Experts and Doctors on ephemeral fever in Lanzhou, Gansu Sheng, China
Locale: Lanzhou, Gansu Sheng, China
Topic: ephemeral fever
Zheng F, Lin G, Qiu C. [Expression, purification and antigenic characterization of the Epitope-G1 gene of bovine ephemeral fever virus in Escherichia coli]. Wei Sheng Wu Xue Bao. 2007;47:498-502 pubmed
..All the results above indicated that the target protein expressed had nicer biological activity and specificity, so the protein could be used as coating antigen to develop ELISA Kit for diagnosing BEF...
Zheng F, Lin G, Qiu C, Zhou J, Cao X, Gong X. Serological detection of bovine ephemeral fever virus using an indirect ELISA based on antigenic site G1 expressed in Pichia pastoris. Vet J. 2010;185:211-5 pubmed publisher
..The ELISA is an inexpensive and rapid serological detection method that would be suitable for screening for BEFV infection on a large scale...
Zheng F, Lin G, Zhou J, Wang G, Cao X, Gong X, et al
. A reverse-transcription, loop-mediated isothermal amplification assay for detection of bovine ephemeral fever virus in the blood of infected cattle. J Virol Methods. 2011;171:306-9 pubmed publisher
..The RT-LAMP method is useful for the diagnosis of BEFV infection in blood samples...
Zheng F, Chen Q, Li Z, Gong X, Wang J, Yin H. Experimental infection with bovine ephemeral fever virus and analysis of its antibody response cattle. Res Vet Sci. 2016;104:146-51 pubmed publisher
..However, after 3 or 6months, when nAbs were no longer apparent, re-infected cattle displayed typical symptoms of BEF. Our findings indicated that vaccination should be performed once the titer of nAb decreased to 5 or 6. ..