Experts and Doctors on saccharomyces cerevisiae in Sydney, New South Wales, Australia


Locale: Sydney, New South Wales, Australia
Topic: saccharomyces cerevisiae

Top Publications

  1. Drakulic T, Temple M, Guido R, Jarolim S, Breitenbach M, Attfield P, et al. Involvement of oxidative stress response genes in redox homeostasis, the level of reactive oxygen species, and ageing in Saccharomyces cerevisiae. FEMS Yeast Res. 2005;5:1215-28 pubmed
    ..Heterogeneity was detected in populations of strains with compromised anti-oxidant defences, and as cells aged they shifted from one cell type with low ROS content to another with much higher intracellular ROS. ..
  2. Ayer A, Sanwald J, Pillay B, Meyer A, Perrone G, Dawes I. Distinct redox regulation in sub-cellular compartments in response to various stress conditions in Saccharomyces cerevisiae. PLoS ONE. 2013;8:e65240 pubmed publisher
    ..Redox state is distinctly regulated in organelles and data presented challenge the notion that perturbation of redox state is central in the response to many stress conditions. ..
  3. Robertson G, Whalley J. Evolution of the herpes thymidine kinase: identification and comparison of the equine herpesvirus 1 thymidine kinase gene reveals similarity to a cell-encoded thymidylate kinase. Nucleic Acids Res. 1988;16:11303-17 pubmed
    ..The implications for the evolution of enzyme activities within a pathway of nucleotide metabolism are discussed. ..
  4. Neumann A, Reddel R. Telomere maintenance and cancer -- look, no telomerase. Nat Rev Cancer. 2002;2:879-84 pubmed
  5. Böcking T, Barrow K, Netting A, Chilcott T, Coster H, Hofer M. Effects of singlet oxygen on membrane sterols in the yeast Saccharomyces cerevisiae. Eur J Biochem. 2000;267:1607-18 pubmed
    ..Subsequent permeation of photosensitizer through the plasma membrane into the cell leads to events including impairment of mitochondrial function and cell inactivation. ..
  6. Grant C, MacIver F, Dawes I. Glutathione synthetase is dispensable for growth under both normal and oxidative stress conditions in the yeast Saccharomyces cerevisiae due to an accumulation of the dipeptide gamma-glutamylcysteine. Mol Biol Cell. 1997;8:1699-707 pubmed
    ..We suggest that this function may be the detoxification of harmful intermediates that are generated during normal cellular metabolism. ..
  7. Lee J, Straffon M, Jang T, Higgins V, Grant C, Dawes I. The essential and ancillary role of glutathione in Saccharomyces cerevisiae analysed using a grande gsh1 disruptant strain. FEMS Yeast Res. 2001;1:57-65 pubmed
    ..Interestingly, the gsh1 sgr1 mutant generated petites at a lower rate than the gsh1 mutant. Thus, it is suggested that the essential role of GSH is involved in the maintenance of the mitochondrial genome. ..
  8. Ozsarac N, Bhattacharyya M, Dawes I, Clancy M. The SPR3 gene encodes a sporulation-specific homologue of the yeast CDC3/10/11/12 family of bud neck microfilaments and is regulated by ABFI. Gene. 1995;164:157-62 pubmed
  9. Low J, Im H, Erce M, Hart Smith G, Snyder M, Wilkins M. Protein substrates of the arginine methyltransferase Hmt1 identified by proteome arrays. Proteomics. 2016;16:465-76 pubmed publisher
    ..These results confirm arginine methylation as a widespread modification and Hmt1 as the major arginine methyltransferase in the S. cerevisiae cell. ..

More Information


  1. Lam Y, Stocker R, Dawes I. The lipophilic antioxidants alpha-tocopherol and coenzyme Q10 reduce the replicative lifespan of Saccharomyces cerevisiae. Free Radic Biol Med. 2010;49:237-44 pubmed publisher
    ..Supplementation with either coenzyme Q(10) alone, or in combination with alpha-tocopherol also led to a reduction in yeast replicative lifespan. This study highlights a potential pro-oxidant action of antioxidants. ..
  2. Park J, Collinson E, Grant C, Dawes I. Rom2p, the Rho1 GTP/GDP exchange factor of Saccharomyces cerevisiae, can mediate stress responses via the Ras-cAMP pathway. J Biol Chem. 2005;280:2529-35 pubmed
  3. Day R, Higgins V, Rogers P, Dawes I. Characterization of the putative maltose transporters encoded by YDL247w and YJR160c. Yeast. 2002;19:1015-27 pubmed
  4. Smith J, Jamie J, Guillemin G. Kynurenine-3-monooxygenase: a review of structure, mechanism, and inhibitors. Drug Discov Today. 2016;21:315-24 pubmed publisher
  5. Aung Htut M, Lam Y, Lim Y, Rinnerthaler M, Gelling C, Yang H, et al. Maintenance of mitochondrial morphology by autophagy and its role in high glucose effects on chronological lifespan of Saccharomyces cerevisiae. Oxid Med Cell Longev. 2013;2013:636287 pubmed publisher
    ..These data indicate that chronological lifespan seems correlated with mitochondrial morphology of yeast cells and that both phenotypes can be influenced by factors from conditioned medium of cultures grown in low glucose medium. ..
  6. Park J, Grant C, Dawes I. The high-affinity cAMP phosphodiesterase of Saccharomyces cerevisiae is the major determinant of cAMP levels in stationary phase: involvement of different branches of the Ras-cyclic AMP pathway in stress responses. Biochem Biophys Res Commun. 2005;327:311-9 pubmed
  7. Gelling C, Piper M, Hong S, Kornfeld G, Dawes I. Identification of a novel one-carbon metabolism regulon in Saccharomyces cerevisiae. J Biol Chem. 2004;279:7072-81 pubmed
    ..The one-carbon response is distinct from the Bas1p purine biosynthesis regulon and thus represents the first example of transcriptional regulation in response to activated one-carbon status. ..
  8. Perdomo J, Holmes M, Chong B, Crossley M. Eos and pegasus, two members of the Ikaros family of proteins with distinct DNA binding activities. J Biol Chem. 2000;275:38347-54 pubmed
    ..Our results suggest that these proteins may associate with previously described Ikaros family proteins in lymphoid cells and play additional roles in other tissues. ..
  9. Jagtap U, Jadhav J, Bapat V, Pretorius I. Synthetic biology stretching the realms of possibility in wine yeast research. Int J Food Microbiol. 2017;252:24-34 pubmed publisher
    ..0 (or Sc2.0) project, aimed at the synthesis of the entire genome of a laboratory strain of S. cerevisiae, might accelerate the design of improved wine yeasts. ..
  10. Ta M, Kapterian T, Fei W, Du X, Brown A, Dawes I, et al. Accumulation of squalene is associated with the clustering of lipid droplets. FEBS J. 2012;279:4231-44 pubmed publisher
    ..These results indicate that the level of squalene may affect the growth and distribution of lipid droplets. ..
  11. Sobti M, Cubeddu L, Haynes P, Mabbutt B. Engineered rings of mixed yeast Lsm proteins show differential interactions with translation factors and U-rich RNA. Biochemistry. 2010;49:2335-45 pubmed publisher
    ..Our findings suggest Lsm1 and/or Lsm4 can interact with translationally active mRNA. ..
  12. Ng C, Tan S, Perrone G, Thorpe G, Higgins V, Dawes I. Adaptation to hydrogen peroxide in Saccharomyces cerevisiae: the role of NADPH-generating systems and the SKN7 transcription factor. Free Radic Biol Med. 2008;44:1131-45 pubmed publisher
    ..These mutants overproduced reduced glutathione (GSH) but maintained normal cellular redox homeostasis. This overproduction of GSH was not regulated at transcription of the gene encoding gamma-glutamylcysteine synthetase. ..
  13. Park J, Grant C, Davies M, Dawes I. The cytoplasmic Cu,Zn superoxide dismutase of saccharomyces cerevisiae is required for resistance to freeze-thaw stress. Generation of free radicals during freezing and thawing. J Biol Chem. 1998;273:22921-8 pubmed
  14. Hong S, Piper M, Sinclair D, Dawes I. Control of expression of one-carbon metabolism genes of Saccharomyces cerevisiae is mediated by a tetrahydrofolate-responsive protein binding to a glycine regulatory region including a core 5'-CTTCTT-3' motif. J Biol Chem. 1999;274:10523-32 pubmed
    ..Tetrahydrofolate or a derivative may act as a ligand for the transcription factor controlling expression of one-carbon metabolism genes. ..
  15. Draculic T, Dawes I, Grant C. A single glutaredoxin or thioredoxin gene is essential for viability in the yeast Saccharomyces cerevisiae. Mol Microbiol. 2000;36:1167-74 pubmed
  16. Lyons R, Deane R, Lynch D, Ye Z, Sanderson G, Eyre H, et al. Identification of a novel human tankyrase through its interaction with the adaptor protein Grb14. J Biol Chem. 2001;276:17172-80 pubmed
    ..This study supports a role for the tankyrases in cytoplasmic signal transduction pathways and suggests that vesicle trafficking may be involved in the subcellular localization or signaling function of Grb14. ..
  17. Chiu J, Tactacan C, Tan S, Lin R, Wouters M, Dawes I. Cell cycle sensing of oxidative stress in Saccharomyces cerevisiae by oxidation of a specific cysteine residue in the transcription factor Swi6p. J Biol Chem. 2011;286:5204-14 pubmed publisher
  18. Wong J, Ho S, Hogg P. Disulfide bond acquisition through eukaryotic protein evolution. Mol Biol Evol. 2011;28:327-34 pubmed publisher
    ..Finally, we show that the acquisition of the functionally relevant disulfide bond in domain 2 of the CD4 protein occurred independently in primates and rodents. ..
  19. Fei W, Shui G, Zhang Y, Krahmer N, Ferguson C, Kapterian T, et al. A role for phosphatidic acid in the formation of "supersized" lipid droplets. PLoS Genet. 2011;7:e1002201 pubmed publisher
    ..In summary, our results provide important insights into how the size of LDs is determined and identify novel gene products that regulate phospholipid metabolism. ..
  20. Alic N, Higgins V, Dawes I. Identification of a Saccharomyces cerevisiae gene that is required for G1 arrest in response to the lipid oxidation product linoleic acid hydroperoxide. Mol Biol Cell. 2001;12:1801-10 pubmed
    ..Identification of OCA1 establishes cell cycle arrest as an actively regulated response to oxidative stress and will enable further elucidation of oxidative stress-responsive signaling pathways in yeast. ..
  21. Biagini G, Park J, Lloyd D, Edwards M. The antioxidant potential of pyruvate in the amitochondriate diplomonads Giardia intestinalis and Hexamita inflata. Microbiology. 2001;147:3359-65 pubmed
    ..intestinalis and H. inflata, pyruvate exerts antioxidant activity at physiological levels, and (ii) it is the absence of a respiratory chain in the diplomonads which facilitates the observed antioxidant activity...
  22. Coe J, Murray L, Dawes I. Identification of a sporulation-specific promoter regulating divergent transcription of two novel sporulation genes in Saccharomyces cerevisiae. Mol Gen Genet. 1994;244:661-72 pubmed
    ..Therefore these genes belong to the class of late sporulation-specific genes that are sequentially activated during the process of meiosis and spore formation. ..
  23. Naidoo N, Harrop S, Sobti M, Haynes P, Szymczyna B, Williamson J, et al. Crystal structure of Lsm3 octamer from Saccharomyces cerevisiae: implications for Lsm ring organisation and recruitment. J Mol Biol. 2008;377:1357-71 pubmed publisher
    ..Our coordinates also provide updated homology models for the active Lsm[1-7] and Lsm[2-8] heptameric rings. ..
  24. Collinson L, Dawes I. Isolation, characterization and overexpression of the yeast gene, GLR1, encoding glutathione reductase. Gene. 1995;156:123-7 pubmed
    ..8% aa identity. Yeast cells transformed with a multicopy plasmid containing the genomic clone overproduced GR activity sixfold. GLR1 was found not to be an essential gene. ..
  25. Issa L, Leong G, Barry J, Sutherland R, Eisman J. Glucocorticoid receptor-interacting protein-1 and receptor-associated coactivator-3 differentially interact with the vitamin D receptor (VDR) and regulate VDR-retinoid X receptor transcriptional cross-talk. Endocrinology. 2001;142:1606-15 pubmed
    ..These data suggest different coactivators regulate VDR function via distinct mechanisms and support the hypothesis that the VDR recruits different coactivators depending on specific gene and cellular contexts. ..
  26. Luikenhuis S, Perrone G, Dawes I, Grant C. The yeast Saccharomyces cerevisiae contains two glutaredoxin genes that are required for protection against reactive oxygen species. Mol Biol Cell. 1998;9:1081-91 pubmed
    ..Thus, Grx1 and Grx2 function differently in the cell, and we suggest that glutaredoxins may act as one of the primary defenses against mixed disulfides formed following oxidative damage to proteins. ..
  27. Piper M, Hong S, Ball G, Dawes I. Regulation of the balance of one-carbon metabolism in Saccharomyces cerevisiae. J Biol Chem. 2000;275:30987-95 pubmed
    ..When glycine is in excess, 5, 10-CH(2)-H(4)folate is decreased, and the regulation loop shifts the balance of generation of one-carbon units into the mitochondrion. ..
  28. Hart Smith G, Yagoub D, Tay A, Pickford R, Wilkins M. Large Scale Mass Spectrometry-based Identifications of Enzyme-mediated Protein Methylation Are Subject to High False Discovery Rates. Mol Cell Proteomics. 2016;15:989-1006 pubmed publisher
    ..Data are available via ProteomeXchange with the data identifier PXD002857. ..
  29. Richardson D. The biogenesis of [Fe-S] clusters: the role of the unorthodox ABC ATPase, SufC, and the wider implications for understanding iron metabolism. Redox Rep. 2003;8:125-7 pubmed
  30. Collinson E, Wimmer Kleikamp S, Gerega S, Yang Y, Parish C, Dawes I, et al. The yeast homolog of heme oxygenase-1 affords cellular antioxidant protection via the transcriptional regulation of known antioxidant genes. J Biol Chem. 2011;286:2205-14 pubmed publisher
    ..They indicate much broader functions for HO-1, the unraveling of which may help explain the multiple biological responses reported in animals as a result of altered HO-1 expression. ..
  31. Pang C, Gasteiger E, Wilkins M. Identification of arginine- and lysine-methylation in the proteome of Saccharomyces cerevisiae and its functional implications. BMC Genomics. 2010;11:92 pubmed publisher
    ..This study suggests protein methylation to be quite widespread, albeit associated with specific functions. Large-scale tandem mass spectroscopy analyses will help to further confirm the modifications reported here. ..
  32. Tan S, Teo M, Lam Y, Dawes I, Perrone G. Cu, Zn superoxide dismutase and NADP(H) homeostasis are required for tolerance of endoplasmic reticulum stress in Saccharomyces cerevisiae. Mol Biol Cell. 2009;20:1493-508 pubmed publisher
    ..These data indicate an important role for SOD and cellular NADP(H) in cell survival during ER stress, and it is proposed that accumulation of superoxide affects NADP(H) homeostasis, leading to reduced UPR induction during ER stress. ..
  33. Beilharz T, Preiss T. Widespread use of poly(A) tail length control to accentuate expression of the yeast transcriptome. RNA. 2007;13:982-97 pubmed
    ..They further provide a molecular explanation for deadenylase function in the cell cycle and suggest additional cellular processes that depend on control of mRNA polyadenylation. ..
  34. Howell K, Cozzolino D, Bartowsky E, Fleet G, Henschke P. Metabolic profiling as a tool for revealing Saccharomyces interactions during wine fermentation. FEMS Yeast Res. 2006;6:91-101 pubmed
    ..This study provides a novel way to measure the population status of wine fermentations by metabolic footprinting. ..
  35. Grant C, MacIver F, Dawes I. Stationary-phase induction of GLR1 expression is mediated by the yAP-1 transcriptional regulatory protein in the yeast Saccharomyces cerevisiae. Mol Microbiol. 1996;22:739-46 pubmed
    ..In addition, strains lacking GLR or yAP-1 do not accumulate GSSG during stationary-phase growth, indicating that the cell possesses alternative means of preventing an accumulation of GSSG during stationary phase. ..
  36. Bryant N, James D. The Sec1p/Munc18 (SM) protein, Vps45p, cycles on and off membranes during vesicle transport. J Cell Biol. 2003;161:691-6 pubmed
    ..These data reveal that SM proteins cycle on and off membranes in a stage-specific manner during the vesicle transport reaction, and suggest that protein phosphorylation plays a key role in the regulation of this cycle. ..
  37. Ayer A, Tan S, Grant C, Meyer A, Dawes I, Perrone G. The critical role of glutathione in maintenance of the mitochondrial genome. Free Radic Biol Med. 2010;49:1956-68 pubmed publisher
    ..GSH may also modulate the redox environment of the IMS. The implications of altered GSH homeostasis for maintenance of mtDNA, compartmental redox, and the pathophysiology of certain diseases are discussed. ..
  38. Sinclair D, Hong S, Dawes I. Specific induction by glycine of the gene for the P-subunit of glycine decarboxylase from Saccharomyces cerevisiae. Mol Microbiol. 1996;19:611-23 pubmed
    ..Deletion analysis of the GCV2 promoter delimited the control region which contains putative regulatory sites for GCN4 and GLN3 transcription factors. ..
  39. Liachko I, Tanaka E, Cox K, Chung S, Yang L, Seher A, et al. Novel features of ARS selection in budding yeast Lachancea kluyveri. BMC Genomics. 2011;12:633 pubmed publisher
    ..Furthermore, we have developed new approaches for analyzing biologically functional DNA sequences with ill-defined motifs. ..
  40. Tan S, Greetham D, Raeth S, Grant C, Dawes I, Perrone G. The thioredoxin-thioredoxin reductase system can function in vivo as an alternative system to reduce oxidized glutathione in Saccharomyces cerevisiae. J Biol Chem. 2010;285:6118-26 pubmed publisher
    ..Collectively, these data indicate that the thioredoxin-thioredoxin reductase system can function as an alternative system to reduce GSSG in S. cerevisiae in vivo. ..