Genomes and Genes
Experts and Doctors on animals in Namur, Wallonia, Belgium
Locale: Namur, Wallonia, Belgium
- Mayon N, Bertrand A, Leroy D, Malbrouck C, Mandiki S, Silvestre F, et al. Multiscale approach of fish responses to different types of environmental contaminations: a case study. Sci Total Environ. 2006;367:715-31 pubmed..By using a multiparametric approach, a better understanding of response mechanisms to pollution in fish may be achieved in order to improve the ecological status of river ecosystems. ..
- Vandenplas Y, De Greef E, Xinias I, Vrani O, Mavroudi A, Hammoud M, et al. Safety of a thickened extensive casein hydrolysate formula. Nutrition. 2016;32:206-12 pubmed publisher..Height and weight evolution was satisfactory throughout the 6 mo study. The eCHF fulfills the criteria of a hypoallergenic formula and the NT- and T-eCHF reduced CMA symptoms. Growth was within normal range. ..
- Delrue R, Lestrate P, Tibor A, Letesson J, De Bolle X. Brucella pathogenesis, genes identified from random large-scale screens. FEMS Microbiol Lett. 2004;231:1-12 pubmed..This has identified several more potential virulence factors for Brucella that were not found in large screens. Here, we present an overall view of Brucella virulence by compiling virulence data from the study of 184 attenuated mutants. ..
- Coulier B, Tancredi M, Ramboux A. Spiral CT and multidetector-row CT diagnosis of perforation of the small intestine caused by ingested foreign bodies. Eur Radiol. 2004;14:1918-25 pubmed..Moreover, the high specificity of the CT diagnosis made it possible to avoid surgerical exploration in three patients. ..
- Nadzialek S, Kestemont P. 17alpha-ethinylestradiol induces an imbalance between apoptosis and cell proliferation to sex steroid disruption in a testis culture of gudgeon, Gobio gobio. Environ Toxicol Chem. 2010;29:881-6 pubmed publisher..The results of the present study could be the basis conditions for oocyte development within the testis of a common teleost fish under xenoestrogen exposure. ..
- Bouaziz N, Redon M, Quéré L, Remacle J, Michiels C. Mitochondrial respiratory chain as a new target for anti-ischemic molecules. Eur J Pharmacol. 2002;441:35-45 pubmed..This anti-ischemic activity is probably due to a protection of complexes I and III of the mitochondrial respiratory chain. ..
- Thiry A, Ledecq M, Cecchi A, Dogne J, Wouters J, Supuran C, et al. Indanesulfonamides as carbonic anhydrase inhibitors. Toward structure-based design of selective inhibitors of the tumor-associated isozyme CA IX. J Med Chem. 2006;49:2743-9 pubmed..We describe here the first human CA IX model built by homology with another CA isozyme already crystallized. Docking studies were performed to explore the binding mode of our indanesulfonamide derivatives. ..
- Magnette A, Huang T, Renzi F, Bogaerts P, Cornelis G, Glupczynski Y. Improvement of identification of Capnocytophaga canimorsus by matrix-assisted laser desorption ionization-time of flight mass spectrometry using enriched database. Diagn Microbiol Infect Dis. 2016;84:12-15 pubmed publisher..MALDI-TOF MS can accurately identify C. canimorsus and C. cynodegmi using an enriched database and thus constitutes a valuable diagnostic tool in the clinical laboratory. ..
- Vankoningsloo S, Piens M, Lecocq C, Gilson A, De Pauw A, Renard P, et al. Mitochondrial dysfunction induces triglyceride accumulation in 3T3-L1 cells: role of fatty acid beta-oxidation and glucose. J Lipid Res. 2005;46:1133-49 pubmed..Taken together, these results could explain how mitochondrial impairment leads to the multivesicular phenotype found in some mitochondria-originating diseases associated with a dysfunction in fat metabolism. ..
- Letesson J, Tibor A, Van Eynde G, Wansard V, Weynants V, Denoel P, et al. Humoral immune responses of Brucella-infected cattle, sheep, and goats to eight purified recombinant Brucella proteins in an indirect enzyme-linked immunosorbent assay. Clin Diagn Lab Immunol. 1997;4:556-64 pubmed..None of the recombinant antigens detected experimentally infected nonpregnant cows and sheep or naturally infected cattle. This study is a first step towards the development of a multiprotein diagnostic reagent for brucellosis. ..
- Duterme C, Mertens Strijthagen J, Tammi M, Flamion B. Two novel functions of hyaluronidase-2 (Hyal2) are formation of the glycocalyx and control of CD44-ERM interactions. J Biol Chem. 2009;284:33495-508 pubmed publisher..These effects may be relevant to the purported in vivo tumor-suppressive activity of Hyal2...
- Tejerina S, De Pauw A, Vankoningsloo S, Houbion A, Renard P, de Longueville F, et al. Mild mitochondrial uncoupling induces 3T3-L1 adipocyte de-differentiation by a PPARgamma-independent mechanism, whereas TNFalpha-induced de-differentiation is PPARgamma dependent. J Cell Sci. 2009;122:145-55 pubmed publisher..These results highlight some new mechanisms that might potentially be involved in adipocyte de-differentiation initiated by a mitochondrial uncoupling...
- Beckers M, Bar I, Huynh Thu T, Dernoncourt C, Brunialti A, Montagutelli X, et al. A high-resolution genetic map of mouse chromosome 5 encompassing the reeler (rl) locus. Genomics. 1994;23:685-90 pubmed..5 and 1.0 cM, respectively (95% confidence level), suggesting that the region could be physically mapped using a manageable number of YAC clones. ..
- Dewez M, Bauer F, Dieu M, Raes M, Vandenhaute J, Hermand D. The conserved Wobble uridine tRNA thiolase Ctu1-Ctu2 is required to maintain genome integrity. Proc Natl Acad Sci U S A. 2008;105:5459-64 pubmed publisher..Thus, a translation defect due to unmodified tRNAs results in severe genome instability. ..
- Boonen M, Puissant E, Gilis F, Flamion B, Jadot M. Mouse liver lysosomes contain enzymatically active processed forms of Hyal-1. Biochem Biophys Res Commun. 2014;446:1155-60 pubmed publisher..The knockdown of Hyal-1 results in an 80% decrease of total acid hyaluronidase activity in the mouse liver, confirming that Hyal-1 is a key actor of HA catabolism in this organ. ..
- Vitry M, De Trez C, Goriely S, Dumoutier L, Akira S, Ryffel B, et al. Crucial role of gamma interferon-producing CD4+ Th1 cells but dispensable function of CD8+ T cell, B cell, Th2, and Th17 responses in the control of Brucella melitensis infection in mice. Infect Immun. 2012;80:4271-80 pubmed publisher..melitensis infection. On the whole, these results could improve our ability to develop protective vaccines or therapeutic treatments against brucellosis...
- Dernoncourt C, Ruelle D, Goffinet A. Estimation of genetic distances between "reeler" and nearby loci on mouse chromosome 5. Genomics. 1991;11:1167-9 pubmed..Together with previous work, these observations suggest the following gene order: Cen-Sor/Pgy-1-rl-T31H-En-2-other loci. ..
- Sobry S, Havelange A, Van Cutsem P. Immunocytochemistry of pectins in shoot apical meristems: consequences for intercellular adhesion. Protoplasma. 2005;225:15-22 pubmed..The type and distribution of pectins are discussed in relation to cellular adhesion between active meristem cells. ..
- Michel G, Minet E, Ernest I, Roland I, Durant F, Remacle J, et al. A model for the complex between the hypoxia-inducible factor-1 (HIF-1) and its consensus DNA sequence. J Biomol Struct Dyn. 2000;18:169-79 pubmed..From these results, we postulate that the specificity of HIF-1 toward its DNA sequence could be driven by the HIF-1alpha subunit. The predicted model will be verified by X-Ray currently ongoing. ..
- Bar I, Lambert de Rouvroit C, Goffinet A. The evolution of cortical development. An hypothesis based on the role of the Reelin signaling pathway. Trends Neurosci. 2000;23:633-8 pubmed..Thus, the Reelin pathway played a key role in cortical architectonic evolution in mammalian and squamate lineages. ..
- Bertrand M, Huijbers I, Chomez P, De Backer O. Comparative expression analysis of the MAGED genes during embryogenesis and brain development. Dev Dyn. 2004;230:325-34 pubmed..We observed a discrepancy between Maged1 expression levels of RNA and protein, suggesting that its expression is regulated at a posttranscriptional level during the mouse development. ..
- Xu X, Kestemont P. Lipid metabolism and FA composition in tissues of Eurasian perch Perca fluviatilis as influenced by dietary fats. Lipids. 2002;37:297-304 pubmed..The present study indicates that high tissue content of DHA in the muscle of Eurasian perch was attributable to the greater ability for n-3 acid bioconversion. ..
- Bar I, Lambert de Rouvroit C, Royaux I, Krizman D, Dernoncourt C, Ruelle D, et al. A YAC contig containing the reeler locus with preliminary characterization of candidate gene fragments. Genomics. 1995;26:543-9 pubmed
- Debortoli N, Li X, Eyres I, Fontaneto D, Hespeels B, Tang C, et al. Genetic Exchange among Bdelloid Rotifers Is More Likely Due to Horizontal Gene Transfer Than to Meiotic Sex. Curr Biol. 2016;26:723-32 pubmed publisher..Our findings suggest that bdelloids evolve asexually but exchange DNA horizontally both within and between species. ..
- Santilman V, Jadot M, Mainferme F. Importance of the propeptide in the biosynthetic maturation of rat cathepsin C. Eur J Cell Biol. 2002;81:654-63 pubmed..These results demonstrate that in addition to its role as a chaperone or in maintaining the latency of the enzymatic activity, the propeptide is required for proper transport and expression of newly synthesized cathepsin C. ..
- Nadzialek S, Depiereux S, Mandiki S, Kestemont P. In vivo biomarkers of estrogenicity: limitation of interpretation in wild environment. Arch Environ Contam Toxicol. 2011;60:471-8 pubmed publisher..Even if powerful biomarkers of estrogenicty were developed, the interpretation of the response profile remains limited through in vivo parameters due to the complex association between compounds of various origins...
- Douxfils J, Deprez M, Mandiki S, Milla S, Henrotte E, Mathieu C, et al. Physiological and proteomic responses to single and repeated hypoxia in juvenile Eurasian perch under domestication--clues to physiological acclimation and humoral immune modulations. Fish Shellfish Immunol. 2012;33:1112-22 pubmed publisher
- Claine F, Coupeau D, Wiggers L, Muylkens B, Kirschvink N. Schmallenberg virus among female lambs, Belgium, 2012. Emerg Infect Dis. 2013;19:1115-7 pubmed publisher..Bimonthly assessment by quantitative reverse transcription PCR and seroneutralization demonstrated that 100% of lambs were infected. Viremia duration may be longer in naturally infected than in experimentally infected animals. ..
- Bertrand L, Vertommen D, Depiereux E, Hue L, Rider M, Feytmans E. Modelling the 2-kinase domain of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase on adenylate kinase. Biochem J. 1997;321 ( Pt 3):615-21 pubmed..It accommodates all the results of site-directed mutagenesis studies carried out to date on residues in the 2-kinase domain. It also allows residues potentially involved in catalysis and/or substrate binding to be predicted. ..
- Royaux I, Bernier B, Montgomery J, Flaherty L, Goffinet A. Reln(rl-Alb2), an allele of reeler isolated from a chlorambucil screen, is due to an IAP insertion with exon skipping. Genomics. 1997;42:479-82 pubmed..At the genomic level, the mutation was shown to be due to an intracisternal A-particle insertion leading to exon skipping. This appears to be the first observation of retrotransposon insertion during chlorambucil mutagenesis. ..
- Vitry M, Hanot Mambres D, De Trez C, Akira S, Ryffel B, Letesson J, et al. Humoral immunity and CD4+ Th1 cells are both necessary for a fully protective immune response upon secondary infection with Brucella melitensis. J Immunol. 2014;192:3740-52 pubmed publisher..In summary, our work identifies several immune markers closely associated with protective immune memory and could help to define a rational strategy to obtain an effective human vaccine against brucellosis...
- Copin R, De Baetselier P, Carlier Y, Letesson J, Muraille E. MyD88-dependent activation of B220-CD11b+LY-6C+ dendritic cells during Brucella melitensis infection. J Immunol. 2007;178:5182-91 pubmed..Taken together, our results provide new insight into how immune responses fight Brucella infection. ..
- Lambert de Rouvroit C, Bernier B, Royaux I, de Bergeyck V, Goffinet A. Evolutionarily conserved, alternative splicing of reelin during brain development. Exp Neurol. 1999;156:229-38 pubmed..Both alternative splicing events are present in mouse, rat, and man, suggesting that the corresponding reelin forms are functionally important. ..
- Delrue R, Deschamps C, Léonard S, Nijskens C, Danese I, Schaus J, et al. A quorum-sensing regulator controls expression of both the type IV secretion system and the flagellar apparatus of Brucella melitensis. Cell Microbiol. 2005;7:1151-61 pubmed..In agreement with this hypothesis, we observed that, as virB operon, flagellar genes are controlled by the pheromone. All together these data support a model in which VjbR acts as a major regulator of virulence factors in Brucella...
- Kuvbachieva A, Bestel A, Tissir F, Maloum I, Guimiot F, Ramoz N, et al. Identification of a novel brain-specific and Reelin-regulated gene that encodes a protein colocalized with synapsin. Eur J Neurosci. 2004;20:603-10 pubmed..When expressed as a GFP fusion protein in transfected non-neuronal cells and primary neurons, this protein localizes, respectively, to the nuclear membrane or axonal outgrowths, indicating a function in axonal traffic or signalling. ..
- Bauer F, Hermand D. A coordinated codon-dependent regulation of translation by Elongator. Cell Cycle. 2012;11:4524-9 pubmed publisher..Our recent proteome-wide study in fission yeast supports a coordinated codon-dependent regulation of translation by Elongator. Here we provide additional analyses extending this hypothesis to budding yeast and worm. ..
- Flot J, Hespeels B, Li X, Noel B, Arkhipova I, Danchin E, et al. Genomic evidence for ameiotic evolution in the bdelloid rotifer Adineta vaga. Nature. 2013;500:453-7 pubmed publisher..Furthermore, 8% of the genes are likely to be of non-metazoan origin and were probably acquired horizontally. This apparent convergence between bdelloids and prokaryotes sheds new light on the evolutionary significance of sex. ..
- Mirabella A, Terwagne M, Zygmunt M, Cloeckaert A, De Bolle X, Letesson J. Brucella melitensis MucR, an orthologue of Sinorhizobium meliloti MucR, is involved in resistance to oxidative, detergent, and saline stresses and cell envelope modifications. J Bacteriol. 2013;195:453-65 pubmed publisher..These data reinforce the idea that there is a common conserved circuitry between plant symbionts and animal pathogens that regulates the relationship they have with their hosts...
- Jadin L, Wu X, Ding H, Frost G, Onclinx C, Triggs Raine B, et al. Skeletal and hematological anomalies in HYAL2-deficient mice: a second type of mucopolysaccharidosis IX?. FASEB J. 2008;22:4316-26 pubmed publisher..In addition, the viability of HYAL2-deficient mice raises the possibility that a similar defect, defining a new MPS disorder, exists in humans. ..
- Dandoy P, Meunier C, Michiels C, Su B. Hybrid shell engineering of animal cells for immune protections and regulation of drug delivery: towards the design of "artificial organs". PLoS ONE. 2011;6:e20983 pubmed publisher..e. insulin), substituting the declining organ functions of the patient. ..
- Vankoningsloo S, Piret J, Saout C, Noel F, Mejia J, Zouboulis C, et al. Cytotoxicity of multi-walled carbon nanotubes in three skin cellular models: effects of sonication, dispersive agents and corneous layer of reconstructed epidermis. Nanotoxicology. 2010;4:84-97 pubmed publisher..However, the cytotoxic effects of raw and sonicated nanotubes could be prevented in presence of dispersive agents. No cytotoxic effects were observed in SZ95 sebocytes or in stratified epidermises reconstructed in vitro...
- Gasingirwa M, Thirion J, Mertens Strijthagen J, Wattiaux de Coninck S, Flamion B, Wattiaux R, et al. Endocytosis of hyaluronidase-1 by the liver. Biochem J. 2010;430:305-13 pubmed publisher..Although its degradation half-time is short, Hyal-1 could exert its activity due to a constant supply of active molecules from the blood. ..
- Calay D, Rousseau A, Mattart L, Nuyens V, Delporte C, Van Antwerpen P, et al. Copper and myeloperoxidase-modified LDLs activate Nrf2 through different pathways of ROS production in macrophages. Antioxid Redox Signal. 2010;13:1491-502 pubmed publisher..These data clearly suggest that results obtained until now with copper oxidized-LDLs should be carefully reevaluated, taking into consideration physiologically more relevant oxidized LDLs. ..
- Mendes S, Candi A, Vansteenbrugge M, Pignon M, Bult H, Boudjeltia K, et al. Microarray analyses of the effects of NF-kappaB or PI3K pathway inhibitors on the LPS-induced gene expression profile in RAW264.7 cells: synergistic effects of rapamycin on LPS-induced MMP9-overexpression. Cell Signal. 2009;21:1109-22 pubmed publisher..Our results suggest a new pathway involving mTOR for regulating specifically mmp9 in LPS-stimulated RAW264.7 cells. ..
- Stegen G, Pasmans F, Schmidt B, Rouffaer L, Van Praet S, Schaub M, et al. Drivers of salamander extirpation mediated by Batrachochytrium salamandrivorans. Nature. 2017;544:353-356 pubmed publisher..The combined characteristics of the disease ecology suggest that further expansion of this fungus will behave as a 'perfect storm' that is able to rapidly extirpate highly susceptible salamander populations across Europe. ..
- Blanchard G, Gardeur J, Mathis N, Brun Bellut J, Kestemont P. Ultrastructural features of hepatocytes in cultured Eurasian perch ( Perca fluviatilis L.) as affected by nutritional and husbandry conditions. Br J Nutr. 2008;100:317-31 pubmed publisher..However, factors that could be pooled under the designation of factors leading to food accessibility and lipid and protein quality intensify or compensate the effect of temperature. ..
- Wery M, Shematorova E, Van Driessche B, Vandenhaute J, Thuriaux P, Van Mullem V. Members of the SAGA and Mediator complexes are partners of the transcription elongation factor TFIIS. EMBO J. 2004;23:4232-42 pubmed..It is proposed that TFIIS and the Spt8-containing form of SAGA co-operate to rescue RNA polymerase II from unproductive elongation complexes, and that the Cdk8 module temporarily blocks transcription during transcript cleavage. ..
- Denoel P, Zygmunt M, Weynants V, Tibor A, Lichtfouse B, Briffeuil P, et al. Cloning and sequencing of the bacterioferritin gene of Brucella melitensis 16M strain. FEBS Lett. 1995;361:238-42 pubmed..The sequence of the Brucella bacterioferritin is 69% similar to that of E. coli, and many of the ferroxidase centre and haem-ligation residues are conserved. ..
- Goffinet A. The reeler gene: a clue to brain development and evolution. Int J Dev Biol. 1992;36:101-7 pubmed..The characterization and thus the cloning of the reeler gene is therefore important for our understanding of brain development. Recent data on the fine chromosomal mapping of the mutation prior to its positional cloning are reported. ..
- Demine S, Tejerina S, Bihin B, Thiry M, Reddy N, Renard P, et al. Mild mitochondrial uncoupling induces HSL/ATGL-independent lipolysis relying on a form of autophagy in 3T3-L1 adipocytes. J Cell Physiol. 2018;233:1247-1265 pubmed publisher..This new mechanism of triacylglycerol breakdown in adipocytes exposed to mild uncoupling provides new insights on the biology of adipocytes dealing with mitochondria forced to dissipate energy. ..
- Boonen M, Staudt C, Gilis F, Oorschot V, Klumperman J, Jadot M. Cathepsin D and its newly identified transport receptor SEZ6L2 can modulate neurite outgrowth. J Cell Sci. 2016;129:557-68 pubmed publisher..Taken together, our findings highlight that SEZ6L2 can serve as receptor to mediate the sorting of cathepsin D to endosomes, and suggest that proteolytic cleavage of SEZ6L2 by cathepsin D modulates neuronal differentiation. ..
- Declèves A, Caron N, Voisin V, Legrand A, Bouby N, Kultti A, et al. Synthesis and fragmentation of hyaluronan in renal ischaemia. Nephrol Dial Transplant. 2012;27:3771-81 pubmed publisher..The current data open the door to timed pharmacological interventions blocking the production of HA fragments. ..
- Sermeus A, Michiels C. Reciprocal influence of the p53 and the hypoxic pathways. Cell Death Dis. 2011;2:e164 pubmed publisher..Finally, the synergistic or antagonistic effects of p53 and HIF-1 on some important cellular pathways are discussed. ..
- Cosse J, Michiels C. Tumour hypoxia affects the responsiveness of cancer cells to chemotherapy and promotes cancer progression. Anticancer Agents Med Chem. 2008;8:790-7 pubmed..The aim of this review is to provide a description of known hypoxia-induced mechanisms of chemoresistance at a cellular level. ..
- Bertrand M, Kenchappa R, Andrieu D, Leclercq Smekens M, Nguyen H, Carter B, et al. NRAGE, a p75NTR adaptor protein, is required for developmental apoptosis in vivo. Cell Death Differ. 2008;15:1921-9 pubmed publisher..Together, these studies demonstrate that NRAGE plays an important role in apoptotic-signaling in vivo. ..
- Arnould T, Mercy L, Houbion A, Vankoningsloo S, Renard P, Pascal T, et al. mtCLIC is up-regulated and maintains a mitochondrial membrane potential in mtDNA-depleted L929 cells. FASEB J. 2003;17:2145-7 pubmed..These findings suggest that mtCLIC could be involved in mitochondrial membrane potential generation in mtDNA-depleted cells, a feature required to prevent apoptosis and to drive continuous protein import into mitochondria. ..
- Goffinet A, Dernoncourt C. Localization of the reeler gene relative to flanking loci on mouse chromosome 5. Mamm Genome. 1991;1:100-3 pubmed..Together with recently published evidence (Martin et al. 1989), our data suggest the following order: Cen-rl/Pgy-1-T31H-En-2. ..