Genomes and Genes
Experts and Doctors on caenorhabditis elegans proteins in Toronto, Ontario, Canada
Locale: Toronto, Ontario, Canada
Topic: caenorhabditis elegans proteins
- Bohdanowicz M, Grinstein S. Vesicular traffic: a Rab SANDwich. Curr Biol. 2010;20:R311-4 pubmed publisher..Recent studies in Caenorhabditis elegans have now uncovered a new protein complex that connects Rab5 to Rab7. ..
- Koon J, Kubiseski T. Developmental arrest of Caenorhabditis elegans BRAP-2 mutant exposed to oxidative stress is dependent on BRC-1. J Biol Chem. 2010;285:13437-43 pubmed publisher..Our findings demonstrate that BRAP-2 is necessary for preventing an inappropriate response to elevated levels of reactive oxygen species by countering premature activation of BRC-1 and CKI-1. ..
- Luciani G, Magomedova L, Puckrin R, Urbanus M, Wallace I, Giaever G, et al. Dafadine inhibits DAF-9 to promote dauer formation and longevity of Caenorhabditis elegans. Nat Chem Biol. 2011;7:891-3 pubmed publisher..This molecule (called dafadine) also inhibits the mammalian ortholog of DAF-9(CYP27A1), suggesting that dafadine can be used to interrogate developmental control and longevity in other animals. ..
- Schachter H. Protein glycosylation lessons from Caenorhabditis elegans. Curr Opin Struct Biol. 2004;14:607-16 pubmed..Recent studies have focused on N-glycosylation, lumenal nucleoside diphosphatases, the resistance of C. elegans to a bacterial toxin and infections, fucosylation and proteoglycans. ..
- Lau B, Ward W, Kang J, Ma D. Femur EPA and DHA are correlated with femur biomechanical strength in young fat-1 mice. J Nutr Biochem. 2009;20:453-61 pubmed publisher..In conclusion, the results of the present study suggest that n-3 PUFA have a favorable effect on mineral accumulation and functional measures of bone in fat-1 mice at young adulthood. ..
- Winer S, Astsaturov I, Gaedigk R, Hammond McKibben D, Pilon M, Song A, et al. ICA69(null) nonobese diabetic mice develop diabetes, but resist disease acceleration by cyclophosphamide. J Immunol. 2002;168:475-82 pubmed..CY-accelerated diabetes involves not only ablation of lymphoid cells, but ICA69-dependent drug toxicity in beta cells that boosts autoreactivity in the regenerating lymphoid system. ..
- Hung W, Hwang C, Gao S, Liao E, Chitturi J, Wang Y, et al. Attenuation of insulin signalling contributes to FSN-1-mediated regulation of synapse development. EMBO J. 2013;32:1745-60 pubmed publisher..We propose that FSN-1 may negatively regulate insulin/IGF signalling, in part, through EGL-3-dependent insulin-like ligand processing. ..
- Xin X, Gfeller D, Cheng J, Tonikian R, Sun L, Guo A, et al. SH3 interactome conserves general function over specific form. Mol Syst Biol. 2013;9:652 pubmed publisher..Nevertheless, orthologous SH3 domain-mediated interactions are highly rewired. Our results suggest a model of network evolution where general function of the SH3 domain network is conserved over its specific form. ..
- Schvarzstein M, Spence A. The C. elegans sex-determining GLI protein TRA-1A is regulated by sex-specific proteolysis. Dev Cell. 2006;11:733-40 pubmed..elegans sex determination. ..
- Akay A, Di Doménico T, Suen K, Nabih A, Parada G, Larance M, et al. The Helicase Aquarius/EMB-4 Is Required to Overcome Intronic Barriers to Allow Nuclear RNAi Pathways to Heritably Silence Transcription. Dev Cell. 2017;42:241-255.e6 pubmed publisher..Thus, Aquarius and HRDE-1 act as gatekeepers coordinating gene expression and genome defense. ..
- Alexander M, Selman G, Seetharaman A, Chan K, D Souza S, Byrne A, et al. MADD-2, a homolog of the Opitz syndrome protein MID1, regulates guidance to the midline through UNC-40 in Caenorhabditis elegans. Dev Cell. 2010;18:961-72 pubmed publisher..The analogous phenotypes that result from MADD-2 and MID1 mutations suggest that C1-TRIM proteins may have a conserved biological role in midline-oriented developmental events. ..
- Donoviel D, Donoviel M, Fan E, Hadjantonakis A, Bernstein A. Cloning and characterization of Sel-1l, a murine homolog of the C. elegans sel-1 gene. Mech Dev. 1998;78:203-7 pubmed..5 and E17.5 in the acini of the pancreas, and moderate in the epithelial cells of the gut villi. We localized the SEL-1L protein to the cytosol, possibly in intracellular vesicles, in a beta-islet-derived tumor cell line (RinM). ..
- Lant B, Derry W. Methods for detection and analysis of apoptosis signaling in the C. elegans germline. Methods. 2013;61:174-82 pubmed publisher..We also present the limitations of these methods, and suggest complimentary techniques in order that researchers new to the field can comprehensively assess apoptosis phenotypes in the C. elegans germline. ..
- Yeh E, Kawano T, Ng S, Fetter R, Hung W, Wang Y, et al. Caenorhabditis elegans innexins regulate active zone differentiation. J Neurosci. 2009;29:5207-17 pubmed publisher..Our mosaic analyses, electron microscopy, as well as expression studies suggest a novel and likely nonjunctional role of specific innexins in active zone differentiation in addition to gap junction formations. ..
- Steven R, Zhang L, Culotti J, Pawson T. The UNC-73/Trio RhoGEF-2 domain is required in separate isoforms for the regulation of pharynx pumping and normal neurotransmission in C. elegans. Genes Dev. 2005;19:2016-29 pubmed
- Harris K, Tepass U. Cdc42 and vesicle trafficking in polarized cells. Traffic. 2010;11:1272-9 pubmed publisher..The recent discovery that the Par polarity complex co-operates with Cdc42 in the regulation of endocytosis and recycling opens exciting possibilities for the integration of polarity protein function and endocytotic machinery. ..
- Kawano T, Zheng H, Merz D, Kohara Y, Tamai K, Nishiwaki K, et al. C. elegans mig-6 encodes papilin isoforms that affect distinct aspects of DTC migration, and interacts genetically with mig-17 and collagen IV. Development. 2009;136:1433-42 pubmed publisher..Genetic data also suggest that MIG-6S and non-fibrillar network collagen IV play antagonistic roles to ensure normal phase 2 DTC guidance. ..
- Suo S, Kimura Y, Van Tol H. Starvation induces cAMP response element-binding protein-dependent gene expression through octopamine-Gq signaling in Caenorhabditis elegans. J Neurosci. 2006;26:10082-90 pubmed..The results show that the endogenous octopamine system in C. elegans is activated by starvation and that different environmental stimuli can activate CREB through G alpha(q). ..
- Steven R, Kubiseski T, Zheng H, Kulkarni S, Mancillas J, Ruiz Morales A, et al. UNC-73 activates the Rac GTPase and is required for cell and growth cone migrations in C. elegans. Cell. 1998;92:785-95 pubmed..Our results suggest that UNC-73 acts cell autonomously in a protein complex to regulate actin dynamics during cell and growth cone migrations. ..
- Dalpé G, Zheng H, Brown L, Culotti J. Semaphorin-1 and netrin signal in parallel and permissively to position the male ray 1 sensillum in Caenorhabditis elegans. Genetics. 2012;192:959-71 pubmed publisher..Several lines of evidence indicate that SMP-1 and UNC-6 function permissively in the context of ray 1 positioning. ..
- Johnston W, Krizus A, Dennis J. Eggshell chitin and chitin-interacting proteins prevent polyspermy in C. elegans. Curr Biol. 2010;20:1932-7 pubmed publisher..Together, our results show that eggshell chitin is required to prevent polyspermy in C. elegans, in addition to its previously reported requirement in polar body extrusion and polarization of the zygote. ..
- Gao M, Liao E, Yu B, Wang Y, Zhen M, Derry W. The SCF FSN-1 ubiquitin ligase controls germline apoptosis through CEP-1/p53 in C. elegans. Cell Death Differ. 2008;15:1054-62 pubmed publisher..Our results uncover a novel role for the SCF(FSN-1) E3 ubiquitin ligase in the regulation of cep-1-dependent germ cell apoptosis. ..
- Ito S, Greiss S, Gartner A, Derry W. Cell-nonautonomous regulation of C. elegans germ cell death by kri-1. Curr Biol. 2010;20:333-8 pubmed publisher..Interestingly, we find that kri-1 regulates cell death in a cell-nonautonomous manner, revealing a novel regulatory role for nondying cells in eliciting cell death in response to DNA damage. ..
- Sassi H, Renihan S, Spence A, Cooperstock R. Gene CATCHR--gene cloning and tagging for Caenorhabditis elegans using yeast homologous recombination: a novel approach for the analysis of gene expression. Nucleic Acids Res. 2005;33:e163 pubmed..Mutant rescue assays demonstrate that Gene Catchr-generated transgenes are functional. Our results validate the use of Gene Catchr as a valuable tool to study spatiotemporal gene expression. ..
- Zhang Y, Kubiseski T. Caenorhabditis elegans wsp-1 regulation of synaptic function at the neuromuscular junction. J Biol Chem. 2010;285:23040-6 pubmed publisher..These results provide genetic and pharmacological evidence that WSP-1 plays an essential role to stabilize the actin cytoskeleton at the neuronal active zone of the neuromuscular junction to restrain synaptic vesicle release. ..
- Seetharaman A, Selman G, Puckrin R, Barbier L, Wong E, D Souza S, et al. MADD-4 is a secreted cue required for midline-oriented guidance in Caenorhabditis elegans. Dev Cell. 2011;21:669-80 pubmed publisher..The biological role of MADD-4 orthologs, including ADAMTSL1 and 3 in mammals, is unknown. MADD-4 may therefore represent the founding member of a family of guidance proteins. ..
- Johnson C, Spence A. Epigenetic licensing of germline gene expression by maternal RNA in C. elegans. Science. 2011;333:1311-4 pubmed publisher..This mechanism may contribute to protecting the identity and integrity of the germ line. ..
- Ross A, Li M, Yu B, Gao M, Derry W. The EEL-1 ubiquitin ligase promotes DNA damage-induced germ cell apoptosis in C. elegans. Cell Death Differ. 2011;18:1140-9 pubmed publisher..Although ee1-1 mutants exhibit hypersensitivity to genotoxic stress they do not appear to be defective in DNA repair, suggesting a distinct role for EEL-1 in promoting damage-induced apoptosis in the germline. ..
- Wang W, Bouhours M, Gracheva E, Liao E, Xu K, Sengar A, et al. ITSN-1 controls vesicle recycling at the neuromuscular junction and functions in parallel with DAB-1. Traffic. 2008;9:742-54 pubmed publisher..These results show for the first time that intersectin and Eps15 proteins function in the same genetic pathway, and appear to function synergistically with the clathrin-coat-associated sorting protein, Disabled, for viability. ..
- Marengere L, Songyang Z, Gish G, Schaller M, Parsons J, Stern M, et al. SH2 domain specificity and activity modified by a single residue. Nature. 1994;369:502-5 pubmed..These results identify a residue that can modify SH2 selectivity, and indicate that the biological activity of an SH2 domain correlates with its binding specificity. ..
- Chan S, Zheng H, Su M, Wilk R, Killeen M, Hedgecock E, et al. UNC-40, a C. elegans homolog of DCC (Deleted in Colorectal Cancer), is required in motile cells responding to UNC-6 netrin cues. Cell. 1996;87:187-95 pubmed..Together with the recent report that DCC is a netrin receptor in vertebrates, our results suggest that UNC-40 is a component of UNC-6 receptors on motile cells. ..
- Mehra A, Gaudet J, Heck L, Kuwabara P, Spence A. Negative regulation of male development in Caenorhabditis elegans by a protein-protein interaction between TRA-2A and FEM-3. Genes Dev. 1999;13:1453-63 pubmed..When the balance favors FEM-3, it acts through or with the other FEM proteins to promote male cell fates. ..
- Wang X, Roy P, Holland S, Zhang L, Culotti J, Pawson T. Multiple ephrins control cell organization in C. elegans using kinase-dependent and -independent functions of the VAB-1 Eph receptor. Mol Cell. 1999;4:903-13 pubmed..elegans. ..
- Pilon M, Peng X, Spence A, Plasterk R, Dosch H. The diabetes autoantigen ICA69 and its Caenorhabditis elegans homologue, ric-19, are conserved regulators of neuroendocrine secretion. Mol Biol Cell. 2000;11:3277-88 pubmed
- Wedeles C, Wu M, Claycomb J. A multitasking Argonaute: exploring the many facets of C. elegans CSR-1. Chromosome Res. 2013;21:573-86 pubmed publisher..elegans Argonaute out of 24 family members in total. Here, we summarize the current understanding of CSR-1 functions in the worm, with emphasis on the chromatin-directed activities of this ever-intriguing Argonaute. ..
- Morrison G, van der Kooy D. A mutation in the AMPA-type glutamate receptor, glr-1, blocks olfactory associative and nonassociative learning in Caenorhabditis elegans. Behav Neurosci. 2001;115:640-9 pubmed..The results suggest that although associative and nonassociative learning can be genetically dissociated (lrn-1 and lrn-2), they also share some common molecular processes, including glr-1-mediated neurotransmission. ..
- Warren C, Krizus A, Roy P, Culotti J, Dennis J. The Caenorhabditis elegans gene, gly-2, can rescue the N-acetylglucosaminyltransferase V mutation of Lec4 cells. J Biol Chem. 2002;277:22829-38 pubmed..However, no overt phenotypes were observed in animals homozygous for deletion alleles of gly-2. ..
- Quevedo C, Kaplan D, Derry W. AKT-1 regulates DNA-damage-induced germline apoptosis in C. elegans. Curr Biol. 2007;17:286-92 pubmed..Finally, we show that AKT-1 regulates apoptosis but not cell-cycle progression downstream of the HUS-1/MRT-2 branch of the DNA damage checkpoint. ..
- Mok C, Healey M, Shekhar T, Leroux M, Heon E, Zhen M. Mutations in a guanylate cyclase GCY-35/GCY-36 modify Bardet-Biedl syndrome-associated phenotypes in Caenorhabditis elegans. PLoS Genet. 2011;7:e1002335 pubmed publisher..We propose that a misregulation of cGMP signalling, which underlies developmental and some behavioural defects of C. elegans bbs mutants, may also contribute to some BBS features in other organisms. ..
- Dalpé G, Tarsitano M, Persico M, Zheng H, Culotti J. C. elegans PVF-1 inhibits permissive UNC-40 signalling through CED-10 GTPase to position the male ray 1 sensillum. Development. 2013;140:4020-30 pubmed publisher..These data report the first case of VEGF-induced inhibition of the netrin signalling and a molecular conservation of VEGF function from worms to humans. ..
- Hung W, Wang Y, Chitturi J, Zhen M. A Caenorhabditis elegans developmental decision requires insulin signaling-mediated neuron-intestine communication. Development. 2014;141:1767-79 pubmed publisher..Under adverse conditions, a switch in the agonistic-antagonistic ILP balance activates intestinal DAF-16, which commits animals to diapause. ..
- Florica R, Hipolito V, Bautista S, Anvari H, Rapp C, El Rass S, et al. The ENU-3 protein family members function in the Wnt pathway parallel to UNC-6/Netrin to promote motor neuron axon outgrowth in C. elegans. Dev Biol. 2017;430:249-261 pubmed publisher..We conclude that the ENU-3 family proteins function in a pathway parallel to the UNC-6/Netrin pathway for motor neuron axon outgrowth, most likely in the Wnt pathway. ..
- Tonikian R, Zhang Y, Sazinsky S, Currell B, Yeh J, Reva B, et al. A specificity map for the PDZ domain family. PLoS Biol. 2008;6:e239 pubmed publisher..These findings indicate that many viruses produce PDZ ligands that disrupt host protein complexes for their own benefit, and that highly pathogenic strains target PDZ domains involved in cell polarity and growth. ..
- Cook K, Kazan H, Zuberi K, Morris Q, Hughes T. RBPDB: a database of RNA-binding specificities. Nucleic Acids Res. 2011;39:D301-8 pubmed publisher..Users can also use RBPDB to scan sequences for RBP-binding sites. RBPDB is freely available, without registration at http://rbpdb.ccbr.utoronto.ca/. ..
- Kim J, Hung W, Narbonne P, Roy R, Zhen M. C. elegans STRADalpha and SAD cooperatively regulate neuronal polarity and synaptic organization. Development. 2010;137:93-102 pubmed publisher..Our findings suggest that instead of a single, linear genetic pathway, STRADalpha and LKB1 regulate neuronal development through multiple effectors that are shared in some cellular contexts but distinct in others. ..
- Leung Hagesteijn C, Spence A, Stern B, Zhou Y, Su M, Hedgecock E, et al. UNC-5, a transmembrane protein with immunoglobulin and thrombospondin type 1 domains, guides cell and pioneer axon migrations in C. elegans. Cell. 1992;71:289-99 pubmed..Mosaic analysis shows that unc-5 acts in migrating cells and pioneering neurons. We propose that UNC-5 is a transmembrane receptor expressed on the surface of motile cells and growth cones to guide dorsal movements. ..
- Dixon S, Alexander M, Chan K, Roy P. Insulin-like signaling negatively regulates muscle arm extension through DAF-12 in Caenorhabditis elegans. Dev Biol. 2008;318:153-61 pubmed publisher..We conclude that supernumerary muscle arms are a novel dauer-specific modification that may facilitate some aspect of dauer behavior. ..
- Fujisawa K, Wrana J, Culotti J. The slit receptor EVA-1 coactivates a SAX-3/Robo mediated guidance signal in C. elegans. Science. 2007;317:1934-8 pubmed..Double mutants of eva-1 or slt-1 with sax-3 mutations suggest that SAX-3 can (when slt-1 or eva-1 function is reduced) inhibit a parallel-acting guidance mechanism, which involves UNC-40/deleted in colorectal cancer. ..
- Zheng H, Coudiere L, Camia C, Colavita A, Culotti J, Merz D. C. elegans seu-1 encodes novel nuclear proteins that regulate responses to UNC-6/netrin guidance cues. Dev Biol. 2007;310:44-53 pubmed..These results implicate nuclear SEU-1 in the interpretation of UNC-6/netrin directional information by migrating growth cones and cells. ..
- Sanyal S, Wintle R, Kindt K, Nuttley W, Arvan R, Fitzmaurice P, et al. Dopamine modulates the plasticity of mechanosensory responses in Caenorhabditis elegans. EMBO J. 2004;23:473-82 pubmed..The tyrosine hydroxylase-deficient C. elegans mutant (cat-2) also displays these specific behavioral deficits. These observations provide genetic evidence that dopamine signaling modulates behavioral plasticity in C. elegans. ..
- Lin D, Edwards A, Fawcett J, Mbamalu G, Scott J, Pawson T. A mammalian PAR-3-PAR-6 complex implicated in Cdc42/Rac1 and aPKC signalling and cell polarity. Nat Cell Biol. 2000;2:540-7 pubmed..In vitro, mPAR-3 acts as a substrate and an inhibitor of aPKC. We conclude that mPAR-3 and mPAR-6 have a scaffolding function, coordinating the activities of several signalling proteins that are implicated in mammalian cell polarity. ..
- Merz D, Alves G, Kawano T, Zheng H, Culotti J. UNC-52/perlecan affects gonadal leader cell migrations in C. elegans hermaphrodites through alterations in growth factor signaling. Dev Biol. 2003;256:173-86 pubmed..We propose that UNC-52 serves dual roles in C. elegans larval development in the maintenance of muscle structure and the regulation of growth factor-like signaling pathways. ..
- Killeen M, Tong J, Krizus A, Steven R, Scott I, Pawson T, et al. UNC-5 function requires phosphorylation of cytoplasmic tyrosine 482, but its UNC-40-independent functions also require a region between the ZU-5 and death domains. Dev Biol. 2002;251:348-66 pubmed..Our data also show that part of the ZU-5 motif is required for UNC-40-independent signaling of UNC-5. ..
- Lum D, Kuwabara P, Zarkower D, Spence A. Direct protein-protein interaction between the intracellular domain of TRA-2 and the transcription factor TRA-1A modulates feminizing activity in C. elegans. Genes Dev. 2000;14:3153-65 pubmed..We further show that tagged derivatives of the intracellular domain of TRA-2 localize to the nucleus, supporting the hypothesis that this domain is capable of modulating TRA-1A activity in a manner reminiscent of Notch and Su(H). ..
- Tyc K, Nabih A, Wu M, Wedeles C, Sobotka J, Claycomb J. The Conserved Intron Binding Protein EMB-4 Plays Differential Roles in Germline Small RNA Pathways of C. elegans. Dev Cell. 2017;42:256-270.e6 pubmed publisher..These data suggest that EMB-4 could contribute to a molecular signature that distinguishes the targets of these two germline small RNA pathways. ..
- Colavita A, Culotti J. Suppressors of ectopic UNC-5 growth cone steering identify eight genes involved in axon guidance in Caenorhabditis elegans. Dev Biol. 1998;194:72-85 pubmed..Several of these mutations cause axon guidance defects similar to those found in unc-5 mutants. We propose that some or all of these genes may function in a developmentally important unc-5 signaling pathway. ..
- Roy P, Zheng H, Warren C, Culotti J. mab-20 encodes Semaphorin-2a and is required to prevent ectopic cell contacts during epidermal morphogenesis in Caenorhabditis elegans. Development. 2000;127:755-67 pubmed..These phenotypic traits are explained by the formation of inappropriate contacts between cells of similar type and suggest that Ce-Sema-2a may normally prevent formation or stabilization of ectopic adhesive contacts between these cells. ..
- Meng J, Ma X, Tao H, Jin X, Witvliet D, Mitchell J, et al. Myrf ER-Bound Transcription Factors Drive C.Â elegans Synaptic Plasticity via Cleavage-Dependent Nuclear Translocation. Dev Cell. 2017;41:180-194.e7 pubmed publisher..MYRF-1 and MYRF-2 are the first genes identified to be indispensable for promoting synaptic rewiring in C.Â elegans. These findings reveal a molecular mechanism underlying synaptic rewiring and developmental circuit plasticity. ..
- Kubiseski T, Culotti J, Pawson T. Functional analysis of the Caenorhabditis elegans UNC-73B PH domain demonstrates a role in activation of the Rac GTPase in vitro and axon guidance in vivo. Mol Cell Biol. 2003;23:6823-35 pubmed..These results suggest that the UNC-73B PH domain plays distinct roles in targeting and promoting GEF activity towards the Rac GTPase, both of which are important for the directed movements of motorneurons in vivo. ..
- Dixon S, Alexander M, Fernandes R, Ricker N, Roy P. FGF negatively regulates muscle membrane extension in Caenorhabditis elegans. Development. 2006;133:1263-75 pubmed..Our data are consistent with a model in which integrins and laminins are needed for directed muscle arm extension to the nerve cords, while FGF signaling provides a general mechanism to regulate muscle membrane extension. ..
- Levy Strumpf N, Culotti J. VAB-8, UNC-73 and MIG-2 regulate axon polarity and cell migration functions of UNC-40 in C. elegans. Nat Neurosci. 2007;10:161-8 pubmed..These data are indicative of previously unidentified regulatory roles for VAB-8 and small GTPases, which act together to regulate guidance receptor functions. ..
- Zhu F, Lawo S, Bird A, Pinchev D, Ralph A, Richter C, et al. The mammalian SPD-2 ortholog Cep192 regulates centrosome biogenesis. Curr Biol. 2008;18:136-41 pubmed publisher..Both proteins are then required for NEDD-1 recruitment and the subsequent assembly of gamma-TuRCs and other factors into fully functional centrosomes. ..
- Yeh E, Ng S, Zhang M, Bouhours M, Wang Y, Wang M, et al. A putative cation channel, NCA-1, and a novel protein, UNC-80, transmit neuronal activity in C. elegans. PLoS Biol. 2008;6:e55 pubmed publisher..We propose that NCA-1 and UNC-80 regulate neuronal activity at least in part by transmitting depolarization signals to synapses in C. elegans neurons. ..
- Bouhours M, Po M, Gao S, Hung W, Li H, Georgiou J, et al. A co-operative regulation of neuronal excitability by UNC-7 innexin and NCA/NALCN leak channel. Mol Brain. 2011;4:16 pubmed publisher..We propose that, in addition to gap junction-mediated functions, UNC-7 innexin may also form hemichannels to regulate C. elegans' neuronal activity cooperatively with the NCA family leak channels...