Ecosystem disturbance and multiscale transmission of a zoonotic wildlife pathogen

Summary

Principal Investigator: Philip Craig
Affiliation: University of Salford
Country: UK
Abstract: A key element in disease emergence/re-emergence is ecosystem disruption as a result of anthropogenic effects which may be rapid as in tropical deforestation or more subtle as in agricultural expansion and overgrazing in temperate biomes. There is however difficulty in developing suitable models to study ecology of infectious diseases, wherein spatial determinants that meaningfully characterize wildlife reservoir habitat, can be linked in turn to host ecology and to dynamics of pathogen/parasite transmission. Using a multidisciplinary approach, grounded on 5 years of collaborative data collected in China from studies on human exposure, transmission ecology, landscape characterization and epidemiology for a pathogenic zoonotic helminth (Echinococcus multilocularis), we aim to develop a general spatio-deterministic model that provides a practical synthesis of host-landscape ecology and transmission models. This approach will be applied study the ecology of transmission of Echinococcus between canids and small mammal reservoir hosts in a geographically defined zone, the Tibetan Plateau (comprising, Sichuan, Qinghai, and Tibet Autonomous Region), and compare wildlife and semi-domestic transmission patterns with that of a geographically separated endemic focus in Central Asia (Xinjiang/Kazahkstan region). A central goal will be to quantify the impact of ecosystem disruption, through livestock over-grazing, socio-economic effects on land-use, and agriculture or deforestation, on the transmission ecology of the parasite, especially in relation to maintenance of enzootic meta-stability at local and regional scales, and to determine relative zoonotic potential. The integrated spatio-deterministic model will be applied at regional scale and tested using simulations that will enable disease (echinococcosis) risk forecasting and development of optimal control/intervention strategies for high risk communities. 2 main hypotheses will be addressed. Firstly, at ecosystem level (the Plateau); parasite transmission may behave as a meta-stable focus despite inherent and peripheral instabilities. Secondly, spatial variables in the form of landscape characteristics can be linked to parasite transmission dynamics using an integrated modeling approach that takes into account multi-level heterogeneity at habitat, host and parasite domains.
Funding Period: 2000-09-30 - 2008-12-31
more information: NIH RePORT

Top Publications

  1. ncbi Nothing is perfect! Trouble-shooting in immunological and molecular studies of cestode infections
    Akira Ito
    Department of Parasitology, Asahikawa Medical University, Asahikawa 078 8510, Japan
    Parasitology 140:1551-65. 2013
  2. pmc Redescription of Heligmosomoides neopolygyrus, Asakawa and Ohbayashi, 1986 (Nematoda: Heligmosomidae) from a Chinese rodent, Apodemus peninsulae (Rodentia: Muridae); with comments on Heligmosomoides polygyrus polygyrus (Dujardin, 1845) and related species
    J Massoni
    Universite Pierre et Marie Curie, Paris, UMR 7207 CNRS MNHN UPMC Centre de recherche sur la paléobiodiversité et les paléoenvironnements CR2P MNHN, Paris Cedex, France
    Parasite 19:367-74. 2012
  3. pmc Development of three PCR assays for the differentiation between Echinococcus shiquicus, E. granulosus (G1 genotype), and E. multilocularis DNA in the co-endemic region of Qinghai-Tibet plateau, China
    Belgees Boufana
    Cestode Zoonoses Research Group, School of Environment and Life Sciences, University of Salford, Salford, Greater Manchester, United Kingdom
    Am J Trop Med Hyg 88:795-802. 2013
  4. pmc Drivers of Echinococcus multilocularis transmission in China: small mammal diversity, landscape or climate?
    Patrick Giraudoux
    Chrono Environment, University of Franche Comte, CRNS, Besancon, France
    PLoS Negl Trop Dis 7:e2045. 2013
  5. doi First report of Echinococcus shiquicus in dogs from eastern Qinghai-Tibet plateau region, China
    Belgees Boufana
    Cestode Zoonoses Research Group, School of Environment and Life Sciences, University of Salford, M5 4WT, Greater Manchester, UK
    Acta Trop 127:21-4. 2013
  6. pmc Transmission ecosystems of Echinococcus multilocularis in China and Central Asia
    Patrick Giraudoux
    Chrono environment lab, UMR6249, University of Franche Comté and CNRS, Besancon, France
    Parasitology 140:1655-66. 2013
  7. ncbi Echinococcosis: serological detection of patients and molecular identification of parasites
    Akira Ito
    Asahikawa Medical College, Department of Parasitology, Midorigaoka, Higashi, Asahikawa, Hokkaido, Japan
    Future Microbiol 2:439-49. 2007
  8. pmc Specific IgG responses to recombinant antigen B and em18 in cystic and alveolar echinococcosis in china
    Tiaoying Li
    Institute of Parasitic Diseases, Sichuan Centers for Disease Control and Prevention, Chengdu 610041, Sichuan Province, China
    Clin Vaccine Immunol 17:470-5. 2010
  9. ncbi Description of a new species of Heligmosomoides (Nematoda: Heligmosomidae) parasitic in Microtus limnophius (Rodentia: Cricetidae) from Rangtang, Sichuan, China
    J Massoni
    Universite Pierre et Marie Curie, Paris, UMR 7207 CNRS MNHN UPMC Centre de recherche sur la paléobiodiversité et les paléoenvironnements CR2P MNHN, 57, rue Cuvier, BP 48, 75231 Paris Cedex 05, France
    Parasite 17:17-22. 2010
  10. pmc Frequency distribution of Echinococcus multilocularis and other helminths of foxes in Kyrgyzstan
    I Ziadinov
    Institute of Parasitology, University of Zurich, Winterthurerstrasse 266a, CH 8057 Zurich, Switzerland
    Vet Parasitol 171:286-92. 2010

Scientific Experts

  • Tiaoying Li
  • Minoru Nakao
  • Anke Dinkel
  • Wulamu Mamuti
  • Jean Mathieu Bart
  • Ning Xiao
  • J Knapp
  • Belgees Boufana
  • Thomas Galewski
  • A Vaniscotte
  • Christine M Budke
  • Akira Ito
  • Patrick Giraudoux
  • J Massoni
  • I Ziadinov
  • Philip S Craig
  • Francis Raoul
  • Hu Wang
  • J P Quere
  • M C Durette-Desset
  • F Audebert
  • Qian Wang
  • A Mathis
  • P Deplazes
  • Hao Wen
  • P R Torgerson
  • Xiu min Han
  • David R J Pleydell
  • Xin Hua Chen
  • Jiamin Qiu
  • David Pleydell
  • Eve Afonso
  • Yan Xie
  • Jean Pierre Quéré
  • Xiuming Han
  • Iskender Ziadinov
  • Xiaohui Feng
  • Li Li
  • Yurong Yang
  • R Nurgaziev
  • K Abdykerimov
  • B Mutunova
  • A Ito
  • Bing hui Wei
  • Hui xia Cai
  • P S Craig
  • Yu Fang Liu
  • Xiao Ma
  • Dominique A Vuitton
  • O T Kuttubaev
  • T A Abdyjaparov
  • Yu Rong Yang
  • Donald P McManus
  • A Rysmukhambetova
  • D Trachsel
  • F Mark Danson
  • Yasuhito Sako
  • Jing Ping Zhang
  • Shu Seng Zheng
  • Xu Dong Ma
  • Zhao Xia Zhang
  • Jin Hui Zhang
  • Xiao hui Feng

Detail Information

Publications34

  1. ncbi Nothing is perfect! Trouble-shooting in immunological and molecular studies of cestode infections
    Akira Ito
    Department of Parasitology, Asahikawa Medical University, Asahikawa 078 8510, Japan
    Parasitology 140:1551-65. 2013
    ..The dilemma of whether developing countries should develop their own diagnostic tests or rely on commercially available kits is also discussed...
  2. pmc Redescription of Heligmosomoides neopolygyrus, Asakawa and Ohbayashi, 1986 (Nematoda: Heligmosomidae) from a Chinese rodent, Apodemus peninsulae (Rodentia: Muridae); with comments on Heligmosomoides polygyrus polygyrus (Dujardin, 1845) and related species
    J Massoni
    Universite Pierre et Marie Curie, Paris, UMR 7207 CNRS MNHN UPMC Centre de recherche sur la paléobiodiversité et les paléoenvironnements CR2P MNHN, Paris Cedex, France
    Parasite 19:367-74. 2012
    ..agrarius and A. peninsulae, previously identified by Asakawa et al. (1993) as H. neopolygyrus, are considered to be Heligmosomoides incertae sedis. This is the first report of H. neopolygyrus in A. peninsulae from China...
  3. pmc Development of three PCR assays for the differentiation between Echinococcus shiquicus, E. granulosus (G1 genotype), and E. multilocularis DNA in the co-endemic region of Qinghai-Tibet plateau, China
    Belgees Boufana
    Cestode Zoonoses Research Group, School of Environment and Life Sciences, University of Salford, Salford, Greater Manchester, United Kingdom
    Am J Trop Med Hyg 88:795-802. 2013
    ..shiquicus coproDNA using necropsy-positive fox samples...
  4. pmc Drivers of Echinococcus multilocularis transmission in China: small mammal diversity, landscape or climate?
    Patrick Giraudoux
    Chrono Environment, University of Franche Comte, CRNS, Besancon, France
    PLoS Negl Trop Dis 7:e2045. 2013
    ..We tested the hypotheses that human disease distribution can be explained by either the biodiversity of small mammal intermediate host species, or by environmental factors such as climate or landscape characteristics...
  5. doi First report of Echinococcus shiquicus in dogs from eastern Qinghai-Tibet plateau region, China
    Belgees Boufana
    Cestode Zoonoses Research Group, School of Environment and Life Sciences, University of Salford, M5 4WT, Greater Manchester, UK
    Acta Trop 127:21-4. 2013
    ..Faecal samples from two Tibetan foxes were shown by PCR to harbour both E. multilocularis and E. shiquicus DNA. One of these dual E. multilocularis and E. shiquicus infections in a Tibetan fox was confirmed by sequencing...
  6. pmc Transmission ecosystems of Echinococcus multilocularis in China and Central Asia
    Patrick Giraudoux
    Chrono environment lab, UMR6249, University of Franche Comté and CNRS, Besancon, France
    Parasitology 140:1655-66. 2013
    ..multilocularis. This approach may be used at fine spatial scales to characterize other poorly known transmission systems of the large Eurasian endemic zone, and help in consideration of surveillance systems and interventions...
  7. ncbi Echinococcosis: serological detection of patients and molecular identification of parasites
    Akira Ito
    Asahikawa Medical College, Department of Parasitology, Midorigaoka, Higashi, Asahikawa, Hokkaido, Japan
    Future Microbiol 2:439-49. 2007
    ..Both the serological detection of the disease and molecular phylogeny will be discussed in perspective...
  8. pmc Specific IgG responses to recombinant antigen B and em18 in cystic and alveolar echinococcosis in china
    Tiaoying Li
    Institute of Parasitic Diseases, Sichuan Centers for Disease Control and Prevention, Chengdu 610041, Sichuan Province, China
    Clin Vaccine Immunol 17:470-5. 2010
    ..4% in cases with alveolar lesions containing central necrosis and 66.7% in patients with early alveolar lesions that measured </=5 cm...
  9. ncbi Description of a new species of Heligmosomoides (Nematoda: Heligmosomidae) parasitic in Microtus limnophius (Rodentia: Cricetidae) from Rangtang, Sichuan, China
    J Massoni
    Universite Pierre et Marie Curie, Paris, UMR 7207 CNRS MNHN UPMC Centre de recherche sur la paléobiodiversité et les paléoenvironnements CR2P MNHN, 57, rue Cuvier, BP 48, 75231 Paris Cedex 05, France
    Parasite 17:17-22. 2010
    ..There are no illustrations of this species and the female has not been described; for that reason, it is not possible to compare it accurately with our specimens...
  10. pmc Frequency distribution of Echinococcus multilocularis and other helminths of foxes in Kyrgyzstan
    I Ziadinov
    Institute of Parasitology, University of Zurich, Winterthurerstrasse 266a, CH 8057 Zurich, Switzerland
    Vet Parasitol 171:286-92. 2010
    ..This is because the prevalences of E. multolocularis and Mesocestoides spp. were the highest (and hence had fewest zero counts) yet the parasite distribution nevertheless gave a better fit to the zero-inflated models...
  11. doi A real-time multiplex-nested PCR system for coprological diagnosis of Echinococcus multilocularis and host species
    Anke Dinkel
    Department of Parasitology, University of Hohenheim, Emil Wolff Str 34, 70599 Stuttgart, Germany
    Parasitol Res 109:493-8. 2011
    ..This newly developed real-time multiplex-nested PCR system is a specific, sensitive and reliable method for the detection of E. multilocularis and host species in faecal samples for epidemiological purposes...
  12. doi Role of dog behaviour and environmental fecal contamination in transmission of Echinococcus multilocularis in Tibetan communities
    A Vaniscotte
    Department of Chrono Environment, UMR UFC CNRS 6249 aff INRA, University of Franche Comte, 25030 Besancon Cedex, France
    Parasitology 138:1316-29. 2011
    ..multilocularis peri-domestic cycles and to identify behavioural parameters required to model E. multilocularis transmission in this region...
  13. pmc Post-treatment follow-up study of abdominal cystic echinococcosis in tibetan communities of northwest Sichuan Province, China
    Tiaoying Li
    Institute of Parasitic Diseases, Sichuan Centers for Disease Control and Prevention, Chengdu, Sichuan Province, People s Republic of China
    PLoS Negl Trop Dis 5:e1364. 2011
    ..The current prospective study aimed to assess the effectiveness of community based use of cyclic albendazole treatment in Tibetan CE cases, and concurrently monitor the changes of serum specific antibody levels during treatment...
  14. pmc Widespread co-endemicity of human cystic and alveolar echinococcosis on the eastern Tibetan Plateau, northwest Sichuan/southeast Qinghai, China
    Tiaoying Li
    Institute of Parasitic Diseases, Sichuan Centers for Disease Control and Prevention, Sichuan Province, People s Republic of China
    Acta Trop 113:248-56. 2010
    ..These chronic cestode zoonoses constitute an unparalleled major public health problem for pastoral Tibetan communities, and pose great difficulties for adequate treatment access and effective transmission control in such remote regions...
  15. pmc Genetic polymorphisms of Echinococcus tapeworms in China as determined by mitochondrial and nuclear DNA sequences
    Minoru Nakao
    Department of Parasitology, Asahikawa Medical College, Asahikawa, Hokkaido 078 8510, Japan
    Int J Parasitol 40:379-85. 2010
    ..shiquicus was segregated into the Tibetan Plateau by colonising alpine mammals and its mitochondrial locus has evolved without bottleneck effects...
  16. ncbi Geographic pattern of genetic variation in the fox tapeworm Echinococcus multilocularis
    Minoru Nakao
    Department of Parasitology, Asahikawa Medical College, Asahikawa, Hokkaido 078 8510, Japan
    Parasitol Int 58:384-9. 2009
    ..The nuclear DNA sequence for the immunodominant B cell epitope region of ezrin/radixin/moesin-like protein (elp) was extremely conservative, indicating that the elp antigen is available for immunodiagnosis in any endemic areas...
  17. ncbi [Field trial on rapid detection of echinococcosis by dot immunogold filtration assay (DIGFA) with whole blood sample]
    Xin Hua Chen
    Surgical Lab, The First Affiliated Hospital Medical School Zhejiang University, Hangzhou 310031, China
    Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi 23:90-2. 2005
    ..To establish a rapid, simple and reliable assay with samples of whole blood for diagnosis and epidemiological study on hydatidosis...
  18. ncbi Recent advances in characterization of Echinococcus antigen B
    Wulamu Mamuti
    Department of Parasitology, Asahikawa Medical College, Japan
    Parasitol Int 55:S57-62. 2006
    ..This review focuses on recent advances in molecular biological and immunological characterization of AgB from both of E. granulosus and E. multilocularis...
  19. ncbi Echinococcus multilocularis: developmental stage-specific expression of Antigen B 8-kDa-subunits
    Wulamu Mamuti
    Department of Parasitology, Asahikawa Medical College, Japan
    Exp Parasitol 113:75-82. 2006
    ..multilocularis vesicles, protoscoleces, and immature adult worms. Possible functions of different expression manners are also discussed...
  20. ncbi EmsB, a tandem repeated multi-loci microsatellite, new tool to investigate the genetic diversity of Echinococcus multilocularis
    J M Bart
    Parasitology Department, SERF Team Equipe Santé Environnement Rural Franche Comté, Faculty of Medicine, University of Franche Comte, France
    Infect Genet Evol 6:390-400. 2006
    ..e. sensitivity, repetitiveness and discriminatory power. It will permit assessing the genetic polymorphism of E. multilocularis and to investigate its spatial distribution in detail...
  21. ncbi Dual infection of animal hosts with different Echinococcus species in the eastern Qinghai-Tibet plateau region of China
    Ning Xiao
    Department of Parasitology, Asahikawa Medical College, Asahikawa, Japan
    Am J Trop Med Hyg 75:292-4. 2006
    ..shiquicus in the liver. The high susceptibility of some mammalian hosts to the parasites and the high prevalence of the three co-endemic species probably increase the chance of mixed infections in the eastern Tibetan plateau...
  22. pmc The evolutionary radiation of Arvicolinae rodents (voles and lemmings): relative contribution of nuclear and mitochondrial DNA phylogenies
    Thomas Galewski
    Laboratoire de Paléontologie, Phylogénie et Paléobiologie CC064, Institut des Sciences de l Evolution UMR 5554 CNRS, Universite Montpellier II, France
    BMC Evol Biol 6:80. 2006
    ....
  23. ncbi A molecular phylogeny of the genus Echinococcus inferred from complete mitochondrial genomes
    M Nakao
    Department of Parasitology, Asahikawa Medical College, Asahikawa, Hokkaido 078 8510, Japan
    Parasitology 134:713-22. 2007
    ..Host-parasite co-evolution comparisons suggest that the ancestral homeland of Echinococcus was North America or Asia, depending on whether the ancestral definitive hosts were canids or felids...
  24. ncbi Molecular characterization of a novel gene encoding an 8-kDa-subunit of antigen B from Echinococcus granulosus genotypes 1 and 6
    Wulamu Mamuti
    Department of Parasitology, Asahikawa Medical College, Midorigaoka Higashi 2 1 1 1, Asahikawa 078 8510, Japan
    Parasitol Int 56:313-6. 2007
    ..granulosus genotypes 1 and 6 by PCR, and named as EgG1AgB8/5 and EgG6AgB8/5, respectively. The phylogenetic relationship of these genes with other genes encoding the antigen B 8-kDa-subunit monomers was also discussed...
  25. pmc Assessment of use of microsatellite polymorphism analysis for improving spatial distribution tracking of echinococcus multilocularis
    J Knapp
    Institute of Parasitology, Vetsuisse Faculty, University of Bern, Bern, Switzerland
    J Clin Microbiol 45:2943-50. 2007
    ..The complex EmsB pattern was able to discriminate isolates on a regional and sectoral level, while avoiding overdistinction. EmsB will be used to assess the putative emergence of E. multilocularis in Europe...
  26. pmc Canine echinococcosis in Kyrgyzstan: using prevalence data adjusted for measurement error to develop transmission dynamics models
    I Ziadinov
    Institute of Parasitology, University of Zurich, Winterthurerstrasse 266a, CH 8057 Zurich, Switzerland
    Int J Parasitol 38:1179-90. 2008
    ..multilocularis of 11% (CIs 6-29%) compared with 26% in free-roaming dogs (CIs 17-44%) and independently within these groups hunting dogs were more likely to be infected than non-hunting dogs...
  27. pmc Species identification of human echinococcosis using histopathology and genotyping in northwestern China
    Tiaoying Li
    Sichuan Centers for Disease Control and Prevention, Chengdu, Sichuan Province, PR China
    Trans R Soc Trop Med Hyg 102:585-90. 2008
    ....
  28. ncbi Evaluation of three PCR assays for the identification of the sheep strain (genotype 1) of Echinococcus granulosus in canid feces and parasite tissues
    Belgees S Boufana
    Biomedical Sciences Research Institute, University of Salford, United Kingdom
    Am J Trop Med Hyg 78:777-83. 2008
    ..Improvement of PCR tests for Echinococcus species and subspecific variants should rely on the use of less-conserved genes and development of protocols that improve the quality and quantity of DNA extracted from feces...
  29. pmc Landscape composition and spatial prediction of alveolar echinococcosis in southern Ningxia, China
    David R J Pleydell
    Department of Chrono Environment, UMR UFC CNRS 6249 aff INRA, Universite de Franche Comte, Besancon, France
    PLoS Negl Trop Dis 2:e287. 2008
    ..Habitat availability is thought to affect arvicoline population dynamic patterns and definitive host-intermediate host interactions. Arvicoline habitat correlates with AE prevalence in Western Europe and southern Gansu Province, China...
  30. ncbi [Epidemiological survey on echinococcosis in Darlag County of Qinghai Province]
    Xiu min Han
    Qinghai Institute for Endemic Disease Prevention and Control, Xining 811602, China
    Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi 27:22-6. 2009
    ..To make an epidemiological investigation on echinococcosis in Darlag County, Guoluo Tibetan Autonomous Prefecture of Qinghai Province...
  31. ncbi A canine purgation study and risk factor analysis for echinococcosis in a high endemic region of the Tibetan plateau
    Christine M Budke
    Institute for Parasitology, University of Zurich, Winterthurerstrasse 266a, CH 8057 Zurich, Switzerland
    Vet Parasitol 127:43-49. 2005
    ..Male dogs were more likely to be infected with Echinococcus spp. than female dogs (P<0.05) and dogs allowed to roam were more likely to be infected with E. multilocularis (P<0.05)...