Genomes and Genes
Experts and Doctors on cultured cells in Ontario, Canada
Locale: Ontario, Canada
Topic: cultured cells
- Heng H, Xie B, Shi X, Tsui L, Mahuran D. Refined mapping of the GM2 activator protein (GM2A) locus to 5q31.3-q33.1, distal to the spinal muscular atrophy locus. Genomics. 1993;18:429-31 pubmed..In this report, we reconsider the candidacy of GM2A by refining its localization on chromosome 5 using fluorescence in situ hybridization. We localize GM2A to 5q31.3-q33.1; thus, it is not a candidate gene for SMA. ..
- Yogi A, Callera G, Hipólito U, Silva C, Touyz R, Tirapelli C. Ethanol-induced vasoconstriction is mediated via redox-sensitive cyclo-oxygenase-dependent mechanisms. Clin Sci (Lond). 2010;118:657-68 pubmed publisher..These findings identify putative molecular mechanisms whereby ethanol, at high concentrations, influences vascular reactivity. Whether similar phenomena occur in vivo at lower concentrations of ethanol remains unclear. ..
- Zhang L, Rodriguez Del Castillo A, Trifaro J. Histamine-evoked chromaffin cell scinderin redistribution, F-actin disassembly, and secretion: in the absence of cortical F-actin disassembly, an increase in intracellular Ca2+ fails to trigger exocytosis. J Neurochem. 1995;65:1297-308 pubmed..The results also show that in the absence of F-actin disassembly, rises in intracellular Ca2+ concentration are not by themselves capable of triggering catecholamine release. ..
- Bellows C, Aubin J, Heersche J. Initiation and progression of mineralization of bone nodules formed in vitro: the role of alkaline phosphatase and organic phosphate. Bone Miner. 1991;14:27-40 pubmed
- Wei Q, Croy B, Etches R. Selection of genetically modified chicken blastodermal cells by magnetic-activated cell sorting. Poult Sci. 2001;80:1671-8 pubmed
- Karakji E, Tsang B. Follicular stage-dependent regulation of rat granulosa cell plasminogen activator system by transforming growth factor-alpha in vitro. Biol Reprod. 1995;52:411-8 pubmed..abstract truncated at 250 words) ..
- Wang M, Drucker D. The LIM domain homeobox gene isl-1: conservation of human, hamster, and rat complementary deoxyribonucleic acid sequences and expression in cell types of nonneuroendocrine lineage. Endocrinology. 1994;134:1416-22 pubmed..These experiments suggest that the biological importance of isl-1 may not be restricted to neuroendocrine cell lineages and raise the possibility that isl-1 function may be regulated by sequestration in distinct cellular compartments. ..
- Zobel C, Kassiri Z, Nguyen T, Meng Y, Backx P. Prevention of hypertrophy by overexpression of Kv4.2 in cultured neonatal cardiomyocytes. Circulation. 2002;106:2385-91 pubmed..The clinical implications of these studies and the possible involvement of other signaling pathways are discussed. ..
- Catz Biro L, Chin W, Archer M, Pollanen M, Hayes M. Toxicity of N-nitrosodimethylamine, N-nitrosomethylbenzylamine, and 1,2-dimethylhydrazine in isolated rat hepatocytes. Toxicol Appl Pharmacol. 1990;102:191-4 pubmed
- Verma S, Badiwala M, Weisel R, Li S, Wang C, Fedak P, et al. C-reactive protein activates the nuclear factor-kappaB signal transduction pathway in saphenous vein endothelial cells: implications for atherosclerosis and restenosis. J Thorac Cardiovasc Surg. 2003;126:1886-91 pubmed..These data have important implications for saphenous vein atherosclerosis in patients with elevated C-reactive protein levels. ..
- Jia D, Heersche J. Pregnancy-associated plasma protein-A proteolytic activity in rat vertebral cell cultures: stimulation by dexamethasone--a potential mechanism for glucocorticoid regulation of osteoprogenitor proliferation and differentiation. J Cell Physiol. 2005;204:848-58 pubmed..Our findings underscore a novel mechanism whereby GCs increase IGF responses in rat bone cells via PAPP-A-induced IGFBP-4 proteolysis. ..
- Fisher C, Parks R, Lauzon M, Evans D. Heteroduplex DNA formation is associated with replication and recombination in poxvirus-infected cells. Genetics. 1991;129:7-18 pubmed..The significance of these observations are discussed considering some of the unique properties of poxviral biology...
- Callera G, He Y, Yogi A, Montezano A, Paravicini T, Yao G, et al. Regulation of the novel Mg2+ transporter transient receptor potential melastatin 7 (TRPM7) cation channel by bradykinin in vascular smooth muscle cells. J Hypertens. 2009;27:155-66 pubmed..These data identify TRPM7/annexin-1/Mg2+ as a novel pathway in bradykinin signaling. ..
- Niknahad H, Khan S, Sood C, O Brien P. Prevention of cyanide-induced cytotoxicity by nutrients in isolated rat hepatocytes. Toxicol Appl Pharmacol. 1994;128:271-9 pubmed..Furthermore, all glycogenic and gluconeogenic amino acids and carbohydrates were cytoprotective against cyanide toxicity toward fasted hepatocytes, suggesting that cellular energy stores determine their resistance to cyanide. ..
- Liu F, Wan Q, Pristupa Z, Yu X, Wang Y, Niznik H. Direct protein-protein coupling enables cross-talk between dopamine D5 and gamma-aminobutyric acid A receptors. Nature. 2000;403:274-80 pubmed
- Yu Q, Yue F, Gu X, Schwartz H, Kovacs C, Ostrowski M. OX40 ligation of CD4+ T cells enhances virus-specific CD8+ T cell memory responses independently of IL-2 and CD4+ T regulatory cell inhibition. J Immunol. 2006;176:2486-95 pubmed..Thus, OX40 ligation on CD4(+) T cells represents a potentially novel immunotherapeutic strategy that should be investigated to treat and prevent persistent virus infections, such as HIV-1 infection. ..
- Skalski V, Brown K, Choi B, Lin Z, Chen S. A 3'-5' exonuclease in human leukemia cells: implications for resistance to 1-beta -D-arabinofuranosylcytosine and 9-beta -D-arabinofuranosyl-2-fluoroadenine 5'-monophosphate. J Biol Chem. 2000;275:25814-9 pubmed..Its physical and enzymatic properties indicate that exoN is a previously uncharacterized enzyme whose activity may confer resistance to clinical nucleoside analogs in leukemia cells. ..
- Li C, Nguyen Q, Cole W, Alman B. Potential treatment for clubfeet based on growth factor blockade. J Pediatr Orthop. 2001;21:372-7 pubmed..Growth factor blockade has the potential to change the behavior of these cells in a way that would lessen the severity of the contractures, perhaps improving the outcome of clubfoot treatment. ..
- Barbara N, Wrana J, Letarte M. Endoglin is an accessory protein that interacts with the signaling receptor complex of multiple members of the transforming growth factor-beta superfamily. J Biol Chem. 1999;274:584-94 pubmed..Our data suggest that endoglin is an accessory protein of multiple kinase receptor complexes of the TGF-beta superfamily. ..
- Bani Yaghoub M, Kubu C, Cowling R, Rochira J, Nikopoulos G, Bellum S, et al. A switch in numb isoforms is a critical step in cortical development. Dev Dyn. 2007;236:696-705 pubmed..This finding suggests that numb may have other signaling properties during neuronal differentiation in addition to augmenting notch signal strength. ..
- Carnegie J, Byard R, Dardick I, Tsang B. Culture of granulosa cells in collagen gels: the influence of cell shape on steroidogenesis. Biol Reprod. 1988;38:881-90 pubmed
- Bunda S, Liu P, Wang Y, Liu K, Hinek A. Aldosterone induces elastin production in cardiac fibroblasts through activation of insulin-like growth factor-I receptors in a mineralocorticoid receptor-independent manner. Am J Pathol. 2007;171:809-19 pubmed
- Heximer S. RGS2-mediated regulation of Gqalpha. Methods Enzymol. 2004;390:65-82 pubmed..Results from these studies have been used to demonstrate the high relative potency of RGS2 for the regulation of Gq signaling at the biochemical, cellular, and physiologic level. ..
- Inman R, Payne U. Determinants of synoviocyte clearance of arthritogenic bacteria. J Rheumatol. 2003;30:1291-7 pubmed..Characterizing these mechanisms holds the promise of more specific therapeutic interventions in this disease. ..
- Michaud J, Hosseini Abardeh M, Farah K, Kennedy C. Modulating alpha-actinin-4 dynamics in podocytes. Cell Motil Cytoskeleton. 2009;66:166-78 pubmed publisher..These data suggest that dynamic regulation of alpha-actinin-4/actin interactions may be necessary for maintaining podocyte structure in response to glomerular hydrostatic forces. ..
- Bellows C, Ciaccia A, Heersche J. Osteoprogenitor cells in cell populations derived from mouse and rat calvaria differ in their response to corticosterone, cortisol, and cortisone. Bone. 1998;23:119-25 pubmed..In conclusion, our results show that the glucocorticoids corticosterone and cortisol inhibit proliferation and differentiation of osteoprogenitors in MC cell cultures but stimulate these processes in rat-derived osteoprogenitors. ..
- Lefebvre D, Bai Y, Shahmolky N, Sharma M, Poon R, Drucker D, et al. Identification and characterization of a novel sucrose-non-fermenting protein kinase/AMP-activated protein kinase-related protein kinase, SNARK. Biochem J. 2001;355:297-305 pubmed..These findings identify SNARK as a glucose- and AICAriboside-regulated member of the AMPK-related gene family that represents a new candidate mediator of the cellular response to metabolic stress. ..
- Pollanen M, Quinn B, Wollenberg G, Hayes M. Reversible mitochondrial swelling in cultured rat hepatocytes exposed to 1,2-dimethylhydrazine. Exp Mol Pathol. 1990;52:170-8 pubmed
- Rodriguez Del Castillo A, Vitale M, Trifaro J. Ca2+ and pH determine the interaction of chromaffin cell scinderin with phosphatidylserine and phosphatidylinositol 4,5,-biphosphate and its cellular distribution during nicotinic-receptor stimulation and protein kinase C activation. J Cell Biol. 1992;119:797-810 pubmed..In view of these findings, a model which can explain how scinderin redistribution and activity may be regulated by pH and Ca2+ in resting and stimulated conditions is proposed. ..
- Joo D, Gong D, Sonner J, Jia Z, MacDonald J, Eger E, et al. Blockade of AMPA receptors and volatile anesthetics: reduced anesthetic requirements in GluR2 null mutant mice for loss of the righting reflex and antinociception but not minimum alveolar concentration. Anesthesiology. 2001;94:478-88 pubmed..GluR2-containing receptors do not contribute appreciably to MAC in this mouse model. These results illustrate the difficulties in attributing behavioral responses to drug-receptor interactions in genetically engineered animals. ..
- Nguyen L, Duncan G, Mirtsos C, Ng M, Speiser D, Shahinian A, et al. TRAF2 deficiency results in hyperactivity of certain TNFR1 signals and impairment of CD40-mediated responses. Immunity. 1999;11:379-89 pubmed..These data demonstrate two important roles of TRAF2, one as a negative regulator of certain TNFR1 signals and the other as a positive mediator of CD40 signaling. ..
- Chun K, Mackay N, Petrova Benedict R, Robinson B. Pyruvate dehydrogenase deficiency due to a 20-bp deletion in exon II of the pyruvate dehydrogenase (PDH) E1 alpha gene. Am J Hum Genet. 1991;49:414-20 pubmed..The 20-bp deletion was not found in either parent's genomic DNA, and hence we conclude that the mutation must have occurred de novo, either in the germ-line cells or immediately following fertilization. ..
- Rodriguez Del Castillo A, Lemaire S, Tchakarov L, Jeyapragasan M, Doucet J, Vitale M, et al. Chromaffin cell scinderin, a novel calcium-dependent actin filament-severing protein. EMBO J. 1990;9:43-52 pubmed..Alternatively, the actin filament-severing activity of the two proteins might be under the control of different transduction and modulating influences. ..
- Carlyle J, Michie A, Cho S, Zúñiga Pflücker J. Natural killer cell development and function precede alpha beta T cell differentiation in mouse fetal thymic ontogeny. J Immunol. 1998;160:744-53 pubmed..These results provide the first evidence of functional NK lymphocytes in mouse fetal ontogeny and demonstrate that NK cell maturation precedes alpha beta T cell development in the fetal thymus. ..
- Choi J, Chiang A, Taulier N, Gros R, Pirani A, Husain M. A calmodulin-binding site on cyclin E mediates Ca2+-sensitive G1/s transitions in vascular smooth muscle cells. Circ Res. 2006;98:1273-81 pubmed..Taken together, these findings reveal CaM-dependent cyclin E/CDK2 activity as a mediator of the known Ca2+ sensitivity of the G1/S transition of VSMC. ..
- Hoodless P, Haerry T, Abdollah S, Stapleton M, O Connor M, Attisano L, et al. MADR1, a MAD-related protein that functions in BMP2 signaling pathways. Cell. 1996;85:489-500 pubmed..BMP2 treatment results in accumulation of MADR1 in the nucleus. MAD proteins may thus define a novel class of signaling molecules with nuclear function in Ser/Thr kinase receptor signaling pathways. ..
- Parks R, Evans D. Enhanced recombination associated with the presence of insertion and deletion mutations in poxvirus-infected cells. Virology. 1991;184:299-309 pubmed..The similarities between these observations and data derived from phage and fungal experiments also provide fundamental insights into the mechanism of poxviral recombination...
- Conway E, Liu L, Nowakowski B, Steiner Mosonyi M, Ribeiro S, Michalak M. Heat shock-sensitive expression of calreticulin. In vitro and in vivo up-regulation. J Biol Chem. 1995;270:17011-6 pubmed
- Misener V, Hui C, Malapitan I, Ittel M, Joyner A, Jongstra J. Expression of mouse LSP1/S37 isoforms. S37 is expressed in embryonic mesenchymal cells. J Cell Sci. 1994;107 ( Pt 12):3591-600 pubmed..This, together with our finding that S37 is an F-actin binding protein, suggests that S37 is a cytoskeletal protein of mesenchymal cells, which may play a role in mesenchyme-induced epithelial differentiation during organogenesis. ..
- Hinek A, Pshezhetsky A, von Itzstein M, Starcher B. Lysosomal sialidase (neuraminidase-1) is targeted to the cell surface in a multiprotein complex that facilitates elastic fiber assembly. J Biol Chem. 2006;281:3698-710 pubmed..In turn, the exposed galactosugars interact with the galectin domain of EBP, thereby inducing the release of transported tropoelastin molecules and facilitating their subsequent assembly into elastic fibers. ..
- Adiguzel E, Hou G, Mulholland D, Hopfer U, Fukai N, Olsen B, et al. Migration and growth are attenuated in vascular smooth muscle cells with type VIII collagen-null alleles. Arterioscler Thromb Vasc Biol. 2006;26:56-61 pubmed..This study suggests that SMCs can modify the matrix microenvironment by producing type VIII collagen, using it to overlay type I collagen, and generating a substrate favorable for migration. ..
- Taylor S, Jodouin C, Richter M. Cells and mediators which participate in immunoglobulin synthesis by human mononuclear cells. III. Null cells secrete a factor(s) (human immunoglobulin synthesis/secretion-facilitating factor) that can replace the null cells in the synthesis of immun. Clin Exp Immunol. 1990;80:130-5 pubmed
- Fong B, Watson P, Watson A. Mouse preimplantation embryo responses to culture medium osmolarity include increased expression of CCM2 and p38 MAPK activation. BMC Dev Biol. 2007;7:2 pubmed..These results define the expression of upstream activators of p38 MAPK during preimplantation development and indicate that embryo responses to hyperosmotic environments include elevation of CCM2 and activation of p38 MAPK. ..
- Falconi D, Aubin J. LIF inhibits osteoblast differentiation at least in part by regulation of HAS2 and its product hyaluronan. J Bone Miner Res. 2007;22:1289-300 pubmed..Together the data suggest that osteoblast differentiation is acutely sensitive to HA levels and that LIF inhibits osteoblast development at least in part by stimulating high molecular weight HA synthesis through HAS2. ..
- Du M, Perry R, Nowacki N, Gordon J, Salma J, Zhao J, et al. Protein kinase A represses skeletal myogenesis by targeting myocyte enhancer factor 2D. Mol Cell Biol. 2008;28:2952-70 pubmed publisher..Collectively, these data identify MEF2D as a primary target of PKA signaling in myoblasts that leads to inhibition of the skeletal muscle differentiation program. ..
- Majumdar P, Chen S, George B, Sen S, Karmazyn M, Chakrabarti S. Leptin and endothelin-1 mediated increased extracellular matrix protein production and cardiomyocyte hypertrophy in diabetic heart disease. Diabetes Metab Res Rev. 2009;25:452-63 pubmed publisher..Hearts from the diabetic animals showed hypertrophy and similar mRNA changes. These data indicate that in diabetes increased FN production and cardiomyocyte hypertrophy may be mediated through leptin with its interaction with ET-1. ..
- Diao J, Winter E, Chen W, Cantin C, Cattral M. Characterization of distinct conventional and plasmacytoid dendritic cell-committed precursors in murine bone marrow. J Immunol. 2004;173:1826-33 pubmed..Both DC precursor populations can give rise to CD8 alpha(+) and CD8 alpha(-) DC subtypes. Our findings indicate that CD11c(+)MHC II(-)B220(+) and CD11c(+)MHC II(-)B220(-) bone marrow cells are distinct DC lineage-restricted precursors...
- Hervé J, Crump D, Jones S, Mundy L, Giesy J, Zwiernik M, et al. Cytochrome P4501A induction by 2,3,7,8-tetrachlorodibenzo-p-dioxin and two chlorinated dibenzofurans in primary hepatocyte cultures of three avian species. Toxicol Sci. 2010;113:380-91 pubmed publisher..This study indicates that PeCDF is more potent than TCDD in ring-necked pheasant and Japanese quail hepatocytes and that ring-necked pheasant, Japanese quail, and chicken hepatocytes are equally sensitive to CYP1A induction by PeCDF. ..
- Fitzpatrick V, Percival Smith A, Ingles C, Krause H. Homeodomain-independent activity of the fushi tarazu polypeptide in Drosophila embryos. Nature. 1992;356:610-2 pubmed..Here we show that the same phenotype is induced by ectopic expression of a ftz polypeptide containing a deletion in the homeodomain. Thus, ftz can alter gene expression without binding directly to DNA. ..
- Patel F, Clifton V, Chwalisz K, Challis J. Steroid regulation of prostaglandin dehydrogenase activity and expression in human term placenta and chorio-decidua in relation to labor. J Clin Endocrinol Metab. 1999;84:291-9 pubmed..We conclude that cortisol inhibits PGDH activity and expression and that progestagens increase PGDH activity in human chorion and placenta. ..
- Shillabeer G, Kumar V, Tibbo E, Lau D. Arachidonic acid metabolites of the lipoxygenase as well as the cyclooxygenase pathway may be involved in regulating preadipocyte differentiation. Metabolism. 1998;47:461-6 pubmed..03). We concluded that preadipocyte differentiation within adipose tissue may be subject to both positive and negative regulators derived from AA metabolism resulting from both LOX and cyclooxygenase (COX) activity. ..
- Richter M, Kamran M, Jodouin C. Surface receptors and immune activity of purified human circulating mononuclear cells. V. Circulating null cells and a factor secreted by the null cells inhibit the synthesis of receptors for Fc mu by T cells in culture. Eur J Immunol. 1990;20:803-7 pubmed
- Mittrucker H, Shahinian A, Bouchard D, Kundig T, Mak T. Induction of unresponsiveness and impaired T cell expansion by staphylococcal enterotoxin B in CD28-deficient mice. J Exp Med. 1996;183:2481-8 pubmed..Our results indicate that CD28 costimulation is crucial for the T cell-mediated toxicity of SEB and demonstrate that T cell stimulation in the absence of CD28 costimulation induces unresponsiveness in vivo. ..
- Trifaro J, Tang R, Novas M. Monolayer co-culture of rat heart cells and bovine adrenal chromaffin paraneurons. In Vitro Cell Dev Biol. 1990;26:335-47 pubmed
- Ng G, George S, Zastawny R, Caron M, Bouvier M, Dennis M, et al. Human serotonin1B receptor expression in Sf9 cells: phosphorylation, palmitoylation, and adenylyl cyclase inhibition. Biochemistry. 1993;32:11727-33 pubmed..abstract truncated at 250 words) ..
- Waterhouse P, Penninger J, Timms E, Wakeham A, Shahinian A, Lee K, et al. Lymphoproliferative disorders with early lethality in mice deficient in Ctla-4. Science. 1995;270:985-8 pubmed..Thus, CTLA-4 acts as a negative regulator of T cell activation and is vital for the control of lymphocyte homeostasis. ..
- Wu Y, Nadler M, Brennan L, Gish G, Timms J, Fusaki N, et al. The B-cell transmembrane protein CD72 binds to and is an in vivo substrate of the protein tyrosine phosphatase SHP-1. Curr Biol. 1998;8:1009-17 pubmed..By dephosphorylation CD72. SHP-1 may have a positive role in B-cell signaling. These results have potentially important implications for the involvement of CD72 and SHP-1 in B-cell development and autoimmunity. ..
- Wang P, Liu T, Li Z, Ma X, Jin T. Redundant and synergistic effect of Cdx-2 and Brn-4 on regulating proglucagon gene expression. Endocrinology. 2006;147:1950-8 pubmed publisher..Our observations suggest that Cdx-2 and Brn-4, two HD proteins that belong to two different families, exert a synergistic and redundant effect on proglucagon gene expression...
- Lau A, Ramanathan S, Poussier P. Excessive production of nitric oxide by macrophages from DP-BB rats is secondary to the T-lymphopenic state of these animals. Diabetes. 1998;47:197-205 pubmed..Taken together, these results demonstrate that the elevated production of NO by DP-BB macrophages results from the lyp mutation and represents a crucial mechanism through which T-lymphopenia contributes to the development of diabetes. ..
- Wang M, Franklin V, Marcel Y. In vivo reverse cholesterol transport from macrophages lacking ABCA1 expression is impaired. Arterioscler Thromb Vasc Biol. 2007;27:1837-42 pubmed..However, it is yet to be established that the ABCA1 effect is related to specific changes in RCT from the macrophages in vivo...
- Qiu W, Federico L, Naples M, Avramoglu R, Meshkani R, Zhang J, et al. Phosphatase and tensin homolog (PTEN) regulates hepatic lipogenesis, microsomal triglyceride transfer protein, and the secretion of apolipoprotein B-containing lipoproteins. Hepatology. 2008;48:1799-809 pubmed publisher..On loss of PTEN activity, increased lipid substrate availability in the face of reduced hepatic lipoprotein production capacity can rapidly lead to hepatosteatosis and fatty liver. ..
- Heng H, Xiao H, Shi X, Greenblatt J, Tsui L. Genes encoding general initiation factors for RNA polymerase II transcription are dispersed in the human genome. Hum Mol Genet. 1994;3:61-4 pubmed..3 and 11p14-15.1, respectively. This dispersed localization of a group of functionally related gene provides insights into the molecular mechanism of human genome evolution and their possible involvement in human diseases. ..
- Javadov S, Rajapurohitam V, Kilic A, Zeidan A, Choi A, Karmazyn M. Anti-hypertrophic effect of NHE-1 inhibition involves GSK-3beta-dependent attenuation of mitochondrial dysfunction. J Mol Cell Cardiol. 2009;46:998-1007 pubmed publisher..In conclusion, anti-hypertrophic effect of NHE-1 inhibition can be mediated through activation of GSK-3beta which in turn induces inhibition of mPTP opening due to VDAC phosphorylation. ..
- McClellan K, Vanderluit J, Julian L, Andrusiak M, Dugal Tessier D, Park D, et al. The p107/E2F pathway regulates fibroblast growth factor 2 responsiveness in neural precursor cells. Mol Cell Biol. 2009;29:4701-13 pubmed publisher..By identifying novel roles for p107/E2F in regulating genes outside of the classical cell cycle machinery targets, we uncover a new mechanism whereby Rb/E2F mediates proliferation through regulating growth factor responsiveness. ..
- Toyofuku T, Curotto Kurzydlowski K, Narayanan N, MacLennan D. Identification of Ser38 as the site in cardiac sarcoplasmic reticulum Ca(2+)-ATPase that is phosphorylated by Ca2+/calmodulin-dependent protein kinase. J Biol Chem. 1994;269:26492-6 pubmed..Thus phosphorylation of Ser38 in SERCA2 results in a unique activation of Vmax for Ca2+ transport, providing a potential regulatory mechanism for Ca2+ removal from cardiac and other tissues in which SERCA2 is expressed. ..
- Fournier L, Bégin Heick N, Whitfield J, Schwartz J. Comparison of the properties of the ATP-sensitive K+ channels of pancreatic beta-cells of lean and obese (ob/ob) C57BL/6J mice. J Membr Biol. 1992;129:267-76 pubmed..It is concluded that the depolarized state and the hypersecretory response of obese mouse beta-cells are not related to an altered behavior of their ATP-sensitive potassium channels. ..
- Schock S, Jolin Dahel K, Schock P, Staines W, Garcia Munoz M, Arbuthnott G. Striatal interneurons in dissociated cell culture. Histochem Cell Biol. 2010;134:1-12 pubmed publisher..Furthermore, these results reveal a procedure for parallel culture of striatum and cholinergic depleted striatum that can be used to examine the function of the cholinergic interneuron in striatal networks. ..
- Marcu M, Rodriguez Del Castillo A, Vitale M, Trifaro J. Molecular cloning and functional expression of chromaffin cell scinderin indicates that it belongs to the family of Ca(2+)-dependent F-actin severing proteins. Mol Cell Biochem. 1994;141:153-65 pubmed..These data indicate that scinderin is a novel member of the family of Ca(2+)-dependent F-actin severing proteins which includes gelsolin and villin. ..
- D Addario M, Arora P, Ellen R, McCulloch C. Interaction of p38 and Sp1 in a mechanical force-induced, beta 1 integrin-mediated transcriptional circuit that regulates the actin-binding protein filamin-A. J Biol Chem. 2002;277:47541-50 pubmed
- Smith T, Serghides L, Patel S, Febbraio M, Silverstein R, Kain K. CD36-mediated nonopsonic phagocytosis of erythrocytes infected with stage I and IIA gametocytes of Plasmodium falciparum. Infect Immun. 2003;71:393-400 pubmed
- McGilvray I, Tsai V, Marshall J, Dackiw A, Rotstein O. Monocyte adhesion and transmigration induce tissue factor expression: role of the mitogen-activated protein kinases. Shock. 2002;18:51-7 pubmed..The selective inhibition of the mitogen-activated protein kinases may offer a novel therapeutic means of modulating this inflammatory sequence. ..
- Kong Y, Yoshida H, Sarosi I, Tan H, Timms E, Capparelli C, et al. OPGL is a key regulator of osteoclastogenesis, lymphocyte development and lymph-node organogenesis. Nature. 1999;397:315-23 pubmed..Thus OPGL is a new regulator of lymph-node organogenesis and lymphocyte development and is an essential osteoclast differentiation factor in vivo. ..
- Wang J, Shao L, Sun X, Young L. Glutathione S-transferase is a novel target for mood stabilizing drugs in primary cultured neurons. J Neurochem. 2004;88:1477-84 pubmed..The present findings suggest that regulation of GST M1, and possibly GST A4, may mediate the anti-oxidative effects of valproate treatment, and regulation of GST may be involved in the mood stabilizing effect of valproate and lithium. ..
- Ezzat S, Yu S, Asa S. The zinc finger Ikaros transcription factor regulates pituitary growth hormone and prolactin gene expression through distinct effects on chromatin accessibility. Mol Endocrinol. 2005;19:1004-11 pubmed..These data provide evidence for Ikaros-mediated histone acetylation and chromatin remodeling in the selective regulation of pituitary GH and PRL hormone gene expression. ..
- O Hare M, Hou S, Morris E, Cregan S, Xu Q, Slack R, et al. Induction and modulation of cerebellar granule neuron death by E2F-1. J Biol Chem. 2000;275:25358-64 pubmed..These results indicate that E2F-1 expression is sufficient to promote neuronal apoptosis and that endogenous E2F-1 modulates the death of CGNs evoked by low K(+). ..
- Cregan S, Fortin A, Maclaurin J, Callaghan S, Cecconi F, Yu S, et al. Apoptosis-inducing factor is involved in the regulation of caspase-independent neuronal cell death. J Cell Biol. 2002;158:507-17 pubmed..Taken together, our results suggest that AIF may be an important therapeutic target for the treatment of neuronal injury. ..
- Anini Y, Brubaker P. Role of leptin in the regulation of glucagon-like peptide-1 secretion. Diabetes. 2003;52:252-9 pubmed..05). These results show for the first time that leptin stimulates GLP-1 secretion from rodent and human intestinal L cells, and they suggest that leptin resistance may account for the decreased levels of GLP-1 found in obese humans. ..
- Dallas S, Zhu X, Baruchel S, Schlichter L, Bendayan R. Functional expression of the multidrug resistance protein 1 in microglia. J Pharmacol Exp Ther. 2003;307:282-90 pubmed..They also suggest that brain parenchyma can act as a "second" barrier to drug permeability and regulate brain distribution/accumulation of various xenobiotics, including protease inhibitors. ..
- MacLeod D, Gyles C. Purification and characterization of an Escherichia coli Shiga-like toxin II variant. Infect Immun. 1990;58:1232-9 pubmed..Antiserum to the Shiga-like toxin II variant neutralized homologous toxin, Shiga-like toxin II, and Verotoxin 2 but not Shiga-like toxin I...
- Ciruna B, Rossant J. FGF signaling regulates mesoderm cell fate specification and morphogenetic movement at the primitive streak. Dev Cell. 2001;1:37-49 pubmed..We propose that modulation of cytoplasmic beta-catenin levels, associated with FGF-induced downregulation of E-cadherin, provides a molecular link between FGF and Wnt signaling pathways at the streak. ..
- Dumitrescu Pene T, Rosé S, Lejen T, Marcu M, Trifaró J. Expression of various scinderin domains in chromaffin cells indicates that this protein acts as a molecular switch in the control of actin filament dynamics and exocytosis. J Neurochem. 2005;92:780-9 pubmed..Thus, increase in [Ca2+]i produced by stimulation-induced Ca2+ entry would increase Sc-evoked cortical F-actin disassembly. Decrease in [Ca2+]i by either organelle sequestration or cell extrusion would favor Sc-evoked actin nucleation. ..
- Levesque G, Yu G, Nishimura M, Zhang D, Levesque L, Yu H, et al. Presenilins interact with armadillo proteins including neural-specific plakophilin-related protein and beta-catenin. J Neurochem. 1999;72:999-1008 pubmed..The latter residues contain a single arm-like domain and are highly conserved in the presenilins, suggesting that they form a functional armadillo protein binding site for the presenilins. ..
- Zhang L, Marcu M, Nau Staudt K, Trifaro J. Recombinant scinderin enhances exocytosis, an effect blocked by two scinderin-derived actin-binding peptides and PIP2. Neuron. 1996;17:287-96 pubmed..Results suggest that scinderin-evoked cortical F-actin disassembly is required for secretion and that scinderin is an important component of the exocytotic machinery. ..
- Dragulev B, Sira S, AbouHaidar M, Campbell J. Sequence analysis of putative E3 and fiber genomic regions of two strains of canine adenovirus type 1. Virology. 1991;183:298-305 pubmed..The suggestion is made that the attenuation of the CLL strain may be related to the nonfunctionality of its E3 gene product(s). ..
- Copland I, Post M. Stretch-activated signaling pathways responsible for early response gene expression in fetal lung epithelial cells. J Cell Physiol. 2007;210:133-43 pubmed..Thus, calcium mobilization may be a point of hierarchical integration of mechanotransduction in lung epithelial cells. ..
- Herrera Abreu M, Wang Q, Vachon E, Suzuki T, Chow C, Wang Y, et al. Tyrosine phosphatase SHP-2 regulates IL-1 signaling in fibroblasts through focal adhesions. J Cell Physiol. 2006;207:132-43 pubmed..We conclude that IL-1beta mediated maturation of focal adhesions is dependent on tyrosine phosphorylation of SHP-2 at Y542, leading to recruitment of Gab1, a process that may influence the downstream activation of ERK. ..
- Stambolic V, Suzuki A, de la Pompa J, Brothers G, Mirtsos C, Sasaki T, et al. Negative regulation of PKB/Akt-dependent cell survival by the tumor suppressor PTEN. Cell. 1998;95:29-39 pubmed..Our results show that PTEN may exert its role as a tumor suppressor by negatively regulating the PI3'K/PKB/Akt signaling pathway. ..
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