refinement of the developing cochlea. ..
- Cleeter M, Chau K, Gluck C, Mehta A, Hughes D, Duchen M, et al. Glucocerebrosidase inhibition causes mitochondrial dysfunction and free radical damage. Neurochem Int. 2013;62:1-7 pubmed publisher..We suggest that GBA mutations and reduced GCase activity may increase the risk for PD by inducing these same abnormalities in PD brain...
- Gartland A, Hipskind R, Gallagher J, Bowler W. Expression of a P2X7 receptor by a subpopulation of human osteoblasts. J Bone Miner Res. 2001;16:846-56 pubmed..These data show for the first time the expression of functional P2X7 receptors in a subpopulation of osteoblasts, activation of which can result in ATP-mediated apoptosis. ..
- Gilbert C, Green C, Lowndes N. Budding yeast Rad9 is an ATP-dependent Rad53 activating machine. Mol Cell. 2001;8:129-36 pubmed..This complex then catalyzes activation of Rad53 by acting as a scaffold that brings Rad53 molecules into close proximity, facilitating Rad53 in trans autophosphorylation and subsequent release of activated Rad53. ..
- Mason H, Bourke S, Kemp P. Selective modulation of ligand-gated P2X purinoceptor channels by acute hypoxia is mediated by reactive oxygen species. Mol Pharmacol. 2004;66:1525-35 pubmed..Furthermore, our data show that acute hypoxia selectively modulates the P2X2 receptor and that the response of P2X2 receptor subunits to hypoxia is mediated through the mitochondrial production of ROS. ..
- Chaudhuri N, Paiva C, Donaldson K, Duffin R, Parker L, Sabroe I. Diesel exhaust particles override natural injury-limiting pathways in the lung. Am J Physiol Lung Cell Mol Physiol. 2010;299:L263-71 pubmed publisher..These data describe a model of inflammation where DEP amplifies responses to low concentrations of microbial agonists and alters the nature of the inflammatory milieu induced by TLR agonists. ..
- Gandelman O, Church V, Moore C, Kiddle G, Carne C, Parmar S, et al. Novel bioluminescent quantitative detection of nucleic acid amplification in real-time. PLoS ONE. 2010;5:e14155 pubmed publisher..Since monitoring the BART reaction requires only a simple light detector, the iNAAT-BART combination is ideal for molecular diagnostic assays in both laboratory and low resource settings. ..
- Proks P, Capener C, Jones P, Ashcroft F. Mutations within the P-loop of Kir6.2 modulate the intraburst kinetics of the ATP-sensitive potassium channel. J Gen Physiol. 2001;118:341-53 pubmed..This gate appears to be able to operate independently of that which regulates the long interburst closings. ..
- Steffen W, Sleep J. Repriming the actomyosin crossbridge cycle. Proc Natl Acad Sci U S A. 2004;101:12904-9 pubmed..There was no significant movement of the actin during this interaction, so repriming must occur while myosin is dissociated, as in the original Lymn-Taylor scheme [Lymn, R. W. & Taylor, E. W. (1971) Biochemistry 10, 4617-4624]. ..
- Gourine A, Llaudet E, Dale N, Spyer K. Release of ATP in the ventral medulla during hypoxia in rats: role in hypoxic ventilatory response. J Neurosci. 2005;25:1211-8 pubmed..These data illustrate a new functional role for ATP-mediated purinergic signaling in the medullary mechanisms controlling respiratory activity. ..
- Bell C, Bright N, Rutter G, Griffiths E. ATP regulation in adult rat cardiomyocytes: time-resolved decoding of rapid mitochondrial calcium spiking imaged with targeted photoproteins. J Biol Chem. 2006;281:28058-67 pubmed..These results suggest that mitochondrial Ca(2+) transients occur during the contractile cycle and are translated into a time-averaged increase in mitochondrial ATP production that keeps pace with increased cytosolic demand. ..
- Gaskell A, Crack J, Kelemen G, Hutchings M, Le Brun N. RsmA is an anti-sigma factor that modulates its activity through a [2Fe-2S] cluster cofactor. J Biol Chem. 2007;282:31812-20 pubmed..This represents a major extension of the functional diversity of iron-sulfur cluster proteins. ..
- Boyd P, Tu W, Shorrock H, Groen E, Carter R, Powis R, et al. Bioenergetic status modulates motor neuron vulnerability and pathogenesis in a zebrafish model of spinal muscular atrophy. PLoS Genet. 2017;13:e1006744 pubmed publisher..We conclude that global bioenergetics pathways can be therapeutically manipulated to ameliorate SMA motor neuron phenotypes in vivo. ..
- Durand A, Merrick M. In vitro analysis of the Escherichia coli AmtB-GlnK complex reveals a stoichiometric interaction and sensitivity to ATP and 2-oxoglutarate. J Biol Chem. 2006;281:29558-67 pubmed..Our in vitro data suggest that in vivo association and dissociation of the complex might not only be dependent on the uridylylation status of GlnK but may also be influenced by intracellular pools of ATP and 2-oxoglutarate. ..
- Cao L, Young M, Broomhead H, Fountain S, North R. Thr339-to-serine substitution in rat P2X2 receptor second transmembrane domain causes constitutive opening and indicates a gating role for Lys308. J Neurosci. 2007;27:12916-23 pubmed..The correction of this gating defect, in the absence of any agonist, by the second mutation K308A shows that Lys308 is also involved in channel gating. A similar interpretation can account for the results with suramin and TNP-ATP. ..
- Parsons C, Tsaneva I, Lloyd R, West S. Interaction of Escherichia coli RuvA and RuvB proteins with synthetic Holliday junctions. Proc Natl Acad Sci U S A. 1992;89:5452-6 pubmed..We suggest that the RuvA and RuvB proteins play a specific role in the branch migration of Holliday junctions during postreplication repair of DNA damage in E. coli. ..
- O Regan P, Wilson C, Townsend S, Thacker J. XRCC2 is a nuclear RAD51-like protein required for damage-dependent RAD51 focus formation without the need for ATP binding. J Biol Chem. 2001;276:22148-53 pubmed..We conclude that XRCC2 is important in the early stages of homologous recombination in mammalian cells to facilitate RAD51-dependent recombination repair but that it does not make use of ATP binding to promote this function. ..
- Berovic N, Pratontep S, Bryant A, Montouris A, Green R. The kinetics of radiation damage to the protein luciferase and recovery of enzyme activity after irradiation. Radiat Res. 2002;157:122-7 pubmed..A few minutes after the pulse of protons, most of the enzyme activity has recovered. We attribute the fast recovery to the removal of charged ions, while the slow recovery involves refolding of denatured protein. ..
- Hoebertz A, Mahendran S, Burnstock G, Arnett T. ATP and UTP at low concentrations strongly inhibit bone formation by osteoblasts: a novel role for the P2Y2 receptor in bone remodeling. J Cell Biochem. 2002;86:413-9 pubmed..These findings suggest that extracellular nucleotides could function locally as important negative modulators of bone metabolism, perhaps contributing to bone loss in a number of pathological states. ..
- Sheehan B, Bosse J, Beddek A, Rycroft A, Kroll J, Langford P. Identification of Actinobacillus pleuropneumoniae genes important for survival during infection in its natural host. Infect Immun. 2003;71:3960-70 pubmed..The results highlight the importance of genes involved in energy metabolism, nutrient uptake and stress responses for the survival of A. pleuropneumoniae in its natural host: the pig...
- Tsuboi T, Da Silva Xavier G, Leclerc I, Rutter G. 5'-AMP-activated protein kinase controls insulin-containing secretory vesicle dynamics. J Biol Chem. 2003;278:52042-51 pubmed
- Whyteside G, Gibson L, Scott M, Finbow M. Assembly of the yeast vacuolar H+-ATPase and ATP hydrolysis occurs in the absence of subunit c''. FEBS Lett. 2005;579:2981-5 pubmed..However, we report that subunit c'' is not essential for assembly but is for functional coupling of the enzyme. ..
- Pellatt L, Rice S, Mason H. Phosphorylation and activation of AMP-activated protein kinase (AMPK) by metformin in the human ovary requires insulin. Endocrinology. 2011;152:1112-8 pubmed publisher..Although previous data suggest that metformin may act via an insulin-independent pathway, our results therefore imply that insulin may be required to initiate an effect. ..
- Rosenberg M, O Ryan L, Hughes G, Zhao Z, Aleksandrov L, Riordan J, et al. The cystic fibrosis transmembrane conductance regulator (CFTR): three-dimensional structure and localization of a channel gate. J Biol Chem. 2011;286:42647-54 pubmed publisher..Apart from the N and C termini, this indicated that in CFTR, the cytoplasmic end of transmembrane helix 5/11 and its associated loop could be partly disordered (or alternatively located). ..
- Rajebhosale M, Greenwood S, Vidugiriene J, Jeromin A, Hilfiker S. Phosphatidylinositol 4-OH kinase is a downstream target of neuronal calcium sensor-1 in enhancing exocytosis in neuroendocrine cells. J Biol Chem. 2003;278:6075-84 pubmed..Taken together, the present data indicate that NCS-1 modulates the activity of PI4K, leading to increased levels of phosphoinositides and concomitant enhancement of exocytosis. ..
- Clayton A, Al Taei S, Webber J, Mason M, Tabi Z. Cancer exosomes express CD39 and CD73, which suppress T cells through adenosine production. J Immunol. 2011;187:676-83 pubmed publisher..In summary, the data highlight exosome enzymic activity in the production of extracellular adenosine, and this may play a contributory role in negative modulation of T cells in the tumor environment. ..
- Butler P, Jones D. Physiology of diving of birds and mammals. Physiol Rev. 1997;77:837-99 pubmed..Efferent cardiac control is largely parasympathetic. Reflex cardiorespiratory responses are modulated by conditioning and habituation, but reflexes predominate during extended dives and during recovery, when gas exchange is maximized. ..
- Bordes P, Wigneshweraraj S, Schumacher J, Zhang X, Chaney M, Buck M. The ATP hydrolyzing transcription activator phage shock protein F of Escherichia coli: identifying a surface that binds sigma 54. Proc Natl Acad Sci U S A. 2003;100:2278-83 pubmed
- Morgan N, Pasha S, Johnson C, Ainsworth J, Eady R, Dawood B, et al. A germline mutation in BLOC1S3/reduced pigmentation causes a novel variant of Hermansky-Pudlak syndrome (HPS8). Am J Hum Genet. 2006;78:160-6 pubmed..These findings define a novel form of human HPS (HPS8) and extend genotype-phenotype correlations in HPS. ..
- Rissland O, Mikulasova A, Norbury C. Efficient RNA polyuridylation by noncanonical poly(A) polymerases. Mol Cell Biol. 2007;27:3612-24 pubmed..Finally, Hs2 (ZCCHC6), a human ortholog of Cid1, shows similar activity. These data suggest that uridylation of mRNA forms the basis of an evolutionarily conserved mechanism of gene regulation. ..
- Andersen D, Leevers S. The essential Drosophila ATP-binding cassette domain protein, pixie, binds the 40 S ribosome in an ATP-dependent manner and is required for translation initiation. J Biol Chem. 2007;282:14752-60 pubmed..Thus, the function of this soluble ATP-binding cassette domain protein family in translation initiation has been conserved from yeast through to higher eukaryotes...
- Garcia M, Knight M. Cyclic loading opens hemichannels to release ATP as part of a chondrocyte mechanotransduction pathway. J Orthop Res. 2010;28:510-5 pubmed publisher..In conclusion, mechanical loading triggers release of ATP via hemichannels. Hence, this study provides the first evidence of hemichannel involvement in chondrocyte mechanobiology. ..
- Holloway C, Cochlin L, Emmanuel Y, Murray A, Codreanu I, Edwards L, et al. A high-fat diet impairs cardiac high-energy phosphate metabolism and cognitive function in healthy human subjects. Am J Clin Nutr. 2011;93:748-55 pubmed publisher..01) after the high-fat, low-carbohydrate diet. Raising plasma free fatty acids decreased myocardial PCr/ATP and reduced cognition, which suggests that a high-fat diet is detrimental to heart and brain in healthy subjects. ..
- Swietach P, Youm J, Saegusa N, Leem C, Spitzer K, Vaughan Jones R. Coupled Ca2+/H+ transport by cytoplasmic buffers regulates local Ca2+ and H+ ion signaling. Proc Natl Acad Sci U S A. 2013;110:E2064-73 pubmed publisher..Given the abundance of cellular HDPs and ATP, spatial Ca(2+)/H(+) coupling is likely to be of general importance in cell signaling. ..
- Venet F, Demaret J, Blaise B, Rouget C, Girardot T, Idealisoa E, et al. IL-7 Restores T Lymphocyte Immunometabolic Failure in Septic Shock Patients through mTOR Activation. J Immunol. 2017;199:1606-1615 pubmed publisher..Our results support the rationale for targeting metabolism in sepsis with recombinant human IL-7 as a treatment option. ..
- Gibson L, Willows R, Kannangara C, Von Wettstein D, Hunter C. Magnesium-protoporphyrin chelatase of Rhodobacter sphaeroides: reconstitution of activity by combining the products of the bchH, -I, and -D genes expressed in Escherichia coli. Proc Natl Acad Sci U S A. 1995;92:1941-4 pubmed..The bchH, -I, and -D gene products are therefore assigned to the Mg chelatase step in bacteriochlorophyll biosynthesis. The mechanism of the Mg chelation reaction and the implications for chlorophyll biosynthesis in plants are discussed...
- Lang H, Cogdell R, Gardiner A, Hunter C. Early steps in carotenoid biosynthesis: sequences and transcriptional analysis of the crtI and crtB genes of Rhodobacter sphaeroides and overexpression and reactivation of crtI in Escherichia coli and R. sphaeroides. J Bacteriol. 1994;176:3859-69 pubmed..In this report, we have located the transcription initiation point and have shown that R. sphaeroides possesses an oxygen-regulated CrtI-type phytoene desaturase gene that forms a transcriptional operon with crtB...
- King B, Townsend Nicholson A, Wildman S, Thomas T, Spyer K, Burnstock G. Coexpression of rat P2X2 and P2X6 subunits in Xenopus oocytes. J Neurosci. 2000;20:4871-7 pubmed..Although homomeric P2X(6) receptors function poorly, the P2X(6) subunit can contribute to functional heteromeric P2X channels and may influence the phenotype of native P2X receptors in those cells in which it is expressed. ..
- Seki T, Diffley J. Stepwise assembly of initiation proteins at budding yeast replication origins in vitro. Proc Natl Acad Sci U S A. 2000;97:14115-20 pubmed..Extracts from cells arrested in mitosis also can support the binding of ORC but are unable to load either Cdc6p or MCM2-7 proteins. This system should be useful for studying the mechanism and regulation of pre-RC assembly. ..
- Harty C, Strahl S, Römisch K. O-mannosylation protects mutant alpha-factor precursor from endoplasmic reticulum-associated degradation. Mol Biol Cell. 2001;12:1093-101 pubmed..We conclude that there is a limited window of time during which misfolded proteins can be removed from the ER before they acquire inappropriate modifications that can interfere with disposal through the Sec61 channel. ..
- Krzywda S, Brzozowski A, Verma C, Karata K, Ogura T, Wilkinson A. The crystal structure of the AAA domain of the ATP-dependent protease FtsH of Escherichia coli at 1.5 A resolution. Structure. 2002;10:1073-83 pubmed..A sulfate anion in the ATP binding pocket mimics the beta-phosphate group of an adenine nucleotide. A hexamer form of FtsH has been modeled, providing insights into possible modes of nucleotide binding and intersubunit catalysis. ..
- Su Y, Zhou A, Al Lamki R, Karet F. The a-subunit of the V-type H+-ATPase interacts with phosphofructokinase-1 in humans. J Biol Chem. 2003;278:20013-8 pubmed..This interaction between the a-subunit and PFK-1 also provides new evidence that the C terminus of this subunit lies cytoplasmically in vivo. ..
- Tsuboi T, Lippiat J, Ashcroft F, Rutter G. ATP-dependent interaction of the cytosolic domains of the inwardly rectifying K+ channel Kir6.2 revealed by fluorescence resonance energy transfer. Proc Natl Acad Sci U S A. 2004;101:76-81 pubmed..probably involves both intrasubunit and intersubunit interactions, (iii). reflects ligand binding not channel gating, and (iv). occurs in intact cells when subplasmalemmal [ATP] changes in the millimolar range. ..
- Tregear R, Reedy M, Goldman Y, Taylor K, Winkler H, Franzini Armstrong C, et al. Cross-bridge number, position, and angle in target zones of cryofixed isometrically active insect flight muscle. Biophys J. 2004;86:3009-19 pubmed..Moreover, in the absence of filament sliding, cross-bridges cannot change tilt angle while attached nor reach other target zones while detached, so may cycle repeatedly on and off the same actin target monomer. ..
- Martinez Senac M, Webb M. Mechanism of translocation and kinetics of DNA unwinding by the helicase RecG. Biochemistry. 2005;44:16967-76 pubmed..The data support the idea that interactions of the duplex template arm with the protein are the major sites of binding and production of translocation...
- Abramov A, Fraley C, Diao C, Winkfein R, Colicos M, Duchen M, et al. Targeted polyphosphatase expression alters mitochondrial metabolism and inhibits calcium-dependent cell death. Proc Natl Acad Sci U S A. 2007;104:18091-6 pubmed..These results demonstrate a crucial role played by polyP in mitochondrial function of mammalian cells. ..
- Lall H, Coughlan K, Sumbayev V. HIF-1alpha protein is an essential factor for protection of myeloid cells against LPS-induced depletion of ATP and apoptosis that supports Toll-like receptor 4-mediated production of IL-6. Mol Immunol. 2008;45:3045-9 pubmed publisher..On the other hand HIF-1alpha protein is selectively required for down-regulation of ASK1 activated during LPS-induced TLR4 downstream signalling. ..
- Kunzelmann S, Webb M. A biosensor for fluorescent determination of ADP with high time resolution. J Biol Chem. 2009;284:33130-8 pubmed publisher..The broad applicability of N-[2-(1-maleimidyl)ethyl]-7-diethylaminocoumarin-3-carboxamide (MDCC)-ParM as a sensitive probe for ADP is demonstrated in real-time kinetic assays on two different ATPases and a protein kinase. ..
- Górna M, Pietras Z, Tsai Y, Callaghan A, Hernandez H, Robinson C, et al. The regulatory protein RraA modulates RNA-binding and helicase activities of the E. coli RNA degradosome. RNA. 2010;16:553-62 pubmed publisher..The multidentate interactions of RraA impede the RNA-binding and ribonuclease activities of the degradosome and may result in complex modulation and rerouting of degradosome activity. ..
- Hall C, Klein Flügge M, Howarth C, Attwell D. Oxidative phosphorylation, not glycolysis, powers presynaptic and postsynaptic mechanisms underlying brain information processing. J Neurosci. 2012;32:8940-51 pubmed publisher..Thus, the major mechanisms mediating brain information processing are all initially powered by oxidative phosphorylation, and an astrocyte-neuron lactate shuttle is not needed for this to occur. ..
- Schroeder M, Ali M, Hulikova A, Supuran C, Clarke K, Vaughan Jones R, et al. Extramitochondrial domain rich in carbonic anhydrase activity improves myocardial energetics. Proc Natl Acad Sci U S A. 2013;110:E958-67 pubmed publisher..Aberrant distribution of CA activity therefore may reduce the heart's energetic efficiency. ..
- Shintani Y, Kapoor A, Kaneko M, Smolenski R, D Acquisto F, Coppen S, et al. TLR9 mediates cellular protection by modulating energy metabolism in cardiomyocytes and neurons. Proc Natl Acad Sci U S A. 2013;110:5109-14 pubmed publisher..These data propose an intriguing model that the same ligand-receptor can concomitantly increase the stress tolerance in cardiomyocytes and neurons, whereas immune cells induce inflammation upon tissue injury. ..