Experts and Doctors on kinetics in Canberra, Australian Capital Territory, Australia


Locale: Canberra, Australian Capital Territory, Australia
Topic: kinetics

Top Publications

  1. Lobigs M, Usha R, Nestorowicz A, Marshall I, Weir R, Dalgarno L. Host cell selection of Murray Valley encephalitis virus variants altered at an RGD sequence in the envelope protein and in mouse virulence. Virology. 1990;176:587-95 pubmed
    ..All five variants which were altered at Asp 390 were attenuated in 21-day-old mice following i.p. inoculation. We propose that the domain of E encompassing the RGD sequence is an important determinant of flavivirus pathogenicity. ..
  2. Sim A, White M, Denborough M. The effects of chlorbutol on skeletal muscle sarcoplasmic reticulum function in porcine malignant hyperpyrexia. Int J Biochem. 1987;19:827-30 pubmed
    ..2. Studies on isolated sarcoplasmic reticulum vesicles have shown that this effect of chlorbutol in MH is not due to a modification of Ca2+-transport mechanisms...
  3. Bernardo P, Brasch N, Chai C, Waring P. A novel redox mechanism for the glutathione-dependent reversible uptake of a fungal toxin in cells. J Biol Chem. 2003;278:46549-55 pubmed
    ..The toxin effluxes from the apoptotic cell exclusively in the oxidized form and can further enter and kill neighboring cells, thus acting in a pseudocatalytic way. ..
  4. Taylor M, Jackson C, Tattersall D, French N, Peat T, Newman J, et al. Identification and characterization of two families of F420 H2-dependent reductases from Mycobacteria that catalyse aflatoxin degradation. Mol Microbiol. 2010;78:561-75 pubmed publisher
  5. Alonso H, Blayney M, Beck J, Whitney S. Substrate-induced assembly of Methanococcoides burtonii D-ribulose-1,5-bisphosphate carboxylase/oxygenase dimers into decamers. J Biol Chem. 2009;284:33876-82 pubmed publisher
    ..5 microM). The catalytic and structural similarities of MbR to other archaeal Rubiscos contrast with its closer sequence homology to bacterial L(2) Rubisco, complicating its classification within the Rubisco superfamily...
  6. Neylon C, Brown S, Kralicek A, Miles C, Love C, Dixon N. Interaction of the Escherichia coli replication terminator protein (Tus) with DNA: a model derived from DNA-binding studies of mutant proteins by surface plasmon resonance. Biochemistry. 2000;39:11989-99 pubmed
    ..The kinetic and thermodynamic data suggest a model for Tus binding to TerB which involves an ordered series of events that include structural changes in the protein. ..
  7. Lam M, Hu W, Xiao C, Gillespie M, Jans D. Molecular dissection of the importin beta1-recognized nuclear targeting signal of parathyroid hormone-related protein. Biochem Biophys Res Commun. 2001;282:629-34 pubmed
    ..This study represents the first detailed examination of a modular, non-arginine-rich importin beta1-recognized nuclear targeting signal. ..
  8. Tetlow N, Board P. Functional polymorphism of human glutathione transferase A2. Pharmacogenetics. 2004;14:111-6 pubmed
    ..The position of this polymorphism in the active site and its effects on model substrates suggest that further investigation of its capacity to conjugate alkylating drugs is warranted. ..
  9. Hu W, Kemp B, Jans D. Kinetic properties of nuclear transport conferred by the retinoblastoma (Rb) NLS. J Cell Biochem. 2005;95:782-93 pubmed

More Information


  1. Alsharifi M, Lobigs M, Regner M, Lee E, Koskinen A, Mullbacher A. Type I interferons trigger systemic, partial lymphocyte activation in response to viral infection. J Immunol. 2005;175:4635-40 pubmed
    ..We conclude that virus-induced IFN-I release systemically affects most of the hosts T and B cells by triggering them rapidly and independently of Ag-reactivity into a semiactivated state...
  2. Mulcair M, Schaeffer P, Oakley A, Cross H, Neylon C, Hill T, et al. A molecular mousetrap determines polarity of termination of DNA replication in E. coli. Cell. 2006;125:1309-19 pubmed
    ..Lock formation specifically requires the cytosine residue, C(6). The crystal structure of the locked complex showed that C(6) moves 14 A from its normal position to bind in a cytosine-specific pocket on the surface of Tus. ..
  3. Larkin P, Miller J, Allen R, Chitty J, Gerlach W, Frick S, et al. Increasing morphinan alkaloid production by over-expressing codeinone reductase in transgenic Papaver somniferum. Plant Biotechnol J. 2007;5:26-37 pubmed
    ..No significant changes in alkaloid profiles or quantities were observed in leaf, roots, pollen and seed...
  4. Sutcliffe E, Parish I, He Y, Juelich T, Tierney M, Rangasamy D, et al. Dynamic histone variant exchange accompanies gene induction in T cells. Mol Cell Biol. 2009;29:1972-86 pubmed publisher
    ..From these observations, we propose that H3.3 deposition may both facilitate chromatin accessibility increases by destabilizing nucleosomes and compete with recruited histone modifiers to alter PTM patterns upon gene induction. ..
  5. Gorgani N, Parish C, Easterbrook Smith S, Altin J. Histidine-rich glycoprotein binds to human IgG and C1q and inhibits the formation of insoluble immune complexes. Biochemistry. 1997;36:6653-62 pubmed
    ..The results show that human HRG binds to C1q and to IgG in a Zn2+-modulated fashion, and that HRG can regulate the formation of IIC in vitro, thus indicating a new functional role for HRG in vivo. ..
  6. Hillier W, Lukins P, Seibert M, Wydrzynski T. Photochemical reactions of photosystem II in ethylene glycol. Biochemistry. 1997;36:76-85 pubmed
    ..As the EG concentration is increased to 80-100%, there is an irreversible loss of the primary charge separation. The use of EG as a cryoprotectant and as a water-miscible organic solvent for PSII is discussed. ..
  7. Vogalis F, Shiraki T, Kojima D, Wada Y, Nishiwaki Y, Jarvinen J, et al. Ectopic expression of cone-specific G-protein-coupled receptor kinase GRK7 in zebrafish rods leads to lower photosensitivity and altered responses. J Physiol. 2011;589:2321-48 pubmed publisher
    ..These results help provide an understanding for the molecular basis of the electrophysiological differences between cones and rods. ..
  8. Summers R, Dave A, Dolstra T, Bellanca S, Marchetti R, Nash M, et al. Diverse mutational pathways converge on saturable chloroquine transport via the malaria parasite's chloroquine resistance transporter. Proc Natl Acad Sci U S A. 2014;111:E1759-67 pubmed publisher
    ..The finding that diverse PfCRT variants are all limited in their capacity to transport CQ suggests that resistance could be overcome by reoptimizing the CQ dosage. ..
  9. Barratt B, Easton C, Henry D, Li I, Radom L, Simpson J. Inhibition of peptidylglycine alpha-amidating monooxygenase by exploitation of factors affecting the stability and ease of formation of glycyl radicals. J Am Chem Soc. 2004;126:13306-11 pubmed
    ..7 kJ mol(-1), decreases the rate of bromination with N-bromosuccinimide at reflux in carbon tetrachloride by a factor of at least 2000, and stops catalysis by the monooxygenase, while maintaining binding to the enzyme. ..
  10. Förster B, Osmond C, Pogson B. De novo synthesis and degradation of Lx and V cycle pigments during shade and sun acclimation in avocado leaves. Plant Physiol. 2009;149:1179-95 pubmed publisher
  11. Watson M, Liu J, Ollis D. Directed evolution of trimethoprim resistance in Escherichia coli. FEBS J. 2007;274:2661-71 pubmed
    ..Several mutations were found to grant resistance to trimethoprim, both by reducing the binding affinity of the enzyme for the drug, and by increasing the activity of the enzyme. ..
  12. Fairweather S, Bröer A, O Mara M, Broer S. Intestinal peptidases form functional complexes with the neutral amino acid transporter B(0)AT1. Biochem J. 2012;446:135-48 pubmed publisher
  13. Williams N, Liepinsh E, Watt S, Prosselkov P, Matthews J, Attard P, et al. Stabilization of native protein fold by intein-mediated covalent cyclization. J Mol Biol. 2005;346:1095-108 pubmed
    ..Global unfolding of the N-terminal domain of E.coli DnaB is thus promoted strongly by spatial separation of the N and C termini, whereas their proximity is much less important for folding. ..
  14. Li Y, Liu J, Lu M, Ma Z, Cai C, Wang Y, et al. Bacterial Expression and Kinetic Analysis of Carboxylesterase 001D from Helicoverpa armigera. Int J Mol Sci. 2016;17:493 pubmed publisher
    ..armigera. ..
  15. Ozawa K, Headlam M, Schaeffer P, Henderson B, Dixon N, Otting G. Optimization of an Escherichia coli system for cell-free synthesis of selectively N-labelled proteins for rapid analysis by NMR spectroscopy. Eur J Biochem. 2004;271:4084-93 pubmed
    ..In addition, the activity of peptide deformylase appeared to be generally reduced in the cell-free expression system. ..
  16. Stamford N, Lilley P, Dixon N. Enriched sources of Escherichia coli replication proteins. The dnaG primase is a zinc metalloprotein. Biochim Biophys Acta. 1992;1132:17-25 pubmed
    ..Potential zinc-binding amino-acid residues near the N-terminus of the protein were identified. Although a mutant primase lacking 27 amino acid residues from the N-terminus was partly soluble, it was completely inactive. ..
  17. Jones A, Hulett M, Altin J, Hogg P, Parish C. Plasminogen is tethered with high affinity to the cell surface by the plasma protein, histidine-rich glycoprotein. J Biol Chem. 2004;279:38267-76 pubmed
  18. Burnell J, Hatch M. Photosynthesis in phosphoenolpyruvate carboxykinase-type C4 plants: pathways of C4 acid decarboxylation in bundle sheath cells of Urochloa panicoides. Arch Biochem Biophys. 1988;260:187-99 pubmed
    ..We concluded that both PEP carboxykinase and mitochondrial NAD-malic enzyme contribute to C4 acid decarboxylation in these cells, with the required ATP being derived from oxidation-linked phosphorylation in mitochondria. ..
  19. McKenzie H, Frier R. Behavior of S1- and S2-ovalbumin and S-ovalbumin A1 in urea solution: kinetics and equilibria. J Protein Chem. 2003;22:215-20 pubmed
    ..The unfolding of S1- and S2-ovalbumin is an order of magnitude slower than that of R-ovalbumin. Some possible structural differences between R- and S-ovalbumin forms and their significance are discussed. ..
  20. McKenzie H, Frier R. The behavior of R-ovalbumin and its individual components A1, A2, and A3 in urea solution: kinetics and equilibria. J Protein Chem. 2003;22:207-14 pubmed
    ..Unfolding equilibrium profiles were also determined by ORD at several wavelengths and were not stigmoidal in shape and the normalized curves were not superimposed. ..
  21. Hurne A, Chai C, Moerman K, Waring P. Influx of calcium through a redox-sensitive plasma membrane channel in thymocytes causes early necrotic cell death induced by the epipolythiodioxopiperazine toxins. J Biol Chem. 2002;277:31631-8 pubmed
    ..Necrotic but not apoptotic cell death was abrogated by inhibition of calcium entry. ..
  22. Watts R, Hunt P, Hvitved A, Hargrove M, Peacock W, Dennis E. A hemoglobin from plants homologous to truncated hemoglobins of microorganisms. Proc Natl Acad Sci U S A. 2001;98:10119-24 pubmed publisher
    ..Our analysis of the sequence, ligand interactions, and expression profile of GLB3 indicates that this protein has unique biochemical properties, evolutionary history, and, most likely, a function distinct from those of other plant Hbs...
  23. Noor S, Taylor M, Russell R, Jermiin L, Jackson C, Oakeshott J, et al. Intramolecular epistasis and the evolution of a new enzymatic function. PLoS ONE. 2012;7:e39822 pubmed publisher
    ..The mechanistic basis for the epistatic relationships is consistent with a model for the catalytic mechanisms in which protonation is required for hydrolysis of melamine, but not atrazine. ..
  24. Rao S, Gerondakis S, Woltring D, Shannon M. c-Rel is required for chromatin remodeling across the IL-2 gene promoter. J Immunol. 2003;170:3724-31 pubmed
    ..These results suggest a nonredundant role for c-Rel in generating a correctly remodeled chromatin state across the IL-2 promoter and imply that the strength of the signal determines the requirement for c-Rel. ..
  25. Flanigan I, Collins J, Arora K, MacLeod J, Williams J. Exchange reactions catalyzed by group-transferring enzymes oppose the quantitation and the unravelling of the identify of the pentose pathway. Eur J Biochem. 1993;213:477-85 pubmed
    ..1-6.4 rather than the mandatory value of 2 which is imposed by the theoretical mechanism of the pathway. ..
  26. Board P, Coggan M, Chelvanayagam G, Easteal S, Jermiin L, Schulte G, et al. Identification, characterization, and crystal structure of the Omega class glutathione transferases. J Biol Chem. 2000;275:24798-806 pubmed
    ..Unlike other mammalian GSTs, GSTO 1-1 appears to have an active site cysteine that can form a disulfide bond with glutathione. ..
  27. Young Park A, Elvin C, Hamdan S, Wood R, Liyou N, Hamwood T, et al. Hydrolysis of the 5'-p-nitrophenyl ester of TMP by oligoribonucleases (ORN) from Escherichia coli, Mycobacterium smegmatis, and human. Protein Expr Purif. 2008;57:180-7 pubmed
    ..2-0.6 mM. The availability of large amounts of the purified enzymes and a simple spectrophotometric assay for ORN activity should facilitate large-scale screening for new inhibitors of bacterial oligoribonucleases. ..
  28. Tetlow N, Coggan M, Casarotto M, Board P. Functional polymorphism of human glutathione transferase A3: effects on xenobiotic metabolism and steroid biosynthesis. Pharmacogenetics. 2004;14:657-63 pubmed
    ..However, the L71 isoform displayed diminished stability at 45 degrees C. If this relative instability is mirrored in vivo, testosterone and progesterone synthesis may be affected in individuals carrying this allele. ..
  29. Stevenson B, Liu J, Ollis D. Directed evolution of yeast pyruvate decarboxylase 1 for attenuated regulation and increased stability. Biochemistry. 2008;47:3013-25 pubmed publisher
    ..This allowed rapid preparation of libraries for analysis of activity in crude lysates and can be applied to other directed evolution projects. ..
  30. Vasudevan S, Shaw D, Armarego W. Dihydropteridine reductase from Escherichia coli. Biochem J. 1988;255:581-8 pubmed
    ..The pure reductase has a yellow colour and contains bound FAD. The enzyme also has pterin-independent NADH and NADPH oxidoreductase activities when potassium ferricyanide is the electron acceptor. ..
  31. Campbell P, Oakeshott J, Healy M. Purification and kinetic characterisation of juvenile hormone esterase from Drosophila melanogaster. Insect Biochem Mol Biol. 1998;28:501-15 pubmed
    ..Co-incubation of the haemolymph JH binding protein, lipophorin, with JHE indicates lipophorin might modulate JH hydrolysis by competition for binding of JH. ..
  32. Crone E, Sutherland T, Campbell P, Coppin C, Russell R, Oakeshott J. Only one esterase of Drosophila melanogaster is likely to degrade juvenile hormone in vivo. Insect Biochem Mol Biol. 2007;37:540-9 pubmed
    ..Genomic comparisons of the selective JHE across 11 other Drosophila species showed a single orthologue in 10 of them but Drosophila willistoni has 16 full-length copies, five of them with the GQSAG motif and amphipathic helix. ..
  33. John M, Pintacuda G, Park A, Dixon N, Otting G. Structure determination of protein-ligand complexes by transferred paramagnetic shifts. J Am Chem Soc. 2006;128:12910-6 pubmed
    ..The binding mode of thymidine was found to be very similar to that of thymidine monophosphate present in the crystal structure...
  34. Bröer A, Tietze N, Kowalczuk S, Chubb S, Munzinger M, Bak L, et al. The orphan transporter v7-3 (slc6a15) is a Na+-dependent neutral amino acid transporter (B0AT2). Biochem J. 2006;393:421-30 pubmed
    ..A mouse B(0)AT2-like transport activity was detected in synaptosomes and cultured neurons. A potential role of B(0)AT2 in transporting neurotransmitter precursors and neuromodulators is proposed. ..
  35. Murphy J, Ford S, Olsen J, Gustin S, Jeffrey P, Ollis D, et al. Interleukin-3 binding to the murine betaIL-3 and human betac receptors involves functional epitopes formed by domains 1 and 4 of different protein chains. J Biol Chem. 2004;279:26500-8 pubmed
  36. Hamdan S, Bulloch E, Thompson P, Beck J, Yang J, Crowther J, et al. Hydrolysis of the 5'-p-nitrophenyl ester of TMP by the proofreading exonuclease (epsilon) subunit of Escherichia coli DNA polymerase III. Biochemistry. 2002;41:5266-75 pubmed
  37. Mirza S, Walker A, Chen J, Murphy J, Young I. The Ig-like domain of human GM-CSF receptor alpha plays a critical role in cytokine binding and receptor activation. Biochem J. 2010;426:307-17 pubmed publisher
    ..The results indicate a previously unrecognized role for the Ig-like domain, and in particular Cys60, of GM-CSFRalpha in the binding of GM-CSF and subsequent activation of cellular signalling. ..
  38. Gentet L, Clements J. Binding site stoichiometry and the effects of phosphorylation on human alpha1 homomeric glycine receptors. J Physiol. 2002;544:97-106 pubmed
    ..The model suggests that only three binding sites on the glycine channel are functional, while the remaining two binding sites are 'silent', possibly due to strong negative cooperativity. ..
  39. Hayward D, Delaney S, Campbell H, Ghysen A, Benzer S, Kasprzak A, et al. The sluggish-A gene of Drosophila melanogaster is expressed in the nervous system and encodes proline oxidase, a mitochondrial enzyme involved in glutamate biosynthesis. Proc Natl Acad Sci U S A. 1993;90:2979-83 pubmed
    ..Significantly, it has previously been reported that a mouse mutant, PRO/Re, which has reduced proline oxidase activity and elevated free proline levels, also exhibits sluggish behavior. ..
  40. Busch F. Current methods for estimating the rate of photorespiration in leaves. Plant Biol (Stuttg). 2013;15:648-55 pubmed publisher
    ..Six different methods used to estimate photorespiration are reviewed, and their advantages and disadvantages discussed. ..
  41. Dowd C, Wilson I, McFadden H. Gene expression profile changes in cotton root and hypocotyl tissues in response to infection with Fusarium oxysporum f. sp. vasinfectum. Mol Plant Microbe Interact. 2004;17:654-67 pubmed publisher
    ..Gene expression results implicated the phytohormones ethylene and auxin in the disease process. Biochemical analysis of hormone level changes supported this observation...
  42. Pedroso M, Ely F, Carpenter M, Mitic N, Gahan L, Ollis D, et al. Mechanistic Insight from Calorimetric Measurements of the Assembly of the Binuclear Metal Active Site of Glycerophosphodiesterase (GpdQ) from Enterobacter aerogenes. Biochemistry. 2017;56:3328-3336 pubmed publisher
  43. Hopkins D, Fraser N, Mabbitt P, Carr P, Oakeshott J, Jackson C. Structure of an Insecticide Sequestering Carboxylesterase from the Disease Vector Culex quinquefasciatus: What Makes an Enzyme a Good Insecticide Sponge?. Biochemistry. 2017;56:5512-5525 pubmed publisher
    ..This provides a molecular understanding of pesticide sequestration by insect CBEs and could facilitate the design of CBE-specific inhibitors to circumvent this resistance mechanism in the future...