Experts and Doctors on escherichia coli in Liège, Wallonia, Belgium


Locale: Liège, Wallonia, Belgium
Topic: escherichia coli

Top Publications

  1. Rocaboy M, Herman R, Sauvage E, Remaut H, Moonens K, Terrak M, et al. The crystal structure of the cell division amidase AmiC reveals the fold of the AMIN domain, a new peptidoglycan binding domain. Mol Microbiol. 2013;90:267-77 pubmed publisher
    ..We also demonstrate the interaction between AmiC and NlpD by microscale thermophoresis and confirm the importance of the active site blocking alpha helix in the regulation of the amidase activity. ..
  2. Mainil J, Jacquemin E, Pohl P, Kaeckenbeeck A, Benz I. DNA sequences coding for the F18 fimbriae and AIDA adhesin are localised on the same plasmid in Escherichia coli isolates from piglets. Vet Microbiol. 2002;86:303-11 pubmed
    ..coli. Moreover the plasmid DNA hybridisation results suggest a localisation on the same plasmid of the aidA- and fed-related DNA sequences. ..
  3. Lassaux P, Traore D, Loisel E, Favier A, Docquier J, Sohier J, et al. Biochemical and structural characterization of the subclass B1 metallo-?-lactamase VIM-4. Antimicrob Agents Chemother. 2011;55:1248-55 pubmed publisher
    ..Study of the thermal stability and the activity of the holo- and apo-VIM-4 enzymes revealed that Zn²+ ions have a pronounced stabilizing effect on the enzyme and are necessary for preserving the structure. ..
  4. Kerff F, Petrella S, Mercier F, Sauvage E, Herman R, Pennartz A, et al. Specific structural features of the N-acetylmuramoyl-L-alanine amidase AmiD from Escherichia coli and mechanistic implications for enzymes of this family. J Mol Biol. 2010;397:249-59 pubmed publisher
    ..Analysis of the different structures reveals the similarity between the catalytic mechanism of zinc amidases of the AmiD family and the thermolysin-related zinc peptidases. ..
  5. Zervosen A, Saegerman C, Antoniotti I, Robert B, Ruth N, Collard A, et al. Characterization of the cattle serum antibody responses against TEM beta-lactamase and the nonimmunogenic Escherichia coli heat-stable enterotoxin (STaI). FEMS Immunol Med Microbiol. 2008;54:319-29 pubmed publisher
    ..In contrast, a strong antibody response against TEM-1 beta-lactamase was demonstrated. ..
  6. Steukers M, Schaus J, van Gool R, Hoyoux A, Richalet P, Sexton D, et al. Rapid kinetic-based screening of human Fab fragments. J Immunol Methods. 2006;310:126-35 pubmed
    ..The procedure allows up to 100 clones per week to be screened and permits the identification of a small number of high-affinity Fabs from a large batch obtained following library selection or affinity maturation. ..
  7. Zervosen A, Lu W, Chen Z, White R, Demuth T, Frere J. Interactions between penicillin-binding proteins (PBPs) and two novel classes of PBP inhibitors, arylalkylidene rhodanines and arylalkylidene iminothiazolidin-4-ones. Antimicrob Agents Chemother. 2004;48:961-9 pubmed
    ..The inhibition of PBP 2xS by several of them appeared to be noncompetitive. The dissociation constant for the best inhibitor (Ki = 10 microM) was not influenced by the presence of the substrate. ..
  8. Delhalle L, Saegerman C, Farnir F, Korsak N, Maes D, Messens W, et al. Salmonella surveillance and control at post-harvest in the Belgian pork meat chain. Food Microbiol. 2009;26:265-71 pubmed publisher
    ..21+/-0.50 to 1.23+/-0.89 log CFU/g and from 1.33+/-0.58 to 2.78+/-0.43 log CFU/g, respectively. The results showed that faecal contamination still needs to be reduced, especially in specific individual plants. ..
  9. Raskin C, Gerard C, Donfut S, Giannotta E, Van Driessche G, Van Beeumen J, et al. BlaB, a protein involved in the regulation of Streptomyces cacaoi beta-lactamases, is a penicillin-binding protein. Cell Mol Life Sci. 2003;60:1460-9 pubmed
    ..BlaB could be acylated by some of them, and it can therefore be considered as a penicillin-binding protein. BlaB is devoid of beta-lactamase, D-aminopeptidase, DD-carboxypeptidase or thiolesterase activity. ..

More Information


  1. Szalo I, Goffaux F, Pirson V, Pierard D, Ball H, Mainil J. Presence in bovine enteropathogenic (EPEC) and enterohaemorrhagic (EHEC) Escherichia coli of genes encoding for putative adhesins of human EHEC strains. Res Microbiol. 2002;153:653-8 pubmed
    ..Overall results showed that different genes encoding for putative adhesins of human EHEC strains are present in bovine EPEC and EHEC strains, but not one of them is present in all strains. ..
  2. Bebrone C, Anne C, De Vriendt K, Devreese B, Rossolini G, Van Beeumen J, et al. Dramatic broadening of the substrate profile of the Aeromonas hydrophila CphA metallo-beta-lactamase by site-directed mutagenesis. J Biol Chem. 2005;280:28195-202 pubmed
    ..The double mutant is significantly activated by the binding of a second zinc ion under conditions where the WT enzyme is non-competitively inhibited by the same ion. ..
  3. Makarchikov A, Brans A, Bettendorff L. Thiamine diphosphate adenylyl transferase from E. coli: functional characterization of the enzyme synthesizing adenosine thiamine triphosphate. BMC Biochem. 2007;8:17 pubmed
    ..The existence of a mechanism for the enzymatic synthesis of this compound is in agreement with the hypothesis of a non-cofactor role for thiamine derivatives in living cells. ..
  4. Gigliobianco T, Lakaye B, Makarchikov A, Wins P, Bettendorff L. Adenylate kinase-independent thiamine triphosphate accumulation under severe energy stress in Escherichia coli. BMC Microbiol. 2008;8:16 pubmed publisher
    ..Furthermore, they show that E. coli accumulate large amounts of ThTP under severe energy stress when ATP levels are very low, an observation not in favor of an ATP-dependent mechanisms for ThTP synthesis. ..
  5. Sauvage E, Powell A, Heilemann J, Josephine H, Charlier P, Davies C, et al. Crystal structures of complexes of bacterial DD-peptidases with peptidoglycan-mimetic ligands: the substrate specificity puzzle. J Mol Biol. 2008;381:383-93 pubmed publisher
    ..This striking deficiency may represent a sophisticated defense mechanism against low-molecular-mass substrate-analogue inhibitors/antibiotics; its discovery should focus new inhibitor design...
  6. Pennartz A, Généreux C, Parquet C, Mengin Lecreulx D, Joris B. Substrate-induced inactivation of the Escherichia coli AmiD N-acetylmuramoyl-L-alanine amidase highlights a new strategy to inhibit this class of enzyme. Antimicrob Agents Chemother. 2009;53:2991-7 pubmed publisher
    ..The soluble truncated AmiD exhibits a biphasic kinetic time course that can be explained by the inactivation of the enzyme by the substrate. This behavior highlights a new strategy to inhibit this class of enzymes. ..
  7. Gigliobianco T, Lakaye B, Wins P, El Moualij B, Zorzi W, Bettendorff L. Adenosine thiamine triphosphate accumulates in Escherichia coli cells in response to specific conditions of metabolic stress. BMC Microbiol. 2010;10:148 pubmed publisher
    ..Both conditions prevent bacterial growth. There is no obvious link with the stringent response or catabolite repression. ..
  8. Kohn G, Delvaux D, Lakaye B, Servais A, Scholer G, Fillet M, et al. High inorganic triphosphatase activities in bacteria and mammalian cells: identification of the enzymes involved. PLoS ONE. 2012;7:e43879 pubmed publisher
    ..The role of these enzymes may be to hydrolyze PPP(i), which could be cytotoxic because of its high affinity for Ca(2+), thereby interfering with Ca(2+) signaling. ..
  9. Magoué C, Melin P, Gangoue Pieboji J, Okomo Assoumou M, Boreux R, de Mol P. Prevalence and spread of extended-spectrum ?-lactamase-producing Enterobacteriaceae in Ngaoundere, Cameroon. Clin Microbiol Infect. 2013;19:E416-20 pubmed publisher
    ..Our results highlight the importance of outpatients, inpatients, their carers, hospital workers and their families as reservoirs of ESBL-producing Enterobacteriaceae. ..
  10. Lakaye B, Makarchikov A, Antunes A, Zorzi W, Coumans B, De Pauw E, et al. Molecular characterization of a specific thiamine triphosphatase widely expressed in mammalian tissues. J Biol Chem. 2002;277:13771-7 pubmed
    ..The mRNA was expressed in most human tissues but at relatively low levels. This is the first report of a molecular characterization of a specific ThTPase. ..
  11. Rodríguez M, Herman R, Ghiglione B, Kerff F, D Amico González G, Bouillenne F, et al. Crystal structure and kinetic analysis of the class B3 di-zinc metallo-?-lactamase LRA-12 from an Alaskan soil metagenome. PLoS ONE. 2017;12:e0182043 pubmed publisher