Experts and Doctors on methylation in Toronto, Ontario, Canada


Locale: Toronto, Ontario, Canada
Topic: methylation

Top Publications

  1. Ting K, Aitken K, Penna F, Samiei A, Sidler M, Jiang J, et al. Uropathogenic E. coli (UPEC) Infection Induces Proliferation through Enhancer of Zeste Homologue 2 (EZH2). PLoS ONE. 2016;11:e0149118 pubmed publisher
    ..Uropathogen-induced paracrine factors act epigenetically by altering expression of EZH2, which plays a key role in early host cell proliferative responses to infection. ..
  2. Gu Z, Jin K, Crabbe M, Zhang Y, Liu X, Huang Y, et al. Enrichment analysis of Alu elements with different spatial chromatin proximity in the human genome. Protein Cell. 2016;7:250-266 pubmed publisher
    ..Moreover, the GC-rich Alu sequence can bring high GC content and methylation flexibility to the regions with more distal chromatin contact, regulating the transcription of tissue-specific genes. ..
  3. Lissina E, Young B, Urbanus M, Guan X, Lowenson J, Hoon S, et al. A systems biology approach reveals the role of a novel methyltransferase in response to chemical stress and lipid homeostasis. PLoS Genet. 2011;7:e1002332 pubmed publisher
    ..This approach demonstrates the value of combining chemical genomics with other systems-based methods for characterizing proteins and elucidating previously unknown mechanisms of action of small molecule inhibitors. ..
  4. Wilson A, Garcia A, Jin L, Houle S. Radiotracer synthesis from [(11)C]-iodomethane: a remarkably simple captive solvent method. Nucl Med Biol. 2000;27:529-32 pubmed
  5. Boudrault C, Bazinet R, Kang J, Ma D. Cyclooxygenase-2 and n-6 PUFA are lower and DHA is higher in the cortex of fat-1 mice. Neurochem Int. 2010;56:585-9 pubmed publisher
    ..02), but cPLA(2) expression levels did not change. Our results suggest that this model could be used to investigate mechanisms by which n-3 PUFA regulate neuroinflammation. ..
  6. Mastronardi F, Tsui H, Winer S, Wood D, Selvanantham T, Galligan C, et al. Synergy between paclitaxel plus an exogenous methyl donor in the suppression of murine demyelinating diseases. Mult Scler. 2007;13:596-609 pubmed
    ..This report provides a mechanistic foundation for the development of a new therapeutic strategy in humans with MS. ..
  7. Religa T, Kay L. Optimal methyl labeling for studies of supra-molecular systems. J Biomol NMR. 2010;47:163-9 pubmed publisher
    ..Thus, labeling of supra-molecules with (13)CH(3) isotopomers remains the method of choice, but in applications where (13)CHD(2) moieties are required, sensitivity will in general not be limiting...
  8. Elango R, Humayun M, Turner J, Rafii M, Langos V, Ball R, et al. Total Sulfur Amino Acid Requirements Are Not Altered in Children with Chronic Renal Insufficiency, but Minimum Methionine Needs Are Increased. J Nutr. 2017;147:1954-1959 pubmed publisher
  9. Wolf A, Agnihotri S, Munoz D, Guha A. Developmental profile and regulation of the glycolytic enzyme hexokinase 2 in normal brain and glioblastoma multiforme. Neurobiol Dis. 2011;44:84-91 pubmed publisher
    ..Overall, our results demonstrate that proliferative states including the developing embryo and malignant gliomas, which rely on aerobic glycolysis, preferentially express the HK2 isoform, found to be regulated in part epigenetically. ..

More Information


  1. Walker E, Chang W, Hunkapiller J, Cagney G, Garcha K, Torchia J, et al. Polycomb-like 2 associates with PRC2 and regulates transcriptional networks during mouse embryonic stem cell self-renewal and differentiation. Cell Stem Cell. 2010;6:153-66 pubmed publisher
    ..We describe the role of PCL2 in both modulating transcription of ESC self-renewal genes in undifferentiated ESCs as well as developmental regulators during early commitment and differentiation. ..
  2. Guo Y, Nady N, Qi C, Allali Hassani A, Zhu H, Pan P, et al. Methylation-state-specific recognition of histones by the MBT repeat protein L3MBTL2. Nucleic Acids Res. 2009;37:2204-10 pubmed publisher
  3. De Luca V, Viggiano E, Dhoot R, Kennedy J, Wong A. Methylation and QTDT analysis of the 5-HT2A receptor 102C allele: analysis of suicidality in major psychosis. J Psychiatr Res. 2009;43:532-7 pubmed publisher
    ..This result suggests that methylation of the 102C allele does not influence completed suicide. ..
  4. Fish J, Matouk C, Rachlis A, Lin S, Tai S, D Abreo C, et al. The expression of endothelial nitric-oxide synthase is controlled by a cell-specific histone code. J Biol Chem. 2005;280:24824-38 pubmed
    ..Taken together, these findings provide strong evidence that the endothelial cell-specific expression of eNOS is controlled by cell-specific histone modifications. ..
  5. Sprangers R, Kay L. Quantitative dynamics and binding studies of the 20S proteasome by NMR. Nature. 2007;445:618-22 pubmed
    ..Our results establish that NMR spectroscopy can provide detailed insight into supra-molecular structures over an order of magnitude larger than those routinely studied using methodology that is generally applicable...
  6. Chan G, Fish J, Mawji I, Leung D, Rachlis A, Marsden P. Epigenetic basis for the transcriptional hyporesponsiveness of the human inducible nitric oxide synthase gene in vascular endothelial cells. J Immunol. 2005;175:3846-61 pubmed
    ..Collectively, our work defines a role for chromatin-based mechanisms in the control of human iNOS gene expression. ..
  7. Schuetz A, Allali Hassani A, Martin F, Loppnau P, Vedadi M, Bochkarev A, et al. Structural basis for molecular recognition and presentation of histone H3 by WDR5. EMBO J. 2006;25:4245-52 pubmed
    ..We propose a mechanism for the involvement of WDR5 in binding and presenting histone H3K4 for further methylation as a component of MLL complexes. ..
  8. Min J, Allali Hassani A, Nady N, Qi C, Ouyang H, Liu Y, et al. L3MBTL1 recognition of mono- and dimethylated histones. Nat Struct Mol Biol. 2007;14:1229-30 pubmed
    ..An unexpected mode of peptide-mediated dimerization suggests a possible mechanism for chromatin compaction by L3MBTL1. ..
  9. Blazer L, Lima Fernandes E, Gibson E, Eram M, Loppnau P, Arrowsmith C, et al. PR Domain-containing Protein 7 (PRDM7) Is a Histone 3 Lysine 4 Trimethyltransferase. J Biol Chem. 2016;291:13509-19 pubmed publisher
  10. Fu W, Liu N, Qiao Q, Wang M, Min J, Zhu B, et al. Structural Basis for Substrate Preference of SMYD3, a SET Domain-containing Protein Lysine Methyltransferase. J Biol Chem. 2016;291:9173-80 pubmed publisher
    ..The knowledge should be useful for the development of SMYD3 inhibitors in the fight against MAP3K2 and Ras-driven cancer. ..
  11. Dubuc A, Remke M, Korshunov A, Northcott P, Zhan S, Mendez Lago M, et al. Aberrant patterns of H3K4 and H3K27 histone lysine methylation occur across subgroups in medulloblastoma. Acta Neuropathol. 2013;125:373-84 pubmed publisher
    ..As PRC2-mediated aberrant methylation of H3K27 has recently been targeted for therapy in other diseases, it represents an actionable target for a substantial percentage of medulloblastoma patients with aggressive forms of the disease. ..
  12. Wu H, Zeng H, Lam R, Tempel W, Amaya M, Xu C, et al. Structural and histone binding ability characterizations of human PWWP domains. PLoS ONE. 2011;6:e18919 pubmed publisher
    ..Please note that a web plugin is required to access this enhanced functionality. Instructions for the installation and use of the web plugin are available in Text S1. ..
  13. Adams Cioaba M, Guo Y, Bian C, Amaya M, Lam R, Wasney G, et al. Structural studies of the tandem Tudor domains of fragile X mental retardation related proteins FXR1 and FXR2. PLoS ONE. 2010;5:e13559 pubmed publisher
    ..Please note that a web plugin is required to access this enhanced functionality. Instructions for the installation and use of the web plugin are available in Text S1. ..
  14. Xu C, Bian C, Yang W, Galka M, Ouyang H, Chen C, et al. Binding of different histone marks differentially regulates the activity and specificity of polycomb repressive complex 2 (PRC2). Proc Natl Acad Sci U S A. 2010;107:19266-71 pubmed publisher
    ..In addition to determining the molecular basis of EED-methyllysine recognition, our work provides the biochemical characterization of how the activity of a histone methyltransferase is oppositely regulated by two histone marks. ..
  15. Moscarello M, Mastronardi F, Wood D. The role of citrullinated proteins suggests a novel mechanism in the pathogenesis of multiple sclerosis. Neurochem Res. 2007;32:251-6 pubmed
    ..We propose that citrullinated MBP, resulting from elevated levels of PAD 2 represents an important biochemical pathway in the pathogenesis of MS. ..
  16. Mastronardi F, Noor A, Wood D, Paton T, Moscarello M. Peptidyl argininedeiminase 2 CpG island in multiple sclerosis white matter is hypomethylated. J Neurosci Res. 2007;85:2006-16 pubmed
  17. Mansouri S, Wang S, Frappier L. A role for the nucleosome assembly proteins TAF-I? and NAP1 in the activation of BZLF1 expression and Epstein-Barr virus reactivation. PLoS ONE. 2013;8:e63802 pubmed publisher
    ..The results indicate that TAF-I? promotes BZLF1 expression and subsequent lytic infection by affecting chromatin at the BZLF1 promoter. ..
  18. Lee J, Luong W, Huang D, Cornell K, Riscoe M, Howell P. Mutational analysis of a nucleosidase involved in quorum-sensing autoinducer-2 biosynthesis. Biochemistry. 2005;44:11049-57 pubmed
    ..Furthermore, mutation of Arg193 to alanine shows that the nucleophilic water is able to direct its attack without assistance from the enzyme. This mutagenesis study has allowed a reevaluation of the catalytic mechanism. ..
  19. Walters B, Mercaldo V, Gillon C, Yip M, Neve R, BOYCE F, et al. The Role of The RNA Demethylase FTO (Fat Mass and Obesity-Associated) and mRNA Methylation in Hippocampal Memory Formation. Neuropsychopharmacology. 2017;42:1502-1510 pubmed publisher
    ..Together, these results show the importance of FTO during memory formation and, furthermore, implicate mRNA modification and epi-transcriptomics as novel regulators of memory formation. ..
  20. Krogan N, Dover J, Wood A, Schneider J, Heidt J, Boateng M, et al. The Paf1 complex is required for histone H3 methylation by COMPASS and Dot1p: linking transcriptional elongation to histone methylation. Mol Cell. 2003;11:721-9 pubmed
    ..Collectively, our results suggest that the Paf1 complex is required for histone H3 methylation, therefore linking transcriptional elongation to chromatin methylation. ..
  21. Arrowsmith C, Bountra C, Fish P, Lee K, Schapira M. Epigenetic protein families: a new frontier for drug discovery. Nat Rev Drug Discov. 2012;11:384-400 pubmed publisher
    ..In this article, we discuss the known links with disease, basic molecular mechanisms of action and recent progress in the pharmacological modulation of each class of proteins. ..
  22. Rugg Gunn P, Cox B, Ralston A, Rossant J. Distinct histone modifications in stem cell lines and tissue lineages from the early mouse embryo. Proc Natl Acad Sci U S A. 2010;107:10783-90 pubmed publisher
    ..These studies indicate that histone modification mechanisms may differ between early embryo lineages and emphasize the importance of examining in vivo and in vitro progenitor cells. ..
  23. Rival Gervier S, Lo M, Khattak S, Pasceri P, Lorincz M, Ellis J. Kinetics and epigenetics of retroviral silencing in mouse embryonic stem cells defined by deletion of the D4Z4 element. Mol Ther. 2013;21:1536-50 pubmed publisher
    ..We conclude that vectors with both 3'D4Z4 and HS4 insulator elements fully block silencing, and may have unprecedented utility for gene transfer applications that require long-term gene expression in pluripotent stem (PS) cells. ..
  24. Eryilmaz J, Pan P, Amaya M, Allali Hassani A, Dong A, Adams Cioaba M, et al. Structural studies of a four-MBT repeat protein MBTD1. PLoS ONE. 2009;4:e7274 pubmed publisher
    ..Please note that a web plugin is required to access this enhanced functionality. Instructions for the installation and use of the web plugin are available in Text S1. ..