Experts and Doctors on polymerase chain reaction in Lanzhou, Gansu Sheng, China


Locale: Lanzhou, Gansu Sheng, China
Topic: polymerase chain reaction

Top Publications

  1. Yang Y, Yang A, Yang Z, Wang Z, Xia X. Involvement of tumor suppressor in lung cancer 1 gene expression in cervical carcinogenesis. Int J Gynecol Cancer. 2006;16:1868-72 pubmed
    ..05). The results show that loss of TSLC1 gene expression is an early event in cervical carcinogenesis and often accompanies invasive cervical cancers. ..
  2. Ni Y, Yang Y, Bao J, He K, Li H. Inter- and intraspecific genomic variability of the 16S-23S intergenic spacer regions (ISR) in representatives of Acidithiobacillus thiooxidans and Acidithiobacillus ferrooxidans. FEMS Microbiol Lett. 2007;270:58-66 pubmed
    ..ISR may be a potential target for the development of in situ hybridization probe aimed at accurately detecting acidithiobacilli in the various acidic environments...
  3. Lu J, Li Y, Jin Y, Li L. [Association of the vitamin D receptor gene start codon polymorphism with delayed rickets]. Zhonghua Er Ke Za Zhi. 2007;45:46-9 pubmed
    ..The present study aimed to explore the hereditary susceptibility of children to development of delayed rickets through studying the association of the vitamin D receptor gene start codon (VDRSC) polymorphism with delayed rickets...
  4. Yang G, Zhou H, Forrest R, Fang Q, Luo Y, Hickford J. Halplotypes of the ovine ADRB3 gene (ADRB3) and their association with post-weaning growth in New Zealand Suffolk sheep. Mol Biol Rep. 2013;40:4805-10 pubmed publisher
    ..The effects of the ovine ADRB3 haplotypes on weaning-weight and post-weaning growth confirm that they could be used as a candidate gene-marker for improving sheep growth. ..
  5. Ni Y, He K, Bao J, Yang Y, Wan D, Li H. Genomic and phenotypic heterogeneity of Acidithiobacillus spp. strains isolated from diverse habitats in China. FEMS Microbiol Ecol. 2008;64:248-59 pubmed publisher
    ..The results showed that the Chinese Acidithiobacillus spp. isolates exhibited a high degree of genomic and phenotypic heterogeneity...
  6. Wang D, Wang Z, Tian J, He X, Chowdhury W, Zhang X, et al. Prostate stem cell antigen enhancer and uroplakin II promoter based bladder cancer targeted tissue-specific vector. Urol Oncol. 2010;28:164-9 pubmed publisher
    ..PSCAE is active in both AR positive and AR negative bladder cancer cells. The results suggest that combination of PSCAE with UPII promoter is feasible in constructing bladder cancer-specific vectors. ..
  7. Yan W, Zhou H, Luo Y, Hu J, Hickford J. Allelic variation in ovine fatty acid-binding protein (FABP4) gene. Mol Biol Rep. 2012;39:10621-5 pubmed publisher
    ..These results indicate that ovine FABP4 is polymorphic and suggest further analysis is required to see if the variation detected affects fat deposition in sheep...
  8. Yan X, Chu Y, Wu G, Zhao Z, Li J, Zhu H, et al. An outbreak of sheep pox associated with goat poxvirus in Gansu province of China. Vet Microbiol. 2012;156:425-8 pubmed publisher
    ..The results characterized the isolated viruses as GTPV. It is the first report of an outbreak of sheep pox associated with GTPV in China...
  9. Yang G, Zhou H, Hu J, Luo Y, Hickford J. Extensive diversity of the ADRB3 gene in Chinese sheep identified by PCR-SSCP. Biochem Genet. 2009;47:498-502 pubmed publisher
    ..0%). These results suggest that Chinese sheep have more diversity in ADRB3 than New Zealand sheep and therefore have possibly been selected either naturally or via breeding for different traits. ..

More Information


  1. Chu Y, Yan X, Gao P, Zhao P, He Y, Liu J, et al. Molecular detection of a mixed infection of Goatpox virus, Orf virus, and Mycoplasma capricolum subsp. capripneumoniae in goats. J Vet Diagn Invest. 2011;23:786-9 pubmed publisher
    ..The PCR products were cloned, sequenced, and aligned with related reference sequences in GenBank for further identification of the pathogens. The present study reports a mixed infection with GTPV, ORFV, and MCCP in goats...
  2. Huang S, Zhao G, Fu B, Xu M, Wang C, Wu S, et al. Genomics and molecular genetics of Clonorchis sinensis: current status and perspectives. Parasitol Int. 2012;61:71-6 pubmed publisher
    ..The advances in genomics and molecular genetics of C. sinensis shed new sight on our understanding of population structure of C. sinensis as well as the prevention and control of clonorchiasis...
  3. Wu W, Li C, Chen R, Cao X, Li A, Wang Y, et al. [A case-control study on JWA promoter -76G-->C polymorphism and the susceptibility of bladder cancer]. Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2005;22:648-52 pubmed
    ..The JWA -76G-->C variant genotype may play an important role in transcription regulation of JWA gene and in the susceptibility to bladder cancer. ..
  4. Hu J, Zhou H, Smyth A, Luo Y, Hickford J. Polymorphism of the bovine ADRB3 gene. Mol Biol Rep. 2010;37:3389-92 pubmed publisher
    ..83%), whereas alleles C, D, E and F were rare (0.07, 1.09, 0.41, and 0.34%, respectively). The variation identified here might have an impact on both the function and level of expression of bovine ADRB3. ..
  5. Lin R, Liu G, Zhang Y, D Amelio S, Zhou D, Yuan Z, et al. Contracaecum rudolphii B: gene content, arrangement and composition of its complete mitochondrial genome compared with Anisakis simplex s.l. Exp Parasitol. 2012;130:135-40 pubmed publisher
    ..rudolphii B, and should have implications for the molecular diagnosis, prevention and control of anisakidosis in humans and animals...
  6. Liu Z, Ma M, Wang Z, Wang J, Peng Y, Li Y, et al. Molecular survey and genetic identification of Anaplasma species in goats from central and southern China. Appl Environ Microbiol. 2012;78:464-70 pubmed publisher
    ..bovis in the infected monocytes of sheep. Phylogenetic study with msp4 sequences of A. ovis indicated that the A. ovis genotypes from sheep in the north differed from the genotypes of goats in the investigated sites. ..
  7. Zhao Y, Ju F, Zhao Y, Wang L, Sun Z, Liu M, et al. The expression of ?A- and ?B1-crystallin during normal development and regeneration, and proteomic analysis for the regenerating lens in Xenopus laevis. Mol Vis. 2011;17:768-78 pubmed
  8. Zhou H, Hu J, Luo Y, Hickford J. Variation in the ovine C-type lectin dectin-1 gene (CLEC7A). Dev Comp Immunol. 2010;34:246-9 pubmed publisher
    ..Of these SNPs, the two in exon 4 and two in exon 6 would result in amino acid substitutions. The variation detected here may have an impact on the recognition of beta-glucans and therefore affect the ovine immune response...
  9. Chen H, Zhang J, Ma Y, Ma L, Ding Y, Liu X, et al. Reverse transcription loop-mediated isothermal amplification for the rapid detection of infectious bronchitis virus in infected chicken tissues. Mol Cell Probes. 2010;24:104-6 pubmed publisher
    ..5% and 98.4%, respectively. These findings showed that the RT-LAMP assay has potential usefulness for rapid and sensitive diagnosis in outbreak of IBV. ..
  10. Hua S, Li S, Tan H, Zhao J. [Sequence analysis of 16S rDNA and genes of soluble methane monooxygenase from Methylomonas sp. GYJ3]. Wei Sheng Wu Xue Bao. 2007;47:150-5 pubmed
    ..The theoretical pI of hydroxylase was 6.28 and the theoretical MW of hydroxylase was 248874.41Da...
  11. Jian B, Liu B, Bi Y, Hou W, Wu C, Han T. Validation of internal control for gene expression study in soybean by quantitative real-time PCR. BMC Mol Biol. 2008;9:59 pubmed publisher
    ..To achieve accurate results, some conditions may require more than one HKG to be used for normalization. ..
  12. Wu G, Wang Z, Zhang H, Yang N, Du J, Lu X, et al. Electrochemical detection of beta-1,3-glucanase gene from transgenic capsicums using asymmetric PCR generated by a detecting probe and an anchoring probe. J Biotechnol. 2010;145:1-8 pubmed publisher
    ..The estimated DPV detection limits for three targets of oligonucleotide, the molecular cloning vector DNA and genome DNA were 2.6x10(-13), 6.0x10(-13) and 8.0x10(-13)moll(-1) respectively. ..
  13. Hua S, Li S. [Molecular analysis of soluble methane monooxygenase and 16S rDNA from a type II methanotroph]. Wei Sheng Wu Xue Bao. 2009;49:294-301 pubmed
    ..The soluble methane monooxygenase (sMMO) from Methylosinus trichosporium IMV 3011 catalyzes the conversion of methane to methanol...
  14. Yin H, Liu Z, Guan G, Liu A, Ma M, Ren Q, et al. Detection and differentiation of Theileria luwenshuni and T. uilenbergi infection in small ruminants by PCR. Transbound Emerg Dis. 2008;55:233-7 pubmed publisher
    ..This suggests that these PCR assays are useful tools for the detection of and differentiation between T. luwenshuni and T. uilenbergi...
  15. Wu G, Wang Z, Zhang H, Yang N, Du J, Lu X, et al. An electrochemical assay of beta-1,3-glucanase gene from transgenic capsicum using asymmetric PCR. Nucleosides Nucleotides Nucleic Acids. 2009;28:1051-67 pubmed publisher
    ..The detection limits of 2.6 x 10(-13), 7.8 x 10(-13), and 9.1 x 10(-13) moll(-1) for oligonucleotide, molecule clone vector DNA, and total transgenic capsicum genome DNA were estimated. ..
  16. Jiang H, Huang S, Zhou D, Zhang X, Su C, Deng S, et al. Genetic characterization of Toxoplasma gondii from pigs from different localities in China by PCR-RFLP. Parasit Vectors. 2013;6:227 pubmed publisher
    ..gondii isolates from pigs in Jiangxi, Sichuan, Guangdong Provinces and Chongqing Municipality in China using multilocous polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technology...
  17. Pan Y, Zeng Q, Zhu C, Hua X, Wang M, Pan K, et al. Frequency and characterization of porcine hokovirus (PHoV) in domestic pigs in eastern China. Arch Virol. 2012;157:1785-8 pubmed publisher
    ..The results provide evidence that there are differences between PHoV in China compared to early isolates. PHoV infections are more frequent in 0- to 6-week-old pigs than in older ones...
  18. Lin R, Liu G, Song H, Zhang Y, Li M, Zou F, et al. Sequence variability in three mitochondrial genes between the two pig nodule worms Oesophagostomum dentatum and O. quadrispinulatum. Mitochondrial DNA. 2012;23:182-6 pubmed publisher
    ..These findings demonstrated clearly the usefulness of the three mitochondrial sequences for studying systematics, population genetic structures, and the molecular ecology of Oesophagostomum spp...
  19. Tian Z, Liu G, Shen H, Xie J, Luo J, Tian M. First report on the occurrence of Rickettsia slovaca and Rickettsia raoultii in Dermacentor silvarum in China. Parasit Vectors. 2012;5:19 pubmed publisher
    ..Although new spotted fever group rickettsiae have been isolated in many parts of the world including China, Little is known about the epidemiology of Rickettsia pathogens in ticks from Xinjiang Autonomous Region of China...
  20. Li Y, Wang Z, Tian J, Tian B, Rodrigues R, Shang P, et al. Dendritic cell transfected with secondary lymphoid-tissue chemokine and/or interleukin-2 gene-enhanced cytotoxicity of T-lymphocyte in human bladder tumor cell S in vitro. Cancer Invest. 2009;27:909-17 pubmed publisher
    ..DC/SLC-IL-2 can promote DC proliferation, while TC-DC/SLC-IL-2 and TC-DC/SLC could strongly enhance significant cytotoxicity against bladder cancer cell that was induced by the coculture of DCs (transfected with SLC and IL-2) and TC. ..
  21. Song H, Mo X, Zhao G, Li J, Zou F, Liu W, et al. Electrophoretic detection of genetic variability among Schistosoma japonicum isolates by sequence-related amplified polymorphism. Electrophoresis. 2011;32:1364-70 pubmed publisher
    ..These results indicate the usefulness of the SRAP technique for revealing genetic variability among S. japonicum isolates from China, and the SRAP technique should be applicable to other living organisms. ..
  22. Tian Z, Liu G, Xie J, Yin H, Luo J, Zhang L, et al. Discrimination between Haemaphysalis longicornis and H. qinghaiensis based on the partial 16S rDNA and the second internal transcribed spacer (ITS-2). Exp Appl Acarol. 2011;54:165-72 pubmed publisher
  23. Zhao G, Li J, Song H, Li X, Chen F, Lin R, et al. A specific PCR assay for the identification and differentiation of Schistosoma japonicum geographical isolates in mainland China based on analysis of mitochondrial genome sequences. Infect Genet Evol. 2012;12:1027-36 pubmed publisher
    ..These findings indicate that the specific PCR assay would provide a useful tool for the epidemiological surveillance and for tracing the source of S. japonicum infection in humans and animals in China...
  24. Fu B, Liu M, Yao C, Li W, Li Y, Wang Y, et al. Species identification of Trichinella isolates from China. Vet Parasitol. 2009;159:214-7 pubmed publisher
    ..Sequence analysis of the 5S rDNA inter-gene spacer region from the ten Trichinella isolates confirmed the existence of only two Trichinella species and revealed the inner species genetic variation within T. spiralis and T. nativa. ..