Experts and Doctors on carbohydrate sequence in Guelph, Ontario, Canada

Summary

Locale: Guelph, Ontario, Canada
Topic: carbohydrate sequence

Top Publications

  1. Yethon J, Heinrichs D, Monteiro M, Perry M, Whitfield C. Involvement of waaY, waaQ, and waaP in the modification of Escherichia coli lipopolysaccharide and their role in the formation of a stable outer membrane. J Biol Chem. 1998;273:26310-6 pubmed
    ..Only mutation of waaP resulted in hypersensitivity to novobiocin and sodium dodecyl sulfate, a characteristic of deep-rough mutations. ..
  2. Yethon J, Vinogradov E, Perry M, Whitfield C. Mutation of the lipopolysaccharide core glycosyltransferase encoded by waaG destabilizes the outer membrane of Escherichia coli by interfering with core phosphorylation. J Bacteriol. 2000;182:5620-3 pubmed
    ..Structural analyses of waaG mutant lipopolysaccharide showed that the cause for this phenotype was a decrease in core phosphorylation, an unexpected side effect of the waaG mutation. ..
  3. Kaniuk N, Monteiro M, Parker C, Whitfield C. Molecular diversity of the genetic loci responsible for lipopolysaccharide core oligosaccharide assembly within the genus Salmonella. Mol Microbiol. 2002;46:1305-18 pubmed
  4. Deutschmann R, Boncheff A, Daraban L, MacInnes J, Monteiro M. Common sialylated glycan in Actinobacillus suis. Glycobiology. 2010;20:1227-32 pubmed publisher
    ..The ubiquitous expression of this sialylated glycan suggests that this carbohydrate may play an important role in the survival of A. suis in the host...
  5. Clarke B, Richards M, Greenfield L, Hou D, Lowary T, Whitfield C. In vitro reconstruction of the chain termination reaction in biosynthesis of the Escherichia coli O9a O-polysaccharide: the chain-length regulator, WbdD, catalyzes the addition of methyl phosphate to the non-reducing terminus of the growing glycan. J Biol Chem. 2011;286:41391-401 pubmed publisher
  6. Auzanneau F, Borrelli S, Pinto B. Synthesis and immunological activity of an oligosaccharide-conjugate as a vaccine candidate against Group A Streptococcus. Bioorg Med Chem Lett. 2013;23:6038-42 pubmed publisher
    ..The immunogenicity in mice is similar to that obtained with a native CWPS-TT conjugate, validating the glycoconjugate as a vaccine candidate against GAS infections. ..
  7. Walsh A, Matewish M, Burrows L, Monteiro M, Perry M, Lam J. Lipopolysaccharide core phosphates are required for viability and intrinsic drug resistance in Pseudomonas aeruginosa. Mol Microbiol. 2000;35:718-27 pubmed
    ..The inner core phosphates of P. aeruginosa appear to be playing a key role in the intrinsic drug resistance of this bacterium. ..
  8. Yethon J, Whitfield C. Purification and characterization of WaaP from Escherichia coli, a lipopolysaccharide kinase essential for outer membrane stability. J Biol Chem. 2001;276:5498-504 pubmed
    ..These data are the first proof that WaaP is indeed an LPS kinase. Further, site-directed mutagenesis of a predicted catalytic residue suggests that WaaP shares a common mechanism of action with eukaryotic protein kinases. ..
  9. Frirdich E, Lindner B, Holst O, Whitfield C. Overexpression of the waaZ gene leads to modification of the structure of the inner core region of Escherichia coli lipopolysaccharide, truncation of the outer core, and reduction of the amount of O polysaccharide on the cell surface. J Bacteriol. 2003;185:1659-71 pubmed
    ..Novel truncated LPS structures, lacking up to 3 hexoses from the outer core, resulted from WaaZ overexpression. The truncated molecules also contained a KdoIII residue not normally found in the R1 core. ..
  10. Goodridge L, Gallaccio A, Griffiths M. Morphological, host range, and genetic characterization of two coliphages. Appl Environ Microbiol. 2003;69:5364-71 pubmed
    ..The wide host ranges of phages AR1 and LG1 suggest that they may be useful as biocontrol, therapeutic, or diagnostic agents to control and detect the prevalence of E. coli in animals and food...

Detail Information

Publications13

  1. Yethon J, Heinrichs D, Monteiro M, Perry M, Whitfield C. Involvement of waaY, waaQ, and waaP in the modification of Escherichia coli lipopolysaccharide and their role in the formation of a stable outer membrane. J Biol Chem. 1998;273:26310-6 pubmed
    ..Only mutation of waaP resulted in hypersensitivity to novobiocin and sodium dodecyl sulfate, a characteristic of deep-rough mutations. ..
  2. Yethon J, Vinogradov E, Perry M, Whitfield C. Mutation of the lipopolysaccharide core glycosyltransferase encoded by waaG destabilizes the outer membrane of Escherichia coli by interfering with core phosphorylation. J Bacteriol. 2000;182:5620-3 pubmed
    ..Structural analyses of waaG mutant lipopolysaccharide showed that the cause for this phenotype was a decrease in core phosphorylation, an unexpected side effect of the waaG mutation. ..
  3. Kaniuk N, Monteiro M, Parker C, Whitfield C. Molecular diversity of the genetic loci responsible for lipopolysaccharide core oligosaccharide assembly within the genus Salmonella. Mol Microbiol. 2002;46:1305-18 pubmed
  4. Deutschmann R, Boncheff A, Daraban L, MacInnes J, Monteiro M. Common sialylated glycan in Actinobacillus suis. Glycobiology. 2010;20:1227-32 pubmed publisher
    ..The ubiquitous expression of this sialylated glycan suggests that this carbohydrate may play an important role in the survival of A. suis in the host...
  5. Clarke B, Richards M, Greenfield L, Hou D, Lowary T, Whitfield C. In vitro reconstruction of the chain termination reaction in biosynthesis of the Escherichia coli O9a O-polysaccharide: the chain-length regulator, WbdD, catalyzes the addition of methyl phosphate to the non-reducing terminus of the growing glycan. J Biol Chem. 2011;286:41391-401 pubmed publisher
  6. Auzanneau F, Borrelli S, Pinto B. Synthesis and immunological activity of an oligosaccharide-conjugate as a vaccine candidate against Group A Streptococcus. Bioorg Med Chem Lett. 2013;23:6038-42 pubmed publisher
    ..The immunogenicity in mice is similar to that obtained with a native CWPS-TT conjugate, validating the glycoconjugate as a vaccine candidate against GAS infections. ..
  7. Walsh A, Matewish M, Burrows L, Monteiro M, Perry M, Lam J. Lipopolysaccharide core phosphates are required for viability and intrinsic drug resistance in Pseudomonas aeruginosa. Mol Microbiol. 2000;35:718-27 pubmed
    ..The inner core phosphates of P. aeruginosa appear to be playing a key role in the intrinsic drug resistance of this bacterium. ..
  8. Yethon J, Whitfield C. Purification and characterization of WaaP from Escherichia coli, a lipopolysaccharide kinase essential for outer membrane stability. J Biol Chem. 2001;276:5498-504 pubmed
    ..These data are the first proof that WaaP is indeed an LPS kinase. Further, site-directed mutagenesis of a predicted catalytic residue suggests that WaaP shares a common mechanism of action with eukaryotic protein kinases. ..
  9. Frirdich E, Lindner B, Holst O, Whitfield C. Overexpression of the waaZ gene leads to modification of the structure of the inner core region of Escherichia coli lipopolysaccharide, truncation of the outer core, and reduction of the amount of O polysaccharide on the cell surface. J Bacteriol. 2003;185:1659-71 pubmed
    ..Novel truncated LPS structures, lacking up to 3 hexoses from the outer core, resulted from WaaZ overexpression. The truncated molecules also contained a KdoIII residue not normally found in the R1 core. ..
  10. Goodridge L, Gallaccio A, Griffiths M. Morphological, host range, and genetic characterization of two coliphages. Appl Environ Microbiol. 2003;69:5364-71 pubmed
    ..The wide host ranges of phages AR1 and LG1 suggest that they may be useful as biocontrol, therapeutic, or diagnostic agents to control and detect the prevalence of E. coli in animals and food...
  11. King J, Mulrooney E, Vinogradov E, Kneidinger B, Mead K, Lam J. lfnA from Pseudomonas aeruginosa O12 and wbuX from Escherichia coli O145 encode membrane-associated proteins and are required for expression of 2,6-dideoxy-2-acetamidino-L-galactose in lipopolysaccharide O antigen. J Bacteriol. 2008;190:1671-9 pubmed
    ..Both proteins retained their activity when fused to a His(6) tag and localized to the membrane fraction. These data will assist future biochemical investigation of this pathway. ..
  12. Poon K, Westman E, Vinogradov E, Jin S, Lam J. Functional characterization of MigA and WapR: putative rhamnosyltransferases involved in outer core oligosaccharide biosynthesis of Pseudomonas aeruginosa. J Bacteriol. 2008;190:1857-65 pubmed publisher
    ..In contrast, uncapped core OS from the migA mutant lacked alpha-1,6-L-Rha. These results provide evidence that WapR is the alpha-1,3-rhamnosyltransferase, while MigA is the alpha-1,6-rhamnosyltransferase...
  13. Kropinski A, Sulakvelidze A, Konczy P, Poppe C. Salmonella phages and prophages--genomics and practical aspects. Methods Mol Biol. 2007;394:133-75 pubmed publisher
    ..The only member of the T7-like group is SP6. The properties of each of these phages are discussed, along with their role as agents of genetic exchange and as therapeutic agents and their involvement in phage typing...