Experts and Doctors on molecular cloning in Leuven, Flanders, Belgium


Locale: Leuven, Flanders, Belgium
Topic: molecular cloning

Top Publications

  1. Reumer A, Bogaerts A, Van Loy T, Husson S, Temmerman L, Choi C, et al. Unraveling the protective effect of a Drosophila phosphatidylethanolamine-binding protein upon bacterial infection by means of proteomics. Dev Comp Immunol. 2009;33:1186-95 pubmed publisher
    ..This is the first report demonstrating an immune function for a Drosophila PEBP protein. ..
  2. Dode L, De Greef C, Mountian I, Attard M, Town M, Casteels R, et al. Structure of the human sarco/endoplasmic reticulum Ca2+-ATPase 3 gene. Promoter analysis and alternative splicing of the SERCA3 pre-mRNA. J Biol Chem. 1998;273:13982-94 pubmed
    ..Expression of the mouse SERCA3 isoforms in COS-1 cells demonstrated their ability to function as active pumps, although with different apparent affinities for Ca2+. ..
  3. Veugelers M, Vermeesch J, Watanabe K, Yamaguchi Y, Marynen P, David G. GPC4, the gene for human K-glypican, flanks GPC3 on xq26: deletion of the GPC3-GPC4 gene cluster in one family with Simpson-Golabi-Behmel syndrome. Genomics. 1998;53:1-11 pubmed
    ..The tight clustering of GPC3 and GPC4, with deletions that occasionally affect both genes, may be relevant for explaining the variability of the SGBS phenotype. ..
  4. Ma P, Winderickx J, Nauwelaers D, Dumortier F, De Doncker A, Thevelein J, et al. Deletion of SFI1, a novel suppressor of partial Ras-cAMP pathway deficiency in the yeast Saccharomyces cerevisiae, causes G(2) arrest. Yeast. 1999;15:1097-109 pubmed
    ..In conclusion, we have identified an essential function in the G(2)-M transition of the cell cycle for a yeast gene of previously unknown function. ..
  5. Beckers M, Gabriels J, van der Maarel S, De Vriese A, Frants R, Collen D, et al. Active genes in junk DNA? Characterization of DUX genes embedded within 3.3 kb repeated elements. Gene. 2001;264:51-7 pubmed
    ..Our results indicate that active genes of unusual structure are present in chromosome regions characterized by large amounts of heterochromatic repetitive DNA. ..
  6. Castermans D, Vermeesch J, Fryns J, Steyaert J, Van de Ven W, Creemers J, et al. Identification and characterization of the TRIP8 and REEP3 genes on chromosome 10q21.3 as novel candidate genes for autism. Eur J Hum Genet. 2007;15:422-31 pubmed
    ..In addition, our data indicate that in the selection of positional candidate genes when studying chromosomal aberrations, position effects should be taken into account. ..
  7. Kas K, Lemahieu V, Meyen E, Van de Ven W, Merregaert J. Isolation, cDNA, and genomic structure of a conserved gene (NOF) at chromosome 11q13 next to FAU and oriented in the opposite transcriptional orientation. Genomics. 1996;34:433-6 pubmed
    ..Moreover, the mouse ESTs map in the same organization, closely linked to the FAU gene, in the mouse genome. NOF, however, is not affected by the t(11;17)(q13;q21) chromosomal translocation. ..
  8. Van den Bergh K, Rouge P, Proost P, Coosemans J, Krouglova T, Engelborghs Y, et al. Synergistic antifungal activity of two chitin-binding proteins from spindle tree (Euonymus europaeus L.). Planta. 2004;219:221-32 pubmed
  9. Frints S, Jun L, Fryns J, Devriendt K, Teulingkx R, Van den Berghe L, et al. Inv(X)(p21.1;q22.1) in a man with mental retardation, short stature, general muscle wasting, and facial dysmorphism: clinical study and mutation analysis of the NXF5 gene. Am J Med Genet A. 2003;119A:367-74 pubmed
    ..Therefore, mutation screening of the NXF gene family in phenotypically identical patients is recommended. ..

More Information


  1. Lindemans M, Janssen T, Husson S, Meelkop E, Temmerman L, Clynen E, et al. A neuromedin-pyrokinin-like neuropeptide signaling system in Caenorhabditis elegans. Biochem Biophys Res Commun. 2009;379:760-4 pubmed publisher
    ..In vertebrates, NMU is the putative homolog of a protostomian CAPA-PK. ..
  2. Simonet G, Breugelmans B, Proost P, Claeys I, Van Damme J, De Loof A, et al. Characterization of two novel pacifastin-like peptide precursor isoforms in the desert locust (Schistocerca gregaria): cDNA cloning, functional analysis and real-time RT-PCR gene expression studies. Biochem J. 2005;388:281-9 pubmed
  3. Rector A, Tachezy R, Van Doorslaer K, MacNamara T, Burk R, Sundberg J, et al. Isolation and cloning of a papillomavirus from a North American porcupine by using multiply primed rolling-circle amplification: the Erethizon dorsatum papillomavirus type 1. Virology. 2005;331:449-56 pubmed publisher
    ..EdPV-1 is only distantly related to other benign cutaneous papillomavirus sequences and is the first member of the novel Sigma papillomavirus genus...
  4. Hens K, Macours N, Claeys I, Francis C, Huybrechts R. Cloning and expression of the yolk protein of the tsetse fly Glossina morsitans morsitans. Insect Biochem Mol Biol. 2004;34:1281-7 pubmed
    ..Northern blot analysis showed that only the ovarian follicle cells, and not the fat body represents the site of yolk protein synthesis. ..
  5. Lavigne R, Burkal tseva M, Robben J, Sykilinda N, Kurochkina L, Grymonprez B, et al. The genome of bacteriophage phiKMV, a T7-like virus infecting Pseudomonas aeruginosa. Virology. 2003;312:49-59 pubmed
    ..Also, the strong virulence toward many clinical P. aeruginosa isolates and a short replication time make phiKMV attractive for phage therapy or a potential source for antimicrobial proteins...
  6. Frints S, Marynen P, Hartmann D, Fryns J, Steyaert J, Schachner M, et al. CALL interrupted in a patient with non-specific mental retardation: gene dosage-dependent alteration of murine brain development and behavior. Hum Mol Genet. 2003;12:1463-74 pubmed
    ..This suggests that the CALL gene at 3p26.3 is a prime candidate for an autosomal form of mental retardation. So far, mutation analysis of the CALL gene in patients with non-specific MR did not reveal any disease-associated mutations. ..
  7. Freson K, Thys C, Wittewrongel C, Vermylen J, Hoylaerts M, Van Geet C. Molecular cloning and characterization of the GATA1 cofactor human FOG1 and assessment of its binding to GATA1 proteins carrying D218 substitutions. Hum Genet. 2003;112:42-9 pubmed
    ..The identification of the human FOG1 gene will enable the genetic screening of patients with non X-linked thrombocytopenia and dyserythropoiesis. ..
  8. Van Veldhoven P, Gijsbers S, Mannaerts G, Vermeesch J, Brys V. Human sphingosine-1-phosphate lyase: cDNA cloning, functional expression studies and mapping to chromosome 10q22(1). Biochim Biophys Acta. 2000;1487:128-34 pubmed
    ..Northern analysis showed the presence of rare large-sized mRNAs of 6.7, 5.8 and 4 kb and the highest expression in liver. By fluorescent in situ hybridization, the gene was mapped to chromosome 10q22. ..
  9. Baens M, Marynen P. A human homologue (BICD1) of the Drosophila bicaudal-D gene. Genomics. 1997;45:601-6 pubmed
    ..The conserved structural characteristics of the BICD1 protein and its expression in muscle and especially brain suggest that BICD1 is a component of a cytoskeleton-based mRNA sorting mechanism conserved during evolution. ..
  10. Mertens I, Meeusen T, Huybrechts R, De Loof A, Schoofs L. Characterization of the short neuropeptide F receptor from Drosophila melanogaster. Biochem Biophys Res Commun. 2002;297:1140-8 pubmed
  11. Rector A, Mostmans S, Van Doorslaer K, McKnight C, Maes R, Wise A, et al. Genetic characterization of the first chiropteran papillomavirus, isolated from a basosquamous carcinoma in an Egyptian fruit bat: the Rousettus aegyptiacus papillomavirus type 1. Vet Microbiol. 2006;117:267-75 pubmed publisher
    ..This is the first time a papillomavirus has been isolated and characterized from a member of the Chiroptera order...
  12. De Nys K, Meyhi E, Mannaerts G, Fransen M, Van Veldhoven P. Characterisation of human peroxisomal 2,4-dienoyl-CoA reductase. Biochim Biophys Acta. 2001;1533:66-72 pubmed
    ..Hence, the reductase does not seem to constitute a rate limiting step in the peroxisomal degradation of docosahexaenoic acid. The reduction of docosaheptaenoyl-CoA, however, was severely decreased in the presence of albumin. ..