Experts and Doctors on escherichia coli proteins in Hefei, Anhui Sheng, China


Locale: Hefei, Anhui Sheng, China
Topic: escherichia coli proteins

Top Publications

  1. Wang W, Wang L, Wu J, Gong Q, Shi Y. Hfq-bridged ternary complex is important for translation activation of rpoS by DsrA. Nucleic Acids Res. 2013;41:5938-48 pubmed publisher
    ..This ternary complex possibly corresponds to a meta-stable transition state in Hfq-facilitated small RNA-mRNA annealing process. ..
  2. Fang P, Li X, Wang J, Xing L, Gao Y, Niu L, et al. Crystal structure of the protein L-isoaspartyl methyltransferase from Escherichia coli. Cell Biochem Biophys. 2010;58:163-7 pubmed publisher
    ..8 Å. The enzyme we investigated was able to remain bound to its product S-adenosylhomocysteine (SAH) during crystallization. Analysis indicates that the high affinity of EcPIMT for SAH might lead to the lower activity of the enzyme. ..
  3. Wang W, Wang L, Zou Y, Zhang J, Gong Q, Wu J, et al. Cooperation of Escherichia coli Hfq hexamers in DsrA binding. Genes Dev. 2011;25:2106-17 pubmed publisher
    ..Fluorescence resonance energy transfer conducted with full-length Hfq and DsrA also supports cooperation of Hfq hexamers upon DsrA binding. The implications of Hfq hexamer cooperation have been discussed. ..
  4. Li Y, Pan M, Li Y, Huang Y, Guo Q. Thiol-yne radical reaction mediated site-specific protein labeling via genetic incorporation of an alkynyl-L-lysine analogue. Org Biomol Chem. 2013;11:2624-9 pubmed publisher
    ..With these alkyne handles, site-specific dual labeling of proteins can be achieved via a bioorthogonal thiol-yne ligation reaction. ..
  5. Wei Y, Zhang H, Gao Z, Wang W, Shtykova E, Xu J, et al. Crystal and solution structures of methyltransferase RsmH provide basis for methylation of C1402 in 16S rRNA. J Struct Biol. 2012;179:29-40 pubmed publisher
    ..In general, crystal and solution structural analysis provides new information on the mechanism of the methylation of the fine-tuning ribosomal decoding center by the RsmH. ..
  6. Xue T, Zhao L, Sun H, Zhou X, Sun B. LsrR-binding site recognition and regulatory characteristics in Escherichia coli AI-2 quorum sensing. Cell Res. 2009;19:1258-68 pubmed publisher
    ..Furthermore, in agreement with the conclusion that phosphorylated AI-2 can relieve the repression of LsrR in previous studies, our data show that phospho-AI-2 renders LsrR unable to bind to its own promoter in vitro. ..
  7. Wu C, Cheng Y, Yin H, Song X, Li W, Zhou X, et al. Oxygen promotes biofilm formation of Shewanella putrefaciens CN32 through a diguanylate cyclase and an adhesin. Sci Rep. 2013;3:1945 pubmed publisher
    ..Regulation of DosD in aerobic biofilms is heavily dependent on an adhesin BpfA and the TISS. This study offers an insight into the molecular mechanism of oxygen-stimulated biofilm formation by S. putrefaciens CN32...
  8. Wu M, Tao Y, Liu X, Zang J. Structural basis for phosphorylated autoinducer-2 modulation of the oligomerization state of the global transcription regulator LsrR from Escherichia coli. J Biol Chem. 2013;288:15878-87 pubmed publisher
    ..Based on these observations, we propose that phosphorylated autoinducer-2 triggers the disassembly of the LsrR tetramer to activate the transcription of its target genes. ..
  9. Xing L, Zhu Y, Fang P, Wang J, Zeng F, Li X, et al. Crystallization and preliminary crystallographic studies of UbiG, an O-methyltransferase from Escherichia coli. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011;67:727-9 pubmed publisher
    ..2 Å, β = 105.3°. Both Matthews coefficient analysis and the self-rotation function suggested the presence of one molecule per asymmetric unit in the crystal, with a solvent content of 50.52% (V(M) = 2.48 Å(3) Da(-1)). ..

More Information


  1. Zhang M, Pan R, Yu Z, Wu L. [Assessment of the Escherichia coli Tat protein translocation system with fluorescent proteins]. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 2003;35:702-6 pubmed
  2. Fang P, Wang J, Li X, Guo M, Xing L, Cao X, et al. Crystallization and preliminary X-ray analysis of Escherichia coli RNase G. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009;65:586-8 pubmed publisher
    ..Purified recombinant E. coli RNase G, which has 497 amino acids, was crystallized in the cubic space group F432, with unit-cell parameters a = b = c = 219.84 A. X-ray diffraction data were collected to a resolution of 3.4 A. ..
  3. Zhou X, Meng X, Sun B. An EAL domain protein and cyclic AMP contribute to the interaction between the two quorum sensing systems in Escherichia coli. Cell Res. 2008;18:937-48 pubmed publisher
    ..These results indicate that signaling pathways that respond to important environmental cues, such as autoinducers and glucose, are linked together for their control in E. coli. ..
  4. Li X, Wei Z, Zhang M, Peng X, Yu G, Teng M, et al. Crystal structures of E. coli laccase CueO at different copper concentrations. Biochem Biophys Res Commun. 2007;354:21-6 pubmed
    ..In addition, an extra alpha-helix from Leu351 to Gly378 covers substrate biding pocket of CueO and might compromises the electron transfer from substrate to type I copper. ..
  5. Tang M, Wang S, Wang T, Zhao S, Wu Y, Wu L, et al. Mutational spectrum of the lacI gene in Escherichia coli K12 induced by low-energy ion beam. Mutat Res. 2006;602:163-9 pubmed
  6. Fan J, Wang D, Liang Z, Guo M, Teng M, Niu L. Maize uroporphyrinogen III methyltransferase: overexpression of the functional gene fragments in Escherichia coli and one-step purification. Protein Expr Purif. 2006;46:40-6 pubmed publisher
    ..This association slightly altered the protein secondary structure confirmed by circular dichroism assay...
  7. Xie C, Yao J, Pan R, Wu L, Yu Z. [Mutagenesis of ion beam implantation and identification of two new rifampicin resistance determining sites in rpoB gene in Escherichia coli]. Wei Sheng Wu Xue Bao. 2003;43:732-9 pubmed
    ..When dC1692 was replaced by dT, it resulted in a Pro564 is changed into a leucine. The synonymous mutation of the second site had been reported but the nucleotide substitution, dC-->dT, had not been identified before. ..
  8. Mu W, Zhang D, Xu L, Luo Z, Wang Y. Activity assay of His-tagged E. coli DNA photolyase by RP-HPLC and SE-HPLC. J Biochem Biophys Methods. 2005;63:111-24 pubmed
    ..The enzyme was found to be able to repair the cyclobutane pyrimidine dimer with the turnover rate of 2.4 dimers/photolyase molecule/min. ..