Experts and Doctors on coculture techniques in Vienna, Austria


Locale: Vienna, Austria
Topic: coculture techniques

Top Publications

  1. Dietersdorfer E, Cervero Aragó S, Sommer R, Kirschner A, Walochnik J. Optimized methods for Legionella pneumophila release from its Acanthamoeba hosts. BMC Microbiol. 2016;16:74 pubmed publisher
    ..The current results demonstrated that the recovery of L. pneumophila from water systems may be underestimated if protozoal membranes are not disrupted. ..
  2. Jürgens B, Hainz U, Fuchs D, Felzmann T, Heitger A. Interferon-gamma-triggered indoleamine 2,3-dioxygenase competence in human monocyte-derived dendritic cells induces regulatory activity in allogeneic T cells. Blood. 2009;114:3235-43 pubmed publisher
    ..Together, these findings suggest that IFN-gamma triggered IDO competence in human mDCs constitutes a critical factor for endowing allogeneic T cells with regulatory activity. ..
  3. Nguyen C, Huttary N, Atanasov A, Chatuphonprasert W, Brenner S, Fristiohady A, et al. Fenofibrate inhibits tumour intravasation by several independent mechanisms in a 3-dimensional co-culture model. Int J Oncol. 2017;50:1879-1888 pubmed publisher
    ..A combination with other FDA-approved drugs further improved this effect. Our new concept may lead to a novel therapy for cancer patients. ..
  4. Appelt Menzel A, Cubukova A, Günther K, Edenhofer F, Piontek J, Krause G, et al. Establishment of a Human Blood-Brain Barrier Co-culture Model Mimicking the Neurovascular Unit Using Induced Pluri- and Multipotent Stem Cells. Stem Cell Reports. 2017;8:894-906 pubmed publisher
    ..Drug permeability tests with reference substances were performed and confirmed the suitability of the models for drug transport studies. ..
  5. Collingro A, Poppert S, Heinz E, Schmitz Esser S, Essig A, Schweikert M, et al. Recovery of an environmental Chlamydia strain from activated sludge by co-cultivation with Acanthamoeba sp. Microbiology. 2005;151:301-9 pubmed
  6. Smole U, Balazs N, Hoffmann Sommergruber K, Radauer C, Hafner C, Wallner M, et al. Differential T-cell responses and allergen uptake after exposure of dendritic cells to the birch pollen allergens Bet v 1.0101, Bet v 1.0401 and Bet v 1.1001. Immunobiology. 2010;215:903-9 pubmed publisher
    ..These results provide evidence that DCs discriminate between allergens and highly related hypoallergens. This process may have an impact on the early phase of sensitisation. ..
  7. Stary G, Klein I, Bauer W, Koszik F, Reininger B, Kohlhofer S, et al. Glucocorticosteroids modify Langerhans cells to produce TGF-? and expand regulatory T cells. J Immunol. 2011;186:103-12 pubmed publisher
    ..Our data show that systemically applied GCSs endow LCs with Treg-promoting properties and thus shed new light on the mechanisms of GCS-mediated immunosuppression...
  8. Wagner B, Sibilia M. Methods to study MAP kinase signalling in the central nervous system. Methods Mol Biol. 2010;661:481-95 pubmed publisher
  9. Smole U, Wagner S, Balazs N, Radauer C, Bublin M, Allmaier G, et al. Bet v 1 and its homologous food allergen Api g 1 stimulate dendritic cells from birch pollen-allergic individuals to induce different Th-cell polarization. Allergy. 2010;65:1388-96 pubmed publisher
    ..Bet v 1 in contrast to homologous food allergens seems to possess distinct molecular features that enable it to condition DCs from BP-allergic donors to induce allergen-specific T-cell proliferation and Th2 polarization. ..

More Information


  1. McBride J, Jung T, de Vries J, Aversa G. IL-10 alters DC function via modulation of cell surface molecules resulting in impaired T-cell responses. Cell Immunol. 2002;215:162-72 pubmed
    ..Transwell and DC-derived plasma membrane experiments indicated that the capacity of IL-10-treated DC to induce T-cell unresponsiveness results from alterations in the cell surface molecules rather than modulation of cytokine secretion. ..
  2. Mullauer L, Mosberger I, Grusch M, Rudas M, Chott A. Fas ligand is expressed in normal breast epithelial cells and is frequently up-regulated in breast cancer. J Pathol. 2000;190:20-30 pubmed
    ..These results suggest that increased expression of FasL may confer an advantage on breast cancer cells, possibly by eliminating tumour-infiltrating immune cells, and/or by facilitating tissue destruction during invasion. ..
  3. Kerjaschki D, Bago Horvath Z, Rudas M, Sexl V, Schneckenleithner C, Wolbank S, et al. Lipoxygenase mediates invasion of intrametastatic lymphatic vessels and propagates lymph node metastasis of human mammary carcinoma xenografts in mouse. J Clin Invest. 2011;121:2000-12 pubmed publisher
    ..These results provide evidence that lipoxygenase serves as a mediator of tumor cell invasion into lymphatic vessels and formation of lymph node metastasis in ductal mammary carcinomas. ..
  4. Sallmann E, Reininger B, Brandt S, Duschek N, Hoflehner E, Garner Spitzer E, et al. High-affinity IgE receptors on dendritic cells exacerbate Th2-dependent inflammation. J Immunol. 2011;187:164-71 pubmed publisher
    ..Thus, Fc?RI on DCs drives the cascade of pathogenic reactions linking the initial allergen capture by IgE with subsequent Th2-dominated T cell responses and the development of late-phase allergic tissue inflammation. ..
  5. Pfeifer S, Schreder M, Bolomsky A, Graffi S, Fuchs D, Sahota S, et al. Induction of indoleamine-2,3 dioxygenase in bone marrow stromal cells inhibits myeloma cell growth. J Cancer Res Clin Oncol. 2012;138:1821-30 pubmed publisher
  6. Ganas P, Liebhart D, Glösmann M, Hess C, Hess M. Escherichia coli strongly supports the growth of Histomonas meleagridis, in a monoxenic culture, without influence on its pathogenicity. Int J Parasitol. 2012;42:893-901 pubmed publisher
    ..Consequently, attenuation depends not on the bacteria in the culture but on the in vitro passages. Finally, the experiments provided evidence that the infection of turkeys with H. meleagridis enabled infection of the liver with E. coli...
  7. Sharma R, Fischer M, Bauer J, Felts P, Smith K, Misu T, et al. Inflammation induced by innate immunity in the central nervous system leads to primary astrocyte dysfunction followed by demyelination. Acta Neuropathol. 2010;120:223-36 pubmed publisher
    ..Similar structural abnormalities were also seen in a subset of active lesions in multiple sclerosis. Our studies suggest that astrocyte injury may be an important early step in the cascade of lesion formation in brain inflammation. ..
  8. Höflinger S, Kesavan K, Fuxa M, Hutter C, Heavey B, Radtke F, et al. Analysis of Notch1 function by in vitro T cell differentiation of Pax5 mutant lymphoid progenitors. J Immunol. 2004;173:3935-44 pubmed
    ..These data suggest that Notch1 acts upstream of GATA3 and Tcf1 in early T cell development and regulates Vbeta-DJbeta rearrangements by controlling the chromatin accessibility of Vbeta genes at the TCRbeta locus. ..