Experts and Doctors on saccharomyces cerevisiae in Graz, Styria, Austria


Locale: Graz, Styria, Austria
Topic: saccharomyces cerevisiae

Top Publications

  1. Buttner S, Eisenberg T, Herker E, Carmona Gutierrez D, Kroemer G, Madeo F. Why yeast cells can undergo apoptosis: death in times of peace, love, and war. J Cell Biol. 2006;175:521-5 pubmed
    ..U. Frohlich, S. Wissing, S. Buttner, M. Fehr, S. Sigrist, and F. Madeo. 2004. J. Cell Biol. 164:501-507, underscoring the evolutionary benefit of a cell suicide program in yeast and, thus, giving a unicellular organism causes to die for. ..
  2. Wriessnegger T, Pichler H. Yeast metabolic engineering--targeting sterol metabolism and terpenoid formation. Prog Lipid Res. 2013;52:277-93 pubmed publisher
  3. Buttner S, Delay C, Franssens V, Bammens T, Ruli D, Zaunschirm S, et al. Synphilin-1 enhances ?-synuclein aggregation in yeast and contributes to cellular stress and cell death in a Sir2-dependent manner. PLoS ONE. 2010;5:e13700 pubmed publisher
    ..Synphilin-1 thereby induces cytotoxicity, an effect most pronounced for the wild-type protein and mediated via Sir2-dependent processes. ..
  4. Connerth M, Czabany T, Wagner A, Zellnig G, Leitner E, Steyrer E, et al. Oleate inhibits steryl ester synthesis and causes liposensitivity in yeast. J Biol Chem. 2010;285:26832-41 pubmed publisher
    ..In summary, our data showed that exposure to oleate led to disturbed lipid and membrane homeostasis along with liposensitivity of the yeast. ..
  5. Schnabl M, Daum G, Pichler H. Multiple lipid transport pathways to the plasma membrane in yeast. Biochim Biophys Acta. 2005;1687:130-40 pubmed
    ..However, efficient sec blocks did not abrogate protein and lipid transport, suggesting that parallel pathway(s) for the translocation of membrane components to the plasma membrane of yeast must exist. ..
  6. Carmona Gutierrez D, Bauer M, Ring J, Knauer H, Eisenberg T, Buttner S, et al. The propeptide of yeast cathepsin D inhibits programmed necrosis. Cell Death Dis. 2011;2:e161 pubmed publisher
    ..Altogether, these data discriminate two pro-survival functions of yeast CatD and provide first insight into the physiological regulation of programmed necrosis in yeast. ..
  7. Zweytick D, Hrastnik C, Kohlwein S, Daum G. Biochemical characterization and subcellular localization of the sterol C-24(28) reductase, erg4p, from the yeast saccharomyces cerevisiae. FEBS Lett. 2000;470:83-7 pubmed
    ..We conclude that ergosterol biosynthesis is completed in the endoplasmic reticulum, and the final product is supplied from there to its membranous destinations. ..
  8. Pertschy B, Saveanu C, Zisser G, Lebreton A, Tengg M, Jacquier A, et al. Cytoplasmic recycling of 60S preribosomal factors depends on the AAA protein Drg1. Mol Cell Biol. 2007;27:6581-92 pubmed
    ..This early cytoplasmic release reaction defines a novel step in eukaryotic ribosome maturation. ..
  9. Schalli M, Wolfsgruber A, Gonzalez Santana A, Tysoe C, Fischer R, Stütz A, et al. C-5a-substituted validamine type glycosidase inhibitors. Carbohydr Res. 2017;440-441:1-9 pubmed publisher
    ..Products turned out selective for ?-galactosidases as well as ?-glucosidases. ..

More Information


  1. Wendler F, Bergler H, Prutej K, Jungwirth H, Zisser G, Kuchler K, et al. Diazaborine resistance in the yeast Saccharomyces cerevisiae reveals a link between YAP1 and the pleiotropic drug resistance genes PDR1 and PDR3. J Biol Chem. 1997;272:27091-8 pubmed
    ..These results provide the first evidence for a functional link between the Yap1p-dependent stress response pathway and Pdr1p/Pdr3p-dependent development of pleiotropic drug resistance. ..
  2. Carmona Gutierrez D, Eisenberg T, Buttner S, Meisinger C, Kroemer G, Madeo F. Apoptosis in yeast: triggers, pathways, subroutines. Cell Death Differ. 2010;17:763-73 pubmed publisher
    ..Here, we provide an overview of the current knowledge about the apoptotic subroutine of yeast PCD and its regulation. ..
  3. Eisenberg T, Knauer H, Schauer A, Buttner S, Ruckenstuhl C, Carmona Gutierrez D, et al. Induction of autophagy by spermidine promotes longevity. Nat Cell Biol. 2009;11:1305-14 pubmed publisher
    ..Finally, we found that enhanced autophagy is crucial for polyamine-induced suppression of necrosis and enhanced longevity...
  4. Wriessnegger T, Sunga A, Cregg J, Daum G. Identification of phosphatidylserine decarboxylases 1 and 2 from Pichia pastoris. FEMS Yeast Res. 2009;9:911-22 pubmed publisher
    ..In summary, this study provides first insight into membrane manipulation of P. pastoris, which may serve as a useful method to modify cell biological properties of this microorganism for biotechnological purposes. ..
  5. Neuwirth M, Strohmeier M, Windeisen V, Wallner S, Deller S, Rippe K, et al. X-ray crystal structure of Saccharomyces cerevisiae Pdx1 provides insights into the oligomeric nature of PLP synthases. FEBS Lett. 2009;583:2179-86 pubmed publisher
    ..Further, we demonstrate that the yeast Pdx1 C-terminus contacts an adjacent subunit, and deletion of this segment decreases enzymatic activity 3.5-fold, suggesting a role in catalysis. ..
  6. Buttner S, Ruli D, Vögtle F, Galluzzi L, Moitzi B, Eisenberg T, et al. A yeast BH3-only protein mediates the mitochondrial pathway of apoptosis. EMBO J. 2011;30:2779-92 pubmed publisher
    ..Thus, the yeast genome encodes a functional BH3 domain that induces cell death through phylogenetically conserved mechanisms. ..
  7. Ruckenstuhl C, Buttner S, Carmona Gutierrez D, Eisenberg T, Kroemer G, Sigrist S, et al. The Warburg effect suppresses oxidative stress induced apoptosis in a yeast model for cancer. PLoS ONE. 2009;4:e4592 pubmed publisher
    ..Thus, the Warburg effect might directly contribute to the initiation of cancer formation--not only by enhanced glycolysis--but also via decreased respiration in the presence of oxygen, which suppresses apoptosis. ..
  8. Sollner S, Schober M, Wagner A, Prem A, Lorkova L, Palfey B, et al. Quinone reductase acts as a redox switch of the 20S yeast proteasome. EMBO Rep. 2009;10:65-70 pubmed publisher
    ..Thus, the Lot6-proteasome complex can be regarded as a redox switch in which the quinone reductase acts as a sensor for oxidative stress. ..
  9. Malanovic N, Streith I, Wolinski H, Rechberger G, Kohlwein S, Tehlivets O. S-adenosyl-L-homocysteine hydrolase, key enzyme of methylation metabolism, regulates phosphatidylcholine synthesis and triacylglycerol homeostasis in yeast: implications for homocysteine as a risk factor of atherosclerosis. J Biol Chem. 2008;283:23989-99 pubmed publisher
    ..The addition of homocysteine, by the reversal of the Sah1-catalyzed reaction, also leads to TG accumulation in yeast, providing an attractive model for the role of homocysteine as a risk factor of atherosclerosis in humans. ..
  10. Buttner S, Eisenberg T, Carmona Gutierrez D, Ruli D, Knauer H, Ruckenstuhl C, et al. Endonuclease G regulates budding yeast life and death. Mol Cell. 2007;25:233-46 pubmed
    ..Deletion of NUC1 diminishes apoptotic death when mitochondrial respiration is increased but enhances necrotic death when oxidative phosphorylation is repressed, pointing to dual--lethal and vital--roles for EndoG. ..
  11. Daum G, Tuller G, Nemec T, Hrastnik C, Balliano G, Cattel L, et al. Systematic analysis of yeast strains with possible defects in lipid metabolism. Yeast. 1999;15:601-14 pubmed
    ..Finally, lipid composition of mutant strains deleted of the respective open reading frames was determined. For some genes we found evidence suggesting a possible role in lipid metabolism. ..
  12. Spanova M, Zweytick D, Lohner K, Klug L, Leitner E, Hermetter A, et al. Influence of squalene on lipid particle/droplet and membrane organization in the yeast Saccharomyces cerevisiae. Biochim Biophys Acta. 2012;1821:647-53 pubmed publisher
  13. Koch K, Hromic A, Sorokina M, Strandback E, Reisinger M, Gruber K, et al. Structure, biochemical and kinetic properties of recombinant Pst2p from Saccharomyces cerevisiae, a FMN-dependent NAD(P)H:quinone oxidoreductase. Biochim Biophys Acta Proteins Proteom. 2017;1865:1046-1056 pubmed publisher
    ..In vivo experiments with a PST2 knock out and overexpressing strain demonstrated that Pst2p enables yeast cells to cope with quinone-induced damage suggesting a role of the enzyme in managing oxidative stress. ..
  14. Müllner H, Deutsch G, Leitner E, Ingolic E, Daum G. YEH2/YLR020c encodes a novel steryl ester hydrolase of the yeast Saccharomyces cerevisiae. J Biol Chem. 2005;280:13321-8 pubmed
  15. Carmona Gutierrez D, Alavian Ghavanini A, Habernig L, Bauer M, Hammer A, Rossmann C, et al. The cell death protease Kex1p is essential for hypochlorite-induced apoptosis in yeast. Cell Cycle. 2013;12:1704-12 pubmed publisher
  16. Glieder A, Weis R, Skranc W, Poechlauer P, Dreveny I, Majer S, et al. Comprehensive step-by-step engineering of an (R)-hydroxynitrile lyase for large-scale asymmetric synthesis. Angew Chem Int Ed Engl. 2003;42:4815-8 pubmed
  17. Jungwirth H, Wendler F, Platzer B, Bergler H, Högenauer G. Diazaborine resistance in yeast involves the efflux pumps Ycf1p and Flr1p and is enhanced by a gain-of-function allele of gene YAP1. Eur J Biochem. 2000;267:4809-16 pubmed
  18. Wolinski H, Kolb D, Hermann S, Koning R, Kohlwein S. A role for seipin in lipid droplet dynamics and inheritance in yeast. J Cell Sci. 2011;124:3894-904 pubmed publisher
  19. Horvath S, Wagner A, Steyrer E, Daum G. Metabolic link between phosphatidylethanolamine and triacylglycerol metabolism in the yeast Saccharomyces cerevisiae. Biochim Biophys Acta. 2011;1811:1030-7 pubmed publisher
    ..Moreover, it is very likely that local availability of PE in microsomes is crucial for TAG synthesis through the Lro1p reaction. ..
  20. Bauer M, Carmona Gutierrez D, Ruckenstuhl C, Reisenbichler A, Megalou E, Eisenberg T, et al. Spermidine promotes mating and fertilization efficiency in model organisms. Cell Cycle. 2013;12:346-52 pubmed publisher
  21. Hofbauer H, Schopf F, Schleifer H, Knittelfelder O, Pieber B, Rechberger G, et al. Regulation of gene expression through a transcriptional repressor that senses acyl-chain length in membrane phospholipids. Dev Cell. 2014;29:729-39 pubmed publisher
    ..These findings reveal an unexpected regulatory link between the major energy-sensing kinase, membrane lipid composition, and transcription. ..
  22. Galluzzi L, Baehrecke E, Ballabio A, Boya P, Bravo San Pedro J, Cecconi F, et al. Molecular definitions of autophagy and related processes. EMBO J. 2017;36:1811-1836 pubmed publisher
    ..The ultimate objective of this collaborative exchange is to formulate recommendations that facilitate the dissemination of knowledge within and outside the field of autophagy research. ..
  23. Madeo F, Tavernarakis N, Kroemer G. Can autophagy promote longevity?. Nat Cell Biol. 2010;12:842-6 pubmed publisher
    ..We summarize recent developments that outline these links and hypothesize that clearing cellular damage by autophagy is a common denominator of many lifespan-extending manipulations. ..
  24. Sollner S, Durchschlag M, Frohlich K, Macheroux P. The redox-sensing quinone reductase Lot6p acts as an inducer of yeast apoptosis. FEMS Yeast Res. 2009;9:885-91 pubmed publisher
    ..Thus, we propose that Lot6p is directly involved in the control of the apoptosis-like cell death in yeast. ..
  25. Wolinski H, Natter K, Kohlwein S. The fidgety yeast: focus on high-resolution live yeast cell microscopy. Methods Mol Biol. 2009;548:75-99 pubmed publisher
    ..If an image tells more than a thousand (perhaps misleading?) words, the ease of obtaining "images" thus rather suggests analyzing many thousands of images, to come up with one relevant and biologically significant conclusion...
  26. Madeo F, Durchschlag M, Kepp O, Panaretakis T, Zitvogel L, Frohlich K, et al. Phylogenetic conservation of the preapoptotic calreticulin exposure pathway from yeast to mammals. Cell Cycle. 2009;8:639-42 pubmed
    ..Altogether, our data point to the existence of an ancestral and cell death-related CRT exposure pathway with conserved elements shared between unicellular fungi and mammals. ..
  27. Wolinski H, Kohlwein S. Microscopic analysis of lipid droplet metabolism and dynamics in yeast. Methods Mol Biol. 2008;457:151-63 pubmed
    ..The applications and pitfalls of such techniques in understanding lipid storage and degradation are discussed...
  28. Paulitsch A, Weger W, Ginter Hanselmayer G, Marth E, Buzina W. A 5-year (2000-2004) epidemiological survey of Candida and non-Candida yeast species causing vulvovaginal candidiasis in Graz, Austria. Mycoses. 2006;49:471-5 pubmed
    ..glabrata and Saccharomyces cerevisiae. During a 1-year period 185 patients showed more than one episode of VVC. Patients aged 21-40 years were significantly more prone to suffer from VVC compared with other age-related groups...
  29. Tehlivets O, Hasslacher M, Kohlwein S. S-adenosyl-L-homocysteine hydrolase in yeast: key enzyme of methylation metabolism and coordinated regulation with phospholipid synthesis. FEBS Lett. 2004;577:501-6 pubmed
    ..Profound changes in cellular lipid composition upon depletion of Sah1p support the notion of a tight interaction between lipid metabolism and Sah1p function. ..
  30. Leber R, Fuchsbichler S, Klobucnikova V, Schweighofer N, Pitters E, Wohlfarter K, et al. Molecular mechanism of terbinafine resistance in Saccharomyces cerevisiae. Antimicrob Agents Chemother. 2003;47:3890-900 pubmed
    ..The results strongly indicate that these regions are responsible for the interaction of yeast squalene epoxidase with terbinafine. ..
  31. Schnabl M, Oskolkova O, Holic R, Brezna B, Pichler H, Zágorsek M, et al. Subcellular localization of yeast Sec14 homologues and their involvement in regulation of phospholipid turnover. Eur J Biochem. 2003;270:3133-45 pubmed
    ..In summary, Sfh2p and Sfh4p are the Sec14p homologues with the most pronounced functional similarity to Sec14p, whereas the other Sfh proteins appear to be functionally less related to Sec14p. ..
  32. Pichler H, Gaigg B, Hrastnik C, Achleitner G, Kohlwein S, Zellnig G, et al. A subfraction of the yeast endoplasmic reticulum associates with the plasma membrane and has a high capacity to synthesize lipids. Eur J Biochem. 2001;268:2351-61 pubmed
    ..A possible role of PAM in the supply of lipids to the plasma membrane is discussed. ..
  33. Athenstaedt K, Daum G. Biosynthesis of phosphatidic acid in lipid particles and endoplasmic reticulum of Saccharomyces cerevisiae. J Bacteriol. 1997;179:7611-6 pubmed
    ..Thus, yeast contains at least two independent systems of phosphatidic acid biosynthesis. ..
  34. Reiner S, Micolod D, Zellnig G, Schneiter R. A genomewide screen reveals a role of mitochondria in anaerobic uptake of sterols in yeast. Mol Biol Cell. 2006;17:90-103 pubmed
  35. Gsell M, Mascher G, Schuiki I, Ploier B, Hrastnik C, Daum G. Transcriptional response to deletion of the phosphatidylserine decarboxylase Psd1p in the yeast Saccharomyces cerevisiae. PLoS ONE. 2013;8:e77380 pubmed publisher
    ..Only three mutants, namely ?gpm2, ?gph1 and ?rsb1, were affected in one of these parameters. The possible link of these mutations to PE deficiency and PSD1 deletion is discussed. ..
  36. Zweytick D, Leitner E, Kohlwein S, Yu C, Rothblatt J, Daum G. Contribution of Are1p and Are2p to steryl ester synthesis in the yeast Saccharomyces cerevisiae. Eur J Biochem. 2000;267:1075-82 pubmed