Experts and Doctors on swine diseases in Wuhan, Hubei, China


Locale: Wuhan, Hubei, China
Topic: swine diseases

Top Publications

  1. Yang W, Ying F, Yingyu L, Pin C, Wentao L, Shuqing L, et al. Development and evaluation of loop-mediated isothermal amplification for rapid detection of Haemophilus parasuis. FEMS Microbiol Lett. 2010;313:54-60 pubmed publisher
    ..parasuis from samples obtained from experimentally infected pigs. We concluded that LAMP is a highly sensitive and reliable method for detection of H. parasuis infection...
  2. Zhang L, Li K, Huang S, Liu D, Rehman M, Lan Y, et al. Seroprevalence and risk factors associated with hepatitis E virus infections among people and pigs in Tibet, China. Acta Trop. 2017;172:102-106 pubmed publisher
    ..Our findings confirmed the presence of autochthonous HEV among people and pigs in Tibet, China. Therefore, effective measures should be taken to prevent and control HEV infection in Tibet, China. ..
  3. Wu Y, Chen D, He H, Chen D, Chen L, Chen H, et al. Pseudorabies virus infected porcine epithelial cell line generates a diverse set of host microRNAs and a special cluster of viral microRNAs. PLoS ONE. 2012;7:e30988 pubmed publisher
    ..Collectively, these data suggest that PRV infected epithelial cell line generates a diverse set of host miRNAs and a special cluster of viral miRNAs, which might facilitate PRV replication in cells...
  4. Chen H, Lunney J, Cheng L, Li X, Cao J, Zhu M, et al. Porcine S100A8 and S100A9: molecular characterizations and crucial functions in response to Haemophilus parasuis infection. Dev Comp Immunol. 2011;35:490-500 pubmed publisher
    ..A pS100A8/pS100A9-CASP3-SLC1A2 pathway regulating lipid metabolism was found. Both of their pro- and anti-inflammatory functions in response to HPS infection are highlighted...
  5. Wang D, Fang L, Zhao F, Luo R, Chen H, Xiao S. Molecular cloning, expression and antiviral activity of porcine interleukin-29 (poIL-29). Dev Comp Immunol. 2011;35:378-84 pubmed publisher
  6. Zhang A, Mu X, Chen B, Liu C, Han L, Chen H, et al. Identification and characterization of IgA1 protease from Streptococcus suis. Vet Microbiol. 2010;140:171-5 pubmed publisher
    ..The gene was found to exist in most pathogenic strains but scarcely in non-invasive isolates. These findings suggested that the IgA1 protease might play an important role in the pathogenesis of S. suis...
  7. Tu J, Zhou H, Jiang T, Li C, Zhang A, Guo X, et al. Isolation and molecular characterization of equine H3N8 influenza viruses from pigs in China. Arch Virol. 2009;154:887-90 pubmed publisher
    ..This expansion of host range of equine H3N8 influenza viruses with mutations in the HA protein might raise the possibility of transmission of these viruses to humans. ..
  8. Qing L, Lv J, Li H, Tan Y, Hao H, Chen Z, et al. The recombinant nonstructural polyprotein NS1 of porcine parvovirus (PPV) as diagnostic antigen in ELISA to differentiate infected from vaccinated pigs. Vet Res Commun. 2006;30:175-90 pubmed
    ..In conclusion, the PPV-NS1 ELISA can differentiate PPV-infected versus inactivated PPV-vaccinated pigs and could be applied in disease diagnosis and surveillance...
  9. Xinglin J, Huanchun C, Xiang W, Changming Q. Quantitative and qualitative study of enzyme-linked immunosorbent assay to detect IgG against Japanese encephalitis virus in swine sera. Vet Res Commun. 2005;29:159-69 pubmed
    ..05). This ELISA test can detect 46 swine serum samples qualitatively and the titre of eight swine serum samples through endpoint dilution quantitatively within one 96-well plate...

More Information


  1. Li W, Li H, Liu Y, Pan Y, Deng F, Song Y, et al. New variants of porcine epidemic diarrhea virus, China, 2011. Emerg Infect Dis. 2012;18:1350-3 pubmed publisher
    ..Three new PEDV variants were identified...
  2. Liu W, Yang M, Xu Z, Zheng H, Liang W, Zhou R, et al. Complete genome sequence of Pasteurella multocida HN06, a toxigenic strain of serogroup D. J Bacteriol. 2012;194:3292-3 pubmed publisher
    ..Here, we report the complete genome sequence of P. multocida HN06, a toxigenic serogroup D strain isolated from a diseased pig in China...
  3. Zhao M, Liu X, Li X, Chen H, Jin H, Zhou R, et al. Systems infection biology: a compartmentalized immune network of pig spleen challenged with Haemophilus parasuis. BMC Genomics. 2013;14:46 pubmed publisher
    ..Our findings complement and extend the frontiers of knowledge of host infection biology for H. parasuis and also provide a new clue for systems infection biology of Gram-negative bacilli in mammals. ..
  4. Xu Z, Chen H, Zhou R. Genome-wide evidence for positive selection and recombination in Actinobacillus pleuropneumoniae. BMC Evol Biol. 2011;11:203 pubmed publisher
    ..The genome-wide profile of positively selected genes and/or amino acid residues will provide valuable targets for further research into the mechanisms of immune evasion and host-pathogen interactions for this serious swine pathogen. ..
  5. Liu X, Chen H, Tong Q, Li X, Zhu M, Wu Z, et al. Molecular characterization of caveolin-1 in pigs infected with Haemophilus parasuis. J Immunol. 2011;186:3031-46 pubmed publisher
    ..In addition, our results suggest that Cav1 may be implicated in the pathogenesis of Glasser's disease, which is caused by H. parasuis...
  6. Cai X, Chen H, Blackall P, Yin Z, Wang L, Liu Z, et al. Serological characterization of Haemophilus parasuis isolates from China. Vet Microbiol. 2005;111:231-6 pubmed
    ..Streptococcus suis (30.6%; 56), Escherichia coli (21.9%; 40), Bordetella bronchiseptica (21.3%; 39) and Pasteurella multocida (14.2%; 26) were the bacterial agents frequently co-isolated with H. parasuis in China...
  7. Li J, Tan C, Zhou Y, Fu S, Hu L, Hu J, et al. The two-component regulatory system CiaRH contributes to the virulence of Streptococcus suis 2. Vet Microbiol. 2011;148:99-104 pubmed publisher
    ..These results suggest that the CiaRH is required for S. suis 2 virulence...
  8. Xu Z, Chen X, Li L, Li T, Wang S, Chen H, et al. Comparative genomic characterization of Actinobacillus pleuropneumoniae. J Bacteriol. 2010;192:5625-36 pubmed publisher
    ..pleuropneumoniae and provide broader insight into both pathogenesis research and clinical/epidemiological application against the severe disease caused by this swine pathogen. ..
  9. Qian P, Li X, Jin M, Peng G, Chen H. An approach to a FMD vaccine based on genetic engineered attenuated pseudorabies virus: one experiment using VP1 gene alone generates an antibody responds on FMD and pseudorabies in swine. Vaccine. 2004;22:2129-36 pubmed
  10. Zhou M, Guo Y, Zhao J, Hu Q, Hu Y, Zhang A, et al. Identification and characterization of novel immunogenic outer membrane proteins of Haemophilus parasuis serovar 5. Vaccine. 2009;27:5271-7 pubmed publisher
    ..parasuis. The results suggest these 4 proteins (PalA, Omp2, D15 and HPS_06257) have strong potential to be vaccine candidates...
  11. Huang H, Zhou R, Fan H, Dan H, Chen M, Yan L, et al. Generation of monoclonal antibodies and epitope mapping of ApxIVA of Actinobacillus pleuropneumoniae. Mol Immunol. 2006;43:2130-4 pubmed
    ..In conclusion, we have succeeded in producing eight monoclonal antibodies, which react with five different epitopes of ApxIV. ..
  12. Fu S, Yuan F, Zhang M, Tan C, Chen H, Bei W. Cloning, expression and characterization of a cell wall surface protein, 6-phosphogluconate dehydrogenase, of Haemophilus parasuis. Res Vet Sci. 2012;93:57-62 pubmed publisher
    ..Identification and characterization of the role of H. parasuis 6PGD in adhesion and immunogenicity will allow us to use this protein to develop new antimicrobial therapies and/or vaccines...
  13. Chen H, Li C, Fang M, Zhu M, Li X, Zhou R, et al. Understanding Haemophilus parasuis infection in porcine spleen through a transcriptomics approach. BMC Genomics. 2009;10:64 pubmed publisher
    ..Our data provide new clues to the nature of the immune response in mammals, and we have identified candidate genes that are related to resistance to HPS. ..
  14. Wang D, Fang L, Li K, Zhong H, Fan J, Ouyang C, et al. Foot-and-mouth disease virus 3C protease cleaves NEMO to impair innate immune signaling. J Virol. 2012;86:9311-22 pubmed publisher
    ..Collectively, our data identify NEMO as a substrate for FMDV 3C(pro) and reveal a novel mechanism evolved by a picornavirus to counteract innate immune signaling...
  15. Liu C, Chen Z, Tan C, Liu W, Xu Z, Zhou R, et al. Immunogenic characterization of outer membrane porins OmpC and OmpF of porcine extraintestinal pathogenic Escherichia coli. FEMS Microbiol Lett. 2012;337:104-11 pubmed publisher
    ..Furthermore, the selection analysis indicated that gene ompC may be subject to strong immune pressure. Our results demonstrated that OmpC is a promising vaccine target against ExPEC infections in swine...
  16. Hu Q, Liu P, Yu Z, Zhao G, Li J, Teng L, et al. Identification of a cell wall-associated subtilisin-like serine protease involved in the pathogenesis of Streptococcus suis serotype 2. Microb Pathog. 2010;48:103-9 pubmed publisher
    ..These data indicated that the surface-associated protein SspA is a conserved virulence factor of S. suis and is involved in the pathogenesis of S. suis. ..
  17. Wang X, Wu C, Song X, Chen H, Liu Z. Comparison of pseudorabies virus China reference strain with emerging variants reveals independent virus evolution within specific geographic regions. Virology. 2017;506:92-98 pubmed publisher
  18. Yong T, Huan chun C, Shao bo X, Ya li Q, Qi gai H, Yu qi R. Development of a latex agglutination test using the major epitope domain of glycoprotein E of pseudorabies virus expressed in E. coli to differentiate between immune responses in pigs naturally infected or vaccinated with pseudorabies virus. Vet Res Commun. 2005;29:487-97 pubmed
    ..The data indicate that the gE-LAT is of similar sensitivity to gE-ELISA. These results indicate that gE-LAT using recombinant gE might be very useful as a routine screening method for the differential diagnosis of PRV infection. ..
  19. Lu J, Zhao J, Fang L, He Q, Cao S, Chen H. A slide latex agglutination test for the rapid detection of antibodies in serum against porcine parvovirus. J Vet Med B Infect Dis Vet Public Health. 2006;53:59-61 pubmed
    ..A good agreement between LAT and haemagglutination inhibit assay was observed. Because of convenience and speed of performance, this method would be used widely in clinic examination...
  20. Wang X, Xu X, Wu Y, Li L, Cao R, Cai X, et al. Polysaccharide biosynthesis protein CapD is a novel pathogenicity-associated determinant of Haemophilus parasuis involved in serum-resistance ability. Vet Microbiol. 2013;164:184-9 pubmed publisher
    ..These data present the evidence that the capD gene is a novel pathogenicity-associated determinant and involved in serum-resistance ability of H. parasuis. ..
  21. Jin H, Zhou R, Kang M, Luo R, Cai X, Chen H. Biofilm formation by field isolates and reference strains of Haemophilus parasuis. Vet Microbiol. 2006;118:117-23 pubmed
    ..parasuis could form biofilms in vitro, and the biofilm formation phenotype is associated with the recovery site of the strains and is maintained when bacteria are passaged in vitro and in the upper respiratory tract...
  22. Fu S, Ou J, Zhang M, Xu J, Liu H, Liu J, et al. The live attenuated Actinobacillus pleuropneumoniae triple-deletion mutant ?apxIC ?apxIIC ?apxIV-ORF1 strain, SLW05, Immunizes pigs against lethal challenge with Haemophilus parasuis. Clin Vaccine Immunol. 2013;20:134-9 pubmed publisher
    ..This is the first report that a live attenuated A. pleuropneumoniae vaccine with SLW05 can protect against lethal H. parasuis infection, which provides a novel approach for developing an attenuated H. parasuis vaccine...
  23. Wang S, Liu W, Yang L, Sargent C, Liu H, Wang C, et al. Effects of FUT1 gene mutation on resistance to infectious disease. Mol Biol Rep. 2012;39:2805-10 pubmed publisher
    ..It can be concluded that the mutation of FUT1 gene might play a role in pig infection by multi-pathogens, and that AA may be a favourable genotype for increasing the resistance to disease...
  24. Shi Y, Hu Z, Xiong Z, Zhou Y, Jin X, Gu C, et al. Analysis of molecular variation of porcine reproductive and respiratory syndrome virus in Central China from 2006 to 2012. Arch Virol. 2013;158:717-21 pubmed publisher
    ..Furthermore, we found that the number of potential N-glycosylation sites had apparently increased since 2006. These findings increase our knowledge of PRRSV epidemiology in Central China...
  25. Ju C, Fan H, Tan Y, Liu Z, Xi X, Cao S, et al. Immunogenicity of a recombinant pseudorabies virus expressing ORF1-ORF2 fusion protein of porcine circovirus type 2. Vet Microbiol. 2005;109:179-90 pubmed
    ..This is a first step toward the development of a potential candidate divalent vaccine against PRV and PCV2 infections...
  26. Du F, Zhang Q, Yu Q, Hu M, Zhou Y, Zhao J. Soil contamination of Toxoplasma gondii oocysts in pig farms in central China. Vet Parasitol. 2012;187:53-6 pubmed publisher
    ..gondii oocysts in Hubei Province. The soil contamination status of pig farms was significantly influenced by cat density and may be an indicator of T. gondii infection in pigs...
  27. Yang W, Pin C, Haibing G, Yang C, Hui L, Qigai H. Loop-mediated isothermal amplification targeting the apxIVA gene for detection of Actinobacillus pleuropneumoniae. FEMS Microbiol Lett. 2009;300:83-9 pubmed publisher
    ..0001); we concluded that LAMP was a highly sensitive and reliable method for detection of A. pleuropneumoniae infection...
  28. Feng S, Chen X, Xia T, Gan L, Qiu H, Dai M, et al. Cloning, chromosome mapping and expression characteristics of porcine ANGPTL3 and -4. Cytogenet Genome Res. 2006;114:44-9 pubmed
    ..This is the first report of molecular cloning and characterization of ANGPTL3 and -4 in pigs, which will be helpful for a better understanding of the role of ANGPTLs in lipid metabolism. ..