Vision Research Infrastructure Development Grant

Summary

Principal Investigator: JOE GILBERT HOLLYFIELD
Abstract: [unreadable] DESCRIPTION (provided by applicant): The vision research program of the Cleveland Clinic Foundation's (CCF) Cole Eye Institute proposes a program of research infrastructure development that will facilitate the study of the structure, development, and function of the eye and visual system in health and disease, with the goal of preventing or curing disorders of the eye that lead to blindness. The investigators benefiting from this support use the most sophisticated research techniques available, including those of genomics, proteomics, electrophysiology, and cell and molecular biology. The two resource modules that are proposed will help achieve this goal: [unreadable] [unreadable] I. Proteomics/Protein Chemistry Resource Module (Assistance in protein identification using amino acid analysis, 2D gel electrophoresis, mass spectroscopy for custom research needs.) [unreadable] [unreadable] II. Microscopy/Histology Resource Module (Histology support for tissue analysis using confocal microscopy, electron microscopy, brightfield, darkfield, phase contrast or fluorescence microscopy, immunocytochemistry, and computer-aided image analysis.) [unreadable] [unreadable] This is a new application submitted by the Principal Investigator and 15 other funded vision scientists. The scientists include six with eight eligible NEI grants. The nine other basic and clinical investigators are funded by other sources. Of the latter, four have submitted NEI R01 applications, and two others plan to do so in the future. The CCF has committed to support the Vision Research Core Center by constructing new laboratory space; purchasing a wide range of new equipment for the resource modules; providing salary support for an administrator to manage the grant; and providing partial support for investigator salaries. This Vision Research Infrastructure Development Grant will bring important Core Resource services needed by these investigators. Their research activities will be enhanced by these services and their collaborative interactions will be strengthened and extended through this program. [unreadable] [unreadable]
Funding Period: 2004-06-01 - 2010-05-31
more information: NIH RePORT

Top Publications

  1. pmc Assessing susceptibility to age-related macular degeneration with proteomic and genomic biomarkers
    Jiayin Gu
    Cole Eye Institute, Lerner Research Inst, Cleveland Clinic Foundation, 9500 Euclid Ave, Cleveland, OH 44195, USA
    Mol Cell Proteomics 8:1338-49. 2009
  2. pmc Betacellulin induces increased retinal vascular permeability in mice
    Bela Anand-Apte
    Department of Ophthalmology, Cole Eye Institute, Cleveland Clinic Lerner College of Medicine, Cleveland, Ohio, United States of America
    PLoS ONE 5:e13444. 2010
  3. pmc Impaired function of circulating CD34(+) CD45(-) cells in patients with proliferative diabetic retinopathy
    Kevin Tan
    Department of Ophthalmology, Cleveland Clinic Lerner College of Medicine, Cleveland Clinic, Cleveland, OH 44195, USA
    Exp Eye Res 91:229-37. 2010
  4. pmc Cross-talk between vascular endothelial growth factor and matrix metalloproteinases in the induction of neovascularization in vivo
    Quteba Ebrahem
    Department of Ophthalmology, Cole Eye Institute, Cleveland Clinic Lerner College of Medicine, Cleveland, OH 44195, USA
    Am J Pathol 176:496-503. 2010
  5. ncbi Mutation screen of beta-crystallin genes in 274 patients with age-related macular degeneration
    Gwen M Sturgill
    Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, Ohio, USA
    Ophthalmic Genet 31:129-34. 2010
  6. pmc Differences in the early biomechanical effects of hyperopic and myopic laser in situ keratomileusis
    Fabricio Witzel de Medeiros
    Cole Eye Institute, Cleveland Clinic, Cleveland, Ohio 44195, USA
    J Cataract Refract Surg 36:947-53. 2010
  7. pmc Preliminary quantitative proteomic characterization of glaucomatous rat retinal ganglion cells
    John W Crabb
    Cole Eye Institute i31, Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA
    Exp Eye Res 91:107-10. 2010
  8. pmc Light-evoked responses of the retinal pigment epithelium: changes accompanying photoreceptor loss in the mouse
    IVY S SAMUELS
    Research Service, Louis Stokes Cleveland VA Medical Center, Cleveland, Ohio 44106, USA
    J Neurophysiol 104:391-402. 2010
  9. pmc A novel transgenic zebrafish model for blood-brain and blood-retinal barrier development
    Jing Xie
    Department of Ophthalmic Research, Cole Eye Institute, Cleveland Clinic, Cleveland, OH, USA
    BMC Dev Biol 10:76. 2010
  10. pmc A hapten generated from an oxidation fragment of docosahexaenoic acid is sufficient to initiate age-related macular degeneration
    Joe G Hollyfield
    Cole Eye Institute, Cleveland Clinic Lerner College of Medicine, Cleveland, OH, USA
    Mol Neurobiol 41:290-8. 2010

Detail Information

Publications109 found, 100 shown here

  1. pmc Assessing susceptibility to age-related macular degeneration with proteomic and genomic biomarkers
    Jiayin Gu
    Cole Eye Institute, Lerner Research Inst, Cleveland Clinic Foundation, 9500 Euclid Ave, Cleveland, OH 44195, USA
    Mol Cell Proteomics 8:1338-49. 2009
    ..We conclude that CEP plasma biomarkers, particularly in combination with genomic markers, offer a potential early warning system for assessing susceptibility to this blinding, multifactorial disease...
  2. pmc Betacellulin induces increased retinal vascular permeability in mice
    Bela Anand-Apte
    Department of Ophthalmology, Cole Eye Institute, Cleveland Clinic Lerner College of Medicine, Cleveland, Ohio, United States of America
    PLoS ONE 5:e13444. 2010
    ..This led us to hypothesize a role for betacellulin in the retinal vascular complications associated with diabetes...
  3. pmc Impaired function of circulating CD34(+) CD45(-) cells in patients with proliferative diabetic retinopathy
    Kevin Tan
    Department of Ophthalmology, Cleveland Clinic Lerner College of Medicine, Cleveland Clinic, Cleveland, OH 44195, USA
    Exp Eye Res 91:229-37. 2010
    ..This suggests that ECFCs may be a potential therapeutic target in the prevention and treatment of diabetic vascular complications...
  4. pmc Cross-talk between vascular endothelial growth factor and matrix metalloproteinases in the induction of neovascularization in vivo
    Quteba Ebrahem
    Department of Ophthalmology, Cole Eye Institute, Cleveland Clinic Lerner College of Medicine, Cleveland, OH 44195, USA
    Am J Pathol 176:496-503. 2010
    ..Our results suggest that MMPs act both upstream and downstream of VEGF and imply that potential combination therapies of VEGF and MMP inhibitors may be a useful therapeutic approach in diseases of pathological neovascularization...
  5. ncbi Mutation screen of beta-crystallin genes in 274 patients with age-related macular degeneration
    Gwen M Sturgill
    Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, Ohio, USA
    Ophthalmic Genet 31:129-34. 2010
    ..We therefore investigated CRYBB1 and CRYBB2 as candidate genes for AMD in 274 unrelated patients...
  6. pmc Differences in the early biomechanical effects of hyperopic and myopic laser in situ keratomileusis
    Fabricio Witzel de Medeiros
    Cole Eye Institute, Cleveland Clinic, Cleveland, Ohio 44195, USA
    J Cataract Refract Surg 36:947-53. 2010
    ....
  7. pmc Preliminary quantitative proteomic characterization of glaucomatous rat retinal ganglion cells
    John W Crabb
    Cole Eye Institute i31, Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA
    Exp Eye Res 91:107-10. 2010
    ..The results demonstrate the feasibility of identifying global proteomic differences in protein expression between purified glaucomatous and control in vivo RGCs...
  8. pmc Light-evoked responses of the retinal pigment epithelium: changes accompanying photoreceptor loss in the mouse
    IVY S SAMUELS
    Research Service, Louis Stokes Cleveland VA Medical Center, Cleveland, Ohio 44106, USA
    J Neurophysiol 104:391-402. 2010
    ....
  9. pmc A novel transgenic zebrafish model for blood-brain and blood-retinal barrier development
    Jing Xie
    Department of Ophthalmic Research, Cole Eye Institute, Cleveland Clinic, Cleveland, OH, USA
    BMC Dev Biol 10:76. 2010
    ....
  10. pmc A hapten generated from an oxidation fragment of docosahexaenoic acid is sufficient to initiate age-related macular degeneration
    Joe G Hollyfield
    Cole Eye Institute, Cleveland Clinic Lerner College of Medicine, Cleveland, OH, USA
    Mol Neurobiol 41:290-8. 2010
    ..Such a model will allow for the efficient preclinical testing of the much-needed therapeutics quickly and inexpensively...
  11. pmc Stromal interleukin-1 expression in the cornea after haze-associated injury
    F L Barbosa
    Cole Eye Institute, The Cleveland Clinic, 9500 Euclid Ave, Cleveland, OH 44195, USA
    Exp Eye Res 91:456-61. 2010
    ..However, some SMA+ myofibroblasts themselves produce IL-1alpha and/or IL-1beta, suggesting that myofibroblast viability could also be regulated via autocrine mechanisms...
  12. pmc Photoreceptor degeneration, azoospermia, leukoencephalopathy, and abnormal RPE cell function in mice expressing an early stop mutation in CLCN2
    Malia M Edwards
    Jackson Laboratory, Bar Harbor, Maine, USA
    Invest Ophthalmol Vis Sci 51:3264-72. 2010
    ..To determine the molecular basis and the pathologic consequences of a chemically induced mutation in a mouse model of photoreceptor degeneration, nmf240...
  13. pmc Corneal myofibroblast generation from bone marrow-derived cells
    Flavia L Barbosa
    Cole Eye Institute, The Cleveland Clinic, 9500 Euclid Ave, Cleveland, OH 44195, USA
    Exp Eye Res 91:92-6. 2010
    ..This model, therefore, cannot exclude the possibility of myofibroblasts also developed from keratocytes and/or corneal fibroblasts...
  14. pmc Femtosecond laser in laser in situ keratomileusis
    Marcella Q Salomao
    Cole Eye Institute, Cleveland Clinic, Cleveland, Ohio 44195, USA
    J Cataract Refract Surg 36:1024-32. 2010
    ..In this article, we review common considerations in management and complications of this step in femtosecond laser-LASIK and concentrate primarily on the IntraLase laser because most published studies relate to this instrument...
  15. ncbi Proteomic and genomic biomarkers for age-related macular degeneration
    Jiayin Gu
    Cole Eye Institute, Cleveland, OH, USA
    Adv Exp Med Biol 664:411-7. 2010
    ..CEP plasma biomarkers, particularly in combination with genomic markers, offer a potential early warning system for predicting susceptibility to this blinding disease...
  16. pmc Retinal pathology of a patient with Goldmann-Favre syndrome
    Vera L Bonilha
    The Cole Eye Institute, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Ophthalmic Genet 30:172-80. 2009
    ..To define the retinal pathology in an 88-year-old male affected with Goldmann-Favre syndrome with a 2 bp 5' A>C splice site mutation in the NR2E3 gene...
  17. pmc Effect of femtosecond laser energy level on corneal stromal cell death and inflammation
    Fabricio Witzel de Medeiros
    Cleveland Clinic, Cole Eye Institute, Ohio 44195, USA
    J Refract Surg 25:869-74. 2009
    ..To analyze the effects of variations in femtosecond laser energy level on corneal stromal cell death and inflammatory cell influx following flap creation in a rabbit model...
  18. pmc Dynamics of the expression of intermediate filaments vimentin and desmin during myofibroblast differentiation after corneal injury
    Shyam S Chaurasia
    Cole Eye Institute, Cleveland Clinic, Cleveland, OH 44195, USA
    Exp Eye Res 89:133-9. 2009
    ..The earliest precursors of myofibroblasts destined to express SMA and desmin are detectible by staining for vimentin at 1 week after surgery...
  19. pmc Early synaptic defects in tulp1-/- mice
    Gregory H Grossman
    Department of Ophthalmic Research, Cole Eye Institute, Cleveland Clinic, Cleveland, Ohio, USA
    Invest Ophthalmol Vis Sci 50:3074-83. 2009
    ..To investigate the role of Tulp1 in the photoreceptor synapse, the authors examined the presynaptic and postsynaptic architecture and retinal function in tulp1(-/-) mice..
  20. pmc Expression of PDGF receptor-alpha in corneal myofibroblasts in situ
    Harmeet Kaur
    Cole Eye Institute, The Cleveland Clinic, 9500 Euclid Ave, Cleveland, OH 44195, USA
    Exp Eye Res 89:432-4. 2009
    ..In vitro studies have suggested that PDGF is important in the development and viability of myofibroblasts after corneal injury. Expression of PDGFR-alpha in myofibroblasts supports these findings...
  21. pmc Corneal myofibroblast viability: opposing effects of IL-1 and TGF beta1
    Harmeet Kaur
    Cole Eye Institute, The Cleveland Clinic, Cleveland, OH 44195, USA
    Exp Eye Res 89:152-8. 2009
    ..TGF beta1 and IL-1 have opposing effects on myofibroblast viability and likely interact to modulate haze generation after corneal injury...
  22. pmc Plasma protein pentosidine and carboxymethyllysine, biomarkers for age-related macular degeneration
    Jiaqian Ni
    Cole Eye Institute, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Mol Cell Proteomics 8:1921-33. 2009
    ....
  23. pmc Genetic analysis of complement factor H related 5, CFHR5, in patients with age-related macular degeneration
    Umadevi Narendra
    Department of Ophthalmic Research, Cole Eye Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    Mol Vis 15:731-6. 2009
    ..To investigate the complement factor H related 5 (CFHR5) gene, encoding a member of the complement factor H family, for the presence of genetic polymorphisms or mutations associated with age-related macular degeneration (AMD)...
  24. pmc Protective effect of paraoxonase 1 gene variant Gln192Arg in age-related macular degeneration
    Gayle J T Pauer
    Department of Ophthalmic Research, Cole Eye Institute, Cleveland Clinic, Cleveland, Ohio 44195, USA
    Am J Ophthalmol 149:513-22. 2010
    ..Paraoxonase 1 (PON1) protects against oxidative damage and has been evaluated for its involvement in aging diseases including AMD. This study investigated whether PON1 gene polymorphisms associate with AMD...
  25. pmc Quantitative proteomics: comparison of the macular Bruch membrane/choroid complex from age-related macular degeneration and normal eyes
    Xianglin Yuan
    Cole Eye Institute, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Mol Cell Proteomics 9:1031-46. 2010
    ....
  26. pmc Biomechanical corneal changes induced by different flap thickness created by femtosecond laser
    Fabricio W Medeiros
    Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, Brazil
    Clinics (Sao Paulo) 66:1067-71. 2011
    ..To evaluate the impact of the creation of corneal flaps at different thicknesses on the biomechanical properties of swine corneas...
  27. pmc Tissue inhibitor of metalloproteinases-3 peptides inhibit angiogenesis and choroidal neovascularization in mice
    Jian Hua Qi
    Department of Ophthalmology, Cole Eye Institute, Cleveland Clinic Lerner College of Medicine at Case Western Reserve University, Cleveland, Ohio, United States of America
    PLoS ONE 8:e55667. 2013
    ..Thus, we have identified TIMP-3 peptides to be efficient inhibitors of angiogenesis and have a potential to be used therapeutically in diseases with increased neovascularization...
  28. pmc Transmission electron microscopy analysis of epithelial basement membrane repair in rabbit corneas with haze
    André A M Torricelli
    Cole Eye Institute, Cleveland Clinic, Cleveland, OH 44195, USA
    Invest Ophthalmol Vis Sci 54:4026-33. 2013
    ..To assess the ultrastructure of the epithelial basement membrane using transmission electron microscopy (TEM) in rabbit corneas with and without subepithelial stroma opacity (haze)...
  29. pmc Protein partners of dynamin-1 in the retina
    Gregory H Grossman
    Department of Ophthalmic Research, Cleveland Clinic Cole Eye Institute, Cleveland, Ohio
    Vis Neurosci 30:129-39. 2013
    ..This finding is confirmed by western blot analysis. Results further implicate dynamin-1 in vesicular protein transport processes relevant to synaptic and post-Golgi pathways and indicate a possible role in photoreceptor stability...
  30. pmc Mouse strain variation in SMA(+) myofibroblast development after corneal injury
    Vivek Singh
    The Cole Eye Institute, Cleveland Clinic, 9500 Euclid Ave, Cleveland, OH 44195, USA
    Exp Eye Res 115:27-30. 2013
    ..Further investigation of genetics underlying mouse strain variation could provide insight into the corneal wound healing and haze generation processes. ..
  31. pmc Inhibition of EGF signaling protects the diabetic retina from insulin-induced vascular leakage
    Masahiko Sugimoto
    Department of Ophthalmology, Cole Eye Institute, Cleveland Clinic Lerner College of Medicine, Cleveland Clinic, Cleveland, Ohio 44195, USA
    Am J Pathol 183:987-95. 2013
    ..These findings provide unique insight into the role of insulin signaling in mediating retinal effects in diabetes and open new avenues for therapeutics to treat the retinal complications of diabetes mellitus. ..
  32. pmc A method to generate enhanced GFP+ chimeric mice to study the role of bone marrow-derived cells in the eye
    Vivek Singh
    Cole Eye Institute, Cleveland Clinic, 9500 Euclid Ave, Cleveland, OH 44195, USA
    Exp Eye Res 116:366-70. 2013
    ..The GFP+ bone marrow chimeric mouse provides an excellent model for studying the role of bone marrow-derived cells in corneal wound healing, glaucoma surgery, optic nerve head pathology and retinal pathophysiology and wound healing. ..
  33. pmc The corneal epithelial basement membrane: structure, function, and disease
    André A M Torricelli
    Cole Eye Institute, Cleveland Clinic, Cleveland, Ohio
    Invest Ophthalmol Vis Sci 54:6390-400. 2013
    ..The corneal epithelial BM is also involved in many inherited and acquired corneal diseases. This review examines this structure in detail and discusses the importance of corneal epithelial BM in homeostasis, wound healing, and disease...
  34. pmc CEP biomarkers as potential tools for monitoring therapeutics
    Kutralanathan Renganathan
    Cole Eye Institute, Cleveland Clinic Foundation, Cleveland, Ohio, United States of America Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio, United States of America Department of Chemistry, Case Western Reserve University, Cleveland, Ohio, United States of America
    PLoS ONE 8:e76325. 2013
    ..AL-8309A is a serotonin 5-HT1A receptor agonist...
  35. ncbi Interaction of tubby-like protein-1 (Tulp1) and microtubule-associated protein (MAP) 1A and MAP1B in the mouse retina
    Gregory H Grossman
    Department of Ophthalmic Research, Cole Eye Institute, Cleveland Clinic, Cleveland, OH, USA
    Adv Exp Med Biol 801:511-8. 2014
    ..This may be the cause for disorganized OSs in tulp1-/- mice, and indicate that their transport to the OS is Tulp1-dependent. ..
  36. pmc BAC-EDTA transepithelial riboflavin-UVA crosslinking has greater biomechanical stiffening effect than standard epithelium-off in rabbit corneas
    André A M Torricelli
    Cole Eye Institute, Cleveland Clinic, Cleveland, OH, USA University of Sao Paulo, Sao Paulo, Brazil
    Exp Eye Res 125:114-7. 2014
    ..These results demonstrated that the biomechanical stiffening effect produced by BAC-EDTA transepithelial CXL was greater than that produced by standard epithelium-off CXL in a rabbit model. ..
  37. pmc TGFβ and PDGF-B signaling blockade inhibits myofibroblast development from both bone marrow-derived and keratocyte-derived precursor cells in vivo
    Vivek Singh
    The Cole Eye Institute, Cleveland Clinic, 9500 Euclid Ave, Cleveland, OH 44195, USA LV Prasad Eye Institute, HERF, Hyderabad, AP, India
    Exp Eye Res 121:35-40. 2014
    ..TGFβ and PDGF regulate corneal myofibroblast development from bone marrow-derived precursor cells and keratocyte/corneal fibroblast-derived precursor cells. ..
  38. ncbi Cellular effects after laser in situ keratomileusis flap formation with femtosecond lasers: a review
    Marcony R Santhiago
    Cole Eye Institute, The Cleveland Clinic, Cleveland, OH 44195, USA
    Cornea 31:198-205. 2012
    ..To provide an overview of the cellular effects of femtosecond laser in laser in situ keratomileusis flap formation...
  39. pmc Corneal myofibroblast biology and pathobiology: generation, persistence, and transparency
    Steven E Wilson
    The Cole Eye Institute, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA
    Exp Eye Res 99:78-88. 2012
    ..Investigations of myofibroblast biology are likely to lead to safer pharmacological modulators of corneal wound healing and transparency...
  40. pmc Monocyte development inhibitor PRM-151 decreases corneal myofibroblast generation in rabbits
    M R Santhiago
    Exp Eye Res 93:786-9. 2011
    ..This study provides additional confirmation that bone marrow-derived cells contribute to corneal myofibroblast generation...
  41. pmc Increased neovascularization in mice lacking tissue inhibitor of metalloproteinases-3
    Quteba Ebrahem
    Department of Ophthalmology, Cole Eye Institute, Cleveland Clinic Lerner College of Medicine, Cleveland, Ohio 44195, USA
    Invest Ophthalmol Vis Sci 52:6117-23. 2011
    ..Here, the authors examined the in vivo angiogenic phenotype of ocular vessels in mice deficient in TIMP-3...
  42. pmc Effect of TGFβ and PDGF-B blockade on corneal myofibroblast development in mice
    V Singh
    The Cole Eye Institute, The Cleveland Clinic, 9500 Euclid Ave, Cleveland, OH 44195, USA
    Exp Eye Res 93:810-7. 2011
    ..This study provides in situ evidence that TGFβ and PDGF-B have important roles in modulating myofibroblast generation in the mouse cornea after haze-associated injury...
  43. ncbi Visual abnormalities associated with enhanced optic nerve myelination
    Minzhong Yu
    Department of Ophthalmic Research, Cole Eye Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    Brain Res 1374:36-42. 2011
    ..In older mice, where the Akt-DD optic nerve is markedly thicker than control, it is remarkable that optic nerves continue to function...
  44. ncbi Corneal wound healing after ultraviolet-A/riboflavin collagen cross-linking: a rabbit study
    Marcella Q Salomao
    Cole Eye Institute, Cleveland Clinic, Cleveland, Ohio 44195, USA
    J Refract Surg 27:401-7. 2011
    ..To investigate corneal wound healing following ultraviolet-A (UVA)/riboflavin corneal collagen cross-linking (CXL) in rabbit corneas...
  45. pmc Immunocytochemical evidence of Tulp1-dependent outer segment protein transport pathways in photoreceptor cells
    Gregory H Grossman
    Department of Ophthalmic Research, i31, Cole Eye Institute, Cleveland Clinic, 9500 Euclid Avenue, Cleveland, OH 44195, USA
    Exp Eye Res 93:658-68. 2011
    ..However, without Tulp1, two rhodopsin transport machinery proteins exhibit abnormal distribution, Rab8 and Rab11, suggesting a role for Tulp1 in vesicular docking and fusion at the plasma membrane near the connecting cilium...
  46. pmc Proteomic similarities in steroid responsiveness in normal and glaucomatous trabecular meshwork cells
    Kathryn E Bollinger
    Cole Eye Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    Mol Vis 18:2001-11. 2012
    ..Mechanisms of GC-signaling are complex and poorly understood. To better understand GC-signaling in the eye, we tested the hypothesis that common mechanisms of steroid responsiveness exist in TM cells from normal and glaucomatous donors...
  47. ncbi Mitomycin C: biological effects and use in refractive surgery
    Marcony R Santhiago
    The Cole Eye Institute, The Cleveland Clinic, Cleveland, OH 44195, USA
    Cornea 31:311-21. 2012
    ..To provide an overview of the safety and efficacy of mitomycin C (MMC) as adjuvant therapy after refractive surgery procedures...
  48. pmc Interleukin-1 receptor role in the viability of corneal myofibroblasts
    F L Barbosa
    The Cole Eye Institute, The Cleveland Clinic Foundation, 9500 Euclid Ave Cleveland, OH 44195, United States
    Exp Eye Res 96:65-9. 2012
    ..Thus, IL-1 is an important modulator of myofibroblast viability during corneal wound healing...
  49. pmc Stromal fibroblast-bone marrow-derived cell interactions: implications for myofibroblast development in the cornea
    V Singh
    Cole Eye Institute, The Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    Exp Eye Res 98:1-8. 2012
    ..These data suggest that TGFβ is a paracrine modulator that regulates the generation of myofibroblasts from either corneal fibroblasts or bone marrow-derived cell precursors...
  50. pmc Transforming growth factor β and platelet-derived growth factor modulation of myofibroblast development from corneal fibroblasts in vitro
    Vivek Singh
    Cole Eye Institute, Cleveland Clinic, Cleveland, OH 44195, USA
    Exp Eye Res 120:152-60. 2014
    ..This study defines optimal in vitro conditions to monitor the development of MSF into myofibroblasts. The combined effects of TGFβ and PDGF promote the full development of V+A+D+ myofibroblasts from MSF. ..
  51. pmc S156C mutation in tissue inhibitor of metalloproteinases-3 induces increased angiogenesis
    Jian Hua Qi
    Department of Opthalmology, Cole Eye Institute, Cleveland Clinic Lerner College of Medicine at Case Western Reserve University, Cleveland, Ohio 44195, USA
    J Biol Chem 284:19927-36. 2009
    ..Understanding the mechanism(s) by which mutant TIMP-3 can induce abnormal neovascularization provides important insight into the pathophysiology of a number of diseases with increased angiogenesis...
  52. ncbi Characterization of semenogelin proteins in the human retina
    Vera L Bonilha
    Department of Ophthalmic Research, The Cole Eye Institute, Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH, 44195, USA
    Exp Eye Res 83:120-7. 2006
    ..Our data support the expression of semenogelin I and II in the human retina in several different compartments. Further studies towards addressing the function of these proteins in the retina are in progress...
  53. ncbi Genetic, age and light mediated effects on crystallin protein expression in the retina
    Daniel Organisciak
    Petticrew Research Laboratory, School of Medicine, Wright State University, Dayton, OH, USA
    Photochem Photobiol 82:1088-96. 2006
    ..Our data suggests that an increase in crystallin expression occurs during various retinal degenerations and that the increases may be related to the severity, type and onset of retinal degeneration...
  54. ncbi Microvilli defects in retinas of ezrin knockout mice
    Vera L Bonilha
    Department of Ophthalmic Research, The Cole Eye Institute, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA
    Exp Eye Res 82:720-9. 2006
    ..Finally, photoreceptors in the ezrin knockout animals show substantial retardation in development as compared to their wild type littermates...
  55. pmc Carboxyethylpyrrole oxidative protein modifications stimulate neovascularization: Implications for age-related macular degeneration
    Quteba Ebrahem
    Cole Eye Institute and Lerner Research Institute, Cleveland Clinic Foundation, and Department of Chemistry, Case Western Reserve University, Cleveland, OH 44195, USA
    Proc Natl Acad Sci U S A 103:13480-4. 2006
    ..Overall, these results suggest that CEP-induced angiogenesis utilizes VEGF-independent pathways and that anti-CEP therapeutic modalities might be of value in limiting CNV in AMD...
  56. pmc Mutation screen of the cone-specific gene, CLUL1, in 376 patients with age-related macular degeneration
    Gwen M Sturgill
    Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, OH, USA
    Ophthalmic Genet 27:151-5. 2006
    ..Variant alleles of the CLUL1 gene were found; however, none are considered pathogenic. None of the variants identified are predicted to create or destroy splice donor or acceptor sites based on splice-site prediction software...
  57. ncbi Glucose utilization by the retinal pigment epithelium: evidence for rapid uptake and storage in glycogen, followed by glycogen utilization
    Preenie deS Senanayake
    Department of Ophthalmic Research, The Cole Eye Institute, The Lerner Research Institute, Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA
    Exp Eye Res 83:235-46. 2006
    ..These studies are the first to demonstrate glucose, glycogen and maltodextrin metabolism by RPE cells, and their detection and quantitation by FACE...
  58. pmc Partial rescue of retinal function and sterol steady-state in a rat model of Smith-Lemli-Opitz syndrome
    Steven J Fliesler
    Department of Ophthalmology, Saint Louis University School of Medicine, St Louis, Missouri 63104, USA
    Pediatr Res 61:273-8. 2007
    ..These findings demonstrate that cholesterol readily crosses the blood-retina barrier (unlike the blood-brain barrier) and suggest that cholesterol supplementation may be efficacious in treating SLOS-associated retinopathy...
  59. pmc Apoptosis in the initiation, modulation and termination of the corneal wound healing response
    Steven E Wilson
    The Cole Eye Institute, The Cleveland Clinic, 9500 Euclid Avenue, Cleveland, OH 44195, USA
    Exp Eye Res 85:305-11. 2007
    ....
  60. pmc Attenuation of oscillatory potentials in nob2 mice
    Minzhong Yu
    Cole Eye Institute I 31, Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA
    Doc Ophthalmol 115:173-86. 2007
    ..In addition to a loss of dark-adapted (rod-driven) visual sensitivity, electroretinogram (ERG) b-waves and oscillatory potentials (OPs) are decreased in CSNB2 patients...
  61. ncbi Angiotensin II and its receptor subtypes in the human retina
    Preenie deS Senanayake
    Department of Ophthalmology, Cole Eye Institute, Cleveland Clinic, Cleveland, Ohio 44195, USA
    Invest Ophthalmol Vis Sci 48:3301-11. 2007
    ..To quantify and evaluate the distribution of angiotensin II (Ang II) and its receptors in the human retina...
  62. pmc Voltage-dependent calcium channel CaV1.3 subunits regulate the light peak of the electroretinogram
    Jiang Wu
    Cole Eye Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    J Neurophysiol 97:3731-5. 2007
    ..3(-/-) littermates. Our results identify Ca(V)1.3 as the principal pore-forming subunit of VDCCs involved in stimulating the ERG LP...
  63. ncbi Wavefront analysis comparison of LASIK outcomes with the femtosecond laser and mechanical microkeratomes
    Fabricio W Medeiros
    Cole Eye Institute, Cleveland Clinic, 9500 Euclid Ave, Cleveland, OH 44195, USA
    J Refract Surg 23:880-7. 2007
    ..To evaluate differences related to ocular aberrations after customized LASIK for myopia using three different microkeratomes...
  64. pmc The nob2 mouse, a null mutation in Cacna1f: anatomical and functional abnormalities in the outer retina and their consequences on ganglion cell visual responses
    Bo Chang
    The Jackson Laboratory, Bar Harbor, Maine, USA
    Vis Neurosci 23:11-24. 2006
    ..Further, the nob2 mouse represents a model system in which to define the signals that guide synapse formation and/or maintenance in the OPL...
  65. ncbi Expression of bestrophin-1, the product of the VMD2 gene, modulates voltage-dependent Ca2+ channels in retinal pigment epithelial cells
    Rita Rosenthal
    Augenklinik, Charite Universitaetsmedizin Berlin, Campus Benjamin Franklin, Berlin, Germany
    FASEB J 20:178-80. 2006
    ....
  66. ncbi Molecular basis for the redox control of nuclear transport of the structural chromatin protein Hmgb1
    George Hoppe
    Cole Eye Institute, Cleveland Clinic, Cleveland, OH 44195, USA
    Exp Cell Res 312:3526-38. 2006
    ..Our cysteine targeted mutational analysis suggests that Cys23 and 45 induce conformational changes in response to oxidative stress, whereas Cys106 appears to be critical for the nucleocytoplasmic shuttling of Hmgb1...
  67. pmc Mapping a new genetic locus for X linked retinitis pigmentosa to Xq28
    A Melamud
    J Med Genet 43:e27. 2006
    ..06, 2.17, and 2.20, respectively. Haplotype analysis revealed segregation of the disease phenotype with markers at Xq28...
  68. ncbi Visualization and characterization of inflammatory cell recruitment and migration through the corneal stroma in endotoxin-induced keratitis
    Eric C Carlson
    Department of Ophthalmic Research, Cole Eye Institute, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Invest Ophthalmol Vis Sci 47:241-8. 2006
    ....
  69. pmc Stromal haze, myofibroblasts, and surface irregularity after PRK
    Marcelo V Netto
    The Cole Eye Institute, The Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    Exp Eye Res 82:788-97. 2006
    ..Late apoptosis appears to have a role in the disappearance of myofibroblasts and haze over time...
  70. pmc Biomechanics and wound healing in the cornea
    William J Dupps
    The Cole Eye Institute, Cleveland Clinic, 9500 Euclid Avenue, OH 44195, USA
    Exp Eye Res 83:709-20. 2006
    ....
  71. pmc The light peak of the electroretinogram is dependent on voltage-gated calcium channels and antagonized by bestrophin (best-1)
    Lihua Y Marmorstein
    Department of Ophthalmology and Vision Science, University of Arizona, Tucson 85711, USA
    J Gen Physiol 127:577-89. 2006
    ..Furthermore, we suggest that the loss of vision associated with BMD is not caused by the same pathologic process as the diminished LP, but rather is caused by as yet unidentified effects of best-1 on other cellular processes...
  72. pmc Functional abnormalities in the retinal pigment epithelium of CFTR mutant mice
    Jiang Wu
    Cole Eye Institute, Cole Eye Institute I 31, Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA
    Exp Eye Res 83:424-8. 2006
    ....
  73. pmc The retinal pigment epithelium apical microvilli and retinal function
    Vera L Bonilha
    Cole Eye Institute, Cleveland Clinic Foundation, OH 44195, USA
    Adv Exp Med Biol 572:519-24. 2006
  74. ncbi Proteomics implicates peptidyl arginine deiminase 2 and optic nerve citrullination in glaucoma pathogenesis
    Sanjoy K Bhattacharya
    Cole Eye Institute, Cleveland Clinic Foundation, Cleveland, Ohio, USA
    Invest Ophthalmol Vis Sci 47:2508-14. 2006
    ..Peptidyl arginine deiminase 2 (PAD2), an enzyme that converts protein arginine to citrulline, was found only in POAG optic nerve and was probed further for a mechanistic role in glaucoma...
  75. ncbi CRALBP ligand and protein interactions
    Zhiping Wu
    Cole Eye Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    Adv Exp Med Biol 572:477-83. 2006
  76. ncbi Annexins in Bruch's membrane and drusen
    Mary E Rayborn
    Department of Ophthalmic Research, Cole Eye Institute, Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, Ohio 44195, USA
    Adv Exp Med Biol 572:75-8. 2006
  77. pmc Effect of prophylactic and therapeutic mitomycin C on corneal apoptosis, cellular proliferation, haze, and long-term keratocyte density in rabbits
    Marcelo V Netto
    The Cole Eye Institute, The Cleveland Clinic, Cleveland, Ohio 44195, USA
    J Refract Surg 22:562-74. 2006
    ..To determine the mechanism through which topical mitomycin C prevents and treats corneal haze after photorefractive keratectomy (PRK) and to examine the effects of dosage and duration of exposure...
  78. ncbi Branch retinal artery occlusion associated with compound heterozygous genotype for methylenetetrahydrofolate reductase
    Martin Heur
    Cole Eye Institute, Cleveland Clinic, 9500 Euclid Avenue, Cleveland, OH 44195, USA
    Doc Ophthalmol 114:163-8. 2007
    ..The patient was diagnosed with BRAO and maintained on coumadin therapy...
  79. ncbi SOD2 knockdown mouse model of early AMD
    Verline Justilien
    Department of Molecular Genetics, University of Florida, Gainesville, Florida, USA
    Invest Ophthalmol Vis Sci 48:4407-20. 2007
    ..To test the hypothesis that oxidative injury to the retinal pigment epithelium (RPE) may lead to retinal damage similar to that associated with the early stages of age-related macular degeneration (AMD)...
  80. pmc Age-related reduction in retinal deimination levels in the F344BN rat
    Sanjoy K Bhattacharya
    Bascom Palmer Eye Institute, 1638 NW 10th Avenue, 706, University of Miami, Miami, FL 33136, USA
    Aging Cell 7:441-4. 2008
    ..Our findings are important to understand late-onset and progressive diseases such as glaucoma, pseudoexfoliation syndrome, age-related macular degeneration and Oguchi's disease...
  81. pmc Oxidative damage-induced inflammation initiates age-related macular degeneration
    Joe G Hollyfield
    Cole Eye Institute i31, Department of Ophthalmology, Cleveland Clinic Lerner College of Medicine, Cleveland, Ohio 44195, USA
    Nat Med 14:194-8. 2008
    ..This new model provides a platform for dissecting the molecular pathology of oxidative damage in the outer retina and the immune response contributing to AMD...
  82. pmc Retinal pigment epithelium lipofuscin proteomics
    Kwok Peng Ng
    Cole Eye Institute and Lerner Research Institute, Cleveland Clinic Foundation, Case Western Reserve University, Cleveland, Ohio 44195, USA
    Mol Cell Proteomics 7:1397-405. 2008
    ....
  83. pmc A novel method for generating corneal haze in anterior stroma of the mouse eye with the excimer laser
    Rajiv R Mohan
    Cole Eye Institute, The Cleveland Clinic, Cleveland, OH 44195, USA
    Exp Eye Res 86:235-40. 2008
    ..This PTK technique for inducing haze in mouse cornea in vivo provides a useful model for studying wound healing and myofibroblast biology in transgenic mice...
  84. pmc Choroideremia: analysis of the retina from a female symptomatic carrier
    Vera L Bonilha
    The Cleveland Clinic Foundation, The Cole Eye Institute, Cleveland, Ohio 44195, USA
    Ophthalmic Genet 29:99-110. 2008
    ..To define the retinal pathology in a 91 year-old affected matriarch of a three-generation choroideremia family with multiple manifesting carriers...
  85. pmc Pigment epithelium-derived factor binds to hyaluronan. Mapping of a hyaluronan binding site
    S Patricia Becerra
    NEI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 283:33310-20. 2008
    ..The HA-binding activity of PEDF may contribute to deposition in the extracellular matrix and to its reported antitumor/antimetastatic effects...
  86. pmc Topical interleukin-1 receptor antagonist inhibits inflammatory cell infiltration into the cornea
    W Michael Stapleton
    Cole Eye Institute, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA
    Exp Eye Res 86:753-7. 2008
    ..Topical IL-1 receptor antagonist could be an effective adjuvant for clinical treatment of corneal conditions in which unwanted inflammation has a role in the pathophysiology of the disorder...
  87. pmc Dry eye associated with laser in situ keratomileusis: Mechanical microkeratome versus femtosecond laser
    Marcella Q Salomao
    Cole Eye Institute, Cleveland Clinic, Cleveland, Ohio 44195, USA
    J Cataract Refract Surg 35:1756-60. 2009
    ..To compare the incidence of laser in situ keratomileusis (LASIK)-associated dry eye and the need for postoperative cyclosporine A treatment after flap creation with a femtosecond laser and a mechanical microkeratome...
  88. ncbi Ankyrin-B is required for coordinated expression of beta-2-spectrin, the Na/K-ATPase and the Na/Ca exchanger in the inner segment of rod photoreceptors
    Krishnakumar Kizhatil
    Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA
    Exp Eye Res 88:57-64. 2009
    ..These results are consistent with a mechanism where ankyrin-B is required to restrict the Na/K-ATPase and Na/Ca exchanger to the inner segment of rod photoreceptors by tethering these membrane proteins to beta-2-spectrin...
  89. pmc Corneal stroma PDGF blockade and myofibroblast development
    Harmeet Kaur
    Cole Eye Institute, The Cleveland Clinic, Cleveland, OH 44195, USA
    Exp Eye Res 88:960-5. 2009
    ..Stromal PDGF-B blockade during the early postoperative period following PRK decreases stromal alphaSMA+ myofibroblast generation. PDGF is an important modulator of myofibroblast development in the cornea...
  90. doi Carboxyethylpyrrole adducts, age-related macular degeneration and neovascularization
    Kutralanathan Renganathan
    Cole Eye Institute, Department of Chemistry, Case Western Reserve University, Cleveland, Ohio, USA
    Adv Exp Med Biol 613:261-7. 2008
  91. pmc Semenogelins in the human retina: Differences in distribution and content between AMD and normal donor tissues
    Vera L Bonilha
    Department of Ophthalmology, The Cole Eye Institute, Cleveland Clinic Lerner College of Medicine, 9500 Euclid Avenue, Cleveland, OH 44195, USA
    Exp Eye Res 86:150-6. 2008
    ..We find the distribution of SgI in the AMD retinas substantially lower than observed in normal retina. SgI localization to photoreceptors and the RPE suggests a possible function related to the ability of these cells to sequester zinc...
  92. pmc Targets of tyrosine nitration in diabetic rat retina
    Xianquan Zhan
    Cole Eye Institute, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Mol Cell Proteomics 7:864-74. 2008
    ..The present results provide new insights regarding tyrosine nitration and its potential role in the molecular mechanisms of diabetic retinopathy...
  93. ncbi Long-term resolution of chronic dry eye symptoms and signs after topical cyclosporine treatment
    Steven E Wilson
    Cole Eye Institute, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Ophthalmology 114:76-9. 2007
    ..To report the resolution of symptoms and signs of chronic dry eye for at least 1 year after completion of a minimum 6-month course of topical 0.05% cyclosporine in a subgroup of patients from 2 practices...
  94. ncbi Electrophysiological responses of the mouse retina to 12C ions
    Walter G Sannita
    INFN, Roma2, Roma, Italy
    Neurosci Lett 416:231-5. 2007
    ..No retinal immunohistological damage was detected. Mice proved a suitable animal model to study radiation-induced phosphenes in vivo and our findings are consistent with an origin of phosphenes in radiation activating the retina...
  95. ncbi Focus on molecules: ezrin
    Vera L Bonilha
    Department of Ophthalmic Research, Cole Eye Institute i31, Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland OH 44195, USA
    Exp Eye Res 84:613-4. 2007
  96. pmc Femtosecond laser and microkeratome corneal flaps: comparison of stromal wound healing and inflammation
    Marcelo V Netto
    The Cole Eye Institute, Cleveland Clinic, Cleveland, Ohio 44195, USA
    J Refract Surg 23:667-76. 2007
    ..To examine early postoperative wound healing in rabbit corneas that had LASIK flaps formed with three different models (15 KHz, 30 KhZ, and 60 KHz) of a femtosecond laser compared with flaps formed with a microkeratome...