Experts and Doctors on testis in Melbourne, Victoria, Australia


Locale: Melbourne, Victoria, Australia
Topic: testis

Top Publications

  1. Siddall N, McLaughlin E, Marriner N, Hime G. The RNA-binding protein Musashi is required intrinsically to maintain stem cell identity. Proc Natl Acad Sci U S A. 2006;103:8402-7 pubmed
    ..We describe the complementary expression patterns of the murine Msi paralogues Msi1 and Msi2 during spermatogenesis, which support the idea of distinct, evolutionarily conserved roles of Msi. ..
  2. Takakubo F, Dahl H. The expression pattern of the pyruvate dehydrogenase E1 alpha subunit genes during spermatogenesis in adult mouse. Exp Cell Res. 1992;199:39-49 pubmed
  3. Graves J. The origin and function of the mammalian Y chromosome and Y-borne genes--an evolving understanding. Bioessays. 1995;17:311-20 pubmed
    ..Although the gonadogenesis pathway is highly conserved in vertebrates, its control has probably changed radically and rapidly in vertebrate--even mammalian--evolution. ..
  4. Pham S, Hong M, Teague J, Hutson J. Is the testis intraperitoneal?. Pediatr Surg Int. 2005;21:231-9 pubmed
    ..The apparent discrepancy between the adult testis being intraperitoneal or extraperitoneal is likely to result from differences in the relative size of the tunica vaginalis between infant boys and elderly men. ..
  5. Davis I, Chen W, Jackson H, Parente P, Shackleton M, Hopkins W, et al. Recombinant NY-ESO-1 protein with ISCOMATRIX adjuvant induces broad integrated antibody and CD4(+) and CD8(+) T cell responses in humans. Proc Natl Acad Sci U S A. 2004;101:10697-702 pubmed
    ..In an unplanned analysis, vaccinated patients appeared to have superior clinical outcomes to those treated with placebo or protein alone. The vaccine is safe and highly potent immunologically. ..
  6. Wreford N, Rajendra Kumar T, Matzuk M, de Kretser D. Analysis of the testicular phenotype of the follicle-stimulating hormone beta-subunit knockout and the activin type II receptor knockout mice by stereological analysis. Endocrinology. 2001;142:2916-20 pubmed
    ..We conclude that the defects in FSH beta-subunit gene knockout and ActRIIA knockout mice are related to diminished FSH action on both Sertoli cell proliferation and the capacity of Sertoli cells to nurture germ cells. ..
  7. O Bryan M, Takada S, Kennedy C, Scott G, Harada S, Ray M, et al. Sox8 is a critical regulator of adult Sertoli cell function and male fertility. Dev Biol. 2008;316:359-70 pubmed publisher
    ..These data show that SOX8 is a critical regulator of adult Sertoli cell function and is required for both its cytoarchitectural and paracrine interactions with germ cells. ..
  8. Knower K, Kelly S, Ludbrook L, Bagheri Fam S, Sim H, Bernard P, et al. Failure of SOX9 regulation in 46XY disorders of sex development with SRY, SOX9 and SF1 mutations. PLoS ONE. 2011;6:e17751 pubmed publisher
    ..We demonstrate how three human sex-determining factors are likely to function during gonadal development around SOX9 as a hub gene, with different genetic causes of 46,XY DSD due a common failure to upregulate SOX9 transcription. ..
  9. Hogarth C, Itman C, Jans D, Loveland K. Regulated nucleocytoplasmic transport in spermatogenesis: a driver of cellular differentiation?. Bioessays. 2005;27:1011-25 pubmed

More Information


  1. Nation T, Buraundi S, Balic A, Farmer P, Newgreen D, Southwell B, et al. The effect of flutamide on expression of androgen and estrogen receptors in the gubernaculum and surrounding structures during testicular descent. J Pediatr Surg. 2011;46:2358-62 pubmed publisher
    ..These results suggest that the IFP could be the site of androgenic action controlling gubernacular development. ..
  2. Kennedy C, Koopman P, Mishina Y, O Bryan M. Sox8 and Sertoli-cell function. Ann N Y Acad Sci. 2007;1120:104-13 pubmed
    ..We conclude that SOX8 transactivated genes have key roles in adult Sertoli-cell function and its elimination results in progressive male infertility. ..
  3. Fitzgerald J, Hutchison W, Dahl H. Isolation and characterisation of the mouse pyruvate dehydrogenase E1 alpha genes. Biochim Biophys Acta. 1992;1131:83-90 pubmed
  4. Speed C, Matzaris M, Bird P, Mitchell C. Tissue distribution and intracellular localisation of the 75-kDa inositol polyphosphate 5-phosphatase. Eur J Biochem. 1995;234:216-24 pubmed
    ..This study demonstrates the tissue distribution and intracellular location of the 75-kDa 5-phosphatase and reveals a novel location for an enzyme involved in phosphatidylinositol turnover. ..
  5. Hu Y, Yu H, Pask A, O Brien D, Shaw G, Renfree M. A-kinase anchoring protein 4 has a conserved role in mammalian spermatogenesis. Reproduction. 2009;137:645-53 pubmed publisher
    ..This is consistent with its expression in other mammals. Thus, AKAP4 appears to have a conserved role in spermatogenesis for at least the last 166 million years of mammalian evolution...
  6. Albiston A, Obeyesekere V, Smith R, Krozowski Z. Cloning and tissue distribution of the human 11 beta-hydroxysteroid dehydrogenase type 2 enzyme. Mol Cell Endocrinol. 1994;105:R11-7 pubmed
    ..These data suggest that 11 beta HSD2 plays an important role in modulating mineralocorticoid and glucocorticoid receptor occupancy by glucocorticoids. ..
  7. Itman C, Whiley P, Zhou W, Meistrich M, Sahin Z, Loveland K. Regulated production of SnoN2 is a feature of testicular differentiation. Microsc Res Tech. 2009;72:833-44 pubmed publisher
  8. Dahl H, Brown R, Hutchison W, Maragos C, Brown G. A testis-specific form of the human pyruvate dehydrogenase E1 alpha subunit is coded for by an intronless gene on chromosome 4. Genomics. 1990;8:225-32 pubmed
    ..The transcribed region of this gene is identical to the testis-specific cDNA sequence. It completely lacks introns and possesses characteristics of a functional processed gene. ..
  9. Lambeth L, Ayers K, Cutting A, Doran T, Sinclair A, Smith C. Anti-Müllerian Hormone Is Required for Chicken Embryonic Urogenital System Growth but Not Sexual Differentiation. Biol Reprod. 2015;93:138 pubmed publisher
    ..These data suggest that although AMH does not directly contribute to testicular or ovarian differentiation, it is required in a sex-independent manner for proper cell proliferation and urogenital system growth. ..
  10. Itman C, Wong C, Hunyadi B, Ernst M, Jans D, Loveland K. Smad3 dosage determines androgen responsiveness and sets the pace of postnatal testis development. Endocrinology. 2011;152:2076-89 pubmed publisher
  11. Smith C, McClive P, Sinclair A. Temporal and spatial expression profile of the novel armadillo-related gene, Alex2, during testicular differentiation in the mouse embryo. Dev Dyn. 2005;233:188-93 pubmed
    ..In the developing testis, its expression profile suggests that Alex2 has a role in specification or development of the interstitial cell lineage. ..
  12. Czech D, Lee J, Sim H, Parish C, Vilain E, Harley V. The human testis-determining factor SRY localizes in midbrain dopamine neurons and regulates multiple components of catecholamine synthesis and metabolism. J Neurochem. 2012;122:260-71 pubmed publisher
    ..This ancillary genetic mechanism might contribute to gender bias in fight-flight behaviours in men or their increased susceptibility to dopamine disorders, such as Parkinson's disease and schizophrenia. ..
  13. Xu H, Beasley M, Warren W, van der Horst G, McKay M. Absence of mouse REC8 cohesin promotes synapsis of sister chromatids in meiosis. Dev Cell. 2005;8:949-61 pubmed
    ..Unexpectedly, Rec8 null mice are born in sub-Mendelian frequencies and fail to thrive. These findings illuminate hitherto unknown REC8 functions in chromosome dynamics during mammalian meiosis and possibly in somatic development. ..
  14. Harisis G, Chen N, Farmer P, Bodemer D, Li R, Sourial M, et al. Wnt signalling in testicular descent: a candidate mechanism for cryptorchidism in Robinow syndrome. J Pediatr Surg. 2013;48:1573-7 pubmed publisher
    ..Human mutations in Wnt-5a or Ror2 could prevent early gubernacular growth, as suggested by undescended testes in 70% of patients with Robinow Syndrome. ..
  15. Hedger M, Winnall W. Regulation of activin and inhibin in the adult testis and the evidence for functional roles in spermatogenesis and immunoregulation. Mol Cell Endocrinol. 2012;359:30-42 pubmed publisher
    ..Consequently, elevated activin A production within the testis during inflammation and infection may contribute to spermatogenic failure, fibrosis and testicular damage. ..
  16. Loveland K, Bakker M, Meehan T, Christy E, von Schönfeldt V, Drummond A, et al. Expression of Bambi is widespread in juvenile and adult rat tissues and is regulated in male germ cells. Endocrinology. 2003;144:4180-6 pubmed
    ..Regulated Bambi mRNA synthesis may contribute to TGF beta superfamily signaling modulation in several organs, as suggested by its discrete expression switch in male germ cells. ..
  17. Kubota Y, Nef S, Farmer P, Temelcos C, Parada L, Hutson J. Leydig insulin-like hormone, gubernacular development and testicular descent. J Urol. 2001;165:1673-5 pubmed
    ..Insl3 mutants show feminized gubernaculum with deficient mesenchymal core. Insl3 appears to have some role in the gubernacular swelling reaction in mice. ..
  18. Li R, Zhang J, CHURCHILL J, Sourial M, Southwell B, Hutson J. Is matrix metalloproteinase required in postnatal testicular tubules for germ cell maturation?. J Pediatr Surg. 2012;47:1724-9 pubmed publisher
    ..Increased knowledge about germ cell development may aid efforts to improve fertility in cryptorchidism. ..
  19. Szczepny A, Hogarth C, Young J, Loveland K. Identification of Hedgehog signaling outcomes in mouse testis development using a hanging drop-culture system. Biol Reprod. 2009;80:258-63 pubmed publisher
    ..These novel results identify Hh target genes in the testis and demonstrate this signaling pathway likely affects cell survival and differentiation in the context of normal adult testis. ..
  20. Gao Y, Short R. Fertility control in laboratory rats and mice after feeding with the antigestagen RU486. J Reprod Fertil. 1994;101:477-81 pubmed
    ..The treatment period was therefore reduced to 3 days every 18 days and no more litters were produced and none of the treated females was pregnant at autopsy.(ABSTRACT TRUNCATED AT 250 WORDS) ..
  21. Smith C, Roeszler K, Ohnesorg T, Cummins D, Farlie P, Doran T, et al. The avian Z-linked gene DMRT1 is required for male sex determination in the chicken. Nature. 2009;461:267-71 pubmed publisher
    ..These results indicate that DMRT1 is required for testis determination in the chicken. Our data support the Z dosage hypothesis for avian sex determination. ..
  22. Itman C, Loveland K. SMAD expression in the testis: an insight into BMP regulation of spermatogenesis. Dev Dyn. 2008;237:97-111 pubmed
    ..Based on these findings, we extrapolate these data to interpret BMP mutant testis phenotypes in terms of SMAD availability for signal transduction. ..
  23. Nation T, Buraundi S, Farmer P, Balic A, Newgreen D, Southwell B, et al. Development of the gubernaculum during testicular descent in the rat. Anat Rec (Hoboken). 2011;294:1249-60 pubmed publisher
    ..Finally, gubernacular eversion at birth brings the cranial proliferative zone to the external distal tip, enabling gubernacular elongation similar to a limb bud. ..
  24. Yu H, Lindsay J, Feng Z, Frankenberg S, Hu Y, Carone D, et al. Evolution of coding and non-coding genes in HOX clusters of a marsupial. BMC Genomics. 2012;13:251 pubmed publisher
    ..The sequencing of the kangaroo genome provides an opportunity to use comparative analyses to compare the HOX clusters of a mammal with a distinct body plan to those of other mammals...
  25. Hutson J, Balic A, Nation T, Southwell B. Cryptorchidism. Semin Pediatr Surg. 2010;19:215-24 pubmed publisher
    ..Further research into the cause and management of undescended testes is necessary...
  26. Nightingale S, Western P, Hutson J. The migrating gubernaculum grows like a "limb bud". J Pediatr Surg. 2008;43:387-90 pubmed publisher
    ..Hox genes and Fgfs may be fruitful areas of research to unravel the molecular control of gubernacular migration during testicular descent. ..
  27. Sarraj M, McClive P, Szczepny A, Daggag H, Loveland K, Sinclair A. Expression of Wsb2 in the developing and adult mouse testis. Reproduction. 2007;133:753-61 pubmed
  28. Gibbs G, Bianco D, Jamsai D, Herlihy A, Ristevski S, Aitken R, et al. Cysteine-rich secretory protein 2 binds to mitogen-activated protein kinase kinase kinase 11 in mouse sperm. Biol Reprod. 2007;77:108-14 pubmed
    ..These data raise the possibility that CRISP2 is a MAP3K11-modifying protein or, alternatively, that MAP3K11 acts to phosphorylate CRISP2 during acrosome development. ..
  29. Phelan D, Loveland K, Devereux L, Dorow D. Expression of mixed lineage kinase 2 in germ cells of the testis. Mol Reprod Dev. 1999;52:135-40 pubmed
    ..Furthermore, the finding of MLK2 protein in the nucleus of spermatocytes and round spermatids indicates a role for MLK2 in regulation of nuclear events specific to germ cell development. ..
  30. Iannello R, Dahl H. Transcriptional expression of a testis-specific variant of the mouse pyruvate dehydrogenase E1 alpha subunit. Biol Reprod. 1992;47:48-58 pubmed
    ..We conclude from these results that expression of the testis-specific E1 alpha is initially expressed during the meiotic prophase stage of spermatogenesis and not under any apparent post-transcriptional regulation. ..
  31. Nagraj S, Seah G, Farmer P, Davies B, Southwell B, Lewis A, et al. The development and anatomy of the gubernaculum in Hoxa11 knockout mice. J Pediatr Surg. 2011;46:387-92 pubmed publisher
    ..Hoxa11 mutations may well contribute to failure of gubernacular migration in boys with cryptorchidism. ..
  32. Gustin S, Western P, McClive P, Harley V, Koopman P, Sinclair A. Testis development, fertility, and survival in Ethanolamine kinase 2-deficient mice. Endocrinology. 2008;149:6176-86 pubmed publisher
    ..Expression of EGFP in this mouse model enabled the isolation of gonad cell populations, providing a useful resource from which to obtain relatively pure early steroidogenic cells for further studies. ..
  33. Harnaen E, Na A, Shenker N, Sourial M, Farmer P, Southwell B, et al. The anatomy of the cremaster muscle during inguinoscrotal testicular descent in the rat. J Pediatr Surg. 2007;42:1982-7 pubmed
    ..The cremaster muscle appears to differentiate from the gubernacular tip during elongation to the scrotum, and requires intact sensory innervation and androgen. ..
  34. SANDERS N, Buraundi S, Balic A, Southwell B, Hutson J. Cremaster muscle myogenesis in the tip of the rat gubernaculum supports active gubernacular elongation during inguinoscrotal testicular descent. J Urol. 2011;186:1606-13 pubmed publisher
    ..The cremaster may originate from abdominal muscles during gubernacular eversion or alternatively it may develop inside the gubernaculum. We studied cremaster myogenesis to determine how it develops...
  35. Chen N, Harisis G, Farmer P, Buraundi S, Sourial M, Southwell B, et al. Gone with the Wnt: the canonical Wnt signaling axis is present and androgen dependent in the rodent gubernaculum. J Pediatr Surg. 2011;46:2363-9 pubmed publisher
    ..As androgen is required for inguinoscrotal descent, downstream Wnt signaling may control initial gubernacular remodeling. Defects in this complex molecular process may play a role in cryptorchidism. ..
  36. Ludbrook L, Bernard P, Bagheri Fam S, Ryan J, Sekido R, Wilhelm D, et al. Excess DAX1 leads to XY ovotesticular disorder of sex development (DSD) in mice by inhibiting steroidogenic factor-1 (SF1) activation of the testis enhancer of SRY-box-9 (Sox9). Endocrinology. 2012;153:1948-58 pubmed publisher
    ..In cultured cells, increasing levels of DAX1 antagonized SF1-, SF1/SRY-, and SF1/SOX9-mediated activation of TES, due to reduced binding of SF1 to TES, providing a likely mechanism for DSS. ..
  37. Smith C, McClive P, Hudson Q, Sinclair A. Male-specific cell migration into the developing gonad is a conserved process involving PDGF signalling. Dev Biol. 2005;284:337-50 pubmed
    ..Taken together, these studies indicate that a key cellular event in gonadal sex differentiation is conserved among higher vertebrates, that it involves PDGF signalling, and that in mammals is an indirect effect of Sry expression. ..
  38. Donaldson K, Tong S, Washburn T, Lubahn D, Eddy E, Hutson J, et al. Morphometric study of the gubernaculum in male estrogen receptor mutant mice. J Androl. 1996;17:91-5 pubmed
    ..This study demonstrates that estrogen has a previously unknown role in masculine sexual development of the gubernaculum and the structures derived from it, such as the cremaster muscle. ..
  39. Ke F, Bouillet P, Kaufmann T, Strasser A, Kerr J, Voss A. Consequences of the combined loss of BOK and BAK or BOK and BAX. Cell Death Dis. 2013;4:e650 pubmed publisher
  40. Iannello R, Kola I, Dahl H. Temporal and tissue-specific interactions involving novel transcription factors and the proximal promoter of the mouse Pdha-2 gene. J Biol Chem. 1993;268:22581-90 pubmed
    ..Our results suggest that 1) the MEP-2 binding factors are temporally regulated during spermatogenesis, and 2) interactions involving these factors with the MEP-2 cis-element may be important for modulating Pdha-2 expression. ..