HPLC time-of-flight mass spectrometer for accurate mass measurements

Summary

Principal Investigator: Richard van Breemen
Abstract: [unreadable] DESCRIPTION (provided by applicant): A high performance liquid chromatograph time-of-flight (LC-TOF) mass spectrometer will be purchased and used for accurate mass measurements in support of the research of a multidisciplinary group of NIH-funded users (including two multi-investigator center projects). In addition, minor users and new users at the University of Illinois at Chicago as well as nearby Northwestern University and the University of Chicago will be provided access to the proposed LC-TOF mass spectrometer. The LC-TOF mass spectrometer will complement the capabilities of an existing JEOL GCMate mass spectrometer by providing accurate mass measurements using electrospray and/or atmospheric pressure chemical ionization (APCI). Electrospray, APCI, and HPLC are not available on the GCMate. In addition, the new LC-TOF system will provide combined accurate mass measurement with on- line HPLC separation, which is not currently available to this user group, as well as higher throughput than our existing instrumentation. The LC-TOF mass spectrometer will be located within a campus-wide shared instrumentation laboratory called the Research Resources Center (RRC). The RRC is a centralized campus instrumentation facility that has been in operation for over 50 years and is under the oversight of the Vice Chancellor for Research. Access to the instrument by new and continuing users on and off campus will be assured through the administrative oversight of an advisory committee appointed by the RRC. In anticipation of the expansion of our accurate mass measurement service, the RRC recently hired a Ph.D. level mass spectrometrist to oversee the operation of the new LC-TOF mass spectrometer. This new staff position is committed for at least the useful lifetime of the proposed LC-TOF mass spectrometer. In support of this and other Ph.D.-level mass spectrometrists at the RRC, the P.I. (who has more than 20 years of experience managing mass spectrometry facilities) provides oversight to the RRC and advice to the users of the RRC Mass Spectrometry Laboratory. These on-going personnel commitments represent substantial support by the university. User fees (determined by the advisory committee) will be collected to defray the costs of consumable supplies and repairs. Overall, the proposed LC-TOF mass spectrometer will expand our capacity to provide accurate mass measurements to natural product chemists and synthetic organic chemists in the user community who rely upon rapid analyses of their compounds in order to advance with their NIH-funded research programs [unreadable] [unreadable] [unreadable]
Funding Period: 2007-05-01 - 2009-04-30
more information: NIH RePORT

Top Publications

  1. pmc Screening antioxidants using LC-MS: case study with cocoa
    Angela I Calderón
    Department of Medicinal Chemistry and Pharmacognosy, University of Illinois College of Pharmacy, Chicago, Illinois 60612, USA
    J Agric Food Chem 57:5693-9. 2009
  2. pmc Discovery of cyclooxygenase inhibitors from medicinal plants used to treat inflammation
    Hongmei Cao
    Department of Medicinal Chemistry and Pharmacognosy, University of Illinois College of Pharmacy, 833 S Wood Street, Chicago, IL, USA
    Pharmacol Res 61:519-24. 2010
  3. pmc Screening natural products for inhibitors of quinone reductase-2 using ultrafiltration LC-MS
    Yongsoo Choi
    Department of Medicinal Chemistry and Pharmacognosy, University of Illinois College of Pharmacy, Chicago, Illinois 60612, USA
    Anal Chem 83:1048-52. 2011

Detail Information

Publications4

  1. pmc Screening antioxidants using LC-MS: case study with cocoa
    Angela I Calderón
    Department of Medicinal Chemistry and Pharmacognosy, University of Illinois College of Pharmacy, Chicago, Illinois 60612, USA
    J Agric Food Chem 57:5693-9. 2009
    ..This new LC-MS assay facilitates the rapid identification and then the determination of the relative antioxidant activities of individual antioxidant species in complex natural product samples and food products such as cocoa...
  2. pmc Discovery of cyclooxygenase inhibitors from medicinal plants used to treat inflammation
    Hongmei Cao
    Department of Medicinal Chemistry and Pharmacognosy, University of Illinois College of Pharmacy, 833 S Wood Street, Chicago, IL, USA
    Pharmacol Res 61:519-24. 2010
    ..Roburic acid and phenethyl-trans-ferulate inhibited COX-1 and COX-2 equally. COX inhibition and the IC(50) values of most of these natural product ligands have not been reported previously...
  3. pmc Screening natural products for inhibitors of quinone reductase-2 using ultrafiltration LC-MS
    Yongsoo Choi
    Department of Medicinal Chemistry and Pharmacognosy, University of Illinois College of Pharmacy, Chicago, Illinois 60612, USA
    Anal Chem 83:1048-52. 2011
    ..Ultrafiltration LC-MS was shown to be a useful assay for the discovery of inhibitors of QR-2 in complex matrixes such as extracts of bacteria and botanicals...