Experts and Doctors on bacterial proteins in Halifax, Nova Scotia, Canada

Summary

Locale: Halifax, Nova Scotia, Canada
Topic: bacterial proteins

Top Publications

  1. Abachi S, Lee S, Rupasinghe H. Molecular Mechanisms of Inhibition of Streptococcus Species by Phytochemicals. Molecules. 2016;21: pubmed publisher
    ..Collectively, findings from numerous studies suggest that phytochemicals could be used as drugs for elimination of infections with minimal side effects. ..
  2. Toptchieva A, Sisson G, Bryden L, Taylor D, Hoffman P. An inducible tellurite-resistance operon in Proteus mirabilis. Microbiology. 2003;149:1285-95 pubmed
    ..based on screening for high level Te(r) and preliminary PCR analysis. Thus, a chromosomally located inducible tellurite resistance operon appears to be a common feature of the genus Proteus. ..
  3. Faulkner G, Berk S, Garduno E, Ortiz Jimenez M, Garduno R. Passage through Tetrahymena tropicalis triggers a rapid morphological differentiation in Legionella pneumophila. J Bacteriol. 2008;190:7728-38 pubmed publisher
    ..The Tetrahymena model can now be exploited to study the signals that trigger MIF development in vivo and is the only replication-independent model reported to date that allows the differentiation of Dot/Icm mutants into MIFs. ..
  4. Halperin S, Gupta A, Jeanfreau R, Klein N, Reisinger K, Walter E, et al. Comparison of the safety and immunogenicity of an investigational and a licensed quadrivalent meningococcal conjugate vaccine in children 2-10 years of age. Vaccine. 2010;28:7865-72 pubmed publisher
    ..Seroresponse to MenACWY-CRM was statistically noninferior to MCV4 for all groups, and statistically superior for groups C, W, and Y. Clinicaltrials.gov identifier: NCT00616421. ..
  5. Davey L, Cohen A, Leblanc J, Halperin S, Lee S. The disulfide oxidoreductase SdbA is active in Streptococcus gordonii using a single C-terminal cysteine of the CXXC motif. Mol Microbiol. 2016;99:236-53 pubmed publisher
    ..These results provide evidence that certain disulfide oxidoreductases can catalyze disulfide bond formation using a single cysteine of the CXXC motif, including the buried C-terminal cysteine. ..
  6. Davey L, Ng C, Halperin S, Lee S. Functional analysis of paralogous thiol-disulfide oxidoreductases in Streptococcus gordonii. J Biol Chem. 2013;288:16416-29 pubmed publisher
    ..Our results demonstrate that SdbA is required for disulfide bond formation in S. gordonii and indicate that this enzyme may represent a novel type of oxidoreductase in Gram-positive bacteria. ..
  7. Volkmann G, Liu X. Intein lacking conserved C-terminal motif G retains controllable N-cleavage activity. FEBS J. 2011;278:3431-46 pubmed publisher
    ..Our findings reveal interesting insights into intein structure-function relationships, and demonstrate a new and potentially more useful method of controllable, intein-mediated N-cleavage for protein engineering applications. ..
  8. Appleby Tagoe J, Thiel I, Wang Y, Wang Y, Mootz H, Liu X. Highly efficient and more general cis- and trans-splicing inteins through sequential directed evolution. J Biol Chem. 2011;286:34440-7 pubmed publisher
    ..Implications on the intein structure-function, practical application, and evolution are discussed. ..
  9. Melano R, Davidson R, Musgrave H, Forward K. Cephalosporin resistance in Klebsiella pneumoniae from Nova Scotia, Canada. Diagn Microbiol Infect Dis. 2006;56:197-205 pubmed
    ..The combined effects of narrow-spectrum beta-lactamase production and decreased or nonexpression of OmpK35/36 porins did not account for the cefoxitin resistance observed in some of these strains. ..

More Information

Publications49

  1. Dacanay A, Knickle L, Solanky K, Boyd J, Walter J, Brown L, et al. Contribution of the type III secretion system (TTSS) to virulence of Aeromonas salmonicida subsp. salmonicida. Microbiology. 2006;152:1847-56 pubmed publisher
    ..The DeltaascC i.p. injection data also suggest that in addition to host invasion there is a second step in A. salmonicida pathogenesis that requires an active TTSS...
  2. Lee S, Andrian E, Rowland E, Marquez I. Immune response and alveolar bone resorption in a mouse model of Treponema denticola infection. Infect Immun. 2009;77:694-8 pubmed publisher
    ..denticola. Mandibles of infected mice showed significantly greater bone resorption (P < 0.01) than those of mock-infected controls...
  3. Nasrallah G, Riveroll A, Chong A, Murray L, Lewis P, Garduno R. Legionella pneumophila requires polyamines for optimal intracellular growth. J Bacteriol. 2011;193:4346-60 pubmed publisher
    ..pneumophila, further suggesting a need for exogenous polyamines. We hypothesize that HtpB may function to ensure a supply of polyamines in host cells, which are required for the optimal intracellular growth of L. pneumophila...
  4. Bui T, Akay T, Loubani O, Hnasko T, Jessell T, Brownstone R. Circuits for grasping: spinal dI3 interneurons mediate cutaneous control of motor behavior. Neuron. 2013;78:191-204 pubmed publisher
    ..Thus, spinal microcircuits that integrate cutaneous feedback crucial for paw grip rely on the intermediary role of dI3 interneurons. ..
  5. Yang K, Han L, Vining L. Regulation of jadomycin B production in Streptomyces venezuelae ISP5230: involvement of a repressor gene, jadR2. J Bacteriol. 1995;177:6111-7 pubmed
    ..When cultures of the disruption mutants were ethanol stressed, they overproduced the antibiotic. From these results it was concluded that expression of the jadomycin B biosynthesis genes are negatively regulated by jadR2. ..
  6. Ebanks R, Goguen M, Knickle L, Dacanay A, Leslie A, Ross N, et al. Analysis of a ferric uptake regulator (Fur) knockout mutant in Aeromonas salmonicida subsp. salmonicida. Vet Microbiol. 2013;162:831-41 pubmed publisher
    ..In addition, the A. salmonicida fur mutant provides an important tool for further investigation of the iron acquisition mechanisms utilized by A. salmonicida...
  7. Chute I, Hu Z, Liu X. A topA intein in Pyrococcus furiosus and its relatedness to the r-gyr intein of Methanococcus jannaschii. Gene. 1998;210:85-92 pubmed
    ..It is suggested that these two inteins are homologous inteins present in structurally related, but functionally distinct, proteins, with implications on intein evolution and intein homing. ..
  8. Steeves C, Potrykus J, Barnett D, Bearne S. Oxidative stress response in the opportunistic oral pathogen Fusobacterium nucleatum. Proteomics. 2011;11:2027-37 pubmed publisher
    ..e. ClpB, DnaK, HtpG, and HrcA) were increased...
  9. Liu X, Hu Z. A DnaB intein in Rhodothermus marinus: indication of recent intein homing across remotely related organisms. Proc Natl Acad Sci U S A. 1997;94:7851-6 pubmed
    ..marinus DnaB intein coding sequence differs markedly from the codon usages of its flanking extein coding sequences and other genes in the same genome, suggesting more recent acquisition of the DnaB intein in this organism...
  10. Ragan M, Logsdon J, Sensen C, Charlebois R, Doolittle W. An archaebacterial homolog of pelota, a meiotic cell division protein in eukaryotes. FEMS Microbiol Lett. 1996;144:151-5 pubmed
    ..The nuclear localization signal and negatively charged carboxy-terminus characteristic of eukaryotic pelota-like proteins are absent from the S. solfataricus homolog, and hence may be indicative of the acquired eukaryotic function(s)...
  11. Brown J, Masuchi Y, Robb F, Doolittle W. Evolutionary relationships of bacterial and archaeal glutamine synthetase genes. J Mol Evol. 1994;38:566-76 pubmed
    ..The GSI gene of Sulfolobus solfataricus, a member of the Crenarchaeota (extreme thermophiles), is exceptional and could not be definitely placed in either subdivision...
  12. Sharma A, Zhaxybayeva O, Papke R, Doolittle W. Actinorhodopsins: proteorhodopsin-like gene sequences found predominantly in non-marine environments. Environ Microbiol. 2008;10:1039-56 pubmed publisher
  13. Liu X, Yang J. Bacterial thymidylate synthase with intein, group II Intron, and distinctive ThyX motifs. J Bacteriol. 2004;186:6316-9 pubmed
    ..One ThyX sequence has an intein in its ThyX motif that was shown to do protein splicing and a group II intron in its gene, suggesting a hot spot for these self-splicing mobile elements. ..
  14. Salsman J, Dellaire G. Precision genome editing in the CRISPR era. Biochem Cell Biol. 2017;95:187-201 pubmed publisher
  15. Sarty D, Baker N, Thomson E, Rafuse C, Ebanks R, Graham L, et al. Characterization of the type III secretion associated low calcium response genes of Vibrio parahaemolyticus RIMD2210633. Can J Microbiol. 2012;58:1306-15 pubmed publisher
    ..parahaemolyticus-mediated host-cell cytotoxicity during infection. These approaches should be valuable towards future detailed genetic and biochemical analyses of T3SS-1 in V. parahaemolyticus...
  16. Ebanks R, Knickle L, Goguen M, Boyd J, Pinto D, Reith M, et al. Expression of and secretion through the Aeromonas salmonicida type III secretion system. Microbiology. 2006;152:1275-86 pubmed
    ..This study presents a simple in vitro model for the expression of TTSS proteins in A. salmonicida. ..
  17. Allard J, Hill A, Rutenberg A. Heterocyst patterns without patterning proteins in cyanobacterial filaments. Dev Biol. 2007;312:427-34 pubmed
    ..Y., Golden, J.W., 2004. Different functions of HetR, a master regulator of heterocyst differentiation in Anabaena sp PCC 7120, can be separated by mutation. Proc. Natl. Acad. Sci. U. S. A. 101, 16040-16045]. ..
  18. Syvitski R, Tian X, Sampara K, Salman A, Lee S, Jakeman D, et al. Structure-activity analysis of quorum-sensing signaling peptides from Streptococcus mutans. J Bacteriol. 2007;189:1441-50 pubmed
    ..This study is the first study to recognize the importance of the signaling peptide C-terminal residues in streptococcal quorum sensing. ..
  19. Douglas S. A secY homologue is found in the plastid genome of Cryptomonas phi. FEBS Lett. 1992;298:93-6 pubmed
    ..coli homologue. This report of a secY homologue in a plastid genome provides preliminary evidence that a prokaryotic-like protein export system may be operating in plastids. ..
  20. Croxen M, Sisson G, Melano R, Hoffman P. The Helicobacter pylori chemotaxis receptor TlpB (HP0103) is required for pH taxis and for colonization of the gastric mucosa. J Bacteriol. 2006;188:2656-65 pubmed publisher
    ..pH taxis, like motility and urease activity, is essential for colonization and persistence in the gastric mucosa, and thus TlpB function might represent a novel target in the development of therapeutics that blind tactic behavior...
  21. Brown M, Aidoo K, Vining L. A role for pabAB, a p-aminobenzoate synthase gene of Streptomyces venezuelae ISP5230, in chloramphenicol biosynthesis. Microbiology. 1996;142 ( Pt 6):1345-55 pubmed
    ..venezuelae chromosomal pabAB with the disrupted copy lowered sulfanilamide resistance from 25 to 5 micrograms mL-1 and blocked Cm production. ..
  22. Li A, Too C, Knee R, Wilkinson M, Murphy P. FGF-2 antisense RNA encodes a nuclear protein with MutT-like antimutator activity. Mol Cell Endocrinol. 1997;133:177-82 pubmed
    ..These findings demonstrate for the first time that the FGF-AS transcript encodes a functional nuclear protein with MutT-related enzymatic activity. ..
  23. Boyd J, Williams J, Curtis B, Kozera C, Singh R, Reith M. Three small, cryptic plasmids from Aeromonas salmonicida subsp. salmonicida A449. Plasmid. 2003;50:131-44 pubmed
    ..A survey of A. salmonicida strains indicates that pAsa1 and pAsa2 are present in all 12 strains investigated, while pAsa3 is present in 11 and a fourth plasmid, pAsal1, is present in 7...
  24. Dacanay A, Johnson S, Bjornsdottir R, Ebanks R, Ross N, Reith M, et al. Molecular characterization and quantitative analysis of superoxide dismutases in virulent and avirulent strains of Aeromonas salmonicida subsp. salmonicida. J Bacteriol. 2003;185:4336-44 pubmed
    ..Levels of SOD were significantly higher in virulent than in avirulent strains of A. salmonicida subsp. salmonicida strain A449 when cultured in vitro and in vivo. ..
  25. Garduno R, Moore A, Olivier G, Lizama A, Garduno E, Kay W. Host cell invasion and intracellular residence by Aeromonas salmonicida: role of the S-layer. Can J Microbiol. 2000;46:660-8 pubmed
    ..These cell lines should prove useful in studies aimed at characterizing the invasion mechanisms of A. salmonicida, but of limited value in studying the intracellular residence and replication of this invasive bacterium in vitro. ..
  26. He J, Magarvey N, Piraee M, Vining L. The gene cluster for chloramphenicol biosynthesis in Streptomyces venezuelae ISP5230 includes novel shikimate pathway homologues and a monomodular non-ribosomal peptide synthetase gene. Microbiology. 2001;147:2817-29 pubmed
    ..As has been shown for ORF2, disrupting ORF3, ORF7, ORF8 or ORF9 blocked Cm production. ..
  27. Dacks J, Doolittle W. Reconstructing/deconstructing the earliest eukaryotes: how comparative genomics can help. Cell. 2001;107:419-25 pubmed
    ..It's not clear that either is the case, but the expanding protist genomic database could help us in several ways...
  28. Keating M, Gong H, Byers D. Identification of a key residue in the conformational stability of acyl carrier protein. Biochim Biophys Acta. 2002;1601:208-14 pubmed
    ..Together, these results suggest that the partial positive charge of His-75 may be important in maintaining the conformational stability of E. coli ACP at a neutral pH. ..
  29. Brassinga A, Siam R, McSween W, Winkler H, Wood D, Marczynski G. Conserved response regulator CtrA and IHF binding sites in the alpha-proteobacteria Caulobacter crescentus and Rickettsia prowazekii chromosomal replication origins. J Bacteriol. 2002;184:5789-99 pubmed
    ..Therefore, CtrA and IHF regulatory proteins have similar binding patterns in both replication origins, and we propose that CzcR is a global cell cycle regulator in R. prowazekii...
  30. Croxen M, Ernst P, Hoffman P. Antisense RNA modulation of alkyl hydroperoxide reductase levels in Helicobacter pylori correlates with organic peroxide toxicity but not infectivity. J Bacteriol. 2007;189:3359-68 pubmed publisher
    ..These studies also demonstrate the utility of antisense RNA interference in the evaluation of gene function in H. pylori...
  31. Wang S, Liu X. The plastid genome of Cryptomonas phi encodes an hsp70-like protein, a histone-like protein, and an acyl carrier protein. Proc Natl Acad Sci U S A. 1991;88:10783-7 pubmed
    ..Its predicted amino acid sequence is 36-59% identical to that of eubacterial and plant chloroplast (nucleus-encoded) acyl carrier proteins...
  32. Smithen M, Elustondo P, Winkfein R, Zakharian E, Abramov A, Pavlov E. Role of polyhydroxybutyrate in mitochondrial calcium uptake. Cell Calcium. 2013;54:86-94 pubmed publisher
    ..We propose that PHB is a previously unrecognized important component of the mitochondrial calcium uptake system...
  33. Andersson J, Roger A. Evolution of glutamate dehydrogenase genes: evidence for lateral gene transfer within and between prokaryotes and eukaryotes. BMC Evol Biol. 2003;3:14 pubmed
  34. Morash M, Brassinga A, Warthan M, Gourabathini P, Garduno R, Goodman S, et al. Reciprocal expression of integration host factor and HU in the developmental cycle and infectivity of Legionella pneumophila. Appl Environ Microbiol. 2009;75:1826-37 pubmed publisher
    ..These studies establish IHF and HU as markers of developmental stages and show that IHF function is required for both differentiation and full virulence of L. pneumophila in natural amoebic hosts. ..
  35. Potrykus J, Mahaney B, White R, Bearne S. Proteomic investigation of glucose metabolism in the butyrate-producing gut anaerobe Fusobacterium varium. Proteomics. 2007;7:1839-53 pubmed
    ..varium...
  36. Lee S, Boran T. Roles of sortase in surface expression of the major protein adhesin P1, saliva-induced aggregation and adherence, and cariogenicity of Streptococcus mutans. Infect Immun. 2003;71:676-81 pubmed
    ..05) than the wild-type strain. In conclusion, the sortase enzyme in S. mutans is responsible for anchoring P1 to the cell surface and plays a role in modulating the surface properties and cariogenicity of S. mutans...
  37. Vats N, Lee S. Characterization of a copper-transport operon, copYAZ, from Streptococcus mutans. Microbiology. 2001;147:653-62 pubmed publisher
    ..By using a chloramphenicol acetyltransferase reporter gene fusion, the cop operon was shown to be negatively regulated by CopY and could be derepressed by Cu2+...
  38. Stairs C, Roger A, Hampl V. Eukaryotic pyruvate formate lyase and its activating enzyme were acquired laterally from a Firmicute. Mol Biol Evol. 2011;28:2087-99 pubmed publisher
  39. Nesb C, Doolittle W. Targeting clusters of transferred genes in Thermotoga maritima. Environ Microbiol. 2003;5:1144-54 pubmed
  40. Lee S, Delaney G, Elkhateeb M. A two-component covRS regulatory system regulates expression of fructosyltransferase and a novel extracellular carbohydrate in Streptococcus mutans. Infect Immun. 2004;72:3968-73 pubmed publisher
    ..In conclusion, the results indicate that the expression of FTF and a glucose- and glucuronic acid-containing carbohydrate was negatively regulated by the covRS two-component regulatory system in S. mutans...